LOCUS       NZ_CP146685            10122 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain MS 196-1 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP146685
VERSION     NZ_CP146685.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40241992
            Assembly: GCF_037094625.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10122)
  AUTHORS   Tsai,M.T.
  TITLE     Epithelial relay of microbial signals coordinates intestinal
            macrophage supported barrier repair
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10122)
  AUTHORS   Tsai,M.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2024) Immunology, Memorial Sloan Kettering Cancer
            Center, 408 EAST 69st Street, Z-1619, New York, NY 10021, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 192.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037094625.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 18:24:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,468
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,019
            CDSs (with protein)               :: 5,019
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 101 of 320
            Pseudo Genes (incomplete)         :: 209 of 320
            Pseudo Genes (internal stop)      :: 59 of 320
            Pseudo Genes (multiple problems)  :: 45 of 320
            Pseudo Genes (short protein)      :: 4 of 320
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP146685.1.
            Bacteria and source DNA available from Dr. Gretchen Diehl.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MS 196-1"
                     /isolation_source="Colon"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA"
                     /collection_date="2019"
     gene            complement(340..795)
                     /locus_tag="V9Z39_RS27060"
                     /old_locus_tag="V9Z39_27060"
     CDS             complement(340..795)
                     /locus_tag="V9Z39_RS27060"
                     /old_locus_tag="V9Z39_27060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_000787081.1"
                     /translation="MKNEKQAKWLTDRVQTIRGLKSPNEPQRLLVILHDKADKTAQEIK
                     TLSLLIQAERAAEKAQEARTKVMNLIQAEKRAEAKAARKARDHALYQSAGLLIMAGLVD
                     SQTGKPVDDPAKLLGALVSLHELSRDNPKWEEWKLKGQVLLNQKKED"
                     /name="conjugal transfer protein TraD"
     gene            961..2055
                     /locus_tag="V9Z39_RS27065"
                     /old_locus_tag="V9Z39_27065"
     CDS             961..2055
                     /locus_tag="V9Z39_RS27065"
                     /old_locus_tag="V9Z39_27065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_000179383.1"
                     /translation="MASYHLSVKTGGKGKAASHADYIAREGKYAREKDNDLEHQESGNM
                     PAWAAHKPSEFWKAADTFERANGCTYREIEIALPREFTPAQRLELVRDFVQQEIGDRHA
                     YQFAIHNPRAAIEGGEQPHAHIMFSERLNDGIERDPQPYFKRANTKNPERGGAKKVRFG
                     ETPTERKAYLIAQRERWADLQNKHLERYQHIDRVDARSLKAQGIEREPERHFGAGQVRQ
                     FEPAQLHALMERREAERQAQHCRDECNSVIDVTTTLREALSERDNLTLKHEQTSEPQLK
                     ATSDKAFDFEKESDKLNALVSDAMKDIQEEIDLQTLVNDAMAEFQGIHQDMVKAEQQRQ
                     QEKERQRLAEQKRQKSDKGWSFSR"
