LOCUS       NZ_CP146559             3868 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain B1.H1.18 plasmid pB1.H1.18.c8, complete
            sequence.
ACCESSION   NZ_CP146559
VERSION     NZ_CP146559.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40199719
            Assembly: GCF_037081895.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Rolbiecki,D.
  TITLE     Genomic and metagenomic analysis reveals shared resistance genes and
            mobile genetic elements in E. coli and Klebsiella spp. isolated from
            hospital patients and hospital wastewater at intra- and inter-genus
            level
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Rolbiecki,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-FEB-2024) Department of Water Protection Engineering
            and Environmental Microbiology, Uniwersytet Warminsko-Mazurski w
            Olsztynie, Prawochenskiego 1, Olsztyn 10-720, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: GS De Novo Assembler v. Flye 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 392x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037081895.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 17:58:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,239
            CDSs (total)                      :: 5,120
            Genes (coding)                    :: 4,773
            CDSs (with protein)               :: 4,773
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 347
            CDSs (without protein)            :: 347
            Pseudo Genes (ambiguous residues) :: 0 of 347
            Pseudo Genes (frameshifted)       :: 199 of 347
            Pseudo Genes (incomplete)         :: 160 of 347
            Pseudo Genes (internal stop)      :: 35 of 347
            Pseudo Genes (multiple problems)  :: 44 of 347
            Pseudo Genes (short protein)      :: 4 of 347
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP146559.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B1.H1.18"
                     /isolation_source="Hospital Wastewater"
                     /db_xref="taxon:562"
                     /plasmid="pB1.H1.18.c8"
                     /geo_loc_name="Poland:Olsztyn"
                     /lat_lon="53.77 N 20.48 E"
                     /collection_date="2021-04-20"
                     /collected_by="University of Warmia and Mazury in Olsztyn"
     gene            complement(join(3511..3868,1..359))
                     /locus_tag="V8955_RS00015"
                     /old_locus_tag="V8955_00015"
     CDS             complement(join(3511..3868,1..359))
                     /locus_tag="V8955_RS00015"
                     /old_locus_tag="V8955_00015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_064761962.1"
                     /translation="MWQARFYQSLPRIVADYPDARWMFLTLTVRNCAIGELGETLNRMN
                     MAFQRLKDRKEFRPVQGWIRTTEVTRGSDGSAHPHFHTLMMVPPSMFTRDYVKHDRWVE
                     LWRECLRVNYDPNVDVRAVKPRKPKDGESLASATAELVRGAVAETLKYSTKPADMVADP
                     EWFLELTKQTHKRRFVATGGALKDILKLDQETDADMVIGDDIPEGDDDGSRIAFEWKTE
                     SKKYRRSPSKDKAESD"
                     /name="protein rep"
     gene            complement(616..918)
                     /locus_tag="V8955_RS00020"
                     /old_locus_tag="V8955_00020"
     CDS             complement(616..918)
                     /locus_tag="V8955_RS00020"
