LOCUS       NZ_CP147163             5596 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain SC42 plasmid p4, complete sequence.
ACCESSION   NZ_CP147163
VERSION     NZ_CP147163.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40144599
            Assembly: GCF_037157715.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Wang,D.
  TITLE     Klebsiella pneumoniae strains whole genome sequences
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Wang,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2024) School of Life Sciences, Taizhou University,
            1139 Shifudadao Avenue, Taizhou, Zhejiang 318000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.15.3; Canu v. 2.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina NovaSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037157715.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 18:41:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,636
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,373
            CDSs (with protein)               :: 5,373
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 50 of 140
            Pseudo Genes (incomplete)         :: 93 of 140
            Pseudo Genes (internal stop)      :: 17 of 140
            Pseudo Genes (multiple problems)  :: 19 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147163.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SC42"
                     /isolation_source="Wound discharge"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p4"
                     /geo_loc_name="China: Taizhou"
                     /lat_lon="28.40 N 121.26 E"
                     /collection_date="2021-07-16"
                     /collected_by="Dongguo Wang"
     gene            complement(join(5492..5596,1..120))
                     /locus_tag="WBS28_RS28095"
     CDS             complement(join(5492..5596,1..120))
                     /locus_tag="WBS28_RS28095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            283..411
                     /locus_tag="WBS28_RS28100"
     CDS             283..411
                     /locus_tag="WBS28_RS28100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            complement(787..999)
                     /locus_tag="WBS28_RS28105"
     CDS             complement(787..999)
                     /locus_tag="WBS28_RS28105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1006..1515)
                     /locus_tag="WBS28_RS28110"
     CDS             complement(1006..1515)
                     /locus_tag="WBS28_RS28110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1550..2254)
                     /locus_tag="WBS28_RS28115"
                     /pseudo=""
     CDS             complement(<1550..2254)
                     /locus_tag="WBS28_RS28115"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2193..2537)
                     /locus_tag="WBS28_RS28120"
     CDS             complement(2193..2537)
                     /locus_tag="WBS28_RS28120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2527..2703)
                     /locus_tag="WBS28_RS28125"
     CDS             complement(2527..2703)
                     /locus_tag="WBS28_RS28125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            3343..3501
                     /locus_tag="WBS28_RS28130"
     CDS             3343..3501
                     /locus_tag="WBS28_RS28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            3859..4185
                     /locus_tag="WBS28_RS28135"
     CDS             3859..4185
                     /locus_tag="WBS28_RS28135"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            4211..4756
                     /locus_tag="WBS28_RS28140"
     CDS             4211..4756
                     /locus_tag="WBS28_RS28140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            4822..5172
