LOCUS       NZ_CP146563             8182 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain B1.H1.18 plasmid pB1.H1.18.c3, complete
            sequence.
ACCESSION   NZ_CP146563
VERSION     NZ_CP146563.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40199719
            Assembly: GCF_037081895.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Rolbiecki,D.
  TITLE     Genomic and metagenomic analysis reveals shared resistance genes and
            mobile genetic elements in E. coli and Klebsiella spp. isolated from
            hospital patients and hospital wastewater at intra- and inter-genus
            level
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8182)
  AUTHORS   Rolbiecki,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-FEB-2024) Department of Water Protection Engineering
            and Environmental Microbiology, Uniwersytet Warminsko-Mazurski w
            Olsztynie, Prawochenskiego 1, Olsztyn 10-720, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: GS De Novo Assembler v. Flye 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 392x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037081895.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 17:58:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,239
            CDSs (total)                      :: 5,120
            Genes (coding)                    :: 4,773
            CDSs (with protein)               :: 4,773
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 347
            CDSs (without protein)            :: 347
            Pseudo Genes (ambiguous residues) :: 0 of 347
            Pseudo Genes (frameshifted)       :: 199 of 347
            Pseudo Genes (incomplete)         :: 160 of 347
            Pseudo Genes (internal stop)      :: 35 of 347
            Pseudo Genes (multiple problems)  :: 44 of 347
            Pseudo Genes (short protein)      :: 4 of 347
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP146563.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B1.H1.18"
                     /isolation_source="Hospital Wastewater"
                     /db_xref="taxon:562"
                     /plasmid="pB1.H1.18.c3"
                     /geo_loc_name="Poland:Olsztyn"
                     /lat_lon="53.77 N 20.48 E"
                     /collection_date="2021-04-20"
                     /collected_by="University of Warmia and Mazury in Olsztyn"
     gene            join(7873..8182,1..1838)
                     /gene="mobQ"
                     /locus_tag="V8955_RS00475"
                     /old_locus_tag="V8955_00475"
     CDS             join(7873..8182,1..1838)
                     /gene="mobQ"
                     /locus_tag="V8955_RS00475"
                     /old_locus_tag="V8955_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_011666798.1"
                     /translation="MAIYHLSMKILSRSKGYSAVASAAYRAGEKILDERTGVIHDYTRK
                     NGVASAVILTPANAPAWCANRAELWNAVEKAERRKNSQLAREFELAIPRELAQDAARET
                     VLNFVRENFVSRGMIADVAFHNLGGSNPHAHIMLSMRAITPDGFGEKVREWNDWTHAET