                     /name="MobA/MobL family protein"
     gene            2068..2652
                     /locus_tag="V9Z39_RS27070"
                     /old_locus_tag="V9Z39_27070"
     CDS             2068..2652
                     /locus_tag="V9Z39_RS27070"
                     /old_locus_tag="V9Z39_27070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039052609.1"
                     /translation="MSDEIKKIAALIGHQQAQEKRVAALIEEFQAESAKLSRQTAQLAQ
                     VIHELDSASGKMTDTIRRSVNAALTQVEKELKQTGLAQQKPAVNALNQVVTAANESVSA
                     MRREMSRYTWKSAIYIALTIFAVMGGCLWGMYYFIDSGYDRIAKMQRMEAVWQKKAPLA
                     DISTCDGKPCVKVDTRQSYGDKKDTYLIIKQ"
                     /name="hypothetical protein"
     gene            complement(2854..4080)
                     /locus_tag="V9Z39_RS27075"
                     /old_locus_tag="V9Z39_27075"
     CDS             complement(2854..4080)
                     /locus_tag="V9Z39_RS27075"
                     /old_locus_tag="V9Z39_27075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080803.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="WP_001080803.1"
                     /translation="MNRLFSNAFWLMASQIGGLLIPLIELPLLARRLGADEYGKVVYVL
                     AISIMASVFIEYGFNFSAVKKIAGRKDDKYISQISTDVFLAKIILSIIPIILALTLIYT
                     TSSGISIPNNWFVWIILFILSFGFTPFWYYISIGNIIFPAILDLLLRVTGLILIVIFVN
                     SPSDAKTTIAIQSMVGALNTLISTAVVFYLSGVGAINIKRALNEIKDGFSVFLYKSSQG
                     IMITISSSLLGFFGGAKSVGFFVPSEKVTKVITTLMITMLNVAYPHFVKNLSDSKNNRR
                     HIKKTILNISAILLFLSLIFSFSVFVFSDKIIFFVFGIEYERAIPLIKTIIWVIPLRSW
                     VLSLTILWFIPINKEKFITKITLVNIMLISFTACLFIYLFGTIGIVMSILLSEVITFVI
                     LTYFFFKEN"
                     /name="oligosaccharide flippase family protein"
     gene            4252..5367
                     /locus_tag="V9Z39_RS27080"
                     /old_locus_tag="V9Z39_27080"
     CDS             4252..5367
                     /locus_tag="V9Z39_RS27080"
                     /old_locus_tag="V9Z39_27080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000538728.1"
                     /translation="MGVNSDHVVTVYTLFSCTLLGFYWLIKRKSFIKRSHIFLLCLVVA
                     YLVINIFVYNHKSITLITSHAVYIVISMWFIFFFKRKPIHLSYLIALGLLFVSSFLLIK
                     LFQLGFGDWGRLTVPVFYDNSWSYFPLGYESSSDPNVLAFMLCIGSIYMFFTLPNKYTI
                     NIIFILSMMCSFLTLSRSSFLSFFISFLIVSVYSFFVLKKTQLRLLVLIMISLAMSFVV
                     MKNIPAVVTENNGASFVTEPNITERIVSKASNSDRIARLKNAIVEISNLKTMFIGNGIG
                     YSTLTQDPHNFYLSTIIDSGILILAIVMMVLTKPLLMVSRTPTKSNVILGCMFLTIYYM
                     FISLFYWQVRVLYFVIILLFTFVNISKKSEQ"
                     /name="hypothetical protein"
     gene            5364..6173
                     /locus_tag="V9Z39_RS27085"
                     /old_locus_tag="V9Z39_27085"
     CDS             5364..6173
                     /locus_tag="V9Z39_RS27085"
                     /old_locus_tag="V9Z39_27085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788525.1"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_000788525.1"
                     /translation="MKNHKVSIIIPCFNNEKTIERCVLSALKQTHRNIEIICINDGSSD
