LOCUS       NZ_CP146289            10216 bp    DNA     circular CON 19-MAY-2024
DEFINITION  Shigella sonnei strain R15.3427 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP146289
VERSION     NZ_CP146289.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN40123130
            Assembly: GCF_037042485.2
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10216)
  AUTHORS   Chiou,C.-S., Hong,Y.-P. and Chen,B.-H.
  TITLE     Shigella sonnei
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10216)
  AUTHORS   Chiou,C.-S., Hong,Y.-P. and Chen,B.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-FEB-2024) Center for Diagnostics and Vaccine
            Development, Centers for Disease Control, Taiwan, No.20, Wenxin S.
            3rd Rd., Nantun Dist., Taichung City 408, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.9.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 194.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037042485.2-RS_2024_05_19
            Annotation Date                   :: 05/19/2024 13:20:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,192
            Genes (coding)                    :: 4,465
            CDSs (with protein)               :: 4,465
            Genes (RNA)                       :: 132
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 98
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 727
            CDSs (without protein)            :: 727
            Pseudo Genes (ambiguous residues) :: 0 of 727
            Pseudo Genes (frameshifted)       :: 209 of 727
            Pseudo Genes (incomplete)         :: 519 of 727
            Pseudo Genes (internal stop)      :: 128 of 727
            Pseudo Genes (multiple problems)  :: 119 of 727
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP146289.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="R15.3427"
                     /db_xref="taxon:624"
                     /plasmid="unnamed3"
                     /collected_by="Taiwan CDC"
     gene            72..167
                     /locus_tag="V8Z63_RS00005"
                     /old_locus_tag="V8Z63_00005"
     CDS             72..167
                     /locus_tag="V8Z63_RS00005"
                     /old_locus_tag="V8Z63_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /GO_process="GO:0046677 - response to antibiotic [Evidence
                     IEA]; GO:0031556 - transcriptional attenuation by ribosome
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="WP_013362819.1"
                     /translation="MLVFQMRYQMRYVDKTSTVLKQTKKSDYADK"
                     /name="23S rRNA methyltransferase attenuation leader
                     peptide"
     gene            293..1030
                     /gene="erm(B)"
                     /locus_tag="V8Z63_RS00010"
                     /old_locus_tag="V8Z63_00010"
     ARG             294..1027
                     /name="erm(B)"
                     /gene="erm(B)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="99.59"
                     /coverage="100.0"
     gene            1660..2109
                     /locus_tag="V8Z63_RS00015"
                     /old_locus_tag="V8Z63_00015"
     CDS             1660..2109
                     /locus_tag="V8Z63_RS00015"
                     /old_locus_tag="V8Z63_00015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_013362817.1"
