LOCUS       NZ_CP147182             5596 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain SC7 plasmid p3, complete sequence.
ACCESSION   NZ_CP147182
VERSION     NZ_CP147182.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40144595
            Assembly: GCF_037157685.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Wang,D.
  TITLE     Klebsiella pneumoniae strains whole genome sequences
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Wang,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2024) School of Life Sciences, Taizhou University,
            1139 Shifudadao Avenue, Taizhou, Zhejiang 318000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.15.3; Canu v. 2.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina NovaSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037157685.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 18:19:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,489
            CDSs (total)                      :: 5,366
            Genes (coding)                    :: 5,239
            CDSs (with protein)               :: 5,239
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 45 of 127
            Pseudo Genes (incomplete)         :: 82 of 127
            Pseudo Genes (internal stop)      :: 16 of 127
            Pseudo Genes (multiple problems)  :: 16 of 127
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147182.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SC7"
                     /isolation_source="bronchoalveolar lavage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p3"
                     /geo_loc_name="China: Taizhou"
                     /lat_lon="28.40 N 121.26 E"
                     /collection_date="2021-02-27"
                     /collected_by="Dongguo Wang"
     gene            complement(join(5444..5596,1..60))
                     /locus_tag="WBS29_RS27395"
     CDS             complement(join(5444..5596,1..60))
                     /locus_tag="WBS29_RS27395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(67..576)
                     /locus_tag="WBS29_RS27400"
     CDS             complement(67..576)
                     /locus_tag="WBS29_RS27400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<611..1315)
                     /locus_tag="WBS29_RS27405"
                     /pseudo=""
     CDS             complement(<611..1315)
                     /locus_tag="WBS29_RS27405"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1254..1598)
                     /locus_tag="WBS29_RS27410"
     CDS             complement(1254..1598)
                     /locus_tag="WBS29_RS27410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(1588..1764)
                     /locus_tag="WBS29_RS27415"
     CDS             complement(1588..1764)
                     /locus_tag="WBS29_RS27415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            2404..2562
                     /locus_tag="WBS29_RS27420"
     CDS             2404..2562
                     /locus_tag="WBS29_RS27420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            2920..3246
                     /locus_tag="WBS29_RS27425"
     CDS             2920..3246
                     /locus_tag="WBS29_RS27425"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            3272..3817
                     /locus_tag="WBS29_RS27430"
     CDS             3272..3817
                     /locus_tag="WBS29_RS27430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            3883..4233
                     /locus_tag="WBS29_RS27435"
     CDS             3883..4233
                     /locus_tag="WBS29_RS27435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(4303..4569)
                     /locus_tag="WBS29_RS27440"
     CDS             complement(4303..4569)
