LOCUS       NZ_CP147745            11970 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 20200219S1 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP147745
VERSION     NZ_CP147745.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40242869
            Assembly: GCF_037249905.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   wang,j.
  TITLE     Investigation of Rapid Emergence of Colistin Resistance in a Newborn
            Infected with KPC2-Producing Hypervirulent Carbapenem-Resistant
            Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   wang,j.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2024) Peking university people's hospital, Peking
            university people's hospital, 11 Xizhimen South Street, Xicheng
            District, Beijing, Beijing 100044, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.130
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 12X
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037249905.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:11:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,846
            CDSs (total)                      :: 5,725
            Genes (coding)                    :: 5,554
            CDSs (with protein)               :: 5,554
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 171
            CDSs (without protein)            :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 68 of 171
            Pseudo Genes (incomplete)         :: 111 of 171
            Pseudo Genes (internal stop)      :: 24 of 171
            Pseudo Genes (multiple problems)  :: 32 of 171
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147745.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="20200219S1"
                     /isolation_source="bone marrow"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="China:Shijiazhuang"
                     /lat_lon="37.27 N 113.31 E"
                     /collection_date="2020-02-19"
                     /collected_by="lm"
     gene            324..569
                     /locus_tag="WDV71_RS29165"
     CDS             324..569
                     /locus_tag="WDV71_RS29165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            843..1214
                     /gene="tolA"
                     /locus_tag="WDV71_RS29170"
     CDS             843..1214
                     /gene="tolA"
                     /locus_tag="WDV71_RS29170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            1211..1576
                     /locus_tag="WDV71_RS29175"
     CDS             1211..1576
                     /locus_tag="WDV71_RS29175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            1751..2029
                     /locus_tag="WDV71_RS29180"
     CDS             1751..2029
                     /locus_tag="WDV71_RS29180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            2017..2331
                     /locus_tag="WDV71_RS29185"
     CDS             2017..2331
                     /locus_tag="WDV71_RS29185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2495..2923
                     /locus_tag="WDV71_RS29190"
     CDS             2495..2923
                     /locus_tag="WDV71_RS29190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            2949..3128
                     /locus_tag="WDV71_RS29195"
     CDS             2949..3128
