LOCUS       NZ_CP147137             3560 bp    DNA     circular CON 22-APR-2024
DEFINITION  Escherichia coli strain KFu015 plasmid pKFu015_7, complete sequence.
ACCESSION   NZ_CP147137
VERSION     NZ_CP147137.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39992786
            Assembly: GCF_038396395.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Gomi,R.
  TITLE     Genomic analysis of environmental Escherichia coli carrying
            transferable quinolone resistance determinants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Gomi,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2024) Department of environmental engineering,
            Kyoto University, KyotoDaigakuKatsura, Nishikyo-ku, Kyoto-city
            615-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038396395.1-RS_2024_04_20
            Annotation Date                   :: 04/20/2024 18:35:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,106
            CDSs (total)                      :: 4,987
            Genes (coding)                    :: 4,790
            CDSs (with protein)               :: 4,790
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 197
            CDSs (without protein)            :: 197
            Pseudo Genes (ambiguous residues) :: 0 of 197
            Pseudo Genes (frameshifted)       :: 59 of 197
            Pseudo Genes (incomplete)         :: 133 of 197
            Pseudo Genes (internal stop)      :: 38 of 197
            Pseudo Genes (multiple problems)  :: 35 of 197
            Pseudo Genes (short protein)      :: 5 of 197
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147137.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="KFu015"
                     /isolation_source="river water"
                     /db_xref="taxon:562"
                     /plasmid="pKFu015_7"
                     /geo_loc_name="Japan"
                     /collection_date="2011"
                     /collected_by="Ryota Gomi"
     gene            complement(323..853)
                     /locus_tag="WAZ88_RS25515"
                     /old_locus_tag="WAZ88_25515"
     CDS             complement(323..853)
                     /locus_tag="WAZ88_RS25515"
                     /old_locus_tag="WAZ88_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012634455.1"
                     /translation="MASTEEKLLTLLLSAEHLKEAAKEQQKAVADSLAVARETLKGYRE
                     ISDSVAQQVRDEVRQEVQKMDVAGLIGERISADFQRLEKSVGNMKINADALDKEISIIR
                     SNIIAEYNTLRGYSWKWFAGVAVAFVVLIFATSWFVKTELQDNYNASAAVYQKVADLEK
                     LLTTEQKPEKKKN"
                     /name="hypothetical protein"
     gene            complement(856..1791)
                     /locus_tag="WAZ88_RS25520"
                     /old_locus_tag="WAZ88_25520"
     CDS             complement(856..1791)
                     /locus_tag="WAZ88_RS25520"
                     /old_locus_tag="WAZ88_25520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_045626452.1"
                     /translation="MATYHLSVKFGGKGKALAHASYITREDKFSQRQDLEHTEHGNMPE
                     WARDEPAHFWQAADAFERANGSTYREIEIALPRELNEAQRLALVRDFVKQETGDKHAWT
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERSPEQYFRRYNAKFPARGGARKDSGHNL
                     TPTEQKQALKALRERWERTHNAHMKRYGFEQGFIDSRSLKTQGIDRKPEQHLGFEAAGR
                     LDDSQRQDIKDSRTFSVVDVPEQAKHIDHSDRSWMNASGIEVNRKELVRSLTHDISQQY
                     GREMAARVTKQGNVEIERVKSLSRGFSISR"
                     /name="MobA/MobL family protein"
     gene            1958..2344
                     /locus_tag="WAZ88_RS25525"
                     /old_locus_tag="WAZ88_25525"
     CDS             1958..2344
                     /locus_tag="WAZ88_RS25525"
                     /old_locus_tag="WAZ88_25525"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045282373.1"
                     /translation="MRISNVEWLKKRIAFIRKLGEKTARQRQIIDLLDDEASLSETERR
                     LLHVLATAEKNALQERDAAQKKAVQARISGQKNRRERNHRLFLAAGLMIDAGLVDTKTG
                     ELNFDHEKLLTALKRVRYDLETSA"
                     /name="hypothetical protein"
     Typing          complement(860..1791)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.177"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttcactcac gacggtacac aacggcaggc acgtcaaagc caccggattt accataacat
       61 ccgttacgcg caccgctgaa acgcgctcag ggcggtttta cgtcctgagc cataaaaacc
