LOCUS       NZ_CP147753             5596 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 20200226S3 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP147753
VERSION     NZ_CP147753.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40242871
            Assembly: GCF_037251875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   wang,j.
  TITLE     Investigation of Rapid Emergence of Colistin Resistance in a Newborn
            Infected with KPC2-Producing Hypervirulent Carbapenem-Resistant
            Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   wang,j.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2024) Peking university people's hospital, Peking
            university people's hospital, 11 Xizhimen South Street, Xicheng
            District, Beijing, Beijing 100044, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.130
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 12X
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037251875.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:05:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,900
            CDSs (total)                      :: 5,778
            Genes (coding)                    :: 5,589
            CDSs (with protein)               :: 5,589
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 189
            CDSs (without protein)            :: 189
            Pseudo Genes (ambiguous residues) :: 0 of 189
            Pseudo Genes (frameshifted)       :: 83 of 189
            Pseudo Genes (incomplete)         :: 114 of 189
            Pseudo Genes (internal stop)      :: 27 of 189
            Pseudo Genes (multiple problems)  :: 34 of 189
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147753.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="20200226S3"
                     /isolation_source="bone marrow"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="China:Shijiazhuang"
                     /lat_lon="37.27 N 113.33 E"
                     /collection_date="2020-02-26"
                     /collected_by="lm"
     gene            162..488
                     /locus_tag="WDV70_RS29450"
     CDS             162..488
                     /locus_tag="WDV70_RS29450"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            514..1059
                     /locus_tag="WDV70_RS29455"
     CDS             514..1059
                     /locus_tag="WDV70_RS29455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            1125..1475
                     /locus_tag="WDV70_RS29460"
     CDS             1125..1475
                     /locus_tag="WDV70_RS29460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(1545..1811)
                     /locus_tag="WDV70_RS29465"
     CDS             complement(1545..1811)
                     /locus_tag="WDV70_RS29465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1795..2019)
                     /locus_tag="WDV70_RS29470"
     CDS             complement(1795..2019)
                     /locus_tag="WDV70_RS29470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(2686..2898)
                     /locus_tag="WDV70_RS29475"
     CDS             complement(2686..2898)
                     /locus_tag="WDV70_RS29475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2905..3414)
                     /locus_tag="WDV70_RS29480"
     CDS             complement(2905..3414)
                     /locus_tag="WDV70_RS29480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<3449..4153)
                     /locus_tag="WDV70_RS29485"
                     /pseudo=""
     CDS             complement(<3449..4153)
                     /locus_tag="WDV70_RS29485"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4092..4436)
                     /locus_tag="WDV70_RS29490"
     CDS             complement(4092..4436)
