LOCUS       NZ_CP146999             8159 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain NXQY01 plasmid unnamed7, complete
            sequence.
ACCESSION   NZ_CP146999
VERSION     NZ_CP146999.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39526338
            Assembly: GCF_037126195.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Cui,D.
  TITLE     Emerging Patterns of Antimicrobial Resistance: Concurrent Presence
            of mcr-1.1 and mcr-8.1 in an ST15 Klebsiella pneumoniae Isolated
            from Poultry in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8159)
  AUTHORS   Cui,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-FEB-2024) Veterinary medicine laboratory, Lanzhou
            Institute of Husbandry and Pharmaceutical Sciences of CAAS, No. 335,
            Jianguyan, Qilihe District, Lanzhou, Gansu 730050, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. DECEMBER-2020
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 110.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037126195.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 19:07:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,024
            CDSs (total)                      :: 5,896
            Genes (coding)                    :: 5,728
            CDSs (with protein)               :: 5,728
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 70 of 168
            Pseudo Genes (incomplete)         :: 100 of 168
            Pseudo Genes (internal stop)      :: 30 of 168
            Pseudo Genes (multiple problems)  :: 28 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP146999.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8159
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="NXQY01"
                     /host="Gallus gallus"
                     /db_xref="taxon:573"
                     /plasmid="unnamed7"
                     /geo_loc_name="China: NingXia"
                     /collection_date="2020-04"
     gene            complement(join(7725..8159,1..561))
                     /locus_tag="VYJ08_RS30075"
                     /old_locus_tag="VYJ08_30075"
     CDS             complement(join(7725..8159,1..561))
                     /locus_tag="VYJ08_RS30075"
                     /old_locus_tag="VYJ08_30075"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_338842855.1"
                     /translation="MKFILKKSVVLGSSAIGITLLFIACFLSIQFGRYISLSDRISLTG
                     KTELENKLDEYMNDSYINEATNKVSELSGKLIMQGGNAASPESIQETKVKTEVLSKDKD
                     LDYNSYDSEFLGKKRVITYINLLSVEDSIYKILYPNVDCLGKTFSGTFEGDKPKSKKEC
                     ESEVIKIHKKDAENQRNTIAKIKEINDFITGKIKPLKDEVIANGGAVKIWMPGPSIDTC
                     SEHTAMASEDIPEAYKTKNECISAINKKIKDIDEENARNEGVISFFPFIVILVAILLMP
                     AALKISSLIIKAALNLWLVFLEKSSESINKSEKKIKIDAKIEMREKKDKE"
                     /name="hypothetical protein"
     gene            complement(1153..1554)
                     /locus_tag="VYJ08_RS30080"
                     /old_locus_tag="VYJ08_30080"
     CDS             complement(1153..1554)
                     /locus_tag="VYJ08_RS30080"
