LOCUS       NZ_CP146296             8362 bp    DNA     circular CON 11-MAY-2024
DEFINITION  Shigella sonnei strain R16.0823 plasmid unnamed2, complete sequence.
ACCESSION   NZ_CP146296
VERSION     NZ_CP146296.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40123227
            Assembly: GCF_037043765.2
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8362)
  AUTHORS   Chiou,C.-S., Hong,Y.-P. and Chen,B.-H.
  TITLE     Shigella sonnei
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8362)
  AUTHORS   Chiou,C.-S., Hong,Y.-P. and Chen,B.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-FEB-2024) Center for Diagnostics and Vaccine
            Development, Centers for Disease Control, Taiwan, No.20, Wenxin S.
            3rd Rd., Nantun Dist., Taichung City 408, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.9.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 204.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037043765.2-RS_2024_05_10
            Annotation Date                   :: 05/10/2024 23:57:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,019
            CDSs (total)                      :: 4,890
            Genes (coding)                    :: 4,251
            CDSs (with protein)               :: 4,251
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 639
            CDSs (without protein)            :: 639
            Pseudo Genes (ambiguous residues) :: 0 of 639
            Pseudo Genes (frameshifted)       :: 177 of 639
            Pseudo Genes (incomplete)         :: 435 of 639
            Pseudo Genes (internal stop)      :: 112 of 639
            Pseudo Genes (multiple problems)  :: 83 of 639
            Pseudo Genes (short protein)      :: 3 of 639
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP146296.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8362
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="R16.0823"
                     /db_xref="taxon:624"
                     /plasmid="unnamed2"
                     /collected_by="Taiwan CDC"
     gene            complement(11..647)
                     /gene="tetR(A)"
                     /locus_tag="V8Z64_RS00640"
                     /old_locus_tag="V8Z64_00640"
                     /pseudo=""
     CDS             complement(11..647)
                     /gene="tetR(A)"
                     /locus_tag="V8Z64_RS00640"
                     /old_locus_tag="V8Z64_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /name="tetR(A)"
     gene            753..1950
                     /gene="tet(A)"
                     /locus_tag="V8Z64_RS00645"
                     /old_locus_tag="V8Z64_00645"
                     /pseudo=""
     ARG             679..2285
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="98.67"
                     /coverage="126.18"
     gene            complement(<1917..2105)
                     /locus_tag="V8Z64_RS00650"
                     /old_locus_tag="V8Z64_00650"
                     /pseudo=""
     CDS             complement(<1917..2105)
                     /locus_tag="V8Z64_RS00650"
                     /old_locus_tag="V8Z64_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(2149..2538)
                     /locus_tag="V8Z64_RS00655"
