LOCUS       NZ_CP147037            33826 bp    DNA     circular CON 22-APR-2024
DEFINITION  Escherichia coli strain 21F61 plasmid p21F61_3, complete sequence.
ACCESSION   NZ_CP147037
VERSION     NZ_CP147037.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39992807
            Assembly: GCF_038396565.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33826)
  AUTHORS   Gomi,R.
  TITLE     Genomic analysis of environmental Escherichia coli carrying
            transferable quinolone resistance determinants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 33826)
  AUTHORS   Gomi,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2024) Department of environmental engineering,
            Kyoto University, KyotoDaigakuKatsura, Nishikyo-ku, Kyoto-city
            615-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038396565.1-RS_2024_04_20
            Annotation Date                   :: 04/20/2024 18:26:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,562
            CDSs (with protein)               :: 4,562
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 73 of 178
            Pseudo Genes (incomplete)         :: 128 of 178
            Pseudo Genes (internal stop)      :: 31 of 178
            Pseudo Genes (multiple problems)  :: 48 of 178
            Pseudo Genes (short protein)      :: 4 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP147037.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..33826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="21F61"
                     /isolation_source="river water"
                     /db_xref="taxon:562"
                     /plasmid="p21F61_3"
                     /geo_loc_name="Japan"
                     /collection_date="2021"
                     /collected_by="Fumie Adachi"
     gene            complement(join(33310..33826,1..392))
                     /gene="virB9"
                     /locus_tag="WAZ52_RS00005"
                     /old_locus_tag="WAZ52_00005"
     CDS             complement(join(33310..33826,1..392))
                     /gene="virB9"
                     /locus_tag="WAZ52_RS00005"
                     /old_locus_tag="WAZ52_00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_001299943.1"
                     /translation="MMKKLFIATCLLLPGLVYSAATPLPSGFDARMQTVSYNGANTTVI
                     RSKTGFLTSVVFDEGEAVISAKAGFPAGWEITTDDNVVYINPRPVVQEQEGDEGEKLKK
                     VFQPTEKEWDTNLFVRTTKRIYSLDLILLSEEKQAQPAYVVQFRYPSEIAKKNAEEVRL
                     AKEKQEKLRQKKLISESFEKADAPKNWDYFMRVNEKYDSRRIAPDFAYDNGIFTFLGFN
                     SGKVFPAPFAVRDGQEQTLAFNVETKGKYKIMVIHNVNDKFVLRYGNSVVGVVNKSFGK
                     VLTDQRNTSSPAVERVEVNND"
                     /name="virB9"
     gene            complement(389..1075)
                     /locus_tag="WAZ52_RS00010"
                     /old_locus_tag="WAZ52_00010"
     CDS             complement(389..1075)
                     /locus_tag="WAZ52_RS00010"
                     /old_locus_tag="WAZ52_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_001290765.1"
                     /translation="MSEKIEKKIETAKSFERQLYAENERSKKNAWRVAVAASLLSLCLG
                     AAICVLVPLKEKELAIVAVGEKTGRTELITQVKQEKILQSEALGRYFVNTYITLREGYN
                     YPSLQYDYETVQLYSSNTVKDDYLRLYNSDMAPDKIYHNNGSYVAVEVISNIISDATAP
                     DKLASVRFKKTTRNFTTGQVSVSYWTARVTYRFEPEKSVKSSSRELNPLGFTVTSYQTD
                     REVRGE"
                     /name="type IV secretion system protein"
     gene            complement(1590..2684)
                     /locus_tag="WAZ52_RS00015"
                     /old_locus_tag="WAZ52_00015"
     CDS             complement(1590..2684)
                     /locus_tag="WAZ52_RS00015"
                     /old_locus_tag="WAZ52_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677777.1"
                     /GO_process="GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000046889.1"
                     /translation="MAQGFFVKYNSTVMDSVDKISSSYQTQFANDIMSLATVSVTLYVL
                     WKGYQILASKTQTPLQDLVWDLSKFAIIIMFITNADGYLTAATDALQGMKDGFSGGVSV
                     WQTLDNLWKSTQNLGAEIYSLDKSTYVKDQGVVGQFLIWTGSLILMAVSVVVFLTADVT
                     MKLLIITAPIFIFCLMFGFIRSMFNNWLQSLFSSILTVLFASLVIRIAMDFQGMILSHA
                     IRAAQTGNVNLVSTGAMGFMAGFLGALLVLIAKGFAVQLAGAGVEGAVQGAAMMGLGAA
                     GMATGKSLMLGGRAGLGFGLGMAGRTGMNSLSGKAGNLMGRGARTAAEWAGEKGYQALA
                     PTGALGMKARRLASLEKARARNAA"
                     /name="type IV secretion system protein"
     gene            complement(2696..3412)
                     /locus_tag="WAZ52_RS00020"
                     /old_locus_tag="WAZ52_00020"
     CDS             complement(2696..3412)
                     /locus_tag="WAZ52_RS00020"
                     /old_locus_tag="WAZ52_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000832280.1"
                     /translation="MKRVKTFMLISLLTASFYSSAGIPVAIDASPEWAVEAARWTERLK
                     QWSETAQHYQSQIQAYKDQLATATGIRNIAAFTNELSNLQSELTNIYKQGNSYISDFTS
                     NPEGALSSQAKNLFSKYGAFDMCNTGYERNDNLCKARIVSTAASIEQGNEINKQLSNAM
                     SQIQSLSSRIEASKDIKESQDLANALQAQSLKMQAIKMQYDVWNNKNKADHEMLITQEQ
                     EAFIKQQKEAEPLTFD"
                     /name="type IV secretion system protein"
     gene            complement(3424..6171)
                     /locus_tag="WAZ52_RS00025"
                     /old_locus_tag="WAZ52_00025"
     CDS             complement(3424..6171)
                     /locus_tag="WAZ52_RS00025"
                     /old_locus_tag="WAZ52_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="WP_000105985.1"
                     /translation="MSTLYKAMTRPAMYVGVPVVPLTVVAGALFLAGVYISKLIWLAIP
                     VAVFVLRMITKQDDHIFNLYFLKLKMLGNSVCNRFFGARAFLSGQYEAVEIDEFVNAMK
                     LNERITTGKYIPYSSHVDKNIVKTKNGDYVATWQLMGINFESISAEMLETIDSQVATLV
                     RSFSGLPVSFYNHSCRASFYDAFTTKSGNKYADIISDCYYGSMKKNKFKGNTLYFTLIY
                     RPDGRVEKLEKRKKSIKEKKDDINIHVKRMNEMINTFSGALDKFTCKLLGMYEENGKVF
                     SSQLSFYNYLLTGKLQKIRVTDSPVYNVLGGVDVFFNHDTGQICRIDGNKFFRSIEIKD
                     FCSETASGVFDVLQYSDADYIITHSYTSMSKSEALSTIKRAEKQLKSTEDDAVTQLQEL
                     EKAKNDIVSGDISFGYYHFTLMVMADSIRELDESVSKITADFTDLGIIPALSTMSLPAA
                     YFAQLPAVFHLRPRLSPVSNVNFVELASFHNFYQGKRDKNCWTEAVAILKTPSKQAYYL
                     NLHNSVLFKDETGEKNLANTKVIGTAGSGKTMFLSYLACSLQKYNNPETFADSAKNKKL
                     TCVFLDKDRGAELCIRMLGGEYYTVKSGEPTGWNPFALEATKRNRIFVKQLMEILCTRN
                     GERLSTRERLLISESVDAVMDFPPGEMREYGITRMLEHLMQRDDRDEQENGIILRLSQW
                     ANGQAHGWVFDNAKDTFNIQHVNNFGIDGTEFLDDPMVCAPITFYLLYRITQLLDGRRL
                     VIFLDEFWKWLQDEAFSDFVYNKLKTIRKLNGLVIPATQSPDEILKNKISRAVVEVCST
                     SIYLANPDADYNDYVEGLKLTPEEFNIVKNLDPMSRQFLIKKSSLKKGDGKSFSALATL
                     DLSGLGGYLKILSASADNLEIFESIYHEGMEPDDWVPEYLERAI"
                     /name="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
     gene            complement(6190..6516)
                     /locus_tag="WAZ52_RS00030"
                     /old_locus_tag="WAZ52_00030"
     CDS             complement(6190..6516)
                     /locus_tag="WAZ52_RS00030"
                     /old_locus_tag="WAZ52_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_000008647.1"
                     /translation="MSFKKSVCNRISSSVTAVMQRKNKVVMAVAGATVASPAFADGFSK
                     AETLLEKVKTGLSGLSLVTVTIACLWVGYKVLFGGSTIRECSPIIIGAIVIASAAEVAS
                     MMVN"
                     /name="TrbC/VirB2 family protein"
     gene            complement(6530..7150)
                     /locus_tag="WAZ52_RS00035"
                     /old_locus_tag="WAZ52_00035"
     CDS             complement(6530..7150)
                     /locus_tag="WAZ52_RS00035"
                     /old_locus_tag="WAZ52_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_001299949.1"
                     /translation="MASLFPALLLCAASVHPDTINDIARVESGFNPYAVAEIIPERERG
                     SYGKSVISHMPKSKEEALTVIREIENRKRRYSVGLMQITNSNFSFYSTSAEKLLDPCEN
                     LSVFEKIIVDCYKRGRSLENALSCYYTGNFSNGKRKEKEFNNTSYVERIGYTGNEKKYV
                     VPGTRSNGGEQRKNRSHNASVIWPETILKSAFVDNSHPTKVIN"
                     /name="lytic transglycosylase domain-containing protein"
     gene            complement(7153..7329)
                     /locus_tag="WAZ52_RS00040"
                     /old_locus_tag="WAZ52_00040"
     CDS             complement(7153..7329)
                     /locus_tag="WAZ52_RS00040"
                     /old_locus_tag="WAZ52_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000660496.1"
                     /translation="MKEKPDKSSEKGVYVTIKLDPRLNAFIEEKTKTSLLSKRKQIIYL
                     LMQSMRIDGYMER"
                     /name="hypothetical protein"
     gene            complement(7506..7721)
                     /locus_tag="WAZ52_RS00045"
                     /old_locus_tag="WAZ52_00045"
     CDS             complement(7506..7721)
                     /locus_tag="WAZ52_RS00045"
                     /old_locus_tag="WAZ52_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000760375.1"
                     /translation="MKKYLLFALPFFVVGCSEEVKSVDWWGQHLTEAKQKQAECEKSGS
                     DSQNCKNVKQALFIQSQKDAPVPTFD"
                     /name="EexN family lipoprotein"
     gene            complement(7718..8011)
                     /locus_tag="WAZ52_RS00050"
                     /old_locus_tag="WAZ52_00050"
     CDS             complement(7718..8011)
                     /locus_tag="WAZ52_RS00050"
                     /old_locus_tag="WAZ52_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134733.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]; GO:0016987 - sigma factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="WP_000134733.1"
