LOCUS       NZ_CP147158             5596 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain SC67 plasmid p3, complete sequence.
ACCESSION   NZ_CP147158
VERSION     NZ_CP147158.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40144600
            Assembly: GCF_037157705.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Wang,D.
  TITLE     Klebsiella pneumoniae strains whole genome sequences
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Wang,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2024) School of Life Sciences, Taizhou University,
            1139 Shifudadao Avenue, Taizhou, Zhejiang 318000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.15.3; Canu v. 2.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina NovaSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037157705.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 18:29:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,627
            CDSs (total)                      :: 5,506
            Genes (coding)                    :: 5,362
            CDSs (with protein)               :: 5,362
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 45 of 144
            Pseudo Genes (incomplete)         :: 100 of 144
            Pseudo Genes (internal stop)      :: 15 of 144
            Pseudo Genes (multiple problems)  :: 16 of 144
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147158.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SC67"
                     /isolation_source="clean catch midstream urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p3"
                     /geo_loc_name="China: Taizhou"
                     /lat_lon="28.40 N 121.26 E"
                     /collection_date="2021-12-27"
                     /collected_by="Dongguo Wang"
     gene            complement(join(5472..5596,1..220))
                     /locus_tag="WBS31_RS28080"
     CDS             complement(join(5472..5596,1..220))
                     /locus_tag="WBS31_RS28080"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(210..386)
                     /locus_tag="WBS31_RS28085"
     CDS             complement(210..386)
                     /locus_tag="WBS31_RS28085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            1026..1184
                     /locus_tag="WBS31_RS28090"
     CDS             1026..1184
                     /locus_tag="WBS31_RS28090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1542..1868
                     /locus_tag="WBS31_RS28095"
     CDS             1542..1868
                     /locus_tag="WBS31_RS28095"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            1894..2439
                     /locus_tag="WBS31_RS28100"
     CDS             1894..2439
                     /locus_tag="WBS31_RS28100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            2505..2855
                     /locus_tag="WBS31_RS28105"
     CDS             2505..2855
                     /locus_tag="WBS31_RS28105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2925..3191)
                     /locus_tag="WBS31_RS28110"
     CDS             complement(2925..3191)
                     /locus_tag="WBS31_RS28110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3175..3399)
                     /locus_tag="WBS31_RS28115"
     CDS             complement(3175..3399)
                     /locus_tag="WBS31_RS28115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            3562..3690
                     /locus_tag="WBS31_RS28120"
     CDS             3562..3690
                     /locus_tag="WBS31_RS28120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            complement(4066..4278)
                     /locus_tag="WBS31_RS28125"
     CDS             complement(4066..4278)
                     /locus_tag="WBS31_RS28125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4285..4794)
