LOCUS       NZ_CP146600            11969 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain KP15 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP146600
VERSION     NZ_CP146600.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40200543
            Assembly: GCF_037094485.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11969)
  AUTHORS   Yang,L.Y., Guo,T. and Li,G.C.
  TITLE     Genomic of Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11969)
  AUTHORS   Yang,L.Y., Guo,T. and Li,G.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-FEB-2024) School of Medicine, Yangzhou University, 88
            Daxue South Road, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 08-FEB-2024
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 185.0x
            Sequencing Technology :: Illumina NovaSeq; Nanopore Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037094485.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 18:16:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,979
            CDSs (total)                      :: 5,858
            Genes (coding)                    :: 5,679
            CDSs (with protein)               :: 5,679
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 67 of 179
            Pseudo Genes (incomplete)         :: 121 of 179
            Pseudo Genes (internal stop)      :: 22 of 179
            Pseudo Genes (multiple problems)  :: 31 of 179
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP146600.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11969
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP15"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed1"
                     /geo_loc_name="China:Jiangsu"
                     /collection_date="2022-04-20"
     gene            276..521
                     /locus_tag="V8V92_RS27120"
                     /old_locus_tag="V8V92_27120"
     CDS             276..521
                     /locus_tag="V8V92_RS27120"
                     /old_locus_tag="V8V92_27120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            795..1166
                     /gene="tolA"
                     /locus_tag="V8V92_RS27125"
                     /old_locus_tag="V8V92_27125"
     CDS             795..1166
                     /gene="tolA"
                     /locus_tag="V8V92_RS27125"
                     /old_locus_tag="V8V92_27125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            1163..1528
                     /locus_tag="V8V92_RS27130"
                     /old_locus_tag="V8V92_27130"
     CDS             1163..1528
                     /locus_tag="V8V92_RS27130"
                     /old_locus_tag="V8V92_27130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            1703..1981
                     /locus_tag="V8V92_RS27135"
                     /old_locus_tag="V8V92_27135"
     CDS             1703..1981
                     /locus_tag="V8V92_RS27135"
                     /old_locus_tag="V8V92_27135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            1969..2283
