LOCUS       NZ_CP146560            10328 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain B1.H1.18 plasmid pB1.H1.18.c7, complete
            sequence.
ACCESSION   NZ_CP146560
VERSION     NZ_CP146560.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40199719
            Assembly: GCF_037081895.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10328)
  AUTHORS   Rolbiecki,D.
  TITLE     Genomic and metagenomic analysis reveals shared resistance genes and
            mobile genetic elements in E. coli and Klebsiella spp. isolated from
            hospital patients and hospital wastewater at intra- and inter-genus
            level
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10328)
  AUTHORS   Rolbiecki,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-FEB-2024) Department of Water Protection Engineering
            and Environmental Microbiology, Uniwersytet Warminsko-Mazurski w
            Olsztynie, Prawochenskiego 1, Olsztyn 10-720, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: GS De Novo Assembler v. Flye 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 392x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037081895.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 17:58:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,239
            CDSs (total)                      :: 5,120
            Genes (coding)                    :: 4,773
            CDSs (with protein)               :: 4,773
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 347
            CDSs (without protein)            :: 347
            Pseudo Genes (ambiguous residues) :: 0 of 347
            Pseudo Genes (frameshifted)       :: 199 of 347
            Pseudo Genes (incomplete)         :: 160 of 347
            Pseudo Genes (internal stop)      :: 35 of 347
            Pseudo Genes (multiple problems)  :: 44 of 347
            Pseudo Genes (short protein)      :: 4 of 347
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP146560.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B1.H1.18"
                     /isolation_source="Hospital Wastewater"
                     /db_xref="taxon:562"
                     /plasmid="pB1.H1.18.c7"
                     /geo_loc_name="Poland:Olsztyn"
                     /lat_lon="53.77 N 20.48 E"
                     /collection_date="2021-04-20"
                     /collected_by="University of Warmia and Mazury in Olsztyn"
     gene            293..1207
                     /locus_tag="V8955_RS00035"
                     /old_locus_tag="V8955_00035"
     CDS             293..1207
                     /locus_tag="V8955_RS00035"
                     /old_locus_tag="V8955_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271363.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(1411..1809)
                     /locus_tag="V8955_RS00040"
                     /old_locus_tag="V8955_00040"
     CDS             complement(1411..1809)
                     /locus_tag="V8955_RS00040"
                     /old_locus_tag="V8955_00040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            1986..2912
                     /locus_tag="V8955_RS00045"
