LOCUS       NZ_CP146573             5631 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain B1.H1.1 plasmid pB1.H1.1.c1, complete
            sequence.
ACCESSION   NZ_CP146573
VERSION     NZ_CP146573.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40199718
            Assembly: GCF_037081875.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Rolbiecki,D.
  TITLE     Genomic and metagenomic analysis reveals shared resistance genes and
            mobile genetic elements in E. coli and Klebsiella spp. isolated from
            hospital patients and hospital wastewater at intra- and inter-genus
            level
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Rolbiecki,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-FEB-2024) Department of Water Protection Engineering
            and Environmental Microbiology, Uniwersytet Warminsko-Mazurski w
            Olsztynie, Prawochenskiego 1, Olsztyn 10-720, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: GS De Novo Assembler v. Flye 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 342x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037081875.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 18:26:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,233
            CDSs (total)                      :: 5,114
            Genes (coding)                    :: 4,790
            CDSs (with protein)               :: 4,790
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 180 of 324
            Pseudo Genes (incomplete)         :: 157 of 324
            Pseudo Genes (internal stop)      :: 35 of 324
            Pseudo Genes (multiple problems)  :: 45 of 324
            Pseudo Genes (short protein)      :: 4 of 324
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP146573.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B1.H1.1"
                     /isolation_source="Hospital Wastewater"
                     /db_xref="taxon:562"
                     /plasmid="pB1.H1.1.c1"
                     /geo_loc_name="Poland:Olsztyn"
                     /lat_lon="53.77 N 20.48 E"
                     /collection_date="2021-04-20"
                     /collected_by="University of Warmia and Mazury in Olsztyn"
     gene            complement(175..408)
                     /locus_tag="V8956_RS26135"
                     /old_locus_tag="V8956_26130"
     CDS             complement(175..408)
                     /locus_tag="V8956_RS26135"
                     /old_locus_tag="V8956_26130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137437.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(415..945)
                     /locus_tag="V8956_RS26140"
                     /old_locus_tag="V8956_26135"
     CDS             complement(415..945)
                     /locus_tag="V8956_RS26140"
                     /old_locus_tag="V8956_26135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<983..1681)
                     /locus_tag="V8956_RS26145"
                     /old_locus_tag="V8956_26140"
                     /pseudo=""
     CDS             complement(<983..1681)
                     /locus_tag="V8956_RS26145"
                     /old_locus_tag="V8956_26140"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1623..1946)
                     /locus_tag="V8956_RS26150"
                     /old_locus_tag="V8956_26145"
     CDS             complement(1623..1946)
                     /locus_tag="V8956_RS26150"
                     /old_locus_tag="V8956_26145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_046072959.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLCQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2011..2202
                     /locus_tag="V8956_RS26155"
                     /old_locus_tag="V8956_26150"
     CDS             2011..2202
                     /locus_tag="V8956_RS26155"
                     /old_locus_tag="V8956_26150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165975.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3405..4271)