                     /translation="MTEHDAICISALHQIFSDEEHLSEQQKDIILMYAYGYTLNEIADF
                     KGLKPSTVRKYLDSVRAELGGVSLAGIRTLVLIRTNALLVSSLSRISERGNL"
                     /name="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            complement(8088..9317)
                     /gene="taxC"
                     /locus_tag="WAZ52_RS00055"
                     /old_locus_tag="WAZ52_00055"
     CDS             complement(8088..9317)
                     /gene="taxC"
                     /locus_tag="WAZ52_RS00055"
                     /old_locus_tag="WAZ52_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase/nickase TaxC"
                     /protein_id="WP_000539545.1"
                     /translation="MGVYVEQEYRIKRAKGTGRDPKSPKLSGRHIHASKSAFNHKVRHG
                     TDKKAYTWTPTKEVTFKITGSGKTAAGIKNGIDYITRNGELEAYCYDGKGSEQTGKGED
                     FNREFTSTLSKGNDYSRTYRGENIDHVKNMVFSPPPEAGVSREDALKATIEFLKETYPN
                     HAFVAVYHDDKEDHPHVHVNIKLRDEETGRRLRLTKSETRKFRNGFHRKLKGMGYDVTA
                     TWKKDPERKREIERLQAENPKRLRNVYKVVDFGETSYQNKAGEKRTPFLTYETLKGGKQ
                     VTIWGKDLKNHFESEKLQPGTLIKVKKLAPTLVRSPMFNDDGTVAGYRETHRNNWQIEN
                     IALERNRERQVHERETRQPEESDVKKQLSRKHEQGHNIGFALEHGFTKDSEEHKKLRIQ
                     QERNWKGLGF"
                     /name="taxC"
     gene            complement(9321..9824)
                     /gene="ddp1"
                     /locus_tag="WAZ52_RS00060"
                     /old_locus_tag="WAZ52_00060"
     CDS             complement(9321..9824)
                     /gene="ddp1"
                     /locus_tag="WAZ52_RS00060"
                     /old_locus_tag="WAZ52_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 1"
                     /protein_id="WP_000781818.1"
                     /translation="MKMIAFRLSDDELKLAEHNAISSGFTSINTFARHNVLNVDVQPSN
                     IPVSNESSKAVKTCLYPHEIELVKRNAALHGMSMSREIAIRVRQSLLKNEVCLYPDEVK
                     ELKKLSTAVDRVGRNIHFIIKGERFCTVNDPDFRKDVVEVIELCKQIDSKLETLTKSVV
                     NRFG"
                     /name="ddp1"
     gene            complement(10187..>10384)
                     /locus_tag="WAZ52_RS00065"
                     /old_locus_tag="WAZ52_00065"
                     /pseudo=""
     CDS             complement(10187..>10384)
                     /locus_tag="WAZ52_RS00065"
                     /old_locus_tag="WAZ52_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /name="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            complement(10635..11000)
                     /locus_tag="WAZ52_RS00070"
                     /old_locus_tag="WAZ52_00070"
     CDS             complement(10635..11000)
                     /locus_tag="WAZ52_RS00070"
                     /old_locus_tag="WAZ52_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000854262.1"
                     /translation="MKTLKQAAMQFASDLRNNRCFKAAYNDALLELDREDLNAIVQCTF
                     LFEPKRTLAELDKLIKKIEVEEDYEFCVSLDKGNGLHEVRTFKSEREALDAYRILTLYG
                     YTVTIEKKEGNDWLFKV"
                     /name="hypothetical protein"
     gene            complement(11092..11391)
                     /locus_tag="WAZ52_RS00075"
                     /old_locus_tag="WAZ52_00075"
     CDS             complement(11092..11391)
                     /locus_tag="WAZ52_RS00075"
                     /old_locus_tag="WAZ52_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000650313.1"
                     /translation="MKCSSVFTSTTNHVFTFERVTLCTIILMHKDTGQQYVVLFTDNNK
                     IRDYKTGIVPQFGELKQSDVDLVLFYRDEYEKYFDSLKDGDECLSFKDFIECLR"
                     /name="hypothetical protein"
     gene            complement(11783..12010)
                     /locus_tag="WAZ52_RS00080"
                     /old_locus_tag="WAZ52_00080"
     CDS             complement(11783..12010)
                     /locus_tag="WAZ52_RS00080"
                     /old_locus_tag="WAZ52_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000801440.1"
                     /translation="MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQN
                     SQSLSLVEFEFLLLLAGEHPEYVLCKRESK"
                     /name="hypothetical protein"
     gene            complement(11997..12248)
                     /locus_tag="WAZ52_RS00085"
                     /old_locus_tag="WAZ52_00085"
     CDS             complement(11997..12248)
                     /locus_tag="WAZ52_RS00085"
                     /old_locus_tag="WAZ52_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001230708.1"
                     /translation="MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDA
                     VLNENELQHIDFTCAGLRKDDETPVKNLIKEFMLNETR"
                     /name="hypothetical protein"
     gene            complement(12245..12787)
                     /locus_tag="WAZ52_RS00090"
                     /old_locus_tag="WAZ52_00090"
     CDS             complement(12245..12787)
                     /locus_tag="WAZ52_RS00090"
                     /old_locus_tag="WAZ52_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001083908.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCSSRIKLTSPQFKNY
                     SVHIRMEKDRFSVVGSVDSRSWRSPHHVCTLSRKRNPVDIAADIERKILVNASQEVLQA
                     IEYEKHQVEKKDEIMILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGDSYDLQIR
                     GLSIDQLVKITGYLKQL"
                     /name="hypothetical protein"
     gene            complement(12892..13407)
                     /locus_tag="WAZ52_RS00095"
                     /old_locus_tag="WAZ52_00095"
     CDS             complement(12892..13407)
                     /locus_tag="WAZ52_RS00095"
                     /old_locus_tag="WAZ52_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025396.1"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_001025396.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELMK
                     AVNAAFDVLMANIDKINQFQSTDEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVWIS
                     GETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRRATG
                     WQRVETRV"
                     /name="J domain-containing protein"
     gene            complement(14046..14396)
                     /locus_tag="WAZ52_RS00100"
                     /old_locus_tag="WAZ52_00100"
     CDS             complement(14046..14396)
                     /locus_tag="WAZ52_RS00100"
                     /old_locus_tag="WAZ52_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000127483.1"
                     /translation="MTATKNVKSQKNDYSASVDHNAAMIEELRADPDYANAYLANALEE
                     INEPGGLGGFLVALRQVIEARGGISEAARKSGLARQSIYRALSPNGNPTITTLAQLTSV
                     AGLQFTLSKSSH"
                     /name="hypothetical protein"
     gene            complement(14393..14728)
                     /locus_tag="WAZ52_RS00105"
                     /old_locus_tag="WAZ52_00105"
     CDS             complement(14393..14728)
                     /locus_tag="WAZ52_RS00105"
                     /old_locus_tag="WAZ52_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004026069.1"
                     /GO_function="GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001147119.1"
                     /translation="MPYTAKRYQTENGEVPYTDWMKKLRRKDQTAALKVDSRIARAMGG
                     NFGDHKFERDGVWELRVDYGPGYRVYYSIEDGEIILLLIGGNKKTQTADLDKAVSYLQD
                     FKKRSKK"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            16272..17279
                     /locus_tag="WAZ52_RS00110"
                     /old_locus_tag="WAZ52_00110"
     CDS             16272..17279
                     /locus_tag="WAZ52_RS00110"
                     /old_locus_tag="WAZ52_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243325.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_000243324.1"
                     /translation="MVLKNNKNSDCNDVQSLLAQGNQLLEGAYDITLIEMRLLYLALTK
                     IDSRKPQPANEYTLFAKEYRDTFSLDSKNCYEQLKSAASSLGSKPIVTYEWNEAKKRID
                     AVKRFWFSSIRYGVGNSESDITLRFSDSVSQYLYELKSEFTQMNLEHMVKLDTPFSFRL
                     YSWLYKYKNLNRNKKHSGVISTDPITIEWMKERTGLTGKYPVYKDFKKRVLDPAVDIIN
                     ANTNLSVTYEGIKSGRKIESVVFTYLVENETHDGKKIATKPLRPRLPSRPRVIKGSSAE
                     VAWAKKCIDVMKNYLISLREYDDKLKLSRPDLQKVKLWHEITGWDFEESFWFSD"
                     /name="replication initiation protein"
     gene            17345..17749
                     /locus_tag="WAZ52_RS00115"
                     /old_locus_tag="WAZ52_00115"
     CDS             17345..17749
                     /locus_tag="WAZ52_RS00115"
                     /old_locus_tag="WAZ52_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000654313.1"
                     /translation="MKDNIVDFQRIRRDLGAHSTDVANDAVYSDKKKTSRFFYLLTPVR
                     YLLAASLYLVVVIPLGILAGFRFLLQFLGGIAVMGLTIAWYMGADVSGYIVLAAWIALT
                     VCGCAEQIFEWWIESRFAFRLFGIGRTPEE"
                     /name="hypothetical protein"
     gene            17885..18412
                     /locus_tag="WAZ52_RS00120"
                     /old_locus_tag="WAZ52_00120"
     CDS             17885..18412
                     /locus_tag="WAZ52_RS00120"
                     /old_locus_tag="WAZ52_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="WP_000577047.1"
                     /translation="MIFNRNKKRDEQQNSSVVLPSTENVFSDFIKNNPALTTNAFTAIG
                     EVDCFTKVPLLSNKESEFLGILSRNIIPDYSVFPKIRLIEFVRPHGSEEAVKELIREVQ
                     NITCDFVLKSYDETVLAVIQFGETATVRQQKKKLIIQRVCAMTEISFFEFKNTVDMMGS
                     EDFCQFLRDDEE"
                     /name="DUF2726 domain-containing protein"
     gene            complement(18719..18934)
                     /locus_tag="WAZ52_RS00125"
                     /old_locus_tag="WAZ52_00125"
     CDS             complement(18719..18934)
                     /locus_tag="WAZ52_RS00125"
