LOCUS       NZ_CP147631             5952 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain GN03826 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP147631
VERSION     NZ_CP147631.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40123789
            Assembly: GCF_037289385.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Parsons,J.B., Sidders,A.E., Velez,A.Z., Hanson,B.M.,
            Angeles-Solano,M., Ruffin,F., Rowe,S.E., Arias,C.A., Fowler,V.G.
            Jr., Thaden,J.T. and Conlon,B.P.
  TITLE     In-patient evolution of a high-persister Escherichia coli strain
            with reduced in vivo antibiotic susceptibility
  JOURNAL   Proc Natl Acad Sci U S A 121 (3), e2314514121 (2024)
   PUBMED   38190524
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   Hanson,B.M., Parsons,J.B. and Conlon,B.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2024) Center for Antimicrobial Resistance and
            Microbial Genomics, The University of Texas Health Sciences Center,
            1200 Pressler St, Houston, TX 77030, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: NOV-2021
            Assembly Method                   :: Flye v. v2.8.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina NextSeq and Oxford Nanopore
            GridION X5
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037289385.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:16:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,800
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,511
            CDSs (with protein)               :: 5,511
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 77 of 162
            Pseudo Genes (incomplete)         :: 98 of 162
            Pseudo Genes (internal stop)      :: 32 of 162
            Pseudo Genes (multiple problems)  :: 35 of 162
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147631.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="GN03826"
                     /isolation_source="bloodstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA: Durham"
                     /lat_lon="36.0070 N 78.9371 W"
                     /collected_by="Duke Bloodstream Infection Biorepository"
     gene            1..2301
                     /gene="kba"
                     /locus_tag="WCU51_RS00005"
                     /old_locus_tag="WCU51_00005"
     CDS             1..2301
                     /gene="kba"
                     /locus_tag="WCU51_RS00005"
                     /old_locus_tag="WCU51_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease bacteriocin klebicin B"
                     /protein_id="WP_267723595.1"
                     /translation="MGGGFNYNGEGATGTGLDRDPYVRDSNGNAIGVKSGYHAESYGTS
                     SPALGPNGAIQITAGVIAVPGDKPRPDGGSGGGNTVNTGPAGQLLVMNKGQLGYWETRS
                     TGAGNNEHNTSVFVAVGPSEAEKTASAEKALKEKQQAEAAAKDFAAKTAAASATAEKER
                     QQAIAAATAAGQHQSVSDARNSLNNATSDVSRLKSAADSALQEAKAKRKAAIDAVPVAT
                     QAENKYQELQQKIKGLKLKNGEYGTDKWEIIGSNKEHDHWGYRFYPSGITKAQVDAAQS
                     DAVNKRNQATSLASQATAAEQDSLKATAAYNAAETRRQAAQAALNSAEQAAAAAERKRQ
                     EAEAAAAAAAEKKRQADAAAKAAEEARAAAEKARLMQERQAAADKLKSTDIQSVRGIPS
                     TAAPAASPISWAVASLGGISLDSVTAGKAWTQIAEVMAKLRGIAGASLVGPVVATAVGL
                     FWSRDVGIGSDVVPGRDISGLMPGDALSLPDLATLIKAADSKTGVSMPVRGRIIVREGD
                     YLESQFVRTPVAGSVPVVRAALDKATGYWGYTLPAIQGVPGQTILVSPSDAPGVNGPLG
                     LAGPVPLPETIIHTGGQTTVPQGGTVTVSPAEDDIDFNDLILVFPPESGLKPLYVMYRS
