LOCUS       NZ_CP147671             4096 bp    DNA     circular CON 05-APR-2024
DEFINITION  Enterobacter hormaechei strain RH3 plasmid p-unnamed-three, complete
            sequence.
ACCESSION   NZ_CP147671
VERSION     NZ_CP147671.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40316311
            Assembly: GCF_037217235.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Pei,H., He,W., Wang,Y., Zhang,Y., Yang,L., Li,J., Ma,Y., Li,R.,
            Li,S., Li,Q., Li,J., Hu,K., Teng,H., Hu,X., Zou,L., Liu,S. and
            Yang,Y.
  TITLE     Insight into a natural novel histidine decarboxylase gene deletion
            in Enterobacter hormaechei RH3 from traditional Sichuan-style
            sausage
  JOURNAL   J Food Sci 89 (1), 566-580 (2024)
   PUBMED   38126118
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Pei,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2024) College of Food Science, Sichuan
            Agricultural University, No. 46, Xinkang Road, Yucheng, Sichuan.,
            Chengdu 625014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. V2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 432.0x
            Sequencing Technology             :: Illumina NovaSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037217235.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 18:49:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,751
            CDSs (total)                      :: 4,636
            Genes (coding)                    :: 4,564
            CDSs (with protein)               :: 4,564
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 8 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            Pseudo Genes (short protein)      :: 1 of 72
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147671.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RH3"
                     /isolation_source="Sichuan-style sausage"
                     /db_xref="taxon:158836"
                     /plasmid="p-unnamed-three"
                     /geo_loc_name="China:Sichuan"
                     /collection_date="2021-05-28"
     gene            331..489
                     /locus_tag="WCO32_RS23720"
                     /old_locus_tag="WCO32_23720"
     CDS             331..489
                     /locus_tag="WCO32_RS23720"
                     /old_locus_tag="WCO32_23720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032670878.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHEKSLLAAAISNWRYTRDKL
                     RGLEVWA"
                     /name="hypothetical protein"
     gene            complement(726..995)
                     /locus_tag="WCO32_RS23725"
                     /old_locus_tag="WCO32_23725"
     CDS             complement(726..995)
                     /locus_tag="WCO32_RS23725"
                     /old_locus_tag="WCO32_23725"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_079839079.1"
                     /translation="MNGQEFRLLLTEHGEKEAVLLFGKKSFVAVPDTQNLYGGCYYLYL
                     EDAHKNELLIELNESDLYPGGAIELQHNGGRKIHCEILKVRWSR"
                     /name="hypothetical protein"
     gene            1077..1310
                     /locus_tag="WCO32_RS23730"
                     /old_locus_tag="WCO32_23730"
     CDS             1077..1310
                     /locus_tag="WCO32_RS23730"
                     /old_locus_tag="WCO32_23730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461706.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_001036746.1"
                     /translation="MNKKTLLAAMMVALVTFAAAGCSSNQEIKTTDDRTIVTDGKPKVD
                     SDTGLVSYKDAHTGRTEQINRDKITNMSELDN"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1332..1559
                     /locus_tag="WCO32_RS23735"
                     /old_locus_tag="WCO32_23735"
     CDS             1332..1559
                     /locus_tag="WCO32_RS23735"
