LOCUS       NZ_CP146558             1718 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain B1.H1.18 plasmid pB1.H1.18.c9, complete
            sequence.
ACCESSION   NZ_CP146558
VERSION     NZ_CP146558.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40199719
            Assembly: GCF_037081895.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Rolbiecki,D.
  TITLE     Genomic and metagenomic analysis reveals shared resistance genes and
            mobile genetic elements in E. coli and Klebsiella spp. isolated from
            hospital patients and hospital wastewater at intra- and inter-genus
            level
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Rolbiecki,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-FEB-2024) Department of Water Protection Engineering
            and Environmental Microbiology, Uniwersytet Warminsko-Mazurski w
            Olsztynie, Prawochenskiego 1, Olsztyn 10-720, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: GS De Novo Assembler v. Flye 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 392x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037081895.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 17:58:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,239
            CDSs (total)                      :: 5,120
            Genes (coding)                    :: 4,773
            CDSs (with protein)               :: 4,773
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 347
            CDSs (without protein)            :: 347
            Pseudo Genes (ambiguous residues) :: 0 of 347
            Pseudo Genes (frameshifted)       :: 199 of 347
            Pseudo Genes (incomplete)         :: 160 of 347
            Pseudo Genes (internal stop)      :: 35 of 347
            Pseudo Genes (multiple problems)  :: 44 of 347
            Pseudo Genes (short protein)      :: 4 of 347
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP146558.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B1.H1.18"
                     /isolation_source="Hospital Wastewater"
                     /db_xref="taxon:562"
                     /plasmid="pB1.H1.18.c9"
                     /geo_loc_name="Poland:Olsztyn"
                     /lat_lon="53.77 N 20.48 E"
                     /collection_date="2021-04-20"
                     /collected_by="University of Warmia and Mazury in Olsztyn"
     gene            complement(680..1084)
                     /locus_tag="V8955_RS00005"
                     /old_locus_tag="V8955_00005"
     CDS             complement(680..1084)
                     /locus_tag="V8955_RS00005"
                     /old_locus_tag="V8955_00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061604172.1"
                     /translation="MKILAAMEIADCDFEHMDMDVLVGALLKLKLWDSHHHNLNMQGKR
                     SFLDDLRADGIEAIKKYEAEIESENESFAEERLILRIYQNELGLWLGRLFCGEAEIGQC
                     GVYGSADEVRTAARQSGILCDSIEIFQKDA"
                     /name="hypothetical protein"
     gene            complement(1298..1534)
                     /locus_tag="V8955_RS00010"
                     /old_locus_tag="V8955_00010"
     CDS             complement(1298..1534)
                     /locus_tag="V8955_RS00010"
                     /old_locus_tag="V8955_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016230891.1"
                     /translation="MTRLTNLTPAEKKFIDDAIAAAERAAGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
ORIGIN
        1 accagtcacg tagcgctagc ggagtgtata ctggcttaat atgttggcat aggtgcggtt
       61 atcaggaaag tgcgtcatgt ggcaggtgtg atatgtggct cagaagcgtc cgcaatacca
      121 tgcccgaata gtgaaatgat gcctcctcgc tcactcggtc gctacgctcc ggacgtgaga
      181 ctgcggcggg cactacaact tttttcacat acatgaagtg aggtgtgacg gggatcttgt
      241 tgaaaaatca ggctacagat gtacaaaagc cccgtgacag aatcacaggg cttcggtact
      301 ctaattcttc gcagatacct gccaggatca gacgtagcaa tcctatccta gcagggctgg
      361 ctagccttgt cactaccagc ttaataccat tcaacgacat taaaatcata tctgcaccct
      421 tgaaactcag gtctacgccc cggactccgg caggataaac aggcaaacgg tggaaacgta
      481 acttagccgc gaaagagtta agctctaccc cgtctcgctg tacgataaca ggcacctgat
      541 aaagtgagat tcaaggcagg agttgcggtg aaatgcgatt gcccagtctg gcacaacacc
      601 cacacaatca gaacaacata gaacgacctg acgctggtcg ctccgctccc cgttttctgg
      661 tgtacccacc acgggggcat taggcatcct tttgaaaaat ttcgatgcta tcgcacaaaa
      721 taccgctttg acgcgcagct gttcgaacct catcagccga tccataaaca ccacactgac
      781 cgatctcagc ctcaccacaa aagagcctcc ctaaccataa gccaagctcg ttctgataga
      841 tcctcagtat taatcgctcc tccgcaaagg actcattctc agattcaatt tcagcctcgt
      901 atttcttaat agcctcaata ccatccgctc ttaaatcatc gagaaagctc ctcttacctt
      961 gcatgttcaa gttgtgatgg tggctatccc acaattttaa tttcagtaaa gcccctacta
     1021 agacatccat atccatatgt tcaaaatcac aatcagctat ttccattgca gccaaaatct
     1081 tcatacaccc tccgatttac cataacatcc gttatcagca ccccctgaca ggccctcagc
     1141 gcaattctga cgcatcagag ccgtttccgg cacatttcct cacccacatc ccggaaaaac
     1201 gcccctgatg atttctgcgc gactgcatag ctatgcacgc gggtgaaccg caggtgattt
     1261 cgcagcatca gcaccactaa accgccaaaa tccgcaatca gcggcgcggc gcgcgcggac
     1321 gtgaccaggc aaaatcggac tgctgccgct catcctcacg taatgcacgc tggcggtcgg
     1381 cgtaacgctg cgactcaatc tgcgcacgcg ctctgttcag gacgatgtgc cggttaggct
     1441 gattgagctt cttcccggcc gcacgttcgg cagcggctat agcgtcgtcg ataaactttt
     1501 tctcagcggg ggtcaggttt gtcagtctcg tcatgtttat ctccgtatga gaatagcccc
     1561 tgcagtctgc cagacacagt aggaacgatg aagcgtaagc gacggtgaaa ggcagtcaga
     1621 aacggtgaac gggagcggat tttaatccgg tgccgtgagg cgttggacaa gccgcaggcg
     1681 cgtcagtgga tttagcgggt ttcggggcgc agccctga
//