                     /locus_tag="WDV71_RS29195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3155..3685)
                     /locus_tag="WDV71_RS29200"
     CDS             complement(3155..3685)
                     /locus_tag="WDV71_RS29200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3692..4423)
                     /gene="mobC"
                     /locus_tag="WDV71_RS29205"
     CDS             complement(3692..4423)
                     /gene="mobC"
                     /locus_tag="WDV71_RS29205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(4423..6387)
                     /locus_tag="WDV71_RS29210"
     CDS             complement(4423..6387)
                     /locus_tag="WDV71_RS29210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(7871..8020)
                     /locus_tag="WDV71_RS29215"
     CDS             complement(7871..8020)
                     /locus_tag="WDV71_RS29215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(8105..8362)
                     /locus_tag="WDV71_RS29220"
     CDS             complement(8105..8362)
                     /locus_tag="WDV71_RS29220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(8372..10057)
                     /locus_tag="WDV71_RS29225"
     CDS             complement(8372..10057)
                     /locus_tag="WDV71_RS29225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(10162..11649)
                     /gene="ltrA"
                     /locus_tag="WDV71_RS29230"
     CDS             complement(10162..11649)
                     /gene="ltrA"
                     /locus_tag="WDV71_RS29230"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     BGC             3372..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          7190..7768
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3696..4423)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 ggggactttc accccttgat ctctaaccgg tctcccggcg cacactgtac atatacacag
       61 tataattatt accttactcc tcccgttttc aggtgtcaat atatgagatt agttctcatc
      121 ttcaggcaca aaaaagcgcc cggagccacc tgtaagcaga tcgcgtcttt tcccgaaaat
      181 ttctcatggc aacaaatttt attatttaag acattcatca cacttacatt tcagatggta
      241 tagacacatc tgaaagctaa ctggcatgag gagaggacct gcggttaaac tgttcgggaa
      301 acctgagacg cccggaggcc actatgacgc acctgaaact gactcccgct gaagaaaagc
      361 gcctggcgga cgcctttgtc gctcaggcac tggccatggg cctgaaaggg aacaaaacgc
      421 tgccaaaggc ggtaaaacaa caggtgcggg aaagggtgct tcgtgccatt caggacgaac
      481 gtgaagccag gcgggcgcag gaaatgcgtc gggagaaata cgaggctacg gaaaccacct
      541 tcacctggca gccacagcgc cgccggtaag gggtatttac cataacgtcg cattgcccgc
      601 accgctcaga cggtctgagg gcgtttccgg cggctacggg ataaaacagg caacaaacca
      661 cccggaaaac cgccggaaac gcgcccggta aattgtaacc cgctgcatgc ctatgcacgc
      721 aactgcatgg cgggccgttt ggtgtaaaaa cgatacgata agccgtgttt atgtaaatga
      781 acgtggccgt aaagagtgac aaatatacat gtaacatttt ttactgacag gtcatcaacc
      841 cgatgcgttt aatacccttt atcctgctgg cattgtgcag ccccttactc ctgacggcct
      901 gtaagcattc caccacccag ccgccgaaaa tttatactct ggataagtca tctaaaatca
      961 gtagctacgc tgcggccatc aagaccgctg ttgaaatgca gatgtttgaa ccagaggcct
     1021 atgcgggaaa aatgtgcacc gtgcggatat ccatacaacg tgatggcact ctgaacagcg
     1081 ccattgctga aggaggagat ccgaaactct gtaaagcagc aatatcagcc gttacccacg
     1141 ccaaaattcc gccggcaccg gatgaaaata cctggcaaat attcaaaaac gcccctctgg
