LOCUS       NZ_CP146570             4207 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain B1.H1.1 plasmid pB1.H1.1.c4, complete
            sequence.
ACCESSION   NZ_CP146570
VERSION     NZ_CP146570.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40199718
            Assembly: GCF_037081875.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Rolbiecki,D.
  TITLE     Genomic and metagenomic analysis reveals shared resistance genes and
            mobile genetic elements in E. coli and Klebsiella spp. isolated from
            hospital patients and hospital wastewater at intra- and inter-genus
            level
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Rolbiecki,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-FEB-2024) Department of Water Protection Engineering
            and Environmental Microbiology, Uniwersytet Warminsko-Mazurski w
            Olsztynie, Prawochenskiego 1, Olsztyn 10-720, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: GS De Novo Assembler v. Flye 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 342x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037081875.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 18:26:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,233
            CDSs (total)                      :: 5,114
            Genes (coding)                    :: 4,790
            CDSs (with protein)               :: 4,790
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 180 of 324
            Pseudo Genes (incomplete)         :: 157 of 324
            Pseudo Genes (internal stop)      :: 35 of 324
            Pseudo Genes (multiple problems)  :: 45 of 324
            Pseudo Genes (short protein)      :: 4 of 324
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP146570.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B1.H1.1"
                     /isolation_source="Hospital Wastewater"
                     /db_xref="taxon:562"
                     /plasmid="pB1.H1.1.c4"
                     /geo_loc_name="Poland:Olsztyn"
                     /lat_lon="53.77 N 20.48 E"
                     /collection_date="2021-04-20"
                     /collected_by="University of Warmia and Mazury in Olsztyn"
     gene            join(3741..4207,1..589)
                     /locus_tag="V8956_RS00410"
                     /old_locus_tag="V8956_00410"
     CDS             join(3741..4207,1..589)
                     /locus_tag="V8956_RS00410"
                     /old_locus_tag="V8956_00410"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_338775141.1"
                     /translation="MWQARFYQSLPRIVADYPDARWMFLTLTVRNCAIGELGETLNRMN
                     MAFQRLKDRKEFRPVQGWIRTTEVTRGSDGSAHPHFHTLMMVPPSMFTRDYVKHDRWVE
                     LWRECLRVNYDPNVDVRAVKPRKPKDGESLASATAELVRGAVAETLKYSTKPMNMAFQR
                     LKDRKEFRPVQGWIRTTEVTRGSDGSAHPHFHTLMMVPPSMFTRDYVKHDRWVELWREC
                     LRVNYDPNVDVRAVKPRKPKDGESLASATAELVRGAVAETLKYSTKPADMVADPEWFLE
                     LTKQTHKRRFVATGGALKDILKLDQETDADMVIGDDIPEGDDDGSRIAFEWKTESKKYR
                     RSPSKDKAESD"
                     /name="protein rep"
     gene            1248..1550
                     /locus_tag="V8956_RS00415"
                     /old_locus_tag="V8956_00415"
     CDS             1248..1550
                     /locus_tag="V8956_RS00415"
                     /old_locus_tag="V8956_00415"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023150476.1"
                     /translation="MAGSIRARSLYLYLRDNSGDKKWHQALRTKASGLICVVEGFGSIL
