LOCUS       NZ_CP147670             4760 bp    DNA     circular CON 05-APR-2024
DEFINITION  Enterobacter hormaechei strain RH3 plasmid p-unnamed-two, complete
            sequence.
ACCESSION   NZ_CP147670
VERSION     NZ_CP147670.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40316311
            Assembly: GCF_037217235.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Pei,H., He,W., Wang,Y., Zhang,Y., Yang,L., Li,J., Ma,Y., Li,R.,
            Li,S., Li,Q., Li,J., Hu,K., Teng,H., Hu,X., Zou,L., Liu,S. and
            Yang,Y.
  TITLE     Insight into a natural novel histidine decarboxylase gene deletion
            in Enterobacter hormaechei RH3 from traditional Sichuan-style
            sausage
  JOURNAL   J Food Sci 89 (1), 566-580 (2024)
   PUBMED   38126118
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Pei,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2024) College of Food Science, Sichuan
            Agricultural University, No. 46, Xinkang Road, Yucheng, Sichuan.,
            Chengdu 625014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. V2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 432.0x
            Sequencing Technology             :: Illumina NovaSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037217235.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 18:49:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,751
            CDSs (total)                      :: 4,636
            Genes (coding)                    :: 4,564
            CDSs (with protein)               :: 4,564
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 40 of 72
            Pseudo Genes (internal stop)      :: 8 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            Pseudo Genes (short protein)      :: 1 of 72
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147670.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RH3"
                     /isolation_source="Sichuan-style sausage"
                     /db_xref="taxon:158836"
                     /plasmid="p-unnamed-two"
                     /geo_loc_name="China:Sichuan"
                     /collection_date="2021-05-28"
     gene            complement(join(4008..4760,1..162))
                     /gene="lpxO"
                     /locus_tag="WCO32_RS23685"
                     /old_locus_tag="WCO32_23685"
     CDS             complement(join(4008..4760,1..162))
                     /gene="lpxO"
                     /locus_tag="WCO32_RS23685"
                     /old_locus_tag="WCO32_23685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            complement(433..648)
                     /locus_tag="WCO32_RS23690"
                     /old_locus_tag="WCO32_23690"
     CDS             complement(433..648)
                     /locus_tag="WCO32_RS23690"
                     /old_locus_tag="WCO32_23690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(652..1137)
                     /locus_tag="WCO32_RS23695"
                     /old_locus_tag="WCO32_23695"
     CDS             complement(652..1137)
                     /locus_tag="WCO32_RS23695"
                     /old_locus_tag="WCO32_23695"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_141086899.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<1172..1876)
                     /locus_tag="WCO32_RS23700"
                     /old_locus_tag="WCO32_23700"
                     /pseudo=""
     CDS             complement(<1172..1876)
                     /locus_tag="WCO32_RS23700"
                     /old_locus_tag="WCO32_23700"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1815..2138)
                     /locus_tag="WCO32_RS23705"
