LOCUS       NZ_CP146568            10328 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain B1.H1.1 plasmid pB1.H1.1.c6, complete
            sequence.
ACCESSION   NZ_CP146568
VERSION     NZ_CP146568.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40199718
            Assembly: GCF_037081875.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10328)
  AUTHORS   Rolbiecki,D.
  TITLE     Genomic and metagenomic analysis reveals shared resistance genes and
            mobile genetic elements in E. coli and Klebsiella spp. isolated from
            hospital patients and hospital wastewater at intra- and inter-genus
            level
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10328)
  AUTHORS   Rolbiecki,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-FEB-2024) Department of Water Protection Engineering
            and Environmental Microbiology, Uniwersytet Warminsko-Mazurski w
            Olsztynie, Prawochenskiego 1, Olsztyn 10-720, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: GS De Novo Assembler v. Flye 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 342x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037081875.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 18:26:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,233
            CDSs (total)                      :: 5,114
            Genes (coding)                    :: 4,790
            CDSs (with protein)               :: 4,790
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 180 of 324
            Pseudo Genes (incomplete)         :: 157 of 324
            Pseudo Genes (internal stop)      :: 35 of 324
            Pseudo Genes (multiple problems)  :: 45 of 324
            Pseudo Genes (short protein)      :: 4 of 324
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP146568.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B1.H1.1"
                     /isolation_source="Hospital Wastewater"
                     /db_xref="taxon:562"
                     /plasmid="pB1.H1.1.c6"
                     /geo_loc_name="Poland:Olsztyn"
                     /lat_lon="53.77 N 20.48 E"
                     /collection_date="2021-04-20"
                     /collected_by="University of Warmia and Mazury in Olsztyn"
     gene            complement(join(10179..10328,1..384))
                     /locus_tag="V8956_RS00340"
                     /old_locus_tag="V8956_00340"
     CDS             complement(join(10179..10328,1..384))
                     /locus_tag="V8956_RS00340"
                     /old_locus_tag="V8956_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(387..1313)
                     /locus_tag="V8956_RS00345"
                     /old_locus_tag="V8956_00345"
     CDS             complement(387..1313)
                     /locus_tag="V8956_RS00345"
                     /old_locus_tag="V8956_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1490..1888
                     /locus_tag="V8956_RS00350"
                     /old_locus_tag="V8956_00350"
     CDS             1490..1888
                     /locus_tag="V8956_RS00350"
