LOCUS       NZ_CP146562            11262 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain B1.H1.18 plasmid pB1.H1.18.c4, complete
            sequence.
ACCESSION   NZ_CP146562
VERSION     NZ_CP146562.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40199719
            Assembly: GCF_037081895.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11262)
  AUTHORS   Rolbiecki,D.
  TITLE     Genomic and metagenomic analysis reveals shared resistance genes and
            mobile genetic elements in E. coli and Klebsiella spp. isolated from
            hospital patients and hospital wastewater at intra- and inter-genus
            level
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11262)
  AUTHORS   Rolbiecki,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-FEB-2024) Department of Water Protection Engineering
            and Environmental Microbiology, Uniwersytet Warminsko-Mazurski w
            Olsztynie, Prawochenskiego 1, Olsztyn 10-720, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: GS De Novo Assembler v. Flye 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 392x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037081895.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 17:58:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,239
            CDSs (total)                      :: 5,120
            Genes (coding)                    :: 4,773
            CDSs (with protein)               :: 4,773
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 347
            CDSs (without protein)            :: 347
            Pseudo Genes (ambiguous residues) :: 0 of 347
            Pseudo Genes (frameshifted)       :: 199 of 347
            Pseudo Genes (incomplete)         :: 160 of 347
            Pseudo Genes (internal stop)      :: 35 of 347
            Pseudo Genes (multiple problems)  :: 44 of 347
            Pseudo Genes (short protein)      :: 4 of 347
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP146562.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B1.H1.18"
                     /isolation_source="Hospital Wastewater"
                     /db_xref="taxon:562"
                     /plasmid="pB1.H1.18.c4"
                     /geo_loc_name="Poland:Olsztyn"
                     /lat_lon="53.77 N 20.48 E"
                     /collection_date="2021-04-20"
                     /collected_by="University of Warmia and Mazury in Olsztyn"
     gene            complement(join(10882..11262,1..672))
                     /locus_tag="V8955_RS00405"
                     /old_locus_tag="V8955_00405"
     CDS             complement(join(10882..11262,1..672))
                     /locus_tag="V8955_RS00405"
                     /old_locus_tag="V8955_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001053268.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     gene            complement(1153..1386)
                     /locus_tag="V8955_RS00410"
                     /old_locus_tag="V8955_00410"
     CDS             complement(1153..1386)
                     /locus_tag="V8955_RS00410"
