LOCUS       NZ_CP146513             6818 bp    DNA     circular CON 06-APR-2024
DEFINITION  Escherichia coli strain MDP9-27 plasmid unnamed1, complete sequence.
ACCESSION   NZ_CP146513
VERSION     NZ_CP146513.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11997559
            Assembly: GCF_019585545.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   Gonzalez-Escalona,N., Hoffmann,M., Kalalah,A. and Eppinger,M.
  TITLE     STECs O113:H21
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6818)
  AUTHORS   Gonzalez-Escalona,N. and Hoffmann,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2024) ORS/DM/MMSB, Food and Drug
            Administration-College Park, 5001 Campus Drive, College Park, MD
            20740-3835, USA
REFERENCE   3  (bases 1 to 6818)
  AUTHORS   Allue-Guardia,A., Nyong,E.C., Koenig,S.K., Bosilevac,J.M., Feng,P.
            and Eppinger,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2019) Biology, University of Texas at San Antonio,
            One UTSA Circle, San Antonio, TX 78249, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Flye v. v2.9; Polypolish v. v0.5.0
            Assembly Name                     :: STEC2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80X
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019585545.2-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 22:54:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,996
            CDSs (total)                      :: 4,872
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 81 of 186
            Pseudo Genes (incomplete)         :: 103 of 186
            Pseudo Genes (internal stop)      :: 47 of 186
            Pseudo Genes (multiple problems)  :: 40 of 186
            Pseudo Genes (short protein)      :: 2 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP146513.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MDP9-27"
                     /serotype="O113:H21"
                     /isolation_source="Spinach"
                     /db_xref="taxon:562"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA"
                     /collection_date="2009"
     gene            complement(237..368)
                     /locus_tag="FIA76_RS00005"
                     /old_locus_tag="FIA76_000005"
     CDS             complement(237..368)
                     /locus_tag="FIA76_RS00005"
                     /old_locus_tag="FIA76_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            398..688
                     /gene="cki"
                     /locus_tag="FIA76_RS00010"
                     /old_locus_tag="FIA76_000010"
     CDS             398..688
                     /gene="cki"
                     /locus_tag="FIA76_RS00010"
                     /old_locus_tag="FIA76_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_071592231.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTRLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            complement(685..2157)
                     /locus_tag="FIA76_RS00015"
                     /old_locus_tag="FIA76_000015"
     CDS             complement(685..2157)
                     /locus_tag="FIA76_RS00015"
                     /old_locus_tag="FIA76_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421185.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_000362089.1"
                     /translation="MDKVTDNSPDVESTESTEGSFPTVGVDTGDTITATLATGTENVGG
                     GGGAFGGASESSAAIHATAKWSTAQLKKHQAEQAARAAAAEAALAKAKSQRDALTQRLK
                     DIVNDALRANAARSPSVTDLAHANNMAMQAEAERLRLAKAEQKAREEAEAAEKALREAE
                     RQRDEIARQQAETAHLLAMAEAAEAEKNRQDSLDEEHRAVEVAEKKLAEAKAELAKAES
                     DVQSKQAIVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLERQLQDKKNEYSSVTNALN
                     SAVSIRDAKKTDVQNAEIKLKEAKDALEKSQVKDSVDTMVGFYQYITEQYGEKYSRIAQ
                     DLAEKAKGSKFSSVDEALAAFEKYKNVLDKKISKVDRDAIFNALESVNYDELSKNLTKI
                     SKSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGV
