LOCUS       NZ_CP146510             7769 bp    DNA     circular CON 06-APR-2024
DEFINITION  Escherichia coli strain 10 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP146510
VERSION     NZ_CP146510.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11997563
            Assembly: GCF_019585465.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Gonzalez-Escalona,N., Hoffmann,M., Kalalah,A. and Eppinger,M.
  TITLE     STECs O113:H21
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7769)
  AUTHORS   Gonzalez-Escalona,N. and Hoffmann,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2024) ORS/DM/MMSB, Food and Drug
            Administration-College Park, 5001 Campus Drive, College Park, MD
            20740-3835, USA
REFERENCE   3  (bases 1 to 7769)
  AUTHORS   Allue-Guardia,A., Nyong,E.C., Koenig,S.K., Bosilevac,J.M., Feng,P.
            and Eppinger,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2019) Biology, University of Texas at San Antonio,
            One UTSA Circle, San Antonio, TX 78249, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Assembly Name                     :: STEC10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 90X
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019585465.2-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 22:49:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,078
            CDSs (total)                      :: 4,956
            Genes (coding)                    :: 4,774
            CDSs (with protein)               :: 4,774
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 81 of 182
            Pseudo Genes (incomplete)         :: 97 of 182
            Pseudo Genes (internal stop)      :: 40 of 182
            Pseudo Genes (multiple problems)  :: 34 of 182
            Pseudo Genes (short protein)      :: 2 of 182
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP146510.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="10"
                     /serotype="O113:H21"
                     /isolation_source="Ground beef"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA"
                     /collection_date="2006"
     gene            complement(<71..541)
                     /locus_tag="FIA71_RS25320"
                     /old_locus_tag="FIA71_025320"
                     /pseudo=""
     CDS             complement(<71..541)
                     /locus_tag="FIA71_RS25320"
                     /old_locus_tag="FIA71_025320"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(504..827)
                     /locus_tag="FIA71_RS25325"
                     /old_locus_tag="FIA71_025325"
     CDS             complement(504..827)
                     /locus_tag="FIA71_RS25325"
                     /old_locus_tag="FIA71_025325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            895..1086
                     /locus_tag="FIA71_RS25330"
                     /old_locus_tag="FIA71_025330"
     CDS             895..1086
                     /locus_tag="FIA71_RS25330"
                     /old_locus_tag="FIA71_025330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1757..1900
                     /locus_tag="FIA71_RS25335"
                     /old_locus_tag="FIA71_025335"
     CDS             1757..1900
                     /locus_tag="FIA71_RS25335"
                     /old_locus_tag="FIA71_025335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_152896351.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     gene            complement(2095..2226)
                     /locus_tag="FIA71_RS25340"
                     /old_locus_tag="FIA71_025340"
     CDS             complement(2095..2226)
                     /locus_tag="FIA71_RS25340"
                     /old_locus_tag="FIA71_025340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            2256..2546