                     PRNMPGTASGKGQNVGNNWMGGASTGDGAPVPSQIADKLRGKAFGSFDSFRRAFWKAVA
                     ADPDLSKQFYPDDIERMKLGRAPTVRFRDSVGKRVKVELHHKVEISKGGDVYNVDNLNA
                     LTPKRHIEIHKGN"
                     /name="kba"
     gene            2303..2560
                     /locus_tag="WCU51_RS00010"
                     /old_locus_tag="WCU51_00010"
     CDS             2303..2560
                     /locus_tag="WCU51_RS00010"
                     /old_locus_tag="WCU51_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015366249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_010904244.1"
                     /translation="MANKTLADYTEQEFIEFIEKIKKADFATESEHDEAIYEFSQLTEH
                     PDGWDLIYHPQAGADNSPAGVVKTVKEWRAANGKPGFKKS"
                     /name="bacteriocin immunity protein"
     gene            2668..2823
                     /locus_tag="WCU51_RS00015"
                     /old_locus_tag="WCU51_00015"
     CDS             2668..2823
                     /locus_tag="WCU51_RS00015"
                     /old_locus_tag="WCU51_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323621.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071561715.1"
                     /translation="MKKLLFLGVVTVSVVLAACQVNNVRDTGGGAVSPSSTVTGVTLDS
                     GTNGNP"
                     /name="colicin release lysis protein"
     gene            complement(3028..3141)
                     /locus_tag="WCU51_RS00020"
                     /old_locus_tag="WCU51_00020"
     CDS             complement(3028..3141)
                     /locus_tag="WCU51_RS00020"
                     /old_locus_tag="WCU51_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_191720660.1"
                     /translation="MHEPPVQSDRCAFSGNYHLESNPERHDKTPLAAATGN"
                     /name="replication initiation protein"
     gene            3560..3751
                     /locus_tag="WCU51_RS00025"
                     /old_locus_tag="WCU51_00025"
     CDS             3560..3751
                     /locus_tag="WCU51_RS00025"
                     /old_locus_tag="WCU51_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4222..4413
                     /locus_tag="WCU51_RS00030"
                     /old_locus_tag="WCU51_00030"
     CDS             4222..4413
                     /locus_tag="WCU51_RS00030"
                     /old_locus_tag="WCU51_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032421723.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4413..4835
                     /locus_tag="WCU51_RS00035"
                     /old_locus_tag="WCU51_00035"
     CDS             4413..4835
                     /locus_tag="WCU51_RS00035"
                     /old_locus_tag="WCU51_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_338850105.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGVNSVGLHDLQHRIALLEAENDGLKRAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(3062..3685)
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.151"
                     /coverage="99.0"
                     /evalue="1.57e-153"
ORIGIN
        1 atgggtggtg gatttaacta taacggggaa ggtgctactg gcaccggatt ggatcgtgat
       61 ccatatgttc gcgacagcaa tggtaatgct attggtgtta aatcaggcta tcacgcggag
      121 tcctatggga cgtcaagtcc agccttaggg cctaatggcg ctattcagat tactgctggg
      181 gttattgctg tgcctggaga taagccccga cctgacggtg gtagtggtgg tgggaatact
      241 gttaacaccg gacctgcagg acagctcctg gtgatgaata aaggtcagct tggatactgg
      301 gaaactcgtt ctacgggcgc gggtaacaat gagcataata cgagtgtatt tgttgctgta
