LOCUS       NZ_CP147181            10059 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain SC7 plasmid p2, complete sequence.
ACCESSION   NZ_CP147181
VERSION     NZ_CP147181.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40144595
            Assembly: GCF_037157685.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10059)
  AUTHORS   Wang,D.
  TITLE     Klebsiella pneumoniae strains whole genome sequences
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10059)
  AUTHORS   Wang,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2024) School of Life Sciences, Taizhou University,
            1139 Shifudadao Avenue, Taizhou, Zhejiang 318000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.15.3; Canu v. 2.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina NovaSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037157685.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 18:19:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,489
            CDSs (total)                      :: 5,366
            Genes (coding)                    :: 5,239
            CDSs (with protein)               :: 5,239
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 45 of 127
            Pseudo Genes (incomplete)         :: 82 of 127
            Pseudo Genes (internal stop)      :: 16 of 127
            Pseudo Genes (multiple problems)  :: 16 of 127
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147181.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10059
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SC7"
                     /isolation_source="bronchoalveolar lavage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2"
                     /geo_loc_name="China: Taizhou"
                     /lat_lon="28.40 N 121.26 E"
                     /collection_date="2021-02-27"
                     /collected_by="Dongguo Wang"
     gene            join(10052..10059,1..1678)
                     /locus_tag="WBS29_RS27330"
     CDS             join(10052..10059,1..1678)
                     /locus_tag="WBS29_RS27330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            1688..1945
                     /locus_tag="WBS29_RS27335"
     CDS             1688..1945
                     /locus_tag="WBS29_RS27335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            2030..2179
                     /locus_tag="WBS29_RS27340"
     CDS             2030..2179
                     /locus_tag="WBS29_RS27340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            3662..5626
                     /locus_tag="WBS29_RS27345"
     CDS             3662..5626
                     /locus_tag="WBS29_RS27345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            5626..6357
                     /gene="mobC"
                     /locus_tag="WBS29_RS27350"
     CDS             5626..6357
                     /gene="mobC"
                     /locus_tag="WBS29_RS27350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            6364..6894
                     /locus_tag="WBS29_RS27355"
     CDS             6364..6894
                     /locus_tag="WBS29_RS27355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6921..7100)
                     /locus_tag="WBS29_RS27360"
     CDS             complement(6921..7100)
                     /locus_tag="WBS29_RS27360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7126..7554)
                     /locus_tag="WBS29_RS27365"
     CDS             complement(7126..7554)
