LOCUS       NZ_CP147693             3923 bp    DNA     circular CON 05-APR-2024
DEFINITION  Staphylococcus aureus strain BSN102 plasmid pUSA01, complete
            sequence.
ACCESSION   NZ_CP147693
VERSION     NZ_CP147693.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38637690
            Assembly: GCF_037217515.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Pre-publication release of Staphylococcus aureus str BSN102 whole
            genome sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2024) Pharmacy Practice, Wayne State University,
            259 Mack Ave, Detroit, MI 48201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2024
            Assembly Method                   :: SPAdes v. FEB-2024
            Assembly Name                     :: BSN102 contigs
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: CP043386, CP053637, CP014442
            Genome Coverage                   :: 170.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037217515.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 18:30:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,896
            CDSs (total)                      :: 2,814
            Genes (coding)                    :: 2,734
            CDSs (with protein)               :: 2,734
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 40 of 80
            Pseudo Genes (incomplete)         :: 42 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 17 of 80
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147693.1.
            Bacteria and source DNA available from Andrew D. Berti, Wayne State
            University, Detroit MI  USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BSN102"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pUSA01"
                     /geo_loc_name="USA: Detroit, MI"
                     /lat_lon="42.33 N 83.05 W"
                     /collection_date="2021-08-16"
                     /collected_by="Andrew D. Berti"
     gene            1..147
                     /locus_tag="UXQ38_RS14460"
                     /old_locus_tag="UXQ38_14460"
     CDS             1..147
                     /locus_tag="UXQ38_RS14460"
                     /old_locus_tag="UXQ38_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_053861352.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVRFCCKVK
                     KYS"
                     /name="replication protein"
     gene            complement(175..849)
                     /locus_tag="UXQ38_RS14465"
                     /old_locus_tag="UXQ38_14465"
     CDS             complement(175..849)
                     /locus_tag="UXQ38_RS14465"
                     /old_locus_tag="UXQ38_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="WP_001105991.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDIWL
                     RKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMVKVIKAFKLKPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIML
                     AS"
                     /name="IS6-like element IS257 family transposase"
     gene            865..1794
                     /locus_tag="UXQ38_RS14470"
                     /old_locus_tag="UXQ38_14470"
     CDS             865..1794
                     /locus_tag="UXQ38_RS14470"
                     /old_locus_tag="UXQ38_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_148562100.1"
                     /translation="MLKLYYKFNFATEPNVSYADILEILKIKKAFNVKQCGNILEFKPT
                     DEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVIEKVIKEKPKARWLFLTLSTKNA
                     IDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRSTEVTVNKNDGSYNQHMHVLLCVE
                     NAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAIKPNRKGDKDIESAIKETSKYSV
                     KSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLKQKHKILNLDDVEDGNLINASDE
                     DKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            2377..2565
                     /locus_tag="UXQ38_RS14475"
                     /old_locus_tag="UXQ38_14475"
     CDS             2377..2565
                     /locus_tag="UXQ38_RS14475"
                     /old_locus_tag="UXQ38_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     gene            complement(2891..3472)
                     /locus_tag="UXQ38_RS14480"
                     /old_locus_tag="UXQ38_14480"
     CDS             complement(2891..3472)
                     /locus_tag="UXQ38_RS14480"
                     /old_locus_tag="UXQ38_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     Typing          1865..2029
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          907..1794
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.888"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcaatata atactactag aagtataacc gaaaatcaag ataataaaac gttaaaagat
