LOCUS       NZ_CP147153            10060 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain SC173 plasmid p2, complete sequence.
ACCESSION   NZ_CP147153
VERSION     NZ_CP147153.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40144601
            Assembly: GCF_037157695.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Wang,D.
  TITLE     Klebsiella pneumoniae strains whole genome sequences
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Wang,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2024) School of Life Sciences, Taizhou University,
            1139 Shifudadao Avenue, Taizhou, Zhejiang 318000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.15.3; Canu v. 2.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina NovaSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037157695.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 18:40:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,476
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 45 of 129
            Pseudo Genes (incomplete)         :: 85 of 129
            Pseudo Genes (internal stop)      :: 15 of 129
            Pseudo Genes (multiple problems)  :: 16 of 129
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147153.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SC173"
                     /isolation_source="biliary fluid drainage"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2"
                     /geo_loc_name="China: Taizhou"
                     /lat_lon="28.40 N 121.26 E"
                     /collection_date="2020-09-04"
                     /collected_by="Dongguo Wang"
     gene            116..361
                     /locus_tag="WBS27_RS27870"
     CDS             116..361
                     /locus_tag="WBS27_RS27870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            635..1006
                     /gene="tolA"
                     /locus_tag="WBS27_RS27875"
     CDS             635..1006
                     /gene="tolA"
                     /locus_tag="WBS27_RS27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            1003..1368
                     /locus_tag="WBS27_RS27880"
     CDS             1003..1368
                     /locus_tag="WBS27_RS27880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            1543..1821
                     /locus_tag="WBS27_RS27885"
     CDS             1543..1821
                     /locus_tag="WBS27_RS27885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            1809..2123
                     /locus_tag="WBS27_RS27890"
     CDS             1809..2123
                     /locus_tag="WBS27_RS27890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2287..2715
                     /locus_tag="WBS27_RS27895"
     CDS             2287..2715
                     /locus_tag="WBS27_RS27895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            2741..2920
                     /locus_tag="WBS27_RS27900"
     CDS             2741..2920
                     /locus_tag="WBS27_RS27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2947..3477)
                     /locus_tag="WBS27_RS27905"
     CDS             complement(2947..3477)
                     /locus_tag="WBS27_RS27905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3484..4215)
                     /gene="mobC"
                     /locus_tag="WBS27_RS27910"
     CDS             complement(3484..4215)
                     /gene="mobC"
                     /locus_tag="WBS27_RS27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(4215..6179)
