LOCUS       NZ_CP147173             5596 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain SC38 plasmid p4, complete sequence.
ACCESSION   NZ_CP147173
VERSION     NZ_CP147173.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40144597
            Assembly: GCF_037157735.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Wang,D.
  TITLE     Klebsiella pneumoniae strains whole genome sequences
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Wang,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2024) School of Life Sciences, Taizhou University,
            1139 Shifudadao Avenue, Taizhou, Zhejiang 318000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.15.3; Canu v. 2.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina NovaSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037157735.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 18:35:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,830
            CDSs (total)                      :: 5,709
            Genes (coding)                    :: 5,534
            CDSs (with protein)               :: 5,534
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 60 of 175
            Pseudo Genes (incomplete)         :: 117 of 175
            Pseudo Genes (internal stop)      :: 18 of 175
            Pseudo Genes (multiple problems)  :: 19 of 175
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147173.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SC38"
                     /isolation_source="clean catch midstream urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p4"
                     /geo_loc_name="China: Taizhou"
                     /lat_lon="28.40 N 121.26 E"
                     /collection_date="2021-07-08"
                     /collected_by="Dongguo Wang"
     gene            529..753
                     /locus_tag="WBS26_RS29100"
     CDS             529..753
                     /locus_tag="WBS26_RS29100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            737..1003
                     /locus_tag="WBS26_RS29105"
     CDS             737..1003
                     /locus_tag="WBS26_RS29105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1073..1423)
                     /locus_tag="WBS26_RS29110"
     CDS             complement(1073..1423)
                     /locus_tag="WBS26_RS29110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(1489..2034)
                     /locus_tag="WBS26_RS29115"
     CDS             complement(1489..2034)
                     /locus_tag="WBS26_RS29115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(2060..2386)
                     /locus_tag="WBS26_RS29120"
     CDS             complement(2060..2386)
                     /locus_tag="WBS26_RS29120"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(2744..2902)
                     /locus_tag="WBS26_RS29125"
     CDS             complement(2744..2902)
                     /locus_tag="WBS26_RS29125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            3542..3718
                     /locus_tag="WBS26_RS29130"
     CDS             3542..3718
                     /locus_tag="WBS26_RS29130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            3708..4052
                     /locus_tag="WBS26_RS29135"
     CDS             3708..4052
                     /locus_tag="WBS26_RS29135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4261..>4695
                     /locus_tag="WBS26_RS29140"
                     /pseudo=""
     CDS             4261..>4695
                     /locus_tag="WBS26_RS29140"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4730..5239
                     /locus_tag="WBS26_RS29145"
     CDS             4730..5239
