LOCUS       NZ_CP146566             4091 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain B1.H1.1 plasmid pB1.H1.1.c8, complete
            sequence.
ACCESSION   NZ_CP146566
VERSION     NZ_CP146566.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40199718
            Assembly: GCF_037081875.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Rolbiecki,D.
  TITLE     Genomic and metagenomic analysis reveals shared resistance genes and
            mobile genetic elements in E. coli and Klebsiella spp. isolated from
            hospital patients and hospital wastewater at intra- and inter-genus
            level
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Rolbiecki,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-FEB-2024) Department of Water Protection Engineering
            and Environmental Microbiology, Uniwersytet Warminsko-Mazurski w
            Olsztynie, Prawochenskiego 1, Olsztyn 10-720, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: GS De Novo Assembler v. Flye 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 342x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037081875.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 18:26:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,233
            CDSs (total)                      :: 5,114
            Genes (coding)                    :: 4,790
            CDSs (with protein)               :: 4,790
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 180 of 324
            Pseudo Genes (incomplete)         :: 157 of 324
            Pseudo Genes (internal stop)      :: 35 of 324
            Pseudo Genes (multiple problems)  :: 45 of 324
            Pseudo Genes (short protein)      :: 4 of 324
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP146566.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B1.H1.1"
                     /isolation_source="Hospital Wastewater"
                     /db_xref="taxon:562"
                     /plasmid="pB1.H1.1.c8"
                     /geo_loc_name="Poland:Olsztyn"
                     /lat_lon="53.77 N 20.48 E"
                     /collection_date="2021-04-20"
                     /collected_by="University of Warmia and Mazury in Olsztyn"
     gene            join(3618..4091,1..1674)
                     /gene="mobQ"
                     /locus_tag="V8956_RS00005"
                     /old_locus_tag="V8956_00005"
     CDS             join(3618..4091,1..1674)
                     /gene="mobQ"
                     /locus_tag="V8956_RS00005"
                     /old_locus_tag="V8956_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_011666798.1"
                     /translation="MAIYHLSMKILSRSKGYSAVASAAYRAGEKILDERTGVIHDYTRK
                     NGVASAVILTPANAPAWCANRAELWNAVEKAERRKNSQLAREFELAIPRELAQDAARET
                     VLNFVRENFVSRGMIADVAFHNLGGSNPHAHIMLSMRAITPDGFGEKVREWNDWTHAET
                     WRGSWADHANRALANAGYQEEIDHRSYERQGVEKTPGIHLGKSACAMEKWGIETERGEQ
                     NRLINRLNLEIQVSRDELRNSELTEPARRMADLLNIELPDNATSYILHDIIEALPTDSN
                     AAWKLTSKAMSMMADMQATRRRWEDLNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWV
                     APEYGREQEKIQSLSQRMEKLRQHHWKVEKQDIPASQKAFETQWQQWGASGLADLREQL
                     RKREEELRRKEQEETERRRAEQLRRNDNAVMDSGVLKAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRARIFESVDLMRDRSGYICERSEIGQRPPLQHVYSSATFEQ
                     LLTQVCQTWPQHTRDLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSMGGVVRLNGTLYPAFAVPTADNSQLVINALTDKGLRFA
                     GYGEAVNHDADSPARPAPELMQFHLKTWSEPLFAIVNTPEKQPDHLFRSLGFERTWDEW
                     KRDEYARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(1923..2624)
                     /locus_tag="V8956_RS00010"
                     /old_locus_tag="V8956_00010"
     CDS             complement(1923..2624)
                     /locus_tag="V8956_RS00010"
                     /old_locus_tag="V8956_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_011666799.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGSDAESTEL
                     QKTMTVTAEEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCVDELNQKSDLAVTLETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3157..3447)
                     /locus_tag="V8956_RS00015"
                     /old_locus_tag="V8956_00015"
     CDS             complement(3157..3447)
                     /locus_tag="V8956_RS00015"
                     /old_locus_tag="V8956_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001514113.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     GETQLRAWLLHLLKTEVTRQADVSRIQPLIDELNALPKPVPKEVSENDQQA"
                     /name="hypothetical protein"
     Typing          complement(3452..3602)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.81"
                     /coverage="85.0"
                     /evalue="3.53e-55"
     Typing          complement(3619..3973)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1925..2624)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.158"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2..1671
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 actcacgccg aaacatggcg gggatcatgg gcagaccatg cgaacagggc gctggcgaac
       61 gccggttatc aggaagagat agaccatcgt tcatacgagc gacagggggt tgagaaaacg
      121 cctggcattc acctcggaaa gtcggcctgt gcgatggaaa agtggggcat cgaaacagaa
      181 cgcggcgaac agaatcgcct gattaacagg cttaacctgg aaatacaggt ttcccgcgac
      241 gaactcagga acagtgagct gacagagccc gcacgccgta tggctgacct tctgaatatt
      301 gagcttcctg acaacgcaac ttcgtacata ctgcatgaca tcatcgaagc gttgccgacg
      361 gacagtaacg cagcatggaa actcaccagt aaagccatgt cgatgatggc agatatgcag
      421 gcgacgcgca gacgctggga agatctgaac accgaacgaa aggaagcgat gagccatgct
      481 gccagcctga aaaaatcatc gccgttttcg tccggttttt cccgtattcc cctgatgcaa
      541 tgggttgcac cggaatacgg ccgcgaacag gaaaaaatcc agtcgttgag ccagcgcatg
      601 gaaaaattgc ggcagcatca ctggaaggtt gagaaacagg acattcccgc gagtcaaaaa
      661 gcatttgaaa cacagtggca gcagtggggt gcatctggcc ttgcagatct ccgagaacag
      721 ttgcgtaaac gtgaagagga attgcgccgg aaggagcagg aggaaacaga acgtcgtcgg
      781 gcggagcagc tcaggagaaa tgacaatgcc gttatggatt caggggtact gaaagcagtc
      841 gttacggggt atggcgaggc ccccatgcca ggtaacgggg aaatgacctg ttaccttctg
      901 cttcacaacc ggaacggaga atatacgctc tgggggaacg aactggagaa ataccgcgcc
      961 agaatttttg agagtgttga tttaatgcgt gacaggagtg gttatatctg tgagcgaagc
     1021 gaaatcggtc aacgtccacc actgcaacat gtttattcct ctgcgacctt tgagcaactg
     1081 ctgacgcagg tttgccagac atggccgcaa catacccgcg atctccgcca gccgaagaca
     1141 tggccggagt ctttctgtct tggagaagac cgccagccag ccatgccatc actggcggca
     1201 aggaaggtgg attttacgca gggacgtctg ccaccgactc tgatgccggt aatgagttct
     1261 gtcgaccgtg aaacccgcca gcttcagctt ttgctggtca tgggtgtgga tgactctatg
     1321 ggcggtgttg tccggcttaa tggtacgctg tatcccgcgt ttgccgtccc cacagccgac
