LOCUS       NZ_CP147167            11969 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain SC41 plasmid p2, complete sequence.
ACCESSION   NZ_CP147167
VERSION     NZ_CP147167.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40144598
            Assembly: GCF_037157725.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11969)
  AUTHORS   Wang,D.
  TITLE     Klebsiella pneumoniae strains whole genome sequences
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11969)
  AUTHORS   Wang,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2024) School of Life Sciences, Taizhou University,
            1139 Shifudadao Avenue, Taizhou, Zhejiang 318000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.15.3; Canu v. 2.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina NovaSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037157725.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 18:35:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,626
            CDSs (total)                      :: 5,503
            Genes (coding)                    :: 5,372
            CDSs (with protein)               :: 5,372
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 48 of 131
            Pseudo Genes (incomplete)         :: 83 of 131
            Pseudo Genes (internal stop)      :: 16 of 131
            Pseudo Genes (multiple problems)  :: 16 of 131
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147167.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11969
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SC41"
                     /isolation_source="bronchoalveolar lavage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2"
                     /geo_loc_name="China: Taizhou"
                     /lat_lon="28.40 N 121.26 E"
                     /collection_date="2021-08-19"
                     /collected_by="Dongguo Wang"
     gene            814..2778
                     /locus_tag="WBS30_RS28010"
     CDS             814..2778
                     /locus_tag="WBS30_RS28010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            2778..3509
                     /gene="mobC"
                     /locus_tag="WBS30_RS28015"
     CDS             2778..3509
                     /gene="mobC"
                     /locus_tag="WBS30_RS28015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            3516..4046
                     /locus_tag="WBS30_RS28020"
     CDS             3516..4046
                     /locus_tag="WBS30_RS28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4073..4252)
                     /locus_tag="WBS30_RS28025"
     CDS             complement(4073..4252)
                     /locus_tag="WBS30_RS28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4278..4706)
                     /locus_tag="WBS30_RS28030"
     CDS             complement(4278..4706)
