LOCUS       NZ_CP147162            10060 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain SC42 plasmid p3, complete sequence.
ACCESSION   NZ_CP147162
VERSION     NZ_CP147162.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40144599
            Assembly: GCF_037157715.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Wang,D.
  TITLE     Klebsiella pneumoniae strains whole genome sequences
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Wang,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2024) School of Life Sciences, Taizhou University,
            1139 Shifudadao Avenue, Taizhou, Zhejiang 318000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.15.3; Canu v. 2.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina NovaSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037157715.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 18:41:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,636
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,373
            CDSs (with protein)               :: 5,373
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 50 of 140
            Pseudo Genes (incomplete)         :: 93 of 140
            Pseudo Genes (internal stop)      :: 17 of 140
            Pseudo Genes (multiple problems)  :: 19 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147162.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SC42"
                     /isolation_source="Wound discharge"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p3"
                     /geo_loc_name="China: Taizhou"
                     /lat_lon="28.40 N 121.26 E"
                     /collection_date="2021-07-16"
                     /collected_by="Dongguo Wang"
     gene            complement(join(9846..10060,1..43))
                     /locus_tag="WBS28_RS28030"
     CDS             complement(join(9846..10060,1..43))
                     /locus_tag="WBS28_RS28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(53..1738)
                     /locus_tag="WBS28_RS28035"
     CDS             complement(53..1738)
                     /locus_tag="WBS28_RS28035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            2065..2310
                     /locus_tag="WBS28_RS28040"
     CDS             2065..2310
                     /locus_tag="WBS28_RS28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            2584..2955
                     /gene="tolA"
                     /locus_tag="WBS28_RS28045"
     CDS             2584..2955
                     /gene="tolA"
                     /locus_tag="WBS28_RS28045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            2952..3317
                     /locus_tag="WBS28_RS28050"
     CDS             2952..3317
                     /locus_tag="WBS28_RS28050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            3492..3770
                     /locus_tag="WBS28_RS28055"
     CDS             3492..3770
                     /locus_tag="WBS28_RS28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            3758..4072
                     /locus_tag="WBS28_RS28060"
     CDS             3758..4072
                     /locus_tag="WBS28_RS28060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4236..4664
                     /locus_tag="WBS28_RS28065"
     CDS             4236..4664
                     /locus_tag="WBS28_RS28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            4690..4869
                     /locus_tag="WBS28_RS28070"