                     /locus_tag="V8V92_RS27140"
                     /old_locus_tag="V8V92_27140"
     CDS             1969..2283
                     /locus_tag="V8V92_RS27140"
                     /old_locus_tag="V8V92_27140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2447..2875
                     /locus_tag="V8V92_RS27145"
                     /old_locus_tag="V8V92_27145"
     CDS             2447..2875
                     /locus_tag="V8V92_RS27145"
                     /old_locus_tag="V8V92_27145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            2901..3080
                     /locus_tag="V8V92_RS27150"
                     /old_locus_tag="V8V92_27150"
     CDS             2901..3080
                     /locus_tag="V8V92_RS27150"
                     /old_locus_tag="V8V92_27150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3107..3637)
                     /locus_tag="V8V92_RS27155"
                     /old_locus_tag="V8V92_27155"
     CDS             complement(3107..3637)
                     /locus_tag="V8V92_RS27155"
                     /old_locus_tag="V8V92_27155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3644..4375)
                     /gene="mobC"
                     /locus_tag="V8V92_RS27160"
                     /old_locus_tag="V8V92_27160"
     CDS             complement(3644..4375)
                     /gene="mobC"
                     /locus_tag="V8V92_RS27160"
                     /old_locus_tag="V8V92_27160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(4375..6339)
                     /locus_tag="V8V92_RS27165"
                     /old_locus_tag="V8V92_27165"
     CDS             complement(4375..6339)
                     /locus_tag="V8V92_RS27165"
                     /old_locus_tag="V8V92_27165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(7823..7972)
                     /locus_tag="V8V92_RS27170"
                     /old_locus_tag="V8V92_27170"
     CDS             complement(7823..7972)
                     /locus_tag="V8V92_RS27170"
                     /old_locus_tag="V8V92_27170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(8057..8314)
                     /locus_tag="V8V92_RS27175"
                     /old_locus_tag="V8V92_27175"
     CDS             complement(8057..8314)
                     /locus_tag="V8V92_RS27175"
                     /old_locus_tag="V8V92_27175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(8324..10009)
                     /locus_tag="V8V92_RS27180"
                     /old_locus_tag="V8V92_27180"
     CDS             complement(8324..10009)
                     /locus_tag="V8V92_RS27180"
                     /old_locus_tag="V8V92_27180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(10114..11601)
                     /gene="ltrA"
                     /locus_tag="V8V92_RS27185"
                     /old_locus_tag="V8V92_27185"
     CDS             complement(10114..11601)
                     /gene="ltrA"
                     /locus_tag="V8V92_RS27185"
