LOCUS       NZ_CP147134             6657 bp    DNA     circular CON 22-APR-2024
DEFINITION  Escherichia coli strain KFu015 plasmid pKFu015_4, complete sequence.
ACCESSION   NZ_CP147134
VERSION     NZ_CP147134.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39992786
            Assembly: GCF_038396395.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Gomi,R.
  TITLE     Genomic analysis of environmental Escherichia coli carrying
            transferable quinolone resistance determinants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6657)
  AUTHORS   Gomi,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2024) Department of environmental engineering,
            Kyoto University, KyotoDaigakuKatsura, Nishikyo-ku, Kyoto-city
            615-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038396395.1-RS_2024_04_20
            Annotation Date                   :: 04/20/2024 18:35:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,106
            CDSs (total)                      :: 4,987
            Genes (coding)                    :: 4,790
            CDSs (with protein)               :: 4,790
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 197
            CDSs (without protein)            :: 197
            Pseudo Genes (ambiguous residues) :: 0 of 197
            Pseudo Genes (frameshifted)       :: 59 of 197
            Pseudo Genes (incomplete)         :: 133 of 197
            Pseudo Genes (internal stop)      :: 38 of 197
            Pseudo Genes (multiple problems)  :: 35 of 197
            Pseudo Genes (short protein)      :: 5 of 197
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147134.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="KFu015"
                     /isolation_source="river water"
                     /db_xref="taxon:562"
                     /plasmid="pKFu015_4"
                     /geo_loc_name="Japan"
                     /collection_date="2011"
                     /collected_by="Ryota Gomi"
     gene            122..889
                     /locus_tag="WAZ88_RS25415"
                     /old_locus_tag="WAZ88_25415"
     CDS             122..889
                     /locus_tag="WAZ88_RS25415"
                     /old_locus_tag="WAZ88_25415"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]; GO:0043565 - sequence-specific DNA
                     binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_078206224.1"
                     /translation="MTQVCSIAFVKQSFSAEINNKIVCIRQNSLILFDQKVKSEISKNR
                     ERIIIIDIDLNTCMSFFTNTYNNAPLKCVTDECGLLIKENILPSLTATLFEFIEKTDHY
                     AASSRVWLCMSLLSLFNEHNDVNSFIFTHMKSISYKVICIINAIPEKQWQLKDVSERIY
                     MSESLIKKKLKNEGTSFTLLLIDIRMRLAKKLLSSGLYSISETAIKCGYKNTSYFISSF
                     KKYYGVTPSHHIKNTINNVNPTKVQYSNLGELY"
                     /name="helix-turn-helix domain-containing protein"
     gene            944..1087
                     /locus_tag="WAZ88_RS25420"
                     /old_locus_tag="WAZ88_25420"
     CDS             944..1087
                     /locus_tag="WAZ88_RS25420"
                     /old_locus_tag="WAZ88_25420"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_170872253.1"
                     /translation="MIKVLCDADKNIDKRLLSHLSPLGWERISLTDDYIWHQNKQVEQC
                     KF"
                     /name="hypothetical protein"
     gene            complement(1454..1612)
                     /locus_tag="WAZ88_RS25425"
                     /old_locus_tag="WAZ88_25425"
     CDS             complement(1454..1612)
                     /locus_tag="WAZ88_RS25425"
                     /old_locus_tag="WAZ88_25425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_277107729.1"
                     /translation="MHEPPVQPDRCAFSGNYRLESNPVRHDLTPLAAAIGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            complement(2263..2454)
                     /locus_tag="WAZ88_RS25430"
                     /old_locus_tag="WAZ88_25430"