                     /old_locus_tag="V8956_00350"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(2092..3006)
                     /locus_tag="V8956_RS00355"
                     /old_locus_tag="V8956_00355"
     CDS             complement(2092..3006)
                     /locus_tag="V8956_RS00355"
                     /old_locus_tag="V8956_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271363.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            4740..4949
                     /locus_tag="V8956_RS00360"
                     /old_locus_tag="V8956_00360"
     CDS             4740..4949
                     /locus_tag="V8956_RS00360"
                     /old_locus_tag="V8956_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(5015..5548)
                     /locus_tag="V8956_RS00365"
                     /old_locus_tag="V8956_00365"
     CDS             complement(5015..5548)
                     /locus_tag="V8956_RS00365"
                     /old_locus_tag="V8956_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(5551..6477)
                     /locus_tag="V8956_RS00370"
                     /old_locus_tag="V8956_00370"
     CDS             complement(5551..6477)
                     /locus_tag="V8956_RS00370"
                     /old_locus_tag="V8956_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            6654..7052
                     /locus_tag="V8956_RS00375"
                     /old_locus_tag="V8956_00375"
     CDS             6654..7052
                     /locus_tag="V8956_RS00375"
                     /old_locus_tag="V8956_00375"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(7256..8170)
                     /locus_tag="V8956_RS00380"
                     /old_locus_tag="V8956_00380"
     CDS             complement(7256..8170)
                     /locus_tag="V8956_RS00380"
                     /old_locus_tag="V8956_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271363.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            9904..10113
                     /locus_tag="V8956_RS00385"
                     /old_locus_tag="V8956_00385"
     CDS             9904..10113
                     /locus_tag="V8956_RS00385"
                     /old_locus_tag="V8956_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     Typing          complement(2093..3006)
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.909"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7257..8170)
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.909"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(391..1313)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5555..6477)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agccactgac accccaccca gccatttcca gctataccct ttaagggtgt tgtattcctg
       61 cacgatattg ctgtgaattg tcgcaatatc cctgttcagg gcatccgcgt ttgccttcat
      121 gtttgcgact gactcttcca gccgctgaaa atcctggctg atgcgctcac ctatcagtcc
      181 tgccacgtcc agcttctgca cctcatttct gacttcatcc ctgacctgtt ttgccacacc
      241 gtcagaaata tcccgatatc cgcgaagagt ttcccttgcc acagccagag aatcagcaac
      301 agctttctgt tgctccattg cggtatgttg cagatgctcc accgataaca ggagcgtgag