                     /gene="cki"
                     /locus_tag="FIA71_RS25345"
                     /old_locus_tag="FIA71_025345"
     CDS             2256..2546
                     /gene="cki"
                     /locus_tag="FIA71_RS25345"
                     /old_locus_tag="FIA71_025345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            complement(2543..4015)
                     /locus_tag="FIA71_RS25350"
                     /old_locus_tag="FIA71_025350"
     CDS             complement(2543..4015)
                     /locus_tag="FIA71_RS25350"
                     /old_locus_tag="FIA71_025350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421185.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_000362089.1"
                     /translation="MDKVTDNSPDVESTESTEGSFPTVGVDTGDTITATLATGTENVGG
                     GGGAFGGASESSAAIHATAKWSTAQLKKHQAEQAARAAAAEAALAKAKSQRDALTQRLK
                     DIVNDALRANAARSPSVTDLAHANNMAMQAEAERLRLAKAEQKAREEAEAAEKALREAE
                     RQRDEIARQQAETAHLLAMAEAAEAEKNRQDSLDEEHRAVEVAEKKLAEAKAELAKAES
                     DVQSKQAIVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLERQLQDKKNEYSSVTNALN
                     SAVSIRDAKKTDVQNAEIKLKEAKDALEKSQVKDSVDTMVGFYQYITEQYGEKYSRIAQ
                     DLAEKAKGSKFSSVDEALAAFEKYKNVLDKKISKVDRDAIFNALESVNYDELSKNLTKI
                     SKSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGV
                     PLGFWGIAIVTGIVSSYIGDDELNKLNELLGI"
                     /name="colicin-like pore-forming protein"
     gene            4260..4550
                     /locus_tag="FIA71_RS25355"
                     /old_locus_tag="FIA71_025355"
     CDS             4260..4550
                     /locus_tag="FIA71_RS25355"
                     /old_locus_tag="FIA71_025355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024200900.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGMNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            complement(4542..4820)
                     /locus_tag="FIA71_RS25360"
                     /old_locus_tag="FIA71_025360"
     CDS             complement(4542..4820)
                     /locus_tag="FIA71_RS25360"
                     /old_locus_tag="FIA71_025360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            4942..5364
                     /locus_tag="FIA71_RS25365"
                     /old_locus_tag="FIA71_025365"
     CDS             4942..5364
                     /locus_tag="FIA71_RS25365"
                     /old_locus_tag="FIA71_025365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_032319274.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLQSTTT
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(5423..5689)
                     /locus_tag="FIA71_RS25370"
                     /old_locus_tag="FIA71_025370"
     CDS             complement(5423..5689)
                     /locus_tag="FIA71_RS25370"
                     /old_locus_tag="FIA71_025370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000262325.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRKSGR
                     PLKGELGEFWRYRVGDYRILCEIRDDELIILVATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5673..5897)
                     /locus_tag="FIA71_RS25375"
                     /old_locus_tag="FIA71_025375"
     CDS             complement(5673..5897)
                     /locus_tag="FIA71_RS25375"
                     /old_locus_tag="FIA71_025375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879770.1"
                     /translation="MLAIRLSDEIESRLDSLAKKTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            6051..6263
                     /locus_tag="FIA71_RS25380"
                     /old_locus_tag="FIA71_025380"
     CDS             6051..6263
                     /locus_tag="FIA71_RS25380"
