LOCUS       NZ_CP147157            10059 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain SC67 plasmid p2, complete sequence.
ACCESSION   NZ_CP147157
VERSION     NZ_CP147157.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40144600
            Assembly: GCF_037157705.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10059)
  AUTHORS   Wang,D.
  TITLE     Klebsiella pneumoniae strains whole genome sequences
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10059)
  AUTHORS   Wang,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2024) School of Life Sciences, Taizhou University,
            1139 Shifudadao Avenue, Taizhou, Zhejiang 318000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.15.3; Canu v. 2.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina NovaSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037157705.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 18:29:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,627
            CDSs (total)                      :: 5,506
            Genes (coding)                    :: 5,362
            CDSs (with protein)               :: 5,362
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 45 of 144
            Pseudo Genes (incomplete)         :: 100 of 144
            Pseudo Genes (internal stop)      :: 15 of 144
            Pseudo Genes (multiple problems)  :: 16 of 144
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147157.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10059
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SC67"
                     /isolation_source="clean catch midstream urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2"
                     /geo_loc_name="China: Taizhou"
                     /lat_lon="28.40 N 121.26 E"
                     /collection_date="2021-12-27"
                     /collected_by="Dongguo Wang"
     gene            complement(join(9411..10059,1..1316))
                     /locus_tag="WBS31_RS28015"
     CDS             complement(join(9411..10059,1..1316))
                     /locus_tag="WBS31_RS28015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(2799..2948)
                     /locus_tag="WBS31_RS28020"
     CDS             complement(2799..2948)
                     /locus_tag="WBS31_RS28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(3033..3290)
                     /locus_tag="WBS31_RS28025"
     CDS             complement(3033..3290)
                     /locus_tag="WBS31_RS28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(3300..4985)
                     /locus_tag="WBS31_RS28030"
     CDS             complement(3300..4985)
                     /locus_tag="WBS31_RS28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5312..5557
                     /locus_tag="WBS31_RS28035"
     CDS             5312..5557
                     /locus_tag="WBS31_RS28035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            5831..6202
                     /gene="tolA"
                     /locus_tag="WBS31_RS28040"
     CDS             5831..6202
                     /gene="tolA"
                     /locus_tag="WBS31_RS28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            6199..6564
                     /locus_tag="WBS31_RS28045"
     CDS             6199..6564
                     /locus_tag="WBS31_RS28045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            6739..7017
                     /locus_tag="WBS31_RS28050"
     CDS             6739..7017
