LOCUS       NZ_CP147172            10060 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain SC38 plasmid p3, complete sequence.
ACCESSION   NZ_CP147172
VERSION     NZ_CP147172.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40144597
            Assembly: GCF_037157735.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Wang,D.
  TITLE     Klebsiella pneumoniae strains whole genome sequences
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Wang,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2024) School of Life Sciences, Taizhou University,
            1139 Shifudadao Avenue, Taizhou, Zhejiang 318000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.15.3; Canu v. 2.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina NovaSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037157735.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 18:35:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,830
            CDSs (total)                      :: 5,709
            Genes (coding)                    :: 5,534
            CDSs (with protein)               :: 5,534
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 60 of 175
            Pseudo Genes (incomplete)         :: 117 of 175
            Pseudo Genes (internal stop)      :: 18 of 175
            Pseudo Genes (multiple problems)  :: 19 of 175
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147172.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SC38"
                     /isolation_source="clean catch midstream urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p3"
                     /geo_loc_name="China: Taizhou"
                     /lat_lon="28.40 N 121.26 E"
                     /collection_date="2021-07-08"
                     /collected_by="Dongguo Wang"
     gene            complement(join(9572..10060,1..42))
                     /locus_tag="WBS26_RS29035"
     CDS             complement(join(9572..10060,1..42))
                     /locus_tag="WBS26_RS29035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(49..780)
                     /gene="mobC"
                     /locus_tag="WBS26_RS29040"
     CDS             complement(49..780)
                     /gene="mobC"
                     /locus_tag="WBS26_RS29040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(780..2744)
                     /locus_tag="WBS26_RS29045"
     CDS             complement(780..2744)
                     /locus_tag="WBS26_RS29045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(4228..4377)
                     /locus_tag="WBS26_RS29050"
     CDS             complement(4228..4377)
                     /locus_tag="WBS26_RS29050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(4462..4719)
                     /locus_tag="WBS26_RS29055"
     CDS             complement(4462..4719)
                     /locus_tag="WBS26_RS29055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(4729..6414)
                     /locus_tag="WBS26_RS29060"
     CDS             complement(4729..6414)
                     /locus_tag="WBS26_RS29060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            6741..6986
                     /locus_tag="WBS26_RS29065"
     CDS             6741..6986
                     /locus_tag="WBS26_RS29065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            7260..7631
                     /gene="tolA"
                     /locus_tag="WBS26_RS29070"
     CDS             7260..7631
                     /gene="tolA"