                     /translation="MTTILSDYNKSILIELLNTNRQIIVVHGDDIDDYYTDSCYDIIFI
                     DYYDDDDFTYNGQEIWRGDSIYLVKSYQDEIQNRIEKGQELIFVNKENDISFSESILTY
                     YHLFDYAGKTFEIDGQIIDEQTKVFAAPERRCACRTCWKVPARKT"
                     /name="hypothetical protein"
     gene            2070..2411
                     /locus_tag="V8Z63_RS00020"
                     /old_locus_tag="V8Z63_00020"
     CDS             2070..2411
                     /locus_tag="V8Z63_RS00020"
                     /old_locus_tag="V8Z63_00020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin"
                     /protein_id="WP_023909256.1"
                     /translation="MSHVLEGASQEDLNLYRAEVERDQAYGNWRDFFLNKKGSVTSVSG
                     DANLDQIADVSYLLDTFFAKVTRTALQNAASIAGLMITTEAMVAEAPKKDEPAMPPGGG
                     MGGMGGMDF"
                     /name="chaperonin"
     gene            2686..4170
                     /locus_tag="V8Z63_RS00025"
                     /old_locus_tag="V8Z63_00025"
     CDS             2686..4170
                     /locus_tag="V8Z63_RS00025"
                     /old_locus_tag="V8Z63_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="WP_032152108.1"
                     /translation="MPSAHTGSRYARHAPERTLLYALVEAHYPDFIARIEAEGRSLPGY
                     VREAFDAYLRCGVLEHGFLRVVCEHCRAERLVAFSCKKRGFCPSCGARRMAESARHLVE
                     EVFGPRPVRQWVLSFPYPLRFLFASKPEAIGPVLGIVQRVIAGWLADQAGIDRASAQCG
                     AVTLIQRFGSALNLNIHFHMLWLDGVYVEATELPRRELRLHRARAPTTAQLTQLAAAIA
                     HRVCRHLTRKGWLEGEGESAFLADSAAGDDSMDGLRMSSITYRIATGRDAGCKVVTLQT
                     LPGDAGSLEGEAGKVGGFSLHAGVAAEAHESHKLEKLCRYITRPAISEKRLSIALQGRV
                     RYQLKTPWRNGTTHVEWDPVDFIAKLAALVPPPRAHLTRFHGVFAPNAALRAQLTPSGR
                     GRRHDAAVEPADASANDAPRSPEEKRRSMSWAQRLKRVFSIDVTACVHCGGTVRIVASI
                     EEPAAIRAILGHFVKQGAREEAHYRPAARAPPVQAA"
                     /name="IS91 family transposase"
     gene            complement(4493..5197)
                     /locus_tag="V8Z63_RS00030"
                     /old_locus_tag="V8Z63_00030"
     CDS             complement(4493..5197)
                     /locus_tag="V8Z63_RS00030"
                     /old_locus_tag="V8Z63_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            5337..6101
                     /locus_tag="V8Z63_RS00035"
                     /old_locus_tag="V8Z63_00035"
     CDS             5337..6101
                     /locus_tag="V8Z63_RS00035"
                     /old_locus_tag="V8Z63_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="WP_001389365.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDHT
                     TIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTIDFY
                     LSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETAHRQ
                     VKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQPGIR
                     GEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
                     /name="IS6-like element IS6100 family transposase"
     gene            complement(6594..7178)
                     /gene="mphR(A)"
                     /locus_tag="V8Z63_RS00040"
                     /old_locus_tag="V8Z63_00040"
     CDS             complement(6594..7178)