                     /old_locus_tag="WAZ52_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000399460.1"
                     /translation="MEHCIFDLGIINYHPVITWTVPVAFFFLVLLAGIVFNIGRKSNLI
                     VFILAMLLPVTTVFIFMLLGGKIDMC"
                     /name="hypothetical protein"
     gene            complement(18984..19235)
                     /locus_tag="WAZ52_RS00130"
                     /old_locus_tag="WAZ52_00130"
     CDS             complement(18984..19235)
                     /locus_tag="WAZ52_RS00130"
                     /old_locus_tag="WAZ52_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001482241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000905709.1"
                     /translation="MLIKIIKSVFNFYMLCVQPFIGLVLAGYGAWKFSGDSISALLEPA
                     SYFVIGTVVLFVWYIAWQKERSKEEEFKSSIKPEDFVK"
                     /name="hypothetical protein"
     gene            complement(19272..19574)
                     /locus_tag="WAZ52_RS00135"
                     /old_locus_tag="WAZ52_00135"
     CDS             complement(19272..19574)
                     /locus_tag="WAZ52_RS00135"
                     /old_locus_tag="WAZ52_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001062964.1"
                     /translation="MAKIPKKIDQEIKPLDLRQFVSSAAQKPASASAVTRINLTMTDDD
                     LDTAQVFQNKYGASRAEVIRAALAALRLVSAEERRRLFDDIRKNSPKAGRPTVNK"
                     /name="hypothetical protein"
     gene            complement(19740..20399)
                     /locus_tag="WAZ52_RS00140"
                     /old_locus_tag="WAZ52_00140"
     CDS             complement(19740..20399)
                     /locus_tag="WAZ52_RS00140"
                     /old_locus_tag="WAZ52_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000516230.1"
                     /translation="MGKIITVAGHKGGIGKSTVLCSLCVCVIKKGKTACFLETDSQGSI
                     KDFIEDRKTNARLSEIPYFECYTDIPAMAQKLAARFDYVFVDTPGMKSPAFVKALSCAH
                     ILFTFIEPGSGIEINTLGRLVFDIKTAQAGVNPSMKAWIVLNKCSTNPSDSEASELRRQ
                     LNDDPDWLPVPRQRIYMRTAHKKAYNNGMGVHEYDDKRGNKARGEIELLLKETDIL"
                     /name="division plane positioning ATPase MipZ"
     gene            complement(20504..21328)
                     /locus_tag="WAZ52_RS00145"
                     /old_locus_tag="WAZ52_00145"
     CDS             complement(20504..21328)
                     /locus_tag="WAZ52_RS00145"
                     /old_locus_tag="WAZ52_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487124.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_016238959.1"
                     /translation="MSDLPASIFQISRSDAWNLTISPDAARNMRDLASQIGAGMPKYLI
                     APEVSLLLSYIDDLNIRMYFETLWNTGARLNEALALKPADFMLTPTRQYPQPVVILKTL
                     KQRLKEAERRPGRPTTRHPPLHPDPRYRKPPEPAVRLVPVLDAGYALRMREYLATWKKR
                     IKHQPVWDITSRQTPLNWINTAVARAERDGVTFSVPVTPHTFRHSYAMHLTMSGVPPRV
                     LQSLLGHRYARSTEIYTRVFSLDVLTGSGLSFTCDPQLAKQLLGYPERLNNE"
                     /name="tyrosine-type recombinase/integrase"
     gene            complement(21413..21802)
                     /locus_tag="WAZ52_RS00150"
                     /old_locus_tag="WAZ52_00150"
     CDS             complement(21413..21802)
                     /locus_tag="WAZ52_RS00150"
                     /old_locus_tag="WAZ52_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236115859.1"
                     /translation="MTLSKILTDIFRTANNLTHCKTTNQLLAGDASSTLASAILSCDRY
                     SIFEQDYPRSSLIRQAVRHTGHSVQTSLVTTFKYSVPLSGALFRHMCPYPGLFRITASF
                     SPCPVVHIHLDKNALSEKMSKFFFW"
                     /name="hypothetical protein"
     gene            complement(21885..22541)
                     /locus_tag="WAZ52_RS00155"
                     /old_locus_tag="WAZ52_00155"
     CDS             complement(21885..22541)
                     /locus_tag="WAZ52_RS00155"
                     /old_locus_tag="WAZ52_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001478769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000972911.1"
                     /translation="MMIKSKKVVVIFVALSSFLLAGCDSPKDANKQNFEKAINTRLEKE
                     CITISPLGMFDSKPYPVQIAVAQANRYTSQERADEINSRQFSALDVLVKADLLTVKNTL
                     VDDVIGFTKTGKKVPGREYALTDEGKKYLKSPERPDFCVGHYKVDEIVDFTEPGDAMGM
                     KITQVNYTFSPTSIAEWAKRDDVRAAFLGLESDLKEKQTKHITLVLKNDGWSAER"
                     /name="hypothetical protein"
     gene            complement(22645..23109)
                     /locus_tag="WAZ52_RS00160"
                     /old_locus_tag="WAZ52_00160"
     CDS             complement(22645..23109)
                     /locus_tag="WAZ52_RS00160"
                     /old_locus_tag="WAZ52_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293133.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0030527 - structural constituent of chromatin [Evidence
                     IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="WP_001293133.1"
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREIDFEQLLEMQEKLNVV
                     IEERREDAEREAAERQELEAKRQQAIEYIISLGLDPESLLAPVTADTVKTRRKAKGGVR
                     KAKYRFKDENGEIREWSGNGKRPLALQKLLDEGHFMEDFLIDKLQPGQVE"
                     /name="H-NS family nucleoid-associated regulatory protein"
     gene            complement(23125..23331)
                     /gene="hha"
                     /locus_tag="WAZ52_RS00165"
                     /old_locus_tag="WAZ52_00165"
     CDS             complement(23125..23331)
                     /gene="hha"
                     /locus_tag="WAZ52_RS00165"
                     /old_locus_tag="WAZ52_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="WP_000850859.1"
                     /translation="MKTKQEWLFQLRKCTSRDTLEKVIEINRYKLPLSESEAFYSAADH
                     RRAELVMNKLYDKVPSGVWKYVH"
                     /name="hha"
     gene            complement(23328..25481)
                     /locus_tag="WAZ52_RS00170"
                     /old_locus_tag="WAZ52_00170"
     CDS             complement(23328..25481)
                     /locus_tag="WAZ52_RS00170"
                     /old_locus_tag="WAZ52_00170"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="WP_001235747.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNAIVTNCFGHII
                     ESQPPENYNPEYKAWKVETLPLRLYPVKYQPVESAAKQVKTILELIRRGDVTEIVHAGD
                     PDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKALARS
                     VADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFYYTMT
                     GVFQRGADVIRANWKPGEFAPLTDRKLLDKAWADGTAASLAGKPATVEAAATDDKKTAA
                     PLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQFSEAPQV
                     IDALKSVFPQSLDIDSARKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERNVYLAIAQ
                     HYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGESEDNDDSA
                     FELLCKIRTGETLTTKEVVVNEKKTTPPPLFTEASLLAALVRVADFVTDPTIKKLLKDK
                     DKDKKDEHGGIGTPATRAAILETLKKRNYITLEKGKLIPTDTGYALIDALPGIAVNPDM
                     TALWSEKQTAIENGDLTVEEFINELYGELTGMISDVDLGEMKIEPAAPAGQFQRLDSPC
                     PSCGKHIVIRPKGYFCTGCEFKIWSEFSGKKITQAQAEKLVKSGKTDLIKGFKKKSGGT
                     YDTVLVLEDKKTGKLGFPVRAKK"
                     /name="type IA DNA topoisomerase"
     gene            complement(25486..26058)
                     /locus_tag="WAZ52_RS00175"
                     /old_locus_tag="WAZ52_00175"
     CDS             complement(25486..26058)
                     /locus_tag="WAZ52_RS00175"
                     /old_locus_tag="WAZ52_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001199073.1"
                     /translation="MQYALVDALERKFLLDALEFGVLKDWKENPVKELPDIDESVHPFH
                     ICYGGYLLNPGVSDSDISRKIKDQAGFWLAAIDDTRMDCHSIAYYDIHTLPLISCGHQK
                     IVPFAALIKADECIISKIASYSGFAVTAFLRIKEWDIATNILNREGIFAFNGCERRFRV
                     VSKDNWQHTVSEERAIRCAKRLIQCKG"
                     /name="hypothetical protein"
     gene            complement(26062..27324)
                     /locus_tag="WAZ52_RS00180"
                     /old_locus_tag="WAZ52_00180"
     CDS             complement(26062..27324)
                     /locus_tag="WAZ52_RS00180"
                     /old_locus_tag="WAZ52_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000517489.1"
                     /translation="MGKLTLVEQALRLHKEYYGEDNFGTVVKDCDYYPKVAEIFINRKN
                     RKIYSNLDIEPVASTVVHTGSNPVMAKAGRIKKLMFKDGSGAYRIHLGQNEVVHIIRFI
                     LNSKVRMEYAVGTTKSMSMLHNLCEAGRAKLNRSLPRKGIWKLGYYDGVYYHGKARKEE
                     IENAMRFSVHPKFDELENDFNQFFSDIDFYTRYGQSGMRKVLFTGPPGTGKTTIAKALG
                     AKYQDKYVFVYADDYFKDVCYAAAQKKIPVIIIAEEVDELYRADAGTLSFLDGADTPRN
                     VAGTYVIFSTNYPKRIDPRIRKRPGRIDRIISVGAFRTKAAAACAKMYLPEDVEIDLKE
                     LGAVLDRTTPAEIKEIINIAIGMIRGTKNELSVDVIKNARAFLKGTLDLSVQEADEDIE
                     EREELYKKNGAQPDYLSYLED"
                     /name="AAA family ATPase"
     gene            complement(27406..27900)
                     /locus_tag="WAZ52_RS00185"
                     /old_locus_tag="WAZ52_00185"
     CDS             complement(27406..27900)
                     /locus_tag="WAZ52_RS00185"
                     /old_locus_tag="WAZ52_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="WP_001215681.1"
                     /translation="MRHFLKALSFVVSLLPATLCAAEIQGKVIRVLDGDTIEVKTLPAK
                     IVVYEVPIRVRLINIDAPEKKQAFGHWSANQLKALLAGQSVTVSYTQTDRYGRIIGRVF
                     TTNGTDASRFMVQSGAAWVYERYNTDKSLPALQREAQEQKRGLWADSNPVPPWEWRHKQ
                     N"
                     /name="thermonuclease family protein"
     gene            complement(27897..28205)
                     /locus_tag="WAZ52_RS00190"
                     /old_locus_tag="WAZ52_00190"