                     /locus_tag="WBS30_RS28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(4870..5184)
                     /locus_tag="WBS30_RS28035"
     CDS             complement(4870..5184)
                     /locus_tag="WBS30_RS28035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5172..5450)
                     /locus_tag="WBS30_RS28040"
     CDS             complement(5172..5450)
                     /locus_tag="WBS30_RS28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(5625..5990)
                     /locus_tag="WBS30_RS28045"
     CDS             complement(5625..5990)
                     /locus_tag="WBS30_RS28045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(5987..6358)
                     /gene="tolA"
                     /locus_tag="WBS30_RS28050"
     CDS             complement(5987..6358)
                     /gene="tolA"
                     /locus_tag="WBS30_RS28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(6632..6877)
                     /locus_tag="WBS30_RS28055"
     CDS             complement(6632..6877)
                     /locus_tag="WBS30_RS28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            7522..9009
                     /gene="ltrA"
                     /locus_tag="WBS30_RS28060"
     CDS             7522..9009
                     /gene="ltrA"
                     /locus_tag="WBS30_RS28060"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            9114..10799
                     /locus_tag="WBS30_RS28065"
     CDS             9114..10799
                     /locus_tag="WBS30_RS28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            10809..11066
                     /locus_tag="WBS30_RS28070"
     CDS             10809..11066
                     /locus_tag="WBS30_RS28070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            11151..11300
                     /locus_tag="WBS30_RS28075"
     CDS             11151..11300
                     /locus_tag="WBS30_RS28075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             4114..11969
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(11404..11968)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.243"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          2779..3506
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 gttttgcctc acatgttagt cccctgctta tccacggaat ctgtgggtaa ctttgtatgt
       61 gtccgcagcg cccgccgcag tctcacgccc ggagcgtagc gaccgagtga gcgaggaggc
      121 gcaatttccc cggcacctgc aagacggtca ccggacgcgc tgatgtggga tattgtcccc
      181 tgattaagtc tttctattcc ctgacgatca attatcagga taggtgaacc atgtcggttt
      241 ttcgctgaac attttacgtt aacctatctc tgttgctttt ttctccttgc ggagaaatgc
      301 aagtcatccc cgctggggcg tggctgactt caggggaaaa gaccttgctt acgctggctg
      361 gacgccaccg acgcacggtc ttttcccctc tttctggctc tgtccggctg gacgccggcc
      421 acgccagaaa ttcacccggc ccccgtcagg ctcacgcctg accacccaca aaaatccacg