                     /gene="mphR(A)"
                     /locus_tag="V8Z63_RS00040"
                     /old_locus_tag="V8Z63_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="WP_001137892.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAALI
                     QRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVNYL
                     ISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVDPDG
                     ELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
                     /name="mphR(A)"
     gene            complement(7178..8416)
                     /gene="mrx(A)"
                     /locus_tag="V8Z63_RS00045"
                     /old_locus_tag="V8Z63_00045"
                     /gene_synonym="mrx"
     CDS             complement(7178..8416)
                     /gene="mrx(A)"
                     /locus_tag="V8Z63_RS00045"
                     /old_locus_tag="V8Z63_00045"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="WP_000004159.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWAG
                     ATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTGLI
                     ACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGAAIG
                     LLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLLRPLF
                     GIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVGARISA
                     QNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRTPPELLG
                     RVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQFATMAAA
                     ATASAPTHTEGEH"
                     /name="mrx(A)"
     gene            complement(8413..9327)
                     /locus_tag="V8Z63_RS00050"
                     /old_locus_tag="V8Z63_00050"
     CDS             complement(8413..9327)
                     /locus_tag="V8Z63_RS00050"
                     /old_locus_tag="V8Z63_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="WP_023063803.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVDD
                     GRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMV
                     IQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDV
                     DRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSE
                     ARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFA
                     LDSGNEEYLAAAKAQLAAAEAAE"
                     /name="Mph(A) family macrolide 2'-phosphotransferase"
     gene            complement(9449..10153)
                     /locus_tag="V8Z63_RS00055"
                     /old_locus_tag="V8Z63_00055"
     CDS             complement(9449..10153)
                     /locus_tag="V8Z63_RS00055"
                     /old_locus_tag="V8Z63_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_142975730.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKDIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     ARG             complement(8426..9327)
                     /name="mph(A)"
                     /gene="mph(A)"
                     /gene_name="Mph(A) family macrolide 2'-phosphotransferase"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(8414..9327)
                     /name="mphA"
                     /gene="mphA"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="100.0"
                     /coverage="101.0"