                     /locus_tag="WBS31_RS28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            7005..7319
                     /locus_tag="WBS31_RS28055"
     CDS             7005..7319
                     /locus_tag="WBS31_RS28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            7483..7911
                     /locus_tag="WBS31_RS28060"
     CDS             7483..7911
                     /locus_tag="WBS31_RS28060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            7937..8116
                     /locus_tag="WBS31_RS28065"
     CDS             7937..8116
                     /locus_tag="WBS31_RS28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8143..8673)
                     /locus_tag="WBS31_RS28070"
     CDS             complement(8143..8673)
                     /locus_tag="WBS31_RS28070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8680..9411)
                     /gene="mobC"
                     /locus_tag="WBS31_RS28075"
     CDS             complement(8680..9411)
                     /gene="mobC"
                     /locus_tag="WBS31_RS28075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     BGC             1..9985
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2119..2696
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.11"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8684..9411)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 accgcctcat cgtccatcga gccgatgacg tacaggcagc cgccgccgtt gattatcccc
       61 gccacatcga taccgctgtc cgtctgcagg acgctcaggt cggccacgtt ctccagcttg
      121 gtgatcagcc ccttcacgtc ctttttcagg gcctccggca acgcgtacgc cgccgccagc
      181 acctgctgaa tgtttgcccc ctgcgggaac tgctccgcaa tcagcttcgc cgctttctgc
      241 tcgctgatgc ggtagtggtc cgccacgtcg ccggtctcac tcaggttaaa cccggcaacc
      301 agcagattct tcagggcgta ctggtcgaga tcgcggaaca gattcagctg cgccggtttc
      361 cccggccgca gatccagcag ggtgaagtcc gggcagtgcg cccggaagac gtgcggcgcc
      421 cacgcgtccc ctttggggtc gaagtacacc acagcgtccc cgaaatcgcg tacgcagcga
      481 atgagcgcgt tggtggccat cgcgctcttg ccgctcccgg gcaggccgag gatctgaata
      541 ttggtcttgc gccaggtatc acgagggacc agcaccggct ggccctgctc atccttaccc
      601 agaaacaccg cctgctgttt ccgggcctgc tccgtggaaa aatcggccag cgcgttaacc
      661 ggtaccggct ccccgaaatg caggtggcgg acgtctgtca tttctccggt ccgctggctg
      721 ctgcgccggc tctcctgatt cagtttcgcg ttcaccgcct cgtcagcggc cgcaaaatga
      781 ccatcaagca gcaggaacgc cagcagccgc gccagaagga cgccggcgac aagccctccg
      841 gcacaccagg ctatcccgaa caggttagcc ggctgcagca ggtaataatc tgcgttcgca
      901 tagaggtccg ggcgcaggac ggtttctccc cagctctcga taaacacggc gacaaccagc
      961 ccccccagaa gggccagacc ggcagcgccg gtcagccagt acgcgtagtt ccagacatgt
     1021 cgccgtgccc tgaccatccg cccgggtgaa tccagcgagg ccagcaccga aaatggcaca
     1081 gtccggttat tatcgcgctt atagcgatcg gcatactccc gccgcgcctt atggctcagc
     1141 cccacaaaac gcattcggcc ggcccagagt tcagccagac cgaaaaacag gccgataacc
     1201 agaaaaaaac cggccagact gtaatactcc cggtacagcc agctgaccca cgggtaaatc
     1261 tgccgggcca gctccgggat atgccactgc caggcaagcg aatccgtatt aaacatgcaa
     1321 tctctctcct tcagacgacg ggtttaccgg cgtgatgcgc agcccccgct gcacaagccg
     1381 cggacatgcc ccaaggcatg gcggcggcgg tcacgtccgt ggtgattgcg ttcagctttc
     1441 accgcggagg ccagcctctt tccgccccat gcggaaaagg cgcagccccg gaggaacaac
     1501 gagcacgccc cagcgtgtga gaggaaaagg cggcacccca gtgcggtctt ttccggtgtt
     1561 ccggcccccg tcagagttcg ccatagcgag cggctgacca cccgcaaaat caggagcagc
     1621 tttgctgctt cgtcaggtgc gtccagcacc gtggattttt gtgggtggtc aggcgtgagc