      361 ggtccttctg aggcagaaaa aactgcttct gcagagaagg cgttaaagga aaaacagcag
      421 gcagaagcag cagcaaaaga ctttgcggct aaaactgctg ccgcatcagc cacggcggaa
      481 aaagaacgcc agcaggcaat tgctgctgca actgcagcgg gtcagcatca gtcagtttct
      541 gatgcccgta atagcctgaa taacgcgacg tcagatgtgt ctcgtctgaa atcagctgca
      601 gacagtgcac tgcaggaggc aaaggcaaaa cggaaggctg ctattgatgc tgtacctgtt
      661 gcaacccagg cggaaaataa atatcaggag ctgcagcaga agattaaagg cctgaagctg
      721 aaaaatggtg agtatggtac ggataaatgg gaaataattg gctctaataa ggagcatgat
      781 cactggggat acaggtttta tccatccgga attaccaaag ctcaggttga tgcggcgcag
      841 agcgatgctg tgaataagcg aaatcaggct accagtcttg ccagtcaggc aacagcagca
      901 gaacaggaca gcctgaaagc tacagctgcc tataatgcgg cagaaacgcg ccgtcaggct
      961 gctcaggcgg cgttaaattc tgctgaacag gctgctgctg ctgctgaacg taagcggcag
     1021 gaagctgagg cggctgcagc agctgctgct gagaaaaaac gtcaggcaga cgcagcagca
     1081 aaagctgcag aggaagcgcg tgcggcagcg gaaaaagcca ggctgatgca ggagcgtcag
     1141 gcagcagcag ataagctgaa atccacagat attcagtctg ttcgcgggat cccgtctacg
     1201 gctgcgcctg cagcgtcacc catttcctgg gccgttgcat cacttggtgg tatatcgctg
     1261 gatagtgtta ctgcagggaa agcctggacg cagattgctg aggtgatggc taaactacga
     1321 ggtattgccg gtgcgagtct tgttggtccc gtggtggcaa ctgctgtagg gctgttttgg
     1381 tcacgtgatg ttggtattgg cagtgatgtg gtgcccggac gtgacatcag cgggctgatg
     1441 ccgggtgatg cactgtcatt acctgatctg gccactttga ttaaagctgc tgacagtaaa
     1501 acgggtgtca gtatgccggt tcgaggccgg attatcgtgc gtgaaggcga ttatctggag
     1561 tctcagttcg ttcgaacacc tgttgccggt agtgttccgg ttgttcgggc tgctctggat
     1621 aaagctactg gttactgggg atatacgttg ccggcgatac agggtgtgcc cggacagaca
     1681 atactggtga gtccgtcaga tgcgccgggc gttaatggtc ctctgggact tgctgggccg
     1741 gttcctttgc ctgaaactat tatacatacc ggtgggcaaa ctacggttcc tcaggggggg
     1801 actgtgacag tttcgccggc agaagacgat attgatttca atgatttgat tctggtattt
     1861 ccgccggagt ccggtcttaa accgttgtat gtgatgtacc gtagccctcg taacatgccg
     1921 gggacagcca gtggtaaagg tcagaacgtt ggaaataact ggatgggggg ggccagtacc
     1981 ggggatggtg ctcctgttcc ttcccagatt gcagataaat tacgtgggaa ggctttcggt
     2041 agttttgatt ctttccgtcg ggctttctgg aaagcggttg ctgctgatcc ggacctcagt
     2101 aagcagtttt atcctgatga tatagaacga atgaaattag ggcgagctcc aacagttcga
     2161 ttccgagatt ctgtaggtaa aagggttaag gttgaactac accataaagt tgaaatttct
     2221 aaaggtggcg atgtctataa cgtagataac ctgaatgcat taacacctaa acgtcatatt
     2281 gaaattcata aggggaactg aaatggctaa taaaactttg gctgactata cagagcagga
     2341 atttattgag tttatcgaaa aaattaaaaa ggcagacttt gctactgagt ctgagcatga
     2401 tgaggctatc tatgagttca gccagttgac tgaacatcca gatggttggg atcttattta
     2461 tcatcctcaa gcaggagccg ataactctcc tgctggtgtt gtaaaaacag taaaagagtg
     2521 gcgagcagct aacggtaagc caggttttaa aaaatcgtga ttgaatgtgc gggttcaagt
     2581 gatgaatccg cacattttta actctggatt tgaataataa ttactagata tccatttgcc
     2641 tagaataata aaaataaaag gtgatatatg aaaaaattac ttttcctggg ggttgttacc
     2701 gtttctgtgg ttctggcggc atgtcaggtc aataatgttc gtgacaccgg aggtggggct
     2761 gtttcacctt catcgacggt gaccggagtc acgcttgata gcggtacgaa cggcaatccg
     2821 taaaggatct tcttgagatc ccatttggat cgtcgtaatc tcttgctctg taaacgaaaa
     2881 aaccgccttg gcgggcggtt ttttcgaagg ttcgaggagt tggcgctctt tgaaccgagg
     2941 taactggctt ggaggagcgc agtaaccaaa tttgttcttt cagtttagcc ttaactggca
     3001 cataacttca agactaactc ctctaaatca gttaccagtg gctgctgcca gtggcgtttt
     3061 gtcgtgcctc tccgggttgg actcaagatg atagttaccg gagaaggcgc agcggtcgga
     3121 ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgccttcc cggaactgag