                     /old_locus_tag="WCO32_23735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_000719360.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPTES
                     DSGLLGYKDANGVKQQINKADVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            complement(2089..2298)
                     /locus_tag="WCO32_RS23740"
                     /old_locus_tag="WCO32_23740"
     CDS             complement(2089..2298)
                     /locus_tag="WCO32_RS23740"
                     /old_locus_tag="WCO32_23740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014538403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001632067.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMCGLMQRENA
                     ALSERVTDLSTQVLSLSEQLRRLS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2302..2787)
                     /locus_tag="WCO32_RS23745"
                     /old_locus_tag="WCO32_23745"
     CDS             complement(2302..2787)
                     /locus_tag="WCO32_RS23745"
                     /old_locus_tag="WCO32_23745"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012782630.1"
                     /translation="MNSLLTLAKDLEQKSKVQQQTTGEMLKAAFSEHEKSVRAELNESE
                     KRISAAIHDHDRMLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLTTCGEERRRCVRVNPDAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<2822..3526)
                     /locus_tag="WCO32_RS23750"
                     /old_locus_tag="WCO32_23750"
                     /pseudo=""
     CDS             complement(<2822..3526)
                     /locus_tag="WCO32_RS23750"
                     /old_locus_tag="WCO32_23750"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3465..3788)
                     /locus_tag="WCO32_RS23755"
                     /old_locus_tag="WCO32_23755"
     CDS             complement(3465..3788)
                     /locus_tag="WCO32_RS23755"
                     /old_locus_tag="WCO32_23755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001555769.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGSSGLDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(3897..3975)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.8899999999999995e-34"
     Typing          2..443
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.751"
                     /coverage="85.0"
                     /evalue="1.6999999999999998e-142"
     Typing          complement(1980..3475)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.998"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgttcggctg cggcgagcgg tacggactca cggataaaga cgtggcgggg aaatacgtga
       61 atgcaggagg ggaagaaagg atgcgccgct gccggttttc cataggctcc gcccccctga
      121 caagcatcac agaatctgac gctcaaatca gtggtggcga aacccgatgc ggcttaaaga
      181 taccaggcgt ttccccctga tggctccctc gtgctccccc gcattcctgc ctttcggttt
      241 accggtgtca ttccgctgtt atggccgcgg tttctcattc cacgcctgac actcagttcc
      301 gcgaaggcag ttcgctccag gattgactgt atgcacgaac cccccgttca gtccgactgc
      361 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg aaagacacga aaaatcgcta
      421 ctggcagcag ccattagtaa ctggaggtat acgagagaca aattaagagg tcttgaagta
      481 tgggcctgag agaggctaca ctgaaagaac agttttggtt tctgcggtct cgtataccca
      541 gttacctggt taagcagttc cccaactgac ttaaccttcg atcaaaccac ctccccaggt
      601 ggttttttcg ttttcagagc aagagattac gactgtaacg acaggatcac aagaagatca
      661 tttactcaga gagactaact accactatgt ttgcctatca acatacttag gaaacattac
      721 ttgagttaac gactccacct tacttttagt atctcgcaat gaatcttccg accgccgttg
      781 tgttgaagtt ctattgcgcc accggggtat aaatcacttt cgtttaactc aattaaaagc
      841 tcgttcttgt gagcatcctc taaataaagg taataacacc caccataaag gttctgcgta
      901 tctggcacgg caacaaaaga tttctttccg aacagtaaaa cagcctcttt ttctccatgc
      961 tctgtcagca ataatcgaaa ttcttgtcca ttcaatcctc ttttcgcatg aaaaaacgac