                     PLGFWGIAIVTGIVSSYIGDDELNKLNELLGI"
                     /name="colicin-like pore-forming protein"
     gene            2402..2692
                     /locus_tag="FIA76_RS00020"
                     /old_locus_tag="FIA76_000020"
     CDS             2402..2692
                     /locus_tag="FIA76_RS00020"
                     /old_locus_tag="FIA76_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            complement(2684..2962)
                     /locus_tag="FIA76_RS00025"
                     /old_locus_tag="FIA76_000025"
     CDS             complement(2684..2962)
                     /locus_tag="FIA76_RS00025"
                     /old_locus_tag="FIA76_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            3084..3506
                     /locus_tag="FIA76_RS00030"
                     /old_locus_tag="FIA76_000030"
     CDS             3084..3506
                     /locus_tag="FIA76_RS00030"
                     /old_locus_tag="FIA76_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_021539380.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTT
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(3990..4223)
                     /locus_tag="FIA76_RS00035"
                     /old_locus_tag="FIA76_000035"
     CDS             complement(3990..4223)
                     /locus_tag="FIA76_RS00035"
                     /old_locus_tag="FIA76_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4230..4790)
                     /locus_tag="FIA76_RS00040"
                     /old_locus_tag="FIA76_000040"
     CDS             complement(4230..4790)
                     /locus_tag="FIA76_RS00040"
                     /old_locus_tag="FIA76_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_074167501.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4825..5505)
                     /locus_tag="FIA76_RS00045"
                     /old_locus_tag="FIA76_000045"
                     /pseudo=""
     CDS             complement(<4825..5505)
                     /locus_tag="FIA76_RS00045"
                     /old_locus_tag="FIA76_000045"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5468..5791)
                     /locus_tag="FIA76_RS00050"
                     /old_locus_tag="FIA76_000050"
     CDS             complement(5468..5791)
                     /locus_tag="FIA76_RS00050"
                     /old_locus_tag="FIA76_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5859..6050
                     /locus_tag="FIA76_RS00055"
                     /old_locus_tag="FIA76_000055"
     CDS             5859..6050
                     /locus_tag="FIA76_RS00055"
                     /old_locus_tag="FIA76_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(6136..6215)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.765"
                     /coverage="100.0"
                     /evalue="7.760000000000001e-33"
     Typing          6234..6792
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.179"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3884..5478)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.436"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5851..6053)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.176"
                     /coverage="100.0"
                     /evalue="5.43e-12"
ORIGIN
        1 attggtaact ggatagtgga tttagatacg cagaactctt gaagttgaag ccttatagcg
       61 gctacactgg aaggacagca tttggtatct gtgctccact aaagccagtt acccggttaa
      121 gcagtcccca actgacttaa ccttcgacta aaccgcctcc ccaggcggtt ttttcgttta
      181 caggcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
      241 tgaaccgcga tccccgtcag ttcagaagac gaagatggtg caacggttcc tccttgcaca
      301 tcacgaatat agtttgcttg acatcctgac aagaataata ttgtaattag aaaaattatt
      361 attttcatat ttctttccaa caaaagtaaa aatacttatg catttaaaat actacctaca
      421 taatttacct gaatcactta taccatggat tcttatttta atatttaacg acaatgataa
      481 cactcctttg ttatttatat ttatatcatc aatacatgta ttgctatatc catactctaa
