LOCUS       NZ_CP147178            10060 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain SC35 plasmid p4, complete sequence.
ACCESSION   NZ_CP147178
VERSION     NZ_CP147178.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40144596
            Assembly: GCF_037157745.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Wang,D.
  TITLE     Klebsiella pneumoniae strains whole genome sequences
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Wang,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2024) School of Life Sciences, Taizhou University,
            1139 Shifudadao Avenue, Taizhou, Zhejiang 318000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.15.3; Canu v. 2.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina NovaSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037157745.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 18:29:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,675
            CDSs (total)                      :: 5,553
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 59 of 155
            Pseudo Genes (incomplete)         :: 105 of 155
            Pseudo Genes (internal stop)      :: 17 of 155
            Pseudo Genes (multiple problems)  :: 25 of 155
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147178.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SC35"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p4"
                     /geo_loc_name="China: Taizhou"
                     /lat_lon="28.40 N 121.26 E"
                     /collection_date="2021-04-19"
                     /collected_by="Dongguo Wang"
     gene            complement(join(9111..10060,1..1015))
                     /locus_tag="WBS25_RS28315"
     CDS             complement(join(9111..10060,1..1015))
                     /locus_tag="WBS25_RS28315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(2499..2648)
                     /locus_tag="WBS25_RS28320"
     CDS             complement(2499..2648)
                     /locus_tag="WBS25_RS28320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(2733..2990)
                     /locus_tag="WBS25_RS28325"
     CDS             complement(2733..2990)
                     /locus_tag="WBS25_RS28325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(3000..4685)
                     /locus_tag="WBS25_RS28330"
     CDS             complement(3000..4685)
                     /locus_tag="WBS25_RS28330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5012..5257
                     /locus_tag="WBS25_RS28335"
     CDS             5012..5257
                     /locus_tag="WBS25_RS28335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            5531..5902
                     /gene="tolA"
                     /locus_tag="WBS25_RS28340"
     CDS             5531..5902
                     /gene="tolA"
                     /locus_tag="WBS25_RS28340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            5899..6264
                     /locus_tag="WBS25_RS28345"
     CDS             5899..6264
                     /locus_tag="WBS25_RS28345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            6439..6717
                     /locus_tag="WBS25_RS28350"
     CDS             6439..6717
                     /locus_tag="WBS25_RS28350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            6705..7019
                     /locus_tag="WBS25_RS28355"
     CDS             6705..7019
