LOCUS       NZ_CP147636             3874 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain GN03826 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP147636
VERSION     NZ_CP147636.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40123789
            Assembly: GCF_037289385.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Parsons,J.B., Sidders,A.E., Velez,A.Z., Hanson,B.M.,
            Angeles-Solano,M., Ruffin,F., Rowe,S.E., Arias,C.A., Fowler,V.G.
            Jr., Thaden,J.T. and Conlon,B.P.
  TITLE     In-patient evolution of a high-persister Escherichia coli strain
            with reduced in vivo antibiotic susceptibility
  JOURNAL   Proc Natl Acad Sci U S A 121 (3), e2314514121 (2024)
   PUBMED   38190524
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Hanson,B.M., Parsons,J.B. and Conlon,B.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2024) Center for Antimicrobial Resistance and
            Microbial Genomics, The University of Texas Health Sciences Center,
            1200 Pressler St, Houston, TX 77030, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: NOV-2021
            Assembly Method                   :: Flye v. v2.8.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina NextSeq and Oxford Nanopore
            GridION X5
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037289385.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:16:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,800
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,511
            CDSs (with protein)               :: 5,511
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 77 of 162
            Pseudo Genes (incomplete)         :: 98 of 162
            Pseudo Genes (internal stop)      :: 32 of 162
            Pseudo Genes (multiple problems)  :: 35 of 162
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147636.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="GN03826"
                     /isolation_source="bloodstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA: Durham"
                     /lat_lon="36.0070 N 78.9371 W"
                     /collected_by="Duke Bloodstream Infection Biorepository"
     gene            join(3749..3874,1..813)
                     /locus_tag="WCU51_RS28985"
                     /old_locus_tag="WCU51_28980"
     CDS             join(3749..3874,1..813)
                     /locus_tag="WCU51_RS28985"
                     /old_locus_tag="WCU51_28980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007888271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GT-D fold domain-containing glycosyltransferase"
                     /protein_id="WP_094354373.1"
                     /translation="MLNKNTIYRKVRFPFKIIHALIKYPMANLKAKKYTVLTLEETMDL
                     LLNNDNLSISRYGDGELGMTTFNNIGFQSFDENLSIRLKEIMQEGIKNKNCLICMPDAF
                     RGTKNMRFGPSLFWFFHKAFRFKSYEHLLNDKYQYGNTSITRPYIDYKEKKQSKVIFEK
                     FKQIFKDKKILIVEGAGTRLGRGNDLLANALEIKRITTINKNAFSLYTVLYRTVLDHAK
                     DFDLVLISLGPTATVLSYDISKHGIRCIDTGHIDIEYEWMCSSAQNKVAVNRKNVNEAG
                     VLLSDEDKETDDDYQRQVIMHIGIKNQPQKG"
                     /name="GT-D fold domain-containing glycosyltransferase"
     gene            complement(871..>1142)
                     /locus_tag="WCU51_RS28990"
