LOCUS       NZ_CP147507            14517 bp    DNA     circular CON 03-JUN-2024
DEFINITION  Staphylococcus aureus strain BSN139 plasmid pBSN139, complete
            sequence.
ACCESSION   NZ_CP147507
VERSION     NZ_CP147507.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38637718
            Assembly: GCF_037201955.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14517)
  AUTHORS   Berti,A.D., Gurdziel,K. and Liu,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAR-2024) Pharmacy Practice, Wayne State University,
            259 Mack Ave, Detroit, MI 48201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2024
            Assembly Method                   :: SPAdes v. FEB-2024
            Assembly Name                     :: BSN139
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 176.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037201955.1-RS_2024_06_02
            Annotation Date                   :: 06/02/2024 12:46:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,915
            CDSs (total)                      :: 2,833
            Genes (coding)                    :: 2,758
            CDSs (with protein)               :: 2,758
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 42 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 13 of 75
            Pseudo Genes (multiple problems)  :: 16 of 75
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147507.1.
            Bacteria and source DNA available from Andrew D. Berti, Wayne State
            University, Detroit MI  USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..14517
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="BSN139"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pBSN139"
                     /geo_loc_name="USA: Detroit, MI"
                     /lat_lon="42.33 N 83.05 W"
                     /collection_date="2021-10-04"
                     /collected_by="Andrew D. Berti"
                     /genotype="ST8-MSSA CC8"
     gene            complement(join(14475..14517,1..107))
                     /locus_tag="UXR82_RS14495"
                     /old_locus_tag="UXR82_14495"
     CDS             complement(join(14475..14517,1..107))
                     /locus_tag="UXR82_RS14495"
                     /old_locus_tag="UXR82_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000695366.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIVF
                     YEDK"
                     /name="hypothetical protein"
     gene            complement(654..>943)
                     /locus_tag="UXR82_RS14500"
                     /old_locus_tag="UXR82_14500"
                     /pseudo=""
     CDS             complement(654..>943)
                     /locus_tag="UXR82_RS14500"
                     /old_locus_tag="UXR82_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384749.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            complement(963..1946)
                     /locus_tag="UXR82_RS14505"
                     /old_locus_tag="UXR82_14505"
     CDS             complement(963..1946)
                     /locus_tag="UXR82_RS14505"
                     /old_locus_tag="UXR82_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001181107.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRD
                     RLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGLNK
                     PNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPEVRK
                     SNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQEFPE
                     QLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLKKAIHD
                     RGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