                     /old_locus_tag="V8955_00020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023150476.1"
                     /translation="MAGSIRARSLYLYLRDNSGDKKWHQALRTKASGLICVVEGFGSIL
                     LGVVALKATPATTAIVRGCDHLQKFDSKRQPTGDTAMITRQMSHRPAWDGVHAKP"
                     /name="hypothetical protein"
     gene            complement(1577..2563)
                     /locus_tag="V8955_RS00025"
                     /old_locus_tag="V8955_00025"
     CDS             complement(1577..2563)
                     /locus_tag="V8955_RS00025"
                     /old_locus_tag="V8955_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_016245711.1"
                     /translation="MQNPKLQNLTDYSPSDAPWDAHKSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGETLNRMNMAFQRLKDRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPSMFTRDYVKHDRWVELWRECLRVNYDPNVDVRAVKPRKPKDGE
                     SLASATAELVRGAVAETLKYSTKPADMVADPEWFLELTKQTHKRRFVATGGALKDILKL
                     DQETDADMVIGDDIPEGDDDGSRIAFEWKTESKKYRRSPSKDKAESD"
                     /name="protein rep"
     gene            complement(2550..2852)
                     /locus_tag="V8955_RS00030"
                     /old_locus_tag="V8955_00030"
     CDS             complement(2550..2852)
                     /locus_tag="V8955_RS00030"
                     /old_locus_tag="V8955_00030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023150476.1"
                     /translation="MAGSIRARSLYLYLRDNSGDKKWHQALRTKASGLICVVEGFGSIL
                     LGVVALKATPATTAIVRGCDHLQKFDSKRQPTGDTAMITRQMSHRPAWDGVHAKP"
                     /name="hypothetical protein"
     Typing          1660..1851
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.601"
                     /coverage="100.0"
                     /evalue="1.39e-59"
     Typing          3594..3785
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.601"
                     /coverage="100.0"
                     /evalue="1.39e-59"
ORIGIN
        1 acatcaacat tcggatcgta attcacacgc aaacactctc gccaaagctc tacccagcgg
       61 tcatgtttaa cgtaatctcg cgtgaacatt gagggaggaa ccatcatcag agtgtgaaag
      121 tgcgggtgcg cactaccgtc acttccacgt gtaacttccg tggtgcggat ccagccctga
      181 acaggtcgaa actctttgcg atccttaagt cgctggaagg ccatgttcat tcggttaagt
      241 gtctcaccca attccccgat cgcgcagttt cgcactgtga gggtcaggaa catccagcgt
      301 gcatctggat aatccgcaac aatacgcggc agggactgat aaaaacgggc ttgccacatc
      361 agggatcgcc tccactggca gacaggacag tgccggacac ggcagaaatg cgcctcacgt
      421 agcctaaggc gagtttctcc agtttctttg agggtagacc aaccaaacct cagtaaaccg
      481 ccacaggaag ccatacgcgc cccgtagcgc tcatattcgg ctgcgagaag gtaaatccca
      541 cctacgtcgt cagaaacgct tttgtgagcg tcccagggcg cgtctgaggg ggagtaatcc
      601 gtgaggttct ggagtttagg gttttgcatg cacaccgtcc catgctgggc gatggctcat
      661 ttgacgtgtt atcattgccg tatcacctgt tggttgtcgc ttcgaatcga atttctgcag
      721 gtggtcacac ccccgaacga tggccgtcgt tgcgggggtc gcttttaagg cgaccactcc
      781 caacagaata cttccaaaac cttctacaac gcaaataagc cccgaagcct tggtacgcaa
      841 ggcttgatgc cattttttat cgccagagtt atcccttaag tataaataca aggatcgcgc
      901 gcggatcgaa ccagccattc aacgcccacc cgccaccaaa aactgccaca acgccaggag
      961 cgaaacagca acgctcaacg cactatcaac atgattaaga acctcaaaaa acataacaac
     1021 ctcactcgaa tggaattggg taaatctatt tagtctgtga tttgcgaacc catacttttt
     1081 cgcatcacga cagaaccaac tccattcgtt ctggttgtat ccggtaccag acaattaaaa