                     /old_locus_tag="WCU51_28985"
                     /pseudo=""
     CDS             complement(871..>1142)
                     /locus_tag="WCU51_RS28990"
                     /old_locus_tag="WCU51_28985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /name="IS5/IS1182 family transposase"
     gene            1850..2107
                     /locus_tag="WCU51_RS28995"
                     /old_locus_tag="WCU51_28990"
     CDS             1850..2107
                     /locus_tag="WCU51_RS28995"
                     /old_locus_tag="WCU51_28990"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_185773772.1"
                     /translation="MNCIREGLTIWHLQNIITNKKNKIGKNFSISANVIIGHAKGKTPE
                     IGDNVILMVNTSVLGAKICNNVAIGANTLVIKDISEERSV"
                     /name="hypothetical protein"
     gene            complement(2187..3095)
                     /locus_tag="WCU51_RS29000"
                     /old_locus_tag="WCU51_28995"
     CDS             complement(2187..3095)
                     /locus_tag="WCU51_RS29000"
                     /old_locus_tag="WCU51_28995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_077270086.1"
                     /translation="MHNAALELFNDRLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRVGAAIDWSDRNAPAPTLTITNPENGHAHLLYALETSIRTAPDGKMK
                     PLRYAAAVENALRKKLGADTGYSGLICKNPNHSHWKIAVWQPELYTLDWLADSLDLSAA
                     NDKEIVSDYGLGRNCTLFDRTRKWAYRAIRQGWPEYGQWLQACYERASAYNLQFAAPLD
                     ENEVAGIAQSIAKWTSKNFNKDIFSEKQKIKGSKGGRSRSMTYESRRKIARILSDDGFS
                     IKEIKEIIGTPLSTLYRWLST"
                     /name="replication initiation protein"
ORIGIN
        1 atggaccttt tactaaataa tgacaacctg tctattagcc gttatggtga tggtgaactt
       61 ggaatgacga catttaataa cataggattt caatcatttg atgaaaacct atctattaga
      121 ctgaaggaaa taatgcaaga aggaataaaa aacaagaact gcttaatatg catgcccgat
      181 gcttttcgag ggacaaaaaa catgagattt ggcccatcat tattctggtt ttttcacaag
      241 gcatttcgct ttaaaagtta tgaacactta ttaaatgata aatatcaata tggtaacaca
      301 tcaataacaa gaccatatat tgattataaa gaaaaaaaac aatccaaggt tattttcgag
      361 aaattcaaac agatatttaa agacaaaaaa atattaatcg ttgaaggtgc aggaacacgc
      421 ttaggtagag gcaatgactt actagccaat gcactagaaa taaaacgtat aacaacgata
      481 aacaaaaatg ctttttcact atacactgtt ctttatagaa cagtgctgga ccatgctaaa
      541 gattttgacc ttgtgcttat ttcactggga cctacggcaa ctgtgctatc ttatgatata
      601 agcaagcatg gaattagatg catcgataca ggccatatcg atattgagta tgaatggatg
      661 tgtagttctg ctcaaaataa ggtagcagtt aatagaaaaa acgttaacga agctggtgtc
      721 ttacttagtg atgaagataa agaaactgat gatgattacc agcgtcaagt tattatgcat
      781 ataggtatta aaaaccaacc ccaaaagggg taatgggctt tattgcataa atcggatgtt
      841 gggcagatat ccctgtctct gagcgggttt tcaagcaata cgtacgcttt ccggcatacc
      901 ctctttcgtc attttgttca gtgcacacac gatgtgccag agcctacgct acctggccat
      961 cgtaatcacg taacgtcagt gaaccgccga acaactgttt tatccgatac atcgccgttt
     1021 ctgctatcga acgccggttg taatctgttg cgcattactt ccgctcagac gctgattcgc
     1081 tactgcccgg tacggtctgc gtacccgcct gcgccacgtc gtggcggtat cagtgcactg
     1141 acccactatc aaatcgccaa aaaaagaaca aaccgtaacg gtgaacgggt gggcaagtca
     1201 ttggccaact gtgagacgtt gggcaacgag caacgcgagg cggcagtgtg aacgtgacaa