      481 gtgctggacg cacctgacga agcagcaaag ctgctcctga ttttgcgggt ggtcagccgc
      541 tcgctatggc gaactctgac gggggccgga acaccggaaa agaccgcact ggggtgccgc
      601 cttttcctct cacacgctgg ggcgtgctcg ttgttcctcc ggggctgcgc cttttccgca
      661 tggggcggaa agaggctggc ctccgcggtg aaagctgaac gcaatcacca cggacgtgac
      721 cgccgccgcc atgccttggg gcatgtccgc ggcttgtgca gcgggggctg cgcatcacgc
      781 cggtaaaccc gtcgtctgaa ggagagagat tgcatgttta atacggattc gcttgcctgg
      841 cagtggcata tcccggagct ggcccggcag atttacccgt gggtcagctg gctgtaccgg
      901 gagtattaca gtctggccgg tttttttctg gttatcggcc tgtttttcgg tctggctgaa
      961 ctctgggccg gccgaatgcg ttttgtgggg ctgagccata aggcgcggcg ggagtatgcc
     1021 gatcgctata agcgcgataa taaccggact gtgccatttt cggtgctggc ctcgctggat
     1081 tcacccgggc ggatggtcag ggcacggcga catgtctgga actacgcgta ctggctgacc
     1141 ggcgctgccg gtctggccct tctggggggg ctggttgtcg ccgtgtttat cgagagctgg
     1201 ggagaaaccg tcctgcgccc ggacctctat gcgaacgcag attattacct gctgcagccg
     1261 gctaacctgt tcgggatagc ctggtgtgcc ggagggcttg tcgccggcgt ccttctggcg
     1321 cggctgctgg cgttcctgct gcttgatggt cattttgcgg ccgctgacga ggcggtgaac
     1381 gcgaaactga atcaggagag ccggcgcagc agccagcgga ccggagaaat gacagacgtc
     1441 cgccacctgc atttcgggga gccggtaccg gttaacgcgc tggccgattt ttccacggag
     1501 caggcccgga aacagcaggc ggtgtttctg ggtaaggatg agcagggcca gccggtgctg
     1561 gtccctcgtg atacctggcg caagaccaat attcagatcc tcggcctgcc cgggagcggc
     1621 aagagcgcga tggccaccaa cgcgctcatt cgctgcgtac gcgatttcgg ggacgctgtg
     1681 gtgtacttcg accccaaagg ggacgcgtgg gcgccgcacg tcttccgggc gcactgcccg
     1741 gacttcaccc tgctggatct gcggccgggg aaaccggcgc agctgaatct gttccgcgat
     1801 ctcgaccagt acgccctgaa gaatctgctg gttgccgggt ttaacctgag tgagaccggc
     1861 gacgtggcgg accactaccg catcagcgag cagaaagcgg cgaagctgat tgcggagcag
     1921 ttcccgcagg gggcaaacat tcagcaggtg ctggcggcgg cgtacgcgtt gccggaggcc
     1981 ctgaaaaagg acgtgaaggg gctgatcacc aagctggaga acgtggccga cctgagcgtc
     2041 ctgcagacgg acagcggtat cgatgtggcg gggataatca acggcggcgg ctgcctgtac
     2101 gtcatcggct cgatggacga tgaggcggtg atccgggtgc agaagatgct gtttgcccgc
     2161 tgcgcgcaaa tcatcattgc gcgggatgag ttccggaggt ggccgcacgc gagcatcatg
     2221 ctcgatgaga ttaaatatct gctgtcgaag tacgtgctga acgcgctggg gacgctgcgc
     2281 agccgggact gcaacctgct gctcgcgcac cagtcgctgg gcgacttcgg tcagtgcggg
     2341 caggatctgc ccgctgattt tgtgaaaacc acggtactgg ataacacgcc gatccgctgg
     2401 ttctatcgcg cggccagcca ggagtccgcg cagtgggcgg cgggccagac cggggagatc
     2461 cgggtggacg tggagcgccg ccgggccagc cgggaggcgg ggaacgtgga gcacatcagc
     2521 ggcgacagct tcatccagaa ggaggcgcgg ccgctgtttg acgtgaacac cctgcaacac
     2581 ctgcctgacg ggttcgcggt gatgaccggg ctgggcgtgg cgcggctggc gttcagcagc
     2641 ccgctgcggg ttgagcgccg ggaaatttcg ctgaaaacct tcccggtgct ggcgaaaacg
     2701 gacccgctgg cggagtacct gcctccggcc gacggcggcg gaccaaaagc ggatgatgat
     2761 tttgcggggc tttactgatg acactggagc agtacaacgt cagccacgcg aagcggcagg