                     /old_locus_tag="V8955_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137437.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1393..1923)
                     /locus_tag="V8955_RS00415"
                     /old_locus_tag="V8955_00415"
     CDS             complement(1393..1923)
                     /locus_tag="V8955_RS00415"
                     /old_locus_tag="V8955_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1961..2659)
                     /locus_tag="V8955_RS00420"
                     /old_locus_tag="V8955_00420"
                     /pseudo=""
     CDS             complement(<1961..2659)
                     /locus_tag="V8955_RS00420"
                     /old_locus_tag="V8955_00420"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2601..2924)
                     /locus_tag="V8955_RS00425"
                     /old_locus_tag="V8955_00425"
     CDS             complement(2601..2924)
                     /locus_tag="V8955_RS00425"
                     /old_locus_tag="V8955_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_046072959.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLCQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2989..3180
                     /locus_tag="V8955_RS00430"
                     /old_locus_tag="V8955_00430"
     CDS             2989..3180
                     /locus_tag="V8955_RS00430"
                     /old_locus_tag="V8955_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165975.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4383..5249)
                     /locus_tag="V8955_RS00435"
                     /old_locus_tag="V8955_00435"
     CDS             complement(4383..5249)
                     /locus_tag="V8955_RS00435"
                     /old_locus_tag="V8955_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(5251..6303)
                     /locus_tag="V8955_RS00440"
                     /old_locus_tag="V8955_00440"
     CDS             complement(5251..6303)
                     /locus_tag="V8955_RS00440"
                     /old_locus_tag="V8955_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001053268.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     gene            complement(6784..7017)
                     /locus_tag="V8955_RS00445"
                     /old_locus_tag="V8955_00445"
     CDS             complement(6784..7017)
                     /locus_tag="V8955_RS00445"
                     /old_locus_tag="V8955_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137437.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(7024..7554)
                     /locus_tag="V8955_RS00450"
                     /old_locus_tag="V8955_00450"
     CDS             complement(7024..7554)
                     /locus_tag="V8955_RS00450"
                     /old_locus_tag="V8955_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<7592..8290)
                     /locus_tag="V8955_RS00455"
                     /old_locus_tag="V8955_00455"
                     /pseudo=""
     CDS             complement(<7592..8290)
                     /locus_tag="V8955_RS00455"
                     /old_locus_tag="V8955_00455"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(8232..8555)