                     /locus_tag="WBS25_RS28355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            7183..7611
                     /locus_tag="WBS25_RS28360"
     CDS             7183..7611
                     /locus_tag="WBS25_RS28360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            7637..7816
                     /locus_tag="WBS25_RS28365"
     CDS             7637..7816
                     /locus_tag="WBS25_RS28365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7843..8373)
                     /locus_tag="WBS25_RS28370"
     CDS             complement(7843..8373)
                     /locus_tag="WBS25_RS28370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8380..9111)
                     /gene="mobC"
                     /locus_tag="WBS25_RS28375"
     CDS             complement(8380..9111)
                     /gene="mobC"
                     /locus_tag="WBS25_RS28375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     BGC             1..9685
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1818..2396
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8384..9111)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 gcagattctt cagggcgtac tggtcgagat cgcggaacag attcagctgc gccggtttcc
       61 ccggccgcag atccagcagg gtgaagtccg ggcagtgcgc ccggaagacg tgcggcgccc
      121 acgcgtcccc tttggggtcg aagtacacca cagcgtcccc gaaatcgcgt acgcagcgaa
      181 tgagcgcgtt ggtggccatc gcgctcttgc cgctcccggg caggccgagg atctgaatat
      241 tggtcttgcg ccaggtatca cgagggacca gcaccggctg gccctgctca tccttaccca
      301 gaaacaccgc ctgctgtttc cgggcctgct ccgtggaaaa atcggccagc gcgttaaccg
      361 gtaccggctc cccgaaatgc aggtggcgga cgtctgtcat ttctccggtc cgctggctgc
      421 tgcgccggct ctcctgattc agtttcgcgt tcaccgcctc gtcagcggcc gcaaaatgac
      481 catcaagcag caggaacgcc agcagccgcg ccagaaggac gccggcgaca agccctccgg
      541 cacaccaggc tatcccgaac aggttagccg gctgcagcag gtaataatct gcgttcgcat
      601 agaggtccgg gcgcaggacg gtttctcccc agctctcgat aaacacggcg acaaccagcc
      661 cccccagaag ggccagaccg gcagcgccgg tcagccagta cgcgtagttc cagacatgtc
      721 gccgtgccct gaccatccgc ccgggtgaat ccagcgaggc cagcaccgaa aatggcacag
      781 tccggttatt atcgcgctta tagcgatcgg catactcccg ccgcgcctta tggctcagcc
      841 ccacaaaacg cattcggccg gcccagagtt cagccagacc gaaaaacagg ccgataacca
      901 gaaaaaaacc ggccagactg taatactccc ggtacagcca gctgacccac gggtaaatct
      961 gccgggccag ctccgggata tgccactgcc aggcaagcga atccgtatta aacatgcaat
     1021 ctctctcctt cagacgacgg gtttaccggc gtgatgcgca gcccccgctg cacaagccgc
     1081 ggacatgccc caaggcatgg cggcggcggt cacgtccgtg gtgattgcgt tcagctttca
     1141 ccgcggaggc cagcctcttt ccgccccatg cggaaaaggc gcagccccgg aggaacaacg
     1201 agcacgcccc agcgtgtgag aggaaaaggc ggcaccccag tgcggtcttt tccggtgttc
     1261 cggcccccgt cagagttcgc catagcgagc ggctgaccac ccgcaaaatc aggagcagct
     1321 ttgctgcttc gtcaggtgcg tccagcaccg tggatttttg tgggtggtca ggcgtgagcc
     1381 tgacgggggc cgggtgaatt tctggcgtgg ccggcgtcca gccggacaga gccagaaaga
     1441 ggggaaaaga ccgtgcgtcg gtggcgtcca gccagcgtaa gcaaggtctt ttcccctgaa
     1501 gtcagccacg ccccagcggg gatgacttgc atttctccgc aaggagaaaa aagcaacaga
     1561 gataggttaa cgtaaaatgt tcagcgaaaa accgacatgg ttcacctatc ctgataattg
     1621 atcgtcaggg aatagaaaga cttaatcagg ggacaatatc ccacatcagc gcgtccggtg
     1681 accgtcttgc aggtgccggg gaaattgcgc ctcctcgctc actcggtcgc tacgctccgg
     1741 gcgtgagact gcggcgggcg ctgcggacac atacaaagtt acccacagat tccgtggata