     ARG             294..1030
                     /name="ErmB"
                     /gene="ErmB"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="97.96"
                     /coverage="98.79"
     ARG             complement(7179..8416)
                     /name="Mrx"
                     /gene="Mrx"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 aaattttgta taataggaat tgaagttaaa ttagatgcta aaaatttgta attaagaagg
       61 agggattcgt catgttggta ttccaaatgc gttatcaaat gcgttatgta gataaaacat
      121 ctactgtttt gaaacagact aaaaaaagtg attacgcaga taaataaata cgttagatta
      181 attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt acaaacaaat
      241 cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtaaatt acatgaacaa
      301 aaatataaaa tattctcaaa actttttaac gagtgaaaaa gtactcaacc aaataataaa
      361 acaattgaat ttaaaagaaa ccgataccgt ttacgaaatt ggaacaggta aagggcattt
      421 aacgacgaaa ctggctaaaa taagtaaaca ggtaacgtct attgaattag acagtcatct
      481 attcaactta tcgtcagaaa aattaaaact gaatactcgt gtcactttaa ttcaccaaga
      541 tattctacag tttcaattcc ctaacaaaca gaggtataaa attgttggga atattcctta
      601 ccatttaagc acacaaatta ttaaaaaagt ggtttttgaa agccatgcgt ctgacatcta
      661 tctgattgtt gaagaaggat tctacaagcg taccttggat attcaccgaa cactagggtt
      721 gctcttgcac actcaagtct cgattcagca attgcttaag ctgccagcgg aatgctttca
      781 tcctaaacca agagtaaaca gtgtcttaat aaaacttacc cgccatacca cagatgttcc
      841 agataaatat tggaagctat atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg
      901 tcaactgttt actaaaaatc agtttcatca agcaatgaaa cacgccaaag taaacaattt
      961 aagtaccgtt acttatgagc aagtattgtc tatttttaat agttatctat tatttaacgg
     1021 gaggaaataa ttctatgagt cgcttttgta aatttggaaa gttacacgtt actaaaggga
     1081 atgtagataa attattaggt atactactga cagcttccaa ggagctaaag aggtccctag
     1141 actagcaaga agtacacaag aagccttaaa gattatagaa aagcttttcc atgaaatgct
     1201 taacattatt taatgttaac atgtggcttt gtggtaacta ccacgagtgg taacttgtaa
     1261 catgaaaaaa gttacaagtt acaagtggtt tatttacgaa cataaaggct aggaaagcac
     1321 ttgataagta tatcaagtgc tttttttccg ttcgatttta ctatccaaga taccgcttga
     1381 cgtacctaat attttagtgt gctaagggct ttgctttttc tggttatagt tcgttcaaaa
     1441 aagaccgcaa gggtaaatgc acgatcatag atcgcccttg cagcttttta atctctccac
     1501 ttttttataa ccaagcaaac caccaagcac actgaaaaaa taattaggaa ctaaagcgaa
     1561 gtgcgtatct tggataaatc ttcactcgat tttaaaagat taagcaccca gccaaagcgc
     1621 ctaaaaaaat aattttttta aagaaaggaa gtaatttaaa tgactacaat tttaagcgac
     1681 tacaataaat ctatcctaat tgaattacta aacaccaatc gccaaattat cgtcgttcat
     1741 ggcgacgata ttgatgatta ttacacagat tcttgctatg acattatctt tattgattac
     1801 tatgatgatg atgatttcac ttataacgga caggaaatat ggcgtggcga tagcatttat
     1861 cttgtaaaaa gttatcaaga tgaaattcaa aaccgtatag aaaaaggaca agagctgata
     1921 tttgttaata aagaaaatga tatatcattt tcagaatcta ttttaactta ttaccatcta
     1981 tttgattatg ctggtaaaac gtttgaaata gacggtcaaa tcattgatga acaaacaaaa
     2041 gtttttgccg cacccgagcg ccgctgcgca tgtcgcacgt gctggaaggt gccagccagg
     2101 aagacctgaa cctttaccgc gcggaagtgg agcgcgacca ggcctatggc aactggcgcg
     2161 atttcttcct gaacaagaag ggcagcgtca catccgtgag cggtgatgcc aacctcgacc
     2221 agatcgcgga cgtgtcgtac ctgctggata ccttctttgc caaggtcacc cgcaccgcgt
     2281 tgcagaacgc cgcctccatc gcgggcctga tgatcaccac cgaagccatg gtggccgagg