                     /locus_tag="V8955_RS00460"
                     /old_locus_tag="V8955_00460"
     CDS             complement(8232..8555)
                     /locus_tag="V8955_RS00460"
                     /old_locus_tag="V8955_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_046072959.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLCQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            8620..8811
                     /locus_tag="V8955_RS00465"
                     /old_locus_tag="V8955_00465"
     CDS             8620..8811
                     /locus_tag="V8955_RS00465"
                     /old_locus_tag="V8955_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165975.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(10014..10880)
                     /locus_tag="V8955_RS00470"
                     /old_locus_tag="V8955_00470"
     CDS             complement(10014..10880)
                     /locus_tag="V8955_RS00470"
                     /old_locus_tag="V8955_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     Typing          complement(3275..3356)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          complement(8906..8987)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          complement(3813..4000)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          complement(9444..9631)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          complement(1050..2611)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6681..8242)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agtcatgtta cgaatatccg ctaacgcttg ttcttgatct ttatagaaac ctttttctat
       61 cgccagcctc ataaaatgcc ccggggcatt tacagaaata agtattcccc ctggtattct
      121 tctatcatga tttcttgttc tatgagcaga agagtaaaaa acattaacac cagccttata
      181 tatttttagc ccatctttat cctgaaatgg tacgctttca gtataaataa catcctcttt
      241 tattggatag cactctggca tatgcagcga acaaatatat ttttgtatgt ccactaactc
      301 ttctgatgga cgagcaaagg caagttgtgg atgaataaaa taaatgagat gcgcactgac
      361 ctctcctctt cttgcgcgtt ctttaaaacg aactctttca ttacgcaaaa aatcaaacaa
      421 atgaccatca tcattatata gttgggggct atttacaata gataaatgga aatcaagccc
      481 tttaattttc ttgcttgcca atttaccaaa tacacagggc tgattgcctg acacccattt
      541 tcttagtaaa tcttcaattt tatctttaga agtttcgtta aagataaatg aatgaatatt
      601 atcaatatct tttccaaatg aaaaactgga tgataattgt ggtagtttat ttatacgttg
      661 ttgaagattc atgaaagctc ccacaagaca cctcatatat ttttttatat actgaaacta
      721 cattatattt attaccggcg taaaacgaaa taatttatta tcgttaccga tattttaaaa
      781 aaatcattat ttgcacagtg ttcgagttgt tagtatcact ccttcctgaa aatccgctcc
      841 atttaccata acgtcgcatt gtacgcaccg ccaaaacgcg ttcggtgcga tcacggcagc
      901 agacaggtaa aaatggcaac aaaccacccg aaaaactgcc gcgatcgcac ctgataaatt
      961 ttaaccgcat gaatacctat gcaaccagat gctacaggcc acattagccc gccttaatcc
     1021 actgaacacg tcatttttca tggtttcacc atcccagcga agggccatcc agtgtccgct
     1081 cctggatttc cggctgacgc tcccgctcca gagataactc atgttcgcgt tcctgcatca
     1141 gccgttcatc ccttatctcc tgttctcgct gtataactgg ctcaagcgtt ctgtccgctc
     1201 gctcaagtgc tgcacctgct gactcaactg catgactcgc ccgttcagca tcgcgttgtc