                     /locus_tag="WBS29_RS27365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(7718..8032)
                     /locus_tag="WBS29_RS27370"
     CDS             complement(7718..8032)
                     /locus_tag="WBS29_RS27370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8020..8298)
                     /locus_tag="WBS29_RS27375"
     CDS             complement(8020..8298)
                     /locus_tag="WBS29_RS27375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(8473..8838)
                     /locus_tag="WBS29_RS27380"
     CDS             complement(8473..8838)
                     /locus_tag="WBS29_RS27380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(8835..9206)
                     /gene="tolA"
                     /locus_tag="WBS29_RS27385"
     CDS             complement(8835..9206)
                     /gene="tolA"
                     /locus_tag="WBS29_RS27385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(9480..9725)
                     /locus_tag="WBS29_RS27390"
     CDS             complement(9480..9725)
                     /locus_tag="WBS29_RS27390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             1..6678
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2283..2860)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.942"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5627..6354
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 tggagacggt cgaggtccgg gtaattcagg tctgggacat aatggtggtc aggccagtgg
       61 gaatgtgaac ggtacgtctg gtaaaggtgg cccttcatca ggtggtggta cggatccaaa
      121 cagcgggccg ggctggggta cgacgcatac gccgaatgga gatattcata actataatcc
      181 gggggagttt ggtaacggcg ggagtaaacc cggtggtaat ggtggtaaca gcggcaatca
      241 tagcggtagc tctggtggcg gacagtcttc ggccactgcg atggccttcg gtctgcctgc
      301 cctggctact ccgggggctg aaggactggc tttatcagtt tccggcgatg cgttgtcggc
      361 cgccgttgct gatgtgctgg ctgccctgaa ggggccgttt aagtttggtc tgtgggggat
      421 tgcgatctac ggtgtgctgc cttctgagat tgcaaaagat gatccgaaaa tgatgtcaaa
      481 aattatgacg tcattaccgg ccgatgcggt gacggagact ccggcaagta ctttaccact
      541 ggaccaggcg acggttcgtg tcagacaacg ggttgtggat gtggtgaagg atgagcggca
      601 gcatattgcg gttgtcgcag gtcggccaat gagtgtccct gtggtggatg cgaaaccgac
      661 aaaacgtccg ggggtattca gtgtgtcgat tccgggtctc ccgtctctgc aggtgagcgt
      721 accgaaaggt gttccgacag cgaaagcccc gccaaaaggc attgttgctg aaaaaggtga
      781 ttcacgtccg gctggtttta cagccggtgg taactcccgt gaggccgtta ttcgtttccc
      841 gaaagagacc ggacagaagc cggtttatgt gtcggtgaca gatgttctta ccccggcaca
      901 ggtaaaacag cgtcaggagg aagaaaagcg tcgccagcag gcatgggacg ccgctcatcc
      961 ggaagagggg ctgaaaagag actatgataa agcgaaagct gagctggatg ccgaagataa
     1021 aaatattgcg accttaaaca gccgcattgc atcgacagag aaggcgctcc ccggtgcaag
     1081 ggctgctgtt caggaagccg ataaaaaggt aaaagaggca gaggcaaata aagatgattt
     1141 tgtgacttat aacccgcctc atgaatatgg ctccgggtgg caggatcagg ttcgctatct
     1201 tgataaggat attcagaatc agaatgagaa attaaaagcg gctcaggcat ctttaaacgc
     1261 aatgaatgaa tccttatcca gagataaggc tgcacttacc ggggcgatgg agagccggaa
     1321 acaaaaggag aaaaaagcga aggatgcaga aaataagtta aatgaggaaa agaacaagcc
     1381 tcgcaagggg actaaagact acggccatga ttatcatcca gtacctaaaa cagaagacat
     1441 aaagggactt ggagagttaa agcgaggtga tcctaaaaca cctaaacaag gtggtggtgg
     1501 taagcgggcc cgatggtatg gcgataaaaa acgtaaaatt tatgaatggg attcccagca
     1561 cggtgagctt gaagggtatc gtgccagtga tggcgaacac ctaggcgcat ttgatcccaa
     1621 aacgggtaag caggttaaag ggccagatcc aaaacggaac attaaaaaat atctttgaga