                     WRGSWADHANRALANAGYQEEIDHRSYERQGVEKTPGIHLGKSACAMEKWGIETERGEQ
                     NRLINRLNLEIQVSRDELRNSELTEPARRMADLLNIELPDNATSYILHDIIEALPTDSN
                     AAWKLTSKAMSMMADMQATRRRWEDLNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWV
                     APEYGREQEKIQSLSQRMEKLRQHHWKVEKQDIPASQKAFETQWQQWGASGLADLREQL
                     RKREEELRRKEQEETERRRAEQLRRNDNAVMDSGVLKAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRARIFESVDLMRDRSGYICERSEIGQRPPLQHVYSSATFEQ
                     LLTQVCQTWPQHTRDLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSMGGVVRLNGTLYPAFAVPTADNSQLVINALTDKGLRFA
                     GYGEAVNHDADSPARPAPELMQFHLKTWSEPLFAIVNTPEKQPDHLFRSLGFERTWDEW
                     KRDEYARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(2087..2788)
                     /locus_tag="V8955_RS00480"
                     /old_locus_tag="V8955_00480"
     CDS             complement(2087..2788)
                     /locus_tag="V8955_RS00480"
                     /old_locus_tag="V8955_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_011666799.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGSDAESTEL
                     QKTMTVTAEEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCVDELNQKSDLAVTLETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3321..3611)
                     /locus_tag="V8955_RS00485"
                     /old_locus_tag="V8955_00485"
     CDS             complement(3321..3611)
                     /locus_tag="V8955_RS00485"
                     /old_locus_tag="V8955_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001514113.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     GETQLRAWLLHLLKTEVTRQADVSRIQPLIDELNALPKPVPKEVSENDQQA"
                     /name="hypothetical protein"
     gene            3782..5929
                     /gene="mobQ"
                     /locus_tag="V8955_RS00490"
                     /old_locus_tag="V8955_00490"
     CDS             3782..5929
                     /gene="mobQ"
                     /locus_tag="V8955_RS00490"
                     /old_locus_tag="V8955_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_011666798.1"
                     /translation="MAIYHLSMKILSRSKGYSAVASAAYRAGEKILDERTGVIHDYTRK
                     NGVASAVILTPANAPAWCANRAELWNAVEKAERRKNSQLAREFELAIPRELAQDAARET
                     VLNFVRENFVSRGMIADVAFHNLGGSNPHAHIMLSMRAITPDGFGEKVREWNDWTHAET
                     WRGSWADHANRALANAGYQEEIDHRSYERQGVEKTPGIHLGKSACAMEKWGIETERGEQ
                     NRLINRLNLEIQVSRDELRNSELTEPARRMADLLNIELPDNATSYILHDIIEALPTDSN
                     AAWKLTSKAMSMMADMQATRRRWEDLNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWV
                     APEYGREQEKIQSLSQRMEKLRQHHWKVEKQDIPASQKAFETQWQQWGASGLADLREQL
                     RKREEELRRKEQEETERRRAEQLRRNDNAVMDSGVLKAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRARIFESVDLMRDRSGYICERSEIGQRPPLQHVYSSATFEQ
                     LLTQVCQTWPQHTRDLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSMGGVVRLNGTLYPAFAVPTADNSQLVINALTDKGLRFA
                     GYGEAVNHDADSPARPAPELMQFHLKTWSEPLFAIVNTPEKQPDHLFRSLGFERTWDEW