                     /name="replication initiator protein A"
     gene            2372..3115
                     /locus_tag="UXR82_RS14510"
                     /old_locus_tag="UXR82_14510"
     CDS             2372..3115
                     /locus_tag="UXR82_RS14510"
                     /old_locus_tag="UXR82_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000843732.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNTR
                     QNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPANLND
                     NDSDIEEKSEETEPKKGFWSRLFGS"
                     /name="DUF536 domain-containing protein"
     gene            complement(3486..4160)
                     /locus_tag="UXR82_RS14515"
                     /old_locus_tag="UXR82_14515"
     CDS             complement(3486..4160)
                     /locus_tag="UXR82_RS14515"
                     /old_locus_tag="UXR82_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="WP_001105991.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDIWL
                     RKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMVKVIKAFKLKPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIML
                     AS"
                     /name="IS6-like element IS257 family transposase"
     gene            4422..4607
                     /locus_tag="UXR82_RS14520"
                     /old_locus_tag="UXR82_14520"
     CDS             4422..4607
                     /locus_tag="UXR82_RS14520"
                     /old_locus_tag="UXR82_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001037130.1"
                     /translation="MNKLEKWSLVFIIFTFLSSLALLYFKYNFSSVMPVLSLSILTILI
                     LLTSIITVVSVNDEDT"
                     /name="hypothetical protein"
     gene            complement(4830..5408)
                     /locus_tag="UXR82_RS14525"
                     /old_locus_tag="UXR82_14525"
     CDS             complement(4830..5408)
                     /locus_tag="UXR82_RS14525"
                     /old_locus_tag="UXR82_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000690637.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNGTPI
                     KTIAEQWQVSRTTIYRYLNKLEAKENKNK"
                     /name="recombinase family protein"
     gene            complement(5672..6052)
                     /gene="blaI"
                     /locus_tag="UXR82_RS14530"
                     /old_locus_tag="UXR82_14530"
     ARG             complement(5676..6052)
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="98.41"
                     /coverage="100.0"
     gene            complement(6042..7799)
                     /gene="blaR1"
                     /locus_tag="UXR82_RS14535"
                     /old_locus_tag="UXR82_14535"
     ARG             complement(6046..7799)
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.38"
                     /coverage="100.0"
     gene            7906..8751
                     /gene="blaZ"
                     /locus_tag="UXR82_RS14540"
                     /old_locus_tag="UXR82_14540"
     ARG             7907..8748
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(9277..9696)
                     /locus_tag="UXR82_RS14545"
                     /old_locus_tag="UXR82_14545"
     CDS             complement(9277..9696)
                     /locus_tag="UXR82_RS14545"
                     /old_locus_tag="UXR82_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_000418035.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINS
                     NLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTL
                     EKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
                     /name="MarR family transcriptional regulator"