     2821 cgcggaacgc ggagaaaaca cggctgaccc tgcgctggct gcgggaagag ctgtgttcca
     2881 cggcggagct ggttgcgcga cggctgggga ttgcggcggt acagccggtt taccgcttcc
     2941 tggacagtct ggtggcgaaa gggctgctgg tccgggcgaa atacccggtt gacggccgtc
     3001 aggtcagcgt gtgggggctg acgccgcacg gggtggcgtt ctcgttcgac gaggacgagc
     3061 cgctgacgga cgttattccg tttcagccgt caagggtgtc ggcggcgcag ctgccgcacc
     3121 ggctggccgt gcagtccctg cgcctctcca tggagggccg gggggccacc ggctggcggt
     3181 acctgcaccg tatggccctg aaagggatga aagtgccgga cgcgctggcg gagctggacg
     3241 gcagaacggt ggcatttgag gtagagcgca ccgtcaagag ccgcaggcgg tatcaggaag
     3301 tggtgtccgg gtatctgttc aaccgccggg cgaacggcat cgatgaaatc tggtacatct
     3361 gccctgaccg gccgacgctt gtccgggtac agcgggcgat tctggcggtg gatgaaatcg
     3421 tgaatccaca gaccggcgag gcccgcaaaa cggtggagct ggaccgggag aggctgttcg
     3481 cgtgctttaa atttatgaca acggagtgag ggattatggc gctgtacatg gtatttattg
     3541 aggacgtgga agacaaagac ggcgagcgct gcattatgcc aaccagcgag catctggaag
     3601 acatggtggt gctcgcctgg cagaccgggg cgtatgtttt ctgttttgtc gaccgggccc
     3661 agcatctggc gatgatgaaa tccatcggga tgtatgacga gcggctcaac agcgacgaaa
     3721 aagccacgta ctttttcacc ggtgaaaatc cgcaatggga acgggacaaa cccacaggaa
     3781 tgaagctgtg tgattacctc tatcgaaagg tggagcggtt cgccatcgag aacggtgtgc
     3841 aggtggttga tgccgggaag tgctcaccgg agcagattaa cgcgatgatt gaggtggtca
     3901 gcgagcggca cggggaaagc gcggtgaacg agaagctgaa agcggagctg atgcgggaat
     3961 ataacagccg ctattttaac gtgccggtgc ctgaactgcc ggaggacaaa cccgcccgcc
     4021 ggtggtggcc gttcggcaaa aaataacgcc agcaccggca tggccggtgc tgttattctt
     4081 ccccgagctt atcccgcatc acctgccaga gtgtttccgc atcctcgtga agccgttcgc
     4141 acatgtcggc cggggtatcc atgtccagct cgttcagctt ttccagcaac agcagcgact
     4201 gatccttaat gaatttagcc atattgaggg cagttttttc ctgcttcgtc atgttctcca
     4261 ttcccttcgg taagttctca gctaaaccag ccggaccaga agcgtttttt cttctgtgtc
     4321 agttctgttg tcaatctgtc cacttcggcc tggagtttcc ggcgctcatc atcgtgctgg
     4381 cggcgttctt cttcccggga tgttttatct tccagcatca gggtgacagc ctgccggaga
     4441 tcggccagtt ccttttgcat tgccagaagc tgttcctgtg ggaaattttt ttgtgtattt
     4501 tcacggtgtg aaatgccggg aatttgtttc tcagggatat tcacatttcc gtatacacgg
     4561 atcagttctg acacatccac gacagggttg tttttcccgt cacgtgacac ggtaaccttt
     4621 ccctgtttta catgattgta tagcgttctt ctggtaatgc ccgcggcttg tgctgcctgt
     4681 gaaaggttga gcaatgtttt cgccactgta tacccctgat tttatcgggt gtgaaaggtg
     4741 tgtaatttct cacggtaaat gtatcgctga attttacccg tttttccgtt caggatgtgg
     4801 ttttctgacg cgctgcgctt gtcaaaccgg cgacggccac gcaatgaatt gcgcccccgt
     4861 catcggttat tagaggctgt ctgcccccgg atacccggat tctttctgat gcctgacggt
     4921 gacgatcaca atctggtcca tttcccggtc gtggcggtag agcatcacat agccactgtc
     4981 gccaaacccg atcaccagtt ccttatattc cagcggcaga aacggcaccg ggcggccaat
     5041 gtctggcaat gttttcagtt gctggatagc ccggacgatc acctcacctc cttttctggc
