LOCUS       NZ_CP147092             3363 bp    DNA     circular CON 22-APR-2024
DEFINITION  Escherichia coli strain KTa005 plasmid pKTa005_8, complete sequence.
ACCESSION   NZ_CP147092
VERSION     NZ_CP147092.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39992792
            Assembly: GCF_038396305.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Gomi,R.
  TITLE     Genomic analysis of environmental Escherichia coli carrying
            transferable quinolone resistance determinants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Gomi,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2024) Department of environmental engineering,
            Kyoto University, KyotoDaigakuKatsura, Nishikyo-ku, Kyoto-city
            615-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038396305.1-RS_2024_04_20
            Annotation Date                   :: 04/20/2024 18:14:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,001
            CDSs (total)                      :: 4,881
            Genes (coding)                    :: 4,637
            CDSs (with protein)               :: 4,637
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 59 of 244
            Pseudo Genes (incomplete)         :: 183 of 244
            Pseudo Genes (internal stop)      :: 41 of 244
            Pseudo Genes (multiple problems)  :: 40 of 244
            Pseudo Genes (short protein)      :: 4 of 244
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147092.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="KTa005"
                     /isolation_source="river water"
                     /db_xref="taxon:562"
                     /plasmid="pKTa005_8"
                     /geo_loc_name="Japan"
                     /collection_date="2013"
                     /collected_by="Ryota Gomi"
     gene            complement(join(2845..3363,1..417))
                     /locus_tag="WAZ86_RS24955"
                     /old_locus_tag="WAZ86_24945"
     CDS             complement(join(2845..3363,1..417))
                     /locus_tag="WAZ86_RS24955"
                     /old_locus_tag="WAZ86_24945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_045626452.1"
                     /translation="MATYHLSVKFGGKGKALAHASYITREDKFSQRQDLEHTEHGNMPE
                     WARDEPAHFWQAADAFERANGSTYREIEIALPRELNEAQRLALVRDFVKQETGDKHAWT
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERSPEQYFRRYNAKFPARGGARKDSGHNL
                     TPTEQKQALKALRERWERTHNAHMKRYGFEQGFIDSRSLKTQGIDRKPEQHLGFEAAGR
                     LDDSQRQDIKDSRTFSVVDVPEQAKHIDHSDRSWMNASGIEVNRKELVRSLTHDISQQY
                     GREMAARVTKQGNVEIERVKSLSRGFSISR"
                     /name="MobA/MobL family protein"
     gene            584..970
                     /locus_tag="WAZ86_RS24960"
                     /old_locus_tag="WAZ86_24950"
     CDS             584..970
                     /locus_tag="WAZ86_RS24960"
                     /old_locus_tag="WAZ86_24950"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045282373.1"
                     /translation="MRISNVEWLKKRIAFIRKLGEKTARQRQIIDLLDDEASLSETERR
                     LLHVLATAEKNALQERDAAQKKAVQARISGQKNRRERNHRLFLAAGLMIDAGLVDTKTG
                     ELNFDHEKLLTALKRVRYDLETSA"
                     /name="hypothetical protein"
     gene            complement(2312..2842)
                     /locus_tag="WAZ86_RS24965"
                     /old_locus_tag="WAZ86_24960"
     CDS             complement(2312..2842)
                     /locus_tag="WAZ86_RS24965"
                     /old_locus_tag="WAZ86_24960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012634455.1"
                     /translation="MASTEEKLLTLLLSAEHLKEAAKEQQKAVADSLAVARETLKGYRE
                     ISDSVAQQVRDEVRQEVQKMDVAGLIGERISADFQRLEKSVGNMKINADALDKEISIIR
                     SNIIAEYNTLRGYSWKWFAGVAVAFVVLIFATSWFVKTELQDNYNASAAVYQKVADLEK
                     LLTTEQKPEKKKN"
                     /name="hypothetical protein"
     Typing          complement(2..417)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.928"
                     /coverage="100.0"
                     /evalue="4.58e-81"
ORIGIN
        1 ttcgggactg cgctcaatac cgtcattgac gcgctggctc atcatgatat gggcgtgcgg