     1681 ctgacggggg ccgggtgaat ttctggcgtg gccggcgtcc agccggacag agccagaaag
     1741 aggggaaaag accgtgcgtc ggtggcgtcc agccagcgta agcaaggtct tttcccctga
     1801 agtcagccac gccccagcgg ggatgacttg catttctccg caaggagaaa aaagcaacag
     1861 agataggtta acgtaaaatg ttcagcgaaa aaccgacatg gttcacctat cctgataatt
     1921 gatcgtcagg gaatagaaag acttaatcag gggacaatat cccacatcag cgcgtccggt
     1981 gaccgtcttg caggtgccgg ggaaattgcg cctcctcgct cactcggtcg ctacgctccg
     2041 ggcgtgagac tgcggcgggc gctgcggaca catacaaagt tacccacaga ttccgtggat
     2101 aagcagggga ctaacatgtg aggcaaaacg gcagggccgc gccggtggcg tttttccata
     2161 ggctccgccc tcctgccaga gttcacataa acagacgctt tttcggtgca tctgtgggag
     2221 ccgtgaggct caaccatgaa tctgacagta cgggcgaaac ccgacaggac ttaaagatcc
     2281 ccaccgtttc cggtaggtcg ctccctcttg cgctctcctg ttccgaccct gccgtttacc
     2341 ggatacctgt ccgccgttct cccttgcggg aagtgtggcg ctttctcata gctcacacac
     2401 tggtatctcg gctcggtgta ggtcgttcgc tccaagctgg gctgtaagca agaactcccc
     2461 gttcagcccg actgctgcgc cttatccggt aactgttcac ttgagtccaa cccgggaaag
     2521 cacgcaaaac gccactggca gcagccattg gtaactggga gttcgcagag gatttgttta
     2581 gctaaacacg cggttgctct tgaagtgtgc gccaaagtcc ggctacactg gaaggacaga
     2641 tttggttgct gtgctctgcg aaagccagtt acccggttaa gcagttcccc aactgactta
     2701 accttcgatc aaaccacctc cccaggtggt tttttcgttt acagggcaaa agattacgcg
     2761 cagaaaaaaa ggatctcaag aagatccttt gatcttttct actgaaccgc gatccccgtc
     2821 agttcagagg aggacgatgg tgccaccgtt ccgccctgaa catcccggat ataatttgcc
     2881 tgacatgcgg ccagaagaaa gccagcaaca atcaatataa aaagaaaaat agtctttacc
     2941 tttttcatat actttccctg tatttaaaga ctccggcatt catccctgaa caccggaagt
     3001 cagtatttca aatcatcctt gataattttt tgttaccagt taccgtcccg ataatcaaaa
     3061 gctatataac aattatccac atcaggtttt atttgatttt taaaaaatgg ctgtagtaaa
     3121 ggaacccatt ctttcttcaa atcaaactca ccattattaa tattatcctt taaaggcatt
     3181 cccaagcttt caatgacaga accatcgtca ccaaagtctt tagaatactc ttccccttta
     3241 aactcttcag ttttcttatc aaaccatgtc aaacgtaatt taagccccat atttacctct
     3301 caaagatatt ttttaatgtt ccgttttgga tctggccctt taacctgctt acccgttttg
     3361 ggatcaaatg cgcctaggtg ttcgccatca ctggcacgat acccttcaag ctcaccgtgc
     3421 tgggaatccc attcataaat tttacgtttt ttatcgccat accatcgggc ccgcttacca
     3481 ccaccacctt gtttaggtgt tttaggatca cctcgcttta actctccaag tccctttatg
     3541 tcttctgttt taggtactgg atgataatca tggccgtagt ctttagtccc cttgcgaggc
     3601 ttgttctttt cctcatttaa cttattttct gcatccttcg cttttttctc cttttgtttc
     3661 cggctctcca tcgccccggt aagtgcagcc ttatctctgg ataaggattc attcattgcg
     3721 tttaaagatg cctgagccgc ttttaatttc tcattctgat tctgaatatc cttatcaaga
     3781 tagcgaacct gatcctgcca cccggagcca tattcatgag gcgggttata agtcacaaaa
     3841 tcatctttat ttgcctctgc ctcttttacc tttttatcgg cttcctgaac agcagccctt
     3901 gcaccgggga gcgccttctc tgtcgatgca atgcggctgt ttaaggtcgc aatattttta
     3961 tcttcggcat ccagctcagc tttcgcttta tcatagtctc ttttcagccc ctcttccgga
     4021 tgagcggcgt cccatgcctg ctggcgacgc ttttcttcct cctgacgctg ttttacctgt
     4081 gccggggtaa gaacatctgt caccgacaca taaaccggct tctgtccggt ctctttcggg
     4141 aaacgaataa cggcctcacg ggagttacca ccggctgtaa aaccagccgg acgtgaatca
     4201 cctttttcag caacaatgcc ttttggcggg gctttcgctg tcggaacacc tttcggtacg
     4261 ctcacctgca gagacgggag acccggaatc gacacactga atacccccgg acgttttgtc
     4321 ggtttcgcat ccaccacagg gacactcatt ggccgacctg cgacaaccgc aatatgctgc
     4381 cgctcatcct tcaccacatc cacaacccgt tgtctgacac gaaccgtcgc ctggtccagt
     4441 ggtaaagtac ttgccggagt ctccgtcacc gcatcggccg gtaatgacgt cataattttt
