LOCUS       NZ_CP147689             2469 bp    DNA     circular CON 05-APR-2024
DEFINITION  Staphylococcus aureus strain BSN97 plasmid pCN1, complete sequence.
ACCESSION   NZ_CP147689
VERSION     NZ_CP147689.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38637685
            Assembly: GCF_037217645.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Pre-publication release of Staphylococcus aureus str BSN97 whole
            genome sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2024) Pharmacy Practice, Wayne State University,
            259 Mack Ave, Detroit, MI 48201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2024
            Assembly Method                   :: SPAdes v. FEB-2024
            Assembly Name                     :: BSN97
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: CP029678, CP003981, CP014442
            Genome Coverage                   :: 141.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037217645.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 18:28:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,973
            CDSs (total)                      :: 2,891
            Genes (coding)                    :: 2,808
            CDSs (with protein)               :: 2,808
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 46 of 83
            Pseudo Genes (incomplete)         :: 41 of 83
            Pseudo Genes (internal stop)      :: 13 of 83
            Pseudo Genes (multiple problems)  :: 16 of 83
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147689.1.
            Bacteria and source DNA available from Andrew D. Berti, Wayne State
            University, Detroit MI  USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BSN97"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pCN1"
                     /geo_loc_name="USA: Detroit, MI"
                     /lat_lon="42.33 N 83.05 W"
                     /collection_date="2021-08-10"
                     /collected_by="Andrew D. Berti"
     gene            84..818
                     /gene="erm(C)"
                     /locus_tag="UXQ65_RS14845"
                     /old_locus_tag="UXQ65_14845"
     ARG             85..815
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1262..1738
                     /locus_tag="UXQ65_RS14850"
                     /old_locus_tag="UXQ65_14850"
     CDS             1262..1738
                     /locus_tag="UXQ65_RS14850"
                     /old_locus_tag="UXQ65_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000664727.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     ARG             85..818
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.18"
                     /coverage="100.0"
     Typing          1263..1748
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.591"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attatcaacc aaacaaaaaa taagtggtta taatgaatcg ttaataagca aaattcatta
       61 taaccaaatt aaagagggtt ataatgaacg agaaaaatat aaaacacagt caaaacttta
      121 ttacttcaaa acataatata gataaaataa tgacaaatat aagattaaat gaacatgata
      181 atatctttga aatcggctca ggaaaaggcc attttaccct tgaattagta cagaggtgta
      241 atttcgtaac tgccattgaa atagaccata aattatgcaa aactacagaa aataaacttg
      301 ttgatcacga taatttccaa gttttaaaca aggatatatt gcagtttaaa tttcctaaaa
      361 accaatccta taaaatattt ggtaatatac cttataacat aagtacagat ataatacgca
      421 aaattgtttt tgatagtata gctgatgaga tttatttaat cgtggaatac gggtttgcta
      481 aaagattatt aaatacaaaa cgctcattgg cattactttt aatggcagaa gttgatattt
      541 ctatattaag tatggttcca agagaatatt ttcatcctaa acctaaagtg aatagctcac
      601 ttatcagatt aaatagaaaa aaatcaagaa tatcacacaa agataaacag aagtataatt
      661 atttcgttat gaaatgggtt aacaaagaat acaagaaaat atttacaaaa aatcaattta
      721 acaattcctt aaaacatgca ggaattgacg atttaaacaa tattagcttt gaacaattct
      781 tatctctttt caatagctat aaattattta ataagtaagt taagggatgc ataaactgca
      841 tcccttaact tgtttttcgt gtacctattt tttgtgaatc gattatgtct tttgcgcatt
      901 cgcttctttt ctatataaat atgagcgaag cgaataagcg tcggaaaagc agcaaaaagt
      961 ttcctttttg ctgttggagc atgggggttc agggggttat ctaacgtaaa tgccgagcga
     1021 aagcgagccg aagggtagca tttacgttag ataaccccct gatatgctcc gacgctttat
     1081 atagaaaaga agattcaact aggtaaaatc ttaatatagg ttgagatgat aaggtttata
     1141 aggaatttgt ttgttctaat ttttcactca ttttgttcta atttctttta acaaatgttc
     1201 tttttttttt agaacagtta tgatatagtt agaatagttt aaaataagga gtgagaaaaa
     1261 gatgaaagaa agatatggaa cagtctataa aggctctcag aggctcatag acgaagaaag
     1321 tggagaagtc atagaggtag acaagttata ccgtaaacaa acgtctggta acttcgtaaa
     1381 ggcatatata gtgcaattaa taagtatgtt agatatgatt ggcggaaaaa aacttaaaat
     1441 cgttaactat atcctagata atgtccactt aagtaacaat acaatgatag ctacaacaag
     1501 agaaatagca aaagctacag gaacaagtct acaaacagta ataacaacac ttaaaatctt
     1561 agaagaagga aatattataa aaagaaaaac tggagtatta atgttaaacc ctgaactact
     1621 aatgagaggc gacgaccaaa aacaaaaata cctcttactc gaatttggga actttgagca
     1681 agaggcaaat gaaaaacaag aaaatgcatt atctgattat tattctttca aggactagta
     1741 taacataaaa tcgtctacaa atagacaaaa aacctgcacg cttaatgtag atcaaaagct
     1801 taacgcaaat gaaatagatt gacctcccaa taacaccacg tagttattgg gaggtcaatc
     1861 tatgaaatgc gattaagctt tttctaattc gcataagcgt gcaggtttaa agtacataaa
     1921 aaatataatg aaaaaaagca tcattatact aacgttatac caacattata ctctcattat
     1981 actaattgct tattccaatt tcctattggt tggaaccaac aggcgttagt gtgttgttga
     2041 gttggtactt tcatgggatt aatcccatga aacccccaac caactcgcca aagctttggc
     2101 taacacacac gccattccaa ccaatagttt tctcggcata aagccatgct ctgacgctta
     2161 agtgcactaa tgcctttaaa aaacattaaa gtctaacaca ctagacttat ttacttcgta
     2221 attaagtcgt taaaccgtgt gctctacgac caaaactata aaacctttaa gaactttctt
     2281 ttttcttgta aaaaaagaaa ctagataaat ctctcatatc ttttattcaa taatcgcatc
     2341 agattgcagt ataaatttaa cgatcactca tcatgttcat atttatcaga gctcgtgcta
     2401 taattatact aattttataa ggaggaaaaa atatggacat ttttagtatt tttgtaatca
     2461 gcacagtta
//