                     /old_locus_tag="V8V92_27185"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     BGC             3324..11969
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          7142..7720
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3648..4375)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 acatatacac agtataatta ttaccttact cctcccgttt tcaggtgtca atatatgaga
       61 ttagttctca tcttcaggca caaaaaagcg cccggagcca cctgtaagca gatcgcgtct
      121 tttcccgaaa atttctcatg gcaacaaatt ttattattta agacattcat cacacttaca
      181 tttcagatgg tatagacaca tctgaaagct aactggcatg aggagaggac ctgcggttaa
      241 actgttcggg aaacctgaga cgcccggagg ccactatgac gcacctgaaa ctgactcccg
      301 ctgaagaaaa gcgcctggcg gacgcctttg tcgctcaggc actggccatg ggcctgaaag
      361 ggaacaaaac gctgccaaag gcggtaaaac aacaggtgcg ggaaagggtg cttcgtgcca
      421 ttcaggacga acgtgaagcc aggcgggcgc aggaaatgcg tcgggagaaa tacgaggcta
      481 cggaaaccac cttcacctgg cagccacagc gccgccggta aggggtattt accataacgt
      541 cgcattgccc gcaccgctca gacggtctga gggcgtttcc ggcggctacg ggataaaaca
      601 ggcaacaaac cacccggaaa accgccggaa acgcgcccgg taaattgtaa cccgctgcat
      661 gcctatgcac gcaactgcat ggcgggccgt ttggtgtaaa aacgatacga taagccgtgt
      721 ttatgtaaat gaacgtggcc gtaaagagtg acaaatatac atgtaacatt ttttactgac
      781 aggtcatcaa cccgatgcgt ttaataccct ttatcctgct ggcattgtgc agccccttac
      841 tcctgacggc ctgtaagcat tccaccaccc agccgccgaa aatttatact ctggataagt
      901 catctaaaat cagtagctac gctgcggcca tcaagaccgc tgttgaaatg cagatgtttg
      961 aaccagaggc ctatgcggga aaaatgtgca ccgtgcggat atccatacaa cgtgatggca
     1021 ctctgaacag cgccattgct gaaggaggag atccgaaact ctgtaaagca gcaatatcag
     1081 ccgttaccca cgccaaaatt ccgccggcac cggatgaaaa tacctggcaa atattcaaaa
     1141 acgcccctct ggatttcaga ccatgaaagc ttttactctg acagtgctgg cggccatgtt
     1201 cctcactccg gtacatgcga taccactaaa acccgtaacc acactgcagc caacctaccc
     1261 ggtacaggcc gcagcaaaac gcatcgaagg tcaggtgacc gccacgtttg atattctgga
     1321 cgatggacga gtggacaacg tgtcgcttac cggctgtcac cttttttttc gggaaacgct
     1381 gacggcaatg aggcactggc tatatcccgc agggcacccg gcaatgcgac aggttataaa
     1441 ttttgaattc agtctgaaaa atgacggctt acaggatccg cgaccagtca ccacccaaaa
     1501 gctcaatgat gatggttgcc gccagtaaca ggtcctttaa cccgcaggct gcaatagtcc
     1561 tggatgatga gtgagcctgt agcagccctg attagtggaa gcgaagctga aaaatgggca
     1621 gcaaaagccg tatttctggc aaatagcgcc gccacggatt aaagtagtca tgtgtaatac
     1681 atatgactac aaaggggctt ttatgtcatc agcaacatcc atcaggctcg atgaagaact
     1741 taaggaccgg ctgaaaacgc tggccgatga ccggcaccgc tcagcgcacg cgctaatgct
     1801 ggaggcaatc accgaataca tcgaccggga agagaagcgc agccagtacc tgcgggacgg
     1861 acaggccgca tggcagcact atcaggaaac gggtctgcac cttactgcgg aagaagcgga
     1921 agcctggata agtacctggg gaactgagaa cgagcaggat gctccgccat gccacaggta
     1981 acgatttccg cgctggcaca gcgcgattta caacgccttc aggactttct caaaaccaaa
     2041 aatcggctgg cagccagaaa aggaggtgag gtgatcgtcc gggctatcca gcaactgaaa
     2101 acattgccag acattggccg cccggtgccg tttctgccgc tggaatataa ggaactggtg
     2161 atcgggtttg gcgacagtgg ctatgtgatg ctctaccgcc acgaccggga aatggaccag