     5101 tgccagccga tttttggttt tgagaaagtc ctgaaggcgt tgtaaatcgc gctgtgccag
     5161 cgcggaaatc gttacctgtg gcatggcgga gcatcctgct cgttctcagt tccccaggta
     5221 cttatccagg cttccgcttc ttccgcagta aggtgcagac ccgtttcctg atagtgctgc
     5281 catgcggcct gtccgtcccg caggtactgg ctgcgcttct cttcccggtc gatgtattcg
     5341 gtgattgcct ccagcattag cgcgtgcgct gagcggtgcc ggtcatcggc cagcgttttc
     5401 agccggtcct taagttcttc atcgagcctg atggatgttg ctgatgacat aaaagcccct
     5461 ttgtagtcat atgtattaca catgactact ttaatccgtg gcggcgctat ttgccagaaa
     5521 tacggctttt gctgcccatt tttcagcttc gcttccacta atcagggctg ctacaggctc
     5581 actcatcatc caggactatt gcagcctgcg ggttaaagga cctgttactg gcggcaacca
     5641 tcatcattga gcttttgggt ggtgactggt cgcggatcct gtaagccgtc atttttcaga
     5701 ctgaattcaa aatttataac ctgtcgcatt gccgggtgcc ctgcgggata tagccagtgc
     5761 ctcattgccg tcagcgtttc ccgaaaaaaa aggtgacagc cggtaagcga cacgttgtcc
     5821 actcgtccat cgtccagaat atcaaacgtg gcggtcacct gaccttcgat gcgttttgct
     5881 gcggcctgta ccgggtaggt tggctgcagt gtggttacgg gttttagtgg tatcgcatgt
     5941 accggagtga ggaacatggc cgccagcact gtcagagtaa aagctttcat ggtctgaaat
     6001 ccagaggggc gtttttgaat atttgccagg tattttcatc cggtgccggc ggaattttgg
     6061 cgtgggtaac ggctgatatt gctgctttac agagtttcgg atctcctcct tcagcaatgg
     6121 cgctgttcag agtgccatca cgttgtatgg atatccgcac ggtgcacatt tttcccgcat
     6181 aggcctctgg ttcaaacatc tgcatttcaa cagcggtctt gatggccgca gcgtagctac
     6241 tgattttaga tgacttatcc agagtataaa ttttcggcgg ctgggtggtg gaatgcttac
     6301 aggccgtcag gagtaagggg ctgcacaatg ccagcaggat aaagggtatt aaacgcatcg
     6361 ggttgatgac ctgtcagtaa aaaatgttac atgtatattt gtcactcttt acggccacgt
     6421 tcatttacat aaacacggct tatcgtatcg tttttacacc aaacggcccg ccatgcagtt
     6481 gcgtgcatag gcatgcagcg ggttacaatt taccgggcgc gtttccggcg gttttccggg
     6541 tggtttgttg cctgttttat cccgtagccg ccggaaacgc cctcagaccg tctgagcggt
     6601 gcgggcaatg cgacgttatg gtaaataccc cttaccggcg gcgctgtggc tgccaggtga
     6661 aggtggtttc cgtagcctcg tatttctccc gacgcatttc ctgcgcccgc ctggcttcac
     6721 gttcgtcctg aatggcacga agcacccttt cccgcacctg ttgttttacc gcctttggca
     6781 gcgttttgtt ccctttcagg cccatggcca gtgcctgagc gacaaaggcg tccgccaggc
     6841 gcttttcttc agcgggagtc agtttcaggt gcgtcatagt ggcctccggg cgtctcaggt
     6901 ttcccgaaca gtttaaccgc aggtcctctc ctcatgccag ttagctttca gatgtgtcta
     6961 taccatctga aatgtaagtg tgatgaatgt cttaaataat aaaatttgtt gccatgagaa
     7021 attttcggga aaagacgcga tctgcttaca ggtggctccg ggcgcttttt tgtgcctgaa
     7081 gatgagaact aatctcatat attgacacct gaaaacggga ggagtaaggt aataattata
     7141 ctgtgtatat gtacagtgtg cgccgggaga ccggttagag atcaaggggt gaaagtcccc
     7201 gaccattgaa ggaccagcaa tccacaaggt ccccgagtca tgcgttgcat accgcgaggt
     7261 atggggcgaa gcgttgacag gggtgttgac aggccagcca ttgagccacg aaatgtatat
     7321 taaattaccg ggtgccgacg ttgtactgtt aacggaaggc aacatcatag ggtgcgatac