                     /locus_tag="WBS31_RS28130"
     CDS             complement(4285..4794)
                     /locus_tag="WBS31_RS28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4829..5533)
                     /locus_tag="WBS31_RS28135"
                     /pseudo=""
     CDS             complement(<4829..5533)
                     /locus_tag="WBS31_RS28135"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(473..549)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          738..1135
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(3951..5482)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttttccgggc gatctggttg aggttgttcc ccatcccggc aagctggcgc agcaaaaccg
       61 gatcgatgga cggcagccgc aacgaccgcg ccgggcgcgt atcgagacag gtttcccgca
      121 tccacaccgc cagttgcctt ccattgcagc gctccagcag ctgctggtac tccccatcgg
      181 tcacccggat acctaaaaaa cgggtacgct taaccgccat cctctcctcc gatatccgca
      241 aaatgtgacc gggctgaacg ctcaagctct gccatctgct cccggcggta atcttcctcc
      301 tgtgcctgac gcatcagatt tgccatcact gccggatgcc aggatctttt ttttacgacg
      361 agcggcttct tcttcggctt ctgcattatc cctccttcgc aaacgccgga cggcacggaa
      421 aagttttccg ggtcgctccg gcgttggaca agcgccagcg cgtcagtggt gttagcgggt
      481 ttcggggcgc agccctgaac cagtcacagc gtttacgctg tgtatactgg cttactatgt
      541 tggcatgaga gcacgattca tgaaagtgcc tcatgtggca ggtgtgatat gtggctcagg
      601 agcgtccgca ataccatgca cggacagatg aaatgacgcc tcctcgctca ctcggtagct
      661 gcgctcctgc cgttcggctg cggcgggcgt gaccgactca cggataacgg gataaagcaa
      721 aggcgctcaa atcagaaagg gccggagaaa aggatgcggc gttgccgttt ttccataggc
      781 tccgcctccc ctgacgagca tcacagaaat ggacgctcaa gtcagaggag gacgaaaccc
      841 gacaggactt aaagatgccg gtgatttctc tccggcggct ccctcgtgcg ctctcctgtt
      901 ccgaccctgc cgtttaccgg atacctgttc cgcctttttc cctttcggga agcgtggcgc
      961 tttctcatag ctcacgctgc tggtatctcg gctcggttta ggtcgttcgc tccaagctgg
     1021 gctgtatgca cgaacccccc gttcagcccg actgctgcgc cctttccggt aactatcatc
     1081 ttgagtccaa cccggacgga cacgcaaaaa cgccactggc agcagccatt ggtaactgga
     1141 tatatacgag agagatattg agaggtcttg aagtgggtgc ctaactgcgg ctacactgaa
     1201 agaacagttt tggtatctgc ggtctcgtat acccagttac ccggttaagc agttccccaa
     1261 ctgacttaac cttcgatcaa accgcctccc caggcggttt tttcgtttac agtgcaagag
     1321 attacgcgca gaaaaaaagg atctcaagaa gatcctttaa atgatcgtca gttctgttat
     1381 ccccggagct ccatccatcc cacggaaaaa cacccgccac tgatgccctc agcgcactgc
     1441 acatcatgta caaaccactc cctccctcct tttttattgc gtttcttaat gcgcaatggt
     1501 agctttgtgc gcaacaaaaa acgcaatagc aagggggggt aatgagagaa agaacaaata
     1561 tcatgctaga tataggatta aagcaatcct tacaacggct ttcgaataaa accaaaatga
     1621 gtctaagtga tcttattaac cagagattat caggttcact ttcagttgaa gaacgcattg
     1681 gcgaaaagcc tgaatggatg agaaaccttg atccctatct cgccaatcac ttttcaaata
     1741 ttgagttacc gctcccttca ccattaaaag aataccaaga aaaaatggat gagatagatt
     1801 caattattaa tgacatgatg gttctaaagg aatatttcag agctaagttt tacgacgaaa
     1861 taaaataggc acgctatacg gaggcggaga attatgtttt ttaaacggta tttaacggag
     1921 aaggaaatat acaaggcagc actcccaatt gttaggcttc aagggagagt taacacttca
     1981 ttacgttttt tatccaaata ctcagatgta aacaaaaatg gattaattgc attgcatctt
     2041 aatttattac attttatctc atacaacata cacggtgtag agtctctgaa aatcactcaa
     2101 gcaggcataa acaaagctgt acaacaaagc cctcacgcag atgaaataaa aaatattgcg
     2161 caggcaatag gacgcaaagg acctgagtac atcgaaggat taattagaaa cccatggggg
     2221 aagaaaaatg ataatccact aatatttata atgagcattg aagaaagtat aaaagaaacc
     2281 cttaactcag gttatatgtt atatagagta gctcccataa ctgagggtga agcagctgct
     2341 ttcatggatg ttcttaaaca attattatta tttgaggata attttcgcct ttttggtaga
     2401 gacttggttt ataaaataga aaaattaaaa atcagataac aaagtatggt gaacgaaaaa
     2461 tgaaagagag gatagtgaaa tgtgtaatac caagattctc aactatgtat tggttaattg