      541 attaaccata tctagatata tcaaagaaaa tacaaggtta aaaaaagaac cctggtactt
      601 atgcaagtta tctgcattgt tttatttatt aatggcaatc ccagtaggat tgccaagttt
      661 catatattac actctaaaga gaaattaaat ccctaacaac tcattaagtt tgttcaactc
      721 atcatcccct atgtaagaag aaacaatacc tgtgacaatt gcaatccccc agaaaccaag
      781 aggcacacca acaataaaac taaacattaa tgccacaatt tttgccacac ctacatcgac
      841 cgcacttttc tccagcgtga caaacaaagg tcgccagtta ccagtttcta ttgcattttt
      901 aaaatcagaa ccaacatcat atagaaaaga tactcgacta gtgattttaa gcgactttga
      961 tatcttcgtt aaattcttag ataactcatc ataattaaca gactctaacg cattaaaaat
     1021 agcatcccga tcaaccttac tgattttctt atccagtaca tttttatact tttcaaatgc
     1081 agcaagagct tcatcaacac tactaaattt actacccttc gccttttcag ctaaatcctg
     1141 agctattctg gaatattttt ccccatattg ttcggttata tattgataaa acccaaccat
     1201 agtatcaaca gagtctttta cctgactctt ctcaagagca tccttagctt cttttaattt
     1261 tatctcagca ttctgaacat ctgttttttt agcatctcta atgctaacag cagaattaag
     1321 agcattcgtc actgacgaat attcattctt cttatcctgc aattgtctct ccagtttttt
     1381 ctgaacatca cgccatccag gaaatccagt aaccttcaca tcaacacttt tttgagcgtt
     1441 ttcaagctcc cctgcaactc tggaaacaat cgcttgctta ctctgtacat cgctttcggc
     1501 cttcgccagt tcagctttag cctcagccag cttcttctct gccacttcca cagcccgatg
     1561 ctcttcatca agagaatcct gtcgattttt ctcagcctct gctgcctccg ccattgctaa
     1621 caaatgcgcg gtttcagcct gttggcgggc aatctcatca cgttggcgtt ctgcttcccg
     1681 gagcgctttt tctgctgctt cagcttcttc acgggctttt tgctctgcct tcgcaaggcg
     1741 caaacgctca gcctctgcct gcattgccat attattggca tgagcaaggt cagttactga
     1801 tggactacga gcggcattag cacgtaaagc gtcattaaca atatccttga gacgttgagt
     1861 cagggcatca cgctgagatt tcgcttttgc caatgctgcc tcagccgcag cagcacgggc
     1921 agcctgttca gcctgatgtt ttttcaactg cgcggtagac catttagcgg ttgcatgtat
     1981 cgcagcagaa ctttcactgg ccccaccaaa tgctccaccg cctccaccaa cattttcagt
     2041 tccagttgca agcgtcgctg taatcgtatc gccagtatca accccaacag ttgggaatga
     2101 cccctcagta gattctgtgc tctccacatc tggagaatta tcagtgactt tatccataaa
     2161 gaaattcctc tttgataatt aaaacaataa attaaaaaca acatactgta catataacca
     2221 ctggttttat gtacagtgaa aacctactac tcagcattgt ccatgtcaag agcatggatt
     2281 ttcatttttg caataaggat cacactatgg ggaggcaggc attgagaacg tcgaaacaga
     2341 acaccggagc aaatcaggat gagatataaa actgttggat catgaaaaaa cggagaacga
     2401 tgtgagcaaa tcaccccgcc ataaactgaa caaaacagac aaacgacttc tcgacaccct
     2461 tgttgctgcc ggatatgagc atgacaaagc ccgtgacctc atccagaaac aggtttacac
     2521 gctgacactg gcagatcagc gtcatgtggt cagtgaaatc agtaatggtg tgaatcccac
     2581 ccaggcttac tcggcggtat accaggcaag acgcattcgc ctcgcccgta aatatctgaa
     2641 cggtaaaaag gttatggaag aaaccgggga aaatacgccc ccatcagcgt aaggatttct
     2701 tttgccgctc cagagactcc agttttttac gcaaatcctc tcttttttgg gcatctctgg
     2761 tgccagccag ttctgctctc aactcatcga tctgaagttg tatcttcagt ctgttactga
     2821 acattttctg tctggcatta acatccgcaa caatgccgtt ttttgtcttc tcggcctttt
     2881 gttgaaaaac attgctgtcc gcatgactgg caaccgaagc agaaagaaca ctaaaaagca
     2941 ggactggcac acattttttc acgggattat tcctgactca ttgaccatca aatcacattg
     3001 ggagtaaacc gacgtatgat aagagatact cttcggagat ataactccct gagtatcaag
     3061 attaaaaacg caaggagatg tttatgagat ctgccgctgc caggctgctt ctgatacctc
     3121 tgataacagc aacaatagct cttacaggat gcacaccaaa gaccagcctg gaacgacata
     3181 cccggcatta tgtttatgct tcagatgatg gatttgatcc taacttctac acccagaaag
     3241 cagacaccat acgtatgatg ctcccgttct ttcagcagtt ccgggatatg gggatgaaag
     3301 acaaagcagc cggagtatca gcagaaacgg cacagcaacg tgtaaaagaa ttccactcag
     3361 aaaaattttt tcactcactc cggagcacaa caacctttgc tggcagaaaa tacacaaaca
     3421 gcgatatgcc ttcgccgaaa aaaatgaaac taatggcaga caccatttct gcggtttatc
     3481 tcgatggata cgagggcaga cagtaaggga tttaccataa tcccttaatt gtacgcaccg
     3541 ctgaaatgcg ttcagcgcga tcacggctgc tgacaggtaa aaatggcaac aaaccacccg
     3601 aaaagctgcc gcgatcgcac ctgataaatt ttaaccgtat gcatagctat tcagccatgt