     CDS             4690..4869
                     /locus_tag="WBS28_RS28070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4896..5426)
                     /locus_tag="WBS28_RS28075"
     CDS             complement(4896..5426)
                     /locus_tag="WBS28_RS28075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5433..6164)
                     /gene="mobC"
                     /locus_tag="WBS28_RS28080"
     CDS             complement(5433..6164)
                     /gene="mobC"
                     /locus_tag="WBS28_RS28080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(6164..8128)
                     /locus_tag="WBS28_RS28085"
     CDS             complement(6164..8128)
                     /locus_tag="WBS28_RS28085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(9612..9761)
                     /locus_tag="WBS28_RS28090"
     CDS             complement(9612..9761)
                     /locus_tag="WBS28_RS28090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1..6738
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          8931..9509
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5437..6164)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 cagttttctt atcaaaccat gtcaaacgta atttaagccc catatttacc tctcaaagat
       61 attttttaat gttccgtttt ggatctggcc ctttaacctg cttacccgtt ttgggatcaa
      121 atgcgcctag gtgttcgcca tcactggcac gatacccttc aagctcaccg tgctgggaat
      181 cccattcata aattttacgt tttttatcgc cataccatcg ggcccgctta ccaccaccac
      241 cttgtttagg tgttttagga tcacctcgct ttaactctcc aagtcccttt atgtcttctg
      301 ttttaggtac tggatgataa tcatggccgt agtctttagt ccccttgcga ggcttgttct
      361 tttcctcatt taacttattt tctgcatcct tcgctttttt ctccttttgt ttccggctct
      421 ccatcgcccc ggtaagtgca gccttatctc tggataagga ttcattcatt gcgtttaaag
      481 atgcctgagc cgcttttaat ttctcattct gattctgaat atccttatca agatagcgaa
      541 cctgatcctg ccacccggag ccatattcat gaggcgggtt ataagtcaca aaatcatctt
      601 tatttgcctc tgcctctttt acctttttat cggcttcctg aacagcagcc cttgcaccgg
      661 ggagcgcctt ctctgtcgat gcaatgcggc tgtttaaggt cgcaatattt ttatcttcgg
      721 catccagctc agctttcgct ttatcatagt ctcttttcag cccctcttcc ggatgagcgg
      781 cgtcccatgc ctgctggcga cgcttttctt cctcctgacg ctgttttacc tgtgccgggg
      841 taagaacatc tgtcaccgac acataaaccg gcttctgtcc ggtctctttc gggaaacgaa
      901 taacggcctc acgggagtta ccaccggctg taaaaccagc cggacgtgaa tcaccttttt
      961 cagcaacaat gccttttggc ggggctttcg ctgtcggaac acctttcggt acgctcacct
     1021 gcagagacgg gagacccgga atcgacacac tgaatacccc cggacgtttt gtcggtttcg
     1081 catccaccac agggacactc attggccgac ctgcgacaac cgcaatatgc tgccgctcat
     1141 ccttcaccac atccacaacc cgttgtctga cacgaaccgt cgcctggtcc agtggtaaag
     1201 tacttgccgg agtctccgtc accgcatcgg ccggtaatga cgtcataatt tttgacatca
     1261 ttttcggatc atcttttgca atctcagaag gcagcacacc gtagatcgca atcccccaca
     1321 gaccaaactt aaacggcccc ttcagggcag ccagcacatc agcaacggcg gccgacaacg
     1381 catcgccgga aactgataaa gccagtcctt cagcccccgg agtagccagg gcaggcagac
     1441 cgaaggccat cgcagtggcc gaagactgtc cgccaccaga gctaccgcta tgattgccgc
     1501 tgttaccacc attaccaccg ggtttactcc cgccgttacc aaactccccc ggattatagt
     1561 tatgaatatc tccattcggc gtatgcgtcg taccccagcc cggcccgctg tttggatccg
     1621 taccaccacc tgatgaaggg ccacctttac cagacgtacc gttcacattc ccactggcct
     1681 gaccaccatt atgtcccaga cctgaattac ccggacctcg accgtctcca ccactcatac
     1741 cgttttcctc tttgacattt aaaacaataa attaaagcca acatactgta catatacaca