     2221 attgtgatcg tcaccgtcag gcatcagaaa gaatccgggt atccgggggc agacagcctc
     2281 taataaccga tgacgggggc gcaattcatt gcgtggccgt cgccggtttg acaagcgcag
     2341 cgcgtcagaa aaccacatcc tgaacggaaa aacgggtaaa attcagcgat acatttaccg
     2401 tgagaaatta cacacctttc acacccgata aaatcagggg tatacagtgg cgaaaacatt
     2461 gctcaacctt tcacaggcag cacaagccgc gggcattacc agaagaacgc tatacaatca
     2521 tgtaaaacag ggaaaggtta ccgtgtcacg tgacgggaaa aacaaccctg tcgtggatgt
     2581 gtcagaactg atccgtgtat acggaaatgt gaatatccct gagaaacaaa ttcccggcat
     2641 ttcacaccgt gaaaatacac aaaaaaattt cccacaggaa cagcttctgg caatgcaaaa
     2701 ggaactggcc gatctccggc aggctgtcac cctgatgctg gaagataaaa catcccggga
     2761 agaagaacgc cgccagcacg atgatgagcg ccggaaactc caggccgaag tggacagatt
     2821 gacaacagaa ctgacacaga agaaaaaacg cttctggtcc ggctggttta gctgagaact
     2881 taccgaaggg aatggagaac atgacgaagc aggaaaaaac tgccctcaat atggctaaat
     2941 tcattaagga tcagtcgctg ctgttgctgg aaaagctgaa cgagctggac atggataccc
     3001 cggccgacat gtgcgaacgg cttcacgagg atgcggaaac actctggcag gtgatgcggg
     3061 ataagctcgg ggaagaataa cagcaccggc catgccggtg ctggcgttat tttttgccga
     3121 acggccacca ccggcgggcg ggtttgtcct ccggcagttc aggcaccggc acgttaaaat
     3181 agcggctgtt atattcccgc atcagctccg ctttcagctt ctcgttcacc gcgctttccc
     3241 cgtgccgctc gctgaccacc tcaatcatcg cgttaatctg ctccggtgag cacttcccgg
     3301 catcaaccac ctgcacaccg ttctcgatgg cgaaccgctc cacctttcga tagaggtaat
     3361 cacacagctt cattcctgtg ggtttgtccc gttcccattg cggattttca ccggtgaaaa
     3421 agtacgtggc tttttcgtcg ctgttgagcc gctcgtcata catcccgatg gatttcatca
     3481 tcgccagatg ctgggcccgg tcgacaaaac agaaaacata cgccccggtc tgccaggcga
     3541 gcaccaccat gtcttccaga tgctcgctgg ttggcataat gcagcgctcg ccgtctttgt
     3601 cttccacgtc ctcaataaat accatgtaca gcgccataat ccctcactcc gttgtcataa
     3661 atttaaagca cgcgaacagc ctctcccggt ccagctccac cgttttgcgg gcctcgccgg
     3721 tctgtggatt cacgatttca tccaccgcca gaatcgcccg ctgtacccgg acaagcgtcg
     3781 gccggtcagg gcagatgtac cagatttcat cgatgccgtt cgcccggcgg ttgaacagat
     3841 acccggacac cacttcctga taccgcctgc ggctcttgac ggtgcgctct acctcaaatg
     3901 ccaccgttct gccgtccagc tccgccagcg cgtccggcac tttcatccct ttcagggcca
     3961 tacggtgcag gtaccgccag ccggtggccc cccggccctc catggagagg cgcagggact
     4021 gcacggccag ccggtgcggc agctgcgccg ccgacaccct tgacggctga aacggaataa
     4081 cgtccgtcag cggctcgtcc tcgtcgaacg agaacgccac cccgtgcggc gtcagccccc
     4141 acacgctgac ctgacggccg tcaaccgggt atttcgcccg gaccagcagc cctttcgcca
     4201 ccagactgtc caggaagcgg taaaccggct gtaccgccgc aatccccagc cgtcgcgcaa
     4261 ccagctccgc cgtggaacac agctcttccc gcagccagcg cagggtcagc cgtgttttct
     4321 ccgcgttccg cgcctgccgc ttcgcgtggc tgacgttgta ctgctccagt gtcatcagta
     4381 aagccccgca aaatcatcat ccgcttttgg tccgccgccg tcggccggag gcaggtactc
     4441 cgccagcggg tccgttttcg ccagcaccgg gaaggttttc agcgaaattt cccggcgctc
     4501 aacccgcagc gggctgctga acgccagccg cgccacgccc agcccggtca tcaccgcgaa
     4561 cccgtcaggc aggtgttgca gggtgttcac gtcaaacagc ggccgcgcct ccttctggat
     4621 gaagctgtcg ccgctgatgt gctccacgtt ccccgcctcc cggctggccc ggcggcgctc