     1261 catcagcggc agatgaaatg acgcttcctc gctcattcgc tcacttcgtt cggtcatgag
     1321 actgcggcga gcgttgcgtg cgaaagaagt gagaatattc gcgtgttaag tgcgaaaaat
     1381 gcgcgccatt cgcaaggggg tttcggggga tgcaatcccc tgaaccagtc acggtgaacc
     1441 ctcgctgagg ggactctgtg tgtactggct tagggtgaga tgtattacgt taagtaaact
     1501 ggctgaaaga accaccaacc cctaaaaaga gggctgttat ctgatatggc tactgagaaa
     1561 atgatatacg cagtgctgaa atagaaaata aaatgggatc atatatgtta gaaacaaaaa
     1621 atagagtatt atcaaagcaa gattcaaata gatttatgga gtgcgacagg atagctctag
     1681 gggtaaaaac aaaaaaacct agcttcctaa gggattacac atggagattt taataaaatt
     1741 aagaaaaaca gaatactgat ctaataaaaa atgggcggtg agaatatggt tcaaggtaag
     1801 aattattgat tacagaagaa cttgccgggt gaattgacta gaaataccat tgaactgtat
     1861 tagagaggga ctaaccatat ggcacttgca aaatataata acaaataaaa aaaacaaaat
     1921 aggtaaaaac ttttccattt cagccaatgt tataattggg catgcaaaag gtaaaacacc
     1981 cgaaattggt gataatgtaa tattaatggt aaacacatca gtactcggtg caaaaatatg
     2041 caacaacgtt gctattggtg caaatacact tgtaattaaa gatatttcag aagaaagatc
     2101 tgtttaggct ggttacccgg caaaaaagat atcaaataac cccccttcac cacataaaga
     2161 caaggggatt gaaatagcaa aaatcgctaa gtagataacc atctatataa tgttgataat
     2221 ggggtgccga tgatttcttt tatttctttt atagaaaatc catcatcgga caaaatacgc
     2281 gcaatttttc tacgtgactc ataagtcata gacctgctac gccccccttt acttcctttt
     2341 attttttgtt tttctgaaaa aatatcctta ttgaaattct tgctggtcca tttcgcaatg
     2401 ctttgggcta tcccggccac ctcattttca tccagaggcg ctgcaaattg cagattatag
     2461 gcgctggcac gttcgtagca ggcttgcagc cactggccat actcaggcca gccctgacgg
     2521 atagcgcgat atgcccactt gcgggttctg tcgaacagag tgcagttacg gcctaagccg
     2581 tagtcggaga cgatttcttt gtcgttcgcc gcgctcaggt caagcgagtc ggccagccag
     2641 tccagcgtgt agagttccgg ctgccatacg gcaattttcc agtgactgtg gttcggattc
     2701 ttgcagatca accccgaata gcctgtgtca gcgcccaatt ttttacgtag cgcgttctct
     2761 acagcagctg cgtagcgtaa tggcttcatt tttccgtctg gcgcagtacg tatcgaggtt
     2821 tccagcgcgt aaagcaggtg cgcgtgtccg ttttctggat tagtgatggt cagcgtaggt
     2881 gcgggggcgt tgcggtcgct ccagtcaatg gcagcaccga cgcgatcgac gtcaaatccc
     2941 agccagaaca tggcgtgggg ctgattgaac tggatgtatt tcgccagaat agcgcgctct
     3001 ttccctgcga tgcgtacacc aaagtgcaga tcatccgaga agtacggctt atgcggtaag
     3061 cggtcgttaa aaagttcgag tgcggcgtta tgcaatgtgc cctgcgctta tggcgggctg
     3121 ttgtctggca cggttcatgt gctaatatcc tttcaaggtt tcgacctggc cctggttatc
     3181 atgcgatccg ccaagatatg acatgatttc cggggcttag ttttttctgg gcttaaagtt
     3241 acaccgtctt gcgcgatctg tcgatctcac cgcgatttac cataacatcc gttatcagca
     3301 ccgcctcaga cgctctcagc acgtttctgc gccctcatcc acaaaagaac atgtcttacc
     3361 ccctgaaaaa cgctcagaat ggctttggtg atttctgacg aggtgaatcg ttatgcacct
     3421 agctgcctag agactgtttt cgggtcaaaa tctccgtgta agccacaaaa cccgaacaaa
     3481 ccggtaacgg tgaacgggtg ggcaattcat tgacaagcta tgaggcatta ggcaaagctc
     3541 agagcgaggc gggagtgtga acacagcaac atcagcttca gatgaaatga ctcgtcctcg
     3601 ctcactacgt taggtactga gactgtggcg tgcgtggaac aggatgaaga aagcaggcct
     3661 aactgtaggc gtttgttata gttatcaata tgctagtgaa atgattactc cacgagataa
     3721 acttaatcag taaggtaaat aattaggtat gctaaataaa aatacaatat atagaaaggt
     3781 tcgcttccca ttcaagatca tacatgcatt aattaaatat ccaatggcaa atttaaaagc
     3841 caaaaaatac acagtactta ctctagagga gacc
//