                     /locus_tag="WBS26_RS29070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            7628..7993
                     /locus_tag="WBS26_RS29075"
     CDS             7628..7993
                     /locus_tag="WBS26_RS29075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            8168..8446
                     /locus_tag="WBS26_RS29080"
     CDS             8168..8446
                     /locus_tag="WBS26_RS29080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            8434..8748
                     /locus_tag="WBS26_RS29085"
     CDS             8434..8748
                     /locus_tag="WBS26_RS29085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            8912..9340
                     /locus_tag="WBS26_RS29090"
     CDS             8912..9340
                     /locus_tag="WBS26_RS29090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            9366..9545
                     /locus_tag="WBS26_RS29095"
     CDS             9366..9545
                     /locus_tag="WBS26_RS29095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     BGC             1..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3547..4125
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(53..780)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 tttgtcttcc acgtcctcaa taaataccat gtacagcgcc ataatccctc actccgttgt
       61 cataaattta aagcacgcga acagcctctc ccggtccagc tccaccgttt tgcgggcctc
      121 gccggtctgt ggattcacga tttcatccac cgccagaatc gcccgctgta cccggacaag
      181 cgtcggccgg tcagggcaga tgtaccagat ttcatcgatg ccgttcgccc ggcggttgaa
      241 cagatacccg gacaccactt cctgataccg cctgcggctc ttgacggtgc gctctacctc
      301 aaatgccacc gttctgccgt ccagctccgc cagcgcgtcc ggcactttca tccctttcag
      361 ggccatacgg tgcaggtacc gccagccggt ggccccccgg ccctccatgg agaggcgcag
      421 ggactgcacg gccagccggt gcggcagctg cgccgccgac acccttgacg gctgaaacgg
      481 aataacgtcc gtcagcggct cgtcctcgtc gaacgagaac gccaccccgt gcggcgtcag
      541 cccccacacg ctgacctgac ggccgtcaac cgggtatttc gcccggacca gcagcccttt
      601 cgccaccaga ctgtccagga agcggtaaac cggctgtacc gccgcaatcc ccagccgtcg
      661 cgcaaccagc tccgccgtgg aacacagctc ttcccgcagc cagcgcaggg tcagccgtgt
      721 tttctccgcg ttccgcgcct gccgcttcgc gtggctgacg ttgtactgct ccagtgtcat
      781 cagtaaagcc ccgcaaaatc atcatccgct tttggtccgc cgccgtcggc cggaggcagg
      841 tactccgcca gcgggtccgt tttcgccagc accgggaagg ttttcagcga aatttcccgg
      901 cgctcaaccc gcagcgggct gctgaacgcc agccgcgcca cgcccagccc ggtcatcacc
      961 gcgaacccgt caggcaggtg ttgcagggtg ttcacgtcaa acagcggccg cgcctccttc
     1021 tggatgaagc tgtcgccgct gatgtgctcc acgttccccg cctcccggct ggcccggcgg
     1081 cgctccacgt ccacccggat ctccccggtc tggcccgccg cccactgcgc ggactcctgg
     1141 ctggccgcgc gatagaacca gcggatcggc gtgttatcca gtaccgtggt tttcacaaaa
     1201 tcagcgggca gatcctgccc gcactgaccg aagtcgccca gcgactggtg cgcgagcagc
     1261 aggttgcagt cccggctgcg cagcgtcccc agcgcgttca gcacgtactt cgacagcaga
     1321 tatttaatct catcgagcat gatgctcgcg tgcggccacc tccggaactc atcccgcgca
     1381 atgatgattt gcgcgcagcg ggcaaacagc atcttctgca cccggatcac cgcctcatcg
     1441 tccatcgagc cgatgacgta caggcagccg ccgccgttga ttatccccgc cacatcgata
     1501 ccgctgtccg tctgcaggac gctcaggtcg gccacgttct ccagcttggt gatcagcccc
     1561 ttcacgtcct ttttcagggc ctccggcaac gcgtacgccg ccgccagcac ctgctgaatg
     1621 tttgccccct gcgggaactg ctccgcaatc agcttcgccg ctttctgctc gctgatgcgg
     1681 tagtggtccg ccacgtcgcc ggtctcactc aggttaaacc cggcaaccag cagattcttc