                     /old_locus_tag="VYJ08_30080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009310048.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1554..1745)
                     /locus_tag="VYJ08_RS30085"
                     /old_locus_tag="VYJ08_30085"
     CDS             complement(1554..1745)
                     /locus_tag="VYJ08_RS30085"
                     /old_locus_tag="VYJ08_30085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_102025546.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKIHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2214..2363)
                     /locus_tag="VYJ08_RS30090"
                     /old_locus_tag="VYJ08_30090"
     CDS             complement(2214..2363)
                     /locus_tag="VYJ08_RS30090"
                     /old_locus_tag="VYJ08_30090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            2921..3064
                     /locus_tag="VYJ08_RS30095"
                     /old_locus_tag="VYJ08_30095"
     CDS             2921..3064
                     /locus_tag="VYJ08_RS30095"
                     /old_locus_tag="VYJ08_30095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_338842856.1"
                     /translation="MHEPPVQSDRCALSGNCRLESNPERHDKTPLAAAIGKSRCARYLE
                     ST"
                     /name="replication initiation protein"
     gene            complement(3305..4002)
                     /locus_tag="VYJ08_RS30100"
                     /old_locus_tag="VYJ08_30100"
                     /pseudo=""
     CDS             complement(3305..4002)
                     /locus_tag="VYJ08_RS30100"
                     /old_locus_tag="VYJ08_30100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            complement(4233..5240)
                     /locus_tag="VYJ08_RS30105"
                     /old_locus_tag="VYJ08_30105"
     CDS             complement(4233..5240)
                     /locus_tag="VYJ08_RS30105"
                     /old_locus_tag="VYJ08_30105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131685.1"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_338842854.1"
                     /translation="MNIKSALLSAAMAAVSVNCFADTPAPDRHVQQFLDALNGGGGKLI
                     EQLSPQAARQVLIDAQKGATLPAADVSEKTISVDGLNITLKIVKPHNAQGTLPVFMFFH
                     GGGWILGDFQTHERLIRDLVSQSGAAAVYVNYTPSPEAHFPVAINQAYAATRWVAENGS
                     EIGVDGKRLALVGNSVGGNMVAAVALQAKQHKSPAIRYNVMLWPVTDANFDTGSYNQYQ
                     NGYFLSRNMMKWFWDAYTTKESERNNILASPLRATREQLKGLPPTLIQTAEFDVLRDEG
                     EAFGRKLDAAGVDVTVTRYNGLIHDFGLLNALSDVPAVRAAIGQAAGQLKEHLK"
                     /name="alpha/beta hydrolase"
     gene            complement(5457..6224)
                     /locus_tag="VYJ08_RS30110"
                     /old_locus_tag="VYJ08_30110"
     CDS             complement(5457..6224)
                     /locus_tag="VYJ08_RS30110"
                     /old_locus_tag="VYJ08_30110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020050724.1"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_065808205.1"