                     /old_locus_tag="V8955_00045"
     CDS             1986..2912
                     /locus_tag="V8955_RS00045"
                     /old_locus_tag="V8955_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            2915..3448
                     /locus_tag="V8955_RS00050"
                     /old_locus_tag="V8955_00050"
     CDS             2915..3448
                     /locus_tag="V8955_RS00050"
                     /old_locus_tag="V8955_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(3514..3723)
                     /locus_tag="V8955_RS00055"
                     /old_locus_tag="V8955_00055"
     CDS             complement(3514..3723)
                     /locus_tag="V8955_RS00055"
                     /old_locus_tag="V8955_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            5457..6371
                     /locus_tag="V8955_RS00060"
                     /old_locus_tag="V8955_00060"
     CDS             5457..6371
                     /locus_tag="V8955_RS00060"
                     /old_locus_tag="V8955_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271363.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(6575..6973)
                     /locus_tag="V8955_RS00065"
                     /old_locus_tag="V8955_00065"
     CDS             complement(6575..6973)
                     /locus_tag="V8955_RS00065"
                     /old_locus_tag="V8955_00065"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            7150..8076
                     /locus_tag="V8955_RS00070"
                     /old_locus_tag="V8955_00070"
     CDS             7150..8076
                     /locus_tag="V8955_RS00070"
                     /old_locus_tag="V8955_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            8079..8612
                     /locus_tag="V8955_RS00075"
                     /old_locus_tag="V8955_00075"
     CDS             8079..8612
                     /locus_tag="V8955_RS00075"
                     /old_locus_tag="V8955_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(8678..8887)
                     /locus_tag="V8955_RS00080"
                     /old_locus_tag="V8955_00080"
     CDS             complement(8678..8887)
                     /locus_tag="V8955_RS00080"
                     /old_locus_tag="V8955_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     Typing          294..1207
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.909"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5458..6371
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.909"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1987..2909
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7151..8073
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gttatgcaac gccctgaaaa cgattctgac gcgtttttct ggtttggcct ggtgttttct
       61 tgtctttttg cgttttttgc gccagaaagc gactgagggc gttttaaggg gtacgtacaa
      121 cgggagttat ggtaaatgga tcggatttgc gggaaggctc gacaggtttt gtcgttgggg
      181 gtagtgtaag cgactgaaaa acaaacgccc ctgaaattcg tgtctatccg ccaagacttt
      241 cacgaaaata caggggcgat gtcggtcgat aacctggaag gaatcgtaac acatgagcgc