       61 atgacgaaaa gtgggaaaca acgcccatgg agagaaaaga aaatagataa tgtaaggttc
      121 tgttgcaaag taaaaaaata tagctaacca ctaatttatc atgtcagtgt tcgcttaact
      181 tgctagcatg atgctaattt cgtggcatgg cgaaaatccg tagatctgaa gagacctgcg
      241 gttcttttta tatagagcgt aaatacactc aatacctttt aaagtattct ttgctgtatt
      301 gatactttga taccttgtct ttcttacttt aatatgacgg tgatcttgct caatgaggtt
      361 attcagatat ttcgatgtac aatggcagtc aggtttaagt ttaaaagctt taattacttt
      421 aaccattgct accttcgttg aaggtgcctg atctgtaatt accttttgag gtttaccaaa
      481 ttgtttaatg agacgtttga taaacgcata tgctgaatga ttatctcgtt gcttacgcaa
      541 ccaaatatct aatgtatgtc cctctgcatc aatggcacga tataaatagc tccattttcc
      601 ttttattttg atgtacgtct catcaatacg ccatttgtaa taagcttttt tatgcttttt
      661 cttccaaatt tgatataaaa ttggggcata ttcttgaacc caacggtaga ccgttgaatg
      721 atgaacgttt acaccacgtt cccttaatat ttcagatata tcacgataac tcaatgcata
      781 tcttagatag tagccaacag ctacagtgat aacatccttg ttaaattgtt tatatctgaa
      841 atagttcata cagaagactc ctttttgtta aaattatact ataaattcaa ctttgcaaca
      901 gaacctaatg taagctatgc agatatacta gaaattttaa aaatcaaaaa ggcttttaat
      961 gtaaaacaat gtggtaatat tttagaattt aagccaactg atgaaggcta tttgaagtta
     1021 cataagacat ggttttgtaa atcaaaatta tgtccggttt gtaattggag acgtgctatg
     1081 aaaaatagtt atcaagctca aaaagtgatt gaaaaagtaa ttaaggaaaa gccaaaagca
     1141 cgttggttgt ttttaacact ttcaacaaaa aatgcgatag atggagatac tttagaacaa
     1201 agtttgaagc atctaactaa agcatttgat aggttgagta gatataaaaa ggttaaacaa
     1261 aatcttgttg gatttatgcg ttcaacagaa gttaccgtta ataaaaatga cggtagttat
     1321 aatcagcaca tgcatgtttt gttatgtgtt gaaaatgcat attttagaaa aaaagagaat
     1381 tatataactc aagaagaatg ggttaattta tggcaaagag cattacaagt tgactatcga
     1441 cctgttgcta atgttaaagc gatcaaaccg aatagaaaag gcgataaaga cattgaatcg
     1501 gcaatcaaag agacctcaaa atattcggtt aaatcatctg attttttaac tgatgatgat
     1561 gaaaaaaatc aagaaattgt aagtgattta gaaaaaggtt tgtatcgaaa acgtatgtta
     1621 agttatggtg gattgcttaa acaaaaacat aaaattttaa acttagacga tgtcgaagat
     1681 ggtaatttga ttaatgcaag tgatgaagat aaaacaacag acgaagaaga aaaagcacat
     1741 tcaattaccg caatttggaa tttcgaaaag caaaattatt atttaagaca ttagtgttga
     1801 ctaatgtctt ttttgttgat tttttataaa aaagtactgt cttatttttg tgacaaatgc
     1861 tgtatgtagt gtcacaaaaa taagacaaac gcaatatatt gtgtcacaaa aataagacag
     1921 tacagctttg tatgatatca ctttaaaata ccttaaaacc cctggaattt ctggctttgc
     1981 cagacctatc atttttgaat gatagcaaat tctccttatg ctcttacgga gtttttagag
     2041 aaaaattaaa aattctcgat ttttgataaa aaacgccctg caggaattta gaaaaacatg
     2101 tggaagtttt aaaggatttt atgctaattt ttaatttgca tgtaaatggg cagtgttaaa
     2161 aaattagtca ctggttttgc tgaaattttt gtttgtgaaa ttaccatata tttatttggc
     2221 tcatatttgc gttttaagag cttgtatata tttttaagtt aaaaacctat atgatacccc
     2281 ctaaaccttt aaaatacccc cttaaaactc aaaataaagg tatttataat ttgagtaagt
     2341 cttgtgataa agttttttaa aaaaggagtg tttattttga ataaaaacac agtgttagat
     2401 gaaggttacg ttatagcaac tattttaaat gttttctttt tacttggatt aatttttata
     2461 tctcatttgg aaaatttata tatattaatt ccgtatgtta ttttgatggg aataaatgct
     2521 atttatttgg ttgttaaggt tatgaatttc aaaaagaata attagaatat ttgaggggat
     2581 tttgaaacga gtttcttctt gttttcacac tgttttttta ttcctattgg tgttgttgct
     2641 tactttttgt ttttctataa agatgatgct tttgacgaga tggaagaaaa atatgattat
     2701 catgaagata ataaaaaata gacgacgcat ttatgccgag aaaatttatt gatgttgaga
     2761 agaaccctta actaaacttg cagacgaatg tcggcatagc gtgagctatt aagccgacca
     2821 ttcgacaagt tttgggattg ttaagggttc cgaggctcaa cgtcaataaa gcaattggaa
     2881 taaatagtat ctaaagtaac ccgtatttaa gattacattg tacatccttc gaaaccatag
     2941 tagctgcgat tccagctagg tttcctttta tcccaatttt agctaatttt tttgctacgc
     3001 cctttaagtt tttggcttta acatcttcaa aaactgctgt gattatgttt ccagttaaaa
     3061 gttctccata tgagtttaaa acttcagaat agaaacagtc accgctattt ttgtagttcc
     3121 caccaactgc tctaggtgat attgaactag ttttcttttt attctctatg tcgtttttta
     3181 gtttttggaa tccttcagga acatatccat atcgagattt ggcattttct agattaacat
     3241 ctttagctgt tccgtcttta tctaaaataa taattttgcc atatatgtat tttagttctt
     3301 tttcaatttc tttttcgttt aaagtttcaa cgtttttatc tgcaatgtta tttttgtttt
     3361 gggcttgagc ttctttgaaa ttaaaatcca agaccattga aagtatggtt aaaatactta
     3421 aagatactac taaaatcttt cttagtgata tactgttttg aattgcattc attgtaaatg
     3481 cccccttgtt ttgttattta aatgaattca atataatatt aacatataaa aacaaaggag
     3541 tttaatatga ataagttttt ttactttttg attattttat tatctgtatt tttaattttt
     3601 aattttgtta attcgatttt atttgataat ccttttgact ttttagaaag ttttatttat
     3661 tcattggttt tcacattaat ttatttaggt ttagagggtc ataaaaagaa gaaggaatag
     3721 gttgtttttt gaaacgagtg tgaacgagtt tcttcttgtc ttgatactat atagaaataa
     3781 ctcgatttta tatatatagc tgtaactgtt gatattacag tgtttaaacg tgtttttgtg
     3841 cgtgaaagga aaatttgaca ataaaaaacc ccagttatat tattaaggtg tcgaatctta
     3901 aataatactg ggggtctttt tat
//