                     /locus_tag="WDV70_RS29490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(4426..4602)
                     /locus_tag="WDV70_RS29495"
     CDS             complement(4426..4602)
                     /locus_tag="WDV70_RS29495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            5242..5400
                     /locus_tag="WDV70_RS29500"
     CDS             5242..5400
                     /locus_tag="WDV70_RS29500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     Typing          complement(4689..4765)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          4954..5351
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(2571..4102)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccccggagct ccatccatcc cacggaaaaa cacccgccac tgatgccctc agcgcactgc
       61 acatcatgta caaaccactc cctccctcct tttttattgc gtttcttaat gcgcaatggt
      121 agctttgtgc gcaacaaaaa acgcaatagc aagggggggt aatgagagaa agaacaaata
      181 tcatgctaga tataggatta aagcaatcct tacaacggct ttcgaataaa accaaaatga
      241 gtctaagtga tcttattaac cagagattat caggttcact ttcagttgaa gaacgcattg
      301 gcgaaaagcc tgaatggatg agaaaccttg atccctatct cgccaatcac ttttcaaata
      361 ttgagttacc gctcccttca ccattaaaag aataccaaga aaaaatggat gagatagatt
      421 caattattaa tgacatgatg gttctaaagg aatatttcag agctaagttt tacgacgaaa
      481 taaaataggc acgctatacg gaggcggaga attatgtttt ttaaacggta tttaacggag
      541 aaggaaatat acaaggcagc actcccaatt gttaggcttc aagggagagt taacacttca
      601 ttacgttttt tatccaaata ctcagatgta aacaaaaatg gattaattgc attgcatctt
      661 aatttattac attttatctc atacaacata cacggtgtag agtctctgaa aatcactcaa
      721 gcaggcataa acaaagctgt acaacaaagc cctcacgcag atgaaataaa aaatattgcg
      781 caggcaatag gacgcaaagg acctgagtac atcgaaggat taattagaaa cccatggggg
      841 aagaaaaatg ataatccact aatatttata atgagcattg aagaaagtat aaaagaaacc
      901 cttaactcag gttatatgtt atatagagta gctcccataa ctgagggtga agcagctgct
      961 ttcatggatg ttcttaaaca attattatta tttgaggata attttcgcct ttttggtaga
     1021 gacttggttt ataaaataga aaaattaaaa atcagataac aaagtatggt gaacgaaaaa
     1081 tgaaagagag gatagtgaaa tgtgtaatac caagattctc aactatgtat tggttaattg
     1141 cgttcctagc atttatattc cccggtcgat cttttgctga cggttacacc gcttactacc
     1201 gatgtaatgg tactcaggaa gcatctctta ctgttaaaga taacaaacta agtatggttg
     1261 taggtctttc taagtttaat aggcaactgg atacttatag attcaaagaa aagggaatcg
     1321 aagtagtaat gcagagagct gcttctgatg ataactctga ggccataatg tcttttatta
     1381 tgcctgacgt taacaatatg accttattag ttaaaaataa cggctacatt atcgttaaga
     1441 caacctgctg gttaactaaa accgacaaac agtagaacac gtagttagtc gagtatttac
     1501 cataatacct gttgtcggca tcaaaaaagc ccgcatagcg ggcttcagtc gtaaacttcg
     1561 cggcgatgtc caatcgtggc ggcaaggata acaagctcgt catctcggat ctcacacaga
     1621 acgcgataat ctcccacccg atagcgccag aactcgccca gctcaccttt gagcggcttc
     1681 cctgactggc gaggatcggc agcaactgca atgcgtaaac tcataaaatc cacaatccgt
     1741 cgtgcgttct gtttgtccat cttgcgtaac gatttgagcg cccgatcgga atagttaatc
     1801 gtccagacca agtgacttcc tcacgtcttc agacgaatgc actgcttcat caccacggcg
     1861 aacgcgtgca gcagtttctg ctgaaagata ataatcctcc aggtcttcca gatgcgccag
     1921 tattgcttcc cgcgcataaa acgtctttgt tctgccggtt tgcttcgcca gcgagtccag
     1981 acgggactca atctcatcag ataaccggat agcaagcata taaacctccc gaacaccatt
     2041 tgctatacat gtatatcaac agtaccacaa tcaacctaaa agaacaaaaa ataatcttgt
     2101 tagcccctct tgataatccg taacgcggtc aatatatcta gcttgagggc tttcctctta
     2161 actattgaaa ggataacgat tatgggtctt aagccgggtc cgaaacgaat tgccgattca
     2221 acaggggaac ctgataaacg ccagcgtgac aataaaaaaa cgccgggaaa tactgacaaa
     2281 ttgaaaccca gcaaatcttc aaaaaaataa gtcagtagtt aaaagccctc cggggctttt
     2341 ttgtatctgc cgttcacgct gattaaacat aacacccgtt gcccgcaccg ccccggacgg
     2401 gttcgccgcg aacggggtgg cagtcaggta aaaccggcaa caaaccaccc gataaaccgc
     2461 cccgttcgcg acagataaat tttacctggc tgcatagcca tgcagccagg tgaatgacat