                     KRDEYARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(6178..6879)
                     /locus_tag="V8955_RS00495"
                     /old_locus_tag="V8955_00495"
     CDS             complement(6178..6879)
                     /locus_tag="V8955_RS00495"
                     /old_locus_tag="V8955_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_011666799.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGSDAESTEL
                     QKTMTVTAEEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCVDELNQKSDLAVTLETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(7412..7702)
                     /locus_tag="V8955_RS00500"
                     /old_locus_tag="V8955_00500"
     CDS             complement(7412..7702)
                     /locus_tag="V8955_RS00500"
                     /old_locus_tag="V8955_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001514113.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     GETQLRAWLLHLLKTEVTRQADVSRIQPLIDELNALPKPVPKEVSENDQQA"
                     /name="hypothetical protein"
     Typing          complement(3616..3766)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.81"
                     /coverage="85.0"
                     /evalue="3.53e-55"
     Typing          complement(3783..4137)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7707..7857)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.81"
                     /coverage="85.0"
                     /evalue="3.53e-55"
     Typing          complement(7874..8182)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.1400000000000005e-161"
     Typing          complement(2089..2788)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.158"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(6180..6879)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.158"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4..1835
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3741..5926
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.863"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cagttctcaa cttcgtaagg gaaaactttg tcagtcgcgg catgatcgcc gacgtggcct
       61 ttcacaatct gggcgggtca aacccacacg cgcacatcat gctatccatg agggcaatca
      121 cgcctgacgg cttcggggag aaagtccgtg agtggaatga ctggactcac gccgaaacat
      181 ggcggggatc atgggcagac catgcgaaca gggcgctggc gaacgccggt tatcaggaag
      241 agatagacca tcgttcatac gagcgacagg gggttgagaa aacgcctggc attcacctcg
      301 gaaagtcggc ctgtgcgatg gaaaagtggg gcatcgaaac agaacgcggc gaacagaatc
      361 gcctgattaa caggcttaac ctggaaatac aggtttcccg cgacgaactc aggaacagtg
      421 agctgacaga gcccgcacgc cgtatggctg accttctgaa tattgagctt cctgacaacg
      481 caacttcgta catactgcat gacatcatcg aagcgttgcc gacggacagt aacgcagcat
      541 ggaaactcac cagtaaagcc atgtcgatga tggcagatat gcaggcgacg cgcagacgct
      601 gggaagatct gaacaccgaa cgaaaggaag cgatgagcca tgctgccagc ctgaaaaaat
      661 catcgccgtt ttcgtccggt ttttcccgta ttcccctgat gcaatgggtt gcaccggaat
      721 acggccgcga acaggaaaaa atccagtcgt tgagccagcg catggaaaaa ttgcggcagc
      781 atcactggaa ggttgagaaa caggacattc ccgcgagtca aaaagcattt gaaacacagt