     1381 aacagccagc tggtaatcaa tgcactgacc gacaaaggtt tacgctttgc aggctatggt
     1441 gaagcggtaa accacgatgc tgacagtccg gcacgtcctg cgccggaact tatgcagttt
     1501 catctgaaaa cctggagcga accgcttttt gctatcgtca acacgccaga aaaacagccg
     1561 gatcatctgt tccgctcgct gggctttgag cgcacctggg atgaatggaa acgtgatgaa
     1621 tatgcccgaa cacacgccac cgagaggaga catgatcgcg gttggtcaca gtgaatgtga
     1681 ccaatatttt gttttgttta acatcgtgtt atgttcgtcg atgttcatcg acagggatga
     1741 atgcaagaag cacacacata acacaaatca actttatagt tttcccgccc ccgaaaaatt
     1801 gggggttctt ttttcactgt gccagagcgt ttatgacgca attttttact tcaccagtac
     1861 cttcgcatgc caaacacaaa aaaaacgcgt cagagagcgt tttagaggcc ttaaaatcga
     1921 ttctagatcg tcatcttgat ctgtttgtct tccttgaagc gaaaatgcag ggctacaacc
     1981 gtgcgcccct tcttgatcgt ctcaagcgtt acagccaagt cgcttttttg attcagttcg
     2041 tcaacgcaag gtcttaataa ttgctggttc agagacttat atgtttgata tttatgctct
     2101 atatcaagca ttgagcgaaa atcatcgagg gcgataatac gttcccctgt gctgcggaat
     2161 tgctggagaa gttcgtatat tcttatgctg taacttcttg ataaattgga gatgttctta
     2221 attacaactc tagtaaattg ccccttcaac tgtgtcagat aaggcatgac cgcatctgaa
     2281 aaagtgattt gggctttgcc ctcaccttta gcgtactgtg cccgatactg gatccagcgg
     2341 aactcctcgc gcttctcatc gtccttaagg atgatcgacc tgtcccacaa acgatcaatt
     2401 gcttcctgta gctgaacctc ggcatttttc cgccccatat cagggaatga gtcgaaatac
     2461 tcttcggcgg taacagtcat ggtcttttgc aattcagtgg attctgcatc agaacccgac
     2521 ttcaatcgac caatgcaaag taacaagaat cgctgttcct gaagagtcaa tttatagctg
     2581 gcctcaacca gtgcattgga tttgtaggca atcaaatccg acattgtaaa tccctcaacc
     2641 taaggtaatg agtaaaatat aaacattcct ttaggcaaag gcaatatgat ttaattttaa
     2701 tatccctttt cctgtgaata ttaccttaaa tcatattgcc tttaccttaa atcatattgc
     2761 ctatccacct taaatcatat tgcctttacc ttaaattata ttacctatag tcttctaaac
     2821 tcttgaattt cataagaaaa ttcgacccta aaacaagtat taaaaacaaa atcttaaaaa
     2881 caaaatctaa aaacttaacg gcgagcctgt ggaaaacttg gggtgttcaa aacaaaaaat
     2941 catgaaaaac aacaggttac aaaaaagcag gttcgcaagc tttagcaaaa ccccgctcaa
     3001 ccctgtggaa atccagtatt catgcgggtt cagcgcatat cacctgtcga aaaaccgtgc
     3061 gtatgtgctc aatctggcga cggattcact tccgcttcct cgcccactcg ctcgctaacg
     3121 ctcggtcgct ggggctgcgg cgagcggacg gtgcattcac gcctgttggt cgttttctga
     3181 cacttctttg gggactggtt ttggtaacgc gttcagttcg tcaatcaacg gctgtatcct
     3241 gctcacatca gcctgccgtg ttacctctgt cttcaggaga tgaagcaacc aggctcgtag
     3301 ttgtgtttcc ccaggcttcc tgacctttgc cagcaatgcc gctcccacaa tgaccttctg
     3361 cgctgtgtcc aggctcctgc tcttcgcctt cagacgctgc aatctggcct cagcatcggc
     3421 aatcttctgt tcgagtgttc tactcattcc gtaactccgt gcgcggtgaa aaatcgcatt
     3481 ttagcgcgtc actggtagtt taaaaactga actggcataa tgcacagcaa atcacgaagt
     3541 gcgcacttat acaaactctc cttcgtcgag tttgtgtgcg ccctgcgggg ctaaaagaaa
     3601 acggcaacaa cgacacgatg gcaatttatc atctcagcat gaagattctt tccagaagca
     3661 agggctacag cgccgttgct tctgctgcct atcgtgccgg agaaaaaata ctggatgagc
     3721 gcaccggagt gattcacgac tacacccgca aaaacggtgt agccagcgca gtgattctga
     3781 cgccagcaaa cgccccagca tggtgtgcga accgcgccga actctggaat gctgttgaaa
     3841 aagccgagcg gcggaaaaac tcacaacttg cgcgggagtt cgaactcgcc atcccccgtg
     3901 aactggcgca ggatgccgca cgggaaacag ttctcaactt cgtaagggaa aactttgtca
     3961 gtcgcggcat gatcgccgac gtggcctttc acaatctggg cgggtcaaac ccacacgcgc
     4021 acatcatgct atccatgagg gcaatcacgc ctgacggctt cggggagaaa gtccgtgagt
     4081 ggaatgactg g
//