                     /old_locus_tag="V8Z64_00655"
     CDS             complement(2149..2538)
                     /locus_tag="V8Z64_RS00655"
                     /old_locus_tag="V8Z64_00655"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            2756..3196
                     /locus_tag="V8Z64_RS00660"
                     /old_locus_tag="V8Z64_00660"
     CDS             2756..3196
                     /locus_tag="V8Z64_RS00660"
                     /old_locus_tag="V8Z64_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(3941..4090)
                     /locus_tag="V8Z64_RS00665"
                     /old_locus_tag="V8Z64_00665"
     CDS             complement(3941..4090)
                     /locus_tag="V8Z64_RS00665"
                     /old_locus_tag="V8Z64_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            4661..4819
                     /locus_tag="V8Z64_RS00670"
                     /old_locus_tag="V8Z64_00670"
     CDS             4661..4819
                     /locus_tag="V8Z64_RS00670"
                     /old_locus_tag="V8Z64_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            5279..6094
                     /gene="sul2"
                     /locus_tag="V8Z64_RS00675"
                     /old_locus_tag="V8Z64_00675"
     ARG             5280..6091
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            6155..6958
                     /gene="aph(3'')-Ib"
                     /locus_tag="V8Z64_RS00680"
                     /old_locus_tag="V8Z64_00680"
     ARG             6156..6955
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     gene            6958..7794
                     /locus_tag="V8Z64_RS00685"
                     /old_locus_tag="V8Z64_00685"
     CDS             6958..7794
                     /locus_tag="V8Z64_RS00685"
                     /old_locus_tag="V8Z64_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            8100..8341
                     /locus_tag="V8Z64_RS00690"
                     /old_locus_tag="V8Z64_00690"
                     /pseudo=""
     CDS             8100..8341
                     /locus_tag="V8Z64_RS00690"
                     /old_locus_tag="V8Z64_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /name="relaxase"
     ARG             6959..7791
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(4126..4299)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          4223..4828
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gcggtcctga tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc
       61 ttttcgccaa tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg
      121 cttcgtcgaa ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg
      181 tgccgccgcg ctcgccggcc tgctcctcaa gcacggcccc aacagtgaag tagctgattg
      241 tcatcagcgc attgacggcg tccccggccg aaaaacccgc ctcgcagagg aagcgaagct
      301 gcgcgtcggc cgtttccatc tgcggtgcgc ccggtcgcgt cccgcgcatg gatgcgcgcg
      361 ccatcgcggt aggcgagcag cgcctgcctg aagctgcggg cattcccgat cagaaatgag
      421 cgccagtcgt cgtcggctct cggcaccgaa tgcgtatgat tctccgccag catggcttcg
      481 gccagtgcgt cgagcagcgc ccgcttgttc ctgaagtgcc agtaaagcgc cggctgctga
      541 acccccaacc gttccgccag tttgcgtgtc gtcagaccgt ctacgccgac ctcgttcaac
      601 aggtccaggg cggcacggat cactgtattc ggctgcaact ttgtcatgct tgacacttta
