LOCUS       NZ_CP147577             9424 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain GN05476 plasmid unnamed2, complete sequence.
ACCESSION   NZ_CP147577
VERSION     NZ_CP147577.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40123768
            Assembly: GCF_037273335.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Parsons,J.B., Sidders,A.E., Velez,A.Z., Hanson,B.M.,
            Angeles-Solano,M., Ruffin,F., Rowe,S.E., Arias,C.A., Fowler,V.G.
            Jr., Thaden,J.T. and Conlon,B.P.
  TITLE     In-patient evolution of a high-persister Escherichia coli strain
            with reduced in vivo antibiotic susceptibility
  JOURNAL   Proc Natl Acad Sci U S A 121 (3), e2314514121 (2024)
   PUBMED   38190524
REFERENCE   2  (bases 1 to 9424)
  AUTHORS   Hanson,B.M., Parsons,J.B. and Conlon,B.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2024) Center for Antimicrobial Resistance and
            Microbial Genomics, The University of Texas Health Sciences Center,
            1200 Pressler St, Houston, TX 77030, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: NOV-2021
            Assembly Method                   :: Flye v. v2.8.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina NextSeq and Oxford Nanopore
            GridION X5
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037273335.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:14:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,293
            CDSs (total)                      :: 5,170
            Genes (coding)                    :: 4,941
            CDSs (with protein)               :: 4,941
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 89 of 229
            Pseudo Genes (incomplete)         :: 146 of 229
            Pseudo Genes (internal stop)      :: 44 of 229
            Pseudo Genes (multiple problems)  :: 45 of 229
            Pseudo Genes (short protein)      :: 3 of 229
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147577.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="GN05476"
                     /isolation_source="bloodstream"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: Durham"
                     /lat_lon="36.0070 N 78.9371 W"
                     /collected_by="Duke Bloodstream Infection Biorepository"
     gene            1..390
                     /locus_tag="WCU38_RS00040"
                     /old_locus_tag="WCU38_00040"
     CDS             1..390
                     /locus_tag="WCU38_RS00040"
                     /old_locus_tag="WCU38_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000477210.1"
                     /translation="MFKSSVKSITSSTILTLLIGCSSTETNKNIVYKVGGDSIILNKYC
                     ILKMYPSQSEKNKGVIIEVNTDSLCKKKLNEITSKNIGKPLISYFDGKVISQPAIIHSA
                     LNPTHYYQPVNDETTVMEIIKAYSK"
                     /name="hypothetical protein"
     gene            complement(454..738)
                     /locus_tag="WCU38_RS00045"
                     /old_locus_tag="WCU38_00045"
     CDS             complement(454..738)
                     /locus_tag="WCU38_RS00045"
                     /old_locus_tag="WCU38_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Z C-terminal domain-related protein"
                     /protein_id="WP_000625497.1"
                     /translation="MISQNVWAPPFFFGPWVELENHTGDIAYTVYFDTLSNVPGPFIAF
                     IWYAPNGPRTVNVIGPASYQISCKGAGVDKIKFKSVSGTGQIIHVYTTL"
                     /name="colicin Z C-terminal domain-related protein"
     gene            complement(756..953)
                     /locus_tag="WCU38_RS00050"
                     /old_locus_tag="WCU38_00050"
     CDS             complement(756..953)
