LOCUS       NZ_CP146197             5596 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain kp1 plasmid unnamedG, complete
            sequence.
ACCESSION   NZ_CP146197
VERSION     NZ_CP146197.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39724753
            Assembly: GCF_037024855.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Li,Z. and Song,G.
  TITLE     A Genomic Study of Clinical Klebsiella pneumoniae Carrying blaOXA-48
            like
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Li,Z. and Song,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2024) clinical laboratory, The First Affiliated
            Hospital of Kunming Medical University, Xichang, Kunming 650032,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_037024855.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 17:48:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,020
            CDSs (total)                      :: 5,897
            Genes (coding)                    :: 5,714
            CDSs (with protein)               :: 5,714
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 66 of 183
            Pseudo Genes (incomplete)         :: 124 of 183
            Pseudo Genes (internal stop)      :: 25 of 183
            Pseudo Genes (multiple problems)  :: 32 of 183
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP146197.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="kp1"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamedG"
                     /geo_loc_name="China:kunming"
                     /collection_date="2020-01-11"
     gene            66..242
                     /locus_tag="V8G91_RS30050"
                     /old_locus_tag="V8G91_30050"
     CDS             66..242
                     /locus_tag="V8G91_RS30050"
                     /old_locus_tag="V8G91_30050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            232..576
                     /locus_tag="V8G91_RS30055"
                     /old_locus_tag="V8G91_30055"
     CDS             232..576
                     /locus_tag="V8G91_RS30055"
                     /old_locus_tag="V8G91_30055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            785..>1219
                     /locus_tag="V8G91_RS30060"
                     /old_locus_tag="V8G91_30060"
                     /pseudo=""
     CDS             785..>1219
                     /locus_tag="V8G91_RS30060"
                     /old_locus_tag="V8G91_30060"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1254..1763
                     /locus_tag="V8G91_RS30065"
                     /old_locus_tag="V8G91_30065"
     CDS             1254..1763
                     /locus_tag="V8G91_RS30065"
                     /old_locus_tag="V8G91_30065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            1770..1982
                     /locus_tag="V8G91_RS30070"
                     /old_locus_tag="V8G91_30070"
     CDS             1770..1982
                     /locus_tag="V8G91_RS30070"
                     /old_locus_tag="V8G91_30070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2649..2873
                     /locus_tag="V8G91_RS30075"
                     /old_locus_tag="V8G91_30075"
     CDS             2649..2873
                     /locus_tag="V8G91_RS30075"
                     /old_locus_tag="V8G91_30075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            2857..3123
                     /locus_tag="V8G91_RS30080"
                     /old_locus_tag="V8G91_30080"
     CDS             2857..3123
                     /locus_tag="V8G91_RS30080"
                     /old_locus_tag="V8G91_30080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3193..3543)
                     /locus_tag="V8G91_RS30085"
                     /old_locus_tag="V8G91_30085"
     CDS             complement(3193..3543)
                     /locus_tag="V8G91_RS30085"
                     /old_locus_tag="V8G91_30085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(3609..4154)
                     /locus_tag="V8G91_RS30090"