     gene            9820..10791
                     /locus_tag="UXR82_RS14550"
                     /old_locus_tag="UXR82_14550"
     CDS             9820..10791
                     /locus_tag="UXR82_RS14550"
                     /old_locus_tag="UXR82_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="WP_000481279.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKDM
                     LALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEYAQ
                     VPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGGVGS
                     VAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLDTVGS
                     NVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIREDIWS
                     KLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
                     /name="oxidoreductase"
     gene            complement(10963..11055)
                     /locus_tag="UXR82_RS14555"
                     /old_locus_tag="UXR82_14555"
     CDS             complement(10963..11055)
                     /locus_tag="UXR82_RS14555"
                     /old_locus_tag="UXR82_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_001791613.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(11212..11559)
                     /locus_tag="UXR82_RS14560"
                     /old_locus_tag="UXR82_14560"
     CDS             complement(11212..11559)
                     /locus_tag="UXR82_RS14560"
                     /old_locus_tag="UXR82_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000119690.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            complement(11578..12195)
                     /gene="cadD"
                     /locus_tag="UXR82_RS14565"
                     /old_locus_tag="UXR82_14565"
     ARG             complement(11582..12195)
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(13311..13505)
                     /locus_tag="UXR82_RS14570"
                     /old_locus_tag="UXR82_14570"
     CDS             complement(13311..13505)
                     /locus_tag="UXR82_RS14570"
                     /old_locus_tag="UXR82_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001004925.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYCL
                     LPLIVNLSIYGFYVFVKRK"
                     /name="hypothetical protein"
     gene            13615..14307
                     /locus_tag="UXR82_RS14575"
                     /old_locus_tag="UXR82_14575"
     CDS             13615..14307
                     /locus_tag="UXR82_RS14575"
                     /old_locus_tag="UXR82_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative immunity/bacteriocin fusion bifunctional
                     protein"
                     /protein_id="WP_000691527.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKSK
                     NIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEANDK
                     NAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKNYVE
                     YKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAVCGTA
                     FAAGCSVG"
                     /name="putative immunity/bacteriocin fusion bifunctional
                     protein"
     ARG             7907..8751
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     Typing          complement(451..598)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.980000000000001e-72"
     Typing          complement(657..832)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.697"
                     /coverage="94.0"
                     /evalue="1.04e-65"
     Typing          complement(964..1946)