     CDS             complement(27897..28205)
                     /locus_tag="WAZ52_RS00190"
                     /old_locus_tag="WAZ52_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000647577.1"
                     /translation="MKARSVRVAGIEIIDSVKTVAYVFTEALLFMVWFSAIVYGLYRVV
                     IVLWDDPLKPQSLMEFLNFIVELYSYVLPSLAVIAVALIAVLSICKILSAIWEGRKQ"
                     /name="hypothetical protein"
     gene            complement(28297..28545)
                     /locus_tag="WAZ52_RS00195"
                     /old_locus_tag="WAZ52_00195"
     CDS             complement(28297..28545)
                     /locus_tag="WAZ52_RS00195"
                     /old_locus_tag="WAZ52_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001290044.1"
                     /translation="MSEIRFVTLESDFNNIKEIYSDDELNRLLEAVNQNCPTRIVLGSV
                     EKGKETPLKWCFRTIPERISIEEIDEIKFIQNPSEQG"
                     /name="hypothetical protein"
     gene            complement(28600..28905)
                     /locus_tag="WAZ52_RS00200"
                     /old_locus_tag="WAZ52_00200"
     CDS             complement(28600..28905)
                     /locus_tag="WAZ52_RS00200"
                     /old_locus_tag="WAZ52_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_000147045.1"
                     /translation="MTGKNRVVLPALLALGLISTNANASDPCASVLCLYGKAVGQGGGS
                     ECRSAEKDFFNILKKKKGSIRWSKTFDARKAFLNQCSTADPAAISKIMSKFGRVKG"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            complement(28889..29311)
                     /locus_tag="WAZ52_RS00205"
                     /old_locus_tag="WAZ52_00205"
     CDS             complement(28889..29311)
                     /locus_tag="WAZ52_RS00205"
                     /old_locus_tag="WAZ52_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="WP_001328552.1"
                     /translation="MMKKLVFLLISILAGCSSPPEPTPVQFEKANEVINPSLPYVPDFH
                     GVIKSDVSGKGWVYEITSLSGVQARTPTFYYALAHADRIVVTTHDAGLWFRIRDLLKME
                     GATAVVEWRNEKSFLPEQGKIVFIKAQNEVKNDWKK"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            complement(29320..31182)
                     /gene="taxB"
                     /locus_tag="WAZ52_RS00210"
                     /old_locus_tag="WAZ52_00210"
     CDS             complement(29320..31182)
                     /gene="taxB"
                     /locus_tag="WAZ52_RS00210"
                     /old_locus_tag="WAZ52_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxB"
                     /protein_id="WP_000847103.1"
                     /translation="MKTFNKSQSVFIFAVMLVAAWFAGSFIFFVLYFSQIKNLKPLKAV
                     FRSIDAYRMDIFTDSLTLSVVTTDIRVLAFVALGAGFVVSLIIPVAALIKLNEKKDNIF
                     GDARFATTNDIRESNSFTLDGDEKDGIIVGIKDKKIIRYVGAAFSAMGAGTRAGKGAGI
                     VITNLMKYWWSVIILDPKRECFNITSLIRKVILGHEVYKFDPFSPVTHRFNPLYYVSMG
                     TSEGFNQLENLALIIYPYKTDGADAGSYLNKTAGGVFKSYAVALWFMIKNDKAGLKTLD
                     IEPVFSMSKINQLFERAEPEHLLSFMNDIRSELRGKDKTLADIGVAGLKAFIEMEDKTK
                     AQLKSNFLNGLSPFANPNVANATDGNDFDLRQVRKKRMTIYFCISGDNARLAEKITNIF
                     FQLAIQVNLEKMPTDDPEIKHDCLFLLDEFPSIGAVDYIKSKSGLIAGYKLKLLIVYQV
                     GSQLEEIYSYAGSKTLLASAPCKIVYSASDVKDARELSEAMGTRTVTIGSKSKSRSRGG
                     TSRSESESLIERPLVTTNELLTLKFSEEILAMKGENPIRCEKAFYYLHEYFFGDFVKVA
                     PELANIFPRKKGKLVMPPQKVFENEIVAKGYLAVKEVPDLDKAA"
                     /name="taxB"
     gene            complement(31169..32197)
                     /gene="virB11"
                     /locus_tag="WAZ52_RS00215"
                     /old_locus_tag="WAZ52_00215"
     CDS             complement(31169..32197)
                     /gene="virB11"
                     /locus_tag="WAZ52_RS00215"
                     /old_locus_tag="WAZ52_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401678.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_000017224.1"
                     /translation="MSGVIPVSISNKSLDFYKNQVFQSFLDIEGLTEIAVNRPGEIWTE
                     INGDWTFHNNDGVTFDFCKRFSQTLASFRGDEIGDTKPLLSATLESGERVQVVFPPACE
                     RNTISITIRKPSTRQITHKEYVANGFYDYVQSGKKHRTYDDELLDLFRARNIAAFMELA
                     VAAGKTIVFAGATGSGKTTYMKSLIDFIPLNTRLITIEDAEEIKFFIHKNYVHLFYPSE
                     SGSDSGSIITAAKLIKSCLRMKPDRILLAEIKGGDAWDFVKVAGSGHSGSMTSIHAASA
                     KDAIIQMVTKCYQNRECQNLPFDVLRKIIMDSIDIVVHVGRDGVVRHMSDIYYKGAECE
                     NF"
                     /name="virB11"
     gene            complement(32199..33317)
                     /gene="virB10"
                     /locus_tag="WAZ52_RS00220"
                     /old_locus_tag="WAZ52_00220"
     CDS             complement(32199..33317)
                     /gene="virB10"
                     /locus_tag="WAZ52_RS00220"
                     /old_locus_tag="WAZ52_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="WP_137532748.1"
                     /translation="MTDMKKTDDLVDDANQSKLESKPVHDIGDIRNKNNRMRSGFLFGL
                     MAVLVIGIFALKTYKNYFADDQQKASESTGDTSISQVSKIRTGLGQNFDPVENKAIINP
                     GATGSGNTVSDGHKEVLQEGFRKYLSIPVAGQGGSQTGASGSSRTSQTSEPQEERKESK
                     ENVPGKSGMKVTAINLDPDLYIEENRLIPCALTTRFVSDVAGRISCVFTEDVWSANHHT
                     KLLEQGTKAFGRYQTGTLNHGQGRMFVMWEQLRTPDNKRIDMVNTAAAGPLGEAGIDGW
                     IDSHFWERFGGSLMLSMVQDVAAAAADNAPGKDRNVDYTENSRQAMAEMAKVALENSIN
                     IPPTMYKNQGDIITIMVGEDIDFSDIYELKVK"
                     /name="virB10"
     Typing          16273..17279
                     /name="IncX4"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.802"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8092..9317)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4..392)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="5.51e-80"
     Typing          complement(393..1075)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.4399999999999998e-137"
     Typing          complement(1615..2684)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.72"
                     /coverage="100.0"
                     /evalue="6.640000000000001e-173"
     Typing          complement(29324..31182)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.839"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(33314..33825)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.54e-98"
ORIGIN
        1 attagatcaa gactgtaaat ccgctttgtt gttctgacaa aaagattcgt gtcccattcc
       61 ttttctgttg gctggaaaac ctttttcagt ttttcgcctt catcgccttc ctgttcctga
      121 acaacgggac gcggattgat gtaaacaaca ttatcatcgg ttgtgatttc ccatccggca
      181 ggaaaaccag cctttgcact gataacagcc tcaccttcat caaaaacaac tgatgtgaga
      241 aatcctgtct ttgaccggat aacggtcgta ttcgcaccgt tataactcac agtctgcatc
      301 cttgcatcaa atccagaagg taacggagtc gctgctgaat acacaagccc aggaagcaat
      361 aaacatgtag caataaataa tttcttcatc attcccccct gacttcccga tctgtctggt
      421 aactggttac cgtaaaaccg agaggattaa gctcacgact ggaactttta accgactttt
      481 ctggttcaaa acgatatgtc acacgagccg tccagtatga aactgaaacc tgtccggtcg
      541 tgaagtttct cgtcgttttt ttaaaacgca cagaggccag tttatctggc gcggttgcgt
      601 cagaaataat attggagatg acctcaaccg caacgtaaga accgttatta tgataaattt
      661 tatcaggagc catatcgctg ttatacagac gaagataatc gtcctttact gtattcgagc
      721 tatatagctg tacagtctca taatcatact gaagagacgg ataattataa ccttcacgta
      781 aagtaatata cgtgtttaca aaataccgcc caagcgcttc agattgcagg atcttttcct
      841 gtttaacctg agtaatcagt tctgtacggc ctgttttttc gccaacagcc acaatagcaa
      901 gctctttttc ttttaatggc acaagaacac aaattgcagc cccaagacat aaggacagta
      961 atgatgctgc cactgcaaca cgccatgcat ttttcttaga ccgttcattt tctgcataga
     1021 gttgtctttc gaaggattta gctgtctcaa tcttcttttc gattttttca ctcataactg
     1081 attacctggc tgtggttaat gggttccagt tcgccggaaa catcagaagg aagggtattc
     1141 cttgtggtac atccggaaat ctgaaacagg aaaaacaaaa gaatcacaca tcgcataaat
     1201 tcaccttatt aagcgttcac gaacaccttt aagagataaa ggccaagatg aagaaaaaga
     1261 atcccaccaa taagaagcaa aaagcgaaga tccataccct ttctccattt ccccgcaagc
     1321 cctaaaccaa gtcttattcc tgcgaagcag gtaaacactc ctaaaagaag gattaggaca
     1381 aaataaagta tcatgacaaa ataatgaagc atgtcacaac cagctctgac tgattccacc
     1441 taagacctta aataaggtaa ttcccgtgat taaaataata agctgggtta ttagctttct
     1501 cttcctgctc catccggagg tgatatgttt atcaacaaac gtcagtatcc agttcaggga
     1561 catcacataa aaaaaacgtt tcattatatt catgcggcat tcctcgctcg tgctttttcc
     1621 agggatgcaa gtcttctcgc cttcatcccc aaagcacctg tcggagccag cgcctgatat
     1681 cccttttcac ccgcccattc agcggctgtt cgcgcaccac gccccatcag attccctgct
     1741 ttcccagata aagaattcat gcccgttctt ccggccatcc ctaagccaaa acctaaacca
     1801 gcacgaccgc caagcatcag agacttacca gtagccatac cagcagcacc cagccccatc
     1861 atggctgcgc cctgaacagc accttcaaca cctgcacctg ctaactggac agcaaaacct
     1921 ttagctataa gaaccaataa agcccccaaa aacccagcca taaagcccat agcacctgta
     1981 gatacaagat ttacatttcc tgtttgtgca gcacgaatag catgggaaag gatcatccct
     2041 tgaaaatcca ttgctatccg aatcactaaa ctggcaaata gaactgtcag aatactggag
     2101 aataacgatt gtaaccaatt attaaacatg ctccgaataa aaccaaacat aagacaaaag
     2161 ataaatatcg gtgctgtaat aatcaggagt ttcatcgtta catctgctgt taaaaaaaca
     2221 accacactaa cagccattaa aataagcgag ccagtccata ttaaaaactg tccaactaca
     2281 ccttgatctt taacatatgt tgatttatct aaagagtata tttccgcgcc aagattttgc
     2341 gtacttttcc ataagttatc taaagtttgc cagacactca cccctccaga aaaaccatct
     2401 ttcatgcctt gcaacgcatc agtggccgca gttaaatacc catccgcatt agttataaac
     2461 atgattatga ttgcaaattt tgatagatcc catactaaat cctgcaaggg cgtctgagtt
     2521 ttactggcaa gtatttgata tcctttccat agtacatata acgtaaccga taccgtcgct
     2581 aacgacataa tatcattagc aaattgtgtt tgataactgg agctgatttt atctaccgaa
     2641 tccataacgg tagaattata tttaacgaaa aacccctgag ccatatatcc accttttaat
     2701 caaaagttaa tggttcagct tctttctgct gtttaataaa ggcctcttgt tcttgtgtga