                     /old_locus_tag="FIA71_025380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000350688.1"
                     /translation="MDIEMNNTEEMFSKLGMRLGAEMAIPLKDGDNFAPTGCIVTKLDK
                     ENGLLICRPVQKEINGTWYTVDLSK"
                     /name="hypothetical protein"
     gene            complement(6795..7028)
                     /locus_tag="FIA71_RS25385"
                     /old_locus_tag="FIA71_025385"
     CDS             complement(6795..7028)
                     /locus_tag="FIA71_RS25385"
                     /old_locus_tag="FIA71_025385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(7035..7595)
                     /locus_tag="FIA71_RS25390"
                     /old_locus_tag="FIA71_025390"
     CDS             complement(7035..7595)
                     /locus_tag="FIA71_RS25390"
                     /old_locus_tag="FIA71_025390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001345151.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTVGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     Typing          complement(1172..1259)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          1291..1829
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3..514)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.415"
                     /coverage="99.0"
                     /evalue="7.77e-107"
     Typing          complement(887..1089)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.706"
                     /coverage="100.0"
                     /evalue="3.88e-12"
     Typing          complement(6689..7767)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.444"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ctgcggcctc ctgtttcgct ttcggcagat tattcggggt gacaagtgtc cgcctgttct
       61 ccggtgcgtt cgggtcgtgc agcccgtaat ggtgattcac cagtgtctgc caggcattga
      121 ttcgcggacg gtccgcccgg tcgtaatagg gctggagccg ttttccgctc gccagctcca
      181 tattcgggat gacaaaattc agctcaagac gccccttgtc ctggtgctcc acccacagga
      241 tgctgtactg atttttttca agaccgggca tcagtacccg ctcaaagctc tccatcaccc
      301 tttcacgctc tcccggtggc agtgtctgct ctgcaaaaga cagaaccccc gaggtgtatt
      361 ttttcgcaaa cggcgtggca tcgatgagtt cccgcacctc ttcgggagca ccccgcagaa
      421 ctctcgcccc ttcccggtta cgctcccggc ccagcaggta atcaaccgga ccactgccac
      481 cgccttttcc cctggcatga aacttaacta tcatcgcgtt ctccctgttt acggacctca
      541 tccctcagct cactcagttc acgtccgatg gccatcagtg cagccaccac atgaacccgg
      601 tcatgccccg accactgtcc actgtttatc ttccgggcta tctgattcag gttattgccg
      661 accgaagcga actggcgcaa cagcggcggt gccagtgtcg gaagacctga cgttttcgat
      721 ggcggtgccc ccaggcagac cttacgcatc catgacgcaa gttgttttcc ctcacaacgt
      781 gccagcagcc gcgcatgttc ctcatccgtg acccgtatcg tgagcatcct ttcgcgtttc
      841 accggtatca ttaaaaacct ccgacagact ccccacacat ggagaaacag aactgtgact
      901 aaacaggaaa aaaccgccct taacatggcc cgcttcatca gaagccagac gctgaccctg
      961 ctggaaaaac tgaatgaact ggacgccgac gaccaggctg acatctgcga agcgcttcac
     1021 gaccacgctg acgagcttta ccgcagctgc ctcgcacgct tcggggatga cggtgaaaac
     1081 ctctgacaca tgcagctccc ggagacggtc acagcttgcc tgtgagcgga tgccgggagc
     1141 agacaagccc gtcagggcgc gtcagcgggt tttagcgggt gtcggggcgc agccatgacc
     1201 cagtcacgta gcgatagcgg agtgtatact ggcttaatta tgcggcaaca gtgcggattg
     1261 tatgaaaagt gcgccatgcc gggtgtgaaa tgccgcacag atgcgtaagg agagaatgca
     1321 cgtccaggcg cttttccgct tcctcgctca ctgactcgct ccgctcggtc gttcggctgc
     1381 ggcgagcgga ctctcctttc tcaaaggcgg taaccccgtt atccacagaa tccggggata
     1441 acccgggaaa gaacatgtgc gcaaaaaaac gaagaccccg ggaaaggccg cggcacaggc
     1501 gctttttcca taggctccgc ccccctgacg agcatcacaa aaaccgacgc tcaagtcaga
     1561 ggtggcgaaa cccgacagga cttaaagata ccaggcgttt ccccccggaa gctccctcgt
     1621 gcgctctcct gttccgaccc tgccgattac cggatacctg tccgcctttc tcccttcggg