     7381 tgcgagtgcc acatggaccc ggcggggtct gagaccctgg catgtcaata cgatctctac
     7441 gcgggaaccg ggagatctcc cctctgacca tctgccagtg tcggagatgg cccgcaccgg
     7501 gaagacgagg agtcatagcc ggtgatgtac ggagaggaga agtcggactc gctcatagta
     7561 gcggcgaagc aggcgaacaa cccgaaagga gcggagtcag tggagcgaag gagcggggcc
     7621 aaggggaacg cggaacagcc acacatgcgc cggacacaga gccgggaaag catgtcacag
     7681 aggctgtcac gcgtgcggga agctgcgaag cagcggaaga aagaacggtt tacagcattg
     7741 ttccacctgc tgacagtcga agcactggaa gccgcattcc tctccctgag caggaaagcg
     7801 gccgccggag tggatggcat caggtggatg gactacgccg gaaacatgaa gaacaacata
     7861 acagatctgc accggaggct acatcagggc agctacaggg cgcagcccgg caggcgtcac
     7921 tacatcccaa aagcggatgg aaaacaacgc ccgctcggca tcgcctcgct ggaggacaag
     7981 atcgtccagt atgcgctggt gaaaatcctg aacgcagtct atgaaaacga ctttatgggg
     8041 ttctcatacg ggttcagacc cgggcgaagc cagcacgatg cactggacgc actggccaca
     8101 gggctggtac gcactaacgt aaactgggta ctggatgccg acatcagtca gttcttcgac
     8161 agggtgagcc acgaatggct gatcaggttc acagagcatc ggatcggcga ccggagggta
     8221 atcaggctca tacgtaagtg gctcacagcc gggacgtcgg aggagggtca atggcgagca
     8281 acggaggaag gcaccccaca gggtgcggtc atctcaccgc tgctggcaaa catatacctc
     8341 cactacgtct tcgatctgtg ggcgcatcag tggcgacgtc gctatgccac aggcaatgtg
     8401 gtaatggtca gatacgccga tgacatcgtc atcgggttcg acaaacgata cgatgcccgg
     8461 cgcttccgta tagccatgca gcgcagactg agggagttcg gactcacggt tcacccggag
     8521 aaaacccgtc tgatggagtt cggccgcttc gctgccgaaa accgtgccat caggggaaaa
     8581 ggcaaaccag aaacgttcaa cttcctcggg ttcacgcaca tcagcgggaa agatcgcaac
     8641 ggcaggttca tgctgatacg aaagacccgc cgggatcgga tgacggcaac tctgaaagcc
     8701 atcaaagacg gtctgcgaag gcgctggcat tactcaatcc ccgaacaggg aaaatggctc
     8761 aggagagtgg ttcagggata cctgaactat cactcggtac cgggcaactt ccccaccatg
     8821 cagaagttca ggacacacgt aacaaacctc tggcgccggg cgctcaggcg caggagccag
     8881 aaggatgata cgacctggac gaaagcaaac aaactggcag ccgcatggct accaagggtt
     8941 cgggttcttc atccatggcc tgtggagcgg ttcaccgcca gacacccgag gcaggagccc
     9001 ggtgcgtaaa tcgcgcacgc cgggatctgt gcggggggta tccggtaacg ggtatcccta
     9061 ccgcgacatg ttggctttaa tttattgttt taaatgtcaa agaggaaaac ggtatgagtg
     9121 gtggagacgg tcgaggtccg ggtaattcag gtctgggaca taatggtggt caggccagtg
     9181 ggaatgtgaa cggtacgtct ggtaaaggtg gcccttcatc aggtggtggt acggatccaa
     9241 acagcgggcc gggctggggt acgacgcata cgccgaatgg agatattcat aactataatc
     9301 cgggggagtt tggtaacggc gggagtaaac ccggtggtaa tggtggtaac agcggcaatc
     9361 atagcggtag ctctggtggc ggacagtctt cggccactgc gatggccttc ggtctgcctg
     9421 ccctggctac tccgggggct gaaggactgg ctttatcagt ttccggcgat gcgttgtcgg
     9481 ccgccgttgc tgatgtgctg gctgccctga aggggccgtt taagtttggt ctgtggggga
     9541 ttgcgatcta cggtgtgctg ccttctgaga ttgcaaaaga tgatccgaaa atgatgtcaa
     9601 aaattatgac gtcattaccg gccgatgcgg tgacggagac tccggcaagt actttaccac