     2761 tcaacatctc atgatctgct ttatttttat tattccagac atcatactgc atttttatcg
     2821 cctgcatttt taacgactgc gcctgtaatg cgttagccaa atcctgggat tctttgatat
     2881 cctttgaagc ctcaattcgg ctacttaaac tctgaatctg agacattgca tttgataact
     2941 gcttattgat ctcatttccc tgttcaatac tggcagccgt tgacacaatc cgtgctttac
     3001 agagattatc attacgttca tagcccgtat tacacatatc gaaggctcca tacttgctga
     3061 acagattttt agcctggctg ctcaatgcac cttcgggatt actggtaaaa tcagaaatat
     3121 aactgttccc ctgcttataa atatttgtca gttctgattg tagattactt agttcatttg
     3181 tgaatgccgc aatattcctt atacctgttg ccgtggctaa ctggtcttta tatgcctgaa
     3241 tctgactctg ataatgttgc gcagtttcgg accattgttt gagtcgttct gtccatcttg
     3301 cggcttcaac agcccattcc ggactggcat caatagcaac cggaattcct gcgctactat
     3361 aaaatgaagc cgtcaataat gatatcagca taaatgtttt tacacgcttc ataaattcac
     3421 ctgtcagatt gcccgttcga gatattcagg aacccagtca tcaggctcca ttccttcatg
     3481 ataaatactt tcaaaaattt ccagattatc tgcactagca gacaatattt tcagatatcc
     3541 accaagtccc gataaatcaa gtgtcgcaag tgctgaaaat gatttaccat cccccttttt
     3601 aagactgctt tttttaatca ggaactgtct cgacattgga tcaagatttt taacaatatt
     3661 aaattcttct ggtgtcagct taagcccttc cacataatca ttgtaatcgg catcagggtt
     3721 agccagataa atactggtgc tgcatacttc aaccacagcc cgtgaaatct tattcttcag
     3781 tatttcatcc ggagactgag ttgccggaat aaccagcccg ttcagcttac gaattgtttt
     3841 cagcttgtta tagacaaagt cgctgaatgc ttcatcctgt agccatttcc agaactcatc
     3901 caggaagata acaagacgcc gaccgtcaag aagctgcgta atgcgataca gcagatagaa
     3961 agtaattggt gcacatacca tcggatcatc aagaaactcg gtgccatcaa tgccaaaatt
     4021 attgacatgc tgaatattga atgtgtcttt agcattatca aaaacccagc catgagcctg
     4081 accattagcc cattgggaca accgaaggat aatcccgttc tcctgctcat cccgatcatc
     4141 tctctgcata agatgctcaa gcattctggt aatgccatat tcccgcattt cgccaggagg
     4201 aaaatccatc acagcatcaa cactttcgct aataagcaat ctttctcttg tcgataatct
     4261 ttcaccattt ctggtacaca gaatctccat cagttgcttg acaaaaatac ggttacgttt
     4321 tgttgcctcc agagcaaaag gattccatcc tgtcggctca ccgcttttta cggtgtaata
     4381 ttccccacca agcattcgga tacataattc agcccctctg tccttatcaa gaaagacaca
     4441 cgttaacttc ttattttttg cagaatcagc aaaagtttcg gggttattgt atttctgtaa
     4501 tgaacaggca agatatgaca gaaacattgt cttacctgaa cccgccgttc ctataacttt
     4561 agtattagca agattctttt cacctgtttc atctttaaac agtactgagt tatgtaaatt
     4621 cagataatac gcctgtttgc ttggtgtttt gagaatagca accgcttctg tccagcagtt
     4681 tttatcgcgt ttcccctggt agaaattatg gaatgatgcc agttcgacaa aatttacatt
     4741 cgataccgga gataaacgtg gccttaaatg aaaaaccgcc ggaagctggg caaaatatgc
     4801 cgctggcaat gacatggttg acaatgccgg aataatgccc aggtcagtaa agtctgccgt
     4861 aattttactt acagattcgt caagctccct gatactgtcc gccattacca ttagcgtgaa
     4921 atggtagtaa ccaaaggata tatcaccgga cacaatatca ttttttgcct tttccagttc
     4981 ctgcaattga gtaaccgcat catcttctgt actctttaac tgtttttccg cacgtttaat
     5041 ggtactcaat gcttctgatt tgctcatgga tgtatatgaa tgcgtaataa tatagtcagc
     5101 atcagaatac tggagcacat caaaaacacc ggatgcagtc tcactacaga aatctttaat
     5161 ttcaatagaa cgaaagaatt tatttccatc aatgcggcat atctgtccgg tatcatggtt
     5221 aaaaaacaca tccactccac caagaacgtt atacaccgga gaatcagtta ctctgatttt
     5281 ttgcaacttc cctgtcagaa gataattata gaatgacagt tgagaagaaa atacctttcc
     5341 gttctcctca tacataccaa gaagtttaca ggtaaattta tcaagagcgc cagagaatgt
     5401 attaatcatt tcattcattc tcttcacatg aatgttaata tcatctttct tttctttaat
     5461 actttttttg cgtttttcca gtttttctac acgcccgtct ggtctgtaga tcagcgtaaa
     5521 gtaaagagtg ttccccttga atttattttt cttcatcgaa ccataataac aatctgatat
     5581 tatatcagca tatttattac ctgattttgt ggtaaaggca tcataaaaac tcgccctgca
     5641 tgaatggtta taaaatgaaa cgggcagtcc ggaaaatgaa cgaacaagag tggcaacctg
     5701 tgagtcaata gtttctaaca tctccgcaga gattgactca aagttaatcc ccatcagttg
     5761 ccatgtcgct acataatcac cgttttttgt tttgacgatg tttttatcaa cgtggcttga
     5821 atatgggata tatttaccag tagtgattcg ctcattcagt ttcatggcat taacaaactc
     5881 atcaatttca acagcttcat attgtccgga gaggaacgct cgtgcgccaa aaaaacgatt
     5941 acacactgag tttccgagca ttttcagctt aagaaagtaa aggttaaaaa tgtggtcgtc
     6001 ctgtttagtt atcattctga gaacaaaaac cgcgacagga atagccagcc agatcagctt
     6061 gctgatataa acccccgcca gaaataacgc ccctgcaacc acagtaagag ggacgacagg
     6121 aacacccaca tacatagcag ggcgtgtcat cgctttataa agtgtagaca tactatcgtc
     6181 cccgctttat tagttaacca tcattgatgc aacttcagcc gcactggcga taacaattgc
     6241 cccgatgatg ataggtgagc attcacgaat cgtgcttcca ccaaacagaa ccttgtagcc
     6301 gacccacaga caggcaatag tcaccgtgac cagtgaaaga ccactaagcc ctgttttaac
     6361 cttttccagc agcgtttccg cttttgaaaa tccatccgca aaggccggtg atgcaactgt
     6421 tgccccggcg actgccataa ctaccttatt tttacgctgc attacagcag tgacagatga
     6481 actgatacga ttacatactg atttcttaaa tgacatttta taagtcctgt taattaataa
     6541 cttttgttgg atgtgagtta tcaacaaaag cagatttcag aatggtttcg ggccagataa
     6601 ctgacgcatt gtgtgaacga ttttttcgct gttctccccc gttgcttctt gtacctggta
     6661 caacatattt tttttcgttc cctgtataac caattcgctc aacataactt gtattattaa
     6721 attctttttc ttttcgctta ccgttactaa aatttcctgt atagtaacaa ctaagcgcat
     6781 tctccaggct cctccctcgt ttataacaat ccacgataat cttttcgaaa acagacaagt
     6841 tttcacacgg atcaaggagt ttttctgcgg acgtggaata aaaagaaaaa ttactgttgg
     6901 taatctgcat caatccgaca gaataacgtc tttttctgtt ttcaatttct ctgataactg
     6961 taagagcttc ctccttactt ttgggcatat gactgataac acttttgcca tagctaccgc
     7021 gttccctttc aggaataatt tcagccacag cataaggatt gaagccactc tcgacccgtg
     7081 caatatcatt tatcgtatcc ggatgaactg acgccgcaca cagcaacaat gctgggaata
     7141 aagacgccat agctaacgct ccatatatcc atcaatacgc atactctgca ttaataagta
     7201 aataatttgc ttcctcttac taagcaaaga tgttttcgtt ttctcttcga taaatgcatt
     7261 caaccttgga tcgagtttta ttgtgacata aacccctttt tccgatgact tatctggttt
     7321 ctccttcatt taatcaatcc actataagtg tgttttggat ttgttttaga tgcgaacatg
     7381 attcgcaaaa taacaatgct caacaatacg atcaaatacc attgacaaaa tggtcatgtt
     7441 atgattcgtg tgatcatgca aaaaaaagcg cccatatggg cgctttagta tgatttaaac
     7501 attagttaat cgaatgtagg gactggagca tccttctgag actgaataaa caatgcttgt
     7561 ttaacattct tacagttctg agaatcagag ccagacttct cacactctgc ctgcttttgt
     7621 ttagcttccg taagatgctg cccccaccaa tccacacttt ttacttcttc actacaacca
     7681 acaacgaaga acggcaaagc aaaaagcaga tattttttca taaattcccc ctctcactga
     7741 tcctggaaag acttgacacg agaagggcat tagtccgaat gagtaccagt gttctgatcc
     7801 ctgccaacga gacacctccc agttcagctc ttacagaatc aagatatttt ctgacagttg
     7861 aaggctttag tcctttgaaa tcagcaattt cattcagggt atagccataa gcgtacatca
     7921 gaataatgtc tttttgttgc tcactcaaat gttcttcatc actaaaaatt tgatgaagtg
     7981 cagaaatgca gatagcatca tgctcagtca tgataacctc cctcgtaggt tgttgtgatt
     8041 agcaggtgag caaagtagcc gctttgttca cctgcgccga aatcaaatta aaatcccagg
     8101 cctttccagt tcctctcttg ctgtattctt agttttttat gttcttctga atcctttgta
     8161 aacccgtgtt caagagcaaa cccgatattg tgaccttgct catgttttct ggatagctgc
     8221 tttttaacgt cgctctcttc cggctgcctg gtttcacgct catgaacctg acgttctctg
     8281 ttccgctcaa gggcaatatt ttcaatctgc cagttattgc gatgcgtttc cctgtagcct
     8341 gcaacagttc catcatcatt aaacatcggg ctacgtacaa gcgtaggagc cagtttttta
     8401 actttgataa gcgttcctgg ctggagcttt tcagactcga aatgattttt caggtcttta
     8461 ccccaaatcg taacctgctt tccacctttt aaagtttcat aagtgaggaa aggtgttcgt
     8521 ttttcccctg ccttattctg atatgaagtc tccccaaaat ccacaacctt gtaaacattc
     8581 cggagtcttt tcggattttc agcctgtaat cgctcaatct cccgtttacg ttcgggatct
     8641 tttttccatg tagcggtaac atcatatccc atacctttca gttttctatg aaaaccgttt
     8701 ctgaacttcc tggtttcaga cttagtaagt cttaatctcc ttcctgtttc ttcatcacga
     8761 agtttaatat taacatgtac atgaggatga tcttctttat catcatggta tacggcaacg
     8821 aaagcatggt ttggataagt ctctttaaga aattctatcg tagctttcag agcgtcttcc
     8881 ctgcttactc cagcttctgg tggtggtgaa aagaccatgt ttttcacatg atcgatgttt
     8941 tctccacgat atgttctgga gtaatcattt cctttagata aagttgaagt aaactccctg
     9001 ttaaagtctt ctcctttccc tgtttgctcg gaaccttttc catcataaca gtaagcctca
     9061 agttcaccat tacgggtaat gtaatcaata ccgtttttga tacctgcggc agttttccct
     9121 gaacctgtga ttttaaaagt gacttctttt gttggcgtcc aggtatatgc tttcttgtct
     9181 gtgccatgtc tgactttatg attaaaagcc gatttactgg catgtatatg tcttcccgat
     9241 aacttaggag atttcggatc acgcccagtc cctttagcgc gtttaatacg gtattcctgt
     9301 tctacataca cacccataaa tcacccaaat ctgttaacaa cactcttggt taacgtttca
     9361 agttttgaat caatttgctt acatagttca atcacttcaa caacgtcttt tctgaaatca
     9421 ggatcgttaa ccgtgcaaaa tctttcacct tttatgataa aatgaatgtt gcgaccaaca
     9481 cgatctacag cagttgataa ttttttaagt tccttaactt catcagggta aagacagact
     9541 tcattcttta acaaagactg acgcacacga atagcaattt ctctgctcat gctcatgccg
     9601 tgaagggctg cattacgttt aacaagttct atttcatgag ggtataaaca ggttttaaca
     9661 gcctttgatg attcattgct gacgggtata tttgatggtt gaacatcaac attaagtaca
     9721 ttatgcctgg caaatgtatt gatgctggta aacccacttg atatagcgtt atgctctgca
     9781 agttttaact catcatcact aagtctgaac gctatcatct tcataactca ccccaacaga
     9841 gcctgcggca ggcacaggca aaaacatcgt ttttggacaa tcatggataa aatgttaaca
     9901 ttttgaccat gattgtctat cctgcattga ttgtaccata tgtggcactg tgcctggcta
     9961 taaaatcgtc gctgcatatg cccagatttc gttgctgaaa ggttctttcg tcttcgacca
    10021 tctggcaaac gtatagctga tagatttgtg aaagaaaaaa caggcctggc agggctggga
    10081 attgttacgg ctgaagccgt aacgcttttt aagtcctcgc tgcgctgcgg ggcttccggc
    10141 gcaaagcgcc tcccatgccc ttacagggca taaaaaacaa gggtaattaa gcccctgcgc
    10201 gatgattgct gacttctttt tccagagcct tattaacaaa ggcattcaac gataagtctt
    10261 cttccatcgc catttctgct acccgacgat gcagttctgg atcaagcctg acgttaaaca
    10321 cgcctttaaa cggggtatca ggttcctttc catcctccac acaagactgt aaatacagat
    10381 ctacacatag catttttgtc aaaaaataca aaggggcaga aagccccttt ttttatttaa
    10441 aacatttcat acccttgcag gtagtttaac caccaccgga attgatccca ttcactaaat