     1261 ccgttgcgta agcgaaaaca ttttctgcaa ttccacgaag gcgttctccc attcgttcag
     1321 ccgctgcata tagtcctgtt gcagttgctc taatgcgttc agcaaatgtc tttccagctc
     1381 cgtcatgctc tgtcactcct gcaccagtct cactgcgttc agtttgccgt tgtccttcgt
     1441 ccagttcgtt tcggctttca tccccttcgg caacaccaga aaacgtccgt tgctgtcctc
     1501 gtgataacgc acgccccatg tcttcgcgtt cagcttctcc agactctctt tctgctcgcg
     1561 gagaatattc atattcgcgt ttatcttcat gccctggtac cacagcagac tcccggtcgt
     1621 ggcaaacatc agtactgaga cgataatcag ccacagccag cgtttcacgc tcattttcag
     1681 tacggttttc cagtgcgcgt ccatcgtatc gacggtcgtt ttctgatacc tctccagcag
     1741 ttctttcagg gctgttgtat ggtcgttgat agcgttcctg attaaagatt cgtttgaggt
     1801 cagttccctg tttacagatt cttcgagctt cctgaactcg tcgttcagca ttttctctgt
     1861 agattcggct ctctctttca gctttttctc gaactccgcg cccgtctgta aaagattgct
     1921 cataaaacgc tcctttcagt ctgatattcc tcccgccgtt cgggtctgca atgctgatac
     1981 tgcttcgcgt caccctgacc acttcaagcc ccgcctctgt gagcgcctga atcacatcct
     2041 gacggccttt tatctctccg gcatggtaaa gcgcgtctat cccgcgtgtg acgccctcag
     2101 caagcgcctg tttcgtttcc ggcaggttat caggaagggt cagcgtccgg cggttctccg
     2161 gggcgttggg gtcatgcaga ccgtaatggt gattcaccag cgtctgccag gcatcaattc
     2221 tcggcctgtc tgcccggtcg tagtacggct ggaggcgttt tccggtctgt agctccatgt
     2281 tcggaatgac aaaattcagc tcaagccgtc ccttgtcctg gtgctccacc cacaggatgc
     2341 tgtactgatt tttctcgaga ccgggcatca gtacacgctc aaagctcgcc atcacttttt
     2401 cacgccctcc aggcggcagc tccttctccg cgaacgacag aacacctgac gtgtatttct
     2461 tcgcaaatgg cgtggcatcg atgagttccc ggacctcttc cggattcccc tgaagcaccg
     2521 ttgccccttc ccggttacgc tcccttccca gcaggtaatc aaccggacca ctgccaccac
     2581 cttttcccct ggcatgaaac ttaactatca tcccgcgccc cctgttccct gaccgccaga
     2641 cgcagccggc gcagctcatc cccgatggcc atcagtgcgg ccaccacctg aacccggtca
     2701 ccggaagacc actgcccgct gttcaccttc ctcgctgtct ggttaaggtt attcccgatg
     2761 gcggccagct gacacagtaa cggcggtgcc agtgtcggca gcttcccgga acgggcaacc
     2821 ggctcaccca ggcagacccg ccgcatccat accgccagtt gtttaccctc acagcgttcc
     2881 agtaaccggg catgttcatc atcagtaacc cgtatcgtga gcatcctctc gcgtttcatc
     2941 ggtatcatta cccccatgaa cagaaccccc ccttacacgg aggcatcagt gaccaaacag
     3001 gaaaaaaccg cccttaacat ggcccgcttc atcagaggcc agacattaac gctgctggag
     3061 aagctcaacg aactggacgc ggatgaacag gcagacatct gtgaatcgct tcacgaccac
     3121 gccgatgagc tttaccgcag ttgcctcgca cgcttcgggg atgacggtga aaaccgctga
     3181 cacatgcagc tcccggagac ggtcacagct tgtctgtgag cggatgccgg gagcagacaa
     3241 gcccgccagg gcgcgtcagg ccgttgacct ttggggtgtc ggggcgaagc cctgacccag
     3301 tcacgtagcg atagcggagt gtatactggc ttaaccattc ggcatcagtg cggattgtat
     3361 gaaaagtgcg ccatgtccgg tgtgaaatgc cgcacagatg cgtaaggaga aaatgccagt
     3421 tcaggcgctc ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cggctgcggc
     3481 gagcggactc tcctttctca aaaacgctaa ccctgttatc cacagaaccc ggggataaac
     3541 caagaaagaa catgtgcgca aaaaacgaaa ccccgggaaa ggccgcgccg gaggcgcttt
     3601 ttccataggc tccgcccccc tgacggactt cacaaaacag acactttttc ggtgcatctg
     3661 tgggagcctt gcggctcaac catgaaatcc gccagcggtg gcgaaacccg acgtggctta
     3721 aagatcatca ccgtttccgg tgaatcgctc cctcctgcgc ccccacgttc ccgccatatc