     1801 agcaggggac taacatgtga ggcaaaacgg cagggccgcg ccggtggcgt ttttccatag
     1861 gctccgccct cctgccagag ttcacataaa cagacgcttt ttcggtgcat ctgtgggagc
     1921 cgtgaggctc aaccatgaat ctgacagtac gggcgaaacc cgacaggact taaagatccc
     1981 caccgtttcc ggtaggtcgc tccctcttgc gctctcctgt tccgaccctg ccgtttaccg
     2041 gatacctgtc cgccgttctc ccttgcggga agtgtggcgc tttctcatag ctcacacact
     2101 ggtatctcgg ctcggtgtag gtcgttcgct ccaagctggg ctgtaagcaa gaactccccg
     2161 ttcagcccga ctgctgcgcc ttatccggta actgttcact tgagtccaac ccgggaaagc
     2221 acgcaaaacg ccactggcag cagccattgg taactgggag ttcgcagagg atttgtttag
     2281 ctaaacacgc ggttgctctt gaagtgtgcg ccaaagtccg gctacactgg aaggacagat
     2341 ttggttgctg tgctctgcga aagccagtta ccacggttaa gcagttcccc aactgactta
     2401 accttcgatc aaaccacctc cccaggtggt tttttcgttt acagggcaaa agattacgcg
     2461 cagaaaaaaa ggatctcaag aagatccttt gatcttttct actgaaccgc gatccccgtc
     2521 agttcagagg aggacgatgg tgccaccgtt ccgccctgaa catcccggat ataatttgcc
     2581 tgacatgcgg ccagaagaaa gccagcaaca atcaatataa aaagaaaaat agtctttacc
     2641 tttttcatat actttccctg tatttaaaga ctccggcatt catccctgaa caccggaagt
     2701 cagtatttca aatcatcctt gataattttt tgttaccagt taccgtcccg ataatcaaaa
     2761 gctatataac aattatccac atcaggtttt atttgatttt taaaaaatgg ctgtagtaaa
     2821 ggaacccatt ctttcttcaa atcaaactca ccattattaa tattatcctt taaaggcatt
     2881 cccaagcttt caatgacaga accatcgtca ccaaagtctt tagaatactc ttccccttta
     2941 aactcttcag ttttcttatc aaaccatgtc aaacgtaatt taagccccat atttacctct
     3001 caaagatatt ttttaatgtt ccgttttgga tctggccctt taacctgctt acccgttttg
     3061 ggatcaaatg cgcctaggtg ttcgccatca ctggcacgat acccttcaag ctcaccgtgc
     3121 tgggaatccc attcataaat tttacgtttt ttatcgccat accatcgggc ccgcttacca
     3181 ccaccacctt gtttaggtgt tttaggatca cctcgcttta actctccaag tccctttatg
     3241 tcttctgttt taggtactgg atgataatca tggccgtagt ctttagtccc cttgcgaggc
     3301 ttgttctttt cctcatttaa cttattttct gcatccttcg cttttttctc cttttgtttc
     3361 cggctctcca tcgccccggt aagtgcagcc ttatctctgg ataaggattc attcattgcg
     3421 tttaaagatg cctgagccgc ttttaatttc tcattctgat tctgaatatc cttatcaaga
     3481 tagcgaacct gatcctgcca cccggagcca tattcatgag gcgggttata agtcacaaaa
     3541 tcatctttat ttgcctctgc ctcttttacc tttttatcgg cttcctgaac agcagccctt
     3601 gcaccgggga gcgccttctc tgtcgatgca atgcggctgt ttaaggtcgc aatattttta
     3661 tcttcggcat ccagctcagc tttcgcttta tcatagtctc ttttcagccc ctcttccgga
     3721 tgagcggcgt cccatgcctg ctggcgacgc ttttcttcct cctgacgctg ttttacctgt
     3781 gccggggtaa gaacatctgt caccgacaca taaaccggct tctgtccggt ctctttcggg
     3841 aaacgaataa cggcctcacg ggagttacca ccggctgtaa aaccagccgg acgtgaatca
     3901 cctttttcag caacaatgcc ttttggcggg gctttcgctg tcggaacacc tttcggtacg
     3961 ctcacctgca gagacgggag acccggaatc gacacactga atacccccgg acgttttgtc
     4021 ggtttcgcat ccaccacagg gacactcatt ggccgacctg cgacaaccgc aatatgctgc
     4081 cgctcatcct tcaccacatc cacaacccgt tgtctgacac gaaccgtcgc ctggtccagt
     4141 ggtaaagtac ttgccggagt ctccgtcacc gcatcggccg gtaatgacgt cataattttt
     4201 gacatcattt tcggatcatc ttttgcaatc tcagaaggca gcacaccgta gatcgcaatc
     4261 ccccacagac caaacttaaa cggccccttc agggcagcca gcacatcagc aacggcggcc
     4321 gacaacgcat cgccggaaac tgataaagcc agtccttcag cccccggagt agccagggca
     4381 ggcagaccga aggccatcgc agtggccgaa gactgtccgc caccagagct accgctatga
     4441 ttgccgctgt taccaccatt accaccgggt ttactcccgc cgttaccaaa ctcccccgga
     4501 ttatagttat gaatatctcc attcggcgta tgcgtcgtac cccagcccgg cccgctgttt
