LOCUS       NZ_CP147047             2676 bp    DNA     circular CON 22-APR-2024
DEFINITION  Escherichia coli strain 19A14 plasmid p19A14_1, complete sequence.
ACCESSION   NZ_CP147047
VERSION     NZ_CP147047.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39992805
            Assembly: GCF_038396545.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Gomi,R.
  TITLE     Genomic analysis of environmental Escherichia coli carrying
            transferable quinolone resistance determinants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Gomi,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2024) Department of environmental engineering,
            Kyoto University, KyotoDaigakuKatsura, Nishikyo-ku, Kyoto-city
            615-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038396545.1-RS_2024_04_20
            Annotation Date                   :: 04/20/2024 18:36:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,639
            CDSs (total)                      :: 4,516
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 35 of 97
            Pseudo Genes (incomplete)         :: 62 of 97
            Pseudo Genes (internal stop)      :: 19 of 97
            Pseudo Genes (multiple problems)  :: 18 of 97
            Pseudo Genes (short protein)      :: 3 of 97
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147047.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="19A14"
                     /isolation_source="municipal wastewater"
                     /db_xref="taxon:562"
                     /plasmid="p19A14_1"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
                     /collected_by="Fumie Adachi"
     gene            125..286
                     /locus_tag="WAZ55_RS23175"
                     /old_locus_tag="WAZ55_23175"
     CDS             125..286
                     /locus_tag="WAZ55_RS23175"
                     /old_locus_tag="WAZ55_23175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071527578.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHDLSPLAAATGNRIRGVSLE
                     VMRLLRLN"
                     /name="replication initiation protein"
     gene            complement(498..902)
                     /locus_tag="WAZ55_RS23180"
                     /old_locus_tag="WAZ55_23180"
     CDS             complement(498..902)
                     /locus_tag="WAZ55_RS23180"
                     /old_locus_tag="WAZ55_23180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000014067.1"
                     /translation="MSGGNNFKMSQDYEIAQPKKKMAYPILVEEWDFLIGKIESISDDE
                     NIYHTIGSIFLGVAGSALIAALTLDLPSDDGYMPRQILISWYVFWASLICGVLSMFFGR
                     KNKKINRTSAKDIVQQMRLIEKRYEQGSMD"
                     /name="hypothetical protein"
     gene            complement(899..1171)
                     /locus_tag="WAZ55_RS23185"
                     /old_locus_tag="WAZ55_23185"
     CDS             complement(899..1171)
                     /locus_tag="WAZ55_RS23185"
                     /old_locus_tag="WAZ55_23185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001286144.1"
                     /translation="MSDVKSDQIIKYLSEKWGGSKCPLCKKGRWNVSDKIFELREFHEG
                     NVVIGAGPIVPIIPVTCDNCGNVVLVNAIISGCLSADNKQDGGSK"
                     /name="hypothetical protein"
     gene            complement(1530..1853)
                     /gene="mobA"
                     /locus_tag="WAZ55_RS23190"
                     /old_locus_tag="WAZ55_23190"
     CDS             complement(1530..1853)
                     /gene="mobA"
                     /locus_tag="WAZ55_RS23190"
                     /old_locus_tag="WAZ55_23190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            2137..2331
                     /locus_tag="WAZ55_RS23195"
                     /old_locus_tag="WAZ55_23195"
     CDS             2137..2331
                     /locus_tag="WAZ55_RS23195"