     3661 gaataacgct ggttttgcct gcgtaaacct catgacactg ttttttttcc atcttttcag
     3721 ttgatgacat acgcagacat cgcaggatga ggctgaggaa tgagcgcgat ctggcaaaga
     3781 ggcaaaacac agcaacaaaa acgacacgcc agaatcgcgc ccggatgcgt ttttaacgcg
     3841 ttccggtacc atctggcaac ctcccggaac aactcaccgt cacatcccca ttgacgggcc
     3901 acgccatacc tgtgcttccc gttcctgctc ttcatgccag gaccgcgcac gctcccgttc
     3961 caggcgtgcc tgcctttcct gttcatccct tatctgctgt tcgtgataaa taaccgactc
     4021 aagcggtcca cctgcccggc taatctctgc acctgctgac tcaagtcgtc gcactgttcc
     4081 ctcagttgcc cgttctcctg tcgtgtcagc tcgaacatat gctgcaaatc cgtgaaggcg
     4141 ctctcccagt ctttcagccg ctgcgtatag tcctgctgca attgctctaa ggcgttcagt
     4201 aagtgcattt ccagctctgt catactcact tactccctga ccagtcttac tgcgttcttc
     4261 ttctccaccg tccagttgtt ttcccccttc accccggacg gcaacaccag aaatttcccg
     4321 ttcctgccct cgtgatacgt cacaccccat gttttttccc ggagtttcgc cagcgtctct
     4381 tcctggtccc tgatagccag gatgttcgcc gcaatccggc tttcctgcca ctgaatcagc
     4441 ccccatgacg ccagaaaaat cccgcccgtg acgcaaagcg ccgtcagcga cgggtacagt
     4501 atccgccctt tgatcagctt ccagagcagc tcttcctgcc gccgggccag ttcgttctct
     4561 gtggcgctga actgcgcgtt cacggcactg ttcagcgtct ccagttgttc tttcaccgct
     4621 gctgtgtgtg cgctgatagc gtctctgatt ttctgcccgt ttaagttcag ttccctgtct
     4681 acagacgctt cgagcttcct gaactcgctg ttcagcatgt tctctgtaga gacggcacgc
     4741 tctttcagtt tcttctcgaa gtctgtcccc atttgtaaaa gattgctcat acagcgcccc
     4801 tttcagcctg agattacgcc cgccctccgg gtcggcgata ctgatactgc tcctggttgt
     4861 cctcaccacc tcaaaacctg ccgctgtaag cgcctcagtg acatcctgac gcgtttttag
     4921 cgctccggca tggtaaagag cctccagtcc cctcgtaatc gcttctgcgg cctcctgttt
     4981 cgctttcggc agattattcg gggtgacaag tgtccgcctg ttctccggtg cgttcgggtc
     5041 gtgcagcccg taatggtgat tcaccagtgt ctgccaggca ttgattcgcg gacggtccgc
     5101 tctgtcgtaa tagggctgga gccgttttcc gctcgccagc tccatattcg ggatgacaaa
     5161 attcagctca agacgcccct tgtcctggtg ctccacccac aggatgctgt actgattttt
     5221 ttcaagaccg ggcatcagta cccgctcaaa gctctccatc accctttcac gctctcccgg
     5281 tggcagggtc tgctctgcaa aagacagaac ccccgaggtg tattttttcg caaacggcgt
     5341 ggcatcgatg agttcccgta cctcttcggg agcaccccgc agaactctcg ccccttcccg
     5401 gttacgctcc cggcccagca ggtaatcaac cggaccactg ccaccgcctt ttcccctggc
     5461 atgaaactta actatcatcc cgttctccct gtttacggac ctcatccctc agctcactca
     5521 gttcacgccc gatggccatc agtgcagcca ccacatgaac ccggtcatgc cccgaccact
     5581 gtccgctgtt tatcttccgg gctatctgat tcaggttatt gccgaccgaa gcaaactggc
     5641 gcaacagcgg cggtgccagt gtcggaaggc ctgacgtttt cgatggcggt gcccccaggc
     5701 agaccttacg catccatgac gcaagttgtt ttccctcaca acgtgccagc agccgcgcat
     5761 gttcctcatc cgtgacccgt atcgtgagca ttctttcgcg tttcaccggt atcattaaaa
     5821 acctccgaca gactccccac acatggagaa acagaactgt gactaaacag gaaaaaaccg
     5881 cccttaacat ggcccgcttc atcagaagcc agacgctgac cctgctggaa aaactgaatg
     5941 aactggacgc cgacgaccaa gctgacatct gcgaagcgct tcacgaccac gccgacgagc
     6001 tttaccgcag ctgcctcgca cgcttcgggg atgacggtga aaacctctga cacatgcagc
     6061 tcccggagac gggcacagct tacctgtgag cggattccgg gagcagacaa gcccgtcagg
     6121 gcgcgtcagc aggttttagc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     6181 gcggagtgta tactggctta cttattcggc atcagggcgc attgtatgaa aagtgcacca
     6241 tgccgggtgt gaaatgccgc acagatgcat aaggagaaaa tgctcgtccc ggcgcttttc
     6301 cgcttcctcg ctcactgact cgctacgctc ggccgttcga ctgcggcgag cggtgtctgc
     6361 tcacgcaaag acggagatac agttatccac agaatcaggg gataaggccg gaaagaacat
     6421 gtgagcaaaa gaccaggaac aggaagaagg ccgcgccgct ggcgtttttc cataggctcc
     6481 gcccccctga cgagcatcac aaaaacagac gctcaagtca gaggtggcga aacccgacag
     6541 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6601 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaaacgtg gcgctttctc
     6661 atagctcacg ctgttggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6721 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6781 ccaacccggt aagacacgac ttaacgccac tggcagca
//