      301 cgcgcttcaa tacttcgaag aaaatttacc ccaccgtccc tatcacacgg atgatctcgc
      361 tttcggcctt cgtatctccg gcaaagggcg cgcgcttctt gcacggtata tccagcagaa
      421 ccagcctcat gcacagttct ggctggtttt tgacgttgac cgcgaggggg ctgcgattga
      481 ctggagcgac cggaacgctc ccgcgccgaa catcaccgtt aaaaatcctg tgaacggaca
      541 cgctcacctg ctctacgcac tcaacatcgc cgtgagaacc gcgcctgatg cctcggttaa
      601 ggcgctgaaa tatgccgcag cggttgaacg ttcgctgtgt gagaaacttt gtgcggatgt
      661 gaactacagc ggcctgattt gcaaaaatcc gttccacctg gaatggctgg tgatggagtg
      721 gcgcgaggaa gcctataccc tcgatgaact ggctgattat ctcgatttga gcgcctcaga
      781 gcgtcgtagc atcgataaac attacgggat ggggcgaaac tgccacctgt tcgaaatgac
      841 gcgcaaatgg gcttacaggg cgattcgtca gggctggcca gcgttctcac agtggcttga
      901 agcggtgatc cagcgtgtcg aaatgtacaa tgcatcgctt cccgttccgc tttcactggc
      961 tgaatgtcgg gctattggca agagcattgc gaaatacacg cacaggaact tcacgccgga
     1021 aactttcgca cagtatgtgg ctgatacgca cacgccagaa attcaggctg cacgcggtcg
     1081 caagggcggt tctaagtcta agcgcagcac agtagctaca tctgcacgca cgctgaaacc
     1141 gtgggaggca ttagggatca gtcgcgcctg gtattaccaa ctgaaaaaac gaggtctcgt
     1201 agagtagacc aaataagcct atatcagata acagcgcctt tctggcgtct ttttgagcag
     1261 caggtttttt gccgtttttc cccgttcagc cctgcaatgg cgcggctttc cgcgtgattg
     1321 tggtggtagt gctcgccgca gtctcatgac cgagcgtagc gagcgaatga gcgaggaagc
     1381 accagggaat cggaacacgg atggaggcac ttacgcgtct gggtttgggg atgtctccag
     1441 atcgtactta agttccttaa gtgcttgtaa tatcctgtct tttttatagc atagctcgcc
     1501 tgtttttgtg tctaccagtc ctgcttcaat cagtagccca gccgcgagaa agagccggtg
     1561 attgcgttcc cgtcgttgtt tttttccctc gattcgtttt tggaccgcct gttttcgttc
     1621 gctctcctgt gcctgtaagt cgtttttttc cgctgttgcc agtacgtgga gaagtttgcg
     1681 ttcctgttct gtgagtccgg cttcgttgtc gatcaggtca ataatctgtc tctgtcgtgc
     1741 ggtctgttct ccgagcttcc tgatgaatcc gatccgtttc ttcagccatt cgatgttgct
     1801 gatgcgcatg ttcgtgatgc tccctggtgt ttttttgcgg tgtgttgtgg tcgtgcagta
     1861 tatcgcttgc ggtcggcggg tgcagggggt atcttccgtg ctaattcgta aaattagaag
     1921 gaagcgcact tatatgtttc tctccttcgt cgagaaacag tgcgctcctg cagagggctg
     1981 aaaccgtggc aacctatcac ctcagcgtta aatttggtgg caaggggcag gctgcaaacc
     2041 acgccgatta catcgagcgc aaagagaaat accgcgaccg tcaggatttg gaatattccg
     2101 cacacggaaa catgccggaa tgggccaggg ataatccttc ccatttctgg caggcggctg
     2161 accagtttga acgtgcgaac ggttcgacct atagggaact tgaaattgca ttaccccgtg
     2221 aactgacacc ggaacagcgc cttgaactgg tgcaggattt tgtacgtcag gaagctggcg
     2281 aacggcacgc ctggagtttt gccattcaca atccgaaagc gagcattgac ggcggcgaac
     2341 agcctcatgc gcatatcatg atgagtcagc gtgtgaatga tggcattgaa cgtacaccgg
     2401 aacagtattt ccggcgctat aacgccaggt atcccgagcg gggaggtgcg aaaaaggatt
     2461 ccggaagcct gactctcact cagcaaaaag agcagttgcg ggaattgcga aaacgctggg
     2521 aggtgaagca caacgaacat atgagaaagc atggctttga aaggggcttc attgactgcc
     2581 ggacgctgaa agaacagggc attgaacgac gtcccgaagt gcatctgggt tttgaagctg
     2641 ccggacggct tgatgatgcc cagcgtcatg acatcatgca aaaacggtct gaaccggact
     2701 attcccgcca tcttgaacag gcgcgaacgg tcgggcatga catcagggaa tggctggaca
     2761 ctccggttaa gcaacatgaa ctggtcagaa ggctgcaaca tgatgttgcg cgggagcatg