                     /locus_tag="WBS28_RS28145"
     CDS             4822..5172
                     /locus_tag="WBS28_RS28145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(5242..5508)
                     /locus_tag="WBS28_RS28150"
     CDS             complement(5242..5508)
                     /locus_tag="WBS28_RS28150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          complement(2790..2866)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          3055..3452
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(672..2203)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caggtcttcc agatgcgcca gtattgcttc ccgcgcataa aacgtctttg ttctgccggt
       61 ttgcttcgcc agcgagtcca gacgggactc aatctcatca gataaccgga tagcaagcat
      121 ataaacctcc cgaacaccat ttgctataca tgtatatcaa cagtaccaca atcaacctaa
      181 aagaacaaaa aataatcttg ttagcccctc ttgataatcc gtaacgcggt caatatatct
      241 agcttgaggg ctttcctctt aactattgaa aggataacga ttatgggtct taagccgggt
      301 ccgaaacgaa ttgccgattc aacaggggaa cctgataaac gccagcgtga caataaaaaa
      361 acgccgggaa atactgacaa attgaaaccc agcaaatctt caaaaaaata agtcagtagt
      421 taaaagccct ccggggcttt tttgtatctg ccgttcacgc tgattaaaca taacacccgt
      481 tgcccgcacc gccccggacg ggttcgccgc gaacggggtg gcagtcaggt aaaaccggca
      541 acaaaccacc cgataaaccg ccccgttcgc gacagataaa ttttacctgg ctgcatagcc
      601 atgcagccag gtgaatgaca tccggtttca gcgtaaatgc ccgatgagaa ccctgttttc
      661 ctgcatttca gcccagcgac ggaccgtcat agcttcgctg ccgggaacgc gcttccgtca
      721 gttccttttc gtgctgccgt tgctcgtgcg cccgttctgc ggccacggcc atctcatgac
      781 gctgcactac cggctcaaac gctgcagttg cccgctcaag cgttccacct gctgactcaa
      841 gcgcgtcacg cgctcgctca gcgccgcgtt ctcccgttgc ataagccctg acattttccg
      901 ccattccccg aaggcgctct cccactcgtc cagccttttc gagtagtcct gctgtagctg
      961 ctctaatgcg ctcagcaact gtttttccag ctcggtcatc ggcacttact ccttcaccag
     1021 tctcagcgcg ttctgcttcg accgcccgtt cccgaccgtc cagctctgcg tcctgtctac
     1081 ggccgtcccc ttcggcagca ccagaaaacg tccgttgctg tcttccaggt acgtcacgcc
     1141 ccaggtcttc gcgttcagct tcgacagcgc tgcgttctgc cggtcgattt cggccagatt
     1201 tgacgctatc aagctcccct gataccacaa caccccgctc aggctggcga acagcagtcc
     1261 tgacaccatc gtgatggtca gccatgtccg cccgaccata cgcatcacgc tcaggcggtt
     1321 cgactgcatg gccgcgctca taccctgctc gtgggcgcgg atggcggcgc tgattctctt
     1381 cccgctttcg ctcagttccg ctctgacaga cttctcgtgc tcgctgaatg cggctttcag
     1441 catttcgccg gtactctgct gctgcgcttt cgatttctgc tctaagtcct tcgccagcgt
     1501 taaaagactg ttcatagatg gctcccttaa gtcggatgtt tcgccctccg tccgggtcgg
     1561 caatgctgat gctgctcttt gtggtgcgca cgacctcaaa acctgcgctt tccagcgcct
     1621 cagtgacgtc ctgacgcgtt ttaagctccc ctgacgaggc gagggccagt aagccccgcg
     1681 taatagcctc tgcggcttcc tgcttcgttt tgggtaacga agatggcgtc accagcgcgc
     1741 gccggttctc cggtgcgttc gggtcgtgca gccccagtcg cccgttcacc acggtctgcc
     1801 acgcatcgat gcgcggacgg tctgcccggt cgtagtacgg ctggagacgc ctgccggtca
     1861 gcagttccgt gttcggtacc agaaaattca gttccagccg ccccttgtcc cggtgttcaa
     1921 cccacagcac gctgtactgg tctttatcga gtccgggcat cagaacccgc tcgaagctcg
     1981 ccatcagctt ttcacgctgg ccgggcggta aatcctgttc agcaaaagac aggacgccgg
     2041 aggtgtactt tttggcgtag ggcgaggcgt cgataagctc ccggacctcc tccggctttc
     2101 cctgcaggac gatggccccg tcgcgctggc ggtctttccc cagcagataa tccaccggcc
     2161 cggcaccgcc gcctcgccct cgcggatgaa acttaacgat catcgtctgc ccccttttcc
     2221 agcacggcat gccgcagccg ctcgagttcg gcatcaatgg ccatcagcgc ggccaccacc
     2281 tgaacccggt cagcgcctga ccactggccg ccgttaattt tccgggcgat ctggttgagg
     2341 ttgttcccca tcccggcaag ctggcgcagc aaaaccggat cgatggacgg cagccgcaac
     2401 gaccgcgccg ggcgcgtatc gagacaggtt tcccgcatcc acaccgccag ttgccttcca
     2461 ttgcagcgct ccagcagctg ctggtactcc ccatcggtca cccggatacc taaaaaacgg
     2521 gtacgcttaa ccgccatcct ctcctccgat atccgcaaaa tgtgaccggg ctgaacgctc