                     /locus_tag="WCU38_RS00050"
                     /old_locus_tag="WCU38_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001335786.1"
                     /translation="MKEEKVLLHRFLFVVRNKNGCELSCSADLMGTRDDVYKYFSDSVS
                     GLDVELIDVSCESEWEEHSH"
                     /name="hypothetical protein"
     gene            1165..1461
                     /locus_tag="WCU38_RS00055"
                     /old_locus_tag="WCU38_00055"
     CDS             1165..1461
                     /locus_tag="WCU38_RS00055"
                     /old_locus_tag="WCU38_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047647.1"
                     /translation="MSKTKSGRHRLSKTDKRLLAALIAAGYEERTARDLIQKHVYTLTQ
                     ADLRHLVSEISNGVGQSQAYDAIYQARRIRLARKFLNGKKPEEGLNAWKGDLP"
                     /name="hypothetical protein"
     gene            complement(2008..2241)
                     /locus_tag="WCU38_RS00060"
                     /old_locus_tag="WCU38_00060"
     CDS             complement(2008..2241)
                     /locus_tag="WCU38_RS00060"
                     /old_locus_tag="WCU38_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001555310.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2248..2766)
                     /locus_tag="WCU38_RS00065"
                     /old_locus_tag="WCU38_00065"
     CDS             complement(2248..2766)
                     /locus_tag="WCU38_RS00065"
                     /old_locus_tag="WCU38_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_338825528.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETILNDEFRKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSC
                     GTLWYLGGMIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFQVLPYGKSAEVIPHQ
                     GKDWVQLME"
                     /name="MbeB family mobilization protein"
     gene            complement(<2804..3502)
                     /locus_tag="WCU38_RS00070"
                     /old_locus_tag="WCU38_00070"
                     /pseudo=""
     CDS             complement(<2804..3502)
                     /locus_tag="WCU38_RS00070"
                     /old_locus_tag="WCU38_00070"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3444..3767)
                     /locus_tag="WCU38_RS00075"
                     /old_locus_tag="WCU38_00075"
     CDS             complement(3444..3767)
                     /locus_tag="WCU38_RS00075"
                     /old_locus_tag="WCU38_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000956005.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3832..4023
                     /locus_tag="WCU38_RS00080"
                     /old_locus_tag="WCU38_00080"
     CDS             3832..4023
                     /locus_tag="WCU38_RS00080"
                     /old_locus_tag="WCU38_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165988.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDSENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4564..4677
                     /locus_tag="WCU38_RS00085"
                     /old_locus_tag="WCU38_00085"
     CDS             4564..4677
                     /locus_tag="WCU38_RS00085"
                     /old_locus_tag="WCU38_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001427889.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGN"
                     /name="hypothetical protein"
     gene            5735..6008
                     /locus_tag="WCU38_RS00090"
                     /old_locus_tag="WCU38_00090"
                     /pseudo=""
     CDS             5735..6008
                     /locus_tag="WCU38_RS00090"
                     /old_locus_tag="WCU38_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(6160..6393)