     1741 agggcgtact ggtcgagatc gcggaacaga ttcagctgcg ccggtttccc cggccgcaga
     1801 tccagcaggg tgaagtccgg gcagtgcgcc cggaagacgt gcggcgccca cgcgtcccct
     1861 ttggggtcga agtacaccac agcgtccccg aaatcgcgta cgcagcgaat gagcgcgttg
     1921 gtggccatcg cgctcttgcc gctcccgggc aggccgagga tctgaatatt ggtcttgcgc
     1981 caggtatcac gagggaccag caccggctgg ccctgctcat ccttacccag aaacaccgcc
     2041 tgctgtttcc gggcctgctc cgtggaaaaa tcggccagcg cgttaaccgg taccggctcc
     2101 ccgaaatgca ggtggcggac gtctgtcatt tctccggtcc gctggctgct gcgccggctc
     2161 tcctgattca gtttcgcgtt caccgcctcg tcagcggccg caaaatgacc atcaagcagc
     2221 aggaacgcca gcagccgcgc cagaaggacg ccggcgacaa gccctccggc acaccaggct
     2281 atcccgaaca ggttagccgg ctgcagcagg taataatctg cgttcgcata gaggtccggg
     2341 cgcaggacgg tttctcccca gctctcgata aacacggcga caaccagccc ccccagaagg
     2401 gccagaccgg cagcgccggt cagccagtac gcgtagttcc agacatgtcg ccgtgccctg
     2461 accatccgcc cgggtgaatc cagcgaggcc agcaccgaaa atggcacagt ccggttatta
     2521 tcgcgcttat agcgatcggc atactcccgc cgcgccttat ggctcagccc cacaaaacgc
     2581 attcggccgg cccagagttc agccagaccg aaaaacaggc cgataaccag aaaaaaaccg
     2641 gccagactgt aatactcccg gtacagccag ctgacccacg ggtaaatctg ccgggccagc
     2701 tccgggatat gccactgcca ggcaagcgaa tccgtattaa acatgcaatc tctctccttc
     2761 agacgacggg tttaccggcg tgatgcgcag cccccgctgc acaagccgcg gacatgcccc
     2821 aaggcatggc ggcggcggtc acgtccgtgg tgattgcgtt cagctttcac cgcggaggcc
     2881 agcctctttc cgccccatgc ggaaaaggcg cagccccgga ggaacaacga gcacgcccca
     2941 gcgtgtgaga ggaaaaggcg gcaccccagt gcggtctttt ccggtgttcc ggcccccgtc
     3001 agagttcgcc atagcgagcg gctgaccacc cgcaaaatca ggagcagctt tgctgcttcg
     3061 tcaggtgcgt ccagcaccgt ggatttttgt gggtggtcag gcgtgagcct gacgggggcc
     3121 gggtgaattt ctggcgtggc cggcgtccag ccggacagag ccagaaagag gggaaaagac
     3181 cgtgcgtcgg tggcgtccag ccagcgtaag caaggtcttt tcccctgaag tcagccacgc
     3241 cccagcgggg atgacttgca tttctccgca aggagaaaaa agcaacagag ataggttaac
     3301 gtaaaatgtt cagcgaaaaa ccgacatggt tcacctatcc tgataattga tcgtcaggga
     3361 atagaaagac ttaatcaggg gacaatatcc cacatcagcg cgtccggtga ccgtcttgca
     3421 ggtgccgggg aaattgcgcc tcctcgctca ctcggtcgct acgctccggg cgtgagactg
     3481 cggcgggcgc tgcggacaca tacaaagtta cccacagatt ccgtggataa gcaggggact
     3541 aacatgtgag gcaaaacggc agggccgcgc cggtggcgtt tttccatagg ctccgccctc
     3601 ctgccagagt tcacataaac agacgctttt tcggtgcatc tgtgggagcc gtgaggctca
     3661 accatgaatc tgacagtacg ggcgaaaccc gacaggactt aaagatcccc accgtttccg
     3721 gtaggtcgct ccctcttgcg ctctcctgtt ccgaccctgc cgtttaccgg atacctgtcc
     3781 gccgttctcc cttgcgggaa gtgtggcgct ttctcatagc tcacacactg gtatctcggc
     3841 tcggtgtagg tcgttcgctc caagctgggc tgtaagcaag aactccccgt tcagcccgac
     3901 tgctgcgcct tatccggtaa ctgttcactt gagtccaacc cgggaaagca cgcaaaacgc
     3961 cactggcagc agccattggt aactgggagt tcgcagagga tttgtttagc taaacacgcg
     4021 gttgctcttg aagtgtgcgc caaagtccgg ctacactgga aggacagatt tggttgctgt
     4081 gctctgcgaa agccagttac cacggttaag cagttcccca actgacttaa ccttcgatca
     4141 aaccacctcc ccaggtggtt ttttcgttta cagggcaaaa gattacgcgc agaaaaaaag
     4201 gatctcaaga agatcctttg atcttttcta ctgaaccgcg atccccgtca gttcagagga
     4261 ggacgatggt gccaccgttc cgccctgaac atcccggata taatttgcct gacatgcggc
     4321 cagaagaaag ccagcaacaa tcaatataaa aagaaaaata gtctttacct ttttcatata
     4381 ctttccctgt atttaaagac tccggcattc atccctgaac accggaagtc agtatttcaa
     4441 atcatccttg ataatttttt gttaccagtt accgtcccga taatcaaaag ctatataaca