     1801 gtataattat taccttactc ctcccgtttt caggtgtcaa tatatgagat tagttctcat
     1861 cttcaggcac aaaaaagcgc ccggagccac ctgtaagcag atcgcgtctt ttcccgaaaa
     1921 tttctcatgg caacaaattt tattatttaa gacattcatc acacttacat ttcagatggt
     1981 atagacacat ctgaaagcta actggcatga ggagaggacc tgcggttaaa ctgttcggga
     2041 aacctgagac gcccggaggc cactatgacg cacctgaaac tgactcccgc tgaagaaaag
     2101 cgcctggcgg acgcctttgt cgctcaggca ctggccatgg gcctgaaagg gaacaaaacg
     2161 ctgccaaagg cggtaaaaca acaggtgcgg gaaagggtgc ttcgtgccat tcaggacgaa
     2221 cgtgaagcca ggcgggcgca ggaaatgcgt cgggagaaat acgaggctac ggaaaccacc
     2281 ttcacctggc agccacagcg ccgccggtaa ggggtattta ccataacgtc gcattgcccg
     2341 caccgctcag acggtctgag ggcgtttccg gcggctacgg gataaaacag gcaacaaacc
     2401 acccggaaaa ccgccggaaa cgcgcccggt aaattgtaac ccgctgcatg cctatgcacg
     2461 caactgcatg gcgggccgtt tggtgtaaaa acgatacgat aagccgtgtt tatgtaaatg
     2521 aacgtggccg taaagagtga caaatataca tgtaacattt tttactgaca ggtcatcaac
     2581 ccgatgcgtt taataccctt tatcctgctg gcattgtgca gccccttact cctgacggcc
     2641 tgtaagcatt ccaccaccca gccgccgaaa atttatactc tggataagtc atctaaaatc
     2701 agtagctacg ctgcggccat caagaccgct gttgaaatgc agatgtttga accagaggcc
     2761 tatgcgggaa aaatgtgcac cgtgcggata tccatacaac gtgatggcac tctgaacagc
     2821 gccattgctg aaggaggaga tccgaaactc tgtaaagcag caatatcagc cgttacccac
     2881 gccaaaattc cgccggcacc ggatgaaaat acctggcaaa tattcaaaaa cgcccctctg
     2941 gatttcagac catgaaagct tttactctga cagtgctggc ggccatgttc ctcactccgg
     3001 tacatgcgat accactaaaa cccgtaacca cactgcagcc aacctacccg gtacaggccg
     3061 cagcaaaacg catcgaaggt caggtgaccg ccacgtttga tattctggac gatggacgag
     3121 tggacaacgt gtcgcttacc ggctgtcacc ttttttttcg ggaaacgctg acggcaatga
     3181 ggcactggct atatcccgca gggcacccgg caatgcgaca ggttataaat tttgaattca
     3241 gtctgaaaaa tgacggctta caggatccgc gaccagtcac cacccaaaag ctcaatgatg
     3301 atggttgccg ccagtaacag gtcctttaac ccgcaggctg caatagtcct ggatgatgag
     3361 tgagcctgta gcagccctga ttagtggaag cgaagctgaa aaatgggcag caaaagccgt
     3421 atttctggca aatagcgccg ccacggatta aagtagtcat gtgtaataca tatgactaca
     3481 aaggggcttt tatgtcatca gcaacatcca tcaggctcga tgaagaactt aaggaccggc
     3541 tgaaaacgct ggccgatgac cggcaccgct cagcgcacgc gctaatgctg gaggcaatca
     3601 ccgaatacat cgaccgggaa gagaagcgca gccagtacct gcgggacgga caggccgcat
     3661 ggcagcacta tcaggaaacg ggtctgcacc ttactgcgga agaagcggaa gcctggataa
     3721 gtacctgggg aactgagaac gagcaggatg ctccgccatg ccacaggtaa cgatttccgc
     3781 gctggcacag cgcgatttac aacgccttca ggactttctc aaaaccaaaa atcggctggc
     3841 agccagaaaa ggaggtgagg tgatcgtccg ggctatccag caactgaaaa cattgccaga
     3901 cattggccgc ccggtgccgt ttctgccgct ggaatataag gaactggtga tcgggtttgg
     3961 cgacagtggc tatgtgatgc tctaccgcca cgaccgggaa atggaccaga ttgtgatcgt
     4021 caccgtcagg catcagaaag aatccgggta tccgggggca gacagcctct aataaccgat
     4081 gacgggggcg caattcattg cgtggccgtc gccggtttga caagcgcagc gcgtcagaaa
     4141 accacatcct gaacggaaaa acgggtaaaa ttcagcgata catttaccgt gagaaattac
     4201 acacctttca cacccgataa aatcaggggt atacagtggc gaaaacattg ctcaaccttt
     4261 cacaggcagc acaagccgcg ggcattacca gaagaacgct atacaatcat gtaaaacagg
     4321 gaaaggttac cgtgtcacgt gacgggaaaa acaaccctgt cgtggatgtg tcagaactga
     4381 tccgtgtata cggaaatgtg aatatccctg agaaacaaat tcccggcatt tcacaccgtg
     4441 aaaatacaca aaaaaatttc ccacaggaac agcttctggc aatgcaaaag gaactggccg
     4501 atctccggca ggctgtcacc ctgatgctgg aagataaaac atcccgggaa gaagaacgcc