                     /locus_tag="WBS27_RS27915"
     CDS             complement(4215..6179)
                     /locus_tag="WBS27_RS27915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            7300..7449
                     /locus_tag="WBS27_RS27920"
     CDS             7300..7449
                     /locus_tag="WBS27_RS27920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_319922287.1"
                     /translation="MGCKQELPVQPDCCALSGNCSLESNPGKHAKTPLAAAIGNWEFAE
                     DLFS"
                     /name="replication initiation protein"
     gene            complement(7663..7812)
                     /locus_tag="WBS27_RS27925"
     CDS             complement(7663..7812)
                     /locus_tag="WBS27_RS27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(7897..8154)
                     /locus_tag="WBS27_RS27930"
     CDS             complement(7897..8154)
                     /locus_tag="WBS27_RS27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(8164..9849)
                     /locus_tag="WBS27_RS27935"
     CDS             complement(8164..9849)
                     /locus_tag="WBS27_RS27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             3164..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          6982..7560
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.11"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3488..4215)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 agacattcat cacacttaca tttcagatgg tatagacaca tctgaaagct aactggcatg
       61 aggagaggac ctgcggttaa actgttcggg aaacctgaga cgcccggagg ccactatgac
      121 gcacctgaaa ctgactcccg ctgaagaaaa gcgcctggcg gacgcctttg tcgctcaggc
      181 actggccatg ggcctgaaag ggaacaaaac gctgccaaag gcggtaaaac aacaggtgcg
      241 ggaaagggtg cttcgtgcca ttcaggacga acgtgaagcc aggcgggcgc aggaaatgcg
      301 tcgggagaaa tacgaggcta cggaaaccac cttcacctgg cagccacagc gccgccggta
      361 aggggtattt accataacgt cgcattgccc gcaccgctca gacggtctga gggcgtttcc
      421 ggcggctacg ggataaaaca ggcaacaaac cacccggaaa accgccggaa acgcgcccgg
      481 taaattgtaa cccgctgcat gcctatgcac gcaactgcat ggcgggccgt ttggtgtaaa
      541 aacgatacga taagccgtgt ttatgtaaat gaacgtggcc gtaaagagtg acaaatatac
      601 atgtaacatt ttttactgac aggtcatcaa cccgatgcgt ttaataccct ttatcctgct
      661 ggcattgtgc agccccttac tcctgacggc ctgtaagcat tccaccaccc agccgccgaa
      721 aatttatact ctggataagt catctaaaat cagtagctac gctgcggcca tcaagaccgc
      781 tgttgaaatg cagatgtttg aaccagaggc ctatgcggga aaaatgtgca ccgtgcggat
      841 atccatacaa cgtgatggca ctctgaacag cgccattgct gaaggaggag atccgaaact
      901 ctgtaaagca gcaatatcag ccgttaccca cgccaaaatt ccgccggcac cggatgaaaa
      961 tacctggcaa atattcaaaa acgcccctct ggatttcaga ccatgaaagc ttttactctg
     1021 acagtgctgg cggccatgtt cctcactccg gtacatgcga taccactaaa acccgtaacc
     1081 acactgcagc caacctaccc ggtacaggcc gcagcaaaac gcatcgaagg tcaggtgacc
     1141 gccacgtttg atattctgga cgatggacga gtggacaacg tgtcgcttac cggctgtcac
     1201 cttttttttc gggaaacgct gacggcaatg aggcactggc tatatcccgc agggcacccg
     1261 gcaatgcgac aggttataaa ttttgaattc agtctgaaaa atgacggctt acaggatccg
     1321 cgaccagtca ccacccaaaa gctcaatgat gatggttgcc gccagtaaca ggtcctttaa
     1381 cccgcaggct gcaatagtcc tggatgatga gtgagcctgt agcagccctg attagtggaa
     1441 gcgaagctga aaaatgggca gcaaaagccg tatttctggc aaatagcgcc gccacggatt
     1501 aaagtagtca tgtgtaatac atatgactac aaaggggctt ttatgtcatc agcaacatcc
     1561 atcaggctcg atgaagaact taaggaccgg ctgaaaacgc tggccgatga ccggcaccgc