     2341 ccccgaagaa ggacgagccg gcgatgccgc cgggcggcgg catgggtggc atgggcggca
     2401 tggatttctg atgcggttgg cccggtcgtc agggaaccgg accgcgccag cgcggtccga
     2461 tcccggcaac gacccgacat caaggcccca aggacggggc cggagcccgg cagcgatgcc
     2521 gggctttttg ttgtgcccgc gccgcggcaa tgtctgacgc gaagatcaga acgcaccgat
     2581 acgaacgtgc gaacacaggt gcaaccctga gcagccgtcc ccgcaccgga gcgctgcgtg
     2641 ccgcgcctcg ccacatcccg gcggcaagcc gcgggatgcg cgccactgcc gtccgcccac
     2701 accggttcgc ggtacgcgcg ccacgcgccc gagcgcacgc tgctgtacgc gttggtagag
     2761 gcgcactacc cggacttcat tgcacggatc gaagcggagg gccgctcgct gcccgggtat
     2821 gtccgcgagg cgttcgatgc ctacctgcgt tgcggcgtgc tcgagcacgg cttcctgcgg
     2881 gtggtgtgcg agcactgccg tgcagagagg ctggtggcct tctcctgcaa gaagcgcggg
     2941 ttctgcccga gttgcggcgc gcgacgcatg gccgagagtg cgcggcacct ggtcgaggag
     3001 gtgttcggcc cgcggcctgt gcggcaatgg gtgctgagct ttccgtaccc cttgcgtttc
     3061 ctgttcgcca gcaagccaga agccattggc ccggtgctgg gcatcgtgca gcgcgtgatc
     3121 gccggctggt tggccgatca agccggcatc gaccgcgcca gcgcccagtg cggcgcggtg
     3181 acgctgatcc agcgtttcgg cagcgcgctg aacctgaaca tccacttcca catgctgtgg
     3241 ctcgacggcg tgtacgtgga agccaccgag ctgccgcggc gcgaactgcg cctgcaccgc
     3301 gcccgtgcgc ccaccaccgc gcagttgacc cagctggcag ctgccatcgc gcaccgggtg
     3361 tgtcggcacc tgacgcgcaa aggctggctc gaaggggagg gcgaatcggc cttcctggca
     3421 gacagcgctg caggcgacga cagcatggat gggctgcgga tgagttcgat cacctaccgc
     3481 atcgccaccg gccgcgacgc tggctgcaag gtcgtcacgc tgcaaacgct gcccggtgac
     3541 gccggttcgc tggagggcga agccggcaag gtcggcggct tctcactgca tgccggcgtg
     3601 gcggccgaag cacacgaaag ccacaagctg gaaaagctgt gccgctacat cacgcgcccg
     3661 gcgatcagcg agaagcggct gtcgatagcg ctccagggca gggtgcgtta ccagctcaag
     3721 accccgtggc gcaatggcac cacgcatgtg gaatgggatc cggtggattt catcgccaag
     3781 ctggcggcgc tggtcccgcc accgcgcgcc cacctgaccc gcttccacgg ggtgtttgcc
     3841 ccgaacgccg ccctgcgcgc acagctgacg ccatcggggc gcggcaggcg gcatgacgcc
     3901 gctgtggagc cggcggacgc aagcgcgaac gacgcgccgc gcagccccga ggagaagcgc
     3961 cgttcgatga gctgggcgca acgcctcaag cgggtctttt ccatcgacgt caccgcctgc
     4021 gtccactgcg gtggcaccgt gcggatcgtc gccagcatcg aggaacctgc cgccatccgc
     4081 gccatccttg gccacttcgt gaagcagggc gcgcgggaag aagcgcacta caggcccgca
     4141 gcgcgcgcac cgccagtgca agccgcgtga cgatctgccg gctgcacagc cgacggcgaa
     4201 accggaatcc gagccgatgc ggccacgatc cgcagggcgg cgctcggccc gctgtcggga
     4261 atcagcgaag catggctgct gacaacgccg ctgcgtggcc ccgcgatgcc gaaatcccac
     4321 tcacagacgt ccgatccgtg cccaaaacgg ggcttgcgcg accgccgcct acccagcaga
     4381 ctgcccgaaa agggcgtttg aacttcctat acgctgatag tgcattatct taaaattttt
     4441 ggcactgttg caaagttagc gatgaggcag ccttttgtct tattcaaagg ccttacattt
     4501 caaaaactct gcttaccagg cgcatttcgc ccaggggatc accataataa aatgctgagg
     4561 cctggccttt gcgtagtgca cgcatcacct caataccttt gatggtggcg taagccgtct
     4621 tcatggattt aaatcccagc gtggcgccga ttatccgttt cagtttgcca tgatcgcatt
     4681 caatcacgtt gttccggtac ttaatctgtc ggtgttcaac gtcagacggg caccggcctt
     4741 cgcgtttgag cagagcaagc gcgcgaccat aggcgggcgc tttatccgtg ttgatgaatc
     4801 gcgggatctg ccacttcttc acgttgttga ggattttacc cagaaaccgg tatgcagctt
     4861 tgctgttacg acgggaggag agataaaaat cgacagtgcg gccccggctg tcgacggccc
     4921 ggtacagata cgcccagcgg ccattgacct tcacgtaggt ttcatccatg tgccacgggc