                     /locus_tag="WCU38_RS00095"
                     /old_locus_tag="WCU38_00095"
     CDS             complement(6160..6393)
                     /locus_tag="WCU38_RS00095"
                     /old_locus_tag="WCU38_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137437.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(6400..6930)
                     /locus_tag="WCU38_RS00100"
                     /old_locus_tag="WCU38_00100"
     CDS             complement(6400..6930)
                     /locus_tag="WCU38_RS00100"
                     /old_locus_tag="WCU38_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<6968..7666)
                     /locus_tag="WCU38_RS00105"
                     /old_locus_tag="WCU38_00105"
                     /pseudo=""
     CDS             complement(<6968..7666)
                     /locus_tag="WCU38_RS00105"
                     /old_locus_tag="WCU38_00105"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(7608..7931)
                     /locus_tag="WCU38_RS00110"
                     /old_locus_tag="WCU38_00110"
     CDS             complement(7608..7931)
                     /locus_tag="WCU38_RS00110"
                     /old_locus_tag="WCU38_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7996..8187
                     /locus_tag="WCU38_RS00115"
                     /old_locus_tag="WCU38_00115"
     CDS             7996..8187
                     /locus_tag="WCU38_RS00115"
                     /old_locus_tag="WCU38_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165975.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8895..9008
                     /locus_tag="WCU38_RS00120"
                     /old_locus_tag="WCU38_00120"
     CDS             8895..9008
                     /locus_tag="WCU38_RS00120"
                     /old_locus_tag="WCU38_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001427889.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGN"
                     /name="hypothetical protein"
     Typing          complement(4109..4196)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(8282..8363)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.795"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          4234..4636
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8374..8643
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.98e-139"
     Typing          8689..9004
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.06"
                     /coverage="100.0"
                     /evalue="6.26e-126"
     Typing          complement(1905..3454)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.555"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6057..7618)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.424"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgttcaaat catcagtaaa gtcaataaca tcgagtacta tactgacctt acttatagga
       61 tgttcaagta ctgaaacaaa caaaaacatt gtttacaaag ttggtggtga cagcatcatt
      121 ctgaataaat attgcatact taaaatgtac ccttcccaat cagaaaaaaa caaaggcgtc
      181 ataatagaag taaacacaga tagtttatgt aagaaaaaac taaacgaaat aactagtaaa
      241 aatataggga aacccctgat ttcatatttt gatggaaaag taatatccca acctgcaatc
      301 attcattctg cactaaatcc aacccattat tatcaacctg tgaacgatga aacaacagtg
      361 atggaaatca taaaagcata ttcaaaataa tattaataga tataatcctg cttaaggtat
      421 agcttaggca ggattatatt tattcagctc ttattataag gtagtgtata catgaataat
      481 ctgccctgtc ccactgacac ttttaaattt aattttatca acaccagcgc ccttgcaaga
      541 gatctgataa gatgctggac caataacgtt aactgttctt ggcccattag gggcatacca
      601 gataaaagca ataaatggac cgggtacatt agacaatgta tcaaaataga cagtgtacgc