                     /old_locus_tag="WCO32_23705"
     CDS             complement(1815..2138)
                     /locus_tag="WCO32_RS23705"
                     /old_locus_tag="WCO32_23705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2782..2892
                     /locus_tag="WCO32_RS23710"
                     /old_locus_tag="WCO32_23710"
     CDS             2782..2892
                     /locus_tag="WCO32_RS23710"
                     /old_locus_tag="WCO32_23710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            complement(3147..3608)
                     /locus_tag="WCO32_RS23715"
                     /old_locus_tag="WCO32_23715"
     CDS             complement(3147..3608)
                     /locus_tag="WCO32_RS23715"
                     /old_locus_tag="WCO32_23715"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     Typing          complement(2240..2426)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.316"
                     /coverage="87.0"
                     /evalue="1.25e-50"
     Typing          2406..2892
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.959"
                     /coverage="93.0"
                     /evalue="3.59e-159"
     Typing          complement(336..1825)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.383"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctttgacggc agcttcgaaa agcctgtcat aaacatattt atcggagcca taaatgttga
       61 atgatcaaat aactgcctgg aaaacttttg ttttatttta ccgcgagaat gcgcatacaa
      121 tacactgata agaaaaacaa aaataataat aagtgacagc atgtgtatat tccttgttca
      181 cgccaataat aaaaatctct taaagataac ataatgaatg cacttaaatt ataatctaaa
      241 tgctatttat caatagtata atcgaagctt caccagaaca tatctactac acaggcagag
      301 aggatttcag caggaattat gctgaaatga atcaaaggga cggaccgtga tatacccgct
      361 gtcgctgtga tcgttcgagc tccagcgctt tctgatgccg cgcctcccgc acctgctgca
      421 gctcgacggc attcagccgc tcgacaaccg gcgcagctga tcgctcaaac tctgcacctg
      481 cctgctcaag cccgtgacgc gctcgctcag cgccgcgttc tcccgttgca taagaccaga
      541 catcgtccgc cattccgcga aggcgctctc ccactcgtcc agccttttcg agtagtcctg
      601 ctgtagctgc tctaatgcgc tcagcaactg tttttccagc tctgtcatgt gctatttccc
      661 cgccagtatc atccagctcg aatcctctcc gaaccgtccc gcttccgggt tcaccctcac
      721 gcagcggcgt ctctgctcgc cgcaggtcga gagctgcacg ccgctgttcc gctcggacag
      781 catcgcctgc gtgctcttct gctcccggat gctcacgtaa ttgtcgagta tctgctgact
      841 ctgccaccac agaatggccg cgttcgaggc gatcagcagc acggagacca ggacgatggt
      901 cagccacgtc tggctgacca tgcgcagcat ccctttcgta cgctggctca tggctgagga
      961 cagcttccgg tcgtggtcga ggatggcggc gctgattctc ttctcgcttt cgctcagttc
     1021 cgctctgaca gacttctcgt gctcgctgaa tgcggctttc agcatctcgc cggtagtctg
     1081 ctgctgcgct ttcgatttct gctctaagtc cttcgccagc gttaaaagac tgttcataga
     1141 tggctccctt cagtcggatg tttcgccccc cgtccgggtc ggcaatgctg atgctgcttt
     1201 ttgtggtgcg taccacctca aaacctgcgc tttccagcgc ctcagtgacg tcctggcggg
     1261 ttttcagctc cccggacgag gccaaggcca gtaaaccccg tgtaattgcc tgagcggctt
     1321 cctgcttcgt ttccggcagc gcggacggcg tgaccagcgc ccgccggttt tcaggcgcgt
     1381 tcgggtcatg cagccccagc ctgccgttca cgatggtctg ccaggcatca atgcgcggac
     1441 ggtcggcgcg gtcgtaatac ggctggagac gcttgccagt cagcagctcc gtgttcggga
     1501 tcaggaagtt cagctccagc cgccccttat cctggtgctc aacccacagc acgctgtact
     1561 ggtctttgtc gagtccgggc atcagaaccc gttcaaagct cgccatcagc ttttcgcgct
     1621 gcccgggcgg taaatccttt tcggcaaagg acaggacgcc ggatgtgtac ttttttgcgt
     1681 agggtgaggc gtcgataagc tccctgactt cttccggctt cccctgcaga acggtggcgc
     1741 cctcgcgctg tcggtctttc cccagcagat aatccaccgg accggcgcca ccgccgcgcc
     1801 ctcgcgggtg aaacttaacg atcatcgtct gcaccctttt ccagcacggc atgccgcagc