     4981 aaagatcgga agggttacgc cagtaccagc gcagccgttt ttccatttca ggcgcataac
     5041 gctgaaccca gcggtaaatc gtggagtgat cgacattcac tccgcgttca gccagcatct
     5101 cctgcagctc acggtaactg atgccgtatt tgcagtacca gcgtacggcc cacagaatga
     5161 tgtcacgctg aaaatgccgg cctttgaatg ggttcatgtg cagctccatc agcaaaaggg
     5221 gatgataagt ttatcaccac cgactatttg caacagtgcc ctcaatactc gtgtgggctc
     5281 tgttgcaaaa atcgtgaagc ttgagcatgc ttggcggaga ttggacggac ggaacgatga
     5341 cggatttcaa gtggcgccat ttccagggtg atgtgatcct gtgggcggtg cgctggtatt
     5401 gtcgctatcc gatcagctat cgcgaccttg aggaaatgct ggcggaacgc ggcatttcgg
     5461 tcgaccatac gacgatctat cgctgggtcc agtgctacgc cccggagatg gagaagcggc
     5521 tgcgctggtt ctggcggcgt ggctttgatc cgagctggcg cctggatgaa acctacgtca
     5581 aggtgcgggg caagtggacc tacctgtacc gggcagtcga caagcggggc gacacgatcg
     5641 atttctacct gtcgccgacc cgcagcgcca aggcagcgaa gcggttcctg ggcaaggccc
     5701 tgcgaggcct gaagcactgg gaaaagcctg ccacgctcaa taccgacaaa gcgccgagct
     5761 atggtgcagc gatcaccgaa ttgaagcgcg aaggaaagct ggaccgggag acggcccacc
     5821 ggcaggtgaa gtatctcaat aacgtgatcg aggccgatca cggaaagctc aagatactga
     5881 tcaagccggt gcgcggtttc aaatcgatcc ccacggccta tgccacgatc aagggattcg
     5941 aagtcatgcg agccctgcgc aaaggacagg ctcgcccctg gtgcctgcag cccggcatca
     6001 ggggcgaggt gcgccttgtg gagagagctt ttggcattgg gccctcggcg ctgacggagg
     6061 ccatgggcat gctcaaccac catttcgcag cagccgcctg atcggcgcag agcgacagcc
     6121 tacctctgac tgccgccaat ctttgcaaca gagcccgccg tgctagtctg ctcggtgatg
     6181 gtggagtgaa gccaacccgc aatcgggtta tgaatctgca tcgcgattcg caatcagctg
     6241 tctcttgagc atgtcgaact cctgcgatgt tatctcgccg tgctcgtgct gctacaccaa
     6301 cttgtccaac tcatccgcca caccgctccc caccacctgc gatggccagg ttgtttgctg
     6361 ggcgcattgg cggagtatcg tctcgacccg ggacacccac accggcacaa ccttacggga
     6421 gtgattcact gtcaaagaat cggcccggtg ctctgacgca agtatcggga tggtcaccat
     6481 ttgtaagccg tagacctgag tggtgatcaa gacttcgata ccaccgaccg taccggtact
     6541 aatcgacgac ggtcgtgttc gtcgcctgcc gcagggactc tgcacacctc cgtttacgca
     6601 tgtgcctgga ggagttggaa atcgtcgtgt tcgggaaaca ttaaacacag gatggcagcg
     6661 atctgagcca gcacatgatc agctagctca ccatccggat cgacggccca ctgcatcgtc
     6721 gcgccagcga tgaccgagtg caggagcaac tcagctgccg caggagcacc tgggggcagt
     6781 cgcttgcgga tcccctccac caccgcgcgg ttccgctgga tcgcaagcgt gcgtagctcc
     6841 ggcacctgga gctcgtacca ggagatgaga tagttcaccg agaagtcgtt gcgagtgttc
     6901 atgctccgaa cgagcacctg caaaaattcc cagagccctt gcggccctgc gcctatcggt
     6961 atcgcattca ggtaatgccg cacctgctcg acgccgcgct ccatcatcct caccagcagc
     7021 gtatcgcggt tggtgaagcg ctggattaac gctgcgcggg agagccccac ctcctttgct
     7081 actccgctga gcgtgaactc tatgggaccg caacgcttca gcactacggt ggcggcctcg
     7141 agtacctcgt catcggactt gagcttgggg cggggcatca gtgttcacct tctgtatggg
     7201 ttggggcgga ggctgtggct gccgccgcca ttgtagcaaa ttgaagacgg agcgagagta
     7261 gagccacgag ccccgcaaac acggccgata caacgaggcc agggagcgga ccagcaaggt
     7321 cgacaaacgc gccggccgca agcataacca tgggcgaggc tgacagcatc accgccgaga
     7381 ccgtgccgag tacccggccg agaagttctg gcggcgtgcg gttgtagatg gcagcgttga
     7441 gaatgggaga gactgagccg gtcagcagtc ccacgagcgc gcccaacaac atcagcaccg
     7501 gcacgcctgg caactgtgaa agcagaagcg agcccaccgc agagccacaa aatgccaccg
     7561 ccagccagtt ctgcgctgat atccgggcgc cgaccgacgc atgaatggca atgccaagga