                     /name="rep_cluster_2100"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.593"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2373..3115
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttactgtgtg tttgaatttc taatacatga atatcattct ttaaaaacct attaacttgt
       61 ttatctaatt gacgatgagt aaaagtagcg aaagttttaa tcttcatgat atctctcctt
      121 ttataaaaat agcaatcctt attacaatat taaacacttt tattttggat tttaagaggc
      181 attttgaaga cttagaggca aatcatatag gttttgtact taaaaatcta tacagtctct
      241 taaaacgcaa atatgagcca aataaatata tgctaatttc acaaacaaaa atttcagcaa
      301 aatcagtgac taatttttta gatactgccc atttacatgc aaatttaaaa attagcataa
      361 aatactttaa aacttcccca tgtttttcta aattcctgca gggcttttct tatcaaaaac
      421 ttagaaattt taatttaact ctaaaatctc cgtaagagca taagggaaat ttgctatcat
      481 tcaaaaatga tagatctggc aaggccagac attccaaggg ttttaagtta ttttaaagtg
      541 atatcataca aagctgtagt gtcacacttt tgtgacacta catacagcat ttgtcacaaa
      601 aataagacag cactttttta cagaaaaaca ataaaaaaag acatcagtta tcactaatgt
      661 cttaaataat aattttgctt ttcgaaattc caaattgcgg taattgaatg tgcttttttt
      721 cttcgtctgt cgttttatct tcatcacttg tattaatcaa attaccatct tcggcatcgt
      781 ctaagtttaa aattttatgc ttttgtttaa gcaatccacc ataacttaac atacgttttc
      841 gatacaaacc tttttctaaa tcacttacaa tttcttgatt tttttcgtcg tcatcagtta
      901 aaaagtcaga tgatttaacg gaatattttg atgtctcttc gagtgttata aaatcatttg
      961 aattattgat taaagatatt atattgtttc atgtttttac ggcgcatata aagggcatgt
     1021 gtttcttcaa gttcagttag aattgtttgc attaaataag cttgcattga cttgattgtt
     1081 tccttttggc ctttaactcc cctatcatgt atagcttttt taagtctttt taaagaagtt
     1141 aatatttcat cctcaatgtc ctctaacatg taatgcatat cttcatgcct tgaattaaat
     1201 gatttttttg ctttaagaat aacagccttt atgatttcta aatctctata atcatatttt
     1261 aataaataat tagttagttg ttctgggaat tcttgcaata gcatttcttt atcatcaaaa
     1321 ttatgtgtaa attgatttga atgatttgaa tgattactaa tttcattttt gtactttata
     1381 tcattcaagt cattcatatc attattctca gtatcattaa agtcaatgtc attaaagtca
     1441 gtatcattag tgttcgattt tcgaacttct ggacgttcga ttttcgaact tctggacgtt
     1501 cgattttcga acttctggac gttcgatttt cgaacttcct tctcgccgta aggttcaaca
     1561 tcgttttctt ctttttctat tttgtaaata tcacgttctg tgacaatagg tttcattaaa
     1621 tatagaatat ttggtttatt taaaccacgt cgtttttgaa tgagtagacc atcgttttct
     1681 aacccttttt ttatttttgt taacttttct tttttacaat ttaagatttc cattagcttt
     1741 tcatttgaat aaacaaagta aatgttacca tcttcatcaa cccagctatt tttaatagat
     1801 aaatttaagc gatcacgtaa gatagcatag gctattttca tatcattagt tagattttta
     1861 taattttcac tggtaaagaa tactttaggg atttgataaa atctttcttt gtaattttct
     1921 tgcactgtaa aatattgatt ttgcatgaca aaaaacctct ttccatattt tagtttggta
     1981 agaggtaata aaacgttttt atttagcttt acatttattt aatgatttag gtgcttgcac
     2041 taagatatta ttcattatat aatacaagta tcgaaaatta tataaataaa cagtttctaa
     2101 acgttttatt accaaacccc gactattacc agtagttggg gtttttgtta tattaagttt
     2161 attatagcac tttaaataaa cttataaaag ggaaaatata gctttatcaa gggtttataa
     2221 acccttgata aatatcataa acctttcata aacccttgat aaaaaggttg atggatgttt
     2281 atttttattt attaatcact ttattattaa gcattataag cttataatat tttatcaagg
     2341 gtttataaac attttataaa gggttgatga tatgaaaagt gtgaaaaagt tgagtgaaga
     2401 attaggggtt agtaagcaaa cgatttttaa taatattaag cgattgaata tagagacaat
     2461 aaaacaggaa aacacttcgt ttattaaaga agataccgat atagaaaaaa tcattcaaag
     2521 agtaaatgaa aataaaaaga aatatggttt tgaaagtact actgaagaca aacaaaaaaa
     2581 agggtcggat aatattaatt ttgaatcgaa aagcgatgct caaatagtag aaatccttaa
     2641 aaatcagatt gatactttaa ataatcaaat tgaaaaacaa gaaagtcgtc atgaaacaac
     2701 aattgaattt tacagaaaag agttgcaaga aagatcaaaa ttgcttgaaa atcagcaagt
     2761 tctggcatta gagagtaata aaaagattca aaaattagaa aatcaattag aaaaagaaaa
