LOCUS       NZ_CP147154             5596 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain SC173 plasmid p3, complete sequence.
ACCESSION   NZ_CP147154
VERSION     NZ_CP147154.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40144601
            Assembly: GCF_037157695.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Wang,D.
  TITLE     Klebsiella pneumoniae strains whole genome sequences
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Wang,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2024) School of Life Sciences, Taizhou University,
            1139 Shifudadao Avenue, Taizhou, Zhejiang 318000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.15.3; Canu v. 2.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina NovaSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037157695.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 18:40:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,476
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 45 of 129
            Pseudo Genes (incomplete)         :: 85 of 129
            Pseudo Genes (internal stop)      :: 15 of 129
            Pseudo Genes (multiple problems)  :: 16 of 129
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147154.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SC173"
                     /isolation_source="biliary fluid drainage"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p3"
                     /geo_loc_name="China: Taizhou"
                     /lat_lon="28.40 N 121.26 E"
                     /collection_date="2020-09-04"
                     /collected_by="Dongguo Wang"
     gene            complement(join(5366..5596,1..114))
                     /locus_tag="WBS27_RS27940"
     CDS             complement(join(5366..5596,1..114))
                     /locus_tag="WBS27_RS27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(104..280)
                     /locus_tag="WBS27_RS27945"
     CDS             complement(104..280)
                     /locus_tag="WBS27_RS27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            920..1078
                     /locus_tag="WBS27_RS27950"
     CDS             920..1078
                     /locus_tag="WBS27_RS27950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1436..1762
                     /locus_tag="WBS27_RS27955"
     CDS             1436..1762
                     /locus_tag="WBS27_RS27955"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            1788..2333
                     /locus_tag="WBS27_RS27960"
     CDS             1788..2333
                     /locus_tag="WBS27_RS27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            2399..2749
                     /locus_tag="WBS27_RS27965"
     CDS             2399..2749
                     /locus_tag="WBS27_RS27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2819..3085)
                     /locus_tag="WBS27_RS27970"
     CDS             complement(2819..3085)
                     /locus_tag="WBS27_RS27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3069..3293)
                     /locus_tag="WBS27_RS27975"
     CDS             complement(3069..3293)
                     /locus_tag="WBS27_RS27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(3960..4172)
                     /locus_tag="WBS27_RS27980"
     CDS             complement(3960..4172)
                     /locus_tag="WBS27_RS27980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4179..4688)
                     /locus_tag="WBS27_RS27985"
     CDS             complement(4179..4688)
                     /locus_tag="WBS27_RS27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4723..5427)
                     /locus_tag="WBS27_RS27990"