                     /old_locus_tag="WAZ55_23195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041622.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALNADEQAAMCEKLHELA
                     EELQNSIQTRFEAENLTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(1863..2099)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.49e-121"
     Typing          2..445
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.333"
                     /coverage="98.0"
                     /evalue="8.43e-111"
     Typing          complement(1534..1853)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="9.47e-63"
ORIGIN
        1 ctcgtgcgct ctcctgttcc tgcctttcgg tttaccaatg tcattccgca gttatggccg
       61 cgtttatctc attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga
      121 ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta actatcgact
      181 tgaatccaac ccggaaagac acgacttatc gccactggca gcagccactg gtaaccgaat
      241 tagaggagtt agccttgaag tcatgcgcct gttaaggcta aactgaaaga acaagttttg
      301 gtgactgcgc tcctccaagc cagttacctc ggctcaaaga gttggtagct ctgagaacct
      361 tcgaaaaacc accttgccgg gtggtttttt cgtttcatag acaagagact acgacgacaa
      421 gacaaggatc tcaagaagat ccttacatta tgccccccct tggcacgccg taacctcgct
      481 aaacgttcag catccagtta atccatacta ccttgctcgt aacgtttctc tatcaatctc
      541 atttgttgca caatatcctt cgcagaagtt ctattaatct ttttattctt tctaccaaaa
      601 aacatagaaa gcactccaca aataaggctt gcccaaaata cgtaccatga aattaatatc
      661 tgccttggca tataaccatc atctgatggc aaatctaaag ttaatgctgc tatcaatgcc
      721 gagccagcca cacctaaaaa tatagaacca atcgtatggt atatattttc atcatcacta
      781 atactttcta ttttccctat taaaaaatcc cattcttcaa caagaatagg gtaagccatt
      841 ttcttttttg gttgagcaat ttcatagtcc tgagacattt taaaattatt accaccactc
      901 actttgagcc tccatcttgc ttattgtcag cagataagca ccctgagata atggcattaa
      961 caagaacaac attcccgcaa ttatcacaag ttacaggaat aattgggact atcggacctg
     1021 ccccaataac aacattacct tcatgaaatt ctcttagttc aaaaattttg tcagaaacat
     1081 tccaacgccc ttttttacac aagggacatt tgctccctcc ccacttttca gacaaatatt
     1141 taattatttg atctgactta acatcactca tattccctcc aagcattcct tatactcata
     1201 tatttattag cgaacaatca catcactcaa taatttattg atcaagtaac tttatagagt
     1261 acatccatat ctgttttgtg cgcaaaaaaa cataaatata aaatcttaca tttaccataa
     1321 taccagtatt gtacgcaccg ctgaaacgcg ctgaacgcga ttctgacgca gacaccgcaa
     1381 aaagagatgt tttaccccgg gaaaacagga aggatgcgtc aggatcgttt tcagctcgct
     1441 gcatagctat gcatgaaagc gaatggtgat cactttggga gcttacggtg ttcataccgt
     1501 ctgttttcga cagttttctc tccgggaagc taatctgcca taagtctgga taacagggca
     1561 cggtgatact ccgtaatagc gatcagcacc tccgcatact ccctgtcccc gacacgcttg
     1621 ccgtcgataa agagtttttt ctgtaacgcc cccagtctca taacttcttt cagtacccga
     1681 tcatcagtca gtgagttaac tttcttaccg agagctgccg cccgtaaaaa accggaaacg
     1741 gtcttgccgc attcagcagc cctttttctg atttcctggt cttcttctgc cgtcaggcga
     1801 acagggcgac tgatagcccg cctgcgcgta ttacttccac ttcgttttgt cataaaacat
     1861 ggctccgtat ctgacatggg tgtcggggca aagccctgac cagggcaatt gtaatagcgt
     1921 gcatgtatgc gcggtataac aattgcacat cctgtcctgt cagcaagctc gaattaccga
     1981 caaaggcacg gcagtttttt ggcagtgaca gtgttgtctg acagaaatgc ccgtcaaaaa
     2041 gccaagcggc agaatagcgg cagcaaaaca gtcgaaaagt ggtacgcgat ataatcacac
     2101 aacgcagatg acagacagaa cggagaaaac gaaaagatga acaagcagca gcaagccgta
     2161 ctcaacatgg cgggattcat aaaaagtcag agcctgacgc tgctcgaaaa actggacgca
     2221 ctcaatgccg acgaacaggc cgccatgtgt gagaaactgc acgaactcgc agaagaactc
     2281 cagaacagca tacagacacg ctttgaagcg gaaaatctta ccgggatata acgctccaga
     2341 caggatgcag aacggcatag aggagcgtaa ggagaaaatg tcacataagg cgctcttccg
     2401 cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gtgtcagctc
     2461 actcaaaggc ggtaatacgg ttatccacag aatcggggga taaaaccgga aagaacatgt
     2521 gagcaaaaga caaagactca gaagaagggc gcgcgggagg cgtttttcca taggctccgc
     2581 ccccctgacg agcatcataa aaaccgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2641 ctataaagat accaggcgtt tccccctgga agctcc
//