     9661 tggaccaggc gacggttcgt gtcagacaac gggttgtgga tgtggtgaag gatgagcggc
     9721 agcatattgc ggttgtcgca ggtcggccaa tgagtgtccc tgtggtggat gcgaaaccga
     9781 caaaacgtcc gggggtattc agtgtgtcga ttccgggtct cccgtctctg caggtgagcg
     9841 taccgaaagg tgttccgaca gcgaaagccc cgccaaaagg cattgttgct gaaaaaggtg
     9901 attcacgtcc ggctggtttt acagccggtg gtaactcccg tgaggccgtt attcgtttcc
     9961 cgaaagagac cggacagaag ccggtttatg tgtcggtgac agatgttctt accccggcac
    10021 aggtaaaaca gcgtcaggag gaagaaaagc gtcgccagca ggcatgggac gccgctcatc
    10081 cggaagaggg gctgaaaaga gactatgata aagcgaaagc tgagctggat gccgaagata
    10141 aaaatattgc gaccttaaac agccgcattg catcgacaga gaaggcgctc cccggtgcaa
    10201 gggctgctgt tcaggaagcc gataaaaagg taaaagaggc agaggcaaat aaagatgatt
    10261 ttgtgactta taacccgcct catgaatatg gctccgggtg gcaggatcag gttcgctatc
    10321 ttgataagga tattcagaat cagaatgaga aattaaaagc ggctcaggca tctttaaacg
    10381 caatgaatga atccttatcc agagataagg ctgcacttac cggggcgatg gagagccgga
    10441 aacaaaagga gaaaaaagcg aaggatgcag aaaataagtt aaatgaggaa aagaacaagc
    10501 ctcgcaaggg gactaaagac tacggccatg attatcatcc agtacctaaa acagaagaca
    10561 taaagggact tggagagtta aagcgaggtg atcctaaaac acctaaacaa ggtggtggtg
    10621 gtaagcgggc ccgatggtat ggcgataaaa aacgtaaaat ttatgaatgg gattcccagc
    10681 acggtgagct tgaagggtat cgtgccagtg atggcgaaca cctaggcgca tttgatccca
    10741 aaacgggtaa gcaggttaaa gggccagatc caaaacggaa cattaaaaaa tatctttgag
    10801 aggtaaatat ggggcttaaa ttacgtttga catggtttga taagaaaact gaagagttta
    10861 aaggggaaga gtattctaaa gactttggtg acgatggttc tgtcattgaa agcttgggaa
    10921 tgcctttaaa ggataatatt aataatggtg agtttgattt gaagaaagaa tgggttcctt
    10981 tactacagcc attttttaaa aatcaaataa aacctgatgt ggataattgt tatatagctt
    11041 ttgattatcg ggacggtaac tggtaacaaa aaattatcaa ggatgatttg aaatactgac
    11101 ttccggtgtt cagggatgaa tgccggagtc tttaaataca gggaaagtat atgaaaaagg
    11161 taaagactat ttttcttttt atattgattg ttgctggctt tcttctggcc gcatgtcagg
    11221 caaattatat ccgggatgtt cagggcggaa cggtggcacc atcgtcctcc tctgaactga
    11281 cggggatcgc ggttcagtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    11341 cgtaatcttt tgccctgtaa acgaaaaaac cacctgggga ggtggtttga tcgaaggtta
    11401 agtcagttgg ggaactgctt aaccgggtaa ctggctttcg cagagcacag caaccaaatc
    11461 tgtccttcca gtgtagccgg actttggcgc acacttcaag agcaaccgcg tgtttagcta
    11521 aacaaatcct ctgcgaactc ccagttacca atggctgctg ccagtggcgt tttgcgtgct
    11581 ttcccgggtt ggactcaagt gaacagttac cggataaggc gcagcagtcg ggctgaacgg
    11641 ggagttcttg cttacagccc agcttggagc gaacgaccta caccgagccg agataccagt
    11701 gtgtgagcta tgagaaagcg ccacacttcc cgcaagggag aacggcggac aggtatccgg
    11761 taaacggcag ggtcggaaca ggagagcgca agagggagcg acctaccgga aacggtgggg
    11821 atctttaagt cctgtcgggt ttcgcccgta ctgtcagatt catggttgag cctcacggct
    11881 cccacagatg caccgaaaaa gcgtctgttt atgtgaactc tggcaggagg gcggagccta
    11941 tggaaaaacg ccaccggcgc ggccctgcc
//