                     /locus_tag="V8956_RS26160"
                     /old_locus_tag="V8956_26155"
     CDS             complement(3405..4271)
                     /locus_tag="V8956_RS26160"
                     /old_locus_tag="V8956_26155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(4273..5325)
                     /locus_tag="V8956_RS26165"
                     /old_locus_tag="V8956_26160"
     CDS             complement(4273..5325)
                     /locus_tag="V8956_RS26165"
                     /old_locus_tag="V8956_26160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001053268.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     Typing          complement(2297..2378)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          complement(2835..3022)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          complement(72..1633)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcaaccag atgctacagg ccacattagc ccgccttaat ccactgaaca cgtcattttt
       61 catggtttca ccatcccagc gaagggccat ccagtgtccg ctcctggatt tccggctgac
      121 gctcccgctc cagagataac tcatgttcgc gttcctgcat cagccgttca tcccttatct
      181 cctgttctcg ctgtataact ggctcaagcg ttctgtccgc tcgctcaagt gctgcacctg
      241 ctgactcaac tgcatgactc gcccgttcag catcgcgttg tcccgttgcg taagcgaaaa
      301 cattttctgc aattccacga aggcgttctc ccattcgttc agccgctgca tatagtcctg
      361 ttgcagttgc tctaatgcgt tcagcaaatg tctttccagc tccgtcatgc tctgtcactc
      421 ctgcaccagt ctcactgcgt tcagtttgcc gttgtccttc gtccagttcg tttcggcttt
      481 catccccttc ggcaacacca gaaaacgtcc gttgctgtcc tcgtgataac gcacgcccca
      541 tgtcttcgcg ttcagcttct ccagactctc tttctgctcg cggagaatat tcatattcgc
      601 gtttatcttc atgccctggt accacagcag actcccggtc gtggcaaaca tcagtactga
      661 gacgataatc agccacagcc agcgtttcac gctcattttc agtacggttt tccagtgcgc
      721 gtccatcgta tcgacggtcg ttttctgata cctctccagc agttctttca gggctgttgt
      781 atggtcgttg atagcgttcc tgattaaaga ttcgtttgag gtcagttccc tgtttacaga
      841 ttcttcgagc ttcctgaact cgtcgttcag cattttctct gtagattcgg ctctctcttt
      901 cagctttttc tcgaactccg cgcccgtctg taaaagattg ctcataaaac gctcctttca
      961 gtctgatatt cctcccgccg ttcgggtctg caatgctgat actgcttcgc gtcaccctga
     1021 ccacttcaag ccccgcctct gtgagcgcct gaatcacatc ctgacggcct tttatctctc
     1081 cggcatggta aagcgcgtct atcccgcgtg tgacgccctc agcaagcgcc tgtttcgttt
     1141 ccggcaggtt atcaggaagg gtcagcgtcc ggcggttctc cggggcgttg gggtcatgca
     1201 gaccgtaatg gtgattcacc agcgtctgcc aggcatcaat tctcggcctg tctgcccggt
     1261 cgtagtacgg ctggaggcgt tttccggtct gtagctccat gttcggaatg acaaaattca
     1321 gctcaagccg tcccttgtcc tggtgctcca cccacaggat gctgtactga tttttctcga
     1381 gaccgggcat cagtacacgc tcaaagctcg ccatcacttt ttcacgccct ccaggcggca
     1441 gctccttctc cgcgaacgac agaacacctg acgtgtattt cttcgcaaat ggcgtggcat
     1501 cgatgagttc ccggacctct tccggattcc cctgaagcac cgttgcccct tcccggttac
     1561 gctcccttcc cagcaggtaa tcaaccggac cactgccacc accttttccc ctggcatgaa
     1621 acttaactat catcccgcgc cccctgttcc ctgaccgcca gacgcagccg gcgcagctca
     1681 tccccgatgg ccatcagtgc ggccaccacc tgaacccggt caccggaaga ccactgcccg
     1741 ctgttcacct tcctcgctgt ctggttaagg ttattcccga tggcggccag ctgacacagt
     1801 aacggcggtg ccagtgtcgg cagcttcccg gaacgggcaa ccggctcacc caggcagacc
     1861 cgccgcatcc ataccgccag ttgtttaccc tcacagcgtt ccagtaaccg ggcatgttca
     1921 tcatcagtaa cccgtatcgt gagcatcctc tcgcgtttca tcggtatcat tacccccatg
     1981 aacagaaccc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac
     2041 atggcccgct tcatcagagg ccagacatta acgctgctgg agaagctcaa cgaactggac
     2101 gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgccgatga gctttaccgc
     2161 agttgcctcg cacgcttcgg ggatgacggt gaaaaccgct gacacatgca gctcccggag
     2221 acggtcacag cttgtctgtg agcggatgcc gggagcagac aagcccgcca gggcgcgtca
     2281 ggccgttgac ctttggggtg tcggggcgaa gccctgaccc agtcacgtag cgatagcgga
     2341 gtgtatactg gcttaaccat tcggcatcag tgcggattgt atgaaaagtg cgccatgtcc
     2401 ggtgtgaaat gccgcacaga tgcgtaagga gaaaatgcca gttcaggcgc tcttccgctt