      121 cccggcttct caccgaaaac cgctcagaat cgcataccgt gattttcagc cccatgcata
      181 accatgcacc tgactgactg gcgagaactt ccggcctgaa ttcaggccgg aagtctcaaa
      241 agacgcccca aactcggctg aaaacggtga acgggtgcgc aatttattgt gcgaacgtga
      301 ggcgttggac aagcgcagcg cgtcagttct ttttcttttc cggtttctgt tccgttgtca
      361 gcagcttttc aaggtctgcc actttctgat agacggcagc agacgcgttg taattgtcct
      421 gtagttccgt tttcacaaac cagcttgtgg caaatatcag tacaacaaag gcaaccgcaa
      481 ccccagcaaa ccacttccag ctgtaacccc gcagcgtgtt gtactccgcg atgatgttac
      541 tgcggataat ggaaatttcc ttgtccagcg cgtccgcatt gattttcatg ttcccgacgg
      601 atttttccag acgctgaaaa tcagcgctga tacgctcgcc aatcagcccc gcaacatcca
      661 ttttctgcac ttcctgccgg acttcatccc ttacctgctg tgccacactg tccgatattt
      721 cccggtagcc tttcagggtt tcccttgcca ccgccagcga atccgccacg gctttctgct
      781 gctcttttgc ggcctctttc agatgctccg ccgacagcag gagtgtgaga agtttttctt
      841 cggtgctcgc catagttacc tgcttatgga aaatccgcgt gacagggatt tcacgcgctc
      901 tatttcaaca tttccctgtt ttgtgacccg tgcagccatc tcccgcccgt actgctgcga
      961 gatatcatgc gtcagagatc tgaccagttc cttccggttt acctcgatac cgctggcatt
     1021 catccagcta cggtcgctgt ggtcgatatg tttcgcctgt tcaggcacat ccacgactga
     1081 aaacgtccgg ctgtctttaa tatcctgcct ctggctgtcg tccagtcgtc cggctgcttc
     1141 aaaaccgaga tgctgttccg gtttccggtc gataccctgt gttttcagtg accggctatc
     1201 aataaaaccc tgctcaaagc catatcgttt catatgcgcg ttgtgcgtac gctcccagcg
     1261 ctcccgcagg gctttcagtg cctgtttttg ttctgtaggg gttaaattat gccctgagtc
     1321 cttgcgcgcg ccgccacgcg ccggaaattt ggcgttatag cgcctgaaat actgttcggg
     1381 actgcgctca ataccgtcat tgacgcgctg gctcatcatg atatgggcgt gcggctgttc
     1441 gccgccgtca atgctggctt tcggattgtg gatcgcaaac gtccatgcat gtttgtcgcc
     1501 cgtttcctgc ttcacaaaat cccggacaag cgccagccgc tgcgcttcat tcagctcgcg
     1561 gggtagagcg atctcaattt ccctgtacgt tgaaccgttg gcacgttcga acgcatccgc
     1621 agcctgccag aaatgggcgg gttcatctct cgcccattcc ggcatattgc cgtgttcggt
     1681 gtgctcgaga tcctggcgct ggctgaactt atcttcccgc gtaatgtaac tggcatgagc
     1741 aagggcttta cccttgccgc caaatttcac gctgagatga tacgttgcca tgttgtgtca
     1801 gcccccggca gggcgcactg tttctgcccg cagggagaaa catataagtg cgctttactc
     1861 taatttaaag aattatgagt aaagataagt catgcgactt ctgaccgcaa gttatatact
     1921 ttccccgtcg gaaatatcgg gatggggaag ttaaacgatg cggatcagta acgtcgaatg
     1981 gcttaaaaag cgcatagcct tcatcagaaa gctgggagaa aaaacggcac ggcaacggca
     2041 gattatcgat ctgctggatg atgaagcttc gctcagtgag actgaaagac gactgctcca
     2101 cgttctggca accgctgaaa aaaacgcgtt gcaggaacgg gatgccgcac agaaaaaggc
     2161 ggttcaggcg cggatttcag ggcagaaaaa ccgcagggaa cgcaatcaca gactgtttct
     2221 ggcggctggt ctgatgattg acgcgggact ggttgataca aaaacgggtg agttgaattt
     2281 tgaccacgaa aaattactga ctgcgctgaa acgggtacgc tacgatctgg aaacatcggc
     2341 gtgaacgtgc attctgcaag gccggagaaa tgccgcttcc tcgctcactg actcgcgtcg
     2401 ctcggtcgtt cggctgcggc gagcggtagg gattttcgga taggagcatg aattttcttg
     2461 ctccagatgt acaaaagccc cgtgacagaa tcacggggct tgagtactct aattcttgca
     2521 gatacctgcc aggatcagac gtcacaatca aatcctagca gggctggcta ggaatgtcac
     2581 taccagctta ataccattca acgacattaa acccatatct gcaccttgac tctcaggtct
     2641 acgccccgga ctccggcagg ataaacaggc aagcggtggg aacctgacca gctcgggaaa
     2701 gaagctggct ctacccggtc tcacgttaac caataacgta cctgataaag tgagagtgaa
     2761 ggcagcggtt gcggtgaagc gtgattgcca aggtctggcg caacacccac aaaatcgaag
     2821 cagcatacga acggcctgat gctggtcgct tcgctccccg ttttctggtg tacccaccac
     2881 gggggcatta ggctttcttt gccctaatat gggtttgcga ctctcaaatc cgggttatag
     2941 aaggattcac tgtgatgatc cagcgtccga gatttgcatc gaattctgat ttagtgctga
     3001 acgaatttgt agtgtgagtt catcacactg gtggctctat ctcataacta atgtcacaac
     3061 cacataaatc aaattcgcgg gagaaaaagt attcccgttc ccgttcacct gtaaagggca
     3121 tgcaggtctc tcaatacgtg gtgtaacaca tatcgccttc accgaaatga tgtgaagact
     3181 tcatcatttt agcaatcaca ggttgcccta atcacttaac gctattattg ttctattgaa
     3241 gccttaacct aaacccactc acaaaacgtg gattatctgg ttagtgattc gcagcaacaa
     3301 agcgtcccgg ttcaaaacga atcgtaagac gtagtttccc ttgatgtagg gcttgccgat
     3361 ggaacaggcg gcttttttat tctccggcgt ggggggggat gctgttgaca gaaatggaaa
     3421 taagtaacat ccggaatatt acgcttattg cccggcgtct tgcctggatt aatgccaaaa
     3481 gaactcatat cgatatacca tcgtttgcag aactgctttt ctccggccaa aaagcgctgt
     3541 tcttcgaaca atataattat
//