     2821 acaccttaat tatccctttg atacatctac taacactaga caaaacgttg atgcacaaga
     2881 aaaaacatat actactaatc ctgtaaatat taacagagat caggaagaaa ctaaagaaac
     2941 agagattcaa tataaggata tatctggaag tcaaagcgac gaaagtacac aaggtgaaga
     3001 agcaaaaaaa gaagacgttc cagctaatct aaatgacaat gatagtgata tagaagaaaa
     3061 gagtgaagaa acggaaccta aaaaggggtt ttggagtcgt ttgtttggta gctaaaataa
     3121 ttgattaggc actaaaagtt ataaaatttt agtgtctttt atatagtgag aacttctggt
     3181 aatcatcgat tatgtaaact aaagtatttc aaataattaa ctgtattaat actctcatca
     3241 tgattgcgtt ataaacgttc gttattgaga caaaatttaa tagtactact acacaaaaag
     3301 agagtgcgaa ttcgcactct ctttttgtga taaatgaatg gttaagaaaa taattcatcc
     3361 acagttgtat caaacaatct tgccaactta aatgctaatg tcaatgtagg atcatattta
     3421 ttgtttggtt ctgttgcaaa gtaaaaaaat atagctaacc actaatttat catgtcagtg
     3481 ttcgcttaac ttgctagcat gatgctaatt tcgtggcatg gcgaaaatcc gtagatctga
     3541 agagacctgc ggttcttttt atatagagcg taaatacact caataccttt taaagtattc
     3601 tttgctgtat tgatactttg ataccttgtc tttcttactt taatatgacg gtgatcttgc
     3661 tcaatgaggt tattcagata tttcgatgta caatggcagt caggtttaag tttaaaagct
     3721 ttaattactt taaccattgc taccttcgtt gaaggtgcct gatctgtaat taccttttga
     3781 ggtttaccaa attgtttaat gagacgtttg ataaacgcat atgctgaatg attatctcgt
     3841 tgcttacgca accaaatatc taatgtatgt ccctctgcat caatggcacg atataaatag
     3901 ctccattttc cttttatttt gatgtacgtc tcatcaatac gccatttgta ataagctttt
     3961 ttatgctttt tcttccaaat ttgatataaa attggggcat attcttgaac ccaacggtag
     4021 accgttgaat gatgaacgtt tacaccacgt tcccttaata tttcagatat atcacgataa
     4081 ctcaatgcat atcttagata gtagccaaca gctacagtga taacatcctt gttaaattgt
     4141 ttatatctga aatagttcat acagaagact cctttttgtt aaaattatac tataaattca
     4201 actttgcaac agaacctcat ttactatatg ggcatctctt tcattattaa tacaaagctt
     4261 tgtatctccg atattaggca aaggtgtaaa cgaattaggt gatactatag gattcaacat
     4321 tatattaatc gtttctttaa taatgattat cttcttattt tttataaatc gtagtaaaaa
     4381 tatctcctat gattatataa aagttcaaag aggtgaagcc aatgaataaa ttagaaaaat
     4441 ggtctttggt atttattatt tttacatttc tctcttcatt agcactttta tatttcaaat
     4501 ataattttag ttcggttatg ccagtcctaa gtctttctat tttaactata ctaatattgt
     4561 tgacgagtat tattactgta gtttcagtta atgatgaaga tacttaaaac gttcattagt
     4621 gaaacaggta ttatattaaa aaagcgtacg aatttgtacg cttttttctt gtgttattat
     4681 taaaatctca taacaggaaa atattatgag attttagacg ctgtcagaac gtctctttta
     4741 catcgagtga cattgaatca aagtctttgt atgttaatga tatataaaat tatttagtag
     4801 attcattata gtaagctaga ttagttgctc tacttgtttt tattctcttt tgcctctaat
     4861 ttattgagat aacgataaat agttgtacgt gaaacttgcc actgctctgc aattgtttta
     4921 attggcgtac cattatcata aagtgtctta agtaagttta aatcttgttt atttaatttt
     4981 tcaggtctac ctccatatcg gcctctagca cgtgctgcaa ttcgaccggc tgatgatcgc
     5041 tctaaaatta aattacgttc aaactctgca aatgctgcga ataaatgaaa taataattgt
     5101 cctgtagaac ttgatttatc catcgttata ttttcttcta aactatgaaa actcacacct
     5161 cgttcgttaa gttcattaac taacgtgatt aaatcctcca tgttccgtcc tagtctatct
     5221 aatctccaaa caacaatcgt atctcctgac ctcgcaaatt caattgcttt atctaaacca
     5281 ggtcgcttac tttttgaacc actcatatgg tcattaaata tcttttcaca accatatgta
     5341 tttaatcgat cctcctgtaa attcaaattt tgtaatccag ttgaaactct tgcataacct
     5401 attttcaaaa tacagacctc ctatatacac ctttagtgta ccataacttg attacgaggt
     5461 cttttgtgaa catagatttt ttgttgggtt tttgaacaca taattctatg taatacttac
     5521 ttatttagac aaaaaacaag agtgtacaga aaatggttat tttctggaca ctctcatctt
     5581 taataatata ttttaaatgt cattaacaaa actaatttaa taagagtcaa gtatggttta
     5641 catatgtatt atgtttgaaa catacctcta attacttttt actaatatca tttaaaatgt
     5701 ctcgcaattc ttcaatttct ttgttattta attcttcatt tttcgcaaaa ttcagcacta