     1621 tcagcgcacg cgctaatgct ggaggcaatc accgaataca tcgaccggga agagaagcgc
     1681 agccagtacc tgcgggacgg acaggccgca tggcagcact atcaggaaac gggtctgcac
     1741 cttactgcgg aagaagcgga agcctggata agtacctggg gaactgagaa cgagcaggat
     1801 gctccgccat gccacaggta acgatttccg cgctggcaca gcgcgattta caacgccttc
     1861 aggactttct caaaaccaaa aatcggctgg cagccagaaa aggaggtgag gtgatcgtcc
     1921 gggctatcca gcaactgaaa acattgccag acattggccg cccggtgccg tttctgccgc
     1981 tggaatataa ggaactggtg atcgggtttg gcgacagtgg ctatgtgatg ctctaccgcc
     2041 acgaccggga aatggaccag attgtgatcg tcaccgtcag gcatcagaaa gaatccgggt
     2101 atccgggggc agacagcctc taataaccga tgacgggggc gcaattcatt gcgtggccgt
     2161 cgccggtttg acaagcgcag cgcgtcagaa aaccacatcc tgaacggaaa aacgggtaaa
     2221 attcagcgat acatttaccg tgagaaatta cacacctttc acacccgata aaatcagggg
     2281 tatacagtgg cgaaaacatt gctcaacctt tcacaggcag cacaagccgc gggcattacc
     2341 agaagaacgc tatacaatca tgtaaaacag ggaaaggtta ccgtgtcacg tgacgggaaa
     2401 aacaaccctg tcgtggatgt gtcagaactg atccgtgtat acggaaatgt gaatatccct
     2461 gagaaacaaa ttcccggcat ttcacaccgt gaaaatacac aaaaaaattt cccacaggaa
     2521 cagcttctgg caatgcaaaa ggaactggcc gatctccggc aggctgtcac cctgatgctg
     2581 gaagataaaa catcccggga agaagaacgc cgccagcacg atgatgagcg ccggaaactc
     2641 caggccgaag tggacagatt gacaacagaa ctgacacaga agaaaaaacg cttctggtcc
     2701 ggctggttta gctgagaact taccgaaggg aatggagaac atgacgaagc aggaaaaaac
     2761 tgccctcaat atggctaaat tcattaagga tcagtcgctg ctgttgctgg aaaagctgaa
     2821 cgagctggac atggataccc cggccgacat gtgcgaacgg cttcacgagg atgcggaaac
     2881 actctggcag gtgatgcggg ataagctcgg ggaagaataa cagcaccggc catgccggtg
     2941 ctggcgttat tttttgccga acggccacca ccggcgggcg ggtttgtcct ccggcagttc
     3001 aggcaccggc acgttaaaat agcggctgtt atattcccgc atcagctccg ctttcagctt
     3061 ctcgttcacc gcgctttccc cgtgccgctc gctgaccacc tcaatcatcg cgttaatctg
     3121 ctccggtgag cacttcccgg catcaaccac ctgcacaccg ttctcgatgg cgaaccgctc
     3181 cacctttcga tagaggtaat cacacagctt cattcctgtg ggtttgtccc gttcccattg
     3241 cggattttca ccggtgaaaa agtacgtggc tttttcgtcg ctgttgagcc gctcgtcata
     3301 catcccgatg gatttcatca tcgccagatg ctgggcccgg tcgacaaaac agaaaacata
     3361 cgccccggtc tgccaggcga gcaccaccat gtcttccaga tgctcgctgg ttggcataat
     3421 gcagcgctcg ccgtctttgt cttccacgtc ctcaataaat accatgtaca gcgccataat
     3481 ccctcactcc gttgtcataa atttaaagca cgcgaacagc ctctcccggt ccagctccac
     3541 cgttttgcgg gcctcgccgg tctgtggatt cacgatttca tccaccgcca gaatcgcccg
     3601 ctgtacccgg acaagcgtcg gccggtcagg gcagatgtac cagatttcat cgatgccgtt
     3661 cgcccggcgg ttgaacagat acccggacac cacttcctga taccgcctgc ggctcttgac
     3721 ggtgcgctct acctcaaatg ccaccgttct gccgtccagc tccgccagcg cgtccggcac
     3781 tttcatccct ttcagggcca tacggtgcag gtaccgccag ccggtggccc cccggccctc
     3841 catggagagg cgcagggact gcacggccag ccggtgcggc agctgcgccg ccgacaccct
     3901 tgacggctga aacggaataa cgtccgtcag cggctcgtcc tcgtcgaacg agaacgccac
     3961 cccgtgcggc gtcagccccc acacgctgac ctgacggccg tcaaccgggt atttcgcccg
     4021 gaccagcagc cctttcgcca ccagactgtc caggaagcgg taaaccggct gtaccgccgc
     4081 aatccccagc cgtcgcgcaa ccagctccgc cgtggaacac agctcttccc gcagccagcg
     4141 cagggtcagc cgtgttttct ccgcgttccg cgcctgccgc ttcgcgtggc tgacgttgta
     4201 ctgctccagt gtcatcagta aagccccgca aaatcatcat ccgcttttgg tccgccgccg
     4261 tcggccggag gcaggtactc cgccagcggg tccgttttcg ccagcaccgg gaaggttttc
     4321 agcgaaattt cccggcgctc aacccgcagc gggctgctga acgccagccg cgccacgccc
     4381 agcccggtca tcaccgcgaa cccgtcaggc aggtgttgca gggtgttcac gtcaaacagc