     1201 atttcagacc atgaaagctt ttactctgac agtgctggcg gccatgttcc tcactccggt
     1261 acatgcgata ccactaaaac ccgtaaccac actgcagcca acctacccgg tacaggccgc
     1321 agcaaaacgc atcgaaggtc aggtgaccgc cacgtttgat attctggacg atggacgagt
     1381 ggacaacgtg tcgcttaccg gctgtcacct tttttttcgg gaaacgctga cggcaatgag
     1441 gcactggcta tatcccgcag ggcacccggc aatgcgacag gttataaatt ttgaattcag
     1501 tctgaaaaat gacggcttac aggatccgcg accagtcacc acccaaaagc tcaatgatga
     1561 tggttgccgc cagtaacagg tcctttaacc cgcaggctgc aatagtcctg gatgatgagt
     1621 gagcctgtag cagccctgat tagtggaagc gaagctgaaa aatgggcagc aaaagccgta
     1681 tttctggcaa atagcgccgc cacggattaa agtagtcatg tgtaatacat atgactacaa
     1741 aggggctttt atgtcatcag caacatccat caggctcgat gaagaactta aggaccggct
     1801 gaaaacgctg gccgatgacc ggcaccgctc agcgcacgcg ctaatgctgg aggcaatcac
     1861 cgaatacatc gaccgggaag agaagcgcag ccagtacctg cgggacggac aggccgcatg
     1921 gcagcactat caggaaacgg gtctgcacct tactgcggaa gaagcggaag cctggataag
     1981 tacctgggga actgagaacg agcaggatgc tccgccatgc cacaggtaac gatttccgcg
     2041 ctggcacagc gcgatttaca acgccttcag gactttctca aaaccaaaaa tcggctggca
     2101 gccagaaaag gaggtgaggt gatcgtccgg gctatccagc aactgaaaac attgccagac
     2161 attggccgcc cggtgccgtt tctgccgctg gaatataagg aactggtgat cgggtttggc
     2221 gacagtggct atgtgatgct ctaccgccac gaccgggaaa tggaccagat tgtgatcgtc
     2281 accgtcaggc atcagaaaga atccgggtat ccgggggcag acagcctcta ataaccgatg
     2341 acgggggcgc aattcattgc gtggccgtcg ccggtttgac aagcgcagcg cgtcagaaaa
     2401 ccacatcctg aacggaaaaa cgggtaaaat tcagcgatac atttaccgtg agaaattaca
     2461 cacctttcac acccgataaa atcaggggta tacagtggcg aaaacattgc tcaacctttc
     2521 acaggcagca caagccgcgg gcattaccag aagaacgcta tacaatcatg taaaacaggg
     2581 aaaggttacc gtgtcacgtg acgggaaaaa caaccctgtc gtggatgtgt cagaactgat
     2641 ccgtgtatac ggaaatgtga atatccctga gaaacaaatt cccggcattt cacaccgtga
     2701 aaatacacaa aaaaatttcc cacaggaaca gcttctggca atgcaaaagg aactggccga
     2761 tctccggcag gctgtcaccc tgatgctgga agataaaaca tcccgggaag aagaacgccg
     2821 ccagcacgat gatgagcgcc ggaaactcca ggccgaagtg gacagattga caacagaact
     2881 gacacagaag aaaaaacgct tctggtccgg ctggtttagc tgagaactta ccgaagggaa
     2941 tggagaacat gacgaagcag gaaaaaactg ccctcaatat ggctaaattc attaaggatc
     3001 agtcgctgct gttgctggaa aagctgaacg agctggacat ggataccccg gccgacatgt
     3061 gcgaacggct tcacgaggat gcggaaacac tctggcaggt gatgcgggat aagctcgggg
     3121 aagaataaca gcaccggcca tgccggtgct ggcgttattt tttgccgaac ggccaccacc
     3181 ggcgggcggg tttgtcctcc ggcagttcag gcaccggcac gttaaaatag cggctgttat
     3241 attcccgcat cagctccgct ttcagcttct cgttcaccgc gctttccccg tgccgctcgc
     3301 tgaccacctc aatcatcgcg ttaatctgct ccggtgagca cttcccggca tcaaccacct
     3361 gcacaccgtt ctcgatggcg aaccgctcca cctttcgata gaggtaatca cacagcttca
     3421 ttcctgtggg tttgtcccgt tcccattgcg gattttcacc ggtgaaaaag tacgtggctt
     3481 tttcgtcgct gttgagccgc tcgtcataca tcccgatgga tttcatcatc gccagatgct
     3541 gggcccggtc gacaaaacag aaaacatacg ccccggtctg ccaggcgagc accaccatgt
     3601 cttccagatg ctcgctggtt ggcataatgc agcgctcgcc gtctttgtct tccacgtcct
     3661 caataaatac catgtacagc gccataatcc ctcactccgt tgtcataaat ttaaagcacg
     3721 cgaacagcct ctcccggtcc agctccaccg ttttgcgggc ctcgccggtc tgtggattca
     3781 cgatttcatc caccgccaga atcgcccgct gtacccggac aagcgtcggc cggtcagggc
     3841 agatgtacca gatttcatcg atgccgttcg cccggcggtt gaacagatac ccggacacca