     4501 gacatcattt tcggatcatc ttttgcaatc tcagaaggca gcacaccgta gatcgcaatc
     4561 ccccacagac caaacttaaa cggccccttc agggcagcca gcacatcagc aacggcggcc
     4621 gacaacgcat cgccggaaac tgataaagcc agtccttcag cccccggagt agccagggca
     4681 ggcagaccga aggccatcgc agtggccgaa gactgtccgc caccagagct accgctatga
     4741 ttgccgctgt taccaccatt accaccgggt ttactcccgc cgttaccaaa ctcccccgga
     4801 ttatagttat gaatatctcc attcggcgta tgcgtcgtac cccagcccgg cccgctgttt
     4861 ggatccgtac caccacctga tgaagggcca cctttaccag acgtaccgtt cacattccca
     4921 ctggcctgac caccattatg tcccagacct gaattacccg gacctcgacc gtctccacca
     4981 ctcataccgt tttcctcttt gacatttaaa acaataaatt aaagccaaca tactgtacat
     5041 atacacagta taattattac cttactcctc ccgttttcag gtgtcaatat atgagattag
     5101 ttctcatctt caggcacaaa aaagcgcccg gagccacctg taagcagatc gcgtcttttc
     5161 ccgaaaattt ctcatggcaa caaattttat tatttaagac attcatcaca cttacatttc
     5221 agatggtata gacacatctg aaagctaact ggcatgagga gaggacctgc ggttaaactg
     5281 ttcgggaaac ctgagacgcc cggaggccac tatgacgcac ctgaaactga ctcccgctga
     5341 agaaaagcgc ctggcggacg cctttgtcgc tcaggcactg gccatgggcc tgaaagggaa
     5401 caaaacgctg ccaaaggcgg taaaacaaca ggtgcgggaa agggtgcttc gtgccattca
     5461 ggacgaacgt gaagccaggc gggcgcagga aatgcgtcgg gagaaatacg aggctacgga
     5521 aaccaccttc acctggcagc cacagcgccg ccggtaaggg gtatttacca taacgtcgca
     5581 ttgcccgcac cgctcagacg gtctgagggc gtttccggcg gctacgggat aaaacaggca
     5641 acaaaccacc cggaaaaccg ccggaaacgc gcccggtaaa ttgtaacccg ctgcatgcct
     5701 atgcacgcaa ctgcatggcg ggccgtttgg tgtaaaaacg atacgataag ccgtgtttat
     5761 gtaaatgaac gtggccgtaa agagtgacaa atatacatgt aacatttttt actgacaggt
     5821 catcaacccg atgcgtttaa taccctttat cctgctggca ttgtgcagcc ccttactcct
     5881 gacggcctgt aagcattcca ccacccagcc gccgaaaatt tatactctgg ataagtcatc
     5941 taaaatcagt agctacgctg cggccatcaa gaccgctgtt gaaatgcaga tgtttgaacc
     6001 agaggcctat gcgggaaaaa tgtgcaccgt gcggatatcc atacaacgtg atggcactct
     6061 gaacagcgcc attgctgaag gaggagatcc gaaactctgt aaagcagcaa tatcagccgt
     6121 tacccacgcc aaaattccgc cggcaccgga tgaaaatacc tggcaaatat tcaaaaacgc
     6181 ccctctggat ttcagaccat gaaagctttt actctgacag tgctggcggc catgttcctc
     6241 actccggtac atgcgatacc actaaaaccc gtaaccacac tgcagccaac ctacccggta
     6301 caggccgcag caaaacgcat cgaaggtcag gtgaccgcca cgtttgatat tctggacgat
     6361 ggacgagtgg acaacgtgtc gcttaccggc tgtcaccttt tttttcggga aacgctgacg
     6421 gcaatgaggc actggctata tcccgcaggg cacccggcaa tgcgacaggt tataaatttt
     6481 gaattcagtc tgaaaaatga cggcttacag gatccgcgac cagtcaccac ccaaaagctc
     6541 aatgatgatg gttgccgcca gtaacaggtc ctttaacccg caggctgcaa tagtcctgga
     6601 tgatgagtga gcctgtagca gccctgatta gtggaagcga agctgaaaaa tgggcagcaa
     6661 aagccgtatt tctggcaaat agcgccgcca cggattaaag tagtcatgtg taatacatat
     6721 gactacaaag gggcttttat gtcatcagca acatccatca ggctcgatga agaacttaag
     6781 gaccggctga aaacgctggc cgatgaccgg caccgctcag cgcacgcgct aatgctggag
     6841 gcaatcaccg aatacatcga ccgggaagag aagcgcagcc agtacctgcg ggacggacag
     6901 gccgcatggc agcactatca ggaaacgggt ctgcacctta ctgcggaaga agcggaagcc
     6961 tggataagta cctggggaac tgagaacgag caggatgctc cgccatgcca caggtaacga
     7021 tttccgcgct ggcacagcgc gatttacaac gccttcagga ctttctcaaa accaaaaatc
     7081 ggctggcagc cagaaaagga ggtgaggtga tcgtccgggc tatccagcaa ctgaaaacat
     7141 tgccagacat tggccgcccg gtgccgtttc tgccgctgga atataaggaa ctggtgatcg
     7201 ggtttggcga cagtggctat gtgatgctct accgccacga ccgggaaatg gaccagattg