     4681 cacgtccacc cggatctccc cggtctggcc cgccgcccac tgcgcggact cctggctggc
     4741 cgcgcgatag aaccagcgga tcggcgtgtt atccagtacc gtggttttca caaaatcagc
     4801 gggcagatcc tgcccgcact gaccgaagtc gcccagcgac tggtgcgcga gcagcaggtt
     4861 gcagtcccgg ctgcgcagcg tccccagcgc gttcagcacg tacttcgaca gcagatattt
     4921 aatctcatcg agcatgatgc tcgcgtgcgg ccacctccgg aactcatccc gcgcaatgat
     4981 gatttgcgcg cagcgggcaa acagcatctt ctgcacccgg atcaccgcct catcgtccat
     5041 cgagccgatg acgtacaggc agccgccgcc gttgattatc cccgccacat cgataccgct
     5101 gtccgtctgc aggacgctca ggtcggccac gttctccagc ttggtgatca gccccttcac
     5161 gtcctttttc agggcctccg gcaacgcgta cgccgccgcc agcacctgct gaatgtttgc
     5221 cccctgcggg aactgctccg caatcagctt cgccgctttc tgctcgctga tgcggtagtg
     5281 gtccgccacg tcgccggtct cactcaggtt aaacccggca accagcagat tcttcagggc
     5341 gtactggtcg agatcgcgga acagattcag ctgcgccggt ttccccggcc gcagatccag
     5401 cagggtgaag tccgggcagt gcgcccggaa gacgtgcggc gcccacgcgt cccctttggg
     5461 gtcgaagtac accacagcgt ccccgaaatc gcgtacgcag cgaatgagcg cgttggtggc
     5521 catcgcgctc ttgccgctcc cgggcaggcc gaggatctga atattggtct tgcgccaggt
     5581 atcacgaggg accagcaccg gctggccctg ctcatcctta cccagaaaca ccgcctgctg
     5641 tttccgggcc tgctccgtgg aaaaatcggc cagcgcgtta accggtaccg gctccccgaa
     5701 atgcaggtgg cggacgtctg tcatttctcc ggtccgctgg ctgctgcgcc ggctctcctg
     5761 attcagtttc gcgttcaccg cctcgtcagc ggccgcaaaa tgaccatcaa gcagcaggaa
     5821 cgccagcagc cgcgccagaa ggacgccggc gacaagccct ccggcacacc aggctatccc
     5881 gaacaggtta gccggctgca gcaggtaata atctgcgttc gcatagaggt ccgggcgcag
     5941 gacggtttct ccccagctct cgataaacac ggcgacaacc agccccccca gaagggccag
     6001 accggcagcg ccggtcagcc agtacgcgta gttccagaca tgtcgccgtg ccctgaccat
     6061 ccgcccgggt gaatccagcg aggccagcac cgaaaatggc acagtccggt tattatcgcg
     6121 cttatagcga tcggcatact cccgccgcgc cttatggctc agccccacaa aacgcattcg
     6181 gccggcccag agttcagcca gaccgaaaaa caggccgata accagaaaaa aaccggccag
     6241 actgtaatac tcccggtaca gccagctgac ccacgggtaa atctgccggg ccagctccgg
     6301 gatatgccac tgccaggcaa gcgaatccgt attaaacatg caatctctct ccttcagacg
     6361 acgggtttac cggcgtgatg cgcagccccc gctgcacaag ccgcggacat gccccaaggc
     6421 atggcggcgg cggtcacgtc cgtggtgatt gcgttcagct ttcaccgcgg aggccagcct
     6481 ctttccgccc catgcggaaa aggcgcagcc ccggaggaac aacgagcacg ccccagcgtg
     6541 tgagaggaaa aggcggcacc ccagtgcggt cttttccggt gttccggccc ccgtcagagt
     6601 tcgccatagc gagcggctga ccacccgcaa aatcaggagc agctttgctg cttcgtcagg
     6661 tgcgtccagc accgtggatt tttgtgggtg gtcaggcgtg agcctgacgg gggccgggtg
     6721 aatttctggc gtggccggcg tccagccgga cagagccaga aagaggggaa aagaccgtgc
     6781 gtcggtggcg tccagccagc gtaagcaagg tcttttcccc tgaagtcagc cacgccccag
     6841 cggggatgac ttgcatttct ccgcaaggag aaaaaagcaa cagagatagg ttaacgtaaa
     6901 atgttcagcg aaaaaccgac atggttcacc tatcctgata attgatcgtc agggaataga
     6961 aagacttaat caggggacaa tatcccacat cagcgcgtcc ggtgaccgtc ttgcaggtgc
     7021 cggggaaatt gcgcctcctc gctcactcgg tcgctacgct ccgggcgtga gactgcggcg