      661 gatatcacca gtatgatttt caagttcaac ccaaggacca aaaaagaaag gaggagccca
      721 aacattttga gaaatcatat aatctcctga ttagttcaat gcgaatgttc ttcccattca
      781 ctctcgcaag aaacatctat aagctcaaca tctagcccac taacagaatc agagaaatac
      841 ttataaacat catcccttgt tcccataagg tctgccgaac atgatagttc acaaccattc
      901 ttattcctaa ctacaaataa gaaacggtga agcaatactt tttcttcttt cattttatcc
      961 ctccctttat ttacacagaa tatactgtat atataaatac tagttttata tacagtataa
     1021 attacgccgc attatttttc ctgtcaatga tatgaatttt catttttgtg atctgaatca
     1081 cccttataaa aatcaggaag ggaagattcg cagcagaaaa acagcaccgg ataacatcag
     1141 gagaaaaaac agagaggaga taacgtgagc aaaacaaaat ctggtcgcca ccgactgagc
     1201 aaaacagaca aacgcctgct ggctgcactt atcgctgccg gatacgaaga gcggacagcc
     1261 cgtgacctca tccagaaaca cgtttacaca ctgacacagg ccgacctgcg ccatctggtc
     1321 agtgaaatca gtaacggtgt gggacagtca caggcctacg atgcgattta ccaggcaaga
     1381 cgcattcgtc tcgcccgtaa attcctgaac ggaaaaaaac cggaagaagg gctgaacgcc
     1441 tggaagggtg atttaccata atcacttatt gtacgcaccg ccgaaacgcg ttcggtgcga
     1501 tcacggcagc agacaggtaa aaatggcaac aaaccacccg gaaaactgcc gcgatcgcac
     1561 ccggtaaatt ttaaccacat gcatacctat gcagccaggt gaatcacgca ggaattgcca
     1621 gtcagagacc gctgaaacgg atttttttca tactttcgga agcgaagaag acggggattt
     1681 gagcaggaaa acagaagttc aggggaatga gcgcgatctg gcaatagagg caaaaacacg
     1741 actatgaaag acacaccaga atcgcgcccg tatgcgtttt aacgcgttcc tgtgttttca
     1801 gcaaggttta tgactgaagc gcgttcccgg tgccccgaac caaccattat cccaaaaaat
     1861 gcaccggaaa cgcgctgaca acattttagc ctgtctctga ttaccatccc agcgaagggc
     1921 catccagtgt ccgttcctgt atttccagct ggcgctcacg ctccagggat aactcatgtt
     1981 cgcgttcctg tatcagccgt tcgtccctta tctcctgttc tcgctgtata actggctcaa
     2041 gcgttctgtc cgctcgctca agtgctgcac ctgctgactc aacttcatga cccgctcgtt
     2101 cagcatcgcg ttgtcccgtt gcgtaagcga aaacattttc tgcaattcca cgaaggcgct
     2161 ctcccattcg ttcagccgct gcatatagtc ctgttgcagt tgctctaatg cgttcagcaa
     2221 atgtctttcc agctccgtca ctctgtgtca ctccatcagt tgtacccagt ccttcccctg
     2281 atggggaatc acctctgctg atttcccgta cggaaggacc tgaaatttcc tgttcccgtc
     2341 ctgtacgaac tccacgcccc atgtcttcgc gttcagtttc tgcaatgtct cttcctgctt
     2401 cctgatatct tccaggttcg cctgtatcat cccgccaaga taccagagcg tcccgcaact
     2461 cacggtaaac aggaaaatga ccatccccag taacatcatg cccgtattcc ctgccagctt
     2521 taacacgtcc ttcctgtgct gcatcatcgc ctctttcacc ccttcccggt gctttttcag
     2581 tgattcctct gtcgaagctg tgaacagggc tatagcgtct ttgattttcg tctcgtttga
     2641 tgtcacagcc ttgcttacag atttttcgag cttcctgaac tcgtcgttca gtattgtctc
     2701 tgtagattcg gctctctctt tcagcttttt ctcgaactcc gcgcccgtct gtaaaagatt
     2761 gctcataaaa tgctcctttc agcctgatat tcttcccacc gttcgggtct gcaatgctga
     2821 tactgcttcg cgtcaccctg accacttcaa gccctgcctc tgtgagcgcc tgaatcacat
     2881 cctgacggcc ttttatctcc ccgacatgat aaagagcgtc tatcccgcgt gtgacgctct
     2941 ctgcaagcgc ctgtttcgtt tccggcaggt tatcagagag agtcagtatc cggcggttct
     3001 ccggggcgtt ggggtcatgc agcccgtaat ggtgattcac cagcgtctgc caggcatcaa
     3061 ttctcggcct gtctgcccgg tcgtagtacg gctggaggcg ttttccggtc tgtagctcca
     3121 tgttcggaat gacaaaattc agctcaagcc gtcccttgtc ctggtgctcc acccacagga
     3181 tgctgtactg atttttttcg agaccgggca tcagtacacg ctcaaagctc gccatcacct
     3241 tttcacgtcc tcccggtggc agctccttct ccgcgaacga cagcacaccg gacgtgtatt
     3301 tcttcgcaaa tggcgtggca tcgatgagtt cccgtacttc ttccggatta ccctgaagca
     3361 ccgttgcccc ttcccggtta cgctcccttc ccagcaggta atcaaccgga ccactgccac
     3421 caccttttcc ccgggcatga aacttaacta tcatcccgcg tcccctgttc cctgacggcc
     3481 agacgcaacc ggcgcagctc atccccgatg gccatcagtg cggccaccac ctgaacccgg