                     NSWNILKKLSSLYGNVFAFNNEDNSGPSFSRNKGVSLSTGHFLSFLDADDYWHPKKLEL
                     QLSFINDENLDFLGSTCSIGEKNNQEINQEIKKEHLKLKIISFNMMLFKNYFQTPAVIM
                     KRDIFIPFNENQRFSEDYMSWLVIVYNKKNKCGLIYGRDLVFLDKFNFGVSGLSGNLWL
                     MEKWELKNIFNFLLKGKIMAVPAILFSLIKYGRRCALTKKNKGKGNK"
                     /name="glycosyltransferase family 2 protein"
     gene            6173..7306
                     /locus_tag="V9Z39_RS27090"
                     /old_locus_tag="V9Z39_27090"
     CDS             6173..7306
                     /locus_tag="V9Z39_RS27090"
                     /old_locus_tag="V9Z39_27090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="WP_016247943.1"
                     /translation="MKISIIGNTANAMILFRLDLIKTLTKKGISVYAFATDYNDSSKEI
                     IKKAGAIPVDYNLSRSGINLVGDLWNTYLLSKKLKKIKPDAILSFFSKPSVFGSLAGIF
                     SGVKNNNAMLEGLGFLFTEQPHGTPLKTKLLKNIQVLLYKIIFPHINSLILLNKDDYHD
                     LIDKYKIKLKSCHILGGIGLDMNHYCKSTPPTNEISFIFIARLLAEKGVNEFVAAAKKI
                     KKTHPNVEFIILGAIDKENPGGLSESDVDTLIKSGVISYPGFVSNVADWIEKSSVFVLP
                     SYYREGVPRSTQEAMAMGRPILTTNLPGCKETIIDGVNGYVVKKWSHEDLAEKMLKLIN
                     NPEKIISMGEESHKLARERFDANVNNVKLLKILGIPD"
                     /name="glycosyltransferase family 4 protein"
     gene            complement(7568..8741)
                     /locus_tag="V9Z39_RS27095"
                     /old_locus_tag="V9Z39_27095"
                     /pseudo=""
     CDS             complement(7568..8741)
                     /locus_tag="V9Z39_RS27095"
                     /old_locus_tag="V9Z39_27095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612096.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(<8813..8899)
                     /locus_tag="V9Z39_RS27100"
                     /old_locus_tag="V9Z39_27100"
                     /pseudo=""
     CDS             complement(<8813..8899)
                     /locus_tag="V9Z39_RS27100"
                     /old_locus_tag="V9Z39_27100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(8907..9863)
                     /locus_tag="V9Z39_RS27105"
                     /old_locus_tag="V9Z39_27105"
     CDS             complement(8907..9863)
                     /locus_tag="V9Z39_RS27105"
                     /old_locus_tag="V9Z39_27105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293214.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001293214.1"
                     /translation="MSELVVFKANELAVSRYDLTEHETKLILFCVAKLNPTIENPTRDE
                     LTVKFSCSEYARTMGLSYENAWGRLNSATSDLFKRSVELIYPTGAVSKRIFNWTEYAEF
                     NREEQTVTLVFSSYIQPLLFHLKKFIKYNLEHVKAFENKYSMRIYEWLLKELSQRKTHR
                     GNIEISIKEFKFMLMLEKNYPLYAELNRWILKPVTNDLNTYSNMKLTIEKRGRPADTLI
                     FQVELDKQIDLVTELAKDPASKKEDKTIRLTPENRLHEGLKTTLHDALTAKIQLTSFEA
                     KFLSDMQSKYDLNGSFTWLTQKQRTTLEKILAKYGRI"
                     /name="replication initiation protein"
     Typing          complement(8908..9863)
                     /name="rep_cluster_1155"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.582"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          962..2052