     4561 gccagcacga tgatgagcgc cggaaactcc aggccgaagt ggacagattg acaacagaac
     4621 tgacacagaa gaaaaaacgc ttctggtccg gctggtttag ctgagaactt accgaaggga
     4681 atggagaaca tgacgaagca ggaaaaaact gccctcaata tggctaaatt cattaaggat
     4741 cagtcgctgc tgttgctgga aaagctgaac gagctggaca tggatacccc ggccgacatg
     4801 tgcgaacggc ttcacgagga tgcggaaaca ctctggcagg tgatgcggga taagctcggg
     4861 gaagaataac agcaccggcc atgccggtgc tggcgttatt ttttgccgaa cggccaccac
     4921 cggcgggcgg gtttgtcctc cggcagttca ggcaccggca cgttaaaata gcggctgtta
     4981 tattcccgca tcagctccgc tttcagcttc tcgttcaccg cgctttcccc gtgccgctcg
     5041 ctgaccacct caatcatcgc gttaatctgc tccggtgagc acttcccggc atcaaccacc
     5101 tgcacaccgt tctcgatggc gaaccgctcc acctttcgat agaggtaatc acacagcttc
     5161 attcctgtgg gtttgtcccg ttcccattgc ggattttcac cggtgaaaaa gtacgtggct
     5221 ttttcgtcgc tgttgagccg ctcgtcatac atcccgatgg atttcatcat cgccagatgc
     5281 tgggcccggt cgacaaaaca gaaaacatac gccccggtct gccaggcgag caccaccatg
     5341 tcttccagat gctcgctggt tggcataatg cagcgctcgc cgtctttgtc ttccacgtcc
     5401 tcaataaata ccatgtacag cgccataatc cctcactccg ttgtcataaa tttaaagcac
     5461 gcgaacagcc tctcccggtc cagctccacc gttttgcggg cctcgccggt ctgtggattc
     5521 acgatttcat ccaccgccag aatcgcccgc tgtacccgga caagcgtcgg ccggtcaggg
     5581 cagatgtacc agatttcatc gatgccgttc gcccggcggt tgaacagata cccggacacc
     5641 acttcctgat accgcctgcg gctcttgacg gtgcgctcta cctcaaatgc caccgttctg
     5701 ccgtccagct ccgccagcgc gtccggcact ttcatccctt tcagggccat acggtgcagg
     5761 taccgccagc cggtggcccc ccggccctcc atggagaggc gcagggactg cacggccagc
     5821 cggtgcggca gctgcgccgc cgacaccctt gacggctgaa acggaataac gtccgtcagc
     5881 ggctcgtcct cgtcgaacga gaacgccacc ccgtgcggcg tcagccccca cacgctgacc
     5941 tgacggccgt caaccgggta tttcgcccgg accagcagcc ctttcgccac cagactgtcc
     6001 aggaagcggt aaaccggctg taccgccgca atccccagcc gtcgcgcaac cagctccgcc
     6061 gtggaacaca gctcttcccg cagccagcgc agggtcagcc gtgttttctc cgcgttccgc
     6121 gcctgccgct tcgcgtggct gacgttgtac tgctccagtg tcatcagtaa agccccgcaa
     6181 aatcatcatc cgcttttggt ccgccgccgt cggccggagg caggtactcc gccagcgggt
     6241 ccgttttcgc cagcaccggg aaggttttca gcgaaatttc ccggcgctca acccgcagcg
     6301 ggctgctgaa cgccagccgc gccacgccca gcccggtcat caccgcgaac ccgtcaggca
     6361 ggtgttgcag ggtgttcacg tcaaacagcg gccgcgcctc cttctggatg aagctgtcgc
     6421 cgctgatgtg ctccacgttc cccgcctccc ggctggcccg gcggcgctcc acgtccaccc
     6481 ggatctcccc ggtctggccc gccgcccact gcgcggactc ctggctggcc gcgcgataga
     6541 accagcggat cggcgtgtta tccagtaccg tggttttcac aaaatcagcg ggcagatcct
     6601 gcccgcactg accgaagtcg cccagcgact ggtgcgcgag cagcaggttg cagtcccggc
     6661 tgcgcagcgt ccccagcgcg ttcagcacgt acttcgacag cagatattta atctcatcga
     6721 gcatgatgct cgcgtgcggc cacctccgga actcatcccg cgcaatgatg atttgcgcgc
     6781 agcgggcaaa cagcatcttc tgcacccgga tcaccgcctc atcgtccatc gagccgatga
     6841 cgtacaggca gccgccgccg ttgattatcc ccgccacatc gataccgctg tccgtctgca
     6901 ggacgctcag gtcggccacg ttctccagct tggtgatcag ccccttcacg tcctttttca
     6961 gggcctccgg caacgcgtac gccgccgcca gcacctgctg aatgtttgcc ccctgcggga
     7021 actgctccgc aatcagcttc gccgctttct gctcgctgat gcggtagtgg tccgccacgt
     7081 cgccggtctc actcaggtta aacccggcaa ccagcagatt cttcagggcg tactggtcga
     7141 gatcgcggaa cagattcagc tgcgccggtt tccccggccg cagatccagc agggtgaagt
     7201 ccgggcagtg cgcccggaag acgtgcggcg cccacgcgtc ccctttgggg tcgaagtaca
     7261 ccacagcgtc cccgaaatcg cgtacgcagc gaatgagcgc gttggtggcc atcgcgctct