                     /translation="MSKFAASLAIATALLSASAVAETAKPTVVLVHGAFADSSSWNGVT
                     RILQKDGYNVVAAANPLRSVSTDAAYISSVVNNIKGPVVLVGHSYGGQVITNAARETKN
                     VKSLVYVAAFAPDAGEAAAELAGKFPGSTLGEALAAPVKLADGNVDLSIEQSKFHQQFA
                     HDVSAENAALMAAGQRPVTEAALTEKSGAPAWKTLPSYFIYGDSDKNIPVKSLRFMAER
                     AGSKHTVVIKGASHVVMVSHPQDVATLIEEAAK"
                     /name="alpha/beta hydrolase"
     gene            6377..6844
                     /locus_tag="VYJ08_RS30115"
                     /old_locus_tag="VYJ08_30115"
     CDS             6377..6844
                     /locus_tag="VYJ08_RS30115"
                     /old_locus_tag="VYJ08_30115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014884107.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_065813429.1"
                     /translation="MTKDINTGDLLPDVHGMLCFSLYSAGHAFTQLYRPMLDKLGLTYP
                     QYLVMVTLWNQDGRTVKELSGILFLESSTLTPLLKRLETAGLITRTRNPKDEREVLIHL
                     TEKGNRLKIEASNITRCIVDTLEMDADVVADIKASVDKIRDQIHRAHKPDI"
                     /name="MarR family transcriptional regulator"
     gene            7396..7620
                     /locus_tag="VYJ08_RS30120"
                     /old_locus_tag="VYJ08_30120"
     CDS             7396..7620
                     /locus_tag="VYJ08_RS30120"
                     /old_locus_tag="VYJ08_30120"
                     /inference="COORDINATES: protein motif:HMM:NF020291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="WP_235891633.1"
                     /translation="MQACYERASAYNLQFAPPLDENEVDGIAKSIAKWTISKFSEDQFM
                     DFVKATHTPEIQSKRGKKGGVKSRRAVGK"
                     /name="primase C-terminal domain-containing protein"
     Typing          complement(2357..2571)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.273"
                     /coverage="100.0"
                     /evalue="7.38e-63"
     Typing          2518..3030
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.786"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttctttgatt ttagcgatag tgtttcgttg gttttcagca tcttttttat gtatttttat
       61 tacttcagac tcacattctt tttttgattt tggtttgtca ccctcaaatg ttcctgaaaa
      121 tgttttacct aagcaatcaa cattcggata aagaatctta taaatgctat cttccacaga
      181 caataggttt atataagtta tcactctctt cttgcctaag aattctgaat catagctgtt
      241 gtaatcaaga tctttatcct tagacaaaac ctctgtttta acttttgtct cttgaatgct
      301 ttctggtgat gcagcgttac ctccctgcat tattaattta ccggataatt cgctaacttt
      361 atttgttgcc tcattgatat aactgtcgtt catgtactca tctaacttat tctctaattc
      421 agtcttacca gtaagactaa ttctgtctga gagtgagata tacctcccga attgaattga
      481 taaaaaacaa gctataaata gcaaagtaat acctattgcc gaggaaccta aaacgacact
      541 tttctttaga ataaatttca tttttagaag aatccaacag aagggaaacg ataaaccatc
      601 ttaacatctg atcgcgtgga ccagaacaag cctatacata catcgccccc cttcctgaac
      661 gtgctgaaaa ccggttttcc ggctgcgccg gcaaccggtt ttcagcacgt tcaggaaggg
      721 ggttcgtcga cggccggcaa tgacgttatg gcaagtcgcc ggacgactca ccacacgatt
      781 caccggcgat tcgactcgtg acttgactct agagtcgcca aggtcaaaaa aacaccaaaa