     5761 aacttttcat atcccctcca tacagtttat taagaaaggt tttagcagtt ttcattttaa
     5821 tatcgtcttc tttaatattt gatgagtaaa aataaatatt ttctgatttg tatcgtttta
     5881 taatctcttt tttatatagt cttgtgatta atgttctaat cgttttatca ctaacttctt
     5941 tatatttttg aatttcaact acaatttcat tagctgatac tgatttttta tcccatatta
     6001 tattcataac atcccattca gccatagata tttcaacttg cttattggtc atttaaaaca
     6061 cccattcctt ttaatatttt ttcacttatt aattcagcat ttttcccaga cgggtttcca
     6121 tctgataaat gtgtagcaaa ataatactta tcatgatttg taattacgta acctacaaac
     6181 cacccattat tatacttccc gtttactata cctgtacctg ttttcccata cagttcatac
     6241 ttttcatttt tcttaatcaa taatgaagaa gataattgat tctttgcttt tttactaaaa
     6301 tggttatttt gttccatcat atttttaaaa actattactt gttcaagatt agatattttc
     6361 aaactatctt ccatccaata gcttttataa cttcccaaat tttcattacc ataatttaat
     6421 tgcttgagtt gagccgcagt atagttcttt ggtatttgat tactaatacg ttcgaagtac
     6481 caattaactg aattttgcat tgctgtattt aaatcttgtt ccttattcca agactcgaaa
     6541 agataatgtt tatgattcca tgacatacgt gaatttttat cacttataat atgacggtca
     6601 agtccaaaca gtgctaaata aattttgtaa gttgaatcag gagaatatct ttttctactt
     6661 tctttttcat tataaatata gtatttgtct ttcttcatgc tgtacataac gaaagatcca
     6721 ctatttgaac caaaattttt actttcatct aaaatttggt aatcgctttg taagggcttt
     6781 ttataattat aatcagttaa ggattgcccc attaaaaact ggctttgaat tatcatgata
     6841 aaaaaagtaa aaatacaaat aaaaattaga attaattttg actgcttttt cagatcggct
     6901 tcttttatat taattaatct acttttgagt aatgattttt taccattaaa tgaatggctt
     6961 aatatatttt tgttggaaaa tggggttttt aatacggagt ccataataga ttccgcataa
     7021 gttttaaatt catttttgtt aatattatta aggacaaatc tatcggcttc tacttcattg
     7081 tcatgaatta tctttctttt tacaatatgt actaatggat tgtaactcat aataatagag
     7141 aagatattaa agataattaa atgtaaagta tctctatttt tagcatgagc atattcatgc
     7201 agtattatat attttagtct tttatcaatt acacttttaa aatatgaact aggaatcaaa
     7261 ataatatatt tcccatacca aaaagttatt ggagattgaa tagcctctgc ttttcgaatc
     7321 acaatatttt ttttatattg atggttgaaa agtatcgtat ctattttatt tttttcattt
     7381 tcgtttagat aaagtgactg tttctttaaa tatttaagat ataataaggc tttcaaaaat
     7441 ttaaaactta aaataataac taaaactatc caaataactg tgcagatatt atcaattgaa
     7501 tcccaattaa acttatggat atctgttgtg aactcttgaa taggtttggt ggtatttatg
     7561 ttatggttca aatcgtgtga cttactttca actgtgggcg cttgattatt cacattatta
     7621 aattttataa aagagaattt aattgggata aaaggaatta atccagcaag catagttaga
     7681 taccaaactt tataatttaa actataatta aaatagcgtt ttaatatgta cctaaaaaat
     7741 actaacaata gaaatataaa acaaaagctt actacgctca ttattagtaa tttagccatt
     7801 ttgacacctt ctttcaaata tttataataa acaattgaca tcaatattac aattgtaata
     7861 ttattgattt ataaaaatta caactgtaat atcggagggt ttattttgaa aaagttaata
     7921 tttttaattg taattgcttt agttttaagt gcatgtaatt caaacagttc acatgccaaa
     7981 gagttaaatg atttagaaaa aaaatataat gctcatattg gtgtttatgc tttaaatact
     8041 aaaagtggta aggaagtaaa atttaattca gataagagat ttgcctatgc ttcgacttca
     8101 aaagcgataa atagtgctat tttgttagaa caagtacctt ataataagtt aaataaaaaa
     8161 gtacatatta acaaagatga tatagttgct tattctccta ttttagaaaa atatgtagga
     8221 aaagatatca ctttaaaaga acttattgag gcttcaatga catatagtga taatacagca
     8281 aacaataaaa ttataaaaga aatcggtgga atcaaaaaag ttaaacaacg tctaaaagaa
     8341 ctaggagata aagtaacaaa tccagttaga tatgagatag aattaaatta ctattcacca
     8401 aagagcaaaa aagatacttc aacgcctgct gctttcggca agactttaaa taaacttatc
     8461 gcaaatggaa aattaagcaa aaaaaataaa aatttcttac ttgatttaat gtttaataat
     8521 aaaaacggag acactttaat taaagatggt gttccaaaag actataaggt tgctgataaa
     8581 agtggtcaag caataacata tgcttctaga aatgatgttg cttttgttta tcctaagggc