     1021 ctatacttag atggttagtg accatatcca ctttaccttt aagcagggaa cctattatga
     1081 ataaaaaaac gctgttggcg gccatgatgg tcgcgttggt gacctttgcc gctgctggat
     1141 gttcttcaaa ccaagaaata aaaaccacag atgatcgcac tatcgttaca gacggcaaac
     1201 ccaaagttga cagtgatacg ggattagtat cctacaaaga tgcccataca ggccgaaccg
     1261 aacagatcaa ccgtgacaaa attaccaaca tgagtgaact agacaactga tacggataaa
     1321 aggagctggc tttgaaaaag atttttgttt cagtgttcgc agcggcagtt gcgctttcag
     1381 ccctaaccgg ctgcacgcgc acaagctatg ccatccacac caacgacggg cgtacaattg
     1441 tcagcgacgg caagcctaca gaatcagact caggcctgct gggctataaa gacgcaaatg
     1501 gtgtcaaaca gcagatcaat aaagccgacg ttaaagaagt ctctgaaatt ccgcattgag
     1561 gcatcgctct ctccgaaaag ggccgctaat gcggcccttt tctttatctg ccccaacaac
     1621 aggcaaaaaa agcccccgct gggggccaaa tacctgcagt ggctggatag tactgcattc
     1681 ctgatgaagg agacgaataa gtatgggccg gatttttgcg tctgcaggct cggtcgccac
     1741 aatttttccg tacccgccag gcatcatgct tttaccataa catctcttgc ccgcacctct
     1801 ccggacgggt tcagcgcgaa tgcggcgtca gtcctgcaaa accgacagta aaccaccccg
     1861 caaaccgtgc attctgcgcc tgataaatct cacccgactg catagccatg cagccgggtg
     1921 aatggcaccg ggtttcggcg cagatcctca atgaaagccc cgttttatcc ggttatcaca
     1981 gcgacggacc gtcatactcc tgctgacgct gcgatcgttc cagctccagc gctttctggt
     2041 gctgcgctgc gcgctcctgc tcccggtgtg cctcgatgtc gttcagcctt acgacaaccg
     2101 gcgcagctgc tcgcttaaac tcagcacctg cgtgctcaag tccgtgacgc gctcgctcag
     2161 cgctgcgttc tcccgttgca taagaccaca catcgtccgc cattccgcga aggcgctctc
     2221 ccactcgtcc agccttttcg agtagtcctg ctgtagctgc tctaatgcgc tcagcaactg
     2281 tttttccagt tccgtcatgt gctatttccc cgccagtatc atccagctcg aatcctctcc
     2341 gaaccgtccc gcgtccgggt tcaccctcac gcagcggcgt cgttcctctc cgcaggtcgt
     2401 gagctgcacg ccgctgttcc tctcggacag catggcctgc gtgctcttct gctcccggat
     2461 ggtcgtgtaa ttgtcgagta tctgctgccc ctgccaccat agaatgcccg cacttgaggc
     2521 tatcagcagc acggagacca ggacgatggt cagccacgtc tggctgacca tgcgcagcat
     2581 ccctttcgta cgctggctca tggctgagga cagcatccgg tcgtggtcgt ggatggcggc
     2641 gctgattctc ttctcgcttt cgttcagttc cgctctgaca gacttctcgt gctcgctgaa
     2701 tgcggctttc agcatctcgc cggtagtctg ctgctgcact ttcgatttct gctctaagtc
     2761 cttcgccagc gttaaaagac tgttcataga tggctccctt cagtcggatg tttcgccccc
     2821 cgtccgggtc ggcaatgctg atgctgcttt ttgtggtgcg aaccacctca aaacctgcgc
     2881 tttccagcgc ctcagtgacg tcctgacgcg ttttcagctc cccggacgag gctaaggcca
     2941 gtaaaccccg cgtaatcgcc tgagcggctt cctgcttcgt ttccggcagc gcggacggcg
     3001 tgaccagtgc ccgccggttt tccggcgcgt tcgggtcgtg cagccccaga ctgccgttca
     3061 cgatggtctg ccaggcatca atgcgaggac ggtcggcgcg gtcatagtat ggctggagcc
     3121 gtttgccggt cagcagctcc gtgttcggga tcaggaagtt cagctccagc cgccccttgt
     3181 cccggtgctc aacccacagc acgctgtact ggtctttatc gagtccgggc atcagaaccc
     3241 gctcgaagct cgccatcagc gtttcacgct gcccgggcgg taagtcctgt tcggcaaagg
     3301 acagcacccc ggaggtgtac tttttggcgt agggcgaggc gtcgataagc tcccggactt
     3361 cctccggctt cccctgaaga acgctggcac cgtcgcgctg gcggtctttc cccagcagat
     3421 aatcaaccgg gccggcaccg cctccgcgtc cccggggatg aaacttaacg atcatcgtca
     3481 gccccctttt ccagtacggc atgccgcagc cgctcgagtc cggcatcgat ggccatcagc
     3541 gcggcgacga cctgcacgcg gtcgagaccg ctactgccac cggcgttaac ctggcgggca
     3601 atctggttga ggttgttccc catgccggca agctgacgaa gcagcgccgg cgagatcgag
     3661 ggaagtttgc cggcccgggc gggcttctcg tccaggcacg tctgccgcat ccaggccgcg
     3721 agctgtttac cgtcgcagcg ttccagcaga cggcggtgct catcttctgt tacccacatc
     3781 gtgagcattt tgctgcgttt gtctgccaat ccggcctcct gataacgccg ggacgggcgg
     3841 gaaatctttt cccggtcggc gcggtgttgg gcaagccgca ggcgcgtcag tggcatttag
     3901 cgggtttcgg ggcgcagccc tgaaccagtc acgtagcgct agcggagtgt atactggctt
     3961 actatggtgg cacgacggga atcgggatga aatagtcagt catgaaaact gtgagatgag
     4021 gctcaggtgc gtaagaggct gatgcctgtc ccggtggata ttccgcttcc tcgctcactg
     4081 actcgctacg ctcggt
//