     2821 gctttgacat gagagacaga gtgagcagac agaaccgaac tgcacctgaa cgggttaaat
     2881 caccgtcacg tggattttct atgggattct gaacatggcc agcacagaag aaaaattact
     2941 cacgctcctg ttatcggtgg agcatctgca acataccgca atggagcaac agaaagctgt
     3001 tgctgattct ctggctgtgg caagggaaac tcttcgcgga tatcgggata tttctgacgg
     3061 tgtggcaaaa caggtcaggg atgaagtcag aaatgaggtg cagaagctgg acgtggcagg
     3121 actgataggt gagcgcatca gccaggattt tcagcggctg gaagagtcag tcgcaaacat
     3181 gaaggcaaac gcggatgccc tgaacaggga tattgcgaca attcacagca atatcgtgca
     3241 ggaatacaac acccttaaag ggtatagctg gaaatggctg ggtggggtgt cagtggcttt
     3301 tctggttatg ttctgtgtgc tcggatgggg aatgaaaaca atactcgaca ggaactacaa
     3361 cgccacagcg gcggtgtatc agaaagttgt gacactggaa gataaactgg tggagaaacc
     3421 tgtcacgggg aagagccgga aaaactgacg cgcttcgctt gtccaacgcc tcacgacccg
     3481 cgaaaggatt tcgcgaaccg ttcaccgttt ttttcaatat gattctgtct gctttggtcg
     3541 ataaagcaga cgatagtatt ccagccttcg tatatataac ggtacgtgct catcagtaag
     3601 agctggcggt attgtgttat cagggggaag ttttttcaga tactcacgaa atgcctggct
     3661 tgatgccata aaatctacag tagcatcgat aatatgtggg gtgttttcac ctaattttgc
     3721 catgatgata atccttatat tatcacggtt tgtgaatgat agggtgtcac tattatttat
     3781 gcgttagtga accagaaaaa atcccccgtt ggcatagggg gatatgtaag gagaaataac
     3841 ttcgcaggtg ttcttgtttg tataacgtga aaagactgca cgcaacttaa taagtctgcc
     3901 gtacttactt tcagttttca acataaggtt tataacaatg tatggaatgg aagtgagctt
     3961 taaggtttca gctctaaaaa ataaaatatt tattagttgg ctggttgttg ttcttgttct
     4021 gtatgtatat aaacactatc gacatattat gaccgggaac attccttacc ccctctgcgc
     4081 tcgcagaggt tttttttgtc attaattaat aatgaactga aatgcagttc cgatacctcc
     4141 atttgcacat tatccctgac atatggtacc ggaaccgcat taattttatg ttataagagt
     4201 ttatttttaa tgtccagatg gtttaatagt atttatatgt aattagctgt atggatagta
     4261 ctggttttat tgtataactt gtcgtttttt tttattgttt ttgtgtggtg tgctaatctt
     4321 ttagttataa aaaaacccct tggaatacag gggcaaaagt aaggaaaaca aatggatgct
     4381 cagtttgtgt gacactttaa actaatggaa ctatagagtg gaggttgtta cagtcgcttt
     4441 tataaaacag caaccagttt ttttcaatag tctgatgatg gttgtgacag ttttgtgata
     4501 ttgttcaaaa tgtttcttgt tgaatgtttg ttttttggtc tattggtgtc tggttttatt
     4561 ttttctgcta ttacatatca agaaatgtga ttggtgtagc aagagtggaa tgcgatgtgg
     4621 ataaactaat tggttgtcga atctaattta cttagaaaac tacacttgtt tttgtgtatg
     4681 aatttagtga atttgtctgt ctatgtgttt tcgtgcattt ataaatcctt cgtcaggatt
     4741 ttaaatttag tgttgaatat gtagggcctg tacggtaatt attatttagc gtagctactt
     4801 catcgttgtt cgaacacaac caacaacctg taattgttgt ttctcttggt tccccacttt
     4861 tgtggggttt tttatctctt ccgtgcatcg taaagcgcgg actccttctg gaaaggttgt
     4921 tttagggcat cactgaggca ttttttgcgt ttgtgaatat tgagcatccg ataactaaaa
     4981 tatttcccat agactctgat tcttgccacc gaccccaggt cggttttttt tgtcctggcg
     5041 agatgtgaag tttggaaaca ggaaaccccg tcagagcgcc acgcaccagg ggagaaaact
     5101 tgtcgttttg agccgttcgg tgttcagaac gcacgaaacc gatcgcacat cgctttcgtg
     5161 catagttatg caacgccctg aaaacgattc tgacgcgttt ttctggtttg gcctggtgtt
     5221 ttcttgtctt tttgcgtttt ttgcgccaga aagcgactga gggcgtttta aggggtacgt
     5281 acaacgggag ttatggtaaa tggatcggat ttgcgggaag gctcgacagg ttttgtcgtt