     3781 gattaccgga taccttttcc gcctttctcc cttacgggac acgtggcgct ttctcatagc
     3841 tcacgtcttc tggtatctcg gggcgactta ggcagttcgc tccgggctgg gctgtatgca
     3901 ggaacccccc gttcagcccg actgctgcgc cttctccggt aactgttctc ttgggtccaa
     3961 cccggaaaag acaccacaaa gcgccactgg cagcagccat tggtaaaagt catgtgcgag
     4021 atatttagag agtacttgaa gtggctgccg gagtgcggct acactagaag aacaattttg
     4081 gtgactcgtc tcgcataaga cagttacctg gttaagcagt ctgcaagctg acttaacctt
     4141 cgatcaaacc gccctgcaag gcggtttttt cgtttacagg cagcagatta cgcgcagaaa
     4201 aaaaggatct caagaggatc ctttgatctt tttgttacaa acgtctctat ttgactcccc
     4261 tgagagcgtt ttagcgcgcc tctgaccccc ttggttacat tccgacacca gaaacgctcc
     4321 cgttttttac gtgcatttgg cacggtatcg atggaggccg gggggcaatc ctgaacagaa
     4381 tgttactcct ctgagtattt tctgttaaga aaatatacac ttagcacagt tacaataaat
     4441 cctaccccct gtaagaatgt gaattgttca tccaaaaaca accatgacaa aaataatgca
     4501 actagcggaa taaagtacaa cattatccct gccaaatgaa ctgggagcca acgcaaggag
     4561 ttcaagtaaa aacaaaaagc taaaaaatat tgtaatattc cagatgaaaa aacaataatc
     4621 acggattcca acggaagata aattgatgat ttagatataa aaataaagag cgatgacagt
     4681 ataagggaga atgtcaactg tccgctcaat aatgtgatag gctcaaacga tttaataagg
     4741 cgagacgata taacaacata aaatgctgcc gataacacac cggcaacaac aagacaatat
     4801 ccaacaatat tattcgatcc attactgatg tctggcaagg ctaccaaaat actgccaaca
     4861 attgcaccta taaggaacaa tataaaatat tgcggggaat taattttaac tgagcaaata
     4921 agaacagaca acaccaatat aaacactgat tcaagtgaaa aaataattga tgcgcttcct
     4981 gctgaaataa aataaagtcc gtagagtgta aaagtatacg ctaagaatgg ttcaaaaacg
     5041 cctgtcgcat aagcaagtat cgcttttgag tttaatggga gtttttttct ttttataaaa
     5101 agaactatcc aagatagtat tactgcacta caaatctgcc agaaaaacac ccctccggca
     5161 tctagttctt caactccttt tttgctcatt acgatagctg ccccccatga gaacgctgct
     5221 aaaaaagcaa aaagcattcc tttaatcata ttaatcatcc tctttctcat aaaggggttt
     5281 gttttcaaat ttccttggtt gaaaggagtt aaaaaacata gatgcatcat ctacaggaat
     5341 accaataaac tctccataat aaggatcatc tataggaaac tcttctaatg aaacataaaa
     5401 caatacattc cagttgaaga tcattggatc actattttct atttcatgga gcaatctttc
     5461 atccttagtt aattctcctg gaattaatac atcagtatga taaaaaccgc tgtaataaga
     5521 actattaccg gtgtgcactg ggcatccaaa acgatccaaa ttagattctg aatgccatgt
     5581 tgtagaaatc ccttccggat gtgaataccc acccttacca acagactgaa tagtcatgtt
     5641 acgaatatcc gctaacgctt gttcttgatc tttatagaaa cctttttcta tcgccagcct
     5701 cataaaatgc cccggggcat ttacagaaat aagtattccc cctggtattc ttctatcatg
     5761 atttcttgtt ctatgagcag aagagtaaaa aacattaaca ccagccttat atatttttag
     5821 cccatcttta tcctgaaatg gtacgctttc agtataaata acatcctctt ttattggata
     5881 gcactctggc atatgcagcg aacaaatata tttttgtatg tccactaact cttctgatgg
     5941 acgagcaaag gcaagttgtg gatgaataaa ataaatgaga tgcgcactga cctctcctct
     6001 tcttgcgcgt tctttaaaac gaactctttc attacgcaaa aaatcaaaca aatgaccatc
     6061 atcattatat agttgggggc tatttacaat agataaatgg aaatcaagcc ctttaatttt
     6121 cttgcttgcc aatttaccaa atacacaggg ctgattgcct gacacccatt ttcttagtaa
     6181 atcttcaatt ttatctttag aagtttcgtt aaagataaat gaatgaatat tatcaatatc
     6241 ttttccaaat gaaaaactgg atgataattg tggtagttta tttatacgtt gttgaagatt
