LOCUS       NZ_CP147075             8162 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Escherichia coli strain JSWP021 plasmid pJSWP021_4, complete
            sequence.
ACCESSION   NZ_CP147075
VERSION     NZ_CP147075.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39992796
            Assembly: GCF_039645365.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8162)
  AUTHORS   Gomi,R.
  TITLE     Genomic analysis of environmental Escherichia coli carrying
            transferable quinolone resistance determinants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8162)
  AUTHORS   Gomi,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2024) Department of environmental engineering,
            Kyoto University, KyotoDaigakuKatsura, Nishikyo-ku, Kyoto-city
            615-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_039645365.1-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 00:04:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,858
            CDSs (total)                      :: 4,735
            Genes (coding)                    :: 4,599
            CDSs (with protein)               :: 4,599
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 56 of 136
            Pseudo Genes (incomplete)         :: 80 of 136
            Pseudo Genes (internal stop)      :: 22 of 136
            Pseudo Genes (multiple problems)  :: 19 of 136
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147075.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="JSWP021"
                     /isolation_source="municipal wastewater"
                     /db_xref="taxon:562"
                     /plasmid="pJSWP021_4"
                     /geo_loc_name="Japan"
                     /collection_date="2015"
                     /collected_by="Ryota Gomi"
     gene            complement(join(6370..8162,1..367))
                     /gene="mobQ"
                     /locus_tag="WAZ80_RS22545"
                     /old_locus_tag="WAZ80_22560"
     CDS             complement(join(6370..8162,1..367))
                     /gene="mobQ"
                     /locus_tag="WAZ80_RS22545"
                     /old_locus_tag="WAZ80_22560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_023908709.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDRERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            546..800
                     /gene="mobC"
                     /locus_tag="WAZ80_RS22550"
                     /old_locus_tag="WAZ80_22565"
     CDS             546..800
                     /gene="mobC"
                     /locus_tag="WAZ80_RS22550"
                     /old_locus_tag="WAZ80_22565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            1333..2034
                     /locus_tag="WAZ80_RS22555"
                     /old_locus_tag="WAZ80_22570"
     CDS             1333..2034
                     /locus_tag="WAZ80_RS22555"
                     /old_locus_tag="WAZ80_22570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_023908708.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     ITLDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(2289..4448)
                     /gene="mobQ"
                     /locus_tag="WAZ80_RS22560"
                     /old_locus_tag="WAZ80_22575"
     CDS             complement(2289..4448)
                     /gene="mobQ"
                     /locus_tag="WAZ80_RS22560"
                     /old_locus_tag="WAZ80_22575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_023908709.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDRERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            4627..4881
                     /gene="mobC"
                     /locus_tag="WAZ80_RS22565"
                     /old_locus_tag="WAZ80_22580"
     CDS             4627..4881
                     /gene="mobC"
                     /locus_tag="WAZ80_RS22565"