     7261 tgatcgtcac cgtcaggcat cagaaagaat ccgggtatcc gggggcagac agcctctaat
     7321 aaccgatgac gggggcgcaa ttcattgcgt ggccgtcgcc ggtttgacaa gcgcagcgcg
     7381 tcagaaaacc acatcctgaa cggaaaaacg ggtaaaattc agcgatacat ttaccgtgag
     7441 aaattacaca cctttcacac ccgataaaat caggggtata cagtggcgaa aacattgctc
     7501 aacctttcac aggcagcaca agccgcgggc attaccagaa gaacgctata caatcatgta
     7561 aaacagggaa aggttaccgt gtcacgtgac gggaaaaaca accctgtcgt ggatgtgtca
     7621 gaactgatcc gtgtatacgg aaatgtgaat atccctgaga aacaaattcc cggcatttca
     7681 caccgtgaaa atacacaaaa aaatttccca caggaacagc ttctggcaat gcaaaaggaa
     7741 ctggccgatc tccggcaggc tgtcaccctg atgctggaag ataaaacatc ccgggaagaa
     7801 gaacgccgcc agcacgatga tgagcgccgg aaactccagg ccgaagtgga cagattgaca
     7861 acagaactga cacagaagaa aaaacgcttc tggtccggct ggtttagctg agaacttacc
     7921 gaagggaatg gagaacatga cgaagcagga aaaaactgcc ctcaatatgg ctaaattcat
     7981 taaggatcag tcgctgctgt tgctggaaaa gctgaacgag ctggacatgg ataccccggc
     8041 cgacatgtgc gaacggcttc acgaggatgc ggaaacactc tggcaggtga tgcgggataa
     8101 gctcggggaa gaataacagc accggccatg ccggtgctgg cgttattttt tgccgaacgg
     8161 ccaccaccgg cgggcgggtt tgtcctccgg cagttcaggc accggcacgt taaaatagcg
     8221 gctgttatat tcccgcatca gctccgcttt cagcttctcg ttcaccgcgc tttccccgtg
     8281 ccgctcgctg accacctcaa tcatcgcgtt aatctgctcc ggtgagcact tcccggcatc
     8341 aaccacctgc acaccgttct cgatggcgaa ccgctccacc tttcgataga ggtaatcaca
     8401 cagcttcatt cctgtgggtt tgtcccgttc ccattgcgga ttttcaccgg tgaaaaagta
     8461 cgtggctttt tcgtcgctgt tgagccgctc gtcatacatc ccgatggatt tcatcatcgc
     8521 cagatgctgg gcccggtcga caaaacagaa aacatacgcc ccggtctgcc aggcgagcac
     8581 caccatgtct tccagatgct cgctggttgg cataatgcag cgctcgccgt ctttgtcttc
     8641 cacgtcctca ataaatacca tgtacagcgc cataatccct cactccgttg tcataaattt
     8701 aaagcacgcg aacagcctct cccggtccag ctccaccgtt ttgcgggcct cgccggtctg
     8761 tggattcacg atttcatcca ccgccagaat cgcccgctgt acccggacaa gcgtcggccg
     8821 gtcagggcag atgtaccaga tttcatcgat gccgttcgcc cggcggttga acagataccc
     8881 ggacaccact tcctgatacc gcctgcggct cttgacggtg cgctctacct caaatgccac
     8941 cgttctgccg tccagctccg ccagcgcgtc cggcactttc atccctttca gggccatacg
     9001 gtgcaggtac cgccagccgg tggccccccg gccctccatg gagaggcgca gggactgcac
     9061 ggccagccgg tgcggcagct gcgccgccga cacccttgac ggctgaaacg gaataacgtc
     9121 cgtcagcggc tcgtcctcgt cgaacgagaa cgccaccccg tgcggcgtca gcccccacac
     9181 gctgacctga cggccgtcaa ccgggtattt cgcccggacc agcagccctt tcgccaccag
     9241 actgtccagg aagcggtaaa ccggctgtac cgccgcaatc cccagccgtc gcgcaaccag
     9301 ctccgccgtg gaacacagct cttcccgcag ccagcgcagg gtcagccgtg ttttctccgc
     9361 gttccgcgcc tgccgcttcg cgtggctgac gttgtactgc tccagtgtca tcagtaaagc
     9421 cccgcaaaat catcatccgc ttttggtccg ccgccgtcgg ccggaggcag gtactccgcc
     9481 agcgggtccg ttttcgccag caccgggaag gttttcagcg aaatttcccg gcgctcaacc
     9541 cgcagcgggc tgctgaacgc cagccgcgcc acgcccagcc cggtcatcac cgcgaacccg
     9601 tcaggcaggt gttgcagggt gttcacgtca aacagcggcc gcgcctcctt ctggatgaag
     9661 ctgtcgccgc tgatgtgctc cacgttcccc gcctcccggc tggcccggcg gcgctccacg
     9721 tccacccgga tctccccggt ctggcccgcc gcccactgcg cggactcctg gctggccgcg
     9781 cgatagaacc agcggatcgg cgtgttatcc agtaccgtgg ttttcacaaa atcagcgggc
     9841 agatcctgcc cgcactgacc gaagtcgccc agcgactggt gcgcgagcag caggttgcag
     9901 tcccggctgc gcagcgtccc cagcgcgttc agcacgtact tcgacagcag atatttaatc
     9961 tcatcgagca tgatgctcgc gtgcggccac ctccggaact catcccgcgc aatgatgatt
    10021 tgcgcgcagc gggcaaacag catcttctgc acccggatc
//