     2521 cgttcctagc atttatattc cccggtcgat cttttgctga cggttacacc gcttactacc
     2581 gatgtaatgg tactcaggaa gcatctctta ctgttaaaga taacaaacta agtatggttg
     2641 taggtctttc taagtttaat aggcaactgg atacttatag attcaaagaa aagggaatcg
     2701 aagtagtaat gcagagagct gcttctgatg ataactctga ggccataatg tcttttatta
     2761 tgcctgacgt taacaatatg accttattag ttaaaaataa cggctacatt atcgttaaga
     2821 caacctgctg gttaactaaa accgacaaac agtagaacac gtagttagtc gagtatttac
     2881 cataatacct gttgtcggca tcaaaaaagc ccgcatagcg ggcttcagtc gtaaacttcg
     2941 cggcgatgtc caatcgtggc ggcaaggata acaagctcgt catctcggat ctcacacaga
     3001 acgcgataat ctcccacccg atagcgccag aactcgccca gctcaccttt gagcggcttc
     3061 cctgactggc gaggatcggc agcaactgca atgcgtaaac tcataaaatc cacaatccgt
     3121 cgtgcgttct gtttgtccat cttgcgtaac gatttgagcg cccgatcgga atagttaatc
     3181 gtccagacca agtgacttcc tcacgtcttc agacgaatgc actgcttcat caccacggcg
     3241 aacgcgtgca gcagtttctg ctgaaagata ataatcctcc aggtcttcca gatgcgccag
     3301 tattgcttcc cgcgcataaa acgtctttgt tctgccggtt tgcttcgcca gcgagtccag
     3361 acgggactca atctcatcag ataaccggat agcaagcata taaacctccc gaacaccatt
     3421 tgctatacat gtatatcaac agtaccacaa tcaacctaaa agaacaaaaa ataatcttgt
     3481 tagcccctct tgataatccg taacgcggtc aatatatcta gcttgagggc tttcctctta
     3541 actattgaaa ggataacgat tatgggtctt aagccgggtc cgaaacgaat tgccgattca
     3601 acaggggaac ctgataaacg ccagcgtgac aataaaaaaa cgccgggaaa tactgacaaa
     3661 ttgaaaccca gcaaatcttc aaaaaaataa gtcagtagtt aaaagccctc cggggctttt
     3721 ttgtatctgc cgttcacgct gattaaacat aacacccgtt gcccgcaccg ccccggacgg
     3781 gttcgccgcg aacggggtgg cagtcaggta aaaccggcaa caaaccaccc gataaaccgc
     3841 cccgttcgcg acagataaat tttacctggc tgcatagcca tgcagccagg tgaatgacat
     3901 ccggtttcag cgtaaatgcc cgatgagaac cctgttttcc tgcatttcag cccagcgacg
     3961 gaccgtcata gcttcgctgc cgggaacgcg cttccgtcag ttccttttcg tgctgccgtt
     4021 gctcgtgcgc ccgttctgcg gccacggcca tctcatgacg ctgcactacc ggctcaaacg
     4081 ctgcagttgc ccgctcaagc gttccacctg ctgactcaag cgcgtcacgc gctcgctcag
     4141 cgccgcgttc tcccgttgca taagccctga cattttccgc cattccccga aggcgctctc
     4201 ccactcgtcc agccttttcg agtagtcctg ctgtagctgc tctaatgcgc tcagcaactg
     4261 tttttccagc tcggtcatcg gcacttactc cttcaccagt ctcagcgcgt tctgcttcga
     4321 ccgcccgttc ccgaccgtcc agctctgcgt cctgtctacg gccgtcccct tcggcagcac
     4381 cagaaaacgt ccgttgctgt cttccaggta cgtcacgccc caggtcttcg cgttcagctt
     4441 cgacagcgct gcgttctgcc ggtcgatttc ggccagattt gacgctatca agctcccctg
     4501 ataccacaac accccgctca ggctggcgaa cagcagtcct gacaccatcg tgatggtcag
     4561 ccatgtccgc ccgaccatac gcatcacgct caggcggttc gactgcatgg ccgcgctcat
     4621 accctgctcg tgggcgcgga tggcggcgct gattctcttc ccgctttcgc tcagttccgc
     4681 tctgacagac ttctcgtgct cgctgaatgc ggctttcagc atttcgccgg tactctgctg
     4741 ctgcgctttc gatttctgct ctaagtcctt cgccagcgtt aaaagactgt tcatagatgg
     4801 ctcccttaag tcggatgttt cgccctccgt ccgggtcggc aatgctgatg ctgctctttg
     4861 tggtgcgcac gacctcaaaa cctgcgcttt ccagcgcctc agtgacgtcc tgacgcgttt
     4921 taagctcccc tgacgaggcg agggccagta agccccgcgt aatagcctct gcggcttcct
     4981 gcttcgtttt gggtaacgaa gatggcgtca ccagcgcgcg ccggttctcc ggtgcgttcg
     5041 ggtcgtgcag ccccagtcgc ccgttcacca cggtctgcca cgcatcgatg cgcggacggt
     5101 ctgcccggtc gtagtacggc tggagacgcc tgccggtcag cagttccgtg ttcggtacca
     5161 gaaaattcag ttccagccgc cccttgtccc ggtgttcaac ccacagcacg ctgtactggt
     5221 ctttatcgag tccgggcatc agaacccgct cgaagctcgc catcagcttt tcacgctggc
     5281 cgggcggtaa atcctgttca gcaaaagaca ggacgccgga ggtgtacttt ttggcgtagg
     5341 gcgaggcgtc gataagctcc cggacctcct ccggctttcc ctgcaggacg atggccccgt
     5401 cgcgctggcg gtctttcccc agcagataat ccaccggccc ggcaccgccg cctcgccctc
     5461 gcggatgaaa cttaacgatc atcgtctgcc cccttttcca gcacggcatg ccgcagccgc
     5521 tcgagttcgg catcaatggc catcagcgcg gccaccacct gaacccggtc agcgcctgac
     5581 cactggccgc cgttaa
//