                     /old_locus_tag="WAZ80_22580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            5414..6115
                     /locus_tag="WAZ80_RS22570"
                     /old_locus_tag="WAZ80_22585"
     CDS             5414..6115
                     /locus_tag="WAZ80_RS22570"
                     /old_locus_tag="WAZ80_22585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_023908708.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     ITLDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(10..368)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          369..545
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          complement(4091..4449)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4450..4626
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          1334..2034
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.858"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5415..6115
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.858"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2293..4535)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.733"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6374..8160)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.832"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccacatcagc aatcatgccc tgactgacaa agttttctct gacaaaggcg agaacggttt
       61 cccgtgcggc atcctgcggt agttcgcggg ggatggcgag ttcaatttcc cgcgcaagtt
      121 gtgagttctt ccggcgttcg gccttctcaa cggcattcca gagttcggca cggttcgtac
      181 accaggcagg ggctgtcgcg ggagtcagaa tcacagcttc agccacaccg cttttgcggg
      241 tgtagtcgtg agtcagtccg gtacgctcat caagcatcag tgaaccggaa cgataggcag
      301 cagaagcaac ggcgctgtag ccgctgtttc tggagatgat tttcatgctg agatgataaa
      361 tcgccatttc tgccgtaatg cctttttgtg gttttctttc agccccgcag ggcgcacaca
      421 atcgaaacgg agtggagatt gtataagtgc gcacttcgtg attttccgtg cattatgcca
      481 gctcagtttt taaactacca gtgacgcgct aaaatgcgat ttttcatcgc gcacggagtt
      541 acatcatgag cagaacactc gaacagaaga ttgctgatgc tgaggccaga ttacagcgcc
      601 tgaaggcgaa gagcaggagt ctggacacag cgcagaaggt cattgtgggt gcagcattgc
      661 tggcaaaggt cagaaagccg gaggaggtgc agttgcgtgc ctggttactc cagttcctga
      721 aggcagaggt gacacgacag gctgatgtga cgcgcatact gccgctgatt aacgagctgg
      781 aagcgctgcc aggacagtga ttgtatcgtc cgctcgccgc agtcccagcg accgagcgtt
      841 agcgagcgag tgggcgagga agcggaagtg aatccgtcgc ctgattgagc acatacgcac
      901 ggtttttcgg gaggtgatat gcgctgaacc cgcatgaata ctggatttcc acagggttgg
      961 agggggtgta ttcggaactt gcaattctgc ttttttgcaa cgcattgttt ttattggttt
     1021 tttgttttca gcactccaag ttatccacag gctcgtcgtt aagtttttag atcttgtttt
     1081 taagatcttg tttttaatac ttgttttagc gccaaaattc cttatagaaa acaaaatgtt
     1141 acaacgctat aggtaatatg atttaaggta aaggtaatat gatttaaggt gcttgggtaa
     1201 tatgatttaa ggtaaaggca atatgattta aggtaatatt cacagaaaaa gggatattaa
     1261 aataaaaagg cattgccttt cgttgagggt atgtttatct tttattcatt accttttggg
     1321 gagggatgct ctatgtcgga tctaattgcc tataaatcca atgcgctggt tgaagccagt
     1381 tataaattga ccctacagga acagcgattt ttgttacttt gcatcggtcg attgaagtct
     1441 ggggctgatg cagaatctcc aaaattgcaa aagacaatga ccattacagc tgcggaatat
     1501 ttcgattcat tccctgacat ggggcggaaa aatgccgagg ttcagttaca ggaagcaatt
     1561 gatcgtttgt gggacaggtc aatcattctc aaggatgacg agaaacgtga ggagttccgc
     1621 tggatccagt atcgggcaca gtacgctaaa ggcgaggcca gagcacaaat aacattttct
     1681 gacgcagtaa tgccttattt gacacaacta caagggcaat ttactagagt tgtaatcaag
     1741 aatatatcta gattatcaag aagttacagt ataaggatat acgaactcct ccagcagttc
     1801 cgcagtactg gcgaacgtat tatcactctc gatgattttc gctcaatgct tgatatagag
     1861 cataaatatc aaacatataa atcacttaac cagcaattat taagaccctg cattgacgaa
     1921 ctgaacaaaa aaagcgacct ggctgtaacg gttgagacga tcaaaaaggg gcgcacggtt
     1981 gtagccctgc attttcgctt caaggaagac aaacaaatca agatgacgat ctaaaatcga