     7621 gaccaccagc ccccatcatt gaggagaaca gcccgagctc tgctacttgg cgtcctgcat
     7681 ctacaaacag cgcaggcatg atgacgctgc cgttggcgcc aacgatgccc acgaagatca
     7741 tcactatacc aaagagaggg cgcagcaggg gttcgctcca gagaaaagcg acgccggcgc
     7801 gcatggagag agtcgccgtc gtggtcatcg tccgagcggc acgcgcggga agcacccacg
     7861 cgccgagcag acctgcaagg acggagcaga acgccgtcag cccgagcgtt ggcgcagcgc
     7921 caagcaggcc gattgcggcc cccccaaggg ccgggccacc tagaatcgcg acgttcccga
     7981 tcaccgcttt cagtgacgag acgcgctcaa cggagagccc ggcgacgtgg ccgagtttgg
     8041 gcagctcact gtcctgcgcg gccataccgg gtgcgtcgaa cgcggcaccg agcaccacgc
     8101 aagcgatcag cccagtgttc gagagggcgc caacggcatc gagcagtggg atgctcgcca
     8161 tggccacgcc gcccaccaca cccgagatca atgcgacggg cgcgcgcccg aaccgatcga
     8221 cgaggccacc accaacccac gcgccgatga tggtcgcgat gacgctgcta gcggccgtgg
     8281 cgcccgccca ggccgcgctc tttgtatgag acaggacgaa ccatggaagc gcgagggccg
     8341 ccaccgcgtt gccgatccgg aagagaaagg tcgccgcgaa cagcgtcgcg agcgggctat
     8401 atcgacgttc gctcattccg ctgcttccgc tgcggcgagc tgcgccttcg ccgcagcgag
     8461 gtactcttcg ttacccgagt cgagggcgaa gagtgcgtag gtgaccgccc cgaacgcaag
     8521 gcgctccgcg atgtggtggg cgagccgcgg ccacacccgg ccaccggccg cttcatacgt
     8581 gaggaggagc ttcgcgagcc cctcttcacc aaagaccata aggtgcgcgg ccatgtcgat
     8641 ggcagggtca tcaacgcggg cctcgctcca gtcgatcatc ccgctgacgc gctccgtgtt
     8701 gtcgatgagc acatggccca cgtagagatc gccatgcacc accacggaga aatctggcca
     8761 cgacgaatcg tcgtcgagcc agcgctgcca ccggtggagg cgcttgtcgt tcaccacgaa
     8821 ctcgcgtcgg acgcggtcaa cgtcgtcggc caccttctga cgggcctgcg tcggtgtacg
     8881 gatgagcatc cccgcatcca cggcggcgga aatggggacg gcatgcaggg cggcgagcgc
     8941 ggtcgcgaag ctctccgcga agacctccga gtcctgcggc acgacccagt cgggcgtgga
     9001 cgaaccaggc tggatgacca tcgcagtcga gtcttcgagc atgggatagg caacgagctc
     9061 ggcgttggcc acgcgccagt ccggcaccgc gaacggcagg cgattcttga gcattgccag
     9121 cacccgcgcc tctggttcga ccttcgcgct tacctcggct cggcgcggga tgcgcagcac
     9181 ccaccgacgt ccatcgtcga cggtggcgat cacgatccta tagtcgagcc caagctcatt
     9241 gacagtcagc gggccatgga gcttgagccc atgtcgggct gcaagtgcgt acagttggga
     9301 ggtatcggcg gtcgtgacta cggtcatgat tcactcctga gggcttgacg ggtttagcca
     9361 cctaaatgta acagtcacgt cggttatatt caatccggca ctgttgcaaa gttagcgatg
     9421 aggcagcctt ttgtcttatt caaaggcctt acatttcaaa aactctgctt accaggcgca
     9481 tttcgcccag gggatcacca taataaaatg ctgaggcctg gcctttgcgt agtgcacgca
     9541 tcacctcaat atctttgatg gtggcgtaag ccgtcttcat ggatttaaat cccagcgtgg
     9601 cgccgattat ccgtttcagt ttgccatgat cgcattcaat cacgttgttc cggtacttaa
     9661 tctgtcggtg ttcaacgtca gacgggcacc ggccttcgcg tttgagcaga gcaagcgcgc
     9721 gaccataggc gggcgcttta tccgtgttga tgaatcgcgg gatctgccac ttcttcacgt
     9781 tgttgaggat tttacccaga aaccggtatg cagctttgct gttacgacgg gaggagagat
     9841 aaaaatcgac agtgcggccc cggctgtcga cggcccggta cagatacgcc cagcggccat
     9901 tgaccttcac gtaggtttca tccatgtgcc acgggcaaag atcggaaggg ttacgccagt
     9961 accagcgcag ccgtttttcc atttcaggcg cataacgctg aacccagcgg taaatcgtgg
    10021 agtgatcgac attcactccg cgttcagcca gcatctcctg cagctcacgg taactgatgc
    10081 cgtatttgca gtaccagcgt acggcccaca gaatgatgtc acgctgaaaa tgccggcctt
    10141 tgaatgggtt catgtgcagc tccatcagca aaaggggatg ataagtttat caccaccgac
    10201 tatttgcaac agtgcc
//