     7081 ggcgctgcgg acacatacaa agttacccac agattccgtg gataagcagg ggactaacat
     7141 gtgaggcaaa acggcagggc cgcgccggtg gcgtttttcc ataggctccg ccctcctgcc
     7201 agagttcaca taaacagacg ctttttcggt gcatctgtgg gagccgtgag gctcaaccat
     7261 gaatctgaca gtacgggcga aacccgacag gacttaaaga tccccaccgt ttccggtagg
     7321 tcgctccctc ttgcgctctc ctgttccgac cctgccgttt accggatacc tgtccgccgt
     7381 tctcccttgc gggaagtgtg gcgctttctc atagctcaca cactggtatc tcggctcggt
     7441 gtaggtcgtt cgctccaagc tgggctgtaa gcaagaactc cccgttcagc ccgactgctg
     7501 cgccttatcc ggtaactgtt cacttgagtc caacccggga aagcacgcaa aacgccactg
     7561 gcagcagcca ttggtaactg ggagttcgca gaggatttgt ttagctaaac acgcggttgc
     7621 tcttgaagtg tgcgccaaag tccggctaca ctggaaggac agatttggtt gctgtgctct
     7681 gcgaaagcca gttaccacgg ttaagcagtt ccccaactga cttaaccttc gatcaaacca
     7741 cctccccagg tggttttttc gtttacaggg caaaagatta cgcgcagaaa aaaaggatct
     7801 caagaagatc ctttgatctt ttctactgaa ccgcgatccc cgtcagttca gaggaggacg
     7861 atggtgccac cgttccgccc tgaacatccc ggatataatt tgcctgacat gcggccagaa
     7921 gaaagccagc aacaatcaat ataaaaagaa aaatagtctt tacctttttc atatactttc
     7981 cctgtattta aagactccgg cattcatccc tgaacaccgg aagtcagtat ttcaaatcat
     8041 ccttgataat tttttgttac cagttaccgt cccgataatc aaaagctata taacaattat
     8101 ccacatcagg ttttatttga tttttaaaaa atggctgtag taaaggaacc cattctttct
     8161 tcaaatcaaa ctcaccatta ttaatattat cctttaaagg cattcccaag ctttcaatga
     8221 cagaaccatc gtcaccaaag tctttagaat actcttcccc tttaaactct tcagttttct
     8281 tatcaaacca tgtcaaacgt aatttaagcc ccatatttac ctctcaaaga tattttttaa
     8341 tgttccgttt tggatctggc cctttaacct gcttacccgt tttgggatca aatgcgccta
     8401 ggtgttcgcc atcactggca cgataccctt caagctcacc gtgctgggaa tcccattcat
     8461 aaattttacg ttttttatcg ccataccatc gggcccgctt accaccacca ccttgtttag
     8521 gtgttttagg atcacctcgc tttaactctc caagtccctt tatgtcttct gttttaggta
     8581 ctggatgata atcatggccg tagtctttag tccccttgcg aggcttgttc ttttcctcat
     8641 ttaacttatt ttctgcatcc ttcgcttttt tctccttttg tttccggctc tccatcgccc
     8701 cggtaagtgc agccttatct ctggataagg attcattcat tgcgtttaaa gatgcctgag
     8761 ccgcttttaa tttctcattc tgattctgaa tatccttatc aagatagcga acctgatcct
     8821 gccacccgga gccatattca tgaggcgggt tataagtcac aaaatcatct ttatttgcct
     8881 ctgcctcttt taccttttta tcggcttcct gaacagcagc ccttgcaccg gggagcgcct
     8941 tctctgtcga tgcaatgcgg ctgtttaagg tcgcaatatt tttatcttcg gcatccagct
     9001 cagctttcgc tttatcatag tctcttttca gcccctcttc cggatgagcg gcgtcccatg
     9061 cctgctggcg acgcttttct tcctcctgac gctgttttac ctgtgccggg gtaagaacat
     9121 ctgtcaccga cacataaacc ggcttctgtc cggtctcttt cgggaaacga ataacggcct
     9181 cacgggagtt accaccggct gtaaaaccag ccggacgtga atcacctttt tcagcaacaa
     9241 tgccttttgg cggggctttc gctgtcggaa cacctttcgg tacgctcacc tgcagagacg
     9301 ggagacccgg aatcgacaca ctgaataccc ccggacgttt tgtcggtttc gcatccacca
     9361 cagggacact cattggccga cctgcgacaa ccgcaatatg ctgccgctca tccttcacca
     9421 catccacaac ccgttgtctg acacgaaccg tcgcctggtc cagtggtaaa gtacttgccg
     9481 gagtctccgt caccgcatcg gccggtaatg acgtcataat ttttgacatc attttcggat