    10501 ttcattgccg gaattctggt ttcccagcct ttgacaaggg aatcagcaag ctgtacgatc
    10561 tgccgcgttg acatttaaaa acctcattca gttttgattg tcgataacgt gatcaggttg
    10621 gtggaacaac ctgatcacac cttgaatagc cagtcattac cctccttctt ttctatagtt
    10681 actgtgtatc cgtaaagggt caaaatgcga taagcatcca acgcctcacg ctcactttta
    10741 aatgttctga cttcgtgtaa tccattacct ttatcaaggg atacacaaaa ctcataatct
    10801 tcttctactt ctatcttttt aattaattta tcaagttcgg ctaatgttcg ttttggctca
    10861 aaaagaaatg tgcattgtac aatggcattt aaatcttccc gatcaagctc taacagagca
    10921 tcattataag cagctttaaa acagcgatta ttacgcagat cagatgcaaa ctgcattgcg
    10981 gcttgtttta acgttttcat gaatttccct tgcattgatg ttaatagaca atttcagaat
    11041 caatattgaa atttccagaa aatgcagggt tcccccctgc atttataatg cttaacgaag
    11101 gcactcaata aaatctttaa aactcaagca ttcatcgcca tcttttaacg aatcaaaata
    11161 tttttcatat tcgtccctgt agaaaaggac taaatcaaca tcactctgct taagttcgcc
    11221 aaactgagga acaattccgg ttttatagtc acgaatttta ttgttatcag taaataggac
    11281 aacatactgt tgtcccgtat ccttatgcat aaggataata gtgcaaagcg ttactcgttc
    11341 gaatgtgaaa acatgattag tggttgaagt aaaaacagat gagcatttca ttttaaatct
    11401 cctgccctgc tgggctattg tgagaggggc cattcccctt tcgatgaaat aataatacga
    11461 ctttcaagtc gtatttgcaa gtgttctccc gtttttttac tcgataagtg attgttatca
    11521 cgtttttaat ccgcgtaagc ggatggaagg ggcgaacgaa aataccaggg cagcacaggc
    11581 catcccttca ccctgcaaag cccaactgac cagcaggcca tgattatgct gatttgcgcc
    11641 gcagaatgcc gcagttagcg ctcgcgccag cgagtttacg ccgtccaggg ttcccctgga
    11701 cggggcgctg tactgcgcgt gtgagcgtca gcgagctacg ggcgcaactc caggtactaa
    11761 tataaaacca tcacaggtca ttttattttg actccctttt gcagagcacg tattcaggat
    11821 gttctcctgc aagtaacaaa agaaactcaa actcaaccag tgacagactt tgactgtttt
    11881 gagtattaac ttcctctttt ttctgccagg ttcttagccc aacaccataa atatgactgc
    11941 attccttttg tgttaatcct gcttttaaac gtaatttttt tacattttca ggtgtattat
    12001 cgggtttcat ttaacataaa ctccttaatt aaattcttaa ctggcgtctc atcgtctttc
    12061 cttaatccgg cacacgtaaa atcaatatgt tgtaattcat tctcattcag tacggcatca
    12121 tacaatgtga ttttcgtagt tttgttcccg attttaacat ttgttctggc ttttatcctt
    12181 tttgtaagcc tgtaattgtc accttctttt tcaagaaaat aagtcacatc acctttattt
    12241 tttctcataa ctgtttcaaa taccctgtaa ttttaacaag ttgatctata ctcaagcccc
    12301 gaatctgtaa atcataactg tcgccctgtt cggtgacttt accgttcaat ccgttttcag
    12361 ctttaaagcc tgtcaatgcc ccataccagc tttcaagctg gacaagttgc gataacatac
    12421 ctttcaggat cataatttcg tccttttttt ccacctggtg tttctcatac tcaatcgcct
    12481 gtaacacctc ctgcgaagca tttaccagaa tttttcgctc gatatcggct gcaatatcaa
    12541 cgggattccg ttttctggat aacgtgcaaa catgatgcgg actacgccag gagcgactat
    12601 ccacactccc cacaacagaa aaccggtctt tttccatccg aatatgcaca gagtaatttt
    12661 taaactgcgg tgacgtcagt tttatacgag acgaacaatc atcaagtttg ttcacgcgcc
    12721 agccgttccc caggatacgg cagacaactt caaagacagg gtgatattgt tcaaaaaaag
    12781 agttcatctg tttccctttt tgctacgtgg ctcctgttcc cgtctttttg ggttcgatgc
    12841 cccaaaagac gggctggcta taatatcggg ggcttctcgc ccccgttctg gttagactct
    12901 ggtttccacg cgttgccacc ctgtcgccct gcgctgtccg gttgtaccgt attttgcgcg
    12961 gatttcttct atcgtgtgtt cttcacgatt ccagtaactt ttatgttcgt ctggacgata
    13021 aaaccattgt tttttctttg ccgcccattt acacccgatt tcttttaaag tttccttatg
    13081 tgtaatagtt tctccactaa tccagaccca gttacctatc acttcaaata ccagaccaga
    13141 taaaccagaa agaacgttta atactttttc caggtcatca ccgtaattat atcgagcatg
    13201 ttcatcagta ctctggaact gatttatttt atcaatattt gccatcaata catcaaaagc
    13261 tgcatttacc gctttcatca gttcagcccc taacgggtta cgatctggat gatattttaa
    13321 ggcggctttt ctgtatgctg ctttgatatc tttttcagtt aattcgccgg ataatccaaa
    13381 aacgtttaat gcttcctgga tattcatttt ttaaatctcc tgccctgctg ggctattgtg
    13441 agaggggcca ttcccctttc gatgaaataa taatacgacc tttaagtcgt atttgcaagt
    13501 attctcccgt tttttttact cgataagtga ttgttatcac gttttttaat ccgcgtaagc
    13561 ggatggaagg ggcgaacgaa aataccaggg cagcacagga gccacatcgc cccgaagcgc
    13621 ggcgcagccg ctgacgaacg taccaggatg cacagggcca tcccttcacc ctgcaaagcc
    13681 taactgacca gcaggccatg attatgctga tttgcgccgc agaatgccgc agttagcgcg
    13741 agcgcaagcg agtttacgcc gtcctggtgc gtcgcaacag gacggggcgc tgtactgcgc
    13801 gtgtgagcat cagcgaacta cgggcgcaac gcgccagaat ctgctctatg ttaatgctaa
    13861 tgtatgcata cattagcatt aacacacgca tccacatcgc ctttaaatgt gtattaatac
    13921 acaccattaa actattgcca gtacgcaatt gtacgatttg ccgattaaat accggtgaac
    13981 gtcgggcggg ataactgcgg ttttgatggc atggaaattc ccccgcttag ggggaactgg
    14041 agagattagt ggctggattt actgagagtg aactgtaatc cggctaccga tgtaagttgt
    14101 gcaagcgttg tgatggtcgg gttaccgttg ggggataatg cgcggtaaat actctgacgg
    14161 gcaaggccag atttcctggc agcttcggaa atcccccctc gcgcctcgat tacctgacgt
    14221 agcgcaacaa gaaaaccacc taaaccacca ggctcgttta tttcttccag ggcattagca
    14281 agataagcgt ttgcataatc aggatctgcg cgcaattctt caatcatggc ggcattgtga
    14341 tcaacggatg ccgaataatc atttttctgc gacttcacat ttttggttgc tgtcattttt
    14401 ttgacctctt tttaaaatct tgcagataac taacggcttt atctaaatcg gctgtctgag
    14461 tttttttatt ccctccaatg agcagaagaa ttatttcgcc atcttcgatt gaataataaa
    14521 ccctgtaacc aggaccgtaa tcaacccgta attcccatac accgtccctt tcgaatttat
    14581 gatctccaaa atttccgccc atagcgcggg caattctgga atccaccttt aacgctgcgg
    14641 tctggtcttt acgtcttaat tttttcatcc agtcggtgta cggaacctca ccgttttctg
    14701 tctgatatcg tttcgccgtg taaggcatct tgccgccctc attcccttaa cctgtgtaca
    14761 ttgtctctta taagtgacaa cagatcaaga aaaatgtctc tttaaagtga gaaatcctag
    14821 ctttctggaa ggttctgtaa cgcattctga gcattttttc cgtttcattg tacttagccc
    14881 tagtcttaac agcgtttaac gcctctcaac gctttttagc agccttgttg ctgtgatctg
    14941 tagtcatgat ccggatagta gttaaatctt gatcaaaatt tcttcaaggc aacgtgtgtt
    15001 ttgtgaatac cccctaaaac gcgctgtatc gcgttttaag cgattttttt catttcgcat
    15061 ggttttcctc atggttgtat cgaacgaagc ggcagagagc gattcagggc ttctctgtga
    15121 cactttgggg aggggcattg ctggatctgt tgatttatgt atcgttgaaa tgaagtggtc
    15181 acataaatca attggcaaaa gaatatatca tgttgatatt aaaggtattt tattgaaaca
    15241 tgagaagatc ctcggttagc aaaatgagga tcataattaa aagaactctt tcaataaaat
    15301 cagctagtta tattaaaccg ccctcggaat gaaatattca ggttgtgact aaacatgaga
    15361 actttctcgg tcttcacagg atcagattct gatgaacaga aagcctctgt aagcgttttt
    15421 cgcgcctccc atgccctaag gggtatttaa acccgtttcg tgcgcctgag acgcttctga
    15481 acgtgtttta taggctatca agcgactcat caccgaagga acaacaacag aattaccagc
    15541 gatatgtgag aaatgactcc gctcgccgca gtcgaacgac cgagcggagc gagttagtga
    15601 gcgaggaagc ggaatgtctt cgatgcagca tatgaatccg tacgctctgg tctgacctca
    15661 tcttgtgatt ttgctgattc caactcaaaa aattcacaat gcgctctatt ctttttttct
    15721 aatttctttt ttctaaaaga tagatcactc ctatgttaca tataatattt cctttttcat
    15781 ggtcgttttt tcttttgttt ttcaattgat tgcaatgcta actagggaca gatttatggt
    15841 taaaagggga cagatttatg gttatttgcg tttttatcag gggacagatt tatggttaaa
    15901 aggggacaga tttatggtca actagggaca gatttagtgt ggataacttt tttatccaca
    15961 ggttgtggat aacttttttt agattcaact cggattcgtt atggattctc tatgaatctg
    16021 aattggatta gtgacttaga tcacatttag gatgtttggc tcgatgggtt tttgatgatg
    16081 cattccctcc tggtttgctg tattgactgc ctctgactga gggtgaaaat gcgccgttca
    16141 gccaggtttg acccttcaaa caggggacag atttatggtt tattagctat atagggacaa
    16201 aaaaaaattg cgtccctaat ttgggtgtga gaaacttctt ttgattttta actcacagga
    16261 aggggagctg tatggtctta aagaataata aaaatagcga ctgtaatgat gttcaaagtt
    16321 tgttggcaca gggaaatcaa ttgctggaag gtgcatacga tataacgcta attgaaatgc
    16381 gtcttctgta tctggcattg actaagattg atagccgtaa accacagcct gcaaatgaat
    16441 atacattgtt tgcgaaggaa tacagggaca ctttttcttt agattcgaaa aattgttatg
    16501 aacagttaaa atctgctgcg agttctttag gctcaaagcc aattgttacg tatgaatgga
    16561 acgaggcaaa aaaaaggatc gatgcagtca aacgattctg gttttcttca ataagatatg
    16621 gtgttgggaa tagtgaaagt gacataactt tgcgtttttc agattcggtt agtcaatatc
    16681 tttatgaact gaaaagcgaa tttacacaaa tgaatcttga acatatggtg aaactggata
    16741 cgccattttc atttcgactt tattcatggt tgtataaata taagaatctg aacagaaata
    16801 agaaacatag tggagtgatc tcgacagatc ctataaccat tgaatggatg aaggagcgaa
    16861 ccgggcttac agggaaatat ccagtatata aggattttaa gaaacgggtg cttgatcctg
    16921 ctgttgatat tatcaatgca aatacgaatc tgagtgttac ctatgaaggt attaagtccg
    16981 gcagaaagat cgagtctgtt gtgttcacat atcttgtaga gaatgaaaca catgatggaa
    17041 aaaaaatagc aactaaaccg ttacgccctc gattgccatc aagaccaagg gtcattaaag
    17101 gaagctctgc ggaggtagcc tgggcaaaaa aatgtatcga tgttatgaaa aattatctta
    17161 tctctttaag ggaatatgat gataagttaa aattatcaag gccagatctt cagaaggtta
    17221 agttatggca tgaaataacc ggatgggatt tcgaagagtc gttctggttt agtgattaaa
    17281 gaatgctact gtttacgtta cctgtttttt ctctttttat aactcactta aaagagagga
    17341 tgatatgaaa gataacatcg tggattttca acgtatcagg agagacctgg gcgctcattc
    17401 aacagatgtt gcaaatgatg ccgtttattc tgacaaaaag aaaacatcca ggttctttta
    17461 tctgctgaca cctgtcagat accttctggc tgcttccctt tatctggttg ttgttattcc
    17521 attggggatt cttgcgggat ttcgtttctt gttgcagttt ttaggcggta tcgcagtgat
    17581 ggggttaact atcgcctggt acatgggtgc agatgtcagt ggttacattg ttcttgctgc
    17641 atggatcgcc ttaactgttt gtggttgcgc agagcagatt tttgaatggt ggatcgaaag
    17701 ccgttttgcg ttccgtcttt ttggtatcgg gagaacgccg gaagaatgag cgctgatctt
    17761 acagacagta aagaacaatg caggatagac aatcatggtc aaaatgttaa cattttatcc
    17821 atgattgtcc aaaaacgatg ttttttcctg tgcctgccgc aggctctttt aaaaggaata
    17881 tgaaatgata tttaaccgta ataaaaaacg agatgaacaa cagaattctt ctgttgtttt
    17941 gccatcaact gaaaatgtat tctctgattt tattaagaac aatccagctt tgacaaccaa
    18001 tgcttttaca gccattgggg aggttgattg ttttactaaa gtacccttac tctcaaataa
    18061 ggaatcagag tttttaggca ttctttcacg taatattata cctgattatt ccgtttttcc
    18121 caaaatacga ctcattgaat ttgttcgtcc tcatggtagc gaggaagctg taaaagaatt
    18181 aatccgtgaa gtgcagaata taacctgtga ttttgtcctg aaatcatatg atgaaacagt
    18241 ccttgctgtt atccagtttg gtgaaactgc aaccgtcaga cagcaaaaga aaaaactgat