                     /old_locus_tag="V8G91_30090"
     CDS             complement(3609..4154)
                     /locus_tag="V8G91_RS30090"
                     /old_locus_tag="V8G91_30090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(4180..4506)
                     /locus_tag="V8G91_RS30095"
                     /old_locus_tag="V8G91_30095"
     CDS             complement(4180..4506)
                     /locus_tag="V8G91_RS30095"
                     /old_locus_tag="V8G91_30095"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(4864..5022)
                     /locus_tag="V8G91_RS30100"
                     /old_locus_tag="V8G91_30100"
     CDS             complement(4864..5022)
                     /locus_tag="V8G91_RS30100"
                     /old_locus_tag="V8G91_30100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     Typing          5497..5576
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(4914..5311)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          567..2098
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgtccaacg ccggagcgac ccggaaaact tttccgtgcc gtccggcgtt tgcgaaggag
       61 ggataatgca gaagccgaag aagaagccgc tcgtcgtaaa aaaaagatcc tggcatccgg
      121 cagtgatggc aaatctgatg cgtcaggcac aggaggaaga ttaccgccgg gagcagatgg
      181 cagagcttga gcgttcagcc cggtcacatt ttgcggatat cggaggagag gatggcggtt
      241 aagcgtaccc gttttttagg tatccgggtg accgatgggg agtaccagca gctgctggag
      301 cgctgcaatg gaaggcaact ggcggtgtgg atgcgggaaa cctgtctcga tacgcgcccg
      361 gcgcggtcgt tgcggctgcc gtccatcgat ccggttttgc tgcgccagct tgccgggatg
      421 gggaacaacc tcaaccagat cgcccggaaa attaacggcg gccagtggtc aggcgctgac
      481 cgggttcagg tggtggccgc gctgatggcc attgatgccg aactcgagcg gctgcggcat
      541 gccgtgctgg aaaagggggc agacgatgat cgttaagttt catccgcgag ggcgaggcgg
      601 cggtgccggg ccggtggatt atctgctggg gaaagaccgc cagcgcgacg gggccatcgt
      661 cctgcaggga aagccggagg aggtccggga gcttatcgac gcctcgccct acgccaaaaa
      721 gtacacctcc ggcgtcctgt cttttgctga acaggattta ccgcccggcc agcgtgaaaa
      781 gctgatggcg agcttcgagc gggttctgat gcccggactc gataaagacc agtacagcgt
      841 gctgtgggtt gaacaccggg acaaggggcg gctggaactg aattttctgg taccgaacac
      901 ggaactgctg accggcaggc gtctccagcc gtactacgac cgggcagacc gtccgcgcat
      961 cgatgcgtgg cagaccgtgg tgaacgggcg actggggctg cacgacccga acgcaccgga
     1021 gaaccggcgc gcgctggtga cgccatcttc gttacccaaa acgaagcagg aagccgcaga
     1081 ggctattacg cggggcttac tggccctcgc ctcgtcaggg gagcttaaaa cgcgtcagga
     1141 cgtcactgag gcgctggaaa gcgcaggttt tgaggtcgtg cgcaccacaa agagcagcat
     1201 cagcattgcc gacccggacg gagggcgaaa catccgactt aagggagcca tctatgaaca
     1261 gtcttttaac gctggcgaag gacttagagc agaaatcgaa agcgcagcag cagagtaccg
     1321 gcgaaatgct gaaagccgca ttcagcgagc acgagaagtc tgtcagagcg gaactgagcg
     1381 aaagcgggaa gagaatcagc gccgccatcc gcgcccacga gcagggtatg agcgcggcca
     1441 tgcagtcgaa ccgcctgagc gtgatgcgta tggtcgggcg gacatggctg accatcacga
     1501 tggtgtcagg actgctgttc gccagcctga gcggggtgtt gtggtatcag gggagcttga
     1561 tagcgtcaaa tctggccgaa atcgaccggc agaacgcagc gctgtcgaag ctgaacgcga
     1621 agacctgggg cgtgacgtac ctggaagaca gcaacggacg ttttctggtg ctgccgaagg
     1681 ggacggccgt agacaggacg cagagctgga cggtcgggaa cgggcggtcg aagcagaacg
     1741 cgctgagact ggtgaaggag taagtgccga tgaccgagct ggaaaaacag ttgctgagcg
     1801 cattagagca gctacagcag gactactcga aaaggctgga cgagtgggag agcgccttcg
     1861 gggaatggcg gaaaatgtca gggcttatgc aacgggagaa cgcggcgctg agcgagcgcg
     1921 tgacgcgctt gagtcagcag gtggaacgct tgagcgggca actgcagcgt ttgagccggt
     1981 agtgcagcgt catgagatgg ccgtggccgc agaacgggcg cacgagcaac ggcagcacga
     2041 aaaggaactg acggaagcgc gttcccggca gcgaagctat gacggtccgt cgctgggctg
     2101 aaatgcagga aaacagggtt ctcatcgggc atttacgctg aaaccggatg tcattcacct
     2161 ggctgcatgg ctatgcagcc aggtaaaatt tatctgtcgc gaacggggcg gtttatcggg
     2221 tggtttgttg ccggttttac ctgactgcca ccccgttcgc ggcgaacccg tccggggcgg
     2281 tgcgggcaac gggtgttatg tttaatcagc gtgaacggca gatacaaaaa agccccggag
     2341 ggcttttaac tactgactta tttttttgaa gatttgctgg gtttcaattt gtcagtattt