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="85.984"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cctgtggata aatcagaaaa ttgccgtcga tttgccgtag ttaaaaggat gtcgcaaaca
       61 acgtttgctc cttttcaaga caaacctaaa acacctaagt agctccctcg ctttgctcgg
      121 gcaacgcgct gcccctttct tttgtctccg accatccagc cacagcgccg ctgttttttt
      181 cgtgtcattg ggttcgcttc gctcacggct caatcaggaa ggtggttgtt tgcgcttctg
      241 tgccatgttt tcagtattag gtgataaatt ccattatcaa accaacaaaa cctcgccacg
      301 gactttctgt ggcgttttag agggtattaa cactgtgctc taatcttcct ttttctgatt
      361 gagtaatacc tgccccttca atttccattc ttcccatttg ggattatcac gggataattc
      421 atgcaagctg actaatgccc ccagtagttt tgcggggtca tctaccggtt tacccgtttg
      481 actgtccact aaccccgcca tgatgagtaa ccccgctgat tgataaaggg catggtcacg
      541 tgctttacgg gcagctttgg cttctgcacg cttctctgct tgaataagat tcattacctt
      601 tgtgcgggct tcctgggctt tttctgctgc ccgctccgcc tgaattaaca atgagagcgt
      661 ttttatttct tgggcggttt tatccgcttt atcgtgcaaa atcaccaata agcgttgtgg
      721 ctcattcggt gattttaaac cacggatagt ttgcactctg tctgtgagcc attttgcttg
      781 cttttcgttt ttcatcttta ccactcatgc tgtttttaga atgaaataat tatgctcatg
      841 attggcaact atggtcaaat tccactacaa tccctatcat cctttttatt gatagggcgc
      901 acttagacgt ttcttcactc cgttcagaaa cggtgcgcgc ttgcagcggg cgaaaacagc
      961 atggcatctt atcatctcag cgttaaaacc ggcgggaaag gcaaagccgc ttcccatgcc
     1021 gattatattg cgcgtgaggg aaaatatgcc cgtgaaaaag ataatgacct tgagcatcaa
     1081 gagtccggta atatgcccgc atgggcagcg cataaaccgt ccgagttttg gaaagccgct
     1141 gacacgtttg aacgtgccaa cggctgtacc taccgcgaaa ttgaaattgc cctgccgcgt
     1201 gaatttacac ccgcacaacg ccttgaactg gttcgcgatt ttgttcaaca ggaaattggc
     1261 gaccggcacg cttatcaatt tgccattcat aaccccagag cggcgattga aggcggtgaa
     1321 caaccgcacg cgcatattat gttttctgaa cggcttaatg acggtatcga gcgtgacccg
     1381 cagccctatt ttaaacgggc taacacgaaa aaccctgaac ggggcggcgc aaaaaaggtg
     1441 cggttcggcg aaaccccgac cgaacgcaag gcatacctta tcgcccagcg ggaacgctgg
     1501 gctgacttac agaataagca tcttgaacgg tatcagcata tcgaccgcgt tgatgcgcgt
     1561 tccctgaaag cacaggggat tgagcgtgag ccggagcgac attttggcgc aggacaggtc
     1621 aggcagtttg aacccgcgca gttacacgcc ctgatggagc gcagagaagc ggagcgacag
     1681 gctcagcatt gtcgtgatga atgtaacagc gtgattgatg ttaccaccac gctacgggaa
     1741 gccctcagcg agcgtgataa tctgacactg aaacacgagc agacgtcaga gcctcagttg
     1801 aaagcgactt cagacaaagc gtttgatttt gaaaaagagt ctgacaagtt gaacgcactg
     1861 gtcagtgatg ccatgaagga catacaggaa gaaatcgatc ttcagaccct tgttaacgat
     1921 gcgatggcgg agtttcaggg cattcatcag gacatggtta aggctgagca acagcgtcag
     1981 caggaaaaag aacggcagcg actcgccgag cagaaacgac aaaagtccga taaaggctgg
     2041 tcattttccc gataaacagg attttgtatg agtgatgaga ttaaaaaaat tgcggcgctg
     2101 attgggcatc agcaagcgca ggaaaagcgt gtggcggcgt tgattgaaga atttcaggcg
     2161 gaatccgcca aattaagccg ccaaacagca caattggctc aggtgatcca tgaattggac
     2221 agtgcctccg gtaagatgac cgacacgatc aggcgatcgg tgaatgcagc cttaactcag
     2281 gttgaaaaag aactcaaaca aacgggattg gcgcagcaga aacccgccgt caatgcgctg
     2341 aatcaggtgg ttacagcagc taatgaatcc gtgagtgcaa tgcgacgtga aatgtcccgt
     2401 tatacgtgga agtctgccat ttatatcgca ttgacgatat ttgcggtgat gggcgggtgt
     2461 ctttggggaa tgtattattt tatcgacagc ggttatgacc gaattgcaaa aatgcagcgc
