LOCUS       NZ_CP147138             1488 bp    DNA     circular CON 22-APR-2024
DEFINITION  Escherichia coli strain KFu015 plasmid pKFu015_8, complete sequence.
ACCESSION   NZ_CP147138
VERSION     NZ_CP147138.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39992786
            Assembly: GCF_038396395.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Gomi,R.
  TITLE     Genomic analysis of environmental Escherichia coli carrying
            transferable quinolone resistance determinants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Gomi,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2024) Department of environmental engineering,
            Kyoto University, KyotoDaigakuKatsura, Nishikyo-ku, Kyoto-city
            615-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038396395.1-RS_2024_04_20
            Annotation Date                   :: 04/20/2024 18:35:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,106
            CDSs (total)                      :: 4,987
            Genes (coding)                    :: 4,790
            CDSs (with protein)               :: 4,790
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 197
            CDSs (without protein)            :: 197
            Pseudo Genes (ambiguous residues) :: 0 of 197
            Pseudo Genes (frameshifted)       :: 59 of 197
            Pseudo Genes (incomplete)         :: 133 of 197
            Pseudo Genes (internal stop)      :: 38 of 197
            Pseudo Genes (multiple problems)  :: 35 of 197
            Pseudo Genes (short protein)      :: 5 of 197
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147138.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="KFu015"
                     /isolation_source="river water"
                     /db_xref="taxon:562"
                     /plasmid="pKFu015_8"
                     /geo_loc_name="Japan"
                     /collection_date="2011"
                     /collected_by="Ryota Gomi"
     gene            complement(join(997..1488,1..33))
                     /locus_tag="WAZ88_RS25530"
                     /old_locus_tag="WAZ88_25530"
     CDS             complement(join(997..1488,1..33))
                     /locus_tag="WAZ88_RS25530"
                     /old_locus_tag="WAZ88_25530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634347.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="WP_113224162.1"
                     /translation="MLVNTEGQANQRLKELEKIKNKASVEHEEAKKNRNFTQMYPKGWQ
                     RVRELLKDKQGISAIQLYSFLAEHIDPTCGAVVCDQQFLAEKMDVSTRTIRRWLTYLEG
                     KNALVRIPVAGKVCAYALDPHEVWKGYDTSKEYAAFVTKTLVNKDGEIQRRIMAMFSGT
                     ESGQEQKRSDS"
                     /name="DeoR family transcriptional regulator"
ORIGIN
        1 ttgattggcc tgtccttcag tgttaaccaa cattcgagat ctcccatctt gatttcgtga
       61 cctcatgata ggtcattttt gtccgattgt aggccaaaac gcgtcctacg tgaagacatg
      121 agcggacatc cagggtgtcc ggatgagcgg acacgcaggg tgtccgcata gaaaaaataa
      181 aattaaataa aatcaatttg ttattaattt gcccttttct attctttgaa aacctaaagg
      241 caacaccaaa attcaaaacc acgaaagtag ctccctccct tcggtcgggc atttttgggg
      301 tcaaggttcg cgttagcgac cgcgaagcgg cttgaccttt acgccaaaaa ttcccgttca
      361 cacttgtgag tggtggttgg tttcacgaag tgaggccaac cacctgcaaa agctattttt
      421 taaagttcat gacgatcacg attacgtcga taataatttt ggcgatttcc agtgtaagtt
      481 tcatttaaag ccctttaatg attttgttgt ggttccggca gttgattgtt tatggctaca
      541 aaagtcgaga aacagtgcca gaggtacaaa tgccgcaaca tcgaatcgaa gttagattta
      601 cacgtatcca ttccattgat aatgctgatg gagttcagtg ctattcacct ttgtattttg
      661 ctgtcacgac gccaacaggc ataaggtttg aagttgtgtg atgagtcata caacgaccga
      721 taagcttttt tggttttgtt tttggggttt cggggcgcag ccctgaacca gtcacgacgc
      781 gctagcggtg tgtatactgg cttacctgta ttttggcatc gcagatcgga cagtttcgaa
      841 gaaactacta agtgcgcatt tgaagctttt gaattcgtat tggatgtgtt tgtactaggg
      901 gatagaggct caagaaatac caaacccgct tatcaggggg atggctcgca gtggcgaacc
      961 atcccccata tcaaggatgg gggtcacttc aagccgtcaa gagtcgcttc gcttttgctc
     1021 ttgaccgctt tccgttcctg aaaacatggc catgatacga cgttgaattt caccatcttt
     1081 attgaccaag gttttagtta cgaaagcagc atattcctta gatgtatcat aacctttcca
     1141 cacctcgtga gggtctaaag catatgcgca aaccttgcca gcaactggta ttctaactaa
     1201 cgcatttttt ccttcgaggt acgtcaacca acgacgaata gttcttgtcg agacgtccat
     1261 tttttccgct aagaattgct ggtcacaaac tacagctccg catgttggat ctatatgttc
     1321 agctaaaaaa gagtaaagct ggatagcgga aataccctgc ttgtctttca gaagctctct
     1381 aacgcgttgc cagccctttg gatacatctg ggtgaagttt ctgttcttct tcgcttcctc
     1441 atgctctaca gacgccttat ttttgatttt ttctaattct ttgagcct
//