     5341 gggggtagtg taagcgactg aaaaacaaac gcccctgaaa ttcgtgtcta tccgccaaga
     5401 ctttcacgaa aatacagggg cgatgtcggt cgataacctg gaaggaatcg taacacatga
     5461 gcgccgcgct tcaatacttc gaagaaaatt taccccaccg tccctatcac acggatgatc
     5521 tcgctttcgg ccttcgtatc tccggcaaag ggcgcgcgct tcttgcacgg tatatccagc
     5581 agaaccagcc tcatgcacag ttctggctgg tttttgacgt tgaccgcgag ggggctgcga
     5641 ttgactggag cgaccggaac gctcccgcgc cgaacatcac cgttaaaaat cctgtgaacg
     5701 gacacgctca cctgctctac gcactcaaca tcgccgtgag aaccgcgcct gatgcctcgg
     5761 ttaaggcgct gaaatatgcc gcagcggttg aacgttcgct gtgtgagaaa ctttgtgcgg
     5821 atgtgaacta cagcggcctg atttgcaaaa atccgttcca cctggaatgg ctggtgatgg
     5881 agtggcgcga ggaagcctat accctcgatg aactggctga ttatctcgat ttgagcgcct
     5941 cagagcgtcg tagcatcgat aaacattacg ggatggggcg aaactgccac ctgttcgaaa
     6001 tgacgcgcaa atgggcttac agggcgattc gtcagggctg gccagcgttc tcacagtggc
     6061 ttgaagcggt gatccagcgt gtcgaaatgt acaatgcatc gcttcccgtt ccgctttcac
     6121 tggctgaatg tcgggctatt ggcaagagca ttgcgaaata cacgcacagg aacttcacgc
     6181 cggaaacttt cgcacagtat gtggctgata cgcacacgcc agaaattcag gctgcacgcg
     6241 gtcgcaaggg cggttctaag tctaagcgca gcacagtagc tacatctgca cgcacgctga
     6301 aaccgtggga ggcattaggg atcagtcgcg cctggtatta ccaactgaaa aaacgaggtc
     6361 tcgtagagta gaccaaataa gcctatatca gataacagcg cctttctggc gtctttttga
     6421 gcagcaggtt ttttgccgtt tttccccgtt cagccctgca atggcgcggc tttccgcgtg
     6481 attgtggtgg tagtgctcgc cgcagtctca tgaccgagcg tagcgagcga atgagcgagg
     6541 aagcaccagg gaatcggaac acggatggag gcacttacgc gtctgggttt ggggatgtct
     6601 ccagatcgta cttaagttcc ttaagtgctt gtaatatcct gtctttttta tagcatagct
     6661 cgcctgtttt tgtgtctacc agtcctgctt cgatcagtag cccagccgcg agaaagagcc
     6721 ggtgattgcg ttcccgtcgt tgtttttttc cctcgattcg tttttggacc gcctgttttc
     6781 gttcgctctc ctgtgcctgt aagtcgtttt tttccgctgt tgccagtacg tggagaagtt
     6841 tgcgttcctg ttctgtgagt ccggcttcgt tgtcgatcag gtcaataatc tgtctctgtc
     6901 gtgcggtctg ttctccgagc ttcctgatga atccgatccg tttcttcagc cattcgatgt
     6961 tgctgatgcg catgttcgtg atgctccctg gtgttttttt gcggtgtgtt gtggtcgtgc
     7021 agtatatcgc ttgcggtcgg cgggtgcagg gggtatcttc cgtgctaatt cgtaaaatta
     7081 gaaggaagcg cacttatatg tttctctcct tcgtcgagaa acagtgcgct cctgcagagg
     7141 gctgaaaccg tggcaaccta tcacctcagc gttaaatttg gtggcaaggg gcaggctgca
     7201 aaccacgccg attacatcga gcgcaaagag aaataccgcg accgtcagga tttggaatat
     7261 tccgcacacg gaaacatgcc ggaatgggcc agggataatc cttcccattt ctggcaggcg
     7321 gctgaccagt ttgaacgtgc gaacggttcg acctataggg aacttgaaat tgcattaccc
     7381 cgtgaactga caccggaaca gcgccttgaa ctggtgcagg attttgtacg tcaggaagct
     7441 ggcgaacggc acgcctggag ttttgccatt cacaatccga aagcgagcat tgacggcggc
     7501 gaacagcctc atgcgcatat catgatgagt cagcgtgtga atgatggcat tgaacgtaca
     7561 ccggaacagt atttccggcg ctataacgcc aggtatcccg agcggggagg tgcgaaaaag
     7621 gattccggaa gcctgactct cactcagcaa aaagagcagt tgcgggaatt gcgaaaacgc
     7681 tgggaggtga agcacaacga acatatgaga aagcatggct ttgaaagggg cttcattgac
     7741 tgccggacgc tgaaagaaca gggcattgaa cgacgtcccg aagtgcatct gggttttgaa
     7801 gctgccggac ggcttgatga tgcccagcgt catgacatca tgcaaaaacg gtctgaaccg