                     /locus_tag="WBS26_RS29145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            5246..5458
                     /locus_tag="WBS26_RS29150"
     CDS             5246..5458
                     /locus_tag="WBS26_RS29150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          3377..3456
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(2794..3191)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          4043..5574
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctcatcgggc atttacgctg aaaccggatg tcattcacct ggctgcatgg ctatgcagcc
       61 aggtaaaatt tatctgtcgc gaacggggcg gtttatcggg tggtttgttg ccggttttac
      121 ctgactgcca ccccgttcgc ggcgaacccg tccggggcgg tgcgggcaac gggtgttatg
      181 tttaatcagc gtgaacggca gatacaaaaa agccccggag ggcttttaac tactgactta
      241 tttttttgaa gatttgctgg gtttcaattt gtcagtattt cccggcgttt ttttattgtc
      301 acgctggcgt ttatcaggtt cccctgttga atcggcaatt cgtttcggac ccggcttaag
      361 acccataatc gttatccttt caatagttaa gaggaaagcc ctcaagctag atatattgac
      421 cgcgttacgg attatcaaga ggggctaaca agattatttt ttgttctttt aggttgattg
      481 tggtactgtt gatatacatg tatagcaaat ggtgttcggg aggtttatat gcttgctatc
      541 cggttatctg atgagattga gtcccgtctg gactcgctgg cgaagcaaac cggcagaaca
      601 aagacgtttt atgcgcggga agcaatactg gcgcatctgg aagacctgga ggattattat
      661 ctttcagcag aaactgctgc acgcgttcgc cgtggtgatg aagcagtgca ttcgtctgaa
      721 gacgtgagga agtcacttgg tctggacgat taactattcc gatcgggcgc tcaaatcgtt
      781 acgcaagatg gacaaacaga acgcacgacg gattgtggat tttatgagtt tacgcattgc
      841 agttgctgcc gatcctcgcc agtcagggaa gccgctcaaa ggtgagctgg gcgagttctg
      901 gcgctatcgg gtgggagatt atcgcgttct gtgtgagatc cgagatgacg agcttgttat
      961 ccttgccgcc acgattggac atcgccgcga agtttacgac tgaagcccgc tatgcgggct
     1021 tttttgatgc cgacaacagg tattatggta aatactcgac taactacgtg ttctactgtt
     1081 tgtcggtttt agttaaccag caggttgtct taacgataat gtagccgtta tttttaacta
     1141 ataaggtcat attgttaacg tcaggcataa taaaagacat tatggcctca gagttatcat
     1201 cagaagcagc tctctgcatt actacttcga ttcccttttc tttgaatcta taagtatcca
     1261 gttgcctatt aaacttagaa agacctacaa ccatacttag tttgttatct ttaacagtaa
     1321 gagatgcttc ctgagtacca ttacatcggt agtaagcggt gtaaccgtca gcaaaagatc
     1381 gaccggggaa tataaatgct aggaacgcaa ttaaccaata catagttgag aatcttggta
     1441 ttacacattt cactatcctc tctttcattt ttcgttcacc atactttgtt atctgatttt
     1501 taatttttct attttataaa ccaagtctct accaaaaagg cgaaaattat cctcaaataa
     1561 taataattgt ttaagaacat ccatgaaagc agctgcttca ccctcagtta tgggagctac
     1621 tctatataac atataacctg agttaagggt ttcttttata ctttcttcaa tgctcattat
     1681 aaatattagt ggattatcat ttttcttccc ccatgggttt ctaattaatc cttcgatgta
     1741 ctcaggtcct ttgcgtccta ttgcctgcgc aatatttttt atttcatctg cgtgagggct
     1801 ttgttgtaca gctttgttta tgcctgcttg agtgattttc agagactcta caccgtgtat
     1861 gttgtatgag ataaaatgta ataaattaag atgcaatgca attaatccat ttttgtttac
     1921 atctgagtat ttggataaaa aacgtaatga agtgttaact ctcccttgaa gcctaacaat
     1981 tgggagtgct gccttgtata tttccttctc cgttaaatac cgtttaaaaa acataattct
     2041 ccgcctccgt atagcgtgcc tattttattt cgtcgtaaaa cttagctctg aaatattcct
     2101 ttagaaccat catgtcatta ataattgaat ctatctcatc cattttttct tggtattctt
     2161 ttaatggtga agggagcggt aactcaatat ttgaaaagtg attggcgaga tagggatcaa
     2221 ggtttctcat ccattcaggc ttttcgccaa tgcgttcttc aactgaaagt gaacctgata
     2281 atctctggtt aataagatca cttagactca ttttggtttt attcgaaagc cgttgtaagg
     2341 attgctttaa tcctatatct agcatgatat ttgttctttc tctcattacc cccccttgct
     2401 attgcgtttt ttgttgcgca caaagctacc attgcgcatt aagaaacgca ataaaaaagg
     2461 agggagggag tggtttgtac atgatgtgca gtgcgctgag ggcatcagtg gcgggtgttt
     2521 ttccgtggga tggatggagc tccggggata acagaactga cgatcattta aaggatcttc
     2581 ttgagatcct ttttttctgc gcgtaatctc ttgcactgta aacgaaaaaa ccgcctgggg