     2521 ccggtttcag cgtaaatgcc cgatgagaac cctgttttcc tgcatttcag cccagcgacg
     2581 gaccgtcata gcttcgctgc cgggaacgcg cttccgtcag ttccttttcg tgctgccgtt
     2641 gctcgtgcgc ccgttctgcg gccacggcca tctcatgacg ctgcactacc ggctcaaacg
     2701 ctgcagttgc ccgctcaagc gttccacctg ctgactcaag cgcgtcacgc gctcgctcag
     2761 cgccgcgttc tcccgttgca taagccctga cattttccgc cattccccga aggcgctctc
     2821 ccactcgtcc agccttttcg agtagtcctg ctgtagctgc tctaatgcgc tcagcaactg
     2881 tttttccagc tcggtcatcg gcacttactc cttcaccagt ctcagcgcgt tctgcttcga
     2941 ccgcccgttc ccgaccgtcc agctctgcgt cctgtctacg gccgtcccct tcggcagcac
     3001 cagaaaacgt ccgttgctgt cttccaggta cgtcacgccc caggtcttcg cgttcagctt
     3061 cgacagcgct gcgttctgcc ggtcgatttc ggccagattt gacgctatca agctcccctg
     3121 ataccacaac accccgctca ggctggcgaa cagcagtcct gacaccatcg tgatggtcag
     3181 ccatgtccgc ccgaccatac gcatcacgct caggcggttc gactgcatgg ccgcgctcat
     3241 accctgctcg tgggcgcgga tggcggcgct gattctcttc ccgctttcgc tcagttccgc
     3301 tctgacagac ttctcgtgct cgctgaatgc ggctttcagc atttcgccgg tactctgctg
     3361 ctgcgctttc gatttctgct ctaagtcctt cgccagcgtt aaaagactgt tcatagatgg
     3421 ctcccttaag tcggatgttt cgccctccgt ccgggtcggc aatgctgatg ctgctctttg
     3481 tggtgcgcac gacctcaaaa cctgcgcttt ccagcgcctc agtgacgtcc tgacgcgttt
     3541 taagctcccc tgacgaggcg agggccagta agccccgcgt aatagcctct gcggcttcct
     3601 gcttcgtttt gggtaacgaa gatggcgtca ccagcgcgcg ccggttctcc ggtgcgttcg
     3661 ggtcgtgcag ccccagtcgc ccgttcacca cggtctgcca cgcatcgatg cgcggacggt
     3721 ctgcccggtc gtagtacggc tggagacgcc tgccggtcag cagttccgtg ttcggtacca
     3781 gaaaattcag ttccagccgc cccttgtccc ggtgttcaac ccacagcacg ctgtactggt
     3841 ctttatcgag tccgggcatc agaacccgct cgaagctcgc catcagcttt tcacgctggc
     3901 cgggcggtaa atcctgttca gcaaaagaca ggacgccgga ggtgtacttt ttggcgtagg
     3961 gcgaggcgtc gataagctcc cggacctcct ccggctttcc ctgcaggacg atggccccgt
     4021 cgcgctggcg gtctttcccc agcagataat ccaccggccc ggcaccgccg cctcgccctc
     4081 gcggatgaaa cttaacgatc atcgtctgcc cccttttcca gcacggcatg ccgcagccgc
     4141 tcgagttcgg catcaatggc catcagcgcg gccaccacct gaacccggtc agcgcctgac
     4201 cactggccgc cgttaatttt ccgggcgatc tggttgaggt tgttccccat cccggcaagc
     4261 tggcgcagca aaaccggatc gatggacggc agccgcaacg accgcgccgg gcgcgtatcg
     4321 agacaggttt cccgcatcca caccgccagt tgccttccat tgcagcgctc cagcagctgc
     4381 tggtactccc catcggtcac ccggatacct aaaaaacggg tacgcttaac cgccatcctc
     4441 tcctccgata tccgcaaaat gtgaccgggc tgaacgctca agctctgcca tctgctcccg
     4501 gcggtaatct tcctcctgtg cctgacgcat cagatttgcc atcactgccg gatgccagga
     4561 tctttttttt acgacgagcg gcttcttctt cggcttctgc attatccctc cttcgcaaac
     4621 gccggacggc acggaaaagt tttccgggtc gctccggcgt tggacaagcg ccagcgcgtc
     4681 agtggtgtta gcgggtttcg gggcgcagcc ctgaaccagt cacagcgttt acgctgtgta
     4741 tactggctta ctatgttggc atgagagcac gattcatgaa agtgcctcat gtggcaggtg
     4801 tgatatgtgg ctcaggagcg tccgcaatac catgcacgga cagatgaaat gacgcctcct
     4861 cgctcactcg gtagctgcgc tcctgccgtt cggctgcggc gggcgtgacc gactcacgga
     4921 taacgggata aagcaaaggc gctcaaatca gaaagggccg gagaaaagga tgcggcgttg
     4981 ccgtttttcc ataggctccg cctcccctga cgagcatcac agaaatggac gctcaagtca
     5041 gaggaggacg aaacccgaca ggacttaaag atgccggtga tttctctccg gcggctccct
     5101 cgtgcgctct cctgttccga ccctgccgtt taccggatac ctgttccgcc tttttccctt
     5161 tcgggaagcg tggcgctttc tcatagctca cgctgctggt atctcggctc ggtttaggtc
     5221 gttcgctcca agctgggctg tatgcacgaa ccccccgttc agcccgactg ctgcgccctt
     5281 tccggtaact atcatcttga gtccaacccg gacggacacg caaaaacgcc actggcagca
     5341 gccattggta actggatata tacgagagag atattgagag gtcttgaagt gggtgcctaa
     5401 ctgcggctac actgaaagaa cagttttggt atctgcggtc tcgtataccc agttacccgg
     5461 ttaagcagtt ccccaactga cttaaccttc gatcaaaccg cctccccagg cggttttttc
     5521 gtttacagtg caagagatta cgcgcagaaa aaaaggatct caagaagatc ctttaaatga
     5581 tcgtcagttc tgttat
//
