LOCUS       NZ_CP147168             5596 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain SC41 plasmid p3, complete sequence.
ACCESSION   NZ_CP147168
VERSION     NZ_CP147168.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40144598
            Assembly: GCF_037157725.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Wang,D.
  TITLE     Klebsiella pneumoniae strains whole genome sequences
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Wang,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2024) School of Life Sciences, Taizhou University,
            1139 Shifudadao Avenue, Taizhou, Zhejiang 318000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.15.3; Canu v. 2.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina NovaSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037157725.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 18:35:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,626
            CDSs (total)                      :: 5,503
            Genes (coding)                    :: 5,372
            CDSs (with protein)               :: 5,372
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 48 of 131
            Pseudo Genes (incomplete)         :: 83 of 131
            Pseudo Genes (internal stop)      :: 16 of 131
            Pseudo Genes (multiple problems)  :: 16 of 131
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147168.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SC41"
                     /isolation_source="bronchoalveolar lavage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p3"
                     /geo_loc_name="China: Taizhou"
                     /lat_lon="28.40 N 121.26 E"
                     /collection_date="2021-08-19"
                     /collected_by="Dongguo Wang"
     gene            join(5270..5596,1..219)
                     /locus_tag="WBS30_RS28080"
     CDS             join(5270..5596,1..219)
                     /locus_tag="WBS30_RS28080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            285..635
                     /locus_tag="WBS30_RS28085"
     CDS             285..635
                     /locus_tag="WBS30_RS28085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(705..971)
                     /locus_tag="WBS30_RS28090"
     CDS             complement(705..971)
                     /locus_tag="WBS30_RS28090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(955..1179)
                     /locus_tag="WBS30_RS28095"
     CDS             complement(955..1179)
                     /locus_tag="WBS30_RS28095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(1846..2058)
                     /locus_tag="WBS30_RS28100"
     CDS             complement(1846..2058)
                     /locus_tag="WBS30_RS28100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2065..2574)
                     /locus_tag="WBS30_RS28105"
     CDS             complement(2065..2574)
                     /locus_tag="WBS30_RS28105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2609..3313)
                     /locus_tag="WBS30_RS28110"
                     /pseudo=""
     CDS             complement(<2609..3313)
                     /locus_tag="WBS30_RS28110"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3252..3596)
                     /locus_tag="WBS30_RS28115"
     CDS             complement(3252..3596)
                     /locus_tag="WBS30_RS28115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3586..3762)
                     /locus_tag="WBS30_RS28120"
     CDS             complement(3586..3762)
                     /locus_tag="WBS30_RS28120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4402..4560
                     /locus_tag="WBS30_RS28125"
     CDS             4402..4560