     7321 tgccgctccc gggcaggccg aggatctgaa tattggtctt gcgccaggta tcacgaggga
     7381 ccagcaccgg ctggccctgc tcatccttac ccagaaacac cgcctgctgt ttccgggcct
     7441 gctccgtgga aaaatcggcc agcgcgttaa ccggtaccgg ctccccgaaa tgcaggtggc
     7501 ggacgtctgt catttctccg gtccgctggc tgctgcgccg gctctcctga ttcagtttcg
     7561 cgttcaccgc ctcgtcagcg gccgcaaaat gaccatcaag cagcaggaac gccagcagcc
     7621 gcgccagaag gacgccggcg acaagccctc cggcacacca ggctatcccg aacaggttag
     7681 ccggctgcag caggtaataa tctgcgttcg catagaggtc cgggcgcagg acggtttctc
     7741 cccagctctc gataaacacg gcgacaacca gcccccccag aagggccaga ccggcagcgc
     7801 cggtcagcca gtacgcgtag ttccagacat gtcgccgtgc cctgaccatc cgcccgggtg
     7861 aatccagcga ggccagcacc gaaaatggca cagtccggtt attatcgcgc ttatagcgat
     7921 cggcatactc ccgccgcgcc ttatggctca gccccacaaa acgcattcgg ccggcccaga
     7981 gttcagccag accgaaaaac aggccgataa ccagaaaaaa accggccaga ctgtaatact
     8041 cccggtacag ccagctgacc cacgggtaaa tctgccgggc cagctccggg atatgccact
     8101 gccaggcaag cgaatccgta ttaaacatgc aatctctctc cttcagacga cgggtttacc
     8161 ggcgtgatgc gcagcccccg ctgcacaagc cgcggacatg ccccaaggca tggcggcggc
     8221 ggtcacgtcc gtggtgattg cgttcagctt tcaccgcgga ggccagcctc tttccgcccc
     8281 atgcggaaaa ggcgcagccc cggaggaaca acgagcacgc cccagcgtgt gagaggaaaa
     8341 ggcggcaccc cagtgcggtc ttttccggtg ttccggcccc cgtcagagtt cgccatagcg
     8401 agcggctgac cacccgcaaa atcaggagca gctttgctgc ttcgtcaggt gcgtccagca
     8461 ccgtggattt ttgtgggtgg tcaggcgtga gcctgacggg ggccgggtga atttctggcg
     8521 tggccggcgt ccagccggac agagccagaa agaggggaaa agaccgtgcg tcggtggcgt
     8581 ccagccagcg taagcaaggt cttttcccct gaagtcagcc acgccccagc ggggatgact
     8641 tgcatttctc cgcaaggaga aaaaagcaac agagataggt taacgtaaaa tgttcagcga
     8701 aaaaccgaca tggttcacct atcctgataa ttgatcgtca gggaatagaa agacttaatc
     8761 aggggacaat atcccacatc agcgcgtccg gtgaccgtct tgcaggtgcc ggggaaattg
     8821 cgcctcctcg ctcactcggt cgctacgctc cgggcgtgag actgcggcgg gcgctgcgga
     8881 cacatacaaa gttacccaca gattccgtgg ataagcaggg gactaacatg tgaggcaaaa
     8941 cggcagggcc gcgccggtgg cgtttttcca taggctccgc cctcctgcca gagttcacat
     9001 aaacagacgc tttttcggtg catctgtggg agccgtgagg ctcaaccatg aatctgacag
     9061 tacgggcgaa acccgacagg acttaaagat ccccaccgtt tccggtaggt cgctccctct
     9121 tgcgctctcc tgttccgacc ctgccgttta ccggatacct gtccgccgtt ctcccttgcg
     9181 ggaagtgtgg cgctttctca tagctcacac actggtatct cggctcggtg taggtcgttc
     9241 gctccaagct gggctgtaag caagaactcc ccgttcagcc cgactgctgc gccttatccg
     9301 gtaactgttc acttgagtcc aacccgggaa agcacgcaaa acgccactgg cagcagccat
     9361 tggtaactgg gagttcgcag aggatttgtt tagctaaaca cgcggttgct cttgaagtgt
     9421 gcgccaaagt ccggctacac tggaaggaca gatttggttg ctgtgctctg cgaaagccag
     9481 ttaccacggt taagcagttc cccaactgac ttaaccttcg atcaaaccac ctccccaggt
     9541 ggttttttcg tttacagggc aaaagattac gcgcagaaaa aaaggatctc aagaagatcc
     9601 tttgatcttt tctactgaac cgcgatcccc gtcagttcag aggaggacga tggtgccacc
     9661 gttccgccct gaacatcccg gatataattt gcctgacatg cggccagaag aaagccagca
     9721 acaatcaata taaaaagaaa aatagtcttt acctttttca tatactttcc ctgtatttaa
     9781 agactccggc attcatccct gaacaccgga agtcagtatt tcaaatcatc cttgataatt
     9841 ttttgttacc agttaccgtc ccgataatca aaagctatat aacaattatc cacatcaggt
     9901 tttatttgat ttttaaaaaa tggctgtagt aaaggaaccc attctttctt caaatcaaac
     9961 tcaccattat taatattatc ctttaaaggc attcccaagc tttcaatgac agaaccatcg
    10021 tcaccaaagt ctttagaata ctcttcccct ttaaactctt
//