     4561 ggatccgtac caccacctga tgaagggcca cctttaccag acgtaccgtt cacattccca
     4621 ctggcctgac caccattatg tcccagacct gaattacccg gacctcgacc gtctccacca
     4681 ctcataccgt tttcctcttt gacatttaaa acaataaatt aaagccaaca tactgtacat
     4741 atacacagta taattattac cttactcctc ccgttttcag gtgtcaatat atgagattag
     4801 ttctcatctt caggcacaaa aaagcgcccg gagccacctg taagcagatc gcgtcttttc
     4861 ccgaaaattt ctcatggcaa caaattttat tatttaagac attcatcaca cttacatttc
     4921 agatggtata gacacatctg aaagctaact ggcatgagga gaggacctgc ggttaaactg
     4981 ttcgggaaac ctgagacgcc cggaggccac tatgacgcac ctgaaactga ctcccgctga
     5041 agaaaagcgc ctggcggacg cctttgtcgc tcaggcactg gccatgggcc tgaaagggaa
     5101 caaaacgctg ccaaaggcgg taaaacaaca ggtgcgggaa agggtgcttc gtgccattca
     5161 ggacgaacgt gaagccaggc gggcgcagga aatgcgtcgg gagaaatacg aggctacgga
     5221 aaccaccttc acctggcagc cacagcgccg ccggtaaggg gtatttacca taacgtcgca
     5281 ttgcccgcac cgctcagacg gtctgagggc gtttccggcg gctacgggat aaaacaggca
     5341 acaaaccacc cggaaaaccg ccggaaacgc gcccggtaaa ttgtaacccg ctgcatgcct
     5401 atgcacgcaa ctgcatggcg ggccgtttgg tgtaaaaacg atacgataag ccgtgtttat
     5461 gtaaatgaac gtggccgtaa agagtgacaa atatacatgt aacatttttt actgacaggt
     5521 catcaacccg atgcgtttaa taccctttat cctgctggca ttgtgcagcc ccttactcct
     5581 gacggcctgt aagcattcca ccacccagcc gccgaaaatt tatactctgg ataagtcatc
     5641 taaaatcagt agctacgctg cggccatcaa gaccgctgtt gaaatgcaga tgtttgaacc
     5701 agaggcctat gcgggaaaaa tgtgcaccgt gcggatatcc atacaacgtg atggcactct
     5761 gaacagcgcc attgctgaag gaggagatcc gaaactctgt aaagcagcaa tatcagccgt
     5821 tacccacgcc aaaattccgc cggcaccgga tgaaaatacc tggcaaatat tcaaaaacgc
     5881 ccctctggat ttcagaccat gaaagctttt actctgacag tgctggcggc catgttcctc
     5941 actccggtac atgcgatacc actaaaaccc gtaaccacac tgcagccaac ctacccggta
     6001 caggccgcag caaaacgcat cgaaggtcag gtgaccgcca cgtttgatat tctggacgat
     6061 ggacgagtgg acaacgtgtc gcttaccggc tgtcaccttt tttttcggga aacgctgacg
     6121 gcaatgaggc actggctata tcccgcaggg cacccggcaa tgcgacaggt tataaatttt
     6181 gaattcagtc tgaaaaatga cggcttacag gatccgcgac cagtcaccac ccaaaagctc
     6241 aatgatgatg gttgccgcca gtaacaggtc ctttaacccg caggctgcaa tagtcctgga
     6301 tgatgagtga gcctgtagca gccctgatta gtggaagcga agctgaaaaa tgggcagcaa
     6361 aagccgtatt tctggcaaat agcgccgcca cggattaaag tagtcatgtg taatacatat
     6421 gactacaaag gggcttttat gtcatcagca acatccatca ggctcgatga agaacttaag
     6481 gaccggctga aaacgctggc cgatgaccgg caccgctcag cgcacgcgct aatgctggag
     6541 gcaatcaccg aatacatcga ccgggaagag aagcgcagcc agtacctgcg ggacggacag
     6601 gccgcatggc agcactatca ggaaacgggt ctgcacctta ctgcggaaga agcggaagcc
     6661 tggataagta cctggggaac tgagaacgag caggatgctc cgccatgcca caggtaacga
     6721 tttccgcgct ggcacagcgc gatttacaac gccttcagga ctttctcaaa accaaaaatc
     6781 ggctggcagc cagaaaagga ggtgaggtga tcgtccgggc tatccagcaa ctgaaaacat
     6841 tgccagacat tggccgcccg gtgccgtttc tgccgctgga atataaggaa ctggtgatcg
     6901 ggtttggcga cagtggctat gtgatgctct accgccacga ccgggaaatg gaccagattg
     6961 tgatcgtcac cgtcaggcat cagaaagaat ccgggtatcc gggggcagac agcctctaat
     7021 aaccgatgac gggggcgcaa ttcattgcgt ggccgtcgcc ggtttgacaa gcgcagcgcg
     7081 tcagaaaacc acatcctgaa cggaaaaacg ggtaaaattc agcgatacat ttaccgtgag
     7141 aaattacaca cctttcacac ccgataaaat caggggtata cagtggcgaa aacattgctc
     7201 aacctttcac aggcagcaca agccgcgggc attaccagaa gaacgctata caatcatgta
     7261 aaacagggaa aggttaccgt gtcacgtgac gggaaaaaca accctgtcgt ggatgtgtca