     CDS             complement(2263..2454)
                     /locus_tag="WAZ88_RS25430"
                     /old_locus_tag="WAZ88_25430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_078206223.1"
                     /translation="MTKQEKTALNMARFIKSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2520..2843
                     /locus_tag="WAZ88_RS25435"
                     /old_locus_tag="WAZ88_25435"
     CDS             2520..2843
                     /locus_tag="WAZ88_RS25435"
                     /old_locus_tag="WAZ88_25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_078206222.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMTIGDELRRLRLAVREQGGR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3052..>3483
                     /locus_tag="WAZ88_RS25440"
                     /old_locus_tag="WAZ88_25440"
                     /pseudo=""
     CDS             3052..>3483
                     /locus_tag="WAZ88_RS25440"
                     /old_locus_tag="WAZ88_25440"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3521..4039
                     /locus_tag="WAZ88_RS25445"
                     /old_locus_tag="WAZ88_25445"
     CDS             3521..4039
                     /locus_tag="WAZ88_RS25445"
                     /old_locus_tag="WAZ88_25445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_078206227.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMRQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVISFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            4046..4279
                     /locus_tag="WAZ88_RS25450"
                     /old_locus_tag="WAZ88_25450"
     CDS             4046..4279
                     /locus_tag="WAZ88_RS25450"
                     /old_locus_tag="WAZ88_25450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            5201..5845
                     /locus_tag="WAZ88_RS25455"
                     /old_locus_tag="WAZ88_25455"
     CDS             5201..5845
                     /locus_tag="WAZ88_RS25455"
                     /old_locus_tag="WAZ88_25455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrD1"
                     /protein_id="WP_012634451.1"
                     /translation="MEKHFINEKFSRDQFTGNRVKNIAFSNCDFSGVDLTDTEFVDCSF
                     YDRNSLEGCDFNRAKLKNASFKSCDLSMSNFKNISALGLEISECLAQGADFRGANFMNM
                     ITTRSWFCSAYITKTNLSYANFSRVILEKCELWENRWNGTVITGAVFRGSDLSCGEFSS
                     FDWSLADFTGCDLTGGALGELDARRINLDGVKLDGEQALQLVESLGVIVHR"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrD1"
     ARG             5202..5842
                     /name="qnrD1"
                     /gene="qnrD1"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrD1"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2092..2177
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.727"
                     /coverage="100.0"
                     /evalue="1.8899999999999998e-34"
     Typing          complement(1541..2068)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.322"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          4952..5107
                     /name="Col3M"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.089"
                     /coverage="100.0"
                     /evalue="2.29e-71"
     Typing          2834..4383
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.229"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gctatatgta tagtattaaa tatgctaatt taattagttg atgtataaat agctatttat
       61 ataaggatta aaaatccttg ttgcttataa ttatcacgaa aataatcaag gaatagatat
      121 tatgacacaa gtttgctcta tagcctttgt taaacaatca ttttccgctg aaataaacaa
      181 taagatagtc tgtataagac aaaattcact tattcttttt gatcaaaaag taaaaagcga
      241 aatttcgaag aatagagaaa ggataataat tatcgacatc gatcttaata cgtgtatgtc
      301 attttttact aacacgtaca ataacgcacc gttaaagtgc gttacagatg agtgtggttt
      361 gcttatcaaa gagaatattt tgccatcatt aacggcaacc ctctttgaat ttatagaaaa