                     /locus_tag="WBS30_RS28125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4918..5244
                     /locus_tag="WBS30_RS28130"
     CDS             4918..5244
                     /locus_tag="WBS30_RS28130"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     Typing          complement(3849..3925)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          4114..4511
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(1731..3262)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagaaaaatg ataatccact aatatttata atgagcattg aagaaagtat aaaagaaacc
       61 cttaactcag gttatatgtt atatagagta gctcccataa ctgagggtga agcagctgct
      121 ttcatggatg ttcttaaaca attattatta tttgaggata attttcgcct ttttggtaga
      181 gacttggttt ataaaataga aaaattaaaa atcagataac aaagtatggt gaacgaaaaa
      241 tgaaagagag gatagtgaaa tgtgtaatac caagattctc aactatgtat tggttaattg
      301 cgttcctagc atttatattc cccggtcgat cttttgctga cggttacacc gcttactacc
      361 gatgtaatgg tactcaggaa gcatctctta ctgttaaaga taacaaacta agtatggttg
      421 taggtctttc taagtttaat aggcaactgg atacttatag attcaaagaa aagggaatcg
      481 aagtagtaat gcagagagct gcttctgatg ataactctga ggccataatg tcttttatta
      541 tgcctgacgt taacaatatg accttattag ttaaaaataa cggctacatt atcgttaaga
      601 caacctgctg gttaactaaa accgacaaac agtagaacac gtagttagtc gagtatttac
      661 cataatacct gttgtcggca tcaaaaaagc ccgcatagcg ggcttcagtc gtaaacttcg
      721 cggcgatgtc caatcgtggc ggcaaggata acaagctcgt catctcggat ctcacacaga
      781 acgcgataat ctcccacccg atagcgccag aactcgccca gctcaccttt gagcggcttc
      841 cctgactggc gaggatcggc agcaactgca atgcgtaaac tcataaaatc cacaatccgt
      901 cgtgcgttct gtttgtccat cttgcgtaac gatttgagcg cccgatcgga atagttaatc
      961 gtccagacca agtgacttcc tcacgtcttc agacgaatgc actgcttcat caccacggcg
     1021 aacgcgtgca gcagtttctg ctgaaagata ataatcctcc aggtcttcca gatgcgccag
     1081 tattgcttcc cgcgcataaa acgtctttgt tctgccggtt tgcttcgcca gcgagtccag
     1141 acgggactca atctcatcag ataaccggat agcaagcata taaacctccc gaacaccatt
     1201 tgctatacat gtatatcaac agtaccacaa tcaacctaaa agaacaaaaa ataatcttgt
     1261 tagcccctct tgataatccg taacgcggtc aatatatcta gcttgagggc tttcctctta
     1321 actattgaaa ggataacgat tatgggtctt aagccgggtc cgaaacgaat tgccgattca
     1381 acaggggaac ctgataaacg ccagcgtgac aataaaaaaa cgccgggaaa tactgacaaa
     1441 ttgaaaccca gcaaatcttc aaaaaaataa gtcagtagtt aaaagccctc cggggctttt
     1501 ttgtatctgc cgttcacgct gattaaacat aacacccgtt gcccgcaccg ccccggacgg
     1561 gttcgccgcg aacggggtgg cagtcaggta aaaccggcaa caaaccaccc gataaaccgc
     1621 cccgttcgcg acagataaat tttacctggc tgcatagcca tgcagccagg tgaatgacat
     1681 ccggtttcag cgtaaatgcc cgatgagaac cctgttttcc tgcatttcag cccagcgacg
     1741 gaccgtcata gcttcgctgc cgggaacgcg cttccgtcag ttccttttcg tgctgccgtt
     1801 gctcgtgcgc ccgttctgcg gccacggcca tctcatgacg ctgcactacc ggctcaaacg
     1861 ctgcagttgc ccgctcaagc gttccacctg ctgactcaag cgcgtcacgc gctcgctcag
     1921 cgccgcgttc tcccgttgca taagccctga cattttccgc cattccccga aggcgctctc
     1981 ccactcgtcc agccttttcg agtagtcctg ctgtagctgc tctaatgcgc tcagcaactg
     2041 tttttccagc tcggtcatcg gcacttactc cttcaccagt ctcagcgcgt tctgcttcga
     2101 ccgcccgttc ccgaccgtcc agctctgcgt cctgtctacg gccgtcccct tcggcagcac
     2161 cagaaaacgt ccgttgctgt cttccaggta cgtcacgccc caggtcttcg cgttcagctt
     2221 cgacagcgct gcgttctgcc ggtcgatttc ggccagattt gacgctatca agctcccctg
     2281 ataccacaac accccgctca ggctggcgaa cagcagtcct gacaccatcg tgatggtcag
     2341 ccatgtccgc ccgaccatac gcatcacgct caggcggttc gactgcatgg ccgcgctcat
     2401 accctgctcg tgggcgcgga tggcggcgct gattctcttc ccgctttcgc tcagttccgc
     2461 tctgacagac ttctcgtgct cgctgaatgc ggctttcagc atttcgccgg tactctgctg
     2521 ctgcgctttc gatttctgct ctaagtcctt cgccagcgtt aaaagactgt tcatagatgg
     2581 ctcccttaag tcggatgttt cgccctccgt ccgggtcggc aatgctgatg ctgctctttg