     3181 tgtcaggcgt ggaatgagat aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa
     3241 tggcaggaac aggagagcgc gcgagggagc taccaggggg aaacacctgg gatctttata
     3301 gtcctgtcgg gtttcgccac cgctgatttg agcgtcagat tttatgatac tcgtcagggg
     3361 ggcggagcct atggaaaaac gccccggcgg ggcgtggcct tttcgccctt gtccttagct
     3421 ttcgttatgc ttaccgttac gttatctgct tatcttccct ccgctcgccg cagactcatg
     3481 accgaacgga gtgagcgaat gagcgaggaa gcggaaaacc atcagaacgt acagtaagca
     3541 ctttcgcgcc ggggccttca tgaacaaaca acaaaagact gccgttaaca tggctaaatt
     3601 catccaggat cagtccctgc ttcttctgga caggcttaat gagctggatc tggatcatga
     3661 ggctgatttc tgcgagaaac tccatgagga tgcggaacgc ctttaccgaa gcctgtcagc
     3721 gcgcctgaat gagcagcagg acggtgcgta atggctccgt tctgggtatt accggagtaa
     3781 taccggcatc gccttaattt tcattgtgtt agcgcttaac attattaccc cggtaatacc
     3841 cagaatccct gtattttttc ttttttattt atttttcatg aagttgcgat gaaaatcgcg
     3901 gctgcgttag gtgtatgacc attttgaggt tgaaaaataa tcaaccatgg cgggtgattt
     3961 acgcaggtta aggggcgtcg gtgacaaaaa aacgtcatta acgacctcgt tcatgctcgc
     4021 atgaacggat tatttttccg gactacaggt aaactacaga aagactacaa aagctgatga
     4081 cttaatcctg atttttagtg cgatgactac agataaacta caaaataact acaggattac
     4141 ctcagatccg ttctctgaac cattcgtaca tccgaccccc ttaccggttt tacactgagc
     4201 actgatgctc tgaggtccct tatgacgaaa caacagaaaa cagcgctgaa catggctaaa
     4261 ttcatccaga atcagtctct gctcctgctg gagaagctta acgagcttga tctcgatgct
     4321 gaggccgacc tttgtgagaa actccacgat gatgcggagc atcttttccg tacactgtcc
     4381 tcgagactgg atgaattaca ggatggtaac tgatggccag agtgtctatt agtgaagcag
     4441 cacggcttgt taaggttagt cgccctacta tttacaagat gattaacagt ggtaagttaa
     4501 gttacacctc tgttgtaaag cacgggaagg ctattaaagt tattgatacc tcagagctta
     4561 ttagagtgtt tggttctctt gatggtgtca tcgatactgt aaaatatgat gtaaaaagtg
     4621 acgcagaatc tacaggtgtt aacagtgttg gtttacatga tttacaacat agaattgctt
     4681 tactagaagc tgaaaacgat ggtcttaaaa gggctgttaa ggctcgagat gagcatattg
     4741 attcccttcg tcaggctatg cagcttctgg agcacaagca cgaaccttct tcaccacccc
     4801 attccccgtg gtggaaattc tggaaaaaat cctgaccccg tcatgcttca gttttcctct
     4861 ccggccatcc cggggagttc ctgaaccttc ccaaaaatcg ccagatctct gaacgctcct
     4921 cacgactaaa aatacccaca cactacgcaa aattaaaatt ttgcagattt gtatttaata
     4981 tcagtggttt actttcgtga aatttcgttt tttaaatttt atttacatag atccgactct
     5041 atgacgactc gtcatagagt ccccggcgag tcgtcataga gtcggtaacg actcaggact
     5101 ataaaaacaa tgagttaata acgacccgac tctagacccg actccccgtt tggttgtttt
     5161 ttagactcgg cgactctggg tacgactcta gtgaagagtc gccggtgaat cgtgtggtga
     5221 gtcgtccggc gacttgccat aacgtgattg ccggccgtcg acgaacacga ttcctgaacg
     5281 tgctgaaaac cggttttccg gctgcgccgg caacgcttca cggccacgca ataaattgcg
     5341 tccccgttca ccgttttcgg atggaaagaa cgccggaaac aggacttacc ctggctgaat
     5401 ggcgtgagtt ttcgcgctat gcacttgtgc gcatactcat gcatgccgta aaaacggagc
     5461 ctgcgcgttt ctggcgggtt ttcgggtggt ttgttgcctg ttttgccggt tttccgtcag
     5521 aaacgccctg aggccgtttt agcggtgcgt acaattaagg gattatggta aatgggagcg
     5581 cccgggtggc gggataccct caacaccaag actttttgta caggtctctg tccgtcagtc
     5641 acaccttttt taagagcaca tatcgttaat tttttttctc tgccgagccg cgtcctgtct
     5701 gggttttttc atttctctca tgattaactt aatgaaaatt aaaaagataa ttgtatctct
     5761 ccgcgtgttg acagagttct catgtcagga gtagtatcca agcactgtat ataaacacat
     5821 gtgtttatat acagtgcttt ggtgtggcag agaactaata acagcctccg gatacaggtt
     5881 cgaaggcgat aaaccgcaca ccagattctg acaagcagga aaacgataaa aataacaaca
     5941 aaggagagag tt
//