     2521 atggaagcgg tctggcaaaa aaaagccccg ctggctgata tctccacgtg tgacggaaaa
     2581 ccttgtgtga aagtggatac aaggcagtca tatggtgata aaaaagacac ctatctgatt
     2641 atcaaacaat aaaaaaacgg cgctgaatac gccgttttcc cgtgcaagtc gtcaaagtag
     2701 cgaggtggtt ttgagcggtt catggttagg attagaggag ccattttctc ccagagtcta
     2761 tcactgattt gccacttgtt gcctgccata cgttgccctc agaaattatc gtaattcatt
     2821 atattatacg ataattcctt ttgggatagg ctcttagttt tctttgaaaa agaaataggt
     2881 caatatcaca aaagttatta cttctgatag taaaattgac atcactattc ctatagtgcc
     2941 aaacagataa ataaataaac acgcagtgaa cgaaattagc attatattaa cgagtgttat
     3001 ttttgtgata aatttctctt tatttattgg aatgaaccat agaattgtaa gtgacaacac
     3061 ccatgacctt aacggtataa cccaaataat agtcttaatt aatggtatcg ctctttcgta
     3121 ttcaatacca aaaacaaaaa aaattatttt atcggagaaa acaaaaacag aaaaagaaaa
     3181 aattaatgat aaaaataata atattgctga tatgttaagg attgtttttt ttatgtggcg
     3241 tctattgttt ttgctatcac taagattttt aacgaaatgc ggatatgcca cgtttagcat
     3301 tgttatcatt aatgttgtta taaccttagt tactttttca gatggaacaa aaaaaccaac
     3361 agatttagcc cccccaaaaa aacctaataa tgatgatgat atggttatca ttattccttg
     3421 tgaactttta tacaaaaata cactaaaccc atctttaatt tcattaagcg ctcttttaat
     3481 attaattgct cctactcccg acaaataaaa aacaacagca gttgatatta atgtatttag
     3541 cgctcctacc attgattgaa tggctattgt ggtttttgcg tcactcgggg agttaacaaa
     3601 aataacaatg agaattaaac ctgtcactct tagtaaaaga tcgagtattg cggggaaaat
     3661 tatatttcct attgatatgt aataccagaa cggagtgaat ccaaaagata agataaataa
     3721 aataatccat acaaaccaat tattaggtat agatattccg ctagatgttg tgtatattag
     3781 ggtgagagct aaaataatag gtataatact aagaattatc ttagcaagaa aaacatctgt
     3841 tgaaatttgt gaaatatatt tatcatcttt ccttcctgcg atttttttca cagcactgaa
     3901 attgaatcca tactcaatga aaacagaagc cattatggaa atagctaaaa catatacaac
     3961 ttttccatat tcatcagccc ccagtcttct ggcaagcaat ggcagttcga tcaaggggat
     4021 tagaaggccg ccaatctgag atgccattaa ccagaaagca ttagaaaaaa gtctgttcat
     4081 aaaaaatatc atctgttaaa aatgttgagt gaggcatcat gtttatgata ttatagaaga
     4141 atcttattta caataaaaat aaccagttga atcactatgt gtttttatct atacatttgc
     4201 acttttcctt ttatattgtg gtcagttatg ggagggaata aacaggtggt catgggagtc
     4261 aatagtgatc atgtggttac agtatacact ctcttttcct gtacactatt aggtttctat
     4321 tggcttataa aaagaaagtc ttttataaaa agatcacata tttttctcct gtgcttagta
     4381 gttgcttacc ttgtcattaa catttttgta tataatcata aatctatcac tctaattact
     4441 agtcatgctg tatatatagt aatatctatg tggtttattt tcttctttaa aagaaaacca
     4501 atacatttat catatttaat tgctttaggc ctgctttttg ttagctcttt tttgttgatt
     4561 aaattatttc aacttggctt tggtgactgg ggacgtttaa cggttcctgt tttttatgat
     4621 aactcatggt cttattttcc tttgggttat gaaagttcaa gtgacccaaa tgtgttagct
     4681 tttatgttat gcattggctc aatctatatg tttttcacat tgccaaataa atacacaatt
     4741 aatattatat tcatattgtc aatgatgtgt tcatttttga ccttatcacg gagttctttt
     4801 ttatcttttt ttatttcctt tcttattgta agtgtttatt cattctttgt gctaaagaaa
     4861 acacagctaa ggttgttggt actaattatg atttcacttg caatgtcttt tgtagtgatg
     4921 aaaaacattc ccgctgtagt gactgagaat aatggagctt catttgtcac agaaccaaat
     4981 ataacagaac gaatagttag taaagcaagt aatagtgata gaatagccag attaaaaaat