     9541 catcttttgc aatctcagaa ggcagcacac cgtagatcgc aatcccccac agaccaaact
     9601 taaacggccc cttcagggca gccagcacat cagcaacggc ggccgacaac gcatcgccgg
     9661 aaactgataa agccagtcct tcagcccccg gagtagccag ggcaggcaga ccgaaggcca
     9721 tcgcagtggc cgaagactgt ccgccaccag agctaccgct atgattgccg ctgttaccac
     9781 cattaccacc gggtttactc ccgccgttac caaactcccc cggattatag ttatgaatat
     9841 ctccattcgg cgtatgcgtc gtaccccagc ccggcccgct gtttggatcc gtaccaccac
     9901 ctgatgaagg gccaccttta ccagacgtac cgttcacatt cccactggcc tgaccaccat
     9961 tatgtcccag acctgaatta cccggacctc gaccgtctcc accactcata ccgttttcct
    10021 ctttgacatt taaaacaata aattaaagcc aacatgtcgc ggtagggata cccgttaccg
    10081 gatacccccc gcacagatcc cggcgtgcgc gatttacgca ccgggctcct gcctcgggtg
    10141 tctggcggtg aaccgctcca caggccatgg atgaagaacc cgaacccttg gtagccatgc
    10201 ggctgccagt ttgtttgctt tcgtccaggt cgtatcatcc ttctggctcc tgcgcctgag
    10261 cgcccggcgc cagaggtttg ttacgtgtgt cctgaacttc tgcatggtgg ggaagttgcc
    10321 cggtaccgag tgatagttca ggtatccctg aaccactctc ctgagccatt ttccctgttc
    10381 ggggattgag taatgccagc gccttcgcag accgtctttg atggctttca gagttgccgt
    10441 catccgatcc cggcgggtct ttcgtatcag catgaacctg ccgttgcgat ctttcccgct
    10501 gatgtgcgtg aacccgagga agttgaacgt ttctggtttg ccttttcccc tgatggcacg
    10561 gttttcggca gcgaagcggc cgaactccat cagacgggtt ttctccgggt gaaccgtgag
    10621 tccgaactcc ctcagtctgc gctgcatggc tatacggaag cgccgggcat cgtatcgttt
    10681 gtcgaacccg atgacgatgt catcggcgta tctgaccatt accacattgc ctgtggcata
    10741 gcgacgtcgc cactgatgcg cccacagatc gaagacgtag tggaggtata tgtttgccag
    10801 cagcggtgag atgaccgcac cctgtggggt gccttcctcc gttgctcgcc attgaccctc
    10861 ctccgacgtc ccggctgtga gccacttacg tatgagcctg attaccctcc ggtcgccgat
    10921 ccgatgctct gtgaacctga tcagccattc gtggctcacc ctgtcgaaga actgactgat
    10981 gtcggcatcc agtacccagt ttacgttagt gcgtaccagc cctgtggcca gtgcgtccag
    11041 tgcatcgtgc tggcttcgcc cgggtctgaa cccgtatgag aaccccataa agtcgttttc
    11101 atagactgcg ttcaggattt tcaccagcgc atactggacg atcttgtcct ccagcgaggc
    11161 gatgccgagc gggcgttgtt ttccatccgc ttttgggatg tagtgacgcc tgccgggctg
    11221 cgccctgtag ctgccctgat gtagcctccg gtgcagatct gttatgttgt tcttcatgtt
    11281 tccggcgtag tccatccacc tgatgccatc cactccggcg gccgctttcc tgctcaggga
    11341 gaggaatgcg gcttccagtg cttcgactgt cagcaggtgg aacaatgctg taaaccgttc
    11401 tttcttccgc tgcttcgcag cttcccgcac gcgtgacagc ctctgtgaca tgctttcccg
    11461 gctctgtgtc cggcgcatgt gtggctgttc cgcgttcccc ttggccccgc tccttcgctc
    11521 cactgactcc gctcctttcg ggttgttcgc ctgcttcgcc gctactatga gcgagtccga
    11581 cttctcctct ccgtacatca ccggctatga ctcctcgtct tcccggtgcg ggccatctcc
    11641 gacactggca gatggtcaga ggggagatct cccggttccc gcgtagagat cgtattgaca
    11701 tgccagggtc tcagaccccg ccgggtccat gtggcactcg cagtatcgca ccctatgatg
    11761 ttgccttccg ttaacagtac aacgtcggca cccggtaatt taatatacat ttcgtggctc
    11821 aatggctggc ctgtcaacac ccctgtcaac gcttcgcccc atacctcgcg gtatgcaacg
    11881 catgactcgg ggaccttgtg gattgctggt ccttcaatgg tcggggactt tcaccccttg
    11941 atctctaacc ggtctcccgg cgcacactg
//