    18301 tatccagcgt gtgtgtgcta tgacagaaat ttcttttttc gaatttaaaa atactgtcga
    18361 tatgatgggg agcgaagact tttgtcagtt cctgcgagat gatgaagagt aaaaatatta
    18421 gaaattaaaa ataagcccac atatttgcgg gcttaacaaa cgcattgtca taatttattg
    18481 tttgttatcc tctaaaataa ttaataatgg tcctaacata gttgtaaata ccaatgctgc
    18541 tataccaatc ataccgtact tgtttggtac acatgataat aataaagagc acgtagatac
    18601 agttatataa aatattctga agttactttt tttaaatagt gatgatatat atattataca
    18661 taacgctata agattaaacg cgattgaatt tatcattgtt taatcctctt ttaattggtc
    18721 agcacatatc gatcttaccg cccagtaaca taaaaatgaa aaccgttgtt actgggagca
    18781 acattgccag gatgaaaacg attagattac ttttcctgcc aatattgaat actataccag
    18841 ccagcaatac taaaaagaaa aatgcaactg gtactgtcca tgtaatgaca ggatgatagt
    18901 ttataatgcc taaatcaaag atgcagtgtt ccatagattg agtgccctaa tgtctgatta
    18961 taaactggta ttattacgta tcctcacttc acaaaatctt ctggttttat acttgattta
    19021 aactcttctt ctttagaacg ttctttttgc catgctatat accatacaaa caaaacaaca
    19081 gttccaatga caaagtacga tgctggttcc agtaatgctg agatactgtc acctgaaaat
    19141 ttccatgcac cataacccgc aagaaccagt ccaataaaag gctgcacaca gagcatgtaa
    19201 aaattgaata cagatttaat tatctttatg agcatatttt tatccccttt aatagtgtgt
    19261 aataaaacac tctatttatt cacggttggt cttcctgctt tcgggctgtt tttccttata
    19321 tcatcaaaca gcctccgacg ttcttctgct gataccagac gtaacgctgc cagtgctgct
    19381 ctgattactt cagcacggct tgcaccgtac ttgttctgga acacctgcgc tgtatccaga
    19441 tcatcatctg tcatagtcag atttatacgc gttactgcgg atgccgatgc tggtttttgt
    19501 gcagcagaac ttacaaactg gcgtaaatcc agtggtttaa tttcctgatc tattttcttt
    19561 gggatctttg ccataaggta tcctttttat aaggtgtgta actatacaca tcatttatgg
    19621 cagggaaagt tccggcatcc acgtactgca aattgccagg cttggtcaga taagccatcc
    19681 ccgccgtata tcgcatcgga tatgggtata ttatcaaatt tggtgtgtaa taatacacat
    19741 tacagaatat ccgtttcttt taacaataac tcaatctcgc cacgggcttt gtttccccgt
    19801 ttatcatcgt attcatgtac gcccataccg ttgttatagg cttttttatg cgcagtacgc
    19861 atatagatac gctggcgggg aaccggaagc cagtctggat catcattgag ttgtcggcgc
    19921 agttctgacg cttcactgtc tgacgggtta gttgagcact tattcaggac aatccatgct
    19981 ttcattgacg gattgactcc ggcctgtgct gttttaatgt cgaaaaccag ccgtcccagt
    20041 gtgttgatct caatgcctga tcctggctca atgaatgtga aaagaatgtg agcacaactc
    20101 agagctttaa cgaacgccgg agatttcatg cctggtgtat caacaaacac ataatcaaag
    20161 cgggcggcca gcttttgtgc catagccgga atatccgtgt agcattcgaa atacggtatt
    20221 tctgacagtc tggcgttggt tttacgatcc tcgatgaaat ccttgatact tccctgtgag
    20281 tctgtttcca ggaaacaggc tgttttccct ttctttatga cacatacaca taaggaacaa
    20341 agcacggtgc tttttcctat gccgcctttg tgtcctgcaa ctgtaatgat ttttcccatc
    20401 acaaaatcct cctggttaaa tagtgtgtat taatacacaa tcttttgtgg tgaaagtata
    20461 accgtacaat tgcgtgctgg caatagttta atggtgtgta ttattactca ttattaaggc
    20521 gctcagggta cccaagaagc tgttttgcaa gctgtggatc gcaggtaaag gacaggccac
    20581 ttcctgtaag gacgtccagc gaaaaaacgc gggtgtaaat ctcggtgctt cttgcataac
    20641 ggtgtcccag cagggattgc aggacgcgcg gaggaacgcc actcattgtc aggtgcatgg
    20701 cgtagctgtg gcggaacgtg tgaggagtga ccggaaccga aaatgttacg ccgtcacgct
    20761 ctgcccgtgc cactgcggtg tttatccagt tcagcggtgt ctgtctcgat gttatgtccc
    20821 acacaggctg atgtttgatc cgctttttcc aggtcgccag gtattcccgc atacgcaatg
    20881 cgtatccggc atccagaaca ggaaccagcc ttacagcagg ctctggtggt tttctgtatc
    20941 gcggatcagg atgtaatggt ggatgtctgg tggtcggacg tcctggtctt ctttctgctt
    21001 cttttagacg ttgcttgagt gttttcagga tcaccacggg ttgcgggtac tgccgcgtcg
    21061 gagtcagcat aaaatcggca ggtttcagcg ccagagcttc gttcagtcgt gctccggtat
    21121 tccagagcgt ctcaaagtac atacgtatgt tcagatcatc gatgtaggag agaagcagtg
    21181 agacttccgg tgctatcaga tattttggca ttcctgcgcc aatctgtgat gcaagatccc
    21241 gcatattacg ggctgcatcg ggagagatgg ttaaattcca ggcgtcgcta cgtgaaatct
    21301 ggaaaatgct ggcgggtaag tcactcaatt ttttaatctc cgcacctgtt tttctggacg
    21361 tgccagcaat tatgacctga agcagaggtg cgtccgacaa caaaaattcc gatcaccaaa
    21421 aaaaaaattt ggacattttt tccgataatg catttttgtc caggtggata tgaacgacag
    21481 ggcagggcga aaaggaagcg gttatcctga ataacccagg atatgggcac atatgccgga
    21541 acagggcacc ggataaagga acagaatatt tgaatgttgt aactaatgaa gtctggacag
    21601 aatgacctgt atgtctaact gcttggcgaa ttaaactgga gcgagggtaa tcttgttcaa
    21661 aaatactgta gcggtcgcag ctgaggatcg cgctggccag cgtgctcgat gcgtcaccgg
    21721 cgagaagctg atttgttgtt ttacagtgag ttaggttgtt tgcagtgcga aaaatatcgg
    21781 tgagtatttt tgatagagtc attaacggcg gtgcgctacg tgaaatccgt gaaaaagcag
    21841 gggatatacg tggggtattt ttcacgtagc aacaccatta cagcctaccg ttcagcagac
    21901 catccatcat ttttcaaaac cagtgtaata tgttttgttt gtttctcttt caaatcagac
    21961 tcaagtccta aaaatgcagc ccgaacatca tcgcgtttcg cccattcagc gatactggtg
    22021 ggactgaatg tgtaattcac ctgcgtgatc ttcattccca tagcatcacc aggttcggta
    22081 aaatcaacta tctcatccac cttataatga ccgacgcaaa aatcaggtcg ttccggagac
    22141 ttcagatatt ttttcccctc atctgtcaga gcatattcac gacctggcac ttttttaccc
    22201 gtcttagtaa aaccaataac gtcatcaacc agggtatttt ttactgtcaa taaatcggct
    22261 ttgaccagta catccagcgc ggaaaactgt cgagaattta tttcatctgc ccgttcctga
    22321 gatgtgtatc gattagcctg agccacagct atctgaaccg gataaggctt actatcaaac
    22381 attcccagcg gactaatggt aatgcattcc ttctctagtc gagtattaat agctttttca
    22441 aagttttgct tattagcatc ttttggtgaa tcacaccctg ccagcaagaa tgaactcaaa
    22501 gcaacaaaaa taaccactac tttttttgat ttgatcatca ttttccctct ttatcaaacg
    22561 ataaaatttc ttgccagcgc cgctggccgt tctgtcccct ccatttgccg tcgcaaactt
    22621 gtttgcgacg gcatcacgat tattttattc aacctgccca ggttgcagtt tgtcaatgag
    22681 gaaatcttcc atgaaatggc cctcatcaag cagcttctgt aaagcaagcg gacgcttgcc
    22741 attaccagac cattctctga tctcgccatt ttcatcctta aagcggtatt tagcctttct
    22801 tactccgcct ttggctttcc gcctagtttt cactgtatca gctgttacag gagcaaggag
    22861 tgattcggga tcaagaccta aagatatgat gtattcaatg gcctgctgac gtttggcttc
    22921 aagttcctgg cgttcagccg cttcacgttc agcatcttca cgacgttctt caataacaac
    22981 gttgagcttt tcctgcattt cgagcaactg ctcaaaatca atctcacggg caaaaacacg
    23041 caatgaacga atattcatca tagtccggct gataatgccg tattcatctt ctttagtcag
    23101 ttcgctcata attaatcctc ttgtttagtg gacgtacttc cagacgccgg aaggaacctt
    23161 atcatacagt ttattcatca ccagttctgc gcggcgatga tctgcggcag aataaaatgc
    23221 ctctgattct gataaaggca gtttgtaacg gttaatctca ataacttttt caagagtatc
    23281 tcttgatgta cattttctta actgaaaaag ccattcctgc tttgttttca cttcttagcc
    23341 cttaccggaa aacccagctt ccctgttttc ttatcctcaa gaacaagaac tgtatcatac
    23401 gttccaccgc ttttcttttt aaatccctta atcaaatcgg ttttccctga tttaaccagt
    23461 ttttctgcct gcgcctgggt gattttctta ccagaaaact cactccagat tttaaattca
    23521 catccggtac agaaataacc ttttggtctg ataacaatat gtttaccaca ggaagggcag
    23581 ggagagtcca ggcgttgaaa ctgccctgct ggcgcagcgg gttcaatctt catctcgccc
    23641 aggtcaacat cagaaatcat gcctgtcagt tcaccgtaca gctcattaat aaactcttca
    23701 accgtcaggt cgccattttc aatggctgtc tgcttttcag accataatgc tgtcatatca
    23761 ggattaaccg ctataccagg cagggcatca ataagcgcat atccggtatc agtaggaata
    23821 agtttccctt tttccagcgt gatatagttt cttttcttca gtgtttccag aatggctgcg
    23881 cgggtagctg gcgtaccaat gccaccatgt tcatcttttt tgtctttatc cttatctttc
    23941 agcaatttct taatcgttgg gtcagtgaca aaatccgcga cgcgaacaag cgcagcaagc
    24001 aaggaggctt cggtaaataa tggcggcggt gttgttttct tctcattaac gacaacttct
    24061 tttgtcgtca gtgtttctcc tgtgcgaatt ttacagagca gttcaaaagc ggaatcatca
    24121 ttatcttctg attcaccttc gtctgtggtt tccgcgccaa gaaacgcctc aaatccgctg
    24181 tcagttgttt ttctggcacg ggcatagaac gactcatcac cacactgaat ggcaaccgat
    24241 acttcctggt atgctttttc aggcatgaac tgaacaagat agtgttgtgc gatcgccagg
    24301 taaacattgc gctcatcggt gctgagtgcg ttaacatcag gcacactggc ggtcgggatt
    24361 attgcagtat gcgcagtcac ctttgcactg ttaaacgctt tgcttttacg tgcagaatca
    24421 atatccagcg actgaggaaa gactgatttc agggcatcga taacctgcgg cgcttcgctg
    24481 aattgttcat cagaaagata tgagcaatca gagcggttat aagtaatcgc tttatatttt
    24541 tcacgtaatt gttgcgtaat atccagtgtt ttttgtgccg tcattttgaa ctttttgttc
    24601 atgtattgct ggagtctgac caggttaaac ggcaacggtg cagccgtttt tttatcatca
    24661 gttgctgccg cttcaactgt agccggtttt cctgcaaggg atgctgccgt tccgtctgcc
    24721 cacgctttat caagtaattt acggtcggtc agcggagcaa attcccctgg cttccagttc
    24781 gccctgataa catcagcacc acgctgaaag actccggtca tggtgtagta aaaactggat
    24841 ttatggttct ggttagcacg ggtacgattc acaatcaggc caagaacggg agtctggacg
    24901 cgcccgacag acagaacacc ctgatatcct ctggcttttg ccggaatggt gtaagcacgc
    24961 gtcatggaga agccatagac ggcatcagca actgaacgcg ccagcgcctt aaggtaaagc
    25021 cctttgaaat cacgattatc tttaagattt gccagtgcct ttttcactgc cggaagcgtg
    25081 ttgtcgttaa tcagaacgcg ctttacgggt tttgtgtttc ctgcatattc caggacttca
    25141 tcaacaagta gctgtccctc atcatcagga tcgccagcgt gaacaatttc agtcacgtct
    25201 ccacgtctga taagctcgag aatcgtttta acctgttttg ctgcactttc aacaggctga
    25261 tacttcacgg gataaagacg taaaggaagc gtttcaacct tccaggcttt gtattcagga
    25321 ttatagtttt ccggcggctg tgattcgata atatgaccaa aacagttagt cacaatggcg
    25381 ttatcacttt caaaccagcc atcatggcgg gtaaaattgc caccaagtgc cttaacaata
    25441 tcatttgcta ctgcgggttt ttctgcgata aaaagtctca ttttgttatc ctttacattg
    25501 aattaatctt ttggcacaac ggatagcgcg ttcttccgat actgtatgtt gccagttatc
    25561 tttacttact actctgaatc tgcgctcaca gccattaaag gcaaaaattc cctcacggtt
    25621 aagtatatta gttgcaatat cccactcttt aattctcaaa aaggctgtta cagcaaaacc
    25681 agaatacgaa gcaatttttg aaatgatgca ttcatcagct tttattaacg cggcaaaagg
    25741 aactatcttt tgatgaccac acgaaatcag agggagtgtg tgaatatcat aataagctat
    25801 tgaatgacaa tccatgcggg tatcatcaat agctgccagc cagaatcctg cctggtcttt
    25861 tatttttctg ctaatatctg aatctgaaac accagggttt aataaatatc caccataaca
    25921 gatatggaag gggtgaacag attcatcaat atcaggaagt tcttttaccg gattttcttt
    25981 ccagtccttc agaacaccaa attcaagagc atccagcaaa aattttcgtt ccagcgcatc