     2041 ttttaaggca cctaaaacgc tccctgccgc gttttttttg tgttagtagt gcgaaggtac
     2101 tattgaagtg aaaaattgcg tcaaaagcgc tctaatgcag cgaaaaaaga acccccattt
     2161 ttgggggcgg aaaaacaaaa ctatgcttgc attatgtgtg tgcttcttgc attcatccct
     2221 gtcgatgaac atcgaagaac atcgacgaac ataacacgat gttaaacaaa acaaaatatt
     2281 gttcacattc actgtgacca accgcgatca tgtctcctct cggtggcgtg tgttcgtgca
     2341 tcttcctcgc gccgccattc gtcccaggta cgattaaaac ccagcgagcg gaacagatga
     2401 tccggctgtt tttctggtgt gttgacggca gcaaaaagcg gtgcctcacg ggttttcaga
     2461 tgaaactcca taagttccgg cgcaggtgat atgtggctgt ctgcatcgtg atttacggca
     2521 acaccatacc ctgcataccg taacccctta tcggtcagtg cactgatgac cagctggctg
     2581 ttatcggctg agggaacggc aaacgcggga tacagcgtac cattcagccg gacaacaccg
     2641 cccagagagt catccacgcc catgaccagc aaaagctgaa gctggcgggt ttcccggtcg
     2701 acagaactca ttaccggcat cagcgtcggt agcagacgtc cctgcgtaaa atccaccttc
     2761 cttgctgcca gtgatggcat ggctggctgg cggtcttctc caagacagaa agactccggc
     2821 catgtcttcg gctggcggag attgcgggta tattgcggcc atgtctggca aacctgcgtc
     2881 agaagttgct caaaggtcgc agaggaataa acacgttgca gtggtggatg ttgaccgatt
     2941 tcgcttcggt cacagatata accactcctg tcacgcatta aatcaacacg ctcaaagatt
     3001 ctggtgcggt atttctccag ctcgttcccc cagagcgtgt attctccgtt ccggttgtga
     3061 agcagaaggt aacaggtcat ttccccgtta ccaggcatgg gggcctcgcc ataccccgta
     3121 acgactgcgc tcagtacccc tgaatccata acggcattgt catttttcct gagctgttcc
     3181 gctcgtcgct ccgcgcgact acgttctcta tcctcctgct ccttccggcg caattcctct
     3241 tcacgtttaa gcaactggac tctgagagct gcaaggccgg atgcccccca ctgctgccac
     3301 tgtttttcaa atgccgtctg actcgcggga atgtcctgtt tctccacctt ccggtaatgt
     3361 tgtcgcaatt tttccatttg ctggctcagc gactggattt ttccctgttc gctacggtat
     3421 tccggtgccg cccattgcat caggggaata cgggaaaaac cggacgaaaa cggcgatgat
     3481 tttttcaggc tggcagcatg gctcatcgct tcctttcgtt cggtgttcag ctcttcccag
     3541 cgtctgcgcg tcgcctgcat atctgccatc atcgacatgg cattactggt gagtttccat
     3601 gccgcattac tgtccttcgg caacgcttcg atgatgtcac gtagtgtgta cgaagttgcg
     3661 ttgtcaggaa gcacgatatt cagaaggtca gccacacggc gggcaggctc tgtcagcccg
     3721 ctgtttctga gttcggtacg ggaaatctgt atttccaggt taagtctgtt aatcagacga
     3781 ttctgttcgc cgcgttctgt ttcgataccg cgtgtttcca tcgcacaggc agactttccg
     3841 aggtgaatac caggcgtttt ctcaagcccc tgtcgctcgt atgaacgatg gtctatctct
     3901 tcctgataac cggcgttcgc cagtgccctg ttcgcatggt cagcccatga tgcacgccac
     3961 gtctcggcat gtgtccggtc gttccagtcc ctgacctttc caccgaatcc agcaggtccg
     4021 acagctctcg tggtcagcat gatgtgcgca tggggatttg ttttatccat gtgatggaac
     4081 gccacatcag caatcatgcc ctgactgaca aagttttctc tgacaaaggc gagaacggtt
     4141 tcccgtgcgg catcctgcgg tagttcgcgg gggatggcga gttcaatttc ccgcgcaagt
     4201 tgtgagttct tccggcgttc ggccttctca acggcattcc agagttcggc acggttcgta
     4261 caccaggcag gggctgtcgc gggagtcaga atcacagctt cagccacacc gcttttgcgg
     4321 gtgtagtcgt gagtcagtcc ggtacgctca tcaagcatca gtgaaccgga acgataggca
     4381 gcagaagcaa cggcgctgta gccgctgttt ctggagatga ttttcatgct gagatgataa
     4441 atcgccattt ctgccgtaat gcctttttgt ggttttcttt cagccccgca gggcgcacac
     4501 aatcgaaacg gagtggagat tgtataagtg cgcacttcgt gattttccgt gcattatgcc
     4561 agctcagttt ttaaactacc agtgacgcgc taaaatgcga tttttcatcg cgcacggagt
     4621 tacatcatga gcagaacact cgaacagaag attgctgatg ctgaggccag attacagcgc
     4681 ctgaaggcga agagcaggag tctggacaca gcgcagaagg tcattgtggg tgcagcattg
     4741 ctggcaaagg tcagaaagcc ggaggaggtg cagttgcgtg cctggttact ccagttcctg
     4801 aaggcagagg tgacacgaca ggctgatgtg acgcgcatac tgccgctgat taacgagctg
     4861 gaagcgctgc caggacagtg attgtatcgt ccgctcgccg cagtcccagc gaccgagcgt
     4921 tagcgagcga gtgggcgagg aagcggaagt gaatccgtcg cctgattgag cacatacgca
     4981 cggtttttcg ggaggtgata tgcgctgaac ccgcatgaat actggatttc cacagggttg
     5041 gagggggtgt attcggaact tgcaattctg cttttttgca acgcattgtt tttattggtt