     3901 cttcctgata ccgcctgcgg ctcttgacgg tgcgctctac ctcaaatgcc accgttctgc
     3961 cgtccagctc cgccagcgcg tccggcactt tcatcccttt cagggccata cggtgcaggt
     4021 accgccagcc ggtggccccc cggccctcca tggagaggcg cagggactgc acggccagcc
     4081 ggtgcggcag ctgcgccgcc gacacccttg acggctgaaa cggaataacg tccgtcagcg
     4141 gctcgtcctc gtcgaacgag aacgccaccc cgtgcggcgt cagcccccac acgctgacct
     4201 gacggccgtc aaccgggtat ttcgcccgga ccagcagccc tttcgccacc agactgtcca
     4261 ggaagcggta aaccggctgt accgccgcaa tccccagccg tcgcgcaacc agctccgccg
     4321 tggaacacag ctcttcccgc agccagcgca gggtcagccg tgttttctcc gcgttccgcg
     4381 cctgccgctt cgcgtggctg acgttgtact gctccagtgt catcagtaaa gccccgcaaa
     4441 atcatcatcc gcttttggtc cgccgccgtc ggccggaggc aggtactccg ccagcgggtc
     4501 cgttttcgcc agcaccggga aggttttcag cgaaatttcc cggcgctcaa cccgcagcgg
     4561 gctgctgaac gccagccgcg ccacgcccag cccggtcatc accgcgaacc cgtcaggcag
     4621 gtgttgcagg gtgttcacgt caaacagcgg ccgcgcctcc ttctggatga agctgtcgcc
     4681 gctgatgtgc tccacgttcc ccgcctcccg gctggcccgg cggcgctcca cgtccacccg
     4741 gatctccccg gtctggcccg ccgcccactg cgcggactcc tggctggccg cgcgatagaa
     4801 ccagcggatc ggcgtgttat ccagtaccgt ggttttcaca aaatcagcgg gcagatcctg
     4861 cccgcactga ccgaagtcgc ccagcgactg gtgcgcgagc agcaggttgc agtcccggct
     4921 gcgcagcgtc cccagcgcgt tcagcacgta cttcgacagc agatatttaa tctcatcgag
     4981 catgatgctc gcgtgcggcc acctccggaa ctcatcccgc gcaatgatga tttgcgcgca
     5041 gcgggcaaac agcatcttct gcacccggat caccgcctca tcgtccatcg agccgatgac
     5101 gtacaggcag ccgccgccgt tgattatccc cgccacatcg ataccgctgt ccgtctgcag
     5161 gacgctcagg tcggccacgt tctccagctt ggtgatcagc cccttcacgt cctttttcag
     5221 ggcctccggc aacgcgtacg ccgccgccag cacctgctga atgtttgccc cctgcgggaa
     5281 ctgctccgca atcagcttcg ccgctttctg ctcgctgatg cggtagtggt ccgccacgtc
     5341 gccggtctca ctcaggttaa acccggcaac cagcagattc ttcagggcgt actggtcgag
     5401 atcgcggaac agattcagct gcgccggttt ccccggccgc agatccagca gggtgaagtc
     5461 cgggcagtgc gcccggaaga cgtgcggcgc ccacgcgtcc cctttggggt cgaagtacac
     5521 cacagcgtcc ccgaaatcgc gtacgcagcg aatgagcgcg ttggtggcca tcgcgctctt
     5581 gccgctcccg ggcaggccga ggatctgaat attggtcttg cgccaggtat cacgagggac
     5641 cagcaccggc tggccctgct catccttacc cagaaacacc gcctgctgtt tccgggcctg
     5701 ctccgtggaa aaatcggcca gcgcgttaac cggtaccggc tccccgaaat gcaggtggcg
     5761 gacgtctgtc atttctccgg tccgctggct gctgcgccgg ctctcctgat tcagtttcgc
     5821 gttcaccgcc tcgtcagcgg ccgcaaaatg accatcaagc agcaggaacg ccagcagccg
     5881 cgccagaagg acgccggcga caagccctcc ggcacaccag gctatcccga acaggttagc
     5941 cggctgcagc aggtaataat ctgcgttcgc atagaggtcc gggcgcagga cggtttctcc
     6001 ccagctctcg ataaacacgg cgacaaccag cccccccaga agggccagac cggcagcgcc
     6061 ggtcagccag tacgcgtagt tccagacatg tcgccgtgcc ctgaccatcc gcccgggtga
     6121 atccagcgag gccagcaccg aaaatggcac agtccggtta ttatcgcgct tatagcgatc
     6181 ggcatactcc cgccgcgcct tatggctcag ccccacaaaa cgcattcggc cggcccagag
     6241 ttcagccaga ccgaaaaaca ggccgataac cagaaaaaaa ccggccagac tgtaatactc
     6301 ccggtacagc cagctgaccc acgggtaaat ctgccgggcc agctccggga tatgccactg
     6361 ccaggcaagc gaatccgtat taaacatgca atctctctcc ttcagacgac gggtttaccg
     6421 gcgtgatgcg cagcccccgc tgcacaagcc gcggacatgc cccaaggcat ggcggcggcg
     6481 gtcacgtccg tggtgattgc gttcagcttt caccgcggag gccagcctct ttccgcccca
     6541 tgcggaaaag gcgcagcccc ggaggaacaa cgagcacgcc ccagcgtgtg agaggaaaag