      661 tcactgataa acataatatg tccaccaact tatcagtgat aaagaatccg cgcgttcaat
      721 cggaccagcg gaggctggtc cggaggccag acgtgaaacc caacagaccc ctgatcgtaa
      781 ttctgagcac tgtcgcgctc gacgctgtcg gcatcggcct gattatgccg gtgctgccgg
      841 gcctcctgcg cgatctggtt cactcgaacg acgtcaccgc ccactatggc attctgctgg
      901 cgctgtatgc gttgatgcaa tttgcctgcg cacctgtgct gggcgcgctg tcggatcgtt
      961 tcgggcggcg gccggtcttg ctcgtctcgc tggccggcgc tgctgtcgac tacgccatca
     1021 tggcgacggc gcctttcctt tgggttctct atatcgggcg gatcgtggcc ggcatcaccg
     1081 gggcgactgg ggcggtagcc ggcgcttata ttgccgatat cactgatggc gatgagcgcg
     1141 cgcggcactt cggcttcatg agcgcctgtt tcgggttcgg gatggtcgcg ggacctgtgc
     1201 tcggtgggct gatgggcgat ttctcccccc acgctccgtt cttcgccgcg gcagccttga
     1261 acggcctcaa tttcctgacg ggctgtttcc ttttgccgga gtcgcacaaa ggcgaacgcc
     1321 ggccgttacg ccgggaggct ctcaacccgc tcgcttcgtt ccggtgggcc cggggcatga
     1381 ccgtcgtcgc cgccctgatg gcggtcttct tcatcatgca acttgtcgga caggtgccgg
     1441 ccgcgctttg ggtcattttc ggcgaggatc gctttcactg ggacgcgacc acgatcggca
     1501 tttcgcttgc cgcatttggc attctgcatt cactcgccca ggcaatgatc accggccctg
     1561 tagccgcccg gctcggcgaa aggcgggcac tcatgctcgg aatgattgcc gacggcacag
     1621 gctacatcct gcttgccttc gcgacacggg gatggatggc gttcccgatc atggtcctgc
     1681 ttgcttcggg tggcatcgga atgccggcgc tgcaagcaat gttgtccagg caggtggata
     1741 gaagtcaggg gcagctgcaa ggctcactgg cggcgctcac cagcctgacc tcgatcatta
     1801 ggacccctcc tcttcacggc gatctatgcg gcttctataa caacgtggaa cgggtgggca
     1861 tggattgcag gcgctgccct ctacttgctc tgcctgccgg cgctgcgtcg cgggctttgg
     1921 agaaattctt caaattcccg ttgcacatag cccggcaatt cctttccctg ctctgccata
     1981 agcgcagcga atgccgggta atactcgtca acgatctgat agagaagggt ttgctcgggt
     2041 cggtggctct ggtaacgacc agtatcccga tcccggctgg ccgtcctggc cgccacatga
     2101 ggcatgttcc gcgtccttgc aatactgtgt ttacatacag tctatcgctt agcggaaagt
     2161 tcttttaccc tcagccgaaa tgcctgccgt tgctagacat tgccagccag tgcccgtcac
     2221 tcccactctt acattgttcg cagtatctag gtacatgtta ttgcgtgcac cttcatgatg
     2281 tataatgtta ttaatgacag cagaaagatc gacagaatta atattcttcg tacctgggac
     2341 agttatcggg tgtcggccag tgatgagacc aaaggatgca gtataagatg aaccactact
     2401 attatcatgg ccaccattac caccaccgtt aaaactacct cccgaacttc cagagccgct
     2461 atgaccatca ttgtaggggt catttggtcc catgtgacca aaaccaccga cagtaccgct
     2521 atttaactct gtactcataa aattatcctc tttgactttt aaaaacagca aaaactgtat
     2581 ttataacaat gtgtttatat acagtattcg aaatactacg cccgtcaaaa ccacaccgtc
     2641 aacaggtttt ttgtgtattt ttcacgcctc acgggcagcc tcagaccttt tgtgttaaaa
     2701 cagtaatatg ttctgctgtt acaatacggt acacacagga gacactgtca cttttatggc
     2761 atggcacaca gttcaagaga cactgaaatt aacaggaaag tcgcgctcgc aattatatcg
     2821 tgacatggct aaaggccttg tcagctatcg cacaggaaaa aatgatcgcc gggagttcga
     2881 gaccagtgaa ctgattcggg cctatggcga gctgcgcact tctgagacac caaaaaggca
     2941 caaggaggga cactccaaat ctgtcgttag ccaggcggaa aatgctcagc tagacgtcat
     3001 tcagcagaaa ctggacgctc tgcagcagac tgttacccta atgcttgaag acaagatggc
     3061 gcgggaagca gaaaaccgcc tgcgaaatga ggagagggaa caactacagg ccgaaattag
     3121 ccgtctggct gacgcgctgg cacaggaaaa aaagcgggga ttctggtcgc ggctgtttcg
     3181 ctccggttca gagtaacagt gcaggtgact ctgatttacc ataacatctg ttatcagcac