     8641 caatctgaac ctattgtttt agtcattttt acgaataaag acaataaaag tgataagcca
     8701 aatgataagt tgataagtga aaccgccaag agtgtaatga aggaatttta atattctaaa
     8761 tgcataataa atactgataa catcttatag tttgtattat attttgtatt atcgttgaca
     8821 tgtataattt tgatatcaaa aactgatttt ccctttatta ttttcgagat ttattttctt
     8881 aattctcttt aacaaactag aaatattgta tatacaaaaa atcataaata atagatgaat
     8941 agtttaatta taggtgttca tcaatcgaaa aagcaacgta tcttatttaa agtgcgttgc
     9001 ttttttctca tttataaggt taaataattc tcatatatca agcaaagtga caatagcatt
     9061 ttacaagtat tttttattgt tcgattaggg taaacctaaa taatacataa attatattac
     9121 aatatgttta ttatagaggc taaagtatct tagaaatttg gatagtaggt ttttcgggaa
     9181 taaaagtata gttggcttta atatataatt aagctaagct atcatttata tttactcgat
     9241 tataattcgc acttcaactt ttttataacg taaaaattaa aacgtagttt tatttatctt
     9301 tagtaatatc tttttaaaca ctacagtttc ttcattagtt aaattagcga agtaatgatt
     9361 attaagttga taggctttct tttctaatgt tggccataag tcttttgctt tatttgtact
     9421 gtttattagc acagttcttc tattttcatt atcagtttga cgtgttatta gatccttttt
     9481 ggacatctta tctaatattc cagatattgt tgaaggttct aaatttaaag tattaccaat
     9541 cgctttaggt gtattcaaat tactattaat caagcaattc aaaacaccat attctgctgg
     9601 agtaatatta tagggactta ataaactttt aaaatgttta aaaactctat tctgagaagt
     9661 actcaacaaa taatttatac aattatctaa ttccacaact aaaacctctc ttaaatcatt
     9721 agtattgact cgcttatcat atcatgataa gttttgtagg aaaatagttt tgcaacaaaa
     9781 acatacttct acaaaacaat tgtgaaagaa ggctaataaa tgttcaatgc ggtagtagtg
     9841 agaaaaagtg catcaaatgt tagttataaa gtagaaaaag ttgatgaatc tcatttatcg
     9901 aagggtgatg tattagtaaa agtggtatac tcttcaataa attataagga tatgctagct
     9961 ttacaatata aaggcggtgt aattagagat tatcctatga ttcctggtat agattttgca
    10021 ggtattgttg aatcaagttc gaatgataag tttaaagaag gcgataatgt attagttact
    10081 ggttatgatg ttggcgtgac acatacaggt ggattttcag aatatgcaca agtgcctgga
    10141 gagtggattg ttcctattcc ccaagaaatg tctattaaag agtctatggt atatggcaca
    10201 gccggtttta cggcagcatt atcaattttt gaactagaaa aatatggaat gaatatcaaa
    10261 tcacaacctg aaattttagt gtctggggct acaggtggtg tgggcagtgt tgctatacaa
    10321 attttacata aaattggata tgaaaatatc actgctatcg taagaaaaga aaagcaaata
    10381 gaaatcgcaa aaaaattagg tgcaaaacaa gtaataataa ttgaagatga taataaaccg
    10441 cttaaaaaag gtatttttga ttatttctta gatacagtgg ggagtaatgt aacattatac
    10501 ggtttaaaac gtctaaatta tcaaggtgtt gctactgtat gtggaaatgt agcaggaaat
    10561 tcacttaacg ctaatatatt accgtttatt cttagaggta ttaaattaat tggcattgat
    10621 tctgtatatg ttgatcataa tatccgtgaa gatatatgga gtaagttagc aaatgaatgg
    10681 aatataggaa attcacttct atacaatgaa attggatttg aagaattcta taaaacgata
    10741 gatcagcttt taaaaggaca acatttagga agaactattt taaaagtata agtgtgagta
    10801 ttcattaata catttactat taattcaatc attttgcatt tctctactaa ccagtgttat
    10861 aatttatata acgaaaatga ttaacaatta atgcaccaat ccccacacta ttgcagtagt
    10921 gtggggattt tttcgatgct ggcttataaa gtcgcctatt aattatttgt tgcgtttact
    10981 aagccaataa gtataatacg caacagcaca gccactgatg actggtgcta tgatgtgaac
    11041 gaaaatgatt agcaaagtaa tgcacctcct ctcttcgtca attgacgcct gagagatagg
    11101 cgactctatt attataccat ctgtgaatat acaagcatat ctacttccga taagtcgtct
    11161 tatgttatat aactttgttt tttatagtat caagacaaga agaaactcgt ttcaaaaacc
    11221 ctcatgattt ttagagaaaa actctcttat ttcatcgtct ttaataaaat aatatgccat
    11281 ctttccatct ttataaaaat caagtacctc atttttatat aaaagcctta aatgatgtga
    11341 agttgaagca acactcattt tcaatatcaa agaaatatca caaacacaca actcatcttc
    11401 tttaatcaaa gataatatga ttttcaattt cttctcatca caaatttttt ctaaaatgtt
    11461 aagtaatttc ttagatttat catcttctaa aaaacttaaa gcattgttaa ctttatcctc
    11521 atgtacacag atcacatcac aagtattttc ataactcata taaaatcaca ccctaaatta