      361 taatttttct tctgtgctgg ccatgttcag aatcccatag aaaatccacg tgacggtgat
      421 ttaacccgtt caggtgcagt tcggttctgt ctgctcactc tgtctctcat gtcaaagcca
      481 tgctcccgcg caacatcatg ttgcagcctt ctgaccagtt catgttgctt aaccggagtg
      541 tccagccatt ccctgatgtc atgcccgacc gttcgcgcct gttcaagatg gcgggaatag
      601 tccggttcag accgtttttg catgatgtca tgacgctggg catcatcaag ccgtccggca
      661 gcttcaaaac ccagatgcac ttcgggacgt cgttcaatgc cctgttcttt cagcgtccgg
      721 cagtcaatga agcccctttc aaagccatgc tttctcatat gttcgttgtg cttcacctcc
      781 cagcgttttc gcaattcccg caactgctct ttttgctgag tgagagtcag gcttccggaa
      841 tcctttttcg cacctccccg ctcgggatac ctggcgttat agcgccggaa atactgttcc
      901 ggtgtacgtt caatgccatc attcacacgc tgactcatca tgatatgcgc atgaggctgt
      961 tcgccgccgt caatgctcgc tttcggattg tgaatggcaa aactccaggc gtgccgttcg
     1021 ccagcttcct gacgtacaaa atcctgcacc agttcaaggc gctgttccgg tgtcagttca
     1081 cggggtaatg caatttcaag ttccctatag gtcgaaccgt tcgcacgttc aaactggtca
     1141 gccgcctgcc agaaatggga aggattatcc ctggcccatt ccggcatgtt tccgtgtgcg
     1201 gaatattcca aatcctgacg gtcgcggtat ttctctttgc gctcgatgta atcggcgtgg
     1261 tttgcagcct gccccttgcc accaaattta acgctgaggt gataggttgc cacggtttca
     1321 gccctctgca ggagcgcact gtttctcgac gaaggagaga aacatataag tgcgcttcct
     1381 tctaatttta cgaattagca cggaagatac cccctgcacc cgccgaccgc aagcgatata
     1441 ctgcacgacc acaacacacc gcaaaaaaac accagggagc atcacgaaca tgcgcatcag
     1501 caacatcgaa tggctgaaga aacggatcgg attcatcagg aagctcggag aacagaccgc
     1561 acgacagaga cagattattg acctgatcga caacgaagcc ggactcacag aacaggaacg
     1621 caaacttctc cacgtactgg caacagcgga aaaaaacgac ttacaggcac aggagagcga
     1681 acgaaaacag gcggtccaaa aacgaatcga gggaaaaaaa caacgacggg aacgcaatca
     1741 ccggctcttt ctcgcggctg ggctactgat cgaagcagga ctggtagaca caaaaacagg
     1801 cgagctatgc tataaaaaag acaggatatt acaagcactt aaggaactta agtacgatct
     1861 ggagacatcc ccaaacccag acgcgtaagt gcctccatcc gtgttccgat tccctggtgc
     1921 ttcctcgctc attcgctcgc tacgctcggt catgagactg cggcgagcac taccaccaca
     1981 atcacgcgga aagccgcgcc attgcagggc tgaacgggga aaaacggcaa aaaacctgct
     2041 gctcaaaaag acgccagaaa ggcgctgtta tctgatatag gcttatttgg tctactctac
     2101 gagacctcgt tttttcagtt ggtaatacca ggcgcgactg atccctaatg cctcccacgg
     2161 tttcagcgtg cgtgcagatg tagctactgt gctgcgctta gacttagaac cgcccttgcg
     2221 accgcgtgca gcctgaattt ctggcgtgtg cgtatcagcc acatactgtg cgaaagtttc
     2281 cggcgtgaag ttcctgtgcg tgtatttcgc aatgctcttg ccaatagccc gacattcagc
     2341 cagtgaaagc ggaacgggaa gcgatgcatt gtacatttcg acacgctgga tcaccgcttc
     2401 aagccactgt gagaacgctg gccagccctg acgaatcgcc ctgtaagccc atttgcgcgt
     2461 catttcgaac aggtggcagt ttcgccccat cccgtaatgt ttatcgatgc tacgacgctc
     2521 tgaggcgctc aaatcgagat aatcagccag ttcatcgagg gtataggctt cctcgcgcca
     2581 ctccatcacc agccattcca ggtggaacgg atttttgcaa atcaggccgc tgtagttcac
     2641 atccgcacaa agtttctcac acagcgaacg ttcaaccgct gcggcatatt tcagcgcctt
     2701 aaccgaggca tcaggcgcgg ttctcacggc gatgttgagt gcgtagagca ggtgagcgtg
     2761 tccgttcaca ggatttttaa cggtgatgtt cggcgcggga gcgttccggt cgctccagtc
     2821 aatcgcagcc ccctcgcggt caacgtcaaa aaccagccag aactgtgcat gaggctggtt