     3241 cgcctgagag gccctcaggg cgtttctgac gggaaaccgg taaaacaggc aacaaaccac
     3301 ccgaaaaccc gccagaaacg cgcaggctct gtttttacgg catgcatgag tatgcgcaca
     3361 agtgcatagc gcggaaattc acaccattca gccggggtaa gtcctgtttc cggcgttctt
     3421 cccatccgaa aacggtgaac ggggacgcaa tttattgcgt ggccgtgaag cgttgccggc
     3481 gcagccggaa aacccgctgt cagcacgttc aggaaggggg tgatgtacaa atggcacaga
     3541 ggatgaaagt gacgtatgag ctgaaggaat gatgtaaaag tgatgtataa gtgatgtaaa
     3601 agtgatgtat gaactgaagg agcgatgtaa ttctgtagtc aggaagaata acccgttcat
     3661 gcaggcatga acgatacagt gaggaacgct ttttttgtca cgatctcccc ttaacctgcg
     3721 taaatcaccc gccatggttg attatttttc aacctcaaaa tggtcataca cctaacgcag
     3781 ccgcgatttt catcgcaact gcatgaatta aaagaaaaaa aaggaatatt tcgttttcac
     3841 gagtaatacc cgggtattaa aaatccactg taattacatg aaaataaaga aataaaaggt
     3901 aataccggag tattaccacg tacgcgggca agcgaaaaag ctataacgct tcccggccga
     3961 caggcgaccc aaaatccaca ggggcatgca ggttctcttc agtaacctgc gccattaaat
     4021 caaaaaggtc atattcgccg ggaagctgcg tatcagtcga atccagttgt tcatcatatt
     4081 cagatggcat ggggtgatat ccagtagctg agaggttgtt agcgggtttc ggggcgcagc
     4141 cctgaaccag tcatgtagcg ctagcggagt gtatactggc ttactatgtt gccacgattg
     4201 cgaaaatcag tgaagtgctt catgtggcag gagaaaaaag gcagcaccgg cgcgtcagca
     4261 gaatatgcga tacaggagat attccgcttc ctcgctcact gactcgctac gctcggtcgt
     4321 tcggctgcgg cgagcggtac cggcttacgg acggggacgg aaatttcctg gaagatacca
     4381 ggaagacact tagcaaagaa gagataaggc cacggcgaag ccgtttttcc ataggctccg
     4441 cccccctgac aagcatcgca aaatctgacg ctcaaatcag tggtggcgaa acccgacagg
     4501 actataaaga tcccaggcgt ttccccctgg tagctccctc gtgcgctctc ctgttcctgc
     4561 ctttcggttt accggtgtca ttccgctgtt atggccgcgg tttctcattc cacgcctgac
     4621 actcagttcc gtgaaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca
     4681 gtccgaccgc tgcgccttgt ccggtaacta tcaacttgag tccaacccgg aaagacacga
     4741 caaaacgcca ctggcagcag ccattagtaa caggagtgca cgagaagata gattaagagg
     4801 tcttgaagta gaggcctgac tgcggctaca ctgaaagaac agttttggta atggcggtct
     4861 cgtgaaccct gttaccgggt taagcagttc cccaactgac ttaaccttcg aacaaaccgc
     4921 ctccccaggc ggttttttcg tttacagagc aagagattac gattcattga aaaggatctc
     4981 aagaagatcc tttgtagatt actatcaatt aatcttccga acatgacacc agtcactgtt
     5041 gattacggtt aaaccgctcc gtaacaggtt tccctcgtat tggtgacttg cagcaccaaa
     5101 gaagcaaaaa agataaatat atctacttat tgagagatga tatatttatt tccaaaatgc
     5161 tacagataca tcatcatgaa acggatcacc gcaaacaggt tactcgcctg gggattccct
     5221 ttcgacccga gcatccgtat gagactcatg ctcgattatt attattatag aagcccccat
     5281 gaataaatcg ctcatcattt tcggcatcgt caacataacc tcggacagtt tctccgatgg
     5341 aggccggtat ctggcgccag acgcagccat tgcgcaggcg cgtaagctga tggccgaggg
     5401 ggcagatgtg atcgacctcg gtccggcatc cagcaatccc gacgccgcgc ctgtttcgtc
     5461 cgacacagaa atcgcgcgta tcgcgccggt gctggacgcg ctcaaggcag atggcattcc
     5521 cgtctcgctc gacagttatc aacccgcgac gcaagcctat gccttgtcgc gtggtgtggc
     5581 ctatctcaat gatattcgcg gttttccaga cgctgcgttc tatccgcaat tggcgaaatc
     5641 atctgccaaa ctcgtcgtta tgcattcggt gcaagacggg caggcagatc ggcgcgaggc
     5701 acccgctggc gacatcatgg atcacattgc ggcgttcttt gacgcgcgca tcgcggcgct