                     LGVVALKATPATTAIVRGCDHLQKFDSKRQPTGDTAMITRQMSHRPAWDGVHAKP"
                     /name="hypothetical protein"
     gene            1537..2523
                     /locus_tag="V8956_RS00420"
                     /old_locus_tag="V8956_00420"
     CDS             1537..2523
                     /locus_tag="V8956_RS00420"
                     /old_locus_tag="V8956_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_016245711.1"
                     /translation="MQNPKLQNLTDYSPSDAPWDAHKSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGETLNRMNMAFQRLKDRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPSMFTRDYVKHDRWVELWRECLRVNYDPNVDVRAVKPRKPKDGE
                     SLASATAELVRGAVAETLKYSTKPADMVADPEWFLELTKQTHKRRFVATGGALKDILKL
                     DQETDADMVIGDDIPEGDDDGSRIAFEWKTESKKYRRSPSKDKAESD"
                     /name="protein rep"
     gene            3182..3484
                     /locus_tag="V8956_RS00425"
                     /old_locus_tag="V8956_00425"
     CDS             3182..3484
                     /locus_tag="V8956_RS00425"
                     /old_locus_tag="V8956_00425"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023150476.1"
                     /translation="MAGSIRARSLYLYLRDNSGDKKWHQALRTKASGLICVVEGFGSIL
                     LGVVALKATPATTAIVRGCDHLQKFDSKRQPTGDTAMITRQMSHRPAWDGVHAKP"
                     /name="hypothetical protein"
     Typing          complement(316..504)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.947"
                     /coverage="98.0"
                     /evalue="1.39e-59"
     Typing          complement(2250..2438)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.947"
                     /coverage="98.0"
                     /evalue="1.39e-59"
ORIGIN
        1 aatgaacatg gccttccagc gacttaagga tcgcaaagag tttcgacctg ttcagggctg
       61 gatccgcacc acggaagtta cacgtggaag tgacggtagt gcgcacccgc actttcacac
      121 tctgatgatg gttcctccct caatgttcac gcgagattac gttaaacatg accgctgggt
      181 agagctttgg cgagagtgtt tgcgtgtgaa ttacgatccg aatgttgatg ttcgagcagt
      241 aaaaccccgc aagcctaagg acggagaatc attggcttct gcaactgcgg aacttgtacg
      301 gggcgcagtg gctgaaaccc tgaaatattc cacgaagccg gctgacatgg ttgctgatcc
      361 tgaatggttc ctcgaattga ctaagcagac acataagcgc cgatttgtgg ctaccggtgg
      421 cgcactcaaa gacatactga aactcgatca ggaaactgat gccgatatgg taatcgggga
      481 tgatattccc gaaggcgatg atgatggttc acgcattgct tttgagtgga aaaccgaatc
      541 caaaaagtac cgtcgctcac cgtcaaaaga taaagctgaa tcagactgac agggtgtcgg
      601 ggcgcagccc tgaccacgac gtttttgaga gggcgccgct tgtcggcggt actccaaaaa
      661 cacatcttgt cctattggtt ctactccgat gcaggatagg gctaacgccg caaagtgacg
      721 tcactcaatt actgagttcg gtttccgaac tcagttggga caagatgtgt ttttggtaca
      781 ccgccatcac gatgtcggtg ccaaaaacga ttcgtcaggg ctgcgccccg acaccctgcc
      841 aacacctcac gcagtcagat taaaatctgc cagcgctcgc cgtttccgaa atcgtcaaac
      901 tctaaatcgt ggggtcccgc tggcttgcca gtgggttccg atttagtgat tgacgacatt
      961 gctgattgaa aaattggctg aggtggttgg attcacgaag tgaggccaac cacctggaag
     1021 acggttttta attgtctggt accggataca accagaacga atggagttgg ttctgtcgtg
     1081 atgcgaaaaa gtatgggttc gcaaatcaca gactaaatag atttacccaa ttccattcga
     1141 gtgaggttgt tatgtttttt gaggttctta atcatgttga tagtgcgttg agcgttgctg
     1201 tttcgctcct ggcgttgtgg cagtttttgg tggcgggtgg gcgttgaatg gctggttcga
     1261 tccgcgcgcg atccttgtat ttatacttaa gggataactc tggcgataaa aaatggcatc
     1321 aagccttgcg taccaaggct tcggggctta tttgcgttgt agaaggtttt ggaagtattc
     1381 tgttgggagt ggtcgcctta aaagcgaccc ccgcaacgac ggccatcgtt cgggggtgtg