     1681 ggtaaatatg gggcttaaat tacgtttgac atggtttgat aagaaaactg aagagtttaa
     1741 aggggaagag tattctaaag actttggtga cgatggttct gtcattgaaa gcttgggaat
     1801 gcctttaaag gataatatta ataatggtga gtttgatttg aagaaagaat gggttccttt
     1861 actacagcca ttttttaaaa atcaaataaa acctgatgtg gataattgtt atatagcttt
     1921 tgattatcgg gacggtaact ggtaacaaaa aattatcaag gatgatttga aatactgact
     1981 tccggtgttc agggatgaat gccggagtct ttaaatacag ggaaagtata tgaaaaaggt
     2041 aaagactatt tttcttttta tattgattgt tgctggcttt cttctggccg catgtcaggc
     2101 aaattatatc cgggatgttc agggcggaac ggtggcacca tcgtcctcct ctgaactgac
     2161 ggggatcgcg gttcagtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2221 gtaatctttt gccctgtaaa cgaaaaaacc acctggggag gtggtttgat cgaaggttaa
     2281 gtcagttggg gaactgctta accgtggtaa ctggctttcg cagagcacag caaccaaatc
     2341 tgtccttcca gtgtagccgg actttggcgc acacttcaag agcaaccgcg tgtttagcta
     2401 aacaaatcct ctgcgaactc ccagttacca atggctgctg ccagtggcgt tttgcgtgct
     2461 ttccgggttg gactcaagat gacagttacc ggataaggcg cagcagtcgg gctgaacggg
     2521 gagttcttgc ttacagccca gcttggagcg aacgacctac accgagccga gataccagtg
     2581 tgtgagctat gagaaagcgc cacacttccc gcaagggaga acggcggaca ggtatccggt
     2641 aaacggcagg gtcggaacag gagagcgcaa gagggagcga cctaccggaa acggtgggga
     2701 tctttaagtc ctgtcgggtt tcgcccgtac tgtcagattc atggttgagc ctcacggctc
     2761 ccacagatgc accgaaaaag cgtctgttta tgtgaactct ggcaggaggg cggagcctat
     2821 ggaaaaacgc caccggcgcg gccctgccgt tttgcctcac atgttagtcc cctgcttatc
     2881 cacggaatct gtgggtaact ttgtatgtgt ccgcagcgcc cgccgcagtc tcacgcccgg
     2941 agcgtagcga ccgagtgagc gaggaggcgc aatttccccg gcacctgcaa gacggtcacc
     3001 ggacgcgctg atgtgggata ttgtcccctg attaagtctt tctattccct gacgatcaat
     3061 tatcaggata ggtgaaccat gtcggttttt cgctgaacat tttacgttaa cctatctctg
     3121 ttgctttttt ctccttgcgg agaaatgcaa gtcatccccg ctggggcgtg gctgacttca
     3181 ggggaaaaga ccttgcttac gctggctgga cgccaccgac gcacggtctt ttcccctctt
     3241 tctggctctg tccggctgga cgccggccac gccagaaatt cacccggccc ccgtcaggct
     3301 cacgcctgac cacccacaaa aatccacggt gctggacgca cctgacgaag cagcaaagct
     3361 gctcctgatt ttgcgggtgg tcagccgctc gctatggcga actctgacgg gggccggaac
     3421 accggaaaag accgcactgg ggtgccgcct tttcctctca cacgctgggg cgtgctcgtt
     3481 gttcctccgg ggctgcgcct tttccgcatg gggcggaaag aggctggcct ccgcggtgaa
     3541 agctgaacgc aatcaccacg gacgtgaccg ccgccgccat gccttggggc atgtccgcgg
     3601 cttgtgcagc gggggctgcg catcacgccg gtaaacccgt cgtctgaagg agagagattg
     3661 catgtttaat acggattcgc ttgcctggca gtggcatatc ccggagctgg cccggcagat
     3721 ttacccgtgg gtcagctggc tgtaccggga gtattacagt ctggccggtt tttttctggt
     3781 tatcggcctg tttttcggtc tggctgaact ctgggccggc cgaatgcgtt ttgtggggct
     3841 gagccataag gcgcggcggg agtatgccga tcgctataag cgcgataata accggactgt
     3901 gccattttcg gtgctggcct cgctggattc acccgggcgg atggtcaggg cacggcgaca
     3961 tgtctggaac tacgcgtact ggctgaccgg cgctgccggt ctggcccttc tgggggggct
     4021 ggttgtcgcc gtgtttatcg agagctgggg agaaaccgtc ctgcgcccgg acctctatgc
     4081 gaacgcagat tattacctgc tgcagccggc taacctgttc gggatagcct ggtgtgccgg
     4141 agggcttgtc gccggcgtcc ttctggcgcg gctgctggcg ttcctgctgc ttgatggtca
     4201 ttttgcggcc gctgacgagg cggtgaacgc gaaactgaat caggagagcc ggcgcagcag
     4261 ccagcggacc ggagaaatga cagacgtccg ccacctgcat ttcggggagc cggtaccggt
     4321 taacgcgctg gccgattttt ccacggagca ggcccggaaa cagcaggcgg tgtttctggg
     4381 taaggatgag cagggccagc cggtgctggt ccctcgtgat acctggcgca agaccaatat
     4441 tcagatcctc ggcctgcccg ggagcggcaa gagcgcgatg gccaccaacg cgctcattcg