     2881 ctgctggata taccgtgcaa gaagcgcgcg ccctttgccg gagatacgaa ggccgaaagc
     2941 gagatcatcc gtgtgatagg gacggtgggg taaattttct tcgaagtatt gaagcgcggc
     3001 gctcatgtgt tacgattcct tccaggttat cgaccgacat cgcccctgta ttttcgtgaa
     3061 agtcttggcg gatagacacg aatttcaggg gcgtttgttt ttcagtcgct tacactaccc
     3121 ccaacgacaa aacctgtcga gccttcccgc aaatccgatc catttaccat aactcccgtt
     3181 gtacgtaccc cttaaaacgc cctcagtcgc tttctggcgc aaaaaacgca aaaagacaag
     3241 aaaacaccag gccaaaccag aaaaacgcgt cagaatcgtt ttcagggcgt tgcataacta
     3301 tgcacgaaag cgatgtgcga tcggtttcgt gcgttctgaa caccgaacgg ctcaaaacga
     3361 caagttttct cccctggtgc gtggcgctct gacggggttt cctgtttcca aacttcacat
     3421 ctcgccagga caaaaaaaac cgacctgggg tcggtggcaa gaatcagagt ctatgggaaa
     3481 tattttagtt atcggatgct caatattcac aaacgcaaaa aatgcctcag tgatgcccta
     3541 aaacaacctt tccagaagga gtccgcgctt tacgatgcac ggaagagata aaaaacccca
     3601 caaaagtggg gaaccaagag aaacaacaat tacaggttgt tggttgtgtt cgaacaacga
     3661 tgaagtagct acgctaaata ataattaccg tacaggccct acatattcaa cactaaattt
     3721 aagatcctga cgaaggattt ataaatgcac gaaaacacat agacagacaa attcactaaa
     3781 ttcatacaca aaaacaagtg tagttttcta agtaaattag attcgacaac caattagttt
     3841 atccacatcg cattccactc ttgctacacc aatcacattt cttgatatgt aatagcagaa
     3901 aaaataaaac cagacaccaa tagaccaaaa aacaaacatt caacaagaaa cattttgaac
     3961 aatatcacaa aactgtcaca accatcatca gactattgaa aaaaactggt tgctgtttta
     4021 taaaagcgac tgtaacaacc tccactctat agttccatta gtttaaagtg tcacacaaac
     4081 tgagcatcca tttgttttcc ttacttttgc ccctgtattc caaggggttt ttttataact
     4141 aaaagattag cacaccacac aaaaacaata aaaaaaaacg acaagttata caataaaacc
     4201 agtactatcc atacagctaa ttacatataa atactattaa accatctgga cattaaaaat
     4261 aaactcttat aacataaaat taatgcggtt ccggtaccat atgtcaggga taatgtgcaa
     4321 atggaggtat cggaactgca tttcagttca ttattaatta atgacaaaaa aaacctctgc
     4381 gagcgcagag ggggtaagga atgttcccgg tcataatatg tcgatagtgt ttatatacat
     4441 acagaacaag aacaacaacc agccaactaa taaatatttt attttttaga gctgaaacct
     4501 taaagctcac ttccattcca tacattgtta taaaccttat gttgaaaact gaaagtaagt
     4561 acggcagact tattaagttg cgtgcagtct tttcacgtta tacaaacaag aacacctgcg
     4621 aagttatttc tccttacata tccccctatg ccaacggggg attttttctg gttcactaac
     4681 gcataaataa tagtgacacc ctatcattca caaaccgtga taatataagg attatcatca
     4741 tggcaaaatt aggtgaaaac accccacata ttatcgatgc tactgtagat tttatggcat
     4801 caagccaggc atttcgtgag tatctgaaaa aacttccccc tgataacaca ataccgccag
     4861 ctcttactga tgagcacgta ccgttatata tacgaaggct ggaatactat cgtctgcttt
     4921 atcgaccaaa gcagacagaa tcatattgaa aaaaacggtg aacggttcgc gaaatccttt
     4981 cgcgggtcgt gaggcgttgg acaagcgaag cgcgtcagtt tttccggctc ttccccgtga
     5041 caggtttctc caccagttta tcttccagtg tcacaacttt ctgatacacc gccgctgtgg
     5101 cgttgtagtt cctgtcgagt attgttttca ttccccatcc gagcacacag aacataacca
     5161 gaaaagccac tgacacccca cccagccatt tccagctata ccctttaagg gtgttgtatt
     5221 cctgcacgat attgctgtga attgtcgcaa tatccctgtt cagggcatcc gcgtttgcct
     5281 tcatgtttgc gactgactct tccagccgct gaaaatcctg gctgatgcgc tcacctatca