                     /locus_tag="WBS29_RS27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4553..4777)
                     /locus_tag="WBS29_RS27445"
     CDS             complement(4553..4777)
                     /locus_tag="WBS29_RS27445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     Typing          complement(1851..1927)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          2116..2513
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(3..1264)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.762"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cgagtagtcc tgctgtagct gctctaatgc gctcagcaac tgtttttcca gctcggtcat
       61 cggcacttac tccttcacca gtctcagcgc gttctgcttc gaccgcccgt tcccgaccgt
      121 ccagctctgc gtcctgtcta cggccgtccc cttcggcagc accagaaaac gtccgttgct
      181 gtcttccagg tacgtcacgc cccaggtctt cgcgttcagc ttcgacagcg ctgcgttctg
      241 ccggtcgatt tcggccagat ttgacgctat caagctcccc tgataccaca acaccccgct
      301 caggctggcg aacagcagtc ctgacaccat cgtgatggtc agccatgtcc gcccgaccat
      361 acgcatcacg ctcaggcggt tcgactgcat ggccgcgctc ataccctgct cgtgggcgcg
      421 gatggcggcg ctgattctct tcccgctttc gctcagttcc gctctgacag acttctcgtg
      481 ctcgctgaat gcggctttca gcatttcgcc ggtactctgc tgctgcgctt tcgatttctg
      541 ctctaagtcc ttcgccagcg ttaaaagact gttcatagat ggctccctta agtcggatgt
      601 ttcgccctcc gtccgggtcg gcaatgctga tgctgctctt tgtggtgcgc acgacctcaa
      661 aacctgcgct ttccagcgcc tcagtgacgt cctgacgcgt tttaagctcc cctgacgagg
      721 cgagggccag taagccccgc gtaatagcct ctgcggcttc ctgcttcgtt ttgggtaacg
      781 aagatggcgt caccagcgcg cgccggttct ccggtgcgtt cgggtcgtgc agccccagtc
      841 gcccgttcac cacggtctgc cacgcatcga tgcgcggacg gtctgcccgg tcgtagtacg
      901 gctggagacg cctgccggtc agcagttccg tgttcggtac cagaaaattc agttccagcc
      961 gccccttgtc ccggtgttca acccacagca cgctgtactg gtctttatcg agtccgggca
     1021 tcagaacccg ctcgaagctc gccatcagct tttcacgctg gccgggcggt aaatcctgtt
     1081 cagcaaaaga caggacgccg gaggtgtact ttttggcgta gggcgaggcg tcgataagct
     1141 cccggacctc ctccggcttt ccctgcagga cgatggcccc gtcgcgctgg cggtctttcc
     1201 ccagcagata atccaccggc ccggcaccgc cgcctcgccc tcgcggatga aacttaacga
     1261 tcatcgtctg cccccttttc cagcacggca tgccgcagcc gctcgagttc ggcatcaatg
     1321 gccatcagcg cggccaccac ctgaacccgg tcagcgcctg accactggcc gccgttaatt
     1381 ttccgggcga tctggttgag gttgttcccc atcccggcaa gctggcgcag caaaaccgga
     1441 tcgatggacg gcagccgcaa cgaccgcgcc gggcgcgtat cgagacaggt ttcccgcatc
     1501 cacaccgcca gttgccttcc attgcagcgc tccagcagct gctggtactc cccatcggtc
     1561 acccggatac ctaaaaaacg ggtacgctta accgccatcc tctcctccga tatccgcaaa
     1621 atgtgaccgg gctgaacgct caagctctgc catctgctcc cggcggtaat cttcctcctg
     1681 tgcctgacgc atcagatttg ccatcactgc cggatgccag gatctttttt ttacgacgag
     1741 cggcttcttc ttcggcttct gcattatccc tccttcgcaa acgccggacg gcacggaaaa
     1801 gttttccggg tcgctccggc gttggacaag cgccagcgcg tcagtggtgt tagcgggttt
     1861 cggggcgcag ccctgaacca gtcacagcgt ttacgctgtg tatactggct tactatgttg
     1921 gcatgagagc acgattcatg aaagtgcctc atgtggcagg tgtgatatgt ggctcaggag
     1981 cgtccgcaat accatgcacg gacagatgaa atgacgcctc ctcgctcact cggtagctgc
     2041 gctcctgccg ttcggctgcg gcgggcgtga ccgactcacg gataacggga taaagcaaag
     2101 gcgctcaaat cagaaagggc cggagaaaag gatgcggcgt tgccgttttt ccataggctc
     2161 cgcctcccct gacgagcatc acagaaatgg acgctcaagt cagaggagga cgaaacccga
     2221 caggacttaa agatgccggt gatttctctc cggcggctcc ctcgtgcgct ctcctgttcc
     2281 gaccctgccg tttaccggat acctgttccg cctttttccc tttcgggaag cgtggcgctt
     2341 tctcatagct cacgctgctg gtatctcggc tcggtttagg tcgttcgctc caagctgggc
     2401 tgtatgcacg aaccccccgt tcagcccgac tgctgcgccc tttccggtaa ctatcatctt
     2461 gagtccaacc cggacggaca cgcaaaaacg ccactggcag cagccattgg taactggata
     2521 tatacgagag agatattgag aggtcttgaa gtgggtgcct aactgcggct acactgaaag