      421 aacagatcat tacgctgcga gttcaagggt ctggttatgt atgtctctgt tatcactatt
      481 taatgaacat aatgacgtta atagctttat ctttacgcat atgaaatcca tctcatataa
      541 agttatttgt atcattaacg caattccaga aaaacaatgg cagttaaaag atgtttctga
      601 gcgaatatat atgagtgaaa gtttgataaa gaaaaagtta aaaaatgaag gtacttcgtt
      661 cacattgcta ttaattgaca ttcgaatgcg tctcgccaaa aaattgcttt catcaggatt
      721 gtattctatt agcgagacag caattaagtg tgggtataaa aatacgtcat attttatatc
      781 ttcatttaaa aaatattatg gagtaacacc atcacaccat ataaaaaaca ctattaataa
      841 tgttaatcca acaaaggttc aatattctaa cctaggcgag ctttattaaa taaatcagat
      901 tttcgggtgg ttctctctgt atccggttgg atttcaggca cgggtgatca aggtgctgtg
      961 cgacgcggac aagaacatcg acaagcgact attatcgcat ctttcgccgc tgggctggga
     1021 gcgcatcagt ctgactgatg actacatctg gcaccagaac aagcaggtcg agcaatgcaa
     1081 gttttgaccg ttacgaatgc ctggcgaggc ctagcgtacg attttttccg tttcgtgagc
     1141 tgagccttat ttgacgataa atcagagtca ttactaggta gtttatcttg gaagtttgcc
     1201 tgaatgagtt cagtgatttt ataggcgaga tgccgacatt attgttgtcc atgaagtcgg
     1261 agtgatgaaa agatcaaagg atcttcttga gatccttttt tcctgcgcgt aatctgctgc
     1321 ctgtaaacaa aaaaaccacc gctaccaacg gtggtttgtt tgccggaatt gagagctacc
     1381 aactctttct tccgaaggta actggcttag gtggagcgca gataccaaaa cagtccttct
     1441 agtgtagcct cagttaggcc accacttcaa gaactcttaa tatctcaatc caccttgtcc
     1501 agttaccaat ggctgctgcc agtggcgtta agtcgtgtct taccgggttg gactcaagac
     1561 gatagttacc ggaaaaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1621 gcttggagcg aacgacctac accgagccga gataccaaca gcgtgagcta tgagaaagcg
     1681 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aaacggcagg gtcggaacag
     1741 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttaagtc ctgtcgggtt
     1801 tcgccacctc tgacttgagc gtcgtttttt gtgatgctcg tcaggggggc ggagcctatg
     1861 gaaaaacgcc agtggcgcgg cctttttgct cacatgttct ttccggcctt atcccctgat
     1921 tctgtggata accgtattac cgtctctgag tgagtcagta ccgctcgccg cagccgaacg
     1981 accgagcgta gcgagtcagt gagcgaggaa gcgggaaagt gcccggatgt gcattttctc
     2041 cttacgcatc tgtgcggcgt ttcacaccgg ccatgatgcg cttttcatac aatcagcacg
     2101 atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     2161 gccccgacac ccgctaaaac ccgctgacgc gccctggcgg gcttatctgc tcccggcatc
     2221 tgctcacaga caagctgtga ccgtctccgg gagctgcctg tgtcagaggt tttcaccgtc
     2281 atccccgaag cgtgctaggc agctgcggta aagctcatcg gcgtggtcgt gaagcgattc
     2341 acagatgtct gcctgttcat ctgcgtccag ttcgttgagc ttctccagca gcgttaatgt
     2401 ctggcttttg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc
     2461 ctccgtgtaa ggggggattt ctgttcatgg gggtaatgat accgatgaaa cgcgagagga
     2521 tgctcacgat acgggttact gatgatgaac atgcccggtt actggagcgc tgtgagggta
     2581 aacaactggc ggtatggatg cggcgggtct gcctgggtga gcctgtcgcc cgttccggga
     2641 agctgccgac actggcaccg ccgttactgc gtcagctggc cgccatcgga aataacctga
     2701 atcagacagc gaggaaggtg aacagcgggc agtggtcttc cggggaccgg gttcaggtgg
     2761 tggccgcact gatgaccatc ggggatgagc tgcgccggtt gcgtcttgcc gtcagggaac
     2821 agggagggcg ggatgatagt taagtttcat gccaggggga aaggtggtgg cagtggtccg
     2881 gttgattacc tgctgggaag ggagcgtaac cgggaagggg caacggtgct tcagggtaat
     2941 ccggaagaag tccgggaact catcgatgcc acgccatttg cgaagaaata tacgtcaggt
     3001 gttctgtcgt tcgcggagaa ggagctgccg cctggagggc gtgaaaaagt gatggcgagc
     3061 tttgagcgtg tactgatgcc cggtctcgaa aagaatcagt acagcatcct gtgggtggag
     3121 caccaggaca agggacggct tgagctgaat tttgtcattc cgaacatgga gttacagagc
     3181 ggaaaacgcc tccagccgta ctacgaccgg gcagacaggc cgagaattga tgcctggcag
     3241 acgctggtga atcaccatta cgggctgcat gaccccaacg ccccggagaa ccgccggata
     3301 ctgactctcc ctgataacct gccggaaacg aaacaggcgc ttgctgagag cgtcacacgc
     3361 gggatagacg ctctttatca tgtcggggag ataaaaggcc gtcaggatgt gattcaggcg
     3421 ctcacggaag cggggcttga agtggtcagg gtgacgcgaa gcagtatcag cattgcagac
     3481 ccgaacggcg ggaggaatat cagactgaaa ggagcgtttt atgagcaatc ttttacagac