     2401 cccggcgttt ttttattgtc acgctggcgt ttatcaggtt cccctgttga atcggcaatt
     2461 cgtttcggac ccggcttaag acccataatc gttatccttt caatagttaa gaggaaagcc
     2521 ctcaagctag atatattgac cgcgttacgg attatcaaga ggggctaaca agattatttt
     2581 ttgttctttt aggttgattg tggtactgtt gatatacatg tatagcaaat ggtgttcggg
     2641 aggtttatat gcttgctatc cggttatctg atgagattga gtcccgtctg gactcgctgg
     2701 cgaagcaaac cggcagaaca aagacgtttt atgcgcggga agcaatactg gcgcatctgg
     2761 aagacctgga ggattattat ctttcagcag aaactgctgc acgcgttcgc cgtggtgatg
     2821 aagcagtgca ttcgtctgaa gacgtgagga agtcacttgg tctggacgat taactattcc
     2881 gatcgggcgc tcaaatcgtt acgcaagatg gacaaacaga acgcacgacg gattgtggat
     2941 tttatgagtt tacgcattgc agttgctgcc gatcctcgcc agtcagggaa gccgctcaaa
     3001 ggtgagctgg gcgagttctg gcgctatcgg gtgggagatt atcgcgttct gtgtgagatc
     3061 cgagatgacg agcttgttat ccttgccgcc acgattggac atcgccgcga agtttacgac
     3121 tgaagcccgc tatgcgggct tttttgatgc cgacaacagg tattatggta aatactcgac
     3181 taactacgtg ttctactgtt tgtcggtttt agttaaccag caggttgtct taacgataat
     3241 gtagccgtta tttttaacta ataaggtcat attgttaacg tcaggcataa taaaagacat
     3301 tatggcctca gagttatcat cagaagcagc tctctgcatt actacttcga ttcccttttc
     3361 tttgaatcta taagtatcca gttgcctatt aaacttagaa agacctacaa ccatacttag
     3421 tttgttatct ttaacagtaa gagatgcttc ctgagtacca ttacatcggt agtaagcggt
     3481 gtaaccgtca gcaaaagatc gaccggggaa tataaatgct aggaacgcaa ttaaccaata
     3541 catagttgag aatcttggta ttacacattt cactatcctc tctttcattt ttcgttcacc
     3601 atactttgtt atctgatttt taatttttct attttataaa ccaagtctct accaaaaagg
     3661 cgaaaattat cctcaaataa taataattgt ttaagaacat ccatgaaagc agctgcttca
     3721 ccctcagtta tgggagctac tctatataac atataacctg agttaagggt ttcttttata
     3781 ctttcttcaa tgctcattat aaatattagt ggattatcat ttttcttccc ccatgggttt
     3841 ctaattaatc cttcgatgta ctcaggtcct ttgcgtccta ttgcctgcgc aatatttttt
     3901 atttcatctg cgtgagggct ttgttgtaca gctttgttta tgcctgcttg agtgattttc
     3961 agagactcta caccgtgtat gttgtatgag ataaaatgta ataaattaag atgcaatgca
     4021 attaatccat ttttgtttac atctgagtat ttggataaaa aacgtaatga agtgttaact
     4081 ctcccttgaa gcctaacaat tgggagtgct gccttgtata tttccttctc cgttaaatac
     4141 cgtttaaaaa acataattct ccgcctccgt atagcgtgcc tattttattt cgtcgtaaaa
     4201 cttagctctg aaatattcct ttagaaccat catgtcatta ataattgaat ctatctcatc
     4261 cattttttct tggtattctt ttaatggtga agggagcggt aactcaatat ttgaaaagtg
     4321 attggcgaga tagggatcaa ggtttctcat ccattcaggc ttttcgccaa tgcgttcttc
     4381 aactgaaagt gaacctgata atctctggtt aataagatca cttagactca ttttggtttt
     4441 attcgaaagc cgttgtaagg attgctttaa tcctatatct agcatgatat ttgttctttc
     4501 tctcattacc cccccttgct attgcgtttt ttgttgcgca caaagctacc attgcgcatt
     4561 aagaaacgca ataaaaaagg agggagggag tggtttgtac atgatgtgca gtgcgctgag
     4621 ggcatcagtg gcgggtgttt ttccgtggga tggatggagc tccggggata acagaactga
     4681 cgatcattta aaggatcttc ttgagatcct ttttttctgc gcgtaatctc ttgcactgta
     4741 aacgaaaaaa ccgcctaggg aggcggtttg atcgaaggtt aagtcagttg gggaactgct
     4801 taaccgggta actgggtata cgagaccgca gataccaaaa ctgttctttc agtgtagccg
     4861 cagttaggca cccacttcaa gacctctcaa tatctctctc gtatatatcc agttaccaat
     4921 ggctgctgcc agtggcgttt ttgcgtgtcc gtccgggttg gactcaagat gatagttacc
     4981 ggaaagggcg cagcagtcgg gctgaacggg gggttcgtgc atacagccca gcttggagcg
     5041 aacgacctaa accgagccga gataccagca gcgtgagcta tgagaaagcg ccacgcttcc
     5101 cgaaagggaa aaaggcggaa caggtatccg gtaaacggca gggtcggaac aggagagcgc
     5161 acgagggagc cgccggagag aaatcaccgg catctttaag tcctgtcggg tttcgtcctc
     5221 ctctgacttg agcgtccatt tctgtgatgc tcgtcagggg aggcggagcc tatggaaaaa
     5281 cggcaacgcc gcatcctttt ctccggccct ttctgatttg agcgcctttg ctttatcccg
     5341 ttatccgtga gtcggtcacg cccgccgcag ccgaacggca ggagcgcagc taccgagtga
     5401 gcgaggaggc gtcatttcat ctgtccgtgc atggtattgc ggacgctcct gagccacata
     5461 tcacacctgc cacatgaggc actttcatga atcgtgctct catgccaaca tagtaagcca
     5521 gtatacacag cgtaaacgct gtgactggtt cagggctgcg ccccgaaacc cgctaacacc
     5581 actgacgcgc tggcgc
//