     5101 ttttgttttc agcactccaa gttatccaca ggctcgtcgt taagttttta gatcttgttt
     5161 ttaagatctt gtttttaata cttgttttag cgccaaaatt ccttatagaa aacaaaatgt
     5221 tacaacgcta taggtaatat gatttaaggt aaaggtaata tgatttaagg tgcttgggta
     5281 atatgattta aggtaaaggc aatatgattt aaggtaatat tcacagaaaa agggatatta
     5341 aaataaaaag gcattgcctt tcgttgaggg tatgtttatc ttttattcat taccttttgg
     5401 ggagggatgc tctatgtcgg atctaattgc ctataaatcc aatgcgctgg ttgaagccag
     5461 ttataaattg accctacagg aacagcgatt tttgttactt tgcatcggtc gattgaagtc
     5521 tggggctgat gcagaatctc caaaattgca aaagacaatg accattacag ctgcggaata
     5581 tttcgattca ttccctgaca tggggcggaa aaatgccgag gttcagttac aggaagcaat
     5641 tgatcgtttg tgggacaggt caatcattct caaggatgac gagaaacgtg aggagttccg
     5701 ctggatccag tatcgggcac agtacgctaa aggcgaggcc agagcacaaa taacattttc
     5761 tgacgcagta atgccttatt tgacacaact acaagggcaa tttactagag ttgtaatcaa
     5821 gaatatatct agattatcaa gaagttacag tataaggata tacgaactcc tccagcagtt
     5881 ccgcagtact ggcgaacgta ttatcactct cgatgatttt cgctcaatgc ttgatataga
     5941 gcataaatat caaacatata aatcacttaa ccagcaatta ttaagaccct gcattgacga
     6001 actgaacaaa aaaagcgacc tggctgtaac ggttgagacg atcaaaaagg ggcgcacggt
     6061 tgtagccctg cattttcgct tcaaggaaga caaacaaatc aagatgacga tctaaaatcg
     6121 attttaaggc acctaaaacg ctccctgccg cgtttttttt gtgttagtag tgcgaaggta
     6181 ctattgaagt gaaaaattgc gtcaaaagcg ctctaatgca gcgaaaaaag aacccccatt
     6241 tttgggggcg gaaaaacaaa actatgcttg cattatgtgt gtgcttcttg cattcatccc
     6301 tgtcgatgaa catcgaagaa catcgacgaa cataacacga tgttaaacaa aacaaaatat
     6361 tgttcacatt cactgtgacc aaccgcgatc atgtctcctc tcggtggcgt gtgttcgtgc
     6421 atcttcctcg cgccgccatt cgtcccaggt acgattaaaa cccagcgagc ggaacagatg
     6481 atccggctgt ttttctggtg tgttgacggc agcaaaaagc ggtgcctcac gggttttcag
     6541 atgaaactcc ataagttccg gcgcaggtga tatgtggctg tctgcatcgt gatttacggc
     6601 aacaccatac cctgcatacc gtaacccctt atcggtcagt gcactgatga ccagctggct
     6661 gttatcggct gagggaacgg caaacgcggg atacagcgta ccattcagcc ggacaacacc
     6721 gcccagagag tcatccacgc ccatgaccag caaaagctga agctggcggg tttcccggtc
     6781 gacagaactc attaccggca tcagcgtcgg tagcagacgt ccctgcgtaa aatccacctt
     6841 ccttgctgcc agtgatggca tggctggctg gcggtcttct ccaagacaga aagactccgg
     6901 ccatgtcttc ggctggcgga gattgcgggt atattgcggc catgtctggc aaacctgcgt
     6961 cagaagttgc tcaaaggtcg cagaggaata aacacgttgc agtggtggat gttgaccgat
     7021 ttcgcttcgg tcacagatat aaccactcct gtcacgcatt aaatcaacac gctcaaagat
     7081 tctggtgcgg tatttctcca gctcgttccc ccagagcgtg tattctccgt tccggttgtg
     7141 aagcagaagg taacaggtca tttccccgtt accaggcatg ggggcctcgc cataccccgt
     7201 aacgactgcg ctcagtaccc ctgaatccat aacggcattg tcatttttcc tgagctgttc
     7261 cgctcgtcgc tccgcgcgac tacgttctct atcctcctgc tccttccggc gcaattcctc
     7321 ttcacgttta agcaactgga ctctgagagc tgcaaggccg gatgcccccc actgctgcca
     7381 ctgtttttca aatgccgtct gactcgcggg aatgtcctgt ttctccacct tccggtaatg
     7441 ttgtcgcaat ttttccattt gctggctcag cgactggatt tttccctgtt cgctacggta
     7501 ttccggtgcc gcccattgca tcaggggaat acgggaaaaa ccggacgaaa acggcgatga
     7561 ttttttcagg ctggcagcat ggctcatcgc ttcctttcgt tcggtgttca gctcttccca
     7621 gcgtctgcgc gtcgcctgca tatctgccat catcgacatg gcattactgg tgagtttcca
     7681 tgccgcatta ctgtccttcg gcaacgcttc gatgatgtca cgtagtgtgt acgaagttgc
     7741 gttgtcagga agcacgatat tcagaaggtc agccacacgg cgggcaggct ctgtcagccc
     7801 gctgtttctg agttcggtac gggaaatctg tatttccagg ttaagtctgt taatcagacg
     7861 attctgttcg ccgcgttctg tttcgatacc gcgtgtttcc atcgcacagg cagactttcc
     7921 gaggtgaata ccaggcgttt tctcaagccc ctgtcgctcg tatgaacgat ggtctatctc
     7981 ttcctgataa ccggcgttcg ccagtgccct gttcgcatgg tcagcccatg atgcacgcca
     8041 cgtctcggca tgtgtccggt cgttccagtc cctgaccttt ccaccgaatc cagcaggtcc
     8101 gacagctctc gtggtcagca tgatgtgcgc atggggattt gttttatcca tgtgatggaa
     8161 cg
//