     3541 tcgccggaag accactgtcc gctgttcacc ttacgggctg tctggttcag gttattcccg
     3601 atggctgcca gctgacgcag taacggcggt gccagtgtcg gcagcctccc ggaacgggca
     3661 accggctcac ccaggcagac ccgccgcatc cataccgcca gttgtttacc ctcacagcgt
     3721 tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct ctcgcgtttc
     3781 atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc agtgactaaa
     3841 caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt aacgctgctg
     3901 gagaagctca acgaactgga cgcggatgaa caggcagata tctgtgaatc gcttcacgac
     3961 cacgccgatg agctttaccg cagctgcctc gcgcgcttcg gggatgacag tgaaaacctc
     4021 tgacatatgc agctcccgga gacggtcaca gcttgcctgt gagcggatgc cgggagcaga
     4081 caagcccgcc agggcgcgtc agggtgtttt agcgggtttc ggggcgcagc cctgaaccag
     4141 tcacgtagcg atagcggagt gtatactggc ttaaccatgc ggcatcagtg cggattgtat
     4201 gaaacgtgcg ccataccggg tgtgaaatgc cgcacagaat caggggataa caccggaaaa
     4261 aacatgtgag caaaagacca ggaacaggaa gaaggccgcg ccgctggcgt ttttccatag
     4321 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     4381 gacaggactt aaagatacca ggcgtttccc cccggaagct ccctcgtgcg ctctcctgtt
     4441 ccgaccttac cgtttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4501 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4561 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4621 gagtccaacc cggtaagaca cgacttaacg ccactggcag cagccattgg taactaaaaa
     4681 gtggatttag atacgcagaa ctcttgaagt tgaagcctta tagcggctac actgaaagga
     4741 catcttttgg tatctgtgct ccacttaagc cagctacccg gttaggtaat ctgccaggat
     4801 tagctaacct tcgaaaaacc acctgccagg gtggtttttt cgtttacagg cagcagatta
     4861 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt tttttctcta aactgcaatc
     4921 ccatcagttc aggagagaaa gatagtataa ttgccctctc ttatatggaa ccaccattca
     4981 atcacatcat ggggataata taagtgaaaa cgttcgcatt gtttattttt acgtgtatat
     5041 gtatttatag attggaattt taacaaaata aaatggagtt aatatgataa gatgtttgac
     5101 caataacgtt aatcttcgtt cttggcccat taggcatacc agataagtta ataataattt
     5161 tagtattatt agacaatgta tcaaaaatag acagaaaaaa tacgccagat atcaccagta
     5221 tgattttcaa gttcaaccca aggaccaaaa aagaaaggag gagcccaaac attttgagaa
     5281 atcatataat ctcctgatta gttcaatgcg aatgttccca ttcgttcctc cgggtaagaa
     5341 acatctataa gtcaacatct agcccactaa caagaatcag atataaacat catcccttgt
     5401 tcccataggc tgccgaacat gatagttcac aaccatttct attcctaact acaataagaa
     5461 acggtgtcaa tactttttct tctttcattt tatcctccct ttatttacac agaatatact
     5521 gtatatataa atactagttt tatatacagt ataaattacg ccgcattatt tttcctgtca
     5581 atgatatgaa ttttcatttt tgtgatctga atcaccctta taaaaaatca ggaagggaag
     5641 attcgcagca gaaaaacagc accggataac atcaggagaa aaaacagaga ggagataacg
     5701 tgagcaaaac aaaaattcac acagtcgcca ccgactgagc aaaaacagac aaacgtcacc
     5761 acagctggct gcacttatcg ctgccggata cgaagagcgg acagcccgtg acctcatcca
     5821 gaaacacgtt tacacactga cacaggccga cctgcgccat ctggtcagtg aaatcagtaa
     5881 aatcacggtg tggacagtca caggcctact gatgacgcct accaggcaag acgcatcttc
     5941 gtctcgcccg taaattcctg aacgaaaaaa ccggaagaag ggctgaactg gaaagggtga
     6001 ttacttaaac atgaatcaat ttattgtacg caaaccgcga aacgcgttcg gtgcgacatc
     6061 ccagcgaagg gccacccagt gtccgctcct ggatttccgg ctgacgctcc cgctccagag
     6121 ataactcatg ttcgcgttcc tgcatcagcc gttcatccct tatctcctgt tctcgctgta
     6181 taactggctc aagcgttctg tccgctcgct caagtgctgc acctgctgac tcaactgcat
     6241 gactcgcccg ttcagcatcg cgttgtcccg ttgcgtaagc gaaaacattt tctgcaattc
     6301 cacgaaggcg ttctcccatt cgttcagccg ctgcatatag tcctgttgca gttgctctaa
     6361 tgcgttcagc aaatgtcttt ccagctccgt catgctctgt cactcctgca ccagtctcac
     6421 tgcgttcagt ttgccgttgt ccttcgtcca gttcgtttcg gctttcatcc ccttcggcaa