     2641 aggcggtttg atcgaaggtt aagtcagttg gggaactgct taaccgggta actgggtata
     2701 cgagaccgca gataccaaaa ctgttctttc agtgtagccg cagttaggca cccacttcaa
     2761 gacctctcaa tatctctctc gtatatatcc agttaccaat ggctgctgcc agtggcgttt
     2821 ttgcgtgtcc gtccgggttg gactcaagat gatagttacc ggaaagggcg cagcagtcgg
     2881 gctgaacggg gggttcgtgc atacagccca gcttggagcg aacgacctaa accgagccga
     2941 gataccagca gcgtgagcta tgagaaagcg ccacgcttcc cgaaagggaa aaaggcggaa
     3001 caggtatccg gtaaacggca gggtcggaac aggagagcgc acgagggagc cgccggagag
     3061 aaatcaccgg catctttaag tcctgtcggg tttcgtcctc ctctgacttg agcgtccatt
     3121 tctgtgatgc tcgtcagggg aggcggagcc tatggaaaaa cggcaacgcc gcatcctttt
     3181 ctccggccct ttctgatttg agcgcctttg ctttatcccg ttatccgtga gtcggtcacg
     3241 cccgccgcag ccgaacggca ggagcgcagc taccgagtga gcgaggaggc gtcatttcat
     3301 ctgtccgtgc atggtattgc ggacgctcct gagccacata tcacacctgc cacatgaggc
     3361 actttcatga atcgtgctct catgccaaca tagtaagcca gtatacacag cgtaaacgct
     3421 gtgactggtt cagggctgcg ccccgaaacc cgctaacacc actgacgcgc tggcgcttgt
     3481 ccaacgccgg agcgacccgg aaaacttttc cgtgccgtcc ggcgtttgcg aaggagggat
     3541 aatgcagaag ccgaagaaga agccgctcgt cgtaaaaaaa agatcctggc atccggcagt
     3601 gatggcaaat ctgatgcgtc aggcacagga ggaagattac cgccgggagc agatggcaga
     3661 gcttgagcgt tcagcccggt cacattttgc ggatatcgga ggagaggatg gcggttaagc
     3721 gtacccgttt tttaggtatc cgggtgaccg atggggagta ccagcagctg ctggagcgct
     3781 gcaatggaag gcaactggcg gtgtggatgc gggaaacctg tctcgatacg cgcccggcgc
     3841 ggtcgttgcg gctgccgtcc atcgatccgg ttttgctgcg ccagcttgcc gggatgggga
     3901 acaacctcaa ccagatcgcc cggaaaatta acggcggcca gtggtcaggc gctgaccggg
     3961 ttcaggtggt ggccgcgctg atggccattg atgccgaact cgagcggctg cggcatgccg
     4021 tgctggaaaa gggggcagac gatgatcgtt aagtttcatc cgcgagggcg aggcggcggt
     4081 gccgggccgg tggattatct gctggggaaa gaccgccagc gcgacggggc catcgtcctg
     4141 cagggaaagc cggaggaggt ccgggagctt atcgacgcct cgccctacgc caaaaagtac
     4201 acctccggcg tcctgtcttt tgctgaacag gatttaccgc ccggccagcg tgaaaagctg
     4261 atggcgagct tcgagcgggt tctgatgccc ggactcgata aagaccagta cagcgtgctg
     4321 tgggttgaac accgggacaa ggggcggctg gaactgaatt ttctggtacc gaacacggaa
     4381 ctgctgaccg gcaggcgtct ccagccgtac tacgaccggg cagaccgtcc gcgcatcgat
     4441 gcgtggcaga ccgtggtgaa cgggcgactg gggctgcacg acccgaacgc accggagaac
     4501 cggcgcgcgc tggtgacgcc atcttcgtta cccaaaacga agcaggaagc cgcagaggct
     4561 attacgcggg gcttactggc cctcgcctcg tcaggggagc ttaaaacgcg tcaggacgtc
     4621 actgaggcgc tggaaagcgc aggttttgag gtcgtgcgca ccacaaagag cagcatcagc
     4681 attgccgacc cggacggagg gcgaaacatc cgacttaagg gagccatcta tgaacagtct
     4741 tttaacgctg gcgaaggact tagagcagaa atcgaaagcg cagcagcaga gtaccggcga
     4801 aatgctgaaa gccgcattca gcgagcacga gaagtctgtc agagcggaac tgagcgaaag
     4861 cgggaagaga atcagcgccg ccatccgcgc ccacgagcag ggtatgagcg cggccatgca
     4921 gtcgaaccgc ctgagcgtga tgcgtatggt cgggcggaca tggctgacca tcacgatggt
     4981 gtcaggactg ctgttcgcca gcctgagcgg ggtgttgtgg tatcagggga gcttgatagc
     5041 gtcaaatctg gccgaaatcg accggcagaa cgcagcgctg tcgaagctga acgcgaagac
     5101 ctggggcgtg acgtacctgg aagacagcaa cggacgtttt ctggtgctgc cgaaggggac
     5161 ggccgtagac aggacgcaga gctggacggt cgggaacggg cggtcgaagc agaacgcgct
     5221 gagactggtg aaggagtaag tgccgatgac cgagctggaa aaacagttgc tgagcgcatt
     5281 agagcagcta cagcaggact actcgaaaag gctggacgag tgggagagcg ccttcgggga
     5341 atggcggaaa atgtcagggc ttatgcaacg ggagaacgcg gcgctgagcg agcgcgtgac
     5401 gcgcttgagt cagcaggtgg aacgcttgag cgggcaactg cagcgtttga gccggtagtg
     5461 cagcgtcatg agatggccgt ggccgcagaa cgggcgcacg agcaacggca gcacgaaaag
     5521 gaactgacgg aagcgcgttc ccggcagcga agctatgacg gtccgtcgct gggctgaaat
     5581 gcaggaaaac agggtt
//