      841 tgacgattcg actctagagt caagtcgtca taagttactg atggtcttaa gtgagtcgtc
      901 gatgactcta tgacgagtcg tcggcaagtc gtacacgaat cgccgacgac ttggattcac
      961 cttacagaaa gtaatactgc gtaatttaat aacccacaac taactgatat taatgagtta
     1021 tcttcaaaat tttaattttg cgtggggtgt gggtattttt agtggtgagg agcgttcaga
     1081 gatctgacga tttttgggaa ggttcaggaa ctccccggaa tgaccggaga ggaaaactga
     1141 agcatgacgg ggtcaggatt ttttccagaa tttccaccat ggcgccgggg attcagttaa
     1201 ttttgcctgg tgattttcaa gaaggagcat ggcctgtttc aggctgtcaa tgtgcgaatc
     1261 cttggacaaa agtaaggtgt tcaggtgttc aatctcctgt ttcagatgac gaatcacctg
     1321 ttcactctgg tcagcagagg ctgtatcacg ctgttcagag acctgtccag aatggtgttc
     1381 acccttcggt tgctcaaagg gaccaaagac acgtaaaagc tctgaagtat ctaccatttt
     1441 tgcattacgt tcaccgctac aggtggacag gtcacctgct ttaattaacc tgtgcagtgt
     1501 tgttctactc ttacccgtca gtcgggcagc ctcactaata gacactctgg ccatcagtta
     1561 ccatcctgta atgcatccag tctcgaggac agtgtacgga aaagatgctc cgcatcatcg
     1621 tggattttct cacaaaggtc cgcctcaaca tcgagatcaa gctcgttaag cttctccagc
     1681 aggagcagag actgattctg gatgaattta gccatgttca gcgctgtttt ctgttgtttc
     1741 gtcataaggg ccctcagagc atcagtgctc agtgtaaacc ggtaaggggg tcggatgtac
     1801 gaatggttca gagaacggat ctgaggtaat cctgtagtta ttttgtagtt tatctgtagt
     1861 catcgcacta aaaatcagga ttaaatcatc aacttttgta gtttacctgt agtccggaaa
     1921 aataatccgt tcatgcaagc atgaacgaag cagttaaaga cgctttttcg tcgccgatgc
     1981 tccttaacct gcgcagatca cgcggccaga atgataattt tttgcccctt aaatggtcat
     2041 acacctaacg cagccgcgat tttcatcgca actgcatgaa ttaaaaggta aaaactggaa
     2101 tctccgttta tttatgtaat acgcaacgta ttacaatcac ctgtaattac atgaaaataa
     2161 agaaataaaa tgtaataccg gcgtattacc acgtacgcgg gcaagcgaaa aagctataac
     2221 gcttcccggc cgacaggcga cccaaaatcc acaggggcat gcaggttctc ttcagtaacc
     2281 tgcgccatta aatcaaaaag gtcatattcg ccgggaagct gcgtatcagt cgaatccagt
     2341 tgttcatcat attcagatgg catggggtga tatccagtag ctgagaggtt gttagtgggt
     2401 ttcagggcgc agccctgaac cagtcacgta gcgctagcgg agtgtatact ggctcaatat
     2461 gttacggcgg agtaacattt aatgaaagtg cttcatgtgg caggggaaat gcagccgcgg
     2521 ggcgtacggg agaaaatgga ggttacggtg atatgacgcc tcctcgctca ctcggtcgct
     2581 tccgctcctg ccgttcggct gcggcgggcg ttacgtgcac acggataaga gcaaaggcat
     2641 atgacatgac aagcagggca ggaaaaggat gcggcgctgc cgtttttcca taggctccgc
     2701 ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga
     2761 ctataaagat accaggcgtt tccccctggt ggctccctcg tgcgctctcc tgttcctgcc
     2821 tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tatctcattc cacgcctgac
     2881 actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca
     2941 gtccgaccgc tgcgccttgt ccggtaactg tcgacttgag tccaacccgg aaagacacga
     3001 caaaacgcca ctggcagcag ccattggtaa gagtcgatgt gcgagatatt tagagagtac
     3061 ttgaagtggt ggcctgagtg cggctacact gaaaggacag ttttggtgac tcgtctcgca
     3121 caagacagtt acctggttaa gcagttcccc aactgacttg accttcgatc aaaccacctc
     3181 cccaggtggt tttttcgttt tcagagcaag agattacgcg cagaggaaaa agatctcaag
     3241 gagatccttt tcagatcgat gaatacacca ttatttcatc gacagtagcg gtaatgactc