     5341 gtcctgccac gtccagcttc tgcacctcat ttctgacttc atccctgacc tgttttgcca
     5401 caccgtcaga aatatcccga tatccgcgaa gagtttccct tgccacagcc agagaatcag
     5461 caacagcttt ctgttgctcc attgcggtat gttgcagatg ctccaccgat aacaggagcg
     5521 tgagtaattt ttcttctgtg ctggccatgt tcagaatccc atagaaaatc cacgtgacgg
     5581 tgatttaacc cgttcaggtg cagttcggtt ctgtctgctc actctgtctc tcatgtcaaa
     5641 gccatgctcc cgcgcaacat catgttgcag ccttctgacc agttcatgtt gcttaaccgg
     5701 agtgtccagc cattccctga tgtcatgccc gaccgttcgc gcctgttcaa gatggcggga
     5761 atagtccggt tcagaccgtt tttgcatgat gtcatgacgc tgggcatcat caagccgtcc
     5821 ggcagcttca aaacccagat gcacttcggg acgtcgttca atgccctgtt ctttcagcgt
     5881 ccggcagtca atgaagcccc tttcaaagcc atgctttctc atatgttcgt tgtgcttcac
     5941 ctcccagcgt tttcgcaatt cccgcaactg ctctttttgc tgagtgagag tcaggcttcc
     6001 ggaatccttt ttcgcacctc cccgctcggg atacctggcg ttatagcgcc ggaaatactg
     6061 ttccggtgta cgttcaatgc catcattcac acgctgactc atcatgatat gcgcatgagg
     6121 ctgttcgccg ccgtcaatgc tcgctttcgg attgtgaatg gcaaaactcc aggcgtgccg
     6181 ttcgccagct tcctgacgta caaaatcctg caccagttca aggcgctgtt ccggtgtcag
     6241 ttcacggggt aatgcaattt caagttccct ataggtcgaa ccgttcgcac gttcaaactg
     6301 gtcagccgcc tgccagaaat gggaaggatt atccctggcc cattccggca tgtttccgtg
     6361 tgcggaatat tccaaatcct gacggtcgcg gtatttctct ttgcgctcga tgtaatcggc
     6421 gtggtttgca gcctgcccct tgccaccaaa tttaacgctg aggtgatagg ttgccacggt
     6481 ttcagccctc tgcaggagcg cactgtttct cgacgaagga gagaaacata taagtgcgct
     6541 tccttctaat tttacgaatt agcacggaag ataccccctg cacccgccga ccgcaagcga
     6601 tatactgcac gaccacaaca caccgcaaaa aaacaccagg gagcatcacg aacatgcgca
     6661 tcagcaacat cgaatggctg aagaaacgga tcggattcat caggaagctc ggagaacaga
     6721 ccgcacgaca gagacagatt attgacctga tcgacaacga agccggactc acagaacagg
     6781 aacgcaaact tctccacgta ctggcaacag cggaaaaaaa cgacttacag gcacaggaga
     6841 gcgaacgaaa acaggcggtc caaaaacgaa tcgagggaaa aaaacaacga cgggaacgca
     6901 atcaccggct ctttctcgcg gctgggctac tgatcgaagc aggactggta gacacaaaaa
     6961 caggcgagct atgctataaa aaagacagga tattacaagc acttaaggaa cttaagtacg
     7021 atctggagac atccccaaac ccagacgcgt aagtgcctcc atccgtgttc cgattccctg
     7081 gtgcttcctc gctcattcgc tcgctacgct cggtcatgag actgcggcga gcactaccac
     7141 cacaatcacg cggaaagccg cgccattgca gggctgaacg gggaaaaacg gcaaaaaacc
     7201 tgctgctcaa aaagacgcca gaaaggcgct gttatctgat ataggcttat ttggtctact
     7261 ctacgagacc tcgttttttc agttggtaat accaggcgcg actgatccct aatgcctccc
     7321 acggtttcag cgtgcgtgca gatgtagcta ctgtgctgcg cttagactta gaaccgccct
     7381 tgcgaccgcg tgcagcctga atttctggcg tgtgcgtatc agccacatac tgtgcgaaag
     7441 tttccggcgt gaagttcctg tgcgtgtatt tcgcaatgct cttgccaata gcccgacatt
     7501 cagccagtga aagcggaacg ggaagcgatg cattgtacat ttcgacacgc tggatcaccg
     7561 cttcaagcca ctgtgagaac gctggccagc cctgacgaat cgccctgtaa gcccatttgc
     7621 gcgtcatttc gaacaggtgg cagtttcgcc ccatcccgta atgtttatcg atgctacgac
     7681 gctctgaggc gctcaaatcg agataatcag ccagttcatc gagggtatag gcttcctcgc
     7741 gccactccat caccagccat tccaggtgga acggattttt gcaaatcagg ccgctgtagt
     7801 tcacatccgc acaaagtttc tcacacagcg aacgttcaac cgctgcggca tatttcagcg