     4501 attatccaca tcaggtttta tttgattttt aaaaaatggc tgtagtaaag gaacccattc
     4561 tttcttcaaa tcaaactcac cattattaat attatccttt aaaggcattc ccaagctttc
     4621 aatgacagaa ccatcgtcac caaagtcttt agaatactct tcccctttaa actcttcagt
     4681 tttcttatca aaccatgtca aacgtaattt aagccccata tttacctctc aaagatattt
     4741 tttaatgttc cgttttggat ctggcccttt aacctgctta cccgttttgg gatcaaatgc
     4801 gcctaggtgt tcgccatcac tggcacgata cccttcaagc tcaccgtgct gggaatccca
     4861 ttcataaatt ttacgttttt tatcgccata ccatcgggcc cgcttaccac caccaccttg
     4921 tttaggtgtt ttaggatcac ctcgctttaa ctctccaagt ccctttatgt cttctgtttt
     4981 aggtactgga tgataatcat ggccgtagtc tttagtcccc ttgcgaggct tgttcttttc
     5041 ctcatttaac ttattttctg catccttcgc ttttttctcc ttttgtttcc ggctctccat
     5101 cgccccggta agtgcagcct tatctctgga taaggattca ttcattgcgt ttaaagatgc
     5161 ctgagccgct tttaatttct cattctgatt ctgaatatcc ttatcaagat agcgaacctg
     5221 atcctgccac ccggagccat attcatgagg cgggttataa gtcacaaaat catctttatt
     5281 tgcctctgcc tcttttacct ttttatcggc ttcctgaaca gcagcccttg caccggggag
     5341 cgccttctct gtcgatgcaa tgcggctgtt taaggtcgca atatttttat cttcggcatc
     5401 cagctcagct ttcgctttat catagtctct tttcagcccc tcttccggat gagcggcgtc
     5461 ccatgcctgc tggcgacgct tttcttcctc ctgacgctgt tttacctgtg ccggggtaag
     5521 aacatctgtc accgacacat aaaccggctt ctgtccggtc tctttcggga aacgaataac
     5581 ggcctcacgg gagttaccac cggctgtaaa accagccgga cgtgaatcac ctttttcagc
     5641 aacaatgcct tttggcgggg ctttcgctgt cggaacacct ttcggtacgc tcacctgcag
     5701 agacgggaga cccggaatcg acacactgaa tacccccgga cgttttgtcg gtttcgcatc
     5761 caccacaggg acactcattg gccgacctgc gacaaccgca atatgctgcc gctcatcctt
     5821 caccacatcc acaacccgtt gtctgacacg aaccgtcgcc tggtccagtg gtaaagtact
     5881 tgccggagtc tccgtcaccg catcggccgg taatgacgtc ataatttttg acatcatttt
     5941 cggatcatct tttgcaatct cagaaggcag cacaccgtag atcgcaatcc cccacagacc
     6001 aaacttaaac ggccccttca gggcagccag cacatcagca acggcggccg acaacgcatc
     6061 gccggaaact gataaagcca gtccttcagc ccccggagta gccagggcag gcagaccgaa
     6121 ggccatcgca gtggccgaag actgtccgcc accagagcta ccgctatgat tgccgctgtt
     6181 accaccatta ccaccgggtt tactcccgcc gttaccaaac tcccccggat tatagttatg
     6241 aatatctcca ttcggcgtat gcgtcgtacc ccagcccggc ccgctgtttg gatccgtacc
     6301 accacctgat gaagggccac ctttaccaga cgtaccgttc acattcccac tggcctgacc
     6361 accattatgt cccagacctg aattacccgg acctcgaccg tctccaccac tcataccgtt
     6421 ttcctctttg acatttaaaa caataaatta aagccaacat actgtacata tacacagtat
     6481 aattattacc ttactcctcc cgttttcagg tgtcaatata tgagattagt tctcatcttc
     6541 aggcacaaaa aagcgcccgg agccacctgt aagcagatcg cgtcttttcc cgaaaatttc
     6601 tcatggcaac aaattttatt atttaagaca ttcatcacac ttacatttca gatggtatag
     6661 acacatctga aagctaactg gcatgaggag aggacctgcg gttaaactgt tcgggaaacc
     6721 tgagacgccc ggaggccact atgacgcacc tgaaactgac tcccgctgaa gaaaagcgcc
     6781 tggcggacgc ctttgtcgct caggcactgg ccatgggcct gaaagggaac aaaacgctgc
     6841 caaaggcggt aaaacaacag gtgcgggaaa gggtgcttcg tgccattcag gacgaacgtg
     6901 aagccaggcg ggcgcaggaa atgcgtcggg agaaatacga ggctacggaa accaccttca
     6961 cctggcagcc acagcgccgc cggtaagggg tatttaccat aacgtcgcat tgcccgcacc
     7021 gctcagacgg tctgagggcg tttccggcgg ctacgggata aaacaggcaa caaaccaccc
     7081 ggaaaaccgc cggaaacgcg cccggtaaat tgtaacccgc tgcatgccta tgcacgcaac
     7141 tgcatggcgg gccgtttggt gtaaaaacga tacgataagc cgtgtttatg taaatgaacg
     7201 tggccgtaaa gagtgacaaa tatacatgta acatttttta ctgacaggtc atcaacccga
     7261 tgcgtttaat accctttatc ctgctggcat tgtgcagccc cttactcctg acggcctgta