     2461 cctcgctcac tgactcgcta cgctcggtcg ttcggctgcg gcgagcggac tctcctttct
     2521 caaaaacgct aaccctgtta tccacagaac ccggggataa accaagaaag aacatgtgcg
     2581 caaaaaacga aaccccggga aaggccgcgc cggaggcgct ttttccatag gctccgcccc
     2641 cctgacggac ttcacaaaac agacactttt tcggtgcatc tgtgggagcc ttgcggctca
     2701 accatgaaat ccgccagcgg tggcgaaacc cgacgtggct taaagatcat caccgtttcc
     2761 ggtgaatcgc tccctcctgc gcccccacgt tcccgccata tcgattaccg gatacctttt
     2821 ccgcctttct cccttacggg acacgtggcg ctttctcata gctcacgtct tctggtatct
     2881 cggggcgact taggcagttc gctccgggct gggctgtatg caggaacccc ccgttcagcc
     2941 cgactgctgc gccttctccg gtaactgttc tcttgggtcc aacccggaaa agacaccaca
     3001 aagcgccact ggcagcagcc attggtaaaa gtcatgtgcg agatatttag agagtacttg
     3061 aagtggctgc cggagtgcgg ctacactaga agaacaattt tggtgactcg tctcgcataa
     3121 gacagttacc tggttaagca gtctgcaagc tgacttaacc ttcgatcaaa ccgccctgca
     3181 aggcggtttt ttcgtttaca ggcagcagat tacgcgcaga aaaaaaggat ctcaagagga
     3241 tcctttgatc tttttgttac aaacgtctct atttgactcc cctgagagcg ttttagcgcg
     3301 cctctgaccc ccttggttac attccgacac cagaaacgct cccgtttttt acgtgcattt
     3361 ggcacggtat cgatggaggc cggggggcaa tcctgaacag aatgttactc ctctgagtat
     3421 tttctgttaa gaaaatatac acttagcaca gttacaataa atcctacccc ctgtaagaat
     3481 gtgaattgtt catccaaaaa caaccatgac aaaaataatg caactagcgg aataaagtac
     3541 aacattatcc ctgccaaatg aactgggagc caacgcaagg agttcaagta aaaacaaaaa
     3601 gctaaaaaat attgtaatat tccagatgaa aaaacaataa tcacggattc caacggaaga
     3661 taaattgatg atttagatat aaaaataaag agcgatgaca gtataaggga gaatgtcaac
     3721 tgtccgctca ataatgtgat aggctcaaac gatttaataa ggcgagacga tataacaaca
     3781 taaaatgctg ccgataacac accggcaaca acaagacaat atccaacaat attattcgat
     3841 ccattactga tgtctggcaa ggctaccaaa atactgccaa caattgcacc tataaggaac
     3901 aatataaaat attgcgggga attaatttta actgagcaaa taagaacaga caacaccaat
     3961 ataaacactg attcaagtga aaaaataatt gatgcgcttc ctgctgaaat aaaataaagt
     4021 ccgtagagtg taaaagtata cgctaagaat ggttcaaaaa cgcctgtcgc ataagcaagt
     4081 atcgcttttg agtttaatgg gagttttttt ctttttataa aaagaactat ccaagatagt
     4141 attactgcac tacaaatctg ccagaaaaac acccctccgg catctagttc ttcaactcct
     4201 tttttgctca ttacgatagc tgccccccat gagaacgctg ctaaaaaagc aaaaagcatt
     4261 cctttaatca tattaatcat cctctttctc ataaaggggt ttgttttcaa atttccttgg
     4321 ttgaaaggag ttaaaaaaca tagatgcatc atctacagga ataccaataa actctccata
     4381 ataaggatca tctataggaa actcttctaa tgaaacataa aacaatacat tccagttgaa
     4441 gatcattgga tcactatttt ctatttcatg gagcaatctt tcatccttag ttaattctcc
     4501 tggaattaat acatcagtat gataaaaacc gctgtaataa gaactattac cggtgtgcac
     4561 tgggcatcca aaacgatcca aattagattc tgaatgccat gttgtagaaa tcccttccgg
     4621 atgtgaatac ccacccttac caacagactg aatagtcatg ttacgaatat ccgctaacgc
     4681 ttgttcttga tctttataga aacctttttc tatcgccagc ctcataaaat gccccggggc
     4741 atttacagaa ataagtattc cccctggtat tcttctatca tgatttcttg ttctatgagc
     4801 agaagagtaa aaaacattaa caccagcctt atatattttt agcccatctt tatcctgaaa
     4861 tggtacgctt tcagtataaa taacatcctc ttttattgga tagcactctg gcatatgcag
     4921 cgaacaaata tatttttgta tgtccactaa ctcttctgat ggacgagcaa aggcaagttg
     4981 tggatgaata aaataaatga gatgcgcact gacctctcct cttcttgcgc gttctttaaa
     5041 acgaactctt tcattacgca aaaaatcaaa caaatgacca tcatcattat atagttgggg
     5101 gctatttaca atagataaat ggaaatcaag ccctttaatt ttcttgcttg ccaatttacc
     5161 aaatacacag ggctgattgc ctgacaccca ttttcttagt aaatcttcaa ttttatcttt
     5221 agaagtttcg ttaaagataa atgaatgaat attatcaata tcttttccaa atgaaaaact
     5281 ggatgataat tgtggtagtt tatttatacg ttgttgaaga ttcatgaaag ctcccacaag
     5341 acacctcata tattttttta tatactgaaa ctacattata tttattaccg gcgtaaaacg
     5401 aaataattta ttatcgttac cgatatttta aaaaaatcat tatttgcaca gtgttcgagt
     5461 tgttagtatc actccttcct gaaaatccgc tccatttacc ataacgtcgc attgtacgca
     5521 ccgccaaaac gcgttcggtg cgatcacggc agcagacagg taaaaatggc aacaaaccac
     5581 ccgaaaaact gccgcgatcg cacctgataa attttaaccg catgaatacc t
//