                     /pseudo=""
     CDS             complement(<4723..5427)
                     /locus_tag="WBS27_RS27990"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(367..443)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          632..1029
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(3845..5376)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acaggtttcc cgcatccaca ccgccagttg ccttccattg cagcgctcca gcagctgctg
       61 gtactcccca tcggtcaccc ggatacctaa aaaacgggta cgcttaaccg ccatcctctc
      121 ctccgatatc cgcaaaatgt gaccgggctg aacgctcaag ctctgccatc tgctcccggc
      181 ggtaatcttc ctcctgtgcc tgacgcatca gatttgccat cactgccgga tgccaggatc
      241 ttttttttac gacgagcggc ttcttcttcg gcttctgcat tatccctcct tcgcaaacgc
      301 cggacggcac ggaaaagttt tccgggtcgc tccggcgttg gacaagcgcc agcgcgtcag
      361 tggtgttagc gggtttcggg gcgcagccct gaaccagtca cagcgtttac gctgtgtata
      421 ctggcttact atgttggcat gagagcacga ttcatgaaag tgcctcatgt ggcaggtgtg
      481 atatgtggct caggagcgtc cgcaatacca tgcacggaca gatgaaatga cgcctcctcg
      541 ctcactcggt agctgcgctc ctgccgttcg gctgcggcgg gcgtgaccga ctcacggata
      601 acgggataaa gcaaaggcgc tcaaatcaga aagggccgga gaaaaggatg cggcgttgcc
      661 gtttttccat aggctccgcc tcccctgacg agcatcacag aaatggacgc tcaagtcaga
      721 ggaggacgaa acccgacagg acttaaagat gccggtgatt tctctccggc ggctccctcg
      781 tgcgctctcc tgttccgacc ctgccgttta ccggatacct gttccgcctt tttccctttc
      841 gggaagcgtg gcgctttctc atagctcacg ctgctggtat ctcggctcgg tttaggtcgt
      901 tcgctccaag ctgggctgta tgcacgaacc ccccgttcag cccgactgct gcgccctttc
      961 cggtaactat catcttgagt ccaacccgga cggacacgca aaaacgccac tggcagcagc
     1021 cattggtaac tggatatata cgagagagat attgagaggt cttgaagtgg gtgcctaact
     1081 gcggctacac tgaaagaaca gttttggtat ctgcggtctc gtatacccag ttacccggtt
     1141 aagcagttcc ccaactgact taaccttcga tcaaaccgcc tccccaggcg gttttttcgt
     1201 ttacagtgca agagattacg cgcagaaaaa aaggatctca agaagatcct ttaaatgatc
     1261 gtcagttctg ttatccccgg agctccatcc atcccacgga aaaacacccg ccactgatgc
     1321 cctcagcgca ctgcacatca tgtacaaacc actccctccc tcctttttta ttgcgtttct
     1381 taatgcgcaa tggtagcttt gtgcgcaaca aaaaacgcaa tagcaagggg gggtaatgag
     1441 agaaagaaca aatatcatgc tagatatagg attaaagcaa tccttacaac ggctttcgaa
     1501 taaaaccaaa atgagtctaa gtgatcttat taaccagaga ttatcaggtt cactttcagt
     1561 tgaagaacgc attggcgaaa agcctgaatg gatgagaaac cttgatccct atctcgccaa
     1621 tcacttttca aatattgagt taccgctccc ttcaccatta aaagaatacc aagaaaaaat
     1681 ggatgagata gattcaatta ttaatgacat gatggttcta aaggaatatt tcagagctaa
     1741 gttttacgac gaaataaaat aggcacgcta tacggaggcg gagaattatg ttttttaaac
     1801 ggtatttaac ggagaaggaa atatacaagg cagcactccc aattgttagg cttcaaggga
     1861 gagttaacac ttcattacgt tttttatcca aatactcaga tgtaaacaaa aatggattaa
     1921 ttgcattgca tcttaattta ttacatttta tctcatacaa catacacggt gtagagtctc
     1981 tgaaaatcac tcaagcaggc ataaacaaag ctgtacaaca aagccctcac gcagatgaaa
     2041 taaaaaatat tgcgcaggca ataggacgca aaggacctga gtacatcgaa ggattaatta
     2101 gaaacccatg ggggaagaaa aatgataatc cactaatatt tataatgagc attgaagaaa
     2161 gtataaaaga aacccttaac tcaggttata tgttatatag agtagctccc ataactgagg
     2221 gtgaagcagc tgctttcatg gatgttctta aacaattatt attatttgag gataattttc
     2281 gcctttttgg tagagacttg gtttataaaa tagaaaaatt aaaaatcaga taacaaagta
     2341 tggtgaacga aaaatgaaag agaggatagt gaaatgtgta ataccaagat tctcaactat
     2401 gtattggtta attgcgttcc tagcatttat attccccggt cgatcttttg ctgacggtta
     2461 caccgcttac taccgatgta atggtactca ggaagcatct cttactgtta aagataacaa
     2521 actaagtatg gttgtaggtc tttctaagtt taataggcaa ctggatactt atagattcaa
     2581 agaaaaggga atcgaagtag taatgcagag agctgcttct gatgataact ctgaggccat