      841 ggcagcagtg gggtgcatct ggccttgcag atctccgaga acagttgcgt aaacgtgaag
      901 aggaattgcg ccggaaggag caggaggaaa cagaacgtcg tcgggcggag cagctcagga
      961 gaaatgacaa tgccgttatg gattcagggg tactgaaagc agtcgttacg gggtatggcg
     1021 aggcccccat gccaggtaac ggggaaatga cctgttacct tctgcttcac aaccggaacg
     1081 gagaatatac gctctggggg aacgaactgg agaaataccg cgccagaatt tttgagagtg
     1141 ttgatttaat gcgtgacagg agtggttata tctgtgagcg aagcgaaatc ggtcaacgtc
     1201 caccactgca acatgtttat tcctctgcga cctttgagca actgctgacg caggtttgcc
     1261 agacatggcc gcaacatacc cgcgatctcc gccagccgaa gacatggccg gagtctttct
     1321 gtcttggaga agaccgccag ccagccatgc catcactggc ggcaaggaag gtggatttta
     1381 cgcagggacg tctgccaccg actctgatgc cggtaatgag ttctgtcgac cgtgaaaccc
     1441 gccagcttca gcttttgctg gtcatgggtg tggatgactc tatgggcggt gttgtccggc
     1501 ttaatggtac gctgtatccc gcgtttgccg tccccacagc cgacaacagc cagctggtaa
     1561 tcaatgcact gaccgacaaa ggtttacgct ttgcaggcta tggtgaagcg gtaaaccacg
     1621 atgctgacag tccggcacgt cctgcgccgg aacttatgca gtttcatctg aaaacctgga
     1681 gcgaaccgct ttttgctatc gtcaacacgc cagaaaaaca gccggatcat ctgttccgct
     1741 cgctgggctt tgagcgcacc tgggatgaat ggaaacgtga tgaatatgcc cgaacacacg
     1801 ccaccgagag gagacatgat cgcggttggt cacagtgaat gtgaccaata ttttgttttg
     1861 tttaacatcg tgttatgttc gtcgatgttc atcgacaggg atgaatgcaa gaagcacaca
     1921 cataacacaa atcaacttta tagttttccc gcccccgaaa aattgggggt tcttttttca
     1981 ctgtgccaga gcgtttatga cgcaattttt tacttcacca gtaccttcgc atgccaaaca
     2041 caaaaaaaac gcgtcagaga gcgttttaga ggccttaaaa tcgattctag atcgtcatct
     2101 tgatctgttt gtcttccttg aagcgaaaat gcagggctac aaccgtgcgc cccttcttga
     2161 tcgtctcaag cgttacagcc aagtcgcttt tttgattcag ttcgtcaacg caaggtctta
     2221 ataattgctg gttcagagac ttatatgttt gatatttatg ctctatatca agcattgagc
     2281 gaaaatcatc gagggcgata atacgttccc ctgtgctgcg gaattgctgg agaagttcgt
     2341 atattcttat gctgtaactt cttgataaat tggagatgtt cttaattaca actctagtaa
     2401 attgcccctt caactgtgtc agataaggca tgaccgcatc tgaaaaagtg atttgggctt
     2461 tgccctcacc tttagcgtac tgtgcccgat actggatcca gcggaactcc tcgcgcttct
     2521 catcgtcctt aaggatgatc gacctgtccc acaaacgatc aattgcttcc tgtagctgaa
     2581 cctcggcatt tttccgcccc atatcaggga atgagtcgaa atactcttcg gcggtaacag
     2641 tcatggtctt ttgcaattca gtggattctg catcagaacc cgacttcaat cgaccaatgc
     2701 aaagtaacaa gaatcgctgt tcctgaagag tcaatttata gctggcctca accagtgcat
     2761 tggatttgta ggcaatcaaa tccgacattg taaatccctc aacctaaggt aatgagtaaa
     2821 atataaacat tcctttaggc aaaggcaata tgatttaatt ttaatatccc ttttcctgtg
     2881 aatattacct taaatcatat tgcctttacc ttaaatcata ttgcctatcc accttaaatc
     2941 atattgcctt taccttaaat tatattacct atagtcttct aaactcttga atttcataag
     3001 aaaattcgac cctaaaacaa gtattaaaaa caagatctta aaaacaagat ctaaaaactt
     3061 aacggcgagc ctgtggaaaa cttggggtgt tcaaaacaaa aaatcatgaa aaacaacagg
     3121 ttacaaaaaa gcaggttcgc aagctttagc aaaaccccgc tcaaccctgt ggaaatccag
     3181 tattcatgcg ggttcagcgc atatcacctg tcgaaaaacc gtgcgtatgt gctcaatctg
     3241 gcgacggatt cacttccgct tcctcgccca ctcgctcgct aacgctcggt cgctggggct