     4501 ctgcgtacgc gatttcgggg acgctgtggt gtacttcgac cccaaagggg acgcgtgggc
     4561 gccgcacgtc ttccgggcgc actgcccgga cttcaccctg ctggatctgc ggccggggaa
     4621 accggcgcag ctgaatctgt tccgcgatct cgaccagtac gccctgaaga atctgctggt
     4681 tgccgggttt aacctgagtg agaccggcga cgtggcggac cactaccgca tcagcgagca
     4741 gaaagcggcg aagctgattg cggagcagtt cccgcagggg gcaaacattc agcaggtgct
     4801 ggcggcggcg tacgcgttgc cggaggccct gaaaaaggac gtgaaggggc tgatcaccaa
     4861 gctggagaac gtggccgacc tgagcgtcct gcagacggac agcggtatcg atgtggcggg
     4921 gataatcaac ggcggcggct gcctgtacgt catcggctcg atggacgatg aggcggtgat
     4981 ccgggtgcag aagatgctgt ttgcccgctg cgcgcaaatc atcattgcgc gggatgagtt
     5041 ccggaggtgg ccgcacgcga gcatcatgct cgatgagatt aaatatctgc tgtcgaagta
     5101 cgtgctgaac gcgctgggga cgctgcgcag ccgggactgc aacctgctgc tcgcgcacca
     5161 gtcgctgggc gacttcggtc agtgcgggca ggatctgccc gctgattttg tgaaaaccac
     5221 ggtactggat aacacgccga tccgctggtt ctatcgcgcg gccagccagg agtccgcgca
     5281 gtgggcggcg ggccagaccg gggagatccg ggtggacgtg gagcgccgcc gggccagccg
     5341 ggaggcgggg aacgtggagc acatcagcgg cgacagcttc atccagaagg aggcgcggcc
     5401 gctgtttgac gtgaacaccc tgcaacacct gcctgacggg ttcgcggtga tgaccgggct
     5461 gggcgtggcg cggctggcgt tcagcagccc gctgcgggtt gagcgccggg aaatttcgct
     5521 gaaaaccttc ccggtgctgg cgaaaacgga cccgctggcg gagtacctgc ctccggccga
     5581 cggcggcgga ccaaaagcgg atgatgattt tgcggggctt tactgatgac actggagcag
     5641 tacaacgtca gccacgcgaa gcggcaggcg cggaacgcgg agaaaacacg gctgaccctg
     5701 cgctggctgc gggaagagct gtgttccacg gcggagctgg ttgcgcgacg gctggggatt
     5761 gcggcggtac agccggttta ccgcttcctg gacagtctgg tggcgaaagg gctgctggtc
     5821 cgggcgaaat acccggttga cggccgtcag gtcagcgtgt gggggctgac gccgcacggg
     5881 gtggcgttct cgttcgacga ggacgagccg ctgacggacg ttattccgtt tcagccgtca
     5941 agggtgtcgg cggcgcagct gccgcaccgg ctggccgtgc agtccctgcg cctctccatg
     6001 gagggccggg gggccaccgg ctggcggtac ctgcaccgta tggccctgaa agggatgaaa
     6061 gtgccggacg cgctggcgga gctggacggc agaacggtgg catttgaggt agagcgcacc
     6121 gtcaagagcc gcaggcggta tcaggaagtg gtgtccgggt atctgttcaa ccgccgggcg
     6181 aacggcatcg atgaaatctg gtacatctgc cctgaccggc cgacgcttgt ccgggtacag
     6241 cgggcgattc tggcggtgga tgaaatcgtg aatccacaga ccggcgaggc ccgcaaaacg
     6301 gtggagctgg accgggagag gctgttcgcg tgctttaaat ttatgacaac ggagtgaggg
     6361 attatggcgc tgtacatggt atttattgag gacgtggaag acaaagacgg cgagcgctgc
     6421 attatgccaa ccagcgagca tctggaagac atggtggtgc tcgcctggca gaccggggcg
     6481 tatgttttct gttttgtcga ccgggcccag catctggcga tgatgaaatc catcgggatg
     6541 tatgacgagc ggctcaacag cgacgaaaaa gccacgtact ttttcaccgg tgaaaatccg
     6601 caatgggaac gggacaaacc cacaggaatg aagctgtgtg attacctcta tcgaaaggtg
     6661 gagcggttcg ccatcgagaa cggtgtgcag gtggttgatg ccgggaagtg ctcaccggag
     6721 cagattaacg cgatgattga ggtggtcagc gagcggcacg gggaaagcgc ggtgaacgag
     6781 aagctgaaag cggagctgat gcgggaatat aacagccgct attttaacgt gccggtgcct
     6841 gaactgccgg aggacaaacc cgcccgccgg tggtggccgt tcggcaaaaa ataacgccag
     6901 caccggcatg gccggtgctg ttattcttcc ccgagcttat cccgcatcac ctgccagagt
     6961 gtttccgcat cctcgtgaag ccgttcgcac atgtcggccg gggtatccat gtccagctcg
     7021 ttcagctttt ccagcaacag cagcgactga tccttaatga atttagccat attgagggca
     7081 gttttttcct gcttcgtcat gttctccatt cccttcggta agttctcagc taaaccagcc
     7141 ggaccagaag cgttttttct tctgtgtcag ttctgttgtc aatctgtcca cttcggcctg
     7201 gagtttccgg cgctcatcat cgtgctggcg gcgttcttct tcccgggatg ttttatcttc