     5041 gctattgttg aaataagtaa tctaaagaca atgtttattg gtaatggtat tggctatagt
     5101 acattaacac aggatccaca taatttttac ttatcaacta ttattgattc cggtattttg
     5161 atattagcaa ttgtaatgat ggtgctaaca aaaccattat taatggtttc aagaaccccc
     5221 acaaaatcaa atgttatttt ggggtgtatg ttcttgacta tatactatat gttcatttct
     5281 ctgttctact ggcaagtgag agtcctatac tttgttatta tattattatt tacctttgta
     5341 aatataagta agaagagtga gcaatgaaaa accataaggt tagtattatc atcccctgtt
     5401 ttaataacga aaaaactata gaaagatgcg ttctttccgc attaaaacaa acccatagaa
     5461 atatagagat tatttgtata aatgatggat cgtcagataa tagctggaat attttaaaga
     5521 aattaagctc attgtatgga aatgtttttg cttttaataa tgaggataat tcagggccgt
     5581 cattttccag gaataaaggg gtttcactat ccacggggca ttttttgtca tttttggatg
     5641 cagatgatta ctggcatcca aaaaaactag aattacaact atcatttatt aatgatgaaa
     5701 acttggattt tttaggttca acgtgttcca ttggtgagaa aaataaccaa gaaattaacc
     5761 aagaaattaa aaaagaacat ttaaaattaa aaataatttc atttaacatg atgttgttta
     5821 agaattattt ccagactcca gccgtcatta tgaaaagaga tatttttatt ccatttaatg
     5881 agaatcagcg tttttcagag gactacatgt catggcttgt tatcgtttat aataaaaaaa
     5941 acaaatgtgg attaatatat ggaagggatt tggtttttct cgataaattt aactttggag
     6001 tgtcagggtt gagtggtaat ttatggttga tggagaagtg ggagttaaaa aatatattta
     6061 acttcttgtt gaaaggtaaa ataatggcag tgcctgcgat cttgttttct ttgataaaat
     6121 atggaagaag atgcgcttta acaaagaaaa ataaaggtaa gggtaataaa taatgaagat
     6181 ctcaataata gggaacacag caaatgctat gattttgttt agattggatt taataaaaac
     6241 actaaccaag aaagggattt cagtctatgc ttttgctact gactataatg attcatccaa
     6301 ggaaataata aaaaaagcag gcgccattcc tgttgattat aatttaagtc gcagtggtat
     6361 taaccttgtt ggtgatttat ggaatactta cttattaagt aaaaaactaa agaagataaa
     6421 accagatgct attttatctt ttttttcaaa gccctctgtc tttggatcgt tggctggtat
     6481 tttttcaggc gttaaaaata ataacgctat gcttgagggg ttaggttttt tatttacaga
     6541 gcagccacat ggaactccgt taaaaacaaa gttacttaaa aatatccagg ttctcctgta
     6601 taaaataata tttccacata tcaactcatt aatacttctt aacaaggatg attatcatga
     6661 cttgatagat aaatacaaaa taaaattaaa atcttgccat attcttggtg gcattggttt
     6721 agatatgaat cattactgta aaagcacgcc accaacaaat gaaatatcat tcatttttat
     6781 agctcgtttg ctagcagaaa aaggagtcaa tgagtttgtt gctgccgcaa aaaaaataaa
     6841 aaaaacacat cccaatgttg aatttattat acttggcgct atagataagg aaaaccccgg
     6901 agggttatct gaatctgacg tagatacttt aattaaatca ggagttattt cttatcccgg
     6961 atttgtttct aatgtggctg attggattga aaaatcaagc gtatttgttc ttccttccta
     7021 ttatcgagag ggagttcctc gtagtacaca agaagcgatg gctatgggga gaccgatttt
     7081 aactactaat ttaccaggct gcaaagaaac aattattgat ggtgtgaatg gatatgttgt
     7141 aaaaaaatgg tcacatgaag atcttgcaga aaaaatgctg aagttaatta ataatcctga
     7201 aaaaataatc agtatgggag aagaaagtca taagttagca agagaaagat tcgatgcaaa
     7261 tgtaaataat gtaaagttat taaaaatact aggaattcct gattaataaa cgaaaagcgg
     7321 ctctgattca ttcggaacta agaacctatc tcaataggag ctaaattcat gaccttaccc
     7381 agccatatcg atgtttaaaa gtcaggctgt ataaggtttt ggttacagtg ataatatatt
     7441 tttatgcggt aattaatccc tattgagata ggttctaaac tgccgttata tcagtgttag
     7501 agcctattcc aatacaattt tattccagtc gattatgctg tatgcaaaat acaacattgt