     6601 gcggcacccc agtgcggtct tttccggtgt tccggccccc gtcagagttc gccatagcga
     6661 gcggctgacc acccgcaaaa tcaggagcag ctttgctgct tcgtcaggtg cgtccagcac
     6721 cgtggatttt tgtgggtggt caggcgtgag cctgacgggg gccgggtgaa tttctggcgt
     6781 ggccggcgtc cagccggaca gagccagaaa gaggggaaaa gaccgtgcgt cggtggcgtc
     6841 cagccagcgt aagcaaggtc ttttcccctg aagtcagcca cgccccagcg gggatgactt
     6901 gcatttctcc gcaaggagaa aaaagcaaca gagataggtt aacgtaaaat gttcagcgaa
     6961 aaaccgacat ggttcaccta tcctgataat tgatcgtcag ggaatagaaa gacttaatca
     7021 ggggacaata tcccacatca gcgcgtccgg tgaccgtctt gcaggtgccg gggaaattgc
     7081 gcctcctcgc tcactcggtc gctacgctcc gggcgtgaga ctgcggcggg cgctgcggac
     7141 acatacaaag ttacccacag attccgtgga taagcagggg actaacatgt gaggcaaaac
     7201 ggcagggccg cgccggtggc gtttttccat aggctccgcc ctcctgccag agttcacata
     7261 aacagacgct ttttcggtgc atctgtggga gccgtgaggc tcaaccatga atctgacagt
     7321 acgggcgaaa cccgacagga cttaaagatc cccaccgttt ccggtaggtc gctccctctt
     7381 gcgctctcct gttccgaccc tgccgtttac cggatacctg tccgccgttc tcccttgcgg
     7441 gaagtgtggc gctttctcat agctcacaca ctggtatctc ggctcggtgt aggtcgttcg
     7501 ctccaagctg ggctgtaagc aagaactccc cgttcagccc gactgctgcg ccttatccgg
     7561 taactgttca cttgagtcca acccgggaaa gcacgcaaaa cgccactggc agcagccatt
     7621 ggtaactggg agttcgcaga ggatttgttt agctaaacac gcggttgctc ttgaagtgtg
     7681 cgccaaagtc cggctacact ggaaggacag atttggttgc tgtgctctgc gaaagccagt
     7741 taccacggtt aagcagttcc ccaactgact taaccttcga tcaaaccacc tccccaggtg
     7801 gttttttcgt ttacagggca aaagattacg cgcagaaaaa aaggatctca agaagatcct
     7861 ttgatctttt ctactgaacc gcgatccccg tcagttcaga ggaggacgat ggtgccaccg
     7921 ttccgccctg aacatcccgg atataatttg cctgacatgc ggccagaaga aagccagcaa
     7981 caatcaatat aaaaagaaaa atagtcttta cctttttcat atactttccc tgtatttaaa
     8041 gactccggca ttcatccctg aacaccggaa gtcagtattt caaatcatcc ttgataattt
     8101 tttgttacca gttaccgtcc cgataatcaa aagctatata acaattatcc acatcaggtt
     8161 ttatttgatt tttaaaaaat ggctgtagta aaggaaccca ttctttcttc aaatcaaact
     8221 caccattatt aatattatcc tttaaaggca ttcccaagct ttcaatgaca gaaccatcgt
     8281 caccaaagtc tttagaatac tcttcccctt taaactcttc agttttctta tcaaaccatg
     8341 tcaaacgtaa tttaagcccc atatttacct ctcaaagata ttttttaatg ttccgttttg
     8401 gatctggccc tttaacctgc ttacccgttt tgggatcaaa tgcgcctagg tgttcgccat
     8461 cactggcacg atacccttca agctcaccgt gctgggaatc ccattcataa attttacgtt
     8521 ttttatcgcc ataccatcgg gcccgcttac caccaccacc ttgtttaggt gttttaggat
     8581 cacctcgctt taactctcca agtcccttta tgtcttctgt tttaggtact ggatgataat
     8641 catggccgta gtctttagtc cccttgcgag gcttgttctt ttcctcattt aacttatttt
     8701 ctgcatcctt cgcttttttc tccttttgtt tccggctctc catcgccccg gtaagtgcag
     8761 ccttatctct ggataaggat tcattcattg cgtttaaaga tgcctgagcc gcttttaatt
     8821 tctcattctg attctgaata tccttatcaa gatagcgaac ctgatcctgc cacccggagc
     8881 catattcatg aggcgggtta taagtcacaa aatcatcttt atttgcctct gcctctttta
     8941 cctttttatc ggcttcctga acagcagccc ttgcaccggg gagcgccttc tctgtcgatg
     9001 caatgcggct gtttaaggtc gcaatatttt tatcttcggc atccagctca gctttcgctt
     9061 tatcatagtc tcttttcagc ccctcttccg gatgagcggc gtcccatgcc tgctggcgac
     9121 gcttttcttc ctcctgacgc tgttttacct gtgccggggt aagaacatct gtcaccgaca
     9181 cataaaccgg cttctgtccg gtctctttcg ggaaacgaat aacggcctca cgggagttac
     9241 caccggctgt aaaaccagcc ggacgtgaat cacctttttc agcaacaatg ccttttggcg