     7261 cagcatcagg gtgacagcct gccggagatc ggccagttcc ttttgcattg ccagaagctg
     7321 ttcctgtggg aaattttttt gtgtattttc acggtgtgaa atgccgggaa tttgtttctc
     7381 agggatattc acatttccgt atacacggat cagttctgac acatccacga cagggttgtt
     7441 tttcccgtca cgtgacacgg taacctttcc ctgttttaca tgattgtata gcgttcttct
     7501 ggtaatgccc gcggcttgtg ctgcctgtga aaggttgagc aatgttttcg ccactgtata
     7561 cccctgattt tatcgggtgt gaaaggtgtg taatttctca cggtaaatgt atcgctgaat
     7621 tttacccgtt tttccgttca ggatgtggtt ttctgacgcg ctgcgcttgt caaaccggcg
     7681 acggccacgc aatgaattgc gcccccgtca tcggttatta gaggctgtct gcccccggat
     7741 acccggattc tttctgatgc ctgacggtga cgatcacaat ctggtccatt tcccggtcgt
     7801 ggcggtagag catcacatag ccactgtcgc caaacccgat caccagttcc ttatattcca
     7861 gcggcagaaa cggcaccggg cggccaatgt ctggcaatgt tttcagttgc tggatagccc
     7921 ggacgatcac ctcacctcct tttctggctg ccagccgatt tttggttttg agaaagtcct
     7981 gaaggcgttg taaatcgcgc tgtgccagcg cggaaatcgt tacctgtggc atggcggagc
     8041 atcctgctcg ttctcagttc cccaggtact tatccaggct tccgcttctt ccgcagtaag
     8101 gtgcagaccc gtttcctgat agtgctgcca tgcggcctgt ccgtcccgca ggtactggct
     8161 gcgcttctct tcccggtcga tgtattcggt gattgcctcc agcattagcg cgtgcgctga
     8221 gcggtgccgg tcatcggcca gcgttttcag ccggtcctta agttcttcat cgagcctgat
     8281 ggatgttgct gatgacataa aagccccttt gtagtcatat gtattacaca tgactacttt
     8341 aatccgtggc ggcgctattt gccagaaata cggcttttgc tgcccatttt tcagcttcgc
     8401 ttccactaat cagggctgct acaggctcac tcatcatcca ggactattgc agcctgcggg
     8461 ttaaaggacc tgttactggc ggcaaccatc atcattgagc ttttgggtgg tgactggtcg
     8521 cggatcctgt aagccgtcat ttttcagact gaattcaaaa tttataacct gtcgcattgc
     8581 cgggtgccct gcgggatata gccagtgcct cattgccgtc agcgtttccc gaaaaaaaag
     8641 gtgacagccg gtaagcgaca cgttgtccac tcgtccatcg tccagaatat caaacgtggc
     8701 ggtcacctga ccttcgatgc gttttgctgc ggcctgtacc gggtaggttg gctgcagtgt
     8761 ggttacgggt tttagtggta tcgcatgtac cggagtgagg aacatggccg ccagcactgt
     8821 cagagtaaaa gctttcatgg tctgaaatcc agaggggcgt ttttgaatat ttgccaggta
     8881 ttttcatccg gtgccggcgg aattttggcg tgggtaacgg ctgatattgc tgctttacag
     8941 agtttcggat ctcctccttc agcaatggcg ctgttcagag tgccatcacg ttgtatggat
     9001 atccgcacgg tgcacatttt tcccgcatag gcctctggtt caaacatctg catttcaaca
     9061 gcggtcttga tggccgcagc gtagctactg attttagatg acttatccag agtataaatt
     9121 ttcggcggct gggtggtgga atgcttacag gccgtcagga gtaaggggct gcacaatgcc
     9181 agcaggataa agggtattaa acgcatcggg ttgatgacct gtcagtaaaa aatgttacat
     9241 gtatatttgt cactctttac ggccacgttc atttacataa acacggctta tcgtatcgtt
     9301 tttacaccaa acggcccgcc atgcagttgc gtgcataggc atgcagcggg ttacaattta
     9361 ccgggcgcgt ttccggcggt tttccgggtg gtttgttgcc tgttttatcc cgtagccgcc
     9421 ggaaacgccc tcagaccgtc tgagcggtgc gggcaatgcg acgttatggt aaatacccct
     9481 taccggcggc gctgtggctg ccaggtgaag gtggtttccg tagcctcgta tttctcccga
     9541 cgcatttcct gcgcccgcct ggcttcacgt tcgtcctgaa tggcacgaag caccctttcc
     9601 cgcacctgtt gttttaccgc ctttggcagc gttttgttcc ctttcaggcc catggccagt
     9661 gcctgagcga caaaggcgtc cgccaggcgc ttttcttcag cgggagtcag tttcaggtgc
     9721 gtcatagtgg cctccgggcg tctcaggttt cccgaacagt ttaaccgcag gtcctctcct
     9781 catgccagtt agctttcaga tgtgtctata ccatctgaaa tgtaagtgtg atgaatgtct
     9841 taaataataa aatttgttgc catgagaaat tttcgggaaa agacgcgatc tgcttacagg
     9901 tggctccggg cgcttttttg tgcctgaaga tgagaactaa tctcatatat tgacacctga
     9961 aaacgggagg agtaaggtaa taattatact gtgtatatgt acagtatgtt ggctttaatt
    10021 tattgtttta aatgtcaaag aggaaaacgg tatgagtgg
//