     1861 cgctcgagtc cggcatctat ggccatcagc gcggcgacga cctgcacgcg gtcgtgcccg
     1921 ctgccaccac cggcgttaac ctgccgggca atctggttaa ggttgttccc catgccggca
     1981 agctgacgaa gcagcgccgg cgagatcgag ggaagcttgc cggcgcgggc gggtttctcg
     2041 tccaggcacg tctggcgcat ccaggctgca agctgtttac cgtcgcagcg ctccagcaga
     2101 cgccggtgct catcctcggt cacccacatc gtgagcattt tgctgcgttt gtctgccaat
     2161 ccggcctcct gataacgccg ggacgggcgg gaaatctttt cccggtcggc gcggtgttgg
     2221 acaagccgca ggcgcgtcag tggttttagc gggtttcggg gcgcagccct gaaccagtca
     2281 cgtagcgcta gcggagtgta tactggctta atatggtaaa tcgaagtggg aattcaggga
     2341 agtgcttcat gtggcaggta aaaaatgcgt ctgtggggcg taagtgcgaa atgctgggga
     2401 cgatgaaatg acgcctcctc gctcactcgg tcgctacgct cctgccgtga gactgcggcg
     2461 ggcgttaccg gctcacgaat ggcgggataa agagatggcg ctcaaatcag gaagggctgg
     2521 gaaaaggatg cggcgttgcc gtttttccat aggctccgcc cccctgacaa gcatcacgaa
     2581 atatgacgct caaatcagtg gcggcgaaac ccgacaggac tataaagatc ccaggcgttt
     2641 ccccctggta gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt
     2701 ccgctgttat gtggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca
     2761 gttcgctcca agctggactg tatgcacgac ccccccgttc agtccgacta ccacgcccat
     2821 tccggtaact atcaacttga gtccaacccg gaaagacacg acaaaatcgc cattggcggt
     2881 agccactggt aagcaggatg tgcgagagat gtatcgagag ttcttgaagt ggtggccgga
     2941 ctacggctac actggaagga cagatttggt gacacgtctc gcacaagact gttaccacgg
     3001 ttcagaagtt ccccaactta ctgaaccttc gaaaaaccac ctccccaggt ggttttttcg
     3061 ttttcagagc aagagattac gcgcagagca aaacgatctc aagaagatca ttttacaaat
     3121 tggataatat ccctccgccc ctaacactaa tataaaatta tcgggcttgc atgcccctca
     3181 ccattttccc catatccacc accaaacaat agttcgtcga gttctttgct agcttgatca
     3241 atacacgcag ccacatgaat agcagaaggg tactcatcat actgaaaatt atcgcttttt
     3301 tcaggaggga gtcgatcata atacggatga caatccacat aataatgacg tattagcatc
     3361 ccagttttat cttcgtactc tgatggctgc aacgtactcc ctgggaaagc ttttgcaaca
     3421 tgtatagcag cttgaaaggt atcaaaatat cgcccaaaca ttggcgtttc aaagctaggc
     3481 gactctgtcg aaaaatcccc cataacttgc gccaaataac cattatctat ctgccattga
     3541 acatctgatc ccatagccca ctctcccaga gttactccta ataaataacc ataatatgta
     3601 tttaccataa cgcttcttgg ccgcaccccc tgagagcccc tcagcgcgtt tctgcggcct
     3661 gagggccgtt tccggcacct ttccccggcc caaaccctga aaaacgcccc tgataatttc
     3721 tgcgcggctg catggctatg cacaagagtg aatggcggcc ggtttcgtga aggaaatacc
     3781 gtgaaagctc cgaaagaaac gcttaattgc acagcagcac atgaacgtga tcagatgaaa
     3841 tctgtctact gccgcacttc ttcacgaaag gcccgctgag cgtcagattc gggcctttct
     3901 tactaaaaag atttcaatag aattttttag caggcgattt ttatctataa ccatacattg
     3961 agcatagaag acccaaattt atgtatgact caatagaaaa aaacaaatca cagccatcta
     4021 aacactgaga cagcaagcca aacaaaaatc aaagaaataa ctccattttt aatcaaataa
     4081 tatagttttc gattcctttt ttttagcttc tgacctgcat cacgaatagc attaacatat
     4141 ttaaataaat gatttatagc accagtcctg tcattattat cgttgggtga tgctgccgct
     4201 gacattagca ccccacctat ccacctattt attttttgtg accaccgcca cttcatcggc
     4261 ctttctatat cacagaacaa aataatacgt gtctgctcgg tatcattctg agcccagtga
     4321 acaaaagttt cgtcaaaaat tatcacttca ccatcatgcc aactgcggcg tacaccatca
     4381 acttcaataa aacaccgttc atcatttggt gttgataatc ctaaatgata acgaactgaa
     4441 ccggcataag ggtcacgatg ctttcctaaa tggcttcctg aaggcaattc agcaaacatc
     4501 gctgctttaa tacagggaat agagctaacc agttgggttg ttactgggca aagccgctcc
     4561 gctgatggat gagcatctcc ataccacttg aggtaaaagc gcttccaacc gcgcttaaaa
     4621 aatgtattaa atcctgcatc attatttttt tcagatgatt taatgtgccc ttgcaaatta
     4681 acagcttcgt ctcttataac ctgccagtta tcaattaatg gttgaagttc aggaaatgtt
     4741 tctgggggaa accatgcctg
//