     1681 aagcgtggcg ctttctcata gctcacgctg ttggtatctc agttcggtgt aggtcgttcg
     1741 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1801 taactatcgt cttgagtcca acccggtaag acacgactta acgccactgg cagcagccat
     1861 tggtaactgg atagtggatt tagatacgca gaactcttga agttgaagcc ttatagcggc
     1921 tacactggaa ggacagcatt tggtatctgt gctccactaa agccagttac ccggttaagc
     1981 agtccccaac tgacttaacc ttcgactaaa ccgcctcccc aggcggtttt ttcgtttaca
     2041 ggcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctactg
     2101 aaccgcgatc cccgtcagtt cagaagacga agatggtgca acggttcctc cttgcacatc
     2161 acgaatatag tttgcttgac atcctgacaa gaataatatt gtaattagaa aaattattat
     2221 tttcatattt ctttccaaca aaagtaaaaa tacttatgca tttaaaatac tacctacata
     2281 atttacctga atcacttata ccatggattc ttattttaat atttaacgac aatgataaca
     2341 ctcctttgtt atttatattt atatcatcaa tacatgtatt gctatatcca tactctaaat
     2401 taaccatatc tagatatatc aaagaaaata caaagttaaa aaaagaaccc tggtacttat
     2461 gcaagttatc tgcattgttt tatttattaa tggcaatccc agtaggattg ccaagtttca
     2521 tatattacac tctaaagaga aattaaatcc ctaacaactc attaagtttg ttcaactcat
     2581 catcccctat gtaagaagaa acaatacctg tgacaattgc aatcccccag aaaccaagag
     2641 gcacaccaac aataaaacta aacattaatg ccacaatttt tgccacacct acatcgaccg
     2701 cacttttctc cagcgtgaca aacaaaggtc gccagttacc agtttctatt gcatttttaa
     2761 aatcagaacc aacatcatat agaaaagata ctcgactagt gattttaagc gactttgata
     2821 tcttcgttaa attcttagat aactcatcat aattaacaga ctctaacgca ttaaaaatag
     2881 catcccgatc aaccttactg attttcttat ccagtacatt tttatacttt tcaaatgcag
     2941 caagagcttc atcaacacta ctaaatttac tacccttcgc cttttcagct aaatcctgag
     3001 ctattctgga atatttttcc ccatattgtt cggttatata ttgataaaac ccaaccatag
     3061 tatcaacaga gtcttttacc tgactcttct caagagcatc cttagcttct tttaatttta
     3121 tctcagcatt ctgaacatct gtttttttag catctctaat gctaaccgca gaattaagag
     3181 cattcgtcac tgacgaatat tcattcttct tatcctgcaa ttgtctctcc agttttttct
     3241 gaacatcacg ccatccagga aatccagtaa ccttcacatc aacacttttt tgagcgtttt
     3301 caagctcccc tgcaactctg gaaacaatcg cttgcttact ctgtacatcg ctttcggcct
     3361 tcgccagttc agctttagcc tcagccagct tcttctctgc cacttccaca gcccgatgct
     3421 cttcatcaag agaatcctgt cgatttttct cagcctctgc tgcctccgcc attgctaaca
     3481 aatgcgcggt ttcagcctgt tggcgggcaa tctcatcacg ttggcgttct gcttcccgga
     3541 gcgctttttc tgctgcttca gcttcttcac gggctttttg ctctgccttc gcaaggcgca
     3601 aacgctcagc ctctgcctgc attgccatat tattggcatg agcaaggtca gttactgatg
     3661 gactacgggc ggcattagca cgtaaagcgt cattaacaat atccttgaga cgttgagtca
     3721 gggcatcacg ctgagatttc gcttttgcca atgctgcctc agccgcagca gcacgggcag
     3781 cctgttcagc ctgatgtttt ttcaactgcg cggtagacca tttagcggtt gcatgtatcg
     3841 cagcagaact ttcactggcc ccaccaaatg ctccaccgcc tccaccaaca ttttcagttc
     3901 cagttgcaag cgtcgctgta atcgtatcgc cagtatcaac cccaacagtt gggaatgacc
     3961 cctcagtaga ttctgtgctc tccacatctg gagaattatc agtgacttta tccataaaga
     4021 aattcctctt tgataattaa aacaataaat taaaaacaat atactgtaca tataaccact
     4081 ggttttatgt acagtgaaaa cctactactc agcattgtcc atgtcaagag catggatttt
     4141 catttttgca ataaggatca cactatgggg aggcaggcat tgagaacgtc gaaacagaac
     4201 accggagcaa atcaggatga gatataaaac tgttggatca tgaaaaaacg gagaacgatg
     4261 tgagcaaatc accccgccat aaactgaaca aaacagacaa acgacttctc gacacccttg
     4321 ttgctgccgg atatgagcat gacaaagccc gtgacctcat ccagaaacag gtttacacgc
     4381 tgacactggc agatcagcgt catgtggtca gtgaaatcag taatggtatg aatcccaccc
     4441 aggcttactc ggcggtatac caggcaagac gcattcgcct cgcccgtaaa tatctgaacg
     4501 gtaaaaaggt tatggaagaa accggggaaa atacgccccc atcagcgtaa ggatttcttt
     4561 tgccgctcca gagactccag ttttttacgc aaatcctctc ttttttgggc atctctggtg
     4621 ccagccagct ctgctctcaa ctcatcgatc tgaagttgta tcttcagtct gttactgaac
     4681 attttctgtc tggcattaac atccgcaaca atgccgtttt ttgtcttctc ggccttttgt