     4441 ggccgcgcct ccttctggat gaagctgtcg ccgctgatgt gctccacgtt ccccgcctcc
     4501 cggctggccc ggcggcgctc cacgtccacc cggatctccc cggtctggcc cgccgcccac
     4561 tgcgcggact cctggctggc cgcgcgatag aaccagcgga tcggcgtgtt atccagtacc
     4621 gtggttttca caaaatcagc gggcagatcc tgcccgcact gaccgaagtc gcccagcgac
     4681 tggtgcgcga gcagcaggtt gcagtcccgg ctgcgcagcg tccccagcgc gttcagcacg
     4741 tacttcgaca gcagatattt aatctcatcg agcatgatgc tcgcgtgcgg ccacctccgg
     4801 aactcatccc gcgcaatgat gatttgcgcg cagcgggcaa acagcatctt ctgcacccgg
     4861 atcaccgcct catcgtccat cgagccgatg acgtacaggc agccgccgcc gttgattatc
     4921 cccgccacat cgataccgct gtccgtctgc aggacgctca ggtcggccac gttctccagc
     4981 ttggtgatca gccccttcac gtcctttttc agggcctccg gcaacgcgta cgccgccgcc
     5041 agcacctgct gaatgtttgc cccctgcggg aactgctccg caatcagctt cgccgctttc
     5101 tgctcgctga tgcggtagtg gtccgccacg tcgccggtct cactcaggtt aaacccggca
     5161 accagcagat tcttcagggc gtactggtcg agatcgcgga acagattcag ctgcgccggt
     5221 ttccccggcc gcagatccag cagggtgaag tccgggcagt gcgcccggaa gacgtgcggc
     5281 gcccacgcgt cccctttggg gtcgaagtac accacagcgt ccccgaaatc gcgtacgcag
     5341 cgaatgagcg cgttggtggc catcgcgctc ttgccgctcc cgggcaggcc gaggatctga
     5401 atattggtct tgcgccaggt atcacgaggg accagcaccg gctggccctg ctcatcctta
     5461 cccagaaaca ccgcctgctg tttccgggcc tgctccgtgg aaaaatcggc cagcgcgtta
     5521 accggtaccg gctccccgaa atgcaggtgg cggacgtctg tcatttctcc ggtccgctgg
     5581 ctgctgcgcc ggctctcctg attcagtttc gcgttcaccg cctcgtcagc ggccgcaaaa
     5641 tgaccatcaa gcagcaggaa cgccagcagc cgcgccagaa ggacgccggc gacaagccct
     5701 ccggcacacc aggctatccc gaacaggtta gccggctgca gcaggtaata atctgcgttc
     5761 gcatagaggt ccgggcgcag gacggtttct ccccagctct cgataaacac ggcgacaacc
     5821 agccccccca gaagggccag accggcagcg ccggtcagcc agtacgcgta gttccagaca
     5881 tgtcgccgtg ccctgaccat ccgcccgggt gaatccagcg aggccagcac cgaaaatggc
     5941 acagtccggt tattatcgcg cttatagcga tcggcatact cccgccgcgc cttatggctc
     6001 agccccacaa aacgcattcg gccggcccag agttcagcca gaccgaaaaa caggccgata
     6061 accagaaaaa aaccggccag actgtaatac tcccggtaca gccagctgac ccacgggtaa
     6121 atctgccggg ccagctccgg gatatgccac tgccaggcaa gcgaatccgt attaaacatg
     6181 caatctctct ccttcagacg acgggtttac cggcgtgatg cgcagccccc gctgcacaag
     6241 ccgcggacat gccccaaggc atggcggcgg cggtcacgtc cgtggtgatt gcgttcagct
     6301 ttcaccgcgg aggccagcct ctttccgccc catgcggaaa aggcgcagcc ccggaggaac
     6361 aacgagcacg ccccagcgtg tgagaggaaa aggcggcacc ccagtgcggt cttttccggt
     6421 gttccggccc ccgtcagagt tcgccatagc gagcggctga ccacccgcaa aatcaggagc
     6481 agctttgctg cttcgtcagg tgcgtccagc accgtggatt tttgtgggtg gtcaggcgtg
     6541 agcctgacgg gggccgggtg aatttctggc gtggccggcg tccagccgga cagagccaga
     6601 aagaggggaa aagaccgtgc gtcggtggcg tccagccagc gtaagcaagg tcttttcccc
     6661 tgaagtcagc cacgccccag cggggatgac ttgcatttct ccgcaaggag aaaaaagcaa
     6721 cagagatagg ttaacgtaaa atgttcagcg aaaaaccgac atggttcacc tatcctgata
     6781 attgatcgtc agggaataga aagacttaat caggggacaa tatcccacat cagcgcgtcc
     6841 ggtgaccgtc ttgcaggtgc cggggaaatt gcgcctcctc gctcactcgg tcgctacgct
     6901 ccgggcgtga gactgcggcg ggcgctgcgg acacatacaa agttacccac agattccgtg
     6961 gataagcagg ggactaacat gtgaggcaaa acggcagggc cgcgccggtg gcgtttttcc
     7021 ataggctccg ccctcctgcc agagttcaca taaacagacg ctttttcggt gcatctgtgg
     7081 gagccgtgag gctcaaccat gaatctgaca gtacgggcga aacccgacag gacttaaaga
     7141 tccccaccgt ttccggtagg tcgctccctc ttgcgctctc ctgttccgac cctgccgttt
     7201 accggatacc tgtccgccgt tctcccttgc gggaagtgtg gcgctttctc atagctcaca