    26041 aactaatgca tattgcatgt gttaatcctc caggtaactt aaatagtcag gttgcgcccc
    26101 gttcttttta taaagctctt ccctttcctc tatatcttca tctgcttcct gcacgctcag
    26161 atcaagggtt cctttcaaaa aggcacgagc attttttatt acatccactg ataattcatt
    26221 tttggttccg cgaatcattc cgattgcgat attaataatt tccttgattt cagccggagt
    26281 cgttctgtcc agcactgcgc caagctcttt aaggtcaatt tctacatctt ctggtaagta
    26341 cattttggcg caagcggcag cagctttggt tctgaaagca cctacagaga ttatcctgtc
    26401 aattcgacct ggacgtttcc taatacgtgg gtcgatgcgt tttggatagt ttgtagaaaa
    26461 gatcacatat gtacctgcga catttcgcgg tgtatcagct ccatcaagaa aacttaacgt
    26521 tccggcatca gcccgataaa gttcatcaac ctcttcagct ataataatga cgggtatttt
    26581 cttctgagca gcagcgtagc aaacatcttt aaagtaatca tcggcataaa caaaaacata
    26641 cttatcctga tactttgccc ccaaagcttt tgcgatagtt gtcttgcctg ttccaggggg
    26701 gccagtaaag agcactttac gcatacctga ctgaccatac cgggtataga aatcaatatc
    26761 agaaaagaac tgattaaagt cgttttccag ctcgtcaaat tttggatgaa ctgagaaacg
    26821 catagcattt tcaatttcct cctttcttgc cttaccgtga taataaacac catcatagta
    26881 cccaagtttc cagatacctt tacgtggcaa tgagcggttt aatttagcgc gtcctgcctc
    26941 gcaaagattg tggagcattg acatactttt agtagtccca acggcgtact ccatcctgac
    27001 tttagagtta agaatgaagc gaataatatg aacaacttca ttttgaccaa gatgaatcct
    27061 gtatgcacca gaaccatctt tgaacataag ttttttaatt ctcccagctt tagccataac
    27121 agggttactt cccgtatgaa caacggttga agcaacaggt tcgatatcca gattcgaata
    27181 aattttacgg ttctttctgt ttataaaaat ctctgccact ttaggatagt aatcacaatc
    27241 ctttacgaca gttccaaaat tatcttcacc gtaatattct ttgtgaagcc gtaaagcctg
    27301 ctctaccaga gttaatttac ccataagcgc accatgtata tttaaaaccg tgacactgcc
    27361 tgaaatttat ttaaggtagt ggaggggttg gacaagcaac gcgcgtcagt tctgtttatg
    27421 tcgccattcc cacggtggaa ccggattact gtcagcccat aaaccacgct tttgttcctg
    27481 cgcttcccgt tgcaaagccg gtaatgattt atctgtgttg taacgttcat atacccatgc
    27541 agcgccagac tgaaccataa agcggctggc atccgtgccg ttcgtcgtga acacacgacc
    27601 gataatgcgt ccgtagcggt ctgtctgcgt ataagagacg gtaactgatt gtcctgcgag
    27661 cagagcttta agctgatttg ctgaccagtg cccgaaagcc tgtttctttt caggagcatc
    27721 gatatttatc aatcgaaccc tgatcggaac ttcatacaca acaattttcg cgggtagtgt
    27781 tttaacttct atggtgtcgc catcgagaac gcgaataact ttcccctgga tttcagccgc
    27841 acacaatgta gctggtaaca gggaaacaac aaaggaaaga gccttaagaa aatgtctcat
    27901 tgctttcttc cctcccatat tgctgagagt attttacaaa tggataaaac ggcgattaaa
    27961 gccactgcga taacagccaa cgaaggaagt acatagctat acaactcaac tatgaaatta
    28021 agaaattcca tgagcgattg cggctttaaa ggatcatccc ataaaacgat aacaacacga
    28081 taaagcccat agacaattgc cgaaaaccag accataaaca gtaaggcctc agtaaaaaca
    28141 taagcgaccg ttttaacact atcgataatt tcaattccgg caactctcac acttcttgct
    28201 ttcatatata cctcatggat aacgggaaac taacgccctt cggttgttca accccgcaaa
    28261 tcccagatta aaatctggaa tctgcggcgt ttacctttat ccctgttcag aaggattctg
    28321 gataaatttt atttcatcga tttcctcaat tgaaatacgc tctggtattg ttctaaaaca
    28381 ccacttgagt ggtgtttctt tccccttctc cacgctaccc aacacaattc tggtcgggca
    28441 attctgatta acagcttcca gcaaacggtt taactcatca tcagaataaa tttctttaat
    28501 attgttaaag tcggattcca gagttacaaa tcggatttcg ctcataattt ttctcctgta
    28561 tgtccactga tacccgttgg cgcgggtatc aggttttaat cagcccttaa cgcgtccgaa
    28621 cttactcata atttttgaga tagcagccgg atcagccgtt gaacactgat tcagaaatgc
    28681 tttacgggca tcaaatgttt tactccagcg aatgcttcct tttttctttt tcagaatatt
    28741 aaaaaaatcc ttttcagcac ttctgcactc actccctcca ccttgcccga ctgcttttcc
    28801 atataagcaa agtaccgaag cgcaaggatc agatgcatta gcatttgttg atattaatcc
    28861 taacgccagc aaggcaggta aaaccactct attttttcca gtcatttttc acctcgtttt
    28921 gtgctttaat gaaaactatt tttccctgtt caggtaaaaa acttttctcg tttcgccact
    28981 ccacgactgc tgttgcccct tccatcttca acagatctct gattcgaaac cacagcccag
    29041 catcatgtgt tgtcactaca atcctgtccg catgagccag agcgtaataa aacgtcggcg
    29101 ttctggcctg tactccggat aaggaagtga tctcataaac ccatccttta ccgctaacgt
    29161 ctgactttat gacaccgtga aaatcgggga catatggcaa tgagggattg atcacttcat
    29221 ttgccttttc aaactgcacg ggtgttggct ctggcggtga tgaacaacca gcaaggatag
    29281 aaatcaataa aaacactaac ttcttcatca tgttctcctt tatgccgctt tatcgagatc
    29341 aggaacttct tttactgcca gatacccttt tgcgacgatt tcattttcaa atactttctg
    29401 tggaggcata accaactttc ctttcttcct ggggaaaatg tttgcaagtt ccggagcaac
    29461 cttcacaaaa tcaccaaaga aatactcatg gagatagtag aatgcctttt cacaacgaat
    29521 cgggttttct cccttcattg ccaggatttc ttcactaaat ttcagtgtaa gtagttcatt
    29581 tgttgttacc agtggacgct caataaggct ttctgattca gatctcgatg ttcctccacg
    29641 actacgactt ttgcttttcg aaccgatagt tactgttcgt gttcccatcg cttctgataa
    29701 ctcccgcgca tctttaacat cagaagcgga ataaacgatt ttacagggtg cacttgccag
    29761 taatgtttta ctgcctgcat aactgtagat ttcctcaagc tgagagccga cctggtaaac
    29821 aatcaacaat ttcagcttgt aaccagcaat gagtccagat ttactcttga tgtaatctac
    29881 agcaccaatt gacgggaatt catccagtag aaaaaggcag tcatgtttta tttccggatc
    29941 atccgttggc atcttttcaa gattcacctg tatagcaagc tgaaagaaaa tatttgttat
    30001 tttttcagcc aacctggcat tatcaccaga aatacagaaa taaattgtca tccgcttttt
    30061 ccgcacctgc cgcaaatcaa aatcatttcc atcagtagca tttgccacat tggggttagc
    30121 aaacggtgat aatccattaa gaaagttgct tttgagttgt gcttttgttt tatcctccat
    30181 ctcaatgaat gccttaaggc cagcgacacc aatatcagca agcgttttgt ctttccccct
    30241 taattctgaa cgaatatcat tcatgaatga aagaagatgt tcgggttctg cacgctcaaa
    30301 aagctgatta attttagaca ttgagaagac aggctcaata tcaagcgttt ttagtccagc
    30361 cttatcattc ttaatcataa accataaagc gacagcataa gatttaaaaa caccccccgc
    30421 cgtcttatta agatagctac cagcatccgc accatctgtc ttataggggt aaataatcaa
    30481 cgcaagattt tcaagctgat taaaaccttc actcgttccc atgctgacat aatacaaagg
    30541 gttaaagcgg tgcgttaccg gactaaaggg atcaaattta taaacctcat gacctaaaat
    30601 caccttcctg ataagactgg tgatattaaa acactccctt tttggatcga gaataataac
    30661 actccaccag tacttcataa gattagtgat gactatacct gcacctttac cagcccttgt
    30721 tccggctccc atcgcactga atgctgctcc cacatagcgt atgattttct tgtcttttat
    30781 accaacgata atcccgtctt tttcgtcacc gtcaagcgtg aaactgttag actcacgaat
    30841 atcattagtt gtcgcaaatc gcgcatcacc aaaaatatta tctttcttct cgttcagttt
    30901 tatcaacgcc gcaacaggga taataagaga cacgacaaat cctgctccaa gtgcaacaaa
    30961 ggctaaaact ctgatatccg tagtcacaac agataaagta aggctgtctg taaaaatatc
    31021 catcctgtac gcatcaatgg atctgaacac agccttcaat ggtttcagat tctttatctg
    31081 gctgaagtaa agcacaaaga aaataaatga acctgcaaac catgccgcaa caagcataac
    31141 agcgaatata aaaacagatt gcgacttatt aaaagttttc acattcagcc cctttgtaat
    31201 agatgtcgct catatgcctg acaacaccat ccctgcctac atgaacaaca atatcaatac
    31261 tgtccataat gattttacga agaacatcaa aaggaagatt ttgacattct ctgttctgat
    31321 aacattttgt caccatctga atgatggcat cttttgccga ggccgcgtga atggacgtca
    31381 tgcttccact gtgaccagaa ccagcaactt tcacaaaatc ccaggcatcg ccacctttaa
    31441 tttccgccag taaaattctg tcaggtttca ttctcaaaca ggatttgatt aatttcgctg
    31501 ctgttatgat agagccggaa tcactgccgg attccgaagg gtaaaaaaga tgcacataat
    31561 ttttatgaat aaaaaatttg atttcctccg catcttcaat cgtaatcagc cgcgtattca
    31621 atggtatgaa atcgatgaga ctcttcatat aggttgtttt accggaaccc gttgctccgg
    31681 caaatacaat ggttttccct gctgctacgg caagctccat aaaagcagcg atatttctcg
    31741 ctctgaataa atcaagaagc tcatcatcgt atgtcctgtg tttttttcct gactggacat
    31801 aatcataaaa accgttcgcc acatattctt tatgcgttat ctgccgtgtc gatggtttac
    31861 gtatagtgat tgaaatagta tttctctcac atgcaggagg aaaaacgacc tgtacacgtt
    31921 ctcctgattc cagtgttgct gaaagtaatg gcttggtatc gccgatttca tctccccgaa
    31981 atgatgccag cgtctgagaa aaacgtttac aaaaatcaaa ggtcacaccg tcattattat
    32041 ggaatgtcca gtcaccatta atttccgtcc agatttcacc aggacggttg acagcaattt
    32101 ctgttagccc ttcaatatca agaaaagact ggaatacttg atttttataa aaatcgagcg
    32161 atttattgct aatgctgaca ggaataacgc cactcatact attttacctt taactcatag
    32221 atatcagaaa aatcaatgtc ttccccgacc ataatagtga ttatgtcacc ttggttttta
    32281 tacattgttg gaggaatatt tatcgaattc tcaagtgcaa ctttagccat ttcagccatt
    32341 gcctgtcgtg agttttcggt ataatccaca ttacgatctt taccaggtgc attatccgcc
    32401 gccgccgccg ctacatcctg aaccatactg agcataagag aaccaccaaa acgctcccag
    32461 aagtgagaat cgatccatcc atcaatgcct gcttccccca ggggaccagc cgctgctgta
    32521 tttaccatat cgattcgttt attatcaggc gtccgcaact gctcccacat aacgaacatt
    32581 ctgccctgtc catgattcag cgttccggtc tggtaacgac caaaagcctt agttccctgt
    32641 tccagtaatt ttgtgtggtg attagcactc catacatctt ctgtaaaaac acaactgata
    32701 cgacctgcaa catcagaaac aaagcgtgtg gttaaagcac acggtataag cctgttttcc
    32761 tcaatataga gatcaggatc gagattaatc gcagtcactt tcattcctga tttaccaggc
    32821 acattttctt ttgattcttt cctttcttcc tgtggctctg atgtctgcga tgtcctgctg
    32881 ctgccactcg cgccagtctg actccctccc tgaccagcaa caggtattga caaatatttc
    32941 ctgaacccct cctgtagcac ttctttatga ccgtcagaaa cagtattacc agaaccagta
    33001 gcgcctggat tgataattgc tttgttctca acagggtcaa aattctgacc aagaccagta
    33061 cggattttac ttacctgaga aatgctggta tcgcctgttg attctgatgc tttctgctgg
    33121 tcatctgcaa aataattttt atatgttttc agcgcaaaaa taccaataac taatacagcc
    33181 atcagaccaa acagaaagcc agaacgcata cgattatttt tgttcctgat atcaccgata
    33241 tcatgaacgg gtttactttc cagctttgac tgattcgcat catcaactaa atcatctgtt
    33301 tttttcatat cagtcattat tcacctcaac acgttctacc gcaggagaag atgtattcct
    33361 ctggtcggtt aatacttttc cgaaagattt attcacaaca ccaacaacgc tgtttccata
    33421 gcgtaaaaca aatttgtcat taacgttgtg gataaccatg attttatact tgccttttgt
    33481 ttccacatta aaagcaagtg tctgttcctg accatctctt acagcaaatg gagctgggaa
    33541 tactttccct gagttaaaac ctaaaaaggt aaatatgcca ttatcataag caaaatcagg
    33601 ggcgatacga cgactgtcat atttttcatt gactcgcatg aaataatccc agtttttagg
    33661 cgcatcggct ttttcaaaac tttcagaaat cagtttcttc tgtctgagtt tttcctgttt
    33721 ttcttttgcc agccttactt cttctgcatt ctttttcgct atttctgatg gatagcggaa
    33781 ttgtacaaca taagcaggtt gcgcttgttt ttcttctgac agaagg
//