     3301 caacttactg atagtgtttt atgttcagat aatgcccgat gaccttgtca tgcagctcca
     3361 ccgattttga gaacgacagt gacttccgtc ccagccttgc cagatgttgt ctcagattca
     3421 ggttatgtcg ctcaatgcgc tgagtgtaac gcttgctgat aacgtgcagc tttcccttca
     3481 ggcgtgattc atgcagcggc cagccatccg tcatccatac cacgacctca aaggccgaca
     3541 gcaggctcag aagacgctcc agtgtggcca gagtgcgttc accgaagacg tgcgccacaa
     3601 ccgtcctccg tatcctgtca tacgcgtaaa acagccagcg ctgacgtgat ttagcaccga
     3661 cgtagcccta ctgttcgtcc atttcagcgc agacaatcac atcactgccc ggttgtatgc
     3721 gcgaggttac cgactgcggc ctgagttttt taagtgacgt aaaaccgtgt tgaggccaac
     3781 gcccataatg cgtgcactgg cgcgacatcc gacgccattc atggccatat caatgatttt
     3841 ctggtgcgta ccgggctgag aggcggtgta agtgaactgt agttgccatg ttttacggca
     3901 atgagagcag agatagcgct gatgtccggc agtgcttttg ccgttacgca ccacgccttc
     3961 agtagcggag caggaaggac atctgatgga aatggaagcc acgcaagcac cttaaaatca
     4021 ccatcataca ctaaatcagt aagttggcag cattacccgg atacgaacaa ggggcccccc
     4081 ccccccgggg atgcggacac tcccatacct ctcgggggaa aactaacaaa actgaaggcg
     4141 ctgtcccggc aaatccggaa cctaatcagg atgacaagca gatggccgag ggttcagcga
     4201 ccagccggcg gtaccggctg gtcacttgtt cgttatttca gatgttcttt cagctggccg
     4261 gcggcctggc ctatggctgc ccgtacggca ggaacgtcac tcagcgcatt cagcaaacca
     4321 aaatcatgga tcaagccgtt ataccgggtc accgtcacat ccactcccgc tgcgtcgagc
     4381 ttacgtccga acgcttctcc ctcatcacga agcacgtcaa actcggcagt ctggatcagt
     4441 gtgggtggta agcccttcag ctgctcacgc gttgcacgca gtggtgaggc gagaatatta
     4501 ttacgctcgc tctctttggt cgtataggca tcccagaacc acttcatcat gttccgtgac
     4561 aggaaataac cgttttggta ctgattataa gaaccggtat caaaatttgc gtcagtgaca
     4621 ggccataaca tgacattgta gcgaatggca ggtgacttat gctgttttgc ctgaagcgct
     4681 acggcggcaa ccatgttacc tcccacgctg ttaccgacaa gggctaaccg ttttccatcc
     4741 acgccaatct cgctgccgtt ttcagccacc cagcgggtag cagcatatgc ctgattgata
     4801 gcaaccggga agtgcgcttc tggtgacggg gtgtagttga catagaccgc tgctgctccg
     4861 gactgactga ccaggtcacg aataagacgc tcatgtgtct ggaaatcgcc cagtatccag
     4921 ccaccaccgt ggaagaacat gaaaacaggc agagtgcctt gtgcgttgtg aggcttcaca
     4981 attttcagcg taatattcag gccatcaaca ctgatcgttt tttcgctgac gtccgctgcc
     5041 ggtagcgtgg cgcctttctg cgcatcaatc aacacctgac gtgcggcctg gggagataat
     5101 tgttcgatga gctttccccc gcctccgttt aatgcatcca ggaattgctg gacatggcga
     5161 tccggtgcag gtgtatcagc aaaacagttg actgaaacag ctgccatcgc ggcagacagt
     5221 aatgccgatt tgatattcat tgtgttacct cattaacgtt gcttgcaatt aaattgcaaa
     5281 ctattcatat gattaagaaa taatctgtat caacacttta ataaacattc ctgtcatctc
     5341 ccatgtgaat gccatctcac cagtaagggg tattcacatc accgaaacag gcaggggcgt
     5401 aagggaaaaa gacagggccc ctgcgggaaa tggagttaaa gacaggacaa agacgtttat
     5461 tttgctgctt cctcaataag tgtcgctacg tcctgaggat gagaaaccat cacgacatgg
     5521 gatgcccctt tgatcacaac ggtgtgtttt gatcccgcgc gttcagccat gaaacgcaaa
     5581 cttttgacag ggatattttt gtcgctgtca ccgtaaatga agtaagaagg cagtgttttc
     5641 caggcgggag cccctgattt ttcagttaat gctgcttcgg tcacaggtct ttgtcctgcc
