LOCUS       NZ_CP146569             3631 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain B1.H1.1 plasmid pB1.H1.1.c5, complete
            sequence.
ACCESSION   NZ_CP146569
VERSION     NZ_CP146569.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40199718
            Assembly: GCF_037081875.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Rolbiecki,D.
  TITLE     Genomic and metagenomic analysis reveals shared resistance genes and
            mobile genetic elements in E. coli and Klebsiella spp. isolated from
            hospital patients and hospital wastewater at intra- and inter-genus
            level
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Rolbiecki,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-FEB-2024) Department of Water Protection Engineering
            and Environmental Microbiology, Uniwersytet Warminsko-Mazurski w
            Olsztynie, Prawochenskiego 1, Olsztyn 10-720, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: GS De Novo Assembler v. Flye 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 342x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037081875.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 18:26:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,233
            CDSs (total)                      :: 5,114
            Genes (coding)                    :: 4,790
            CDSs (with protein)               :: 4,790
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 180 of 324
            Pseudo Genes (incomplete)         :: 157 of 324
            Pseudo Genes (internal stop)      :: 35 of 324
            Pseudo Genes (multiple problems)  :: 45 of 324
            Pseudo Genes (short protein)      :: 4 of 324
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP146569.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B1.H1.1"
                     /isolation_source="Hospital Wastewater"
                     /db_xref="taxon:562"
                     /plasmid="pB1.H1.1.c5"
                     /geo_loc_name="Poland:Olsztyn"
                     /lat_lon="53.77 N 20.48 E"
                     /collection_date="2021-04-20"
                     /collected_by="University of Warmia and Mazury in Olsztyn"
     gene            complement(607..894)
                     /locus_tag="V8956_RS00390"
                     /old_locus_tag="V8956_00390"
     CDS             complement(607..894)
                     /locus_tag="V8956_RS00390"
                     /old_locus_tag="V8956_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_338775140.1"
                     /translation="MRIKYESRWKLEELKLEICKSIDSLIDEYHIEYAMGINLYMNCED
                     KEFNPVDIVNEDGKNIMFVVSNNEKIQKPKKSAKKKIPAPVIELDKYRKK"
                     /name="hypothetical protein"
     gene            complement(907..1440)
                     /locus_tag="V8956_RS00395"
                     /old_locus_tag="V8956_00395"
     CDS             complement(907..1440)
                     /locus_tag="V8956_RS00395"
                     /old_locus_tag="V8956_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005707163.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000893324.1"
                     /translation="MLEQQFEDFEQQKRRDHQAQDQSKSAKQKRDRLNPPDWSQVTKAG
                     WNMLDSISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLRTIQYWIGDLES
                     IGAIVKIPVGIYYAYALDPYQVWRGYNKSKKYAAFNAKTLTQKDGVIDRKLRLMLSRRE
                     EFEGSLKTSNHDLD"
                     /name="replication/maintenance protein RepL"
     gene            complement(2429..2716)
                     /locus_tag="V8956_RS00400"
                     /old_locus_tag="V8956_00400"
     CDS             complement(2429..2716)
                     /locus_tag="V8956_RS00400"
                     /old_locus_tag="V8956_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023909281.1"
                     /translation="MRIKYESRWKLEELKLEICRSIDSLIDEYHIEYAMGINLYMNCED
                     KEFNPVDIVNEDGKNIMFVVSNNEKIQKPKKSAKKKIPAPVIELDKYRKK"
                     /name="hypothetical protein"
     gene            complement(2729..3262)
                     /locus_tag="V8956_RS00405"
                     /old_locus_tag="V8956_00405"
     CDS             complement(2729..3262)
                     /locus_tag="V8956_RS00405"
                     /old_locus_tag="V8956_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005707163.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000893324.1"
                     /translation="MLEQQFEDFEQQKRRDHQAQDQSKSAKQKRDRLNPPDWSQVTKAG
                     WNMLDSISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLRTIQYWIGDLES
                     IGAIVKIPVGIYYAYALDPYQVWRGYNKSKKYAAFNAKTLTQKDGVIDRKLRLMLSRRE
                     EFEGSLKTSNHDLD"
                     /name="replication/maintenance protein RepL"
     Typing          105..356