     6301 catgaaagct cccacaagac acctcatata tttttttata tactgaaact acattatatt
     6361 tattaccggc gtaaaacgaa ataatttatt atcgttaccg atattttaaa aaaatcatta
     6421 tttgcacagt gttcgagttg ttagtatcac tccttcctga aaatccgctc catttaccat
     6481 aacgtcgcat tgtacgcacc gccaaaacgc gttcggtgcg atcacggcag cagacaggta
     6541 aaaatggcaa caaaccaccc gaaaaactgc cgcgatcgca cctgataaat tttaaccgca
     6601 tgaataccta tgcaaccaga tgctacaggc cacattagcc cgccttaatc cactgaacac
     6661 gtcatttttc atggtttcac catcccagcg aagggccatc cagtgtccgc tcctggattt
     6721 ccggctgacg ctcccgctcc agagataact catgttcgcg ttcctgcatc agccgttcat
     6781 cccttatctc ctgttctcgc tgtataactg gctcaagcgt tctgtccgct cgctcaagtg
     6841 ctgcacctgc tgactcaact gcatgactcg cccgttcagc atcgcgttgt cccgttgcgt
     6901 aagcgaaaac attttctgca attccacgaa ggcgttctcc cattcgttca gccgctgcat
     6961 atagtcctgt tgcagttgct ctaatgcgtt cagcaaatgt ctttccagct ccgtcatgct
     7021 ctgtcactcc tgcaccagtc tcactgcgtt cagtttgccg ttgtccttcg tccagttcgt
     7081 ttcggctttc atccccttcg gcaacaccag aaaacgtccg ttgctgtcct cgtgataacg
     7141 cacgccccat gtcttcgcgt tcagcttctc cagactctct ttctgctcgc ggagaatatt
     7201 catattcgcg tttatcttca tgccctggta ccacagcaga ctcccggtcg tggcaaacat
     7261 cagtactgag acgataatca gccacagcca gcgtttcacg ctcattttca gtacggtttt
     7321 ccagtgcgcg tccatcgtat cgacggtcgt tttctgatac ctctccagca gttctttcag
     7381 ggctgttgta tggtcgttga tagcgttcct gattaaagat tcgtttgagg tcagttccct
     7441 gtttacagat tcttcgagct tcctgaactc gtcgttcagc attttctctg tagattcggc
     7501 tctctctttc agctttttct cgaactccgc gcccgtctgt aaaagattgc tcataaaacg
     7561 ctcctttcag tctgatattc ctcccgccgt tcgggtctgc aatgctgata ctgcttcgcg
     7621 tcaccctgac cacttcaagc cccgcctctg tgagcgcctg aatcacatcc tgacggcctt
     7681 ttatctctcc ggcatggtaa agcgcgtcta tcccgcgtgt gacgccctca gcaagcgcct
     7741 gtttcgtttc cggcaggtta tcaggaaggg tcagcgtccg gcggttctcc ggggcgttgg
     7801 ggtcatgcag accgtaatgg tgattcacca gcgtctgcca ggcatcaatt ctcggcctgt
     7861 ctgcccggtc gtagtacggc tggaggcgtt ttccggtctg tagctccatg ttcggaatga
     7921 caaaattcag ctcaagccgt cccttgtcct ggtgctccac ccacaggatg ctgtactgat
     7981 ttttctcgag accgggcatc agtacacgct caaagctcgc catcactttt tcacgccctc
     8041 caggcggcag ctccttctcc gcgaacgaca gaacacctga cgtgtatttc ttcgcaaatg
     8101 gcgtggcatc gatgagttcc cggacctctt ccggattccc ctgaagcacc gttgcccctt
     8161 cccggttacg ctcccttccc agcaggtaat caaccggacc actgccacca ccttttcccc
     8221 tggcatgaaa cttaactatc atcccgcgcc ccctgttccc tgaccgccag acgcagccgg
     8281 cgcagctcat ccccgatggc catcagtgcg gccaccacct gaacccggtc accggaagac
     8341 cactgcccgc tgttcacctt cctcgctgtc tggttaaggt tattcccgat ggcggccagc
     8401 tgacacagta acggcggtgc cagtgtcggc agcttcccgg aacgggcaac cggctcaccc
     8461 aggcagaccc gccgcatcca taccgccagt tgtttaccct cacagcgttc cagtaaccgg
     8521 gcatgttcat catcagtaac ccgtatcgtg agcatcctct cgcgtttcat cggtatcatt
     8581 acccccatga acagaacccc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc
     8641 gcccttaaca tggcccgctt catcagaggc cagacattaa cgctgctgga gaagctcaac
     8701 gaactggacg cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgccgatgag
     8761 ctttaccgca gttgcctcgc acgcttcggg gatgacggtg aaaaccgctg acacatgcag
     8821 ctcccggaga cggtcacagc ttgtctgtga gcggatgccg ggagcagaca agcccgccag
     8881 ggcgcgtcag gccgttgacc tttggggtgt cggggcgaag ccctgaccca gtcacgtagc
     8941 gatagcggag tgtatactgg cttaaccatt cggcatcagt gcggattgta tgaaaagtgc
     9001 gccatgtccg gtgtgaaatg ccgcacagat gcgtaaggag aaaatgccag ttcaggcgct
     9061 cttccgcttc ctcgctcact gactcgctac gctcggtcgt tcggctgcgg cgagcggact
     9121 ctcctttctc aaaaacgcta accctgttat ccacagaacc cggggataaa ccaagaaaga
     9181 acatgtgcgc aaaaaacgaa accccgggaa aggccgcgcc ggaggcgctt tttccatagg
     9241 ctccgccccc ctgacggact tcacaaaaca gacacttttt cggtgcatct gtgggagcct
     9301 tgcggctcaa ccatgaaatc cgccagcggt ggcgaaaccc gacgtggctt aaagatcatc
     9361 accgtttccg gtgaatcgct ccctcctgcg cccccacgtt cccgccatat cgattaccgg
     9421 ataccttttc cgcctttctc ccttacggga cacgtggcgc tttctcatag ctcacgtctt
     9481 ctggtatctc ggggcgactt aggcagttcg ctccgggctg ggctgtatgc aggaaccccc
     9541 cgttcagccc gactgctgcg ccttctccgg taactgttct cttgggtcca acccggaaaa
     9601 gacaccacaa agcgccactg gcagcagcca ttggtaaaag tcatgtgcga gatatttaga
     9661 gagtacttga agtggctgcc ggagtgcggc tacactagaa gaacaatttt ggtgactcgt
     9721 ctcgcataag acagttacct ggttaagcag tctgcaagct gacttaacct tcgatcaaac
     9781 cgccctgcaa ggcggttttt tcgtttacag gcagcagatt acgcgcagaa aaaaaggatc
     9841 tcaagaggat cctttgatct ttttgttaca aacgtctcta tttgactccc ctgagagcgt
     9901 tttagcgcgc ctctgacccc cttggttaca ttccgacacc agaaacgctc ccgtttttta
     9961 cgtgcatttg gcacggtatc gatggaggcc ggggggcaat cctgaacaga atgttactcc
    10021 tctgagtatt ttctgttaag aaaatataca cttagcacag ttacaataaa tcctaccccc
    10081 tgtaagaatg tgaattgttc atccaaaaac aaccatgaca aaaataatgc aactagcgga
    10141 ataaagtaca acattatccc tgccaaatga actgggagcc aacgcaagga gttcaagtaa
    10201 aaacaaaaag ctaaaaaata ttgtaatatt ccagatgaaa aaacaataat cacggattcc
    10261 aacggaagat aaattgatga tttagatata aaaataaaga gcgatgacag tataagggag
    10321 aatgtcaact gtccgctcaa taatgtgata ggctcaaacg atttaataag gcgagacgat
    10381 ataacaacat aaaatgctgc cgataacaca ccggcaacaa caagacaata tccaacaata
    10441 ttattcgatc cattactgat gtctggcaag gctaccaaaa tactgccaac aattgcacct
    10501 ataaggaaca atataaaata ttgcggggaa ttaattttaa ctgagcaaat aagaacagac
    10561 aacaccaata taaacactga ttcaagtgaa aaaataattg atgcgcttcc tgctgaaata
    10621 aaataaagtc cgtagagtgt aaaagtatac gctaagaatg gttcaaaaac gcctgtcgca
    10681 taagcaagta tcgcttttga gtttaatggg agtttttttc tttttataaa aagaactatc
    10741 caagatagta ttactgcact acaaatctgc cagaaaaaca cccctccggc atctagttct
    10801 tcaactcctt ttttgctcat tacgatagct gccccccatg agaacgctgc taaaaaagca
    10861 aaaagcattc ctttaatcat attaatcatc ctctttctca taaaggggtt tgttttcaaa
    10921 tttccttggt tgaaaggagt taaaaaacat agatgcatca tctacaggaa taccaataaa
    10981 ctctccataa taaggatcat ctataggaaa ctcttctaat gaaacataaa acaatacatt
    11041 ccagttgaag atcattggat cactattttc tatttcatgg agcaatcttt catccttagt
    11101 taattctcct ggaattaata catcagtatg ataaaaaccg ctgtaataag aactattacc
    11161 ggtgtgcact gggcatccaa aacgatccaa attagattct gaatgccatg ttgtagaaat
    11221 cccttccgga tgtgaatacc cacccttacc aacagactga at
//