     2641 tggtgcgcac gacctcaaaa cctgcgcttt ccagcgcctc agtgacgtcc tgacgcgttt
     2701 taagctcccc tgacgaggcg agggccagta agccccgcgt aatagcctct gcggcttcct
     2761 gcttcgtttt gggtaacgaa gatggcgtca ccagcgcgcg ccggttctcc ggtgcgttcg
     2821 ggtcgtgcag ccccagtcgc ccgttcacca cggtctgcca cgcatcgatg cgcggacggt
     2881 ctgcccggtc gtagtacggc tggagacgcc tgccggtcag cagttccgtg ttcggtacca
     2941 gaaaattcag ttccagccgc cccttgtccc ggtgttcaac ccacagcacg ctgtactggt
     3001 ctttatcgag tccgggcatc agaacccgct cgaagctcgc catcagcttt tcacgctggc
     3061 cgggcggtaa atcctgttca gcaaaagaca ggacgccgga ggtgtacttt ttggcgtagg
     3121 gcgaggcgtc gataagctcc cggacctcct ccggctttcc ctgcaggacg atggccccgt
     3181 cgcgctggcg gtctttcccc agcagataat ccaccggccc ggcaccgccg cctcgccctc
     3241 gcggatgaaa cttaacgatc atcgtctgcc cccttttcca gcacggcatg ccgcagccgc
     3301 tcgagttcgg catcaatggc catcagcgcg gccaccacct gaacccggtc agcgcctgac
     3361 cactggccgc cgttaatttt ccgggcgatc tggttgaggt tgttccccat cccggcaagc
     3421 tggcgcagca aaaccggatc gatggacggc agccgcaacg accgcgccgg gcgcgtatcg
     3481 agacaggttt cccgcatcca caccgccagt tgccttccat tgcagcgctc cagcagctgc
     3541 tggtactccc catcggtcac ccggatacct aaaaaacggg tacgcttaac cgccatcctc
     3601 tcctccgata tccgcaaaat gtgaccgggc tgaacgctca agctctgcca tctgctcccg
     3661 gcggtaatct tcctcctgtg cctgacgcat cagatttgcc atcactgccg gatgccagga
     3721 tctttttttt acgacgagcg gcttcttctt cggcttctgc attatccctc cttcgcaaac
     3781 gccggacggc acggaaaagt tttccgggtc gctccggcgt tggacaagcg ccagcgcgtc
     3841 agtggtgtta gcgggtttcg gggcgcagcc ctgaaccagt cacagcgttt acgctgtgta
     3901 tactggctta ctatgttggc atgagagcac gattcatgaa agtgcctcat gtggcaggtg
     3961 tgatatgtgg ctcaggagcg tccgcaatac catgcacgga cagatgaaat gacgcctcct
     4021 cgctcactcg gtagctgcgc tcctgccgtt cggctgcggc gggcgtgacc gactcacgga
     4081 taacgggata aagcaaaggc gctcaaatca gaaagggccg gagaaaagga tgcggcgttg
     4141 ccgtttttcc ataggctccg cctcccctga cgagcatcac agaaatggac gctcaagtca
     4201 gaggaggacg aaacccgaca ggacttaaag atgccggtga tttctctccg gcggctccct
     4261 cgtgcgctct cctgttccga ccctgccgtt taccggatac ctgttccgcc tttttccctt
     4321 tcgggaagcg tggcgctttc tcatagctca cgctgctggt atctcggctc ggtttaggtc
     4381 gttcgctcca agctgggctg tatgcacgaa ccccccgttc agcccgactg ctgcgccctt
     4441 tccggtaact atcatcttga gtccaacccg gacggacacg caaaaacgcc actggcagca
     4501 gccattggta actggatata tacgagagag atattgagag gtcttgaagt gggtgcctaa
     4561 ctgcggctac actgaaagaa cagttttggt atctgcggtc tcgtataccc agttacccgg
     4621 ttaagcagtt ccccaactga cttaaccttc gatcaaaccg cctccccagg cggttttttc
     4681 gtttacagtg caagagatta cgcgcagaaa aaaaggatct caagaagatc ctttaaatga
     4741 tcgtcagttc tgttatcccc ggagctccat ccatcccacg gaaaaacacc cgccactgat
     4801 gccctcagcg cactgcacat catgtacaaa ccactccctc cctccttttt tattgcgttt
     4861 cttaatgcgc aatggtagct ttgtgcgcaa caaaaaacgc aatagcaagg gggggtaatg
     4921 agagaaagaa caaatatcat gctagatata ggattaaagc aatccttaca acggctttcg
     4981 aataaaacca aaatgagtct aagtgatctt attaaccaga gattatcagg ttcactttca
     5041 gttgaagaac gcattggcga aaagcctgaa tggatgagaa accttgatcc ctatctcgcc
     5101 aatcactttt caaatattga gttaccgctc ccttcaccat taaaagaata ccaagaaaaa
     5161 atggatgaga tagattcaat tattaatgac atgatggttc taaaggaata tttcagagct
     5221 aagttttacg acgaaataaa ataggcacgc tatacggagg cggagaatta tgttttttaa
     5281 acggtattta acggagaagg aaatatacaa ggcagcactc ccaattgtta ggcttcaagg
     5341 gagagttaac acttcattac gttttttatc caaatactca gatgtaaaca aaaatggatt
     5401 aattgcattg catcttaatt tattacattt tatctcatac aacatacacg gtgtagagtc
     5461 tctgaaaatc actcaagcag gcataaacaa agctgtacaa caaagccctc acgcagatga
     5521 aataaaaaat attgcgcagg caataggacg caaaggacct gagtacatcg aaggattaat
     5581 tagaaaccca tggggg
//