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.885"
                     /coverage="97.0"
                     /evalue="1.85e-99"
     Typing          1927..2178
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.885"
                     /coverage="97.0"
                     /evalue="1.85e-99"
ORIGIN
        1 ccgcatcagt gcgtacgtgc tcaatgtggt ttccggttct cttccgcttc ctcgcccact
       61 cgctcactaa cgttcggtcg ctggggctgc ggcgagcggt gccacctctc gcatgttatc
      121 ggtgattccg ctgttgttct ttcggggatg agtcgccaga tagctcttca aacgctctca
      181 gaagctctca gacgcacgaa acgggtttta aatgcccatc agggcatgga aggcgcgaaa
      241 aacgcttaca gagggtttta ggaggtcagg cgcgccgata cccctaacca ggggatttca
      301 ggtcgccagg aaccgcgtca gccccctgca aagcccattt gcacggaata ggcagccacg
      361 cggttggtag gtggtcgtag accgtcgatg ttacagcgat gggggtctgg ggttctcccc
      421 agacggtttc cggttgcgct caaggcgctg ccggaaccgt ggcggctcgg tcgttctgga
      481 aaacggggag caaaaagacg caagggattt tgaggcggca aaagagcaat gcaggataga
      541 caatcaacta gaaattgcat gcaatgttct gtggattgtc cgcctgccgc tggctctgta
      601 tggccatcac tttttccgat atttatctaa ctcaattaca ggtgcaggaa tttttttctt
      661 cgcacttttt tttggttttt gtattttttc attgttgcta acaacaaaca taatattctt
      721 tccatcttca ttgacaatat caacgggatt aaactcctta tcttcacaat tcatatatag
      781 atttattccc attgcatatt cgatatgata ttcatcaatt aacgaatcta ttgatttaca
      841 tatttctaat tttagttctt ctaatttcca acgtgattca tatttaattc tcatagcgca
      901 tcatacctag tccaagtcat gattagatgt cttaagcgag ccttcaaact cttctctcct
      961 agagagcatg agccgcaact tgcgatcaat aacgccatct ttttgagtta atgtttttgc
     1021 gttaaacgct gcatattttt tagatttatt atatccgcgc catacctgat acggatctaa
     1081 cgcataagca taatagatac ctactggtat tttcacgatg gcaccaatac tttctaaatc
     1141 tccaatccaa tattggatcg tccttaaact aacaccgaaa taatcagcta agaatttttg
     1201 gtcacataca acagccccac acgatttatc tatattttga gcaaaaaaag aatatagcct
     1261 aaatgctctg ggaaccttct cagatataga gtccaacata ttccagcccg ccttagtaac
     1321 ctgcgaccag tcaggtggat tcaaccgatc tcgtttttgc ttagcacttt tggattgatc
     1381 ttgtgcctga tgatctcgcc gtttttgctg ttcgaaatct tcaaattgct gttccagcat
     1441 acttgcatcc ttacctgcaa cgttgtttcg caccttacga aatttatatc gtacattacg
     1501 aaacaacaca cgcaaacatg tttgcgtata cacatgcaaa taacgtttcg tatgttggtg
     1561 tcataatgct ttgattttta agagaatgtc gatttttgct ctaaaaatct atcaaagcac
     1621 ttttttgcta cacaagattt aattagttca tcgaacattt ggggtgtcgg ggcgaagccc
     1681 tgacccagtc acgtagcgat agcggagtgt atactggctt actcttctgc aagcctcgcc
     1741 agccgtgatt ttgcccgatt tcgcgctctc cacgttcaac agctcgatca tgtttttgca
     1801 gtccgttcac cgctaaaacg ccccgcatca gtgcgtacgt gctcaatgtg gtttccggtt
     1861 ctcttccgct tcctcgccca ctcgctcact aacgttcggt cgctggggct gcggcgagcg
     1921 gtgccacctc tcgcatgtta tcggtgattc cgctgttgtt ctttcgggga tgagtcgcca
     1981 gatagctctt caaacgctct cagaagctct cagacgcacg aaacgggttt taaatgccca
     2041 tcagggcatg gaaggcgcga aaaacgctta cagagggttt taggaggtca ggcgcgccga
     2101 tacccctaac caggggattt caggtcgcca ggaaccgcgt cagccccctg caaagcccat
     2161 ttgcacggaa taggcagcca cgcggttggt aggtggtcgt agaccgtcga tgttacagcg
     2221 atgggggtct ggggttctcc ccagacggtt tccggttgcg ctcaaggcgc tgccggaacc
     2281 gtggcggctc ggtcgttctg gaaaacgggg agcaaaaaga cgcaagggat tttgaggcgg
     2341 caaaagagca atgcaggata gacaatcaac tagaaattgc atgcaatgtt ctgtggattg
     2401 tccgcctgcc gctggctctg tatggccatc actttttccg atatttatct aactcaatta
     2461 caggtgcagg aatttttttc ttcgcacttt tttttggttt ttgtattttt tcattgttgc
     2521 taacaacaaa cataatattc tttccatctt cattgacaat atcaacggga ttaaactcct
     2581 tatcttcaca attcatatat agatttattc ccattgcata ttcgatatga tattcatcaa
     2641 ttaacgaatc tattgatcta catatttcta attttagttc ttctaatttc caacgtgatt
     2701 catatttaat tctcatagcg catcatacct agtccaagtc atgattagat gtcttaagcg
     2761 agccttcaaa ctcttctctc ctagagagca tgagccgcaa cttgcgatca ataacgccat
     2821 ctttttgagt taatgttttt gcgttaaacg ctgcatattt tttagattta ttatatccgc
     2881 gccatacctg atacggatct aacgcataag cataatagat acctactggt attttcacga
     2941 tggcaccaat actttctaaa tctccaatcc aatattggat cgtccttaaa ctaacaccga
     3001 aataatcagc taagaatttt tggtcacata caacagcccc acacgattta tctatatttt
     3061 gagcaaaaaa agaatatagc ctaaatgctc tgggaacctt ctcagatata gagtccaaca
     3121 tattccagcc cgccttagta acctgcgacc agtcaggtgg attcaaccga tctcgttttt
     3181 gcttagcact tttggattga tcttgtgcct gatgatctcg ccgtttttgc tgttcgaaat
     3241 cttcaaattg ctgttccagc atacttgcat ccttacctgc aacgttgttt cgcaccttac
     3301 gaaatttata tcgtacatta cgaaacaaca cacgcaaaca tgtttgcgta tacacatgca
     3361 aataacgttt cgtatgttgg tgtcataatg ctttgatttt taagagaatg tcgatttttg
     3421 ctctaaagat ctatcaaagc acttttttgc tacacaagat ttaattagtt catcgaacat
     3481 ttggggtgtc ggggcgaagc cctgacccag tcacgtagcg atagcggagt gtatactggc
     3541 ttactcttct gcaagcctcg ccagccgtga ttttgcccga tttcgcgctc tccacgttca
     3601 acagctcgat catgtttttg cagtccgttc a
//