     5761 gacgggtgcc ggtatcaaac gcaaccgcct tgtccttgat cccggcatgg ggttttttct
     5821 gggggctgct cccgaaacct cgctctcggt gctggcgcgg ttcgatgaat tgcggctgcg
     5881 cttcgatttg ccggtgcttc tgtctgtttc gcgcaaatcc tttctgcgcg cgctcacagg
     5941 ccgtggtccg ggggatgtcg gggccgcgac actcgctgca gagcttgccg ccgccgcagg
     6001 tggagctgac ttcatccgca cacacgagcc gcgccccttg cgcgacgggc tggcggtatt
     6061 ggcggcgctg aaagaaaccg caagaattcg ttaactgcac attcgggata tttctctata
     6121 ttcgcgcttc atcagaaaac tgaaggaacc tccattgaat cgaactaata ttttttttgg
     6181 tgaatcgcat tctgactggt tgcctgtcag aggcggagaa tctggtgatt ttgtttttcg
     6241 acgtggtgac gggcatgcct tcgcgaaaat cgcacctgct tcccgccgcg gtgagctcgc
     6301 tggagagcgt gaccgcctca tttggctcaa aggtcgaggt gtggcttgcc ccgaggtcat
     6361 caactggcag gaggaacagg agggtgcatg cttggtgata acggcaattc cgggagtacc
     6421 ggcggctgat ctgtctggag cggatttgct caaagcgtgg ccgtcaatgg ggcagcaact
     6481 tggcgctgtt cacagcctat cggttgatca atgtcagttt gagcgcaggc tgtcgcgaat
     6541 gttcggacgc gccgttgatg tggtgtcccg caatgccgtc aatcccgact tcttaccgga
     6601 cgaggacaag agtacgccgc agctcgatct tttggctcgt gtcgaacgag agctaccggt
     6661 gcggctcgac caagagcgca ccgatatggt tgtttgccat ggtgatccct gcatgccgaa
     6721 cttcatggtg gaccctaaaa ctcttcaatg cacgggtctg atcgaccttg ggcggctcgg
     6781 aacagcagat cgctatgccg atttggcact catgattgct aacgccgaag agaactgggc
     6841 agcgccagat gaagcagagc gcgccttcgc tgtcctattc aatgtattgg ggatcgaagc
     6901 ccccgaccgc gaacgccttg ccttctatct gcgattggac cctctgactt ggggttgatg
     6961 ttcatgccgc ctgtttttcc tgctcattgg cacgtttcgc aacctgttct cattgcggac
     7021 accttttcca gcctcgtttg gaaagtttca ttgccagacg ggactcctgc aatcgtcaag
     7081 ggattgaaac ctatagaaga cattgctgat gaactgcgcg gggccgacta tctggtatgg
     7141 cgcaatggga ggggagcagt ccggttgctc ggtcgtgaga acaatctgat gttgctcgaa
     7201 tatgccgggg agcgaatgct ctctcacatc gttgccgagc acggcgacta ccaggcgacc
     7261 gaaattgcag cggaactaat ggcgaagctg tatgccgcat ctgaggaacc cctgccttct
     7321 gcccttctcc cgatccggga tcgctttgca gctttgtttc agcgggcgcg cgatgatcaa
     7381 aacgcaggtt gtcaaactga ctacgtccac gcggcgatta tagccgatca aatgatgagc
     7441 aatgcctcgg aactgcgtgg gctacatggc gatctgcatc atgaaaacat catgttctcc
     7501 agtcgcggct ggctggtgat agatcccgtc ggtctggtcg gtgaagtggg ctttggcgcc
     7561 gccaatatgt tctacgatcc ggctgacaga gacgaccttt gtctcgatcc tagacgcatt
     7621 gcacagatgg cggacgcatt ctctcgtgcg ctggacgtcg atccgcgtcg cctgctcgac
     7681 caggcgtacg cttatgggtg cctttccgca gcttggaacg cggatggaga agaggagcaa
     7741 cgcgatctag ctatcgcggc cgcgatcaag caggtgcgac agacgtcata ctagatatca
     7801 agcgacttct cctatcccct gggaacacat caatctcacc ggagaatatc gctggccaaa
     7861 gccttagcgt aggattccgc cccttcccgc aaacgacccc tggaagcccc gtagcgacgc
     7921 ggagaggggc gagacaagcc aagggcgcag gctcgatgcg cagcacgaca tagccggttc
     7981 tcgcaaggac gagaatttcc ctgcggtgcc cctcaagtgt caatgaaagt ttccaacgcg
     8041 agccattcgc gagagccttg agtccacgct agatctatct catctgcgca aggcagaacg
     8101 tgaagacggc cgccctggac ctcgcccgcg agcgccaggc gcacgaggcc ggcgcgcgga
     8161 cccgcgccac ggcccacgag cggacgccgc agcaggagcg ccagaaggcc gccagagagg
     8221 ccgagcgcgg ccgtgaggct tggacgctag ggcagggcat gaaaaagccc gtagcgggct
     8281 gctacgggcg tctgacgcgg tggaaagggg aggggatgtt gtctacatgg ctctgctgta
     8341 gtgagtgggt tgcgctccgg ca
//