       61 ctgttcgccg ccgtcaatgc tggctttcgg attgtggatc gcaaacgtcc atgcatgttt
      121 gtcgcccgtt tcctgcttca caaaatcccg gacaagcgcc agccgctgcg cttcattcag
      181 ctcgcggggt agagcgatct caatttccct gtacgttgaa ccgttggcac gttcgaacgc
      241 atccgcagcc tgccagaaat gggcgggttc atctctcgcc cattccggca tattgccgtg
      301 ttcggtgtgc tcgagatcct ggcgctggct gaacttatct tcccgcgtaa tgtaactggc
      361 atgagcaagg gctttaccct tgccgccaaa tttcacgctg agatgatacg ttgccatgtt
      421 gtgtcagccc ccggcagggc gcactgtttc tgcccgcagg gagaaacata taagtgcgct
      481 ttactctaat ttaaagaatt atgagtaaag ataagtcatg cgacttctga ccgcaagtta
      541 tatactttcc ccgtcggaaa tatcgggatg gggaagttaa acgatgcgga tcagtaacgt
      601 cgaatggctt aaaaagcgca tagccttcat cagaaagctg ggagaaaaaa cggcacggca
      661 acggcagatt atcgatctgc tggatgatga agcttcgctc agtgagactg aaagacgact
      721 gctccacgtt ctggcaaccg ctgaaaaaaa cgcgttgcag gaacgggatg ccgcacagaa
      781 aaaggcggtt caggcgcgga tttcagggca gaaaaaccgc agggaacgca atcacagact
      841 gtttctggcg gctggtctga tgattgacgc gggactggtt gatacaaaaa cgggtgagtt
      901 gaattttgac cacgaaaaat tactgactgc gctgaaacgg gtacgctacg atctggaaac
      961 atcggcgtga acgtgcattc tgcaaggccg gagaaatgcc gcttcctcgc tcactgactc
     1021 gcgtcgctcg gtcgttcggc tgcggcgagc ggtagggatt ttcggatagg agcatgaatt
     1081 ttcttgctcc agatgtacaa aagccccgtg acagaatcac ggggcttgag tactctaatt
     1141 cttgcagata cctgccagga tcagacgtca caatcaaatc ctagcagggc tggctaggaa
     1201 tgtcactacc agcttaatac cattcaacga cattaaaccc atatctgcac cttgactctc
     1261 aggtctacgc cccggactcc ggcaggataa acaggcaagc ggtgggaacc tgaccagctc
     1321 gggaaagaag ctggctctac ccggtctcac gttaaccaat aacgtacctg ataaagtgag
     1381 agtgaaggca gcggttgcgg tgaagcgtga ttgccaaggt ctggcgcaac acccacaaaa
     1441 tcgaagcagc atacgaacgg cctgatgctg gtcgcttcgc tccccgtttt ctggtgtacc
     1501 caccacgggg gcattaggct ttctttgccc taatatgggt ttgcgactct caaatccggg
     1561 taatacgtgg tgtaacacat atcgccttca ccgaaatgat gtgaagactt catcatttta
     1621 gcaatcacag gttgccctaa tcacttaacg ctattattgt tctattgaag ccttaaccta
     1681 aacccactca caaaacgtgg attatctggt tagtgattcg cagcaacaaa gcgtcccggt
     1741 tcaaaacgaa tcgtaagacg tagtttccct tgatgtaggg cttgccgatg gaacaggcgg
     1801 cttttttatt ctccggcgtg gggggggatg ctgttgacag aaatggaaat aagtaacatc
     1861 cggaatatta cgcttattgc ccggcgtctt gcctggatta atgccaaaag aactcatatc
     1921 gatataccat cgtttgcaga actgcttttc tccggccaaa aagcgctgtt cttcgaacaa
     1981 tataattatt ttcactcacg acggtacaca acggcaggca cgtcaaagcc accggattta
     2041 ccataacatc tcttgcccgc accgctgaaa cgcgctcagg gcggttttac gtcctgagcc
     2101 ataaaaaccc ccggcttctc accgaaaacc gctcagaatc gcataccgtg attttcagcc
     2161 ccatgcataa ccatgcacct gactgactgg cgagaacttc cggcctgaat tcaggccgga
     2221 agtctcaaaa gacgccccaa actcggctga aaacggtgaa cgggtgcgca atttattgtg
     2281 cgaacgtgag gcgttggaca agcgcagcgc gtcagttctt tttcttttcc ggtttctgtt
     2341 ccgttgtcag cagcttttca aggtctgcca ctttctgata gacggcagca gacgcgttgt
     2401 aattgtcctg tagttccgtt ttcacaaacc agcttgtggc aaatatcagt acgacaaagg
     2461 caaccgcaac cccagcaaac cacttccagc tgtaaccccg cagcgtgttg tactccgcga
     2521 tgatgttact gcggataatg gaaatttcct tgtccagcgc gtccgcattg attttcatgt
     2581 tcccgacgga tttttccaga cgctgaaaat cagcgctgat acgctcgcca atcagccccg
     2641 caacatccat tttctgcact tcctgccgga cttcatccct tacctgctgt gccacactgt
     2701 ccgatatttc ccggtagcct ttcagggttt cccttgccac cgccagcgaa tccgccacgg
     2761 ctttctgctg ctcttttgcg gcctctttca gatgctccgc cgacagcagg agtgtgagaa
     2821 gtttttcttc ggtgctcgcc atagttacct gcttatggaa aatccgcgtg acagggattt
     2881 cacgcgctct atttcaacat ttccctgttt tgtgacccgt gcagccatct cccgcccgta
     2941 ctgctgcgag atatcatgcg tcagagatct gaccagttcc ttccggttta cctcgatacc
     3001 gctggcattc atccagctac ggtcgctgtg gtcgatatgt ttcgcctgtt caggcacatc
     3061 cacgactgaa aacgtccggc tgtctttaat atcctgcctc tggctgtcgt ccagtcgtcc
     3121 ggctgcttca aaaccgagat gctgttccgg tttccggtcg ataccctgtg ttttcagtga
     3181 ccggctatca ataaaaccct gctcaaagcc atatcgtttc atatgcgcgt tgtgcgtacg
     3241 ctcccagcgc tcccgcaggg ctttcagtgc ctgtttttgt tctgtagggg ttaaattatg
     3301 ccctgagtcc ttgcgcgcgc cgccacgcgc cggaaatttg gcgttatagc gcctgaaata
     3361 ctg
//