     6481 cactagaaaa cgtccgttgc tgtcctcgtg ataacgcacg ccccatgtct tcgcgttcag
     6541 cttctccaga ctctctttct gctcgcggag aatattcata ttcgcgttta tcttcatgcc
     6601 ctggtaccac agcagactcc cggtcgtggc aaacatcagt actgagacga taatcagcca
     6661 cagccagcgt ttcacgctca ttttcagtac ggttttccag tgcgcgtcca tcgtatcgac
     6721 ggtcgttttc tgatacctct ccagcagttc tttcagggct gttgtatggt cgttgatagc
     6781 gttcctgatt aaagattcgt ttgaggtcag ttccctgttt acagattctt cgagcttcct
     6841 gaactcgtcg ttcagcattt tctctgtaga ttcggctctc tctttcagct ttttctcgaa
     6901 ctccgcgccc gtctgtaaaa gattgctcat aaaacgctcc tttcagtctg atattcctcc
     6961 cgccgttcgg gtctgcaatg ctgatactgc ttcgcgtcac cctgaccact tcaagccccg
     7021 cctctgtgag cgcctgaatc acatcctgac ggccttttat ctctccggca tggtaaagcg
     7081 cgtctatccc gcgtgtgacg ccctcagcaa gcgcctgttt cgtttccggc aggttatcag
     7141 gaagggtcag cgtccggcgg ttctccgggg cgttggggtc atgcagaccg taatggtgat
     7201 tcaccagcgt ctgccaggca tcaattctcg gcctgtctgc ccggtcgtag tacggctgga
     7261 ggcgttttcc ggtctgtagc tccatgttcg gaatgacaaa attcagctca agccgtccct
     7321 tgtcctggtg ctccacccac aggatgctgt actgattttt ctcgagaccg ggcatcagta
     7381 cacgctcaaa gctcgccatc actttttcac gccctccagg cggcagctcc ttctccgcga
     7441 acgacagaac acctgacgtg tatttcttcg caaatggcgt ggcatcgatg agttcccgga
     7501 cctcttccgg attcccctga agcaccgttg ccccttcccg gttacgctcc cttcccagca
     7561 ggtaatcaac cggaccactg ccaccacctt ttcccctggc atgaaactta actatcatcc
     7621 cgcgccccct gttccctgac cgccagacgc agccggcgca gctcatcccc gatggccatc
     7681 agtgcggcca ccacctgaac ccggtcaccg gaagaccact gcccgctgtt caccttcctc
     7741 gctgtctggt taaggttatt cccgatggcg gccagctgac gcagtaacgg cggtgccagt
     7801 gtcggcagct tcccggaacg ggcaaccggc tcacccaggc agacccgccg catccatacc
     7861 gccagttgtt taccctcaca gcgttccagt aaccgggcat gttcatcatc agtaacccgt
     7921 atcgtgagca tcctctcgcg tttcatcggt atcattaccc ccatgaacag aaccccccct
     7981 tacacggagg catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgcttcatc
     8041 agaggccaga cattaacgct gctggagaag ctcaacgaac tggacgcgga tgaacaggca
     8101 gacatctgtg aatcgcttca cgaccacgcc gatgagcttt accgcagttg cctcgcacgc
     8161 ttcggggatg acggtgaaaa ccgctgacac atgcagctcc cggagacggt cacagcttgt
     8221 ctgtgagcgg atgccgggag cagacaagcc cgccagggcg cgtcaggccg ttgacctttg
     8281 gggtgtcggg gcgcagccct gaaccagtca cgtagcgata gcggagtgta tactggctta
     8341 accatgcggc atcagtgcgg attgtatgaa acgtgcgcca taccgggtgt gaaatgccgc
     8401 acagatgcgt aaggagaaaa tgcacgtcca ggcgcttttc cgcttcctcg ctcactgact
     8461 cgctacgctc ggtcgttcgg ctgcggcgag cggtatctac tcactcaaaa acgaaatgct
     8521 gttatccaca gaatcagggg ataacaccgg aaaaaacatg tgagcaaaag accaggaaca
     8581 ggaagaaggc cgcgccgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8641 aaacagacac tttttcgggc atcgtgggac ggctcaaaga aatccgccag aggtggcgaa
     8701 acccgacagg acttaaagat caccagccgt ttcccccggt gaatcgctcc ctcgtgcgcc
     8761 cccctgttcc ggccttaccg tttaccggat acccttgtcc gcctttctcc cttcgggaag
     8821 cgtggcgctt tctcatagct cacgtcttta ggtatctcag ttcggtgtag gccgttcgct
     8881 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8941 actatcgtct tgagtccaac ccggtaagac acgacttaac gccactggca gcagccattg
     9001 gtaactaaaa agtggatgtg agatactcag agactacttg aagttgaagc cttatagcgg
     9061 ctacactgaa aggacatatt ttggtatctg tgctcgcact taagccagct acccggttag
     9121 gtaatctgcc agctgattag ctaaccttcg atcaaaccga cctgccaagg tggttttttc
     9181 gtttacaggc agcagattac gcgcagaaaa aaaggatctc aagaaaggat cctttgatct
     9241 tttttcccta aaacccaaac cccaaaattt aggggaaaaa aaaaaaaaaa acccccctct
     9301 cttatatgga accaccattc aatcacatca tggggataat ataagtgaaa acgttcgcat
     9361 tgtttatttt tacgtgtata tgtatttata gattggaatt ttaacaaaat aaaatggagt
     9421 taat
//