     1441 accacctgca gaaattcgat tcgaagcgac aaccaacagg tgatacggca atgataacac
     1501 gtcaaatgag ccatcgccca gcatgggacg gtgtgcatgc aaaaccctaa actccagaac
     1561 ctcacggatt actccccctc agacgcgccc tgggacgctc acaaaagcgt ttctgacgac
     1621 gtaggtggga tttaccttct cgcagccgaa tatgagcgct acggggcgcg tatggcttcc
     1681 tgtggcggtt tactgaggtt tggttggtct accctcaaag aaactggaga aactcgcctt
     1741 aggctacgtg aggcgcattt ctgccgtgtc cggcactgtc ctgtctgcca gtggaggcga
     1801 tccctgatgt ggcaagcccg tttttatcag tccctgccgc gtattgttgc ggattatcca
     1861 gatgcacgct ggatgttcct gaccctcaca gtgcgaaact gcgcgatcgg ggaattgggt
     1921 gagacactta accgaatgaa catggccttc cagcgactta aggatcgcaa agagtttcga
     1981 cctgttcagg gctggatccg caccacggaa gttacacgtg gaagtgacgg tagtgcgcac
     2041 ccgcactttc acactctgat gatggttcct ccctcaatgt tcacgcgaga ttacgttaaa
     2101 catgaccgct gggtagagct ttggcgagag tgtttgcgtg tgaattacga tccgaatgtt
     2161 gatgttcgag cagtaaaacc ccgcaagcct aaggacggag aatcattggc ttctgcaact
     2221 gcggaacttg tacggggcgc agtggctgaa accctgaaat attccacgaa gccggctgac
     2281 atggttgctg atcctgaatg gttcctcgaa ttgactaagc agacacataa gcgccgattt
     2341 gtggctaccg gtggcgcact caaagacata ctgaaactcg atcaggaaac tgatgccgat
     2401 atggtaatcg gggatgatat tcccgaaggc gatgatgatg gttcacgcat tgcttttgag
     2461 tggaaaaccg aatccaaaaa gtaccgtcgc tcaccgtcaa aagataaagc tgaatcagac
     2521 tgacagggtg tcggggcgca gccctgacca cgacgttttt gagagggcgc cgcttgtcgg
     2581 cggtactcca aaaacacatc ttgtcctatt ggttctactc cgatgcagga tagggctaac
     2641 gccgcaaagt gacgtcactc aattactgag ttcggtttcc gaactcagtt gggacaagat
     2701 gtgtttttgg tacaccgcca tcacgatgtc ggtgccaaaa acgattcgtc agggctgcgc
     2761 cccgacaccc tgccaacacc tcacgcagtc agattaaaat ctgccagcgc tcgccgtttc
     2821 cgaaatcgtc aaactctaaa tcgtggggtc ccgctggctt gccagtgggt tccgatttag
     2881 tgattgacga cattgctgat tgaaaaattg gctgaggtgg ttggattcac gaagtgaggc
     2941 caaccacctg gaagacggtt tttaattgtc tggtaccgga tacaaccaga acgaatggag
     3001 ttggttctgt cgtgatgcga aaaagtatgg gttcgcaaat cacagactaa atagatttac
     3061 ccaattccat tcgagtgagg ttgttatgtt ttttgaggtt cttaatcatg ttgatagtgc
     3121 gttgagcgtt gctgtttcgc tcctggcgtt gtggcagttt ttggtggcgg gtgggcgttg
     3181 aatggctggt tcgatccgcg cgcgatcctt gtatttatac ttaagggata actctggcga
     3241 taaaaaatgg catcaagcct tgcgtaccaa ggcttcgggg cttatttgcg ttgtagaagg
     3301 ttttggaagt attctgttgg gagtggtcgc cttaaaagcg acccccgcaa cgacggccat
     3361 cgttcggggg tgtgaccacc tgcagaaatt cgattcgaag cgacaaccaa caggtgatac
     3421 ggcaatgata acacgtcaaa tgagccatcg cccagcatgg gacggtgtgc atgcaaaacc
     3481 ctaaactcca gaacctcacg gattactccc cctcagacgc gccctgggac gctcacaaaa
     3541 gcgtttctga cgacgtaggt gggatttacc ttctcgcagc cgaatatgag cgctacgggg
     3601 cgcgtatggc ttcctgtggc ggtttactga ggtttggttg gtctaccctc aaagaaactg
     3661 gagaaactcg ccttaggcta cgtgaggcgc atttctgccg tgtccggcac tgtcctgtct
     3721 gccagtggag gcgatccctg atgtggcaag cccgttttta tcagtccctg ccgcgtattg
     3781 ttgcggatta tccagatgca cgctggatgt tcctgaccct cacagtgcga aactgcgcga
     3841 tcggggaatt gggtgagaca cttaaccgaa tgaacatggc cttccagcga cttaaggatc
     3901 gcaaagagtt tcgacctgtt cagggctgga tccgcaccac ggaagttaca cgtggaagtg
     3961 acggtagtgc gcacccgcac tttcacactc tgatgatggt tcctccctca atgttcacgc
     4021 gagattacgt taaacatgac cgctgggtag agctttggcg agagtgtttg cgtgtgaatt
     4081 acgatccgaa tgttgatgtt cgagcagtaa aaccccgcaa gcctaaggac ggagaatcat
     4141 tggcttctgc aactgcggaa cttgtacggg gcgcagtggc tgaaaccctg aaatattcca
     4201 cgaagcc
//
