LOCUS       NZ_CP147094             2175 bp    DNA     circular CON 22-APR-2024
DEFINITION  Escherichia coli strain KTa005 plasmid pKTa005_10, complete
            sequence.
ACCESSION   NZ_CP147094
VERSION     NZ_CP147094.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39992792
            Assembly: GCF_038396305.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Gomi,R.
  TITLE     Genomic analysis of environmental Escherichia coli carrying
            transferable quinolone resistance determinants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Gomi,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2024) Department of environmental engineering,
            Kyoto University, KyotoDaigakuKatsura, Nishikyo-ku, Kyoto-city
            615-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038396305.1-RS_2024_04_20
            Annotation Date                   :: 04/20/2024 18:14:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,001
            CDSs (total)                      :: 4,881
            Genes (coding)                    :: 4,637
            CDSs (with protein)               :: 4,637
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 59 of 244
            Pseudo Genes (incomplete)         :: 183 of 244
            Pseudo Genes (internal stop)      :: 41 of 244
            Pseudo Genes (multiple problems)  :: 40 of 244
            Pseudo Genes (short protein)      :: 4 of 244
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147094.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="KTa005"
                     /isolation_source="river water"
                     /db_xref="taxon:562"
                     /plasmid="pKTa005_10"
                     /geo_loc_name="Japan"
                     /collection_date="2013"
                     /collected_by="Ryota Gomi"
     gene            complement(join(2023..2175,1..273))
                     /locus_tag="WAZ86_RS24995"
                     /old_locus_tag="WAZ86_24990"
     CDS             complement(join(2023..2175,1..273))
                     /locus_tag="WAZ86_RS24995"
                     /old_locus_tag="WAZ86_24990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676030.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="WP_015328245.1"
                     /translation="MGSYVDQSLTRNESVISRAQTSWIPTIIPVIIGILLLPFYGLGLL
                     IIIPVLLRVWSTELALTNQRVIAKVGLIRRNTVELRIDKVESLGIHQGILGRIFNYGSI
                     VIKGTGGTNTPIPNIKAPMQFRSIVNNHIEEMNSKQQ"
                     /name="PH domain-containing protein"
     gene            complement(673..834)
                     /locus_tag="WAZ86_RS25000"
                     /old_locus_tag="WAZ86_24995"
     CDS             complement(673..834)
                     /locus_tag="WAZ86_RS25000"
                     /old_locus_tag="WAZ86_24995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045343414.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSLE
                     VMRLLRLN"
                     /name="hypothetical protein"
     gene            1521..1757
                     /locus_tag="WAZ86_RS25005"
                     /old_locus_tag="WAZ86_25000"
     CDS             1521..1757
                     /locus_tag="WAZ86_RS25005"
                     /old_locus_tag="WAZ86_25000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016230891.1"
                     /translation="MTRLTNLTPAEKKFIDDAIAAAERAAGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     Typing          1185..1357
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.659"
                     /coverage="100.0"
                     /evalue="9.74e-41"
     Typing          complement(722..1238)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.692"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atgtatgcca aggctttcaa ctttatcaat acggagttcc accgtattgc gtcggatcaa
       61 acccactttt gcgatgactc tttggttagt taatgcaagc tccgttgacc aaactctaag
      121 gagcacaggg attattataa gtaaccctaa gccgtaaaaa ggcagcaaca agattccgat
      181 aattactggg atgattgttg gaatccagga ggtttgagcg cgagatatta cggattcatt
      241 tcttgtcaat gattggtcta cgtagcttcc cataaggtta tatccattta gaaaaaatag
      301 gatttttccc tttttaaggc gaatcagcaa tagaaacttt atgtctccgt ggtaggtata
      361 caaaatttac ggagagcgta gcgaccgtcg cacagttcgg ttctttcctt tcgtgccgca
      421 actggcttga ttctgtgatg gtggtggtgg ctgctgctat gtctcatata ctcttggcgt
      481 tgcaatcctt tatcccatat ctgtcgaata tcacagatga tcttcttgag atcttgtccg
      541 ttgcgtcgta atctcttgct ctggaaacga aaaaaccgcc ctgaaaggcg gtttttcgaa
      601 ggttctcaga gctaccaact ctttgaaccg cggtaactgg cttggaggag cgcagtaacc
      661 aaatctgtcc tttcagttta gccttaacag gcgcataact tcaagactaa ctcctctaaa
      721 tccgttacca atggctaccg ccactggcga tttgtcgtgt ctttccgggt tggactcaag
      781 ttgatagtta ccggaacggg cgtggtagtc ggactgaacg gggggttcgt gcatacagtc
      841 cagcttggag cgaactgcct acccggaact gagtgtcagg cgtggaatga gataaacgcg
      901 gccataacag cggaatgaca ccggtacacc gaaaggcagg aacaggagag cgcacgaggg
      961 agctaccagg gggaaacgcc tgggatcttt atagtcctgt cgggtttcgc caccactgat
     1021 ttgagcgtca gattctgtga tgcttgtcag gggggcggag cctatggaaa aacggcagcg
     1081 ccgcatcctt tttctgcccc gtttgtcatg gcatatgcct ttgctcttct ccgtgtgcag
     1141 gtaacgcccg ccgcagccga acggcaggag cgtccgcgac tgagtgagcg aggaggcgtc
     1201 atatcctctg aacttccatt ttctgcctta cgcccgcagc tgcattttcc tctgtcacat
     1261 gaagcacttt cctgattacc gcactcatgc caacataata agccagtata cactccgcta
     1321 gcgctacgtg actggttcag ggctgcgccc cgaaacccgc taaatccact gacgcgcctg
     1381 cggcttgtcc aacgcctcac ggcaccggat taaaatccgc tcccgttcac cgtttctgac
     1441 tgcctttcac cgtcgcttac gcttcatcgt tcctactgtg tctggcagac tgctgggact
     1501 attctcatac ggagataaac atgacgagac ttacaaacct gacccccgct gagaaaaagt
     1561 ttatcgacga cgctatagcc gctgccgaac gtgcggccgg gaagaagctc aatcagccta
     1621 accggcacat cgttctgaac agagcgcgtg cgcagattga gtcgcagcgt tacgccgacc
     1681 gccagcgtgc attacgtgag gatgagcggc agcagtccga ttttgcctgg tcacgtccgc
     1741 gcgcgccgcg ccgctgattg cggattttgg cggtttagtg gtgctgacgc tgcgaaatca
     1801 cctgcgattc acccgcgtgc ataacgattc atgcgctcaa aaatcaccag agcgtttctg
     1861 aacgtttttc aggaggtcat acgtgttctt ttgcgtatta gggcggagaa acgccctgag
     1921 agcctctgag gcggtgcggg caacgggtgt tatggtaaat ccagctgatt ggcaggtaca
     1981 aaaaaagccc cgtagggctt tttttgtaat gctacgagga agctactgct gcttagaatt
     2041 catttcctcg atatgattgt tcacaatcga tctgaattgc attggcgcct tgatgtttgg
     2101 aattggagtg ttggttccgc cagtgccttt aatcacgata gacccatagt taaagattcg
     2161 tcccagtatc ccttg
//