    11581 aaatataaat cctaaaattg tttgaatagt gtcattttca ataataataa ataaacctaa
    11641 agctatataa ataatagcca taatccaacg actaaatttc tcaacaattt ctccaattcc
    11701 tggaatatta gctaattttt gtgcagtaaa caccaagaag aaaattaaaa ttaaaaagac
    11761 aaacagagta agtaataaat tagtaacact taatgtcaca aaatacggaa caaataaacc
    11821 aatattatcg gcgccacaac ttgctatcgt aacaattgca accgtaccaa ctaatttaga
    11881 caatcctttt tcattcaatt cttttttagc tctcttttct ccttcacaat catcataaat
    11941 agccacttta attccaagat aaattggtat taaacccaat aaacctaata tccacttttc
    12001 tggaacataa tttaatacaa aagctaaaaa caaactaact aatattaaaa taatagaccc
    12061 taaatattga ccaacataaa tatctctata ttcttttcta gtctttgctc tagcaaaaaa
    12121 tattaataaa attactaata aatcaactgc tgtagcaata taaagaacag cagcagcaac
    12181 aaccgtttga atcataatac acctcattca aaaatatttt tgaatgtatt ataacttata
    12241 tttttaaata atcataggtg ctttattcca attgctttat tgacgttgag cctcggaacc
    12301 cttaacaatc ccaaaacttg tcgaatggtc ggcttaatag ctcacgctat gccgacattc
    12361 gtctgcaagt ttagttaagg gttcttctca acatcaataa attttttcgg cataaatgcg
    12421 tgtctatgcg tagacatgga gacacttcag aattatggag tgattacatg agtttaacta
    12481 tttttcttta ttcagtttta ggaagtttat tagcaaatta tatctacgaa aaatcttcaa
    12541 aataaataac tttttcggtt ctactttgta cactttatct acctggtaga cctaattgtt
    12601 attgctttac aggtctaaaa ttcgtcgtta taatctagtt aaatcccgta agggcgcact
    12661 tatacgtcgg ataaatcgac atgtgcaaaa acgtaatcga ccttcgtcga tttttttatt
    12721 tcaaaattaa agaaacggct tgagatattc cgagcagtta taaaaattaa tttgcgttga
    12781 atattgctac tacattcttg aaaaagtgat gtagatttat agcccataac ttttttgaat
    12841 tacatcgacg atacggcgtc gaaagaaaaa taaatagacc gtatgaaaga aaaagctacc
    12901 tcactttatc tggggtagct tttatttttt atgcccttat tttgaatttt aagagggcat
    12961 tttaaagatt taggggtgat tcatatagtt ttatatctaa aacttatata tgcttttaaa
    13021 acgcaaatat gagccaaata aatatatgct attttcaaaa acaaaaattt gagcaaaaac
    13081 aataactatt tttagacact gcccatttac atgcaaatta aaaattagca taaaattctt
    13141 taaaacttcc ccacgttttt ctaaattcct gtagggcgtt tttatcaaaa atcaagacaa
    13201 gaagaaactc gttcacactc gtttcaaaaa aacaagaaat ctaatctata cacctaccgt
    13261 tttccaaccc cctcaaagca tacaaattaa aaacaaaatt attcataatc ttactttctt
    13321 ttaacaaaaa cataaaaacc ataaatagac aaattaacta tcaaaggcaa taaacagtat
    13381 aaaaaaacca cgaataaatc ttcatcaaca tctaaaataa aaaagaataa aacaattaac
    13441 aaaatactaa ttaacaaacc taaaagtggc aaacttttta tatatttttc taaattttca
    13501 ttcataaaat agactccttt caaaaatatg tttataaata tattaaaata aatattgatt
    13561 ttataaagcg cttacttata caattaaaat atcttattat cttggaggta tcaaatgaaa
    13621 attataaaat tattttcaat aatttccttg ttcctactgg tatttagcgc gaatttttct
    13681 aatgcttacg caaatgaaga acaaaaaagt tctttactag aaaatcaaaa agaaaaattg
    13741 aaaagcaaaa acatcgaagt atcacaaatg accaagaaag aaataagttt tctagaacaa
    13801 ttgcaaaata aaaatgaaga tatgcaaaaa ttcgaaaagc aaatgaaaaa agaaggattt
    13861 aaaaaaataa agctagaaga caaaaattct aaattaaaat atctagctac agaagcaaat
    13921 gataaaaatg catatggcta catagctaat aacgcttata aaaatgaaaa aacaaaagaa
    13981 attgtagttt ctgaaacaat atatgataac tacaacaaaa aaatcacaaa attcattgcc
    14041 gaaaaaagaa gtatgactga aaacaaagag ggaaaaatac ttattaacaa aaattacgtt
    14101 gaatataagg ctgaaaaagt tgataaaaac agaccggtaa catatgcagg attcaaatgg
    14161 aacggcaaag cattcgcttg tagtatggct ggactttatg catgtgctca ctactgtgga
    14221 gtatgggcaa tcgtaaatcc aatagccggc ggtgtatgtg aagcagtttg cggaactgca
    14281 tttgcagctg gatgctctgt aggttgattt tattccaatt gttttattga cgttgagcct
    14341 cggaaccctt aacaatccca aaacttgtcg aatggtcggc ttaatagctc acgctatgcc
    14401 gacattcgtc tgcaagttta gttaagggtt cttctcaaca tcaataaatt ttttcggcat
    14461 aaatacgatg ttccttattt atcctcataa aaaacaatag ctgaaatata taaccaa
//