     7561 gtagtggtca acaaaaactg gccacggctt tagagttttt caagaacaat cgttctgatt
     7621 cattcggcgt caaaccacca ttatattgat gaggtctgag ctggctgtaa tatcctgtga
     7681 tgtaattcgt tattgccatg ctggcttggc taaaattagc gtagccatta tccggtaccc
     7741 actctgtttt caggcttctg aagaaccgtt ccattgggct gttatcccag caatttcccc
     7801 ggcgactcag actttgcttt atctgatatc tccacagtaa ctgactgaaa ttcctgctgg
     7861 tatagtggct gccttgatcc gagtgataca gtaaattatc cggttttccc cgtgcttccc
     7921 aggccatcga cagggcttta gctgtcagtg ctgaatcagg gaaaaatgac atcgcccagc
     7981 cagctggttt acgggaaaac aaatcgagta ctacaccaga taagcccggc gttttcccgt
     8041 ccagatataa gtcacatcac cgcaccaggc gtgattaggc tcggtaacag caaactggcg
     8101 atccagataa ttagggatct caatgtgttc cttagacgcc ttcttataac gatggacagg
     8161 ttgctgacag ctgataatat tgagcgcttt cattagcttt gttgcccgtc agcggctcag
     8221 ttttatacct ttggtggtga ccatcgcggt aatgcttcgc gctcctgctg agccgttact
     8281 ttcgcgataa acttcacgca caaggctcag taatgccact cttgtggcgt caggcttccc
     8341 gtgctgccgc cagtatttat aactgctgcg atgaacccca aacacgttgc acacaatggc
     8401 aacaggaaac tgcgccctga gtttctcaac taatgagaat tgtttaggga gtctgacatc
     8461 aaggtctcgg tagcattttt taatatatcc ctttccattt caacacgttg aagtcttttc
     8521 tttagctcgc gtatttcaat ctgctcaggt gtcatgggtg aagtagtggg ggattttccc
     8581 gctcgctctt ctttcagttg tcgaacccat ttgtccattg tggatttgcc gacgttcatt
     8641 gccgtagcag cggcggcaac ggtgtaatgc tgatcgagta aaagctgggc agcttcgagg
     8701 cgaaacccgg ggctaaaatt gcgtctgtta cgtccggtca taatgtcacc tgtttttact
     8761 gcgaagtgat gatatcacct ctattcaggt ggccaaattc agtatgccac tacaattttc
     8821 cttgcttaat atagcgatgt attgttgctc tgttttttcc agttagtctg gctgcctctg
     8881 taactgatac cagtgccata gatacctcat atccgcccgt atttagccag aattttctct
     8941 agcgtagttc gttgtttttg agttagccat gtaaatgagc cgttaagatc gtatttgctt
     9001 tgcatatcgc tcaggaattt tgcttcaaaa ctagtcagtt gaatttttgc agttaaagca
     9061 tcatgcaatg ttgtttttag cccctcatga agacgatttt caggcgttaa acggattgtc
     9121 ttatcttctt tttttgatgc gggatctttt gctagttcag tcacaaggtc aatttgttta
     9181 tccagttcaa cttgaaagat cagtgtgtca gcaggacgac cgcgtttttc aatagtcaac
     9241 ttcatattgc tgtaagtgtt taagtcattt gtaactggtt ttaggatcca gcgattcaat
     9301 tctgcatata aagggtagtt tttttcaagc atgagcataa acttaaattc cttgatactt
     9361 atctcaatgt ttcccctatg cgtttttctt tgtgaaagct cctttagtaa ccattcatat
     9421 attcgcattg agtatttatt ttcaaaggct ttaacatgct caaggttgta tttaatgaat
     9481 ttttttaaat gaaataaaag tggttgaata tatgagctaa atactaatgt tacagtttgt
     9541 tcttctcgat tgaactcagc gtattccgtc caattgaaaa ttcgtttaga aactgctcct
     9601 gtaggataaa ttaattcaac agagcgttta aataaatcac tagttgcact gttcaatctt
     9661 ccccaagcat tttcataact taagcccata gtgcgtgcat attcagaaca tgagaattta
     9721 actgttaatt catcccttgt tgggttttca attgtagggt tcaactttgc aacgcaaaac
     9781 agaattagct tggtttcatg ttcagttaga tcataacggc ttactgctaa ttcatttgct
     9841 ttaaacacaa ctaattcaga catgctaatc accattatgt agtaatttaa atcgctacat
     9901 gaatcataat gatgtagcga tttaaatcaa ctacattttt tcgggtttga ttgtgaataa
     9961 cctaattttt cctacatctt tcccactgct agcctaattt ttcctacatc tagcctaatt
    10021 tttcctacat ttaatggttt tttttttatt aaaaaacaat aagttaaaat tattagaata
    10081 aagaaagaat aaataaaaga taaaaaaaga atagatccca gc
//