     7861 gactattccc gccatcttga acaggcgcga acggtcgggc atgacatcag ggaatggctg
     7921 gacactccgg ttaagcaaca tgaactggtc agaaggctgc aacatgatgt tgcgcgggag
     7981 catggctttg acatgagaga cagagtgagc agacagaacc gaactgcacc tgaacgggtt
     8041 aaatcaccgt cacgtggatt ttctatggga ttctgaacat ggccagcaca gaagaaaaat
     8101 tactcacgct cctgttatcg gtggagcatc tgcaacatac cgcaatggag caacagaaag
     8161 ctgttgctga ttctctggct gtggcaaggg aaactcttcg cggatatcgg gatatttctg
     8221 acggtgtggc aaaacaggtc agggatgaag tcagaaatga ggtgcagaag ctggacgtgg
     8281 caggactgat aggtgagcgc atcagccagg attttcagcg gctggaagag tcagtcgcaa
     8341 acatgaaggc aaacgcggat gccctgaaca gggatattgc gacaattcac agcaatatcg
     8401 tgcaggaata caacaccctt aaagggtata gctggaaatg gctgggtggg gtgtcagtgg
     8461 cttttctggt tatgttctgt gtgctcggat ggggaatgaa aacaatactc gacaggaact
     8521 acaacgccac agcggcggtg tatcagaaag ttgtgacact ggaagataaa ctggtggaga
     8581 aacctgtcac ggggaagagc cggaaaaact gacgcgcttc gcttgtccaa cgcctcacga
     8641 cccgcgaaag gatttcgcga accgttcacc gtttttttca atatgattct gtctgctttg
     8701 gtcgataaag cagacgatag tattccagcc ttcgtatata taacggtacg tgctcatcag
     8761 taagagctgg cggtattgtg ttatcagggg gaagtttttt cagatactca cgaaatgcct
     8821 ggcttgatgc cataaaatct acagtagcat cgataatatg tggggtgttt tcacctaatt
     8881 ttgccatgat gataatcctt atattatcac ggtttgtgaa tgatagggtg tcactattat
     8941 ttatgcgtta gtgaaccaga aaaaatcccc cgttggcata gggggatatg taaggagaaa
     9001 taacttcgca ggtgttcttg tttgtataac gtgaaaagac tgcacgcaac ttaataagtc
     9061 tgccgtactt actttcagtt ttcaacataa ggtttataac aatgtatgga atggaagtga
     9121 gctttaaggt ttcagctcta aaaaataaaa tatttattag ttggctggtt gttgttcttg
     9181 ttctgtatgt atataaacac tatcgacata ttatgaccgg gaacattcct taccccctct
     9241 gcgctcgcag aggttttttt tgtcattaat taataatgaa ctgaaatgca gttccgatac
     9301 ctccatttgc acattatccc tgacatatgg taccggaacc gcattaattt tatgttataa
     9361 gagtttattt ttaatgtcca gatggtttaa tagtatttat atgtaattag ctgtatggat
     9421 agtactggtt ttattgtata acttgtcgtt tttttttatt gtttttgtgt ggtgtgctaa
     9481 tcttttagtt ataaaaaaac cccttggaat acaggggcaa aagtaaggaa aacaaatgga
     9541 tgctcagttt gtgtgacact ttaaactaat ggaactatag agtggaggtt gttacagtcg
     9601 cttttataaa acagcaacca gtttttttca atagtctgat gatggttgtg acagttttgt
     9661 gatattgttc aaaatgtttc ttgttgaatg tttgtttttt ggtctattgg tgtctggttt
     9721 tattttttct gctattacat atcaagaaat gtgattggtg tagcaagagt ggaatgcgat
     9781 gtggataaac taattggttg tcgaatctaa tttacttaga aaactacact tgtttttgtg
     9841 tatgaattta gtgaatttgt ctgtctatgt gttttcgtgc atttataaat ccttcgtcag
     9901 gatcttaaat ttagtgttga atatgtaggg cctgtacggt aattattatt tagcgtagct
     9961 acttcatcgt tgttcgaaca caaccaacaa cctgtaattg ttgtttctct tggttcccca
    10021 cttttgtggg gttttttatc tcttccgtgc atcgtaaagc gcggactcct tctggaaagg
    10081 ttgttttagg gcatcactga ggcatttttt gcgtttgtga atattgagca tccgataact
    10141 aaaatatttc ccatagactc tgattcttgc caccgacccc aggtcggttt tttttgtcct
    10201 ggcgagatgt gaagtttgga aacaggaaac cccgtcagag cgccacgcac caggggagaa
    10261 aacttgtcgt tttgagccgt tcggtgttca gaacgcacga aaccgatcgc acatcgcttt
    10321 cgtgcata
//