     2581 aacagttttg gtatctgcgg tctcgtatac ccagttaccc ggttaagcag ttccccaact
     2641 gacttaacct tcgatcaaac cgcctcccca ggcggttttt tcgtttacag tgcaagagat
     2701 tacgcgcaga aaaaaaggat ctcaagaaga tcctttaaat gatcgtcagt tctgttatcc
     2761 ccggagctcc atccatccca cggaaaaaca cccgccactg atgccctcag cgcactgcac
     2821 atcatgtaca aaccactccc tccctccttt tttattgcgt ttcttaatgc gcaatggtag
     2881 ctttgtgcgc aacaaaaaac gcaatagcaa gggggggtaa tgagagaaag aacaaatatc
     2941 atgctagata taggattaaa gcaatcctta caacggcttt cgaataaaac caaaatgagt
     3001 ctaagtgatc ttattaacca gagattatca ggttcacttt cagttgaaga acgcattggc
     3061 gaaaagcctg aatggatgag aaaccttgat ccctatctcg ccaatcactt ttcaaatatt
     3121 gagttaccgc tcccttcacc attaaaagaa taccaagaaa aaatggatga gatagattca
     3181 attattaatg acatgatggt tctaaaggaa tatttcagag ctaagtttta cgacgaaata
     3241 aaataggcac gctatacgga ggcggagaat tatgtttttt aaacggtatt taacggagaa
     3301 ggaaatatac aaggcagcac tcccaattgt taggcttcaa gggagagtta acacttcatt
     3361 acgtttttta tccaaatact cagatgtaaa caaaaatgga ttaattgcat tgcatcttaa
     3421 tttattacat tttatctcat acaacataca cggtgtagag tctctgaaaa tcactcaagc
     3481 aggcataaac aaagctgtac aacaaagccc tcacgcagat gaaataaaaa atattgcgca
     3541 ggcaatagga cgcaaaggac ctgagtacat cgaaggatta attagaaacc catgggggaa
     3601 gaaaaatgat aatccactaa tatttataat gagcattgaa gaaagtataa aagaaaccct
     3661 taactcaggt tatatgttat atagagtagc tcccataact gagggtgaag cagctgcttt
     3721 catggatgtt cttaaacaat tattattatt tgaggataat tttcgccttt ttggtagaga
     3781 cttggtttat aaaatagaaa aattaaaaat cagataacaa agtatggtga acgaaaaatg
     3841 aaagagagga tagtgaaatg tgtaatacca agattctcaa ctatgtattg gttaattgcg
     3901 ttcctagcat ttatattccc cggtcgatct tttgctgacg gttacaccgc ttactaccga
     3961 tgtaatggta ctcaggaagc atctcttact gttaaagata acaaactaag tatggttgta
     4021 ggtctttcta agtttaatag gcaactggat acttatagat tcaaagaaaa gggaatcgaa
     4081 gtagtaatgc agagagctgc ttctgatgat aactctgagg ccataatgtc ttttattatg
     4141 cctgacgtta acaatatgac cttattagtt aaaaataacg gctacattat cgttaagaca
     4201 acctgctggt taactaaaac cgacaaacag tagaacacgt agttagtcga gtatttacca
     4261 taatacctgt tgtcggcatc aaaaaagccc gcatagcggg cttcagtcgt aaacttcgcg
     4321 gcgatgtcca atcgtggcgg caaggataac aagctcgtca tctcggatct cacacagaac
     4381 gcgataatct cccacccgat agcgccagaa ctcgcccagc tcacctttga gcggcttccc
     4441 tgactggcga ggatcggcag caactgcaat gcgtaaactc ataaaatcca caatccgtcg
     4501 tgcgttctgt ttgtccatct tgcgtaacga tttgagcgcc cgatcggaat agttaatcgt
     4561 ccagaccaag tgacttcctc acgtcttcag acgaatgcac tgcttcatca ccacggcgaa
     4621 cgcgtgcagc agtttctgct gaaagataat aatcctccag gtcttccaga tgcgccagta
     4681 ttgcttcccg cgcataaaac gtctttgttc tgccggtttg cttcgccagc gagtccagac
     4741 gggactcaat ctcatcagat aaccggatag caagcatata aacctcccga acaccatttg
     4801 ctatacatgt atatcaacag taccacaatc aacctaaaag aacaaaaaat aatcttgtta
     4861 gcccctcttg ataatccgta acgcggtcaa tatatctagc ttgagggctt tcctcttaac
     4921 tattgaaagg ataacgatta tgggtcttaa gccgggtccg aaacgaattg ccgattcaac
     4981 aggggaacct gataaacgcc agcgtgacaa taaaaaaacg ccgggaaata ctgacaaatt
     5041 gaaacccagc aaatcttcaa aaaaataagt cagtagttaa aagccctccg gggctttttt
     5101 gtatctgccg ttcacgctga ttaaacataa cacccgttgc ccgcaccgcc ccggacgggt
     5161 tcgccgcgaa cggggtggca gtcaggtaaa accggcaaca aaccacccga taaaccgccc
     5221 cgttcgcgac agataaattt tacctggctg catagccatg cagccaggtg aatgacatcc
     5281 ggtttcagcg taaatgcccg atgagaaccc tgttttcctg catttcagcc cagcgacgga
     5341 ccgtcatagc ttcgctgccg ggaacgcgct tccgtcagtt ccttttcgtg ctgccgttgc
     5401 tcgtgcgccc gttctgcggc cacggccatc tcatgacgct gcactaccgg ctcaaacgct
     5461 gcagttgccc gctcaagcgt tccacctgct gactcaagcg cgtcacgcgc tcgctcagcg
     5521 ccgcgttctc ccgttgcata agccctgaca ttttccgcca ttccccgaag gcgctctccc
     5581 actcgtccag cctttt
//