     2581 aagctctgcc atctgctccc ggcggtaatc ttcctcctgt gcctgacgca tcagatttgc
     2641 catcactgcc ggatgccagg atcttttttt tacgacgagc ggcttcttct tcggcttctg
     2701 cattatccct ccttcgcaaa cgccggacgg cacggaaaag ttttccgggt cgctccggcg
     2761 ttggacaagc gccagcgcgt cagtggtgtt agcgggtttc ggggcgcagc cctgaaccag
     2821 tcacagcgtt tacgctgtgt atactggctt actatgttgg catgagagca cgattcatga
     2881 aagtgcctca tgtggcaggt gtgatatgtg gctcaggagc gtccgcaata ccatgcacgg
     2941 acagatgaaa tgacgcctcc tcgctcactc ggtagctgcg ctcctgccgt tcggctgcgg
     3001 cgggcgtgac cgactcacgg ataacgggat aaagcaaagg cgctcaaatc agaaagggcc
     3061 ggagaaaagg atgcggcgtt gccgtttttc cataggctcc gcctcccctg acgagcatca
     3121 cagaaatgga cgctcaagtc agaggaggac gaaacccgac aggacttaaa gatgccggtg
     3181 atttctctcc ggcggctccc tcgtgcgctc tcctgttccg accctgccgt ttaccggata
     3241 cctgttccgc ctttttccct ttcgggaagc gtggcgcttt ctcatagctc acgctgctgg
     3301 tatctcggct cggtttaggt cgttcgctcc aagctgggct gtatgcacga accccccgtt
     3361 cagcccgact gctgcgccct ttccggtaac tatcatcttg agtccaaccc ggacggacac
     3421 gcaaaaacgc cactggcagc agccattggt aactggatat atacgagaga gatattgaga
     3481 ggtcttgaag tgggtgccta actgcggcta cactgaaaga acagttttgg tatctgcggt
     3541 ctcgtatacc cagttacccg gttaagcagt tccccaactg acttaacctt cgatcaaacc
     3601 gcctccccag gcggtttttt cgtttacagt gcaagagatt acgcgcagaa aaaaaggatc
     3661 tcaagaagat cctttaaatg atcgtcagtt ctgttatccc cggagctcca tccatcccac
     3721 ggaaaaacac ccgccactga tgccctcagc gcactgcaca tcatgtacaa accactccct
     3781 ccctcctttt ttattgcgtt tcttaatgcg caatggtagc tttgtgcgca acaaaaaacg
     3841 caatagcaag ggggggtaat gagagaaaga acaaatatca tgctagatat aggattaaag
     3901 caatccttac aacggctttc gaataaaacc aaaatgagtc taagtgatct tattaaccag
     3961 agattatcag gttcactttc agttgaagaa cgcattggcg aaaagcctga atggatgaga
     4021 aaccttgatc cctatctcgc caatcacttt tcaaatattg agttaccgct cccttcacca
     4081 ttaaaagaat accaagaaaa aatggatgag atagattcaa ttattaatga catgatggtt
     4141 ctaaaggaat atttcagagc taagttttac gacgaaataa aataggcacg ctatacggag
     4201 gcggagaatt atgtttttta aacggtattt aacggagaag gaaatataca aggcagcact
     4261 cccaattgtt aggcttcaag ggagagttaa cacttcatta cgttttttat ccaaatactc
     4321 agatgtaaac aaaaatggat taattgcatt gcatcttaat ttattacatt ttatctcata
     4381 caacatacac ggtgtagagt ctctgaaaat cactcaagca ggcataaaca aagctgtaca
     4441 acaaagccct cacgcagatg aaataaaaaa tattgcgcag gcaataggac gcaaaggacc
     4501 tgagtacatc gaaggattaa ttagaaaccc atgggggaag aaaaatgata atccactaat
     4561 atttataatg agcattgaag aaagtataaa agaaaccctt aactcaggtt atatgttata
     4621 tagagtagct cccataactg agggtgaagc agctgctttc atggatgttc ttaaacaatt
     4681 attattattt gaggataatt ttcgcctttt tggtagagac ttggtttata aaatagaaaa
     4741 attaaaaatc agataacaaa gtatggtgaa cgaaaaatga aagagaggat agtgaaatgt
     4801 gtaataccaa gattctcaac tatgtattgg ttaattgcgt tcctagcatt tatattcccc
     4861 ggtcgatctt ttgctgacgg ttacaccgct tactaccgat gtaatggtac tcaggaagca
     4921 tctcttactg ttaaagataa caaactaagt atggttgtag gtctttctaa gtttaatagg
     4981 caactggata cttatagatt caaagaaaag ggaatcgaag tagtaatgca gagagctgct
     5041 tctgatgata actctgaggc cataatgtct tttattatgc ctgacgttaa caatatgacc
     5101 ttattagtta aaaataacgg ctacattatc gttaagacaa cctgctggtt aactaaaacc
     5161 gacaaacagt agaacacgta gttagtcgag tatttaccat aatacctgtt gtcggcatca
     5221 aaaaagcccg catagcgggc ttcagtcgta aacttcgcgg cgatgtccaa tcgtggcggc
     5281 aaggataaca agctcgtcat ctcggatctc acacagaacg cgataatctc ccacccgata
     5341 gcgccagaac tcgcccagct cacctttgag cggcttccct gactggcgag gatcggcagc
     5401 aactgcaatg cgtaaactca taaaatccac aatccgtcgt gcgttctgtt tgtccatctt
     5461 gcgtaacgat ttgagcgccc gatcggaata gttaatcgtc cagaccaagt gacttcctca
     5521 cgtcttcaga cgaatgcact gcttcatcac cacggcgaac gcgtgcagca gtttctgctg
     5581 aaagataata atcctc
//