     3541 gggcgcggag ttcgagaaaa agctgaaaga gagagccgaa tctacagaga aaatgctgaa
     3601 caacgagttc aggaggctcg gcgaatctgt aagcgaggct gtgacatcaa acgagacgaa
     3661 aatcagagac gctatagccc tgttcacagc ctcgacagag gaatcgctgg agaaacaccg
     3721 ggaaggggtg aaagaggcga tgaggcagca caggaaggac gtgttaaagc tggcagggaa
     3781 tacgggcatg atgttactgg ggatagtctt tctcctgttt accgcgagtg gcgggacgct
     3841 ctggtatctt ggcgggagga tacaggcgaa cctggaagat atcaggaagc aggaagagac
     3901 attgcagaaa ctgaacgcga agacatgggg cgtggagttt gtgcaggacg ggaacaggaa
     3961 atttctggtc cttccttacg ggaagtcagc agaggtgatt tcctttcagg ggaaggaatg
     4021 ggtacatctg aaggagtgac acagagtgac agagctggaa acacatttgc tgaacgcctt
     4081 agagcagctg caacaggact atatgcagcg gctgagcgaa tgggagagcg ccttcgtgga
     4141 attgcagaag atgttttcgc ttacgcaacg ggacaacgcg atgctgaacg agcgggtcat
     4201 gcagttgagt cagcaggtgc agcacttgag cgagcagaca gaacgcttga gtcagttata
     4261 cagcgagaac tggagataag agaggaacgg ctgatacagg agcgcgaaca tgcgttatcc
     4321 ctggaacggg agcgtcagcc ggaaatacag gaacgcacgc tggatggccc ttcgctggga
     4381 tggtgaaacc atgaaaaatg gcagcttcag tggattaaga gggggtaatg tggcctgtac
     4441 catctggttg cataggtatt catacggtta aaatttatca ggcgcgatcg cggcagtttt
     4501 tcgggtggtt tgttgccatt tttacctgtc tgctgccgtg atcgcgctga acgcgtttta
     4561 gcggtgcgcg taatgcgacg ttatggtaaa tacgccgctt agctctattt ttgggtttgt
     4621 tctggactgt tttttttgta ctttttgtat attgataaat attttactaa caacatcgac
     4681 aaaatcatta tatcgagcta ggggtctgaa ggccaataga acgaaaacgt acgttagtga
     4741 agtaactgtc tgatatatcg aaacataatg tacattggaa aacgccatca aaacggtgtc
     4801 tttttaatcg aaaattggca cttaacggac tttcttgtct actaatcgat ttttcagacg
     4861 ataattttcc tcttaccgta cgttttcgta cggttggacc tcaaacccca ctttcagcga
     4921 tttcgcatcg ttttacttgt cagtttgcac gatgttaaaa acgcactcag aagcccgcaa
     4981 aaccgttcta aactttcagc gtagatttgg gtgttttata gggtgatcgt gtcttaaaat
     5041 cgtttcgagg ggctttaatg gggatgaacg ggaggttttt gctaactaac tcgcccgttt
     5101 aacataaaag ggattgtatg aatcaatttt agctagagtt taaggttgtt caaattaatg
     5161 tacaatgatt gcactgtata aataaccagg tgtagcatgt atggaaaagc actttatcaa
     5221 tgaaaagttt tcacgagatc aatttacggg gaatagagtt aaaaatattg ccttttcaaa
     5281 ttgtgatttt tcaggggttg atttaactga tactgaattt gttgattgta gtttttacga
     5341 caggaatagc ttggaagggt gtgattttaa tagagccaaa ctaaaaaacg ctagctttaa
     5401 aagctgcgat ttatcaatga gtaattttaa aaacattagc gccttaggtc ttgaaattag
     5461 tgagtgttta gctcaaggag ctgattttcg aggggctaat tttatgaata tgataactac
     5521 aaggtcatgg ttttgtagtg cttatataac caagacaaat cttagttacg ctaatttttc
     5581 tagagtcata ttagaaaagt gcgaactgtg ggaaaatcgc tggaatggca ctgtgataac
     5641 tggcgccgtg tttcgtggct ccgatctttc ttgtggggag ttttcatcgt ttgattggtc
     5701 tttggctgat tttactggtt gtgatttaac gggtggggcg cttggcgagc ttgatgcaag
     5761 gcgaattaat ttagatggag tgaagttgga tggagagcag gcgcttcagc ttgttgagag
     5821 tttaggtgtt attgttcacc gataaaatct aggtaaaaaa cgcctaatgc cccaatgtgg
     5881 tactaatcaa aacggagcga agcgacatct tcatcttgtc tttgcttttc tccatgatca
     5941 gttattgggt catggtctct tagctcgtta atctggctaa gtttggttag gttgggcttc
     6001 tttcgacact tcgcaatcca agaaatccct agtccgttga ttggtgctct tatcaggtac
     6061 gcactgtaat ctttcgatac cgcagcgtga ttcggaggta gagccggaat ttcgttttcc
     6121 ggtacgtttt actaccacaa tttgcctgtt tatcctttcg gagaccgggc taagtccgag
     6181 tcaatcaagt ggtgcatttg ggttaatgtc gttggctggt atttaatgag aacgtgacaa
     6241 gcgcctctca cgttgctaag attggattgc gacgtctaat ctgagcaacg aatgcacaag
     6301 atttagagta ccagagccct gtgatgctgt cacggggctt tgttacatct ggagcttggt
     6361 aaacctcata aattcaattc gcaacgctcg ccgcaaggcg tgaacttgtt tcacgtcgag
     6421 gaagcggaat ttctcgaaag ccttataggg gtctgaaggc caatagaacg aaaacgtacg
     6481 ttagtgaagt aactgtctga tatatcgaaa cataatgtac attggaaaac gccatcaaaa
     6541 cggtgtcttt ttaatcgaaa attggcactt aacggacttt cttgtctact aatcgatttt
     6601 tcagacgata attttcctct taccgtacgt tttcgtacgg ttggacctca aacccca
//