     1141 accgtcttcc aggtggttgg cctcacttcg tgaatccaac cacctcagcc aatttttcaa
     1201 tcagcaatgt cgtcaatcac taaatcggaa cccactggca agccagcggg accccacgat
     1261 ttagagtttg acgatttcgg aaacggcgag cgctggcaga ttttaatctg actgcgtgag
     1321 gtgttggcag ggtgtcgggg cgcagccctg acgaatcgtt tttggcaccg acatcgtgat
     1381 ggcggtgtac caaaaacaca tcttgtccca actgagttcg gaaaccgaac tcagtaattg
     1441 agtgacgtca ctttgcggcg ttagccctat cctgcatcgg agtagaacca ataggacaag
     1501 atgtgttttt ggagtaccgc cgacaagcgg cgccctctca aaaacgtcgt ggtcagggct
     1561 gcgccccgac accctgtcag tctgattcag ctttatcttt tgacggtgag cgacggtact
     1621 ttttggattc ggttttccac tcaaaagcaa tgcgtgaacc atcatcatcg ccttcgggaa
     1681 tatcatcccc gattaccata tcggcatcag tttcctgatc gagtttcagt atgtctttga
     1741 gtgcgccacc ggtagccaca aatcggcgct tatgtgtctg cttagtcaat tcgaggaacc
     1801 attcaggatc agcaaccatg tcagccggct tcgtggaata tttcagggtt tcagccactg
     1861 cgccccgtac aagttccgca gttgcagaag ccaatgattc tccgtcctta ggcttgcggg
     1921 gttttactgc tcgaacatca acattcggat cgtaattcac acgcaaacac tctcgccaaa
     1981 gctctaccca gcggtcatgt ttaacgtaat ctcgcgtgaa cattgaggga ggaaccatca
     2041 tcagagtgtg aaagtgcggg tgcgcactac cgtcacttcc acgtgtaact tccgtggtgc
     2101 ggatccagcc ctgaacaggt cgaaactctt tgcgatcctt aagtcgctgg aaggccatgt
     2161 tcattcggtt aagtgtctca cccaattccc cgatcgcgca gtttcgcact gtgagggtca
     2221 ggaacatcca gcgtgcatct ggataatccg caacaatacg cggcagggac tgataaaaac
     2281 gggcttgcca catcagggat cgcctccact ggcagacagg acagtgccgg acacggcaga
     2341 aatgcgcctc acgtagccta aggcgagttt ctccagtttc tttgagggta gaccaaccaa
     2401 acctcagtaa accgccacag gaagccatac gcgccccgta gcgctcatat tcggctgcga
     2461 gaaggtaaat cccacctacg tcgtcagaaa cgcttttgtg agcgtcccag ggcgcgtctg
     2521 agggggagta atccgtgagg ttctggagtt tagggttttg catgcacacc gtcccatgct
     2581 gggcgatggc tcatttgacg tgttatcatt gccgtatcac ctgttggttg tcgcttcgaa
     2641 tcgaatttct gcaggtggtc acacccccga acgatggccg tcgttgcggg ggtcgctttt
     2701 aaggcgacca ctcccaacag aatacttcca aaaccttcta caacgcaaat aagccccgaa
     2761 gccttggtac gcaaggcttg atgccatttt ttatcgccag agttatccct taagtataaa
     2821 tacaaggatc gcgcgcggat cgaaccagcc attcaacgcc cacccgccac caaaaactgc
     2881 cacaacgcca ggagcgaaac agcaacgctc aacgcactat caacatgatt aagaacctca
     2941 aaaaacataa caacctcact cgaatggaat tgggtaaatc tatttagtct gtgatttgcg
     3001 aacccatact ttttcgcatc acgacagaac caactccatt cgttctggtt gtatccggta
     3061 ccagacaatt aaaaaccgtc ttccaggtgg ttggcctcac ttcgtgaatc caaccacctc
     3121 agccaatttt tcaatcagca atgtcgtcaa tcactaaatc ggaacccact ggcaagccag
     3181 cgggacccca cgatttagag tttgacgatt tcggaaacgg cgagcgctgg cagattttaa
     3241 tctgactgcg tgaggtgttg gcagggtgtc ggggcgcagc cctgacgaat cgtttttggc
     3301 accgacatcg tgatggcggt gtaccaaaaa cacatcttgt cccaactgag ttcggaaacc
     3361 gaactcagta attgagtgac gtcactttgc ggcgttagcc ctatcctgca tcggagtaga
     3421 accaatagga caagatgtgt ttttggagta ccgccgacaa gcggcgccct ctcaaaaacg
     3481 tcgtggtcag ggctgcgccc cgacaccctg tcagtctgat tcagctttat cttttgacgg
     3541 tgagcgacgg tactttttgg attcggtttt ccactcaaaa gcaatgcgtg aaccatcatc
     3601 atcgccttcg ggaatatcat ccccgattac catatcggca tcagtttcct gatcgagttt
     3661 cagtatgtct ttgagtgcgc caccggtagc cacaaatcgg cgcttatgtg tctgcttagt
     3721 caattcgagg aaccattcag gatcagcaac catgtcagcc ggcttcgtgg aatatttcag
     3781 ggtttcagcc actgcgcccc gtacaagttc cgcagttgca gaagccaatg attctccgtc
     3841 cttaggcttg cggggtttta ctgctcga
//