     5701 gccatcaatg cagcattttc agctgacacg tcatgagcga attgctgatg gaacttcgac
     5761 tgctcaatgg acagatcgac gtttccatca gccagcttaa ccggtgcggc aagcgcttcg
     5821 ccaagcgtgc ttccggggaa tttccccgcc agttcagcgg ccgcttcacc cgcgtcagga
     5881 gcaaatgcgg cgacataaac cagtgactta acattttttg tttcccgggc tgcattcgtt
     5941 attacctgac caccatagga atgcccgaca agtacaacgg gtcccttaat attgttcaca
     6001 acagaagaga tgtaagccgc atcagttgaa acactgcgca atggattagc cgctgcgacg
     6061 acgttatacc cgtctttttg caggatccgg gtgacaccat tccagctaga agagtcagca
     6121 aaggcaccat ggaccaggac aacagtgggc tttgcggttt cggcgactgc actggcagac
     6181 agcagagcgg tggcaatggc gagggaggca gcaaatttag acataatagt ttctctcttc
     6241 aacggttgta tgtttgcgcg caatttgttt gtgcacgatt tgtttgtatg caaattgaga
     6301 tagtactcct ttttgtcgtt gtcaatcgct tgcaattaaa tcgcacacaa attattttag
     6361 aggcactgga ggacatatga ctaaagacat caataccgga gatttactcc cggatgtgca
     6421 cggcatgctc tgcttctctc tctactccgc gggacatgct tttacgcagc tctatcgtcc
     6481 aatgctcgat aagctgggat taacctatcc ccagtacctg gtgatggtca cgttgtggaa
     6541 tcaggatgga cgtactgtga aggaactgag tggaatactc ttccttgagt ccagcacgct
     6601 gactcctcta ctcaagcggc tggagacggc gggcttgatc acccggacac gcaatccgaa
     6661 ggatgaacgt gaagtgctta ttcatctcac agagaagggg aacagattga aaattgaggc
     6721 cagcaatatc acgcgctgta ttgtggatac ccttgagatg gatgcggatg tggttgctga
     6781 catcaaagca tcagtcgata agatccgtga tcagatacat cgtgcacata aaccggatat
     6841 ctgatgatgc tataaagcaa tatggcccta cataacatta agttataggg gcgtatggag
     6901 ctgcatgaca aggtcatcgg gcattatctg aacataaaac actatcagta agttggagtc
     6961 attaccggaa gttttttttt aatgcggcat tttttgggac tattagtgtg ctgtttgtat
     7021 agcgcgagaa gctcaggtcg agacctagaa cctcaaatgt aataatgcat ttaccataac
     7081 atcccttatc agcaccgcct gagaggctct cagggcgttt ctgacgggaa accggtaaaa
     7141 caggcaacaa accacccgaa aacccgccag aaacgcgcag actctgtttt tacggcatgc
     7201 atgagtatgc acgcaagtgc atagcgcgaa aattcacgcc attcagccgg ggtaagtcct
     7261 gtttccgtcg ttcttcctat ccgaaaacgg tgaacgggga cgcaatttat tgcgtggccg
     7321 tgaagcgttg ccggtgcagc cggaaagtgg gcataccgca caattcgtca gggctggctt
     7381 gagtacgacc aatggctgca agcctgctat gaacgtgcca gtgcctataa tttacaattt
     7441 gcaccacctc tggatgaaaa tgaggtagat ggaatagcga aaagtattgc taaatggacc
     7501 atctcaaaat tttcagaaga tcaatttatg gattttgtga aagccaccca tactcctgaa
     7561 attcaatcta aaaggggaaa gaaaggcggg gtgaaaagtc gaagggcggt aggaaaataa
     7621 acccacgaag tgagagacaa agtaaaccgt ggtgttcgat ggggataagt agaagtattt
     7681 attataagtt gaagtcgggg atttaatttc caaccccgac aaatttactc tttatctttt
     7741 ttttctctca tttctatctt tgcatctatt tttattttct tctcagattt atttatagat
     7801 tctgagcttt tctctaaaaa aacaagccag aggtttagtg cagctttgat tattagtgag
     7861 gatattttta acgcagcagg catgagaagt attgccacaa ggattacaat aaaaggaaaa
     7921 aaactaatca caccttcgtt tctggcattt tcttcgtcaa tatcctttat ttttttatta
     7981 attgccgata tgcattcatt ttttgtctta tatgcttcag gtatatcttc agatgccatt
     8041 gcagtgtgct ctgaacaggt gtcgattgat gggccaggca tccatatttt tacggctccg
     8101 ccgtttgcta tcacctcgtc cttaagcggt tttatcttac cagttataaa atcattaat
//