     3301 gcggcgagcg gacggtgcat tcacgcctgt tggtcgtttt ctgacacttc tttggggact
     3361 ggttttggta acgcgttcag ttcgtcaatc aacggctgta tcctgctcac atcagcctgc
     3421 cgtgttacct ctgtcttcag gagatgaagc aaccaggctc gtagttgtgt ttccccaggc
     3481 ttcctgacct ttgccagcaa tgccgctccc acaatgacct tctgcgctgt gtccaggctc
     3541 ctgctcttcg ccttcagacg ctgcaatctg gcctcagcat cggcaatctt ctgttcgagt
     3601 gttctactca ttccgtaact ccgtgcgcgg tgaaaaatcg cattttagcg cgtcactggt
     3661 agtttaaaaa ctgaactggc ataatgcaca gcaaatcacg aagtgcgcac ttatacaaac
     3721 tctccttcgt cgagtttgtg tgcgccctgc ggggctaaaa gaaaacggca acaacgacac
     3781 gatggcaatt tatcatctca gcatgaagat tctttccaga agcaagggct acagcgccgt
     3841 tgcttctgct gcctatcgtg ccggagaaaa aatactggat gagcgcaccg gagtgattca
     3901 cgactacacc cgcaaaaacg gtgtagccag cgcagtgatt ctgacgccag caaacgcccc
     3961 agcatggtgt gcgaaccgcg ccgaactctg gaatgctgtt gaaaaagccg agcggcggaa
     4021 aaactcacaa cttgcgcggg agttcgaact cgccatcccc cgtgaactgg cgcaggatgc
     4081 cgcacgggaa acagttctca acttcgtaag ggaaaacttt gtcagtcgcg gcatgatcgc
     4141 cgacgtggcc tttcacaatc tgggcgggtc aaacccacac gcgcacatca tgctatccat
     4201 gagggcaatc acgcctgacg gcttcgggga gaaagtccgt gagtggaatg actggactca
     4261 cgccgaaaca tggcggggat catgggcaga ccatgcgaac agggcgctgg cgaacgccgg
     4321 ttatcaggaa gagatagacc atcgttcata cgagcgacag ggggttgaga aaacgcctgg
     4381 cattcacctc ggaaagtcgg cctgtgcgat ggaaaagtgg ggcatcgaaa cagaacgcgg
     4441 cgaacagaat cgcctgatta acaggcttaa cctggaaata caggtttccc gcgacgaact
     4501 caggaacagt gagctgacag agcccgcacg ccgtatggct gaccttctga atattgagct
     4561 tcctgacaac gcaacttcgt acatactgca tgacatcatc gaagcgttgc cgacggacag
     4621 taacgcagca tggaaactca ccagtaaagc catgtcgatg atggcagata tgcaggcgac
     4681 gcgcagacgc tgggaagatc tgaacaccga acgaaaggaa gcgatgagcc atgctgccag
     4741 cctgaaaaaa tcatcgccgt tttcgtccgg tttttcccgt attcccctga tgcaatgggt
     4801 tgcaccggaa tacggccgcg aacaggaaaa aatccagtcg ttgagccagc gcatggaaaa
     4861 attgcggcag catcactgga aggttgagaa acaggacatt cccgcgagtc aaaaagcatt
     4921 tgaaacacag tggcagcagt ggggtgcatc tggccttgca gatctccgag aacagttgcg
     4981 taaacgtgaa gaggaattgc gccggaagga gcaggaggaa acagaacgtc gtcgggcgga
     5041 gcagctcagg agaaatgaca atgccgttat ggattcaggg gtactgaaag cagtcgttac
     5101 ggggtatggc gaggccccca tgccaggtaa cggggaaatg acctgttacc ttctgcttca
     5161 caaccggaac ggagaatata cgctctgggg gaacgaactg gagaaatacc gcgccagaat
     5221 ttttgagagt gttgatttaa tgcgtgacag gagtggttat atctgtgagc gaagcgaaat
     5281 cggtcaacgt ccaccactgc aacatgttta ttcctctgcg acctttgagc aactgctgac
     5341 gcaggtttgc cagacatggc cgcaacatac ccgcgatctc cgccagccga agacatggcc
     5401 ggagtctttc tgtcttggag aagaccgcca gccagccatg ccatcactgg cggcaaggaa
     5461 ggtggatttt acgcagggac gtctgccacc gactctgatg ccggtaatga gttctgtcga
     5521 ccgtgaaacc cgccagcttc agcttttgct ggtcatgggt gtggatgact ctatgggcgg
     5581 tgttgtccgg cttaatggta cgctgtatcc cgcgtttgcc gtccccacag ccgacaacag
     5641 ccagctggta atcaatgcac tgaccgacaa aggtttacgc tttgcaggct atggtgaagc
     5701 ggtaaaccac gatgctgaca gtccggcacg tcctgcgccg gaacttatgc agtttcatct