     7321 agcattccac cacccagccg ccgaaaattt atactctgga taagtcatct aaaatcagta
     7381 gctacgctgc ggccatcaag accgctgttg aaatgcagat gtttgaacca gaggcctatg
     7441 cgggaaaaat gtgcaccgtg cggatatcca tacaacgtga tggcactctg aacagcgcca
     7501 ttgctgaagg aggagatccg aaactctgta aagcagcaat atcagccgtt acccacgcca
     7561 aaattccgcc ggcaccggat gaaaatacct ggcaaatatt caaaaacgcc cctctggatt
     7621 tcagaccatg aaagctttta ctctgacagt gctggcggcc atgttcctca ctccggtaca
     7681 tgcgatacca ctaaaacccg taaccacact gcagccaacc tacccggtac aggccgcagc
     7741 aaaacgcatc gaaggtcagg tgaccgccac gtttgatatt ctggacgatg gacgagtgga
     7801 caacgtgtcg cttaccggct gtcacctttt ttttcgggaa acgctgacgg caatgaggca
     7861 ctggctatat cccgcagggc acccggcaat gcgacaggtt ataaattttg aattcagtct
     7921 gaaaaatgac ggcttacagg atccgcgacc agtcaccacc caaaagctca atgatgatgg
     7981 ttgccgccag taacaggtcc tttaacccgc aggctgcaat agtcctggat gatgagtgag
     8041 cctgtagcag ccctgattag tggaagcgaa gctgaaaaat gggcagcaaa agccgtattt
     8101 ctggcaaata gcgccgccac ggattaaagt agtcatgtgt aatacatatg actacaaagg
     8161 ggcttttatg tcatcagcaa catccatcag gctcgatgaa gaacttaagg accggctgaa
     8221 aacgctggcc gatgaccggc accgctcagc gcacgcgcta atgctggagg caatcaccga
     8281 atacatcgac cgggaagaga agcgcagcca gtacctgcgg gacggacagg ccgcatggca
     8341 gcactatcag gaaacgggtc tgcaccttac tgcggaagaa gcggaagcct ggataagtac
     8401 ctggggaact gagaacgagc aggatgctcc gccatgccac aggtaacgat ttccgcgctg
     8461 gcacagcgcg atttacaacg ccttcaggac tttctcaaaa ccaaaaatcg gctggcagcc
     8521 agaaaaggag gtgaggtgat cgtccgggct atccagcaac tgaaaacatt gccagacatt
     8581 ggccgcccgg tgccgtttct gccgctggaa tataaggaac tggtgatcgg gtttggcgac
     8641 agtggctatg tgatgctcta ccgccacgac cgggaaatgg accagattgt gatcgtcacc
     8701 gtcaggcatc agaaagaatc cgggtatccg ggggcagaca gcctctaata accgatgacg
     8761 ggggcgcaat tcattgcgtg gccgtcgccg gtttgacaag cgcagcgcgt cagaaaacca
     8821 catcctgaac ggaaaaacgg gtaaaattca gcgatacatt taccgtgaga aattacacac
     8881 ctttcacacc cgataaaatc aggggtatac agtggcgaaa acattgctca acctttcaca
     8941 ggcagcacaa gccgcgggca ttaccagaag aacgctatac aatcatgtaa aacagggaaa
     9001 ggttaccgtg tcacgtgacg ggaaaaacaa ccctgtcgtg gatgtgtcag aactgatccg
     9061 tgtatacgga aatgtgaata tccctgagaa acaaattccc ggcatttcac accgtgaaaa
     9121 tacacaaaaa aatttcccac aggaacagct tctggcaatg caaaaggaac tggccgatct
     9181 ccggcaggct gtcaccctga tgctggaaga taaaacatcc cgggaagaag aacgccgcca
     9241 gcacgatgat gagcgccgga aactccaggc cgaagtggac agattgacaa cagaactgac
     9301 acagaagaaa aaacgcttct ggtccggctg gtttagctga gaacttaccg aagggaatgg
     9361 agaacatgac gaagcaggaa aaaactgccc tcaatatggc taaattcatt aaggatcagt
     9421 cgctgctgtt gctggaaaag ctgaacgagc tggacatgga taccccggcc gacatgtgcg
     9481 aacggcttca cgaggatgcg gaaacactct ggcaggtgat gcgggataag ctcggggaag
     9541 aataacagca ccggccatgc cggtgctggc gttatttttt gccgaacggc caccaccggc
     9601 gggcgggttt gtcctccggc agttcaggca ccggcacgtt aaaatagcgg ctgttatatt
     9661 cccgcatcag ctccgctttc agcttctcgt tcaccgcgct ttccccgtgc cgctcgctga
     9721 ccacctcaat catcgcgtta atctgctccg gtgagcactt cccggcatca accacctgca
     9781 caccgttctc gatggcgaac cgctccacct ttcgatagag gtaatcacac agcttcattc
     9841 ctgtgggttt gtcccgttcc cattgcggat tttcaccggt gaaaaagtac gtggcttttt
     9901 cgtcgctgtt gagccgctcg tcatacatcc cgatggattt catcatcgcc agatgctggg
     9961 cccggtcgac aaaacagaaa acatacgccc cggtctgcca ggcgagcacc accatgtctt
    10021 ccagatgctc gctggttggc ataatgcagc gctcgccgtc
//