     7861 ccttaaccga ggcatcaggc gcggttctca cggcgatgtt gagtgcgtag agcaggtgag
     7921 cgtgtccgtt cacaggattt ttaacggtga tgttcggcgc gggagcgttc cggtcgctcc
     7981 agtcaatcgc agccccctcg cggtcaacgt caaaaaccag ccagaactgt gcatgaggct
     8041 ggttctgctg gatataccgt gcaagaagcg cgcgcccttt gccggagata cgaaggccga
     8101 aagcgagatc atccgtgtga tagggacggt ggggtaaatt ttcttcgaag tattgaagcg
     8161 cggcgctcat gtgttacgat tccttccagg ttatcgaccg acatcgcccc tgtattttcg
     8221 tgaaagtctt ggcggataga cacgaatttc aggggcgttt gtttttcagt cgcttacact
     8281 acccccaacg acaaaacctg tcgagccttc ccgcaaatcc gatccattta ccataactcc
     8341 cgttgtacgt accccttaaa acgccctcag tcgctttctg gcgcaaaaaa cgcaaaaaga
     8401 caagaaaaca ccaggccaaa ccagaaaaac gcgtcagaat cgttttcagg gcgttgcata
     8461 actatgcacg aaagcgatgt gcgatcggtt tcgtgcgttc tgaacaccga acggctcaaa
     8521 acgacaagtt ttctcccctg gtgcgtggcg ctctgacggg gtttcctgtt tccaaacttc
     8581 acatctcgcc aggacaaaaa aaaccgacct ggggtcggtg gcaagaatca gagtctatgg
     8641 gaaatatttt agttatcgga tgctcaatat tcacaaacgc aaaaaatgcc tcagtgatgc
     8701 cctaaaacaa cctttccaga aggagtccgc gctttacgat gcacggaaga gataaaaaac
     8761 cccacaaaag tggggaacca agagaaacaa caattacagg ttgttggttg tgttcgaaca
     8821 acgatgaagt agctacgcta aataataatt accgtacagg ccctacatat tcaacactaa
     8881 atttaagatc ctgacgaagg atttataaat gcacgaaaac acatagacag acaaattcac
     8941 taaattcata cacaaaaaca agtgtagttt tctaagtaaa ttagattcga caaccaatta
     9001 gtttatccac atcgcattcc actcttgcta caccaatcac atttcttgat atgtaatagc
     9061 agaaaaaata aaaccagaca ccaatagacc aaaaaacaaa cattcaacaa gaaacatttt
     9121 gaacaatatc acaaaactgt cacaaccatc atcagactat tgaaaaaaac tggttgctgt
     9181 tttataaaag cgactgtaac aacctccact ctatagttcc attagtttaa agtgtcacac
     9241 aaactgagca tccatttgtt ttccttactt ttgcccctgt attccaaggg gtttttttat
     9301 aactaaaaga ttagcacacc acacaaaaac aataaaaaaa aacgacaagt tatacaataa
     9361 aaccagtact atccatacag ctaattacat ataaatacta ttaaaccatc tggacattaa
     9421 aaataaactc ttataacata aaattaatgc ggttccggta ccatatgtca gggataatgt
     9481 gcaaatggag gtatcggaac tgcatttcag ttcattatta attaatgaca aaaaaaacct
     9541 ctgcgagcgc agagggggta aggaatgttc ccggtcataa tatgtcgata gtgtttatat
     9601 acatacagaa caagaacaac aaccagccaa ctaataaata ttttattttt tagagctgaa
     9661 accttaaagc tcacttccat tccatacatt gttataaacc ttatgttgaa aactgaaagt
     9721 aagtacggca gacttattaa gttgcgtgca gtcttttcac gttatacaaa caagaacacc
     9781 tgcgaagtta tttctcctta catatccccc tatgccaacg ggggattttt tctggttcac
     9841 taacgcataa ataatagtga caccctatca ttcacaaacc gtgataatat aaggattatc
     9901 atcatggcaa aattaggtga aaacacccca catattatcg atgctactgt agattttatg
     9961 gcatcaagcc aggcatttcg tgagtatctg aaaaaacttc cccctgataa cacaataccg
    10021 ccagctctta ctgatgagca cgtaccgtta tatatacgaa ggctggaata ctatcgtctg
    10081 ctttatcgac caaagcagac agaatcatat tgaaaaaaac ggtgaacggt tcgcgaaatc
    10141 ctttcgcggg tcgtgaggcg ttggacaagc gaagcgcgtc agtttttccg gctcttcccc
    10201 gtgacaggtt tctccaccag tttatcttcc agtgtcacaa ctttctgata caccgccgct
    10261 gtggcgttgt agttcctgtc gagtattgtt ttcattcccc atccgagcac acagaacata
    10321 accagaaa
//