     7321 gaactgatcc gtgtatacgg aaatgtgaat atccctgaga aacaaattcc cggcatttca
     7381 caccgtgaaa atacacaaaa aaatttccca caggaacagc ttctggcaat gcaaaaggaa
     7441 ctggccgatc tccggcaggc tgtcaccctg atgctggaag ataaaacatc ccgggaagaa
     7501 gaacgccgcc agcacgatga tgagcgccgg aaactccagg ccgaagtgga cagattgaca
     7561 acagaactga cacagaagaa aaaacgcttc tggtccggct ggtttagctg agaacttacc
     7621 gaagggaatg gagaacatga cgaagcagga aaaaactgcc ctcaatatgg ctaaattcat
     7681 taaggatcag tcgctgctgt tgctggaaaa gctgaacgag ctggacatgg ataccccggc
     7741 cgacatgtgc gaacggcttc acgaggatgc ggaaacactc tggcaggtga tgcgggataa
     7801 gctcggggaa gaataacagc accggccatg ccggtgctgg cgttattttt tgccgaacgg
     7861 ccaccaccgg cgggcgggtt tgtcctccgg cagttcaggc accggcacgt taaaatagcg
     7921 gctgttatat tcccgcatca gctccgcttt cagcttctcg ttcaccgcgc tttccccgtg
     7981 ccgctcgctg accacctcaa tcatcgcgtt aatctgctcc ggtgagcact tcccggcatc
     8041 aaccacctgc acaccgttct cgatggcgaa ccgctccacc tttcgataga ggtaatcaca
     8101 cagcttcatt cctgtgggtt tgtcccgttc ccattgcgga ttttcaccgg tgaaaaagta
     8161 cgtggctttt tcgtcgctgt tgagccgctc gtcatacatc ccgatggatt tcatcatcgc
     8221 cagatgctgg gcccggtcga caaaacagaa aacatacgcc ccggtctgcc aggcgagcac
     8281 caccatgtct tccagatgct cgctggttgg cataatgcag cgctcgccgt ctttgtcttc
     8341 cacgtcctca ataaatacca tgtacagcgc cataatccct cactccgttg tcataaattt
     8401 aaagcacgcg aacagcctct cccggtccag ctccaccgtt ttgcgggcct cgccggtctg
     8461 tggattcacg atttcatcca ccgccagaat cgcccgctgt acccggacaa gcgtcggccg
     8521 gtcagggcag atgtaccaga tttcatcgat gccgttcgcc cggcggttga acagataccc
     8581 ggacaccact tcctgatacc gcctgcggct cttgacggtg cgctctacct caaatgccac
     8641 cgttctgccg tccagctccg ccagcgcgtc cggcactttc atccctttca gggccatacg
     8701 gtgcaggtac cgccagccgg tggccccccg gccctccatg gagaggcgca gggactgcac
     8761 ggccagccgg tgcggcagct gcgccgccga cacccttgac ggctgaaacg gaataacgtc
     8821 cgtcagcggc tcgtcctcgt cgaacgagaa cgccaccccg tgcggcgtca gcccccacac
     8881 gctgacctga cggccgtcaa ccgggtattt cgcccggacc agcagccctt tcgccaccag
     8941 actgtccagg aagcggtaaa ccggctgtac cgccgcaatc cccagccgtc gcgcaaccag
     9001 ctccgccgtg gaacacagct cttcccgcag ccagcgcagg gtcagccgtg ttttctccgc
     9061 gttccgcgcc tgccgcttcg cgtggctgac gttgtactgc tccagtgtca tcagtaaagc
     9121 cccgcaaaat catcatccgc ttttggtccg ccgccgtcgg ccggaggcag gtactccgcc
     9181 agcgggtccg ttttcgccag caccgggaag gttttcagcg aaatttcccg gcgctcaacc
     9241 cgcagcgggc tgctgaacgc cagccgcgcc acgcccagcc cggtcatcac cgcgaacccg
     9301 tcaggcaggt gttgcagggt gttcacgtca aacagcggcc gcgcctcctt ctggatgaag
     9361 ctgtcgccgc tgatgtgctc cacgttcccc gcctcccggc tggcccggcg gcgctccacg
     9421 tccacccgga tctccccggt ctggcccgcc gcccactgcg cggactcctg gctggccgcg
     9481 cgatagaacc agcggatcgg cgtgttatcc agtaccgtgg ttttcacaaa atcagcgggc
     9541 agatcctgcc cgcactgacc gaagtcgccc agcgactggt gcgcgagcag caggttgcag
     9601 tcccggctgc gcagcgtccc cagcgcgttc agcacgtact tcgacagcag atatttaatc
     9661 tcatcgagca tgatgctcgc gtgcggccac ctccggaact catcccgcgc aatgatgatt
     9721 tgcgcgcagc gggcaaacag catcttctgc acccggatca ccgcctcatc gtccatcgag
     9781 ccgatgacgt acaggcagcc gccgccgttg attatccccg ccacatcgat accgctgtcc
     9841 gtctgcagga cgctcaggtc ggccacgttc tccagcttgg tgatcagccc cttcacgtcc
     9901 tttttcaggg cctccggcaa cgcgtacgcc gccgccagca cctgctgaat gtttgccccc
     9961 tgcgggaact gctccgcaat cagcttcgcc gctttctgct cgctgatgcg gtagtggtcc
    10021 gccacgtcgc cggtctcact caggttaaac ccggcaacca
//