     5761 gaaaacctgg agcgaaccgc tttttgctat cgtcaacacg ccagaaaaac agccggatca
     5821 tctgttccgc tcgctgggct ttgagcgcac ctgggatgaa tggaaacgtg atgaatatgc
     5881 ccgaacacac gccaccgaga ggagacatga tcgcggttgg tcacagtgaa tgtgaccaat
     5941 attttgtttt gtttaacatc gtgttatgtt cgtcgatgtt catcgacagg gatgaatgca
     6001 agaagcacac acataacaca aatcaacttt atagttttcc cgcccccgaa aaattggggg
     6061 ttcttttttc actgtgccag agcgtttatg acgcaatttt ttacttcacc agtaccttcg
     6121 catgccaaac acaaaaaaaa cgcgtcagag agcgttttag aggccttaaa atcgattcta
     6181 gatcgtcatc ttgatctgtt tgtcttcctt gaagcgaaaa tgcagggcta caaccgtgcg
     6241 ccccttcttg atcgtctcaa gcgttacagc caagtcgctt ttttgattca gttcgtcaac
     6301 gcaaggtctt aataattgct ggttcagaga cttatatgtt tgatatttat gctctatatc
     6361 aagcattgag cgaaaatcat cgagggcgat aatacgttcc cctgtgctgc ggaattgctg
     6421 gagaagttcg tatattctta tgctgtaact tcttgataaa ttggagatgt tcttaattac
     6481 aactctagta aattgcccct tcaactgtgt cagataaggc atgaccgcat ctgaaaaagt
     6541 gatttgggct ttgccctcac ctttagcgta ctgtgcccga tactggatcc agcggaactc
     6601 ctcgcgcttc tcatcgtcct taaggatgat cgacctgtcc cacaaacgat caattgcttc
     6661 ctgtagctga acctcggcat ttttccgccc catatcaggg aatgagtcga aatactcttc
     6721 ggcggtaaca gtcatggtct tttgcaattc agtggattct gcatcagaac ccgacttcaa
     6781 tcgaccaatg caaagtaaca agaatcgctg ttcctgaaga gtcaatttat agctggcctc
     6841 aaccagtgca ttggatttgt aggcaatcaa atccgacatt gtaaatccct caacctaagg
     6901 taatgagtaa aatataaaca ttcctttagg caaaggcaat atgatttaat tttaatatcc
     6961 cttttcctgt gaatattacc ttaaatcata ttgcctttac cttaaatcat attgcctatc
     7021 caccttaaat catattgcct ttaccttaaa ttatattacc tatagtcttc taaactcttg
     7081 aatttcataa gaaaattcga ccctaaaaca agtattaaaa acaaaatctt aaaaacaaaa
     7141 tctaaaaact taacggcgag cctgtggaaa acttggggtg ttcaaaacaa aaaatcatga
     7201 aaaacaacag gttacaaaaa agcaggttcg caagctttag caaaaccccg ctcaaccctg
     7261 tggaaatcca gtattcatgc gggttcagcg catatcacct gtcgaaaaac cgtgcgtatg
     7321 tgctcaatct ggcgacggat tcacttccgc ttcctcgccc actcgctcgc taacgctcgg
     7381 tcgctggggc tgcggcgagc ggacggtgca ttcacgcctg ttggtcgttt tctgacactt
     7441 ctttggggac tggttttggt aacgcgttca gttcgtcaat caacggctgt atcctgctca
     7501 catcagcctg ccgtgttacc tctgtcttca ggagatgaag caaccaggct cgtagttgtg
     7561 tttccccagg cttcctgacc tttgccagca atgccgctcc cacaatgacc ttctgcgctg
     7621 tgtccaggct cctgctcttc gccttcagac gctgcaatct ggcctcagca tcggcaatct
     7681 tctgttcgag tgttctactc attccgtaac tccgtgcgcg gtgaaaaatc gcattttagc
     7741 gcgtcactgg tagtttaaaa actgaactgg cataatgcac agcaaatcac gaagtgcgca
     7801 cttatacaaa ctctccttcg tcgagtttgt gtgcgccctg cggggctaaa agaaaacggc
     7861 aacaacgaca cgatggcaat ttatcatctc agcatgaaga ttctttccag aagcaagggc
     7921 tacagcgccg ttgcttctgc tgcctatcgt gccggagaaa aaatactgga tgagcgcacc
     7981 ggagtgattc acgactacac ccgcaaaaac ggtgtagcca gcgcagtgat tctgacgcca
     8041 gcaaacgccc cagcatggtg tgcgaaccgc gccgaactct ggaatgctgt tgaaaaagcc
     8101 gagcggcgga aaaactcaca acttgcgcgg gagttcgaac tcgccatccc ccgtgaactg
     8161 gcgcaggatg ccgcacggga aa
//