     4741 tgaaaaacat tgctgtccgc atgactggca accgaagcag aaagaacact aaaaagcagg
     4801 actggcacac attttttcac gggattattc ctgactcatt gaccatcaaa tcacattggg
     4861 agtaaaccga cgtatgataa gagatactct tcggagatat aactccctga gtatcaagat
     4921 taaaaacgca aggagatgtt tatgagatct gccgctgcca ggctgcttct gatacctctg
     4981 ataacagcaa caatagctct tacaggatgc acaccaaaga ccagcctgga acgacatacc
     5041 cggcattatg tttatgcttc agatgatgga tttgatccta acttctacac ccagaaagca
     5101 gacaccatac gtatgatgct cccgttcttt cagcagttcc gggatatggg gatgaaagac
     5161 aaagcagccg gagtatcagc agaaacggca cagcaacgtg taaaagaatt ccactcagaa
     5221 aaattttttc actcactcca gagcacaaca acctttgctg gcagaaaata cacaaacagc
     5281 gatatgcctt cgccgaaaaa aatgaaacta atggcagaca ccatttctgc ggtttatctc
     5341 gatggatacg agggcagaca gtaagggatt tatcataatc ccttattgta cgccacatat
     5401 gaaaaagccc gcatggcggg cttcagtcgt agacttcacg tcgatggccg atggtggcca
     5461 caagaataat cagttcgtca tccctgattt cacacagaat gcgataatca cccacacgat
     5521 aacgccagaa ttctcccaac tcccccttca gtggcctccc tgatttacgg ggatcggctg
     5581 caactgcaat acgtaaatcc ataaaatcca caatccgtcg cgcgttctgc ttgtccatct
     5641 tacgtagcga tttgagcgcc cgatcggaat aattaatcgt ccagaccaag agacttcctt
     5701 acgtcctcag acgaatgcac agcctcatca ccacggcgaa cacgtgctgc tgtttccgct
     5761 gaaagataat aatcttccag atcttccagg tgcgccagta tcgcttcccg ggcatagaac
     5821 gtctttgtcc tcccggtttt cttcgccagc gagtccagac gggactcaat ttcatcagac
     5881 aaccgaatag ccagcataca aatctccctc cgaacaattg ttatacatgt atagcaaaaa
     5941 ttataacgta aaaagacgat aaaacaatca aaggctggtt gaataaacag taaaatccca
     6001 tatattaacg actgatatat gataagtatc ggttttaaaa ggaaaacaac atggatattg
     6061 aaatgaataa cactgaagag atgttcagca aactggggat gcggttagga gctgagatgg
     6121 caatcccttt gaaggatggt gataattttg cgccaactgg atgcatcgtt acgaagcttg
     6181 ataaagagaa tggccttctg atctgccgac cagttcagaa ggaaatcaac ggcacgtggt
     6241 acaccgtcga tttaagtaag taacaccacg gactaaagcc ctgcggggct tttttatacc
     6301 tgccgctgac tctgattgaa cataacatct gaattgtacg caccgctgaa atgcgttcag
     6361 cgcgatcacg gctgctgaca ggtaaaaatg gcaacaaacc acccgaaaag ctgccgcgat
     6421 cgcacctgat aaattttaac cgtatgcata gctattcagc catgtgaata acgctggttt
     6481 tgcctgcgta aacctcatga cactgttttt tttccatctt ttcagttgat gacatacgca
     6541 gacatcgcgg gatgaggctg aggaatgagc gcgatctggc aaagaggcaa aacacagcaa
     6601 caaaaacgac acgccagaat cgcgcccgga tgcgttttta acgcgttccg gtaccatctg
     6661 gcaacctccc ggaacaactc accgtcacat ccccattgac gggccacgcc atacccgtgc
     6721 ttcccgttcc tgctcttcat gccaggaccg cgcacgctcc cgttccaggc gtgcctgcct
     6781 ttcctgttca tcccttatct gctgttcgtg ataaataacc gactcaagcg gtccacctgc
     6841 ccggctaatc tctgcacctg ctgactcaag tcgtcgcact gttccctcag ttgcccgttc
     6901 tcctgtcgtg tcagctcgaa catatgctgc aaatccgtga aggcgctctc ccagtctttc
     6961 agccgctgcg tatagtcctg ctgcaattgc tctaaggcgt tcagtaagtg catttccagc
     7021 tctgtcatac tcacttactc cctgaccagt cttactgcgt tcttcttctc caccgtccag
     7081 ttgttttccc ccttcacccc ggacggcaac accagaaatt tcccgttcct gccctcgtga
     7141 tacgtcacac cccatgtttt ttcccggagt ttcgccagcg tctcttcctg gtccctgata
     7201 gccaggatgt tcgccgcaat acggctttcc tgccactgaa tcagccccca tgacgccaga
     7261 aaaatcccga ccgtgacgca aagcgccgtc agcgacgggt acagtatccg ccctttgacc
     7321 agcttccaga gcagctcttc ctgccgccgg gcaagttcgt tctctgtggc gctgaactgc
     7381 gcgttcacgg cactgttcag cgtctccagt tgttctttca ccgctgctgt gtgtgcgctg
     7441 atagcgtctc tgattttctg cccgtttaag ttcagttccc tgtctacaga cgcttcgagc
     7501 ttcctgaact cgctgttcag catgttctct gtagagacgg cacgctcttt cagtttcttc
     7561 tcgaagtctg tccccatttg taaaagattg ctcatacagc gcccctttca gcctgagatt
     7621 acgcccgccc tccgggtcgg cgatactgat actgctcctg gttgtcctca ccacctcaaa
     7681 acctgccgct gtaagcgcct cagtgacatc ctgacgcgtt tttagcgctc cggcatggta
     7741 aagagcctcc agtcccctcg taatcgctt
//