     9301 gggctttcgc tgtcggaaca cctttcggta cgctcacctg cagagacggg agacccggaa
     9361 tcgacacact gaataccccc ggacgttttg tcggtttcgc atccaccaca gggacactca
     9421 ttggccgacc tgcgacaacc gcaatatgct gccgctcatc cttcaccaca tccacaaccc
     9481 gttgtctgac acgaaccgtc gcctggtcca gtggtaaagt acttgccgga gtctccgtca
     9541 ccgcatcggc cggtaatgac gtcataattt ttgacatcat tttcggatca tcttttgcaa
     9601 tctcagaagg cagcacaccg tagatcgcaa tcccccacag accaaactta aacggcccct
     9661 tcagggcagc cagcacatca gcaacggcgg ccgacaacgc atcgccggaa actgataaag
     9721 ccagtccttc agcccccgga gtagccaggg caggcagacc gaaggccatc gcagtggccg
     9781 aagactgtcc gccaccagag ctaccgctat gattgccgct gttaccacca ttaccaccgg
     9841 gtttactccc gccgttacca aactcccccg gattatagtt atgaatatct ccattcggcg
     9901 tatgcgtcgt accccagccc ggcccgctgt ttggatccgt accaccacct gatgaagggc
     9961 cacctttacc agacgtaccg ttcacattcc cactggcctg accaccatta tgtcccagac
    10021 ctgaattacc cggacctcga ccgtctccac cactcatacc gttttcctct ttgacattta
    10081 aaacaataaa ttaaagccaa catgtcgcgg tagggatacc cgttaccgga taccccccgc
    10141 acagatcccg gcgtgcgcga tttacgcacc gggctcctgc ctcgggtgtc tggcggtgaa
    10201 ccgctccaca ggccatggat gaagaacccg aacccttggt agccatgcgg ctgccagttt
    10261 gtttgctttc gtccaggtcg tatcatcctt ctggctcctg cgcctgagcg cccggcgcca
    10321 gaggtttgtt acgtgtgtcc tgaacttctg catggtgggg aagttgcccg gtaccgagtg
    10381 atagttcagg tatccctgaa ccactctcct gagccatttt ccctgttcgg ggattgagta
    10441 atgccagcgc cttcgcagac cgtctttgat ggctttcaga gttgccgtca tccgatcccg
    10501 gcgggtcttt cgtatcagca tgaacctgcc gttgcgatct ttcccgctga tgtgcgtgaa
    10561 cccgaggaag ttgaacgttt ctggtttgcc ttttcccctg atggcacggt tttcggcagc
    10621 gaagcggccg aactccatca gacgggtttt ctccgggtga accgtgagtc cgaactccct
    10681 cagtctgcgc tgcatggcta tacggaagcg ccgggcatcg tatcgtttgt cgaacccgat
    10741 gacgatgtca tcggcgtatc tgaccattac cacattgcct gtggcatagc gacgtcgcca
    10801 ctgatgcgcc cacagatcga agacgtagtg gaggtatatg tttgccagca gcggtgagat
    10861 gaccgcaccc tgtggggtgc cttcctccgt tgctcgccat tgaccctcct ccgacgtccc
    10921 ggctgtgagc cacttacgta tgagcctgat taccctccgg tcgccgatcc gatgctctgt
    10981 gaacctgatc agccattcgt ggctcaccct gtcgaagaac tgactgatgt cggcatccag
    11041 tacccagttt acgttagtgc gtaccagccc tgtggccagt gcgtccagtg catcgtgctg
    11101 gcttcgcccg ggtctgaacc cgtatgagaa ccccataaag tcgttttcat agactgcgtt
    11161 caggattttc accagcgcat actggacgat cttgtcctcc agcgaggcga tgccgagcgg
    11221 gcgttgtttt ccatccgctt ttgggatgta gtgacgcctg ccgggctgcg ccctgtagct
    11281 gccctgatgt agcctccggt gcagatctgt tatgttgttc ttcatgtttc cggcgtagtc
    11341 catccacctg atgccatcca ctccggcggc cgctttcctg ctcagggaga ggaatgcggc
    11401 ttccagtgct tcgactgtca gcaggtggaa caatgctgta aaccgttctt tcttccgctg
    11461 cttcgcagct tcccgcacgc gtgacagcct ctgtgacatg ctttcccggc tctgtgtccg
    11521 gcgcatgtgt ggctgttccg cgttcccctt ggccccgctc cttcgctcca ctgactccgc
    11581 tcctttcggg ttgttcgcct gcttcgccgc tactatgagc gagtccgact tctcctctcc
    11641 gtacatcacc ggctatgact cctcgtcttc ccggtgcggg ccatctccga cactggcaga
    11701 tggtcagagg ggagatctcc cggttcccgc gtagagatcg tattgacatg ccagggtctc
    11761 agaccccgcc gggtccatgt ggcactcgca gtgtcgcacc ctatgatgtt gccttccgtt
    11821 aacagtacaa cgtcggcacc cggtaattta atatacattt cgtggctcaa tggctggcct
    11881 gtcaacaccc ctgtcaacgc ttcgccccat acctcgcggt atgcaacgca tgactcgggg
    11941 accttgtgga ttgctggtcc ttcaatggtc
//