     7261 cactggtatc tcggctcggt gtaggtcgtt cgctccaagc tgggctgtaa gcaagaactc
     7321 cccgttcagc ccgactgctg cgccttatcc ggtaactgtt cacttgagtc caacccggga
     7381 aagcacgcaa aaacgccact ggcagcagcc attggtaact gggagttcgc agaggatttg
     7441 tttagctaaa cacgcggttg ctcttgaagt gtgcgccaaa gtccggctac actggaagga
     7501 cagatttggt tgctgtgctc tgcgaaagcc agttacccgg ttaagcagtt ccccaactga
     7561 cttaaccttc gatcaaacca cctccccagg tggttttttc gtttacaggg caaaagatta
     7621 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctactgaa ccgcgatccc
     7681 cgtcagttca gaggaggacg atggtgccac cgttccgccc tgaacatccc ggatataatt
     7741 tgcctgacat gcggccagaa gaaagccagc aacaatcaat ataaaaagaa aaatagtctt
     7801 tacctttttc atatactttc cctgtattta aagactccgg cattcatccc tgaacaccgg
     7861 aagtcagtat ttcaaatcat ccttgataat tttttgttac cagttaccgt cccgataatc
     7921 aaaagctata taacaattat ccacatcagg ttttatttga tttttaaaaa atggctgtag
     7981 taaaggaacc cattctttct tcaaatcaaa ctcaccatta ttaatattat cctttaaagg
     8041 cattcccaag ctttcaatga cagaaccatc gtcaccaaag tctttagaat actcttcccc
     8101 tttaaactct tcagttttct tatcaaacca tgtcaaacgt aatttaagcc ccatatttac
     8161 ctctcaaaga tattttttaa tgttccgttt tggatctggc cctttaacct gcttacccgt
     8221 tttgggatca aatgcgccta ggtgttcgcc atcactggca cgataccctt caagctcacc
     8281 gtgctgggaa tcccattcat aaattttacg ttttttatcg ccataccatc gggcccgctt
     8341 accaccacca ccttgtttag gtgttttagg atcacctcgc tttaactctc caagtccctt
     8401 tatgtcttct gttttaggta ctggatgata atcatggccg tagtctttag tccccttgcg
     8461 aggcttgttc ttttcctcat ttaacttatt ttctgcatcc ttcgcttttt tctccttttg
     8521 tttccggctc tccatcgccc cggtaagtgc agccttatct ctggataagg attcattcat
     8581 tgcgtttaaa gatgcctgag ccgcttttaa tttctcattc tgattctgaa tatccttatc
     8641 aagatagcga acctgatcct gccacccgga gccatattca tgaggcgggt tataagtcac
     8701 aaaatcatct ttatttgcct ctgcctcttt taccttttta tcggcttcct gaacagcagc
     8761 ccttgcaccg gggagcgcct tctctgtcga tgcaatgcgg ctgtttaagg tcgcaatatt
     8821 tttatcttcg gcatccagct cagctttcgc tttatcatag tctcttttca gcccctcttc
     8881 cggatgagcg gcgtcccatg cctgctggcg acgcttttct tcctcctgac gctgttttac
     8941 ctgtgccggg gtaagaacat ctgtcaccga cacataaacc ggcttctgtc cggtctcttt
     9001 cgggaaacga ataacggcct cacgggagtt accaccggct gtaaaaccag ccggacgtga
     9061 atcacctttt tcagcaacaa tgccttttgg cggggctttc gctgtcggaa cacctttcgg
     9121 tacgctcacc tgcagagacg ggagacccgg aatcgacaca ctgaataccc ccggacgttt
     9181 tgtcggtttc gcatccacca cagggacact cattggccga cctgcgacaa ccgcaatatg
     9241 ctgccgctca tccttcacca catccacaac ccgttgtctg acacgaaccg tcgcctggtc
     9301 cagtggtaaa gtacttgccg gagtctccgt caccgcatcg gccggtaatg acgtcataat
     9361 ttttgacatc attttcggat catcttttgc aatctcagaa ggcagcacac cgtagatcgc
     9421 aatcccccac agaccaaact taaacggccc cttcagggca gccagcacat cagcaacggc
     9481 ggccgacaac gcatcgccgg aaactgataa agccagtcct tcagcccccg gagtagccag
     9541 ggcaggcaga ccgaaggcca tcgcagtggc cgaagactgt ccgccaccag agctaccgct
     9601 atgattgccg ctgttaccac cattaccacc gggtttactc ccgccgttac caaactcccc
     9661 cggattatag ttatgaatat ctccattcgg cgtatgcgtc gtaccccagc ccggcccgct
     9721 gtttggatcc gtaccaccac ctgatgaagg gccaccttta ccagacgtac cgttcacatt
     9781 cccactggcc tgaccaccat tatgtcccag acctgaatta cccggacctc gaccgtctcc
     9841 accactcata ccgttttcct ctttgacatt taaaacaata aattaaagcc aacatactgt
     9901 acatatacac agtataatta ttaccttact cctcccgttt tcaggtgtca atatatgaga
     9961 ttagttctca tcttcaggca caaaaaagcg cccggagcca cctgtaagca gatcgcgtct
    10021 tttcccgaaa atttctcatg gcaacaaatt ttattattta
//