     2641 aatgtctttt attatgcctg acgttaacaa tatgacctta ttagttaaaa ataacggcta
     2701 cattatcgtt aagacaacct gctggttaac taaaaccgac aaacagtaga acacgtagtt
     2761 agtcgagtat ttaccataat acctgttgtc ggcatcaaaa aagcccgcat agcgggcttc
     2821 agtcgtaaac ttcgcggcga tgtccaatcg tggcggcaag gataacaagc tcgtcatctc
     2881 ggatctcaca cagaacgcga taatctccca cccgatagcg ccagaactcg cccagctcac
     2941 ctttgagcgg cttccctgac tggcgaggat cggcagcaac tgcaatgcgt aaactcataa
     3001 aatccacaat ccgtcgtgcg ttctgtttgt ccatcttgcg taacgatttg agcgcccgat
     3061 cggaatagtt aatcgtccag accaagtgac ttcctcacgt cttcagacga atgcactgct
     3121 tcatcaccac ggcgaacgcg tgcagcagtt tctgctgaaa gataataatc ctccaggtct
     3181 tccagatgcg ccagtattgc ttcccgcgca taaaacgtct ttgttctgcc ggtttgcttc
     3241 gccagcgagt ccagacggga ctcaatctca tcagataacc ggatagcaag catataaacc
     3301 tcccgaacac catttgctat acatgtatat caacagtacc acaatcaacc taaaagaaca
     3361 aaaaataatc ttgttagccc ctcttgataa tccgtaacgc ggtcaatata tctagcttga
     3421 gggctttcct cttaactatt gaaaggataa cgattatggg tcttaagccg ggtccgaaac
     3481 gaattgccga ttcaacaggg gaacctgata aacgccagcg tgacaataaa aaaacgccgg
     3541 gaaatactga caaattgaaa cccagcaaat cttcaaaaaa ataagtcagt agttaaaagc
     3601 cctccggggc ttttttgtat ctgccgttca cgctgattaa acataacacc cgttgcccgc
     3661 accgccccgg acgggttcgc cgcgaacggg gtggcagtca ggtaaaaccg gcaacaaacc
     3721 acccgataaa ccgccccgtt cgcgacagat aaattttacc tggctgcata gccatgcagc
     3781 caggtgaatg acatccggtt tcagcgtaaa tgcccgatga gaaccctgtt ttcctgcatt
     3841 tcagcccagc gacggaccgt catagcttcg ctgccgggaa cgcgcttccg tcagttcctt
     3901 ttcgtgctgc cgttgctcgt gcgcccgttc tgcggccacg gccatctcat gacgctgcac
     3961 taccggctca aacgctgcag ttgcccgctc aagcgttcca cctgctgact caagcgcgtc
     4021 acgcgctcgc tcagcgccgc gttctcccgt tgcataagcc ctgacatttt ccgccattcc
     4081 ccgaaggcgc tctcccactc gtccagcctt ttcgagtagt cctgctgtag ctgctctaat
     4141 gcgctcagca actgtttttc cagctcggtc atcggcactt actccttcac cagtctcagc
     4201 gcgttctgct tcgaccgccc gttcccgacc gtccagctct gcgtcctgtc tacggccgtc
     4261 cccttcggca gcaccagaaa acgtccgttg ctgtcttcca ggtacgtcac gccccaggtc
     4321 ttcgcgttca gcttcgacag cgctgcgttc tgccggtcga tttcggccag atttgacgct
     4381 atcaagctcc cctgatacca caacaccccg ctcaggctgg cgaacagcag tcctgacacc
     4441 atcgtgatgg tcagccatgt ccgcccgacc atacgcatca cgctcaggcg gttcgactgc
     4501 atggccgcgc tcataccctg ctcgtgggcg cggatggcgg cgctgattct cttcccgctt
     4561 tcgctcagtt ccgctctgac agacttctcg tgctcgctga atgcggcttt cagcatttcg
     4621 ccggtactct gctgctgcgc tttcgatttc tgctctaagt ccttcgccag cgttaaaaga
     4681 ctgttcatag atggctccct taagtcggat gtttcgccct ccgtccgggt cggcaatgct
     4741 gatgctgctc tttgtggtgc gcacgacctc aaaacctgcg ctttccagcg cctcagtgac
     4801 gtcctgacgc gttttaagct cccctgacga ggcgagggcc agtaagcccc gcgtaatagc
     4861 ctctgcggct tcctgcttcg ttttgggtaa cgaagatggc gtcaccagcg cgcgccggtt
     4921 ctccggtgcg ttcgggtcgt gcagccccag tcgcccgttc accacggtct gccacgcatc
     4981 gatgcgcgga cggtctgccc ggtcgtagta cggctggaga cgcctgccgg tcagcagttc
     5041 cgtgttcggt accagaaaat tcagttccag ccgccccttg tcccggtgtt caacccacag
     5101 cacgctgtac tggtctttat cgagtccggg catcagaacc cgctcgaagc tcgccatcag
     5161 cttttcacgc tggccgggcg gtaaatcctg ttcagcaaaa gacaggacgc cggaggtgta
     5221 ctttttggcg tagggcgagg cgtcgataag ctcccggacc tcctccggct ttccctgcag
     5281 gacgatggcc ccgtcgcgct ggcggtcttt ccccagcaga taatccaccg gcccggcacc
     5341 gccgcctcgc cctcgcggat gaaacttaac gatcatcgtc tgcccccttt tccagcacgg
     5401 catgccgcag ccgctcgagt tcggcatcaa tggccatcag cgcggccacc acctgaaccc
     5461 ggtcagcgcc tgaccactgg ccgccgttaa ttttccgggc gatctggttg aggttgttcc
     5521 ccatcccggc aagctggcgc agcaaaaccg gatcgatgga cggcagccgc aacgaccgcg
     5581 ccgggcgcgt atcgag
//
