LOCUS       NZ_CP146030            10060 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain 50105 plasmid pKPN50105-6, complete
            sequence.
ACCESSION   NZ_CP146030
VERSION     NZ_CP146030.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39981283
            Assembly: GCF_036967225.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Yang,W., Ma,X., Xu,H. and Hu,F.
  TITLE     Klebsiella pneumoniae strain 50105, complete sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Yang,W., Ma,X., Xu,H. and Hu,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-FEB-2024) Institute of Antibiotics, Huashan Hospital
            of Fudan university, No. 12 Urumqi Middle Road, Shanghai 200040,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036967225.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 17:39:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,960
            CDSs (total)                      :: 5,836
            Genes (coding)                    :: 5,681
            CDSs (with protein)               :: 5,681
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 49 of 155
            Pseudo Genes (incomplete)         :: 108 of 155
            Pseudo Genes (internal stop)      :: 18 of 155
            Pseudo Genes (multiple problems)  :: 19 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP146030.1.
            Bacteria and source DNA available from Institute of Antibiotics,
            Huashan Hospital of Fudan University, No. 12 Urumqi Middle Road,
            Shanghai, Shanghai 200040, China.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="50105"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKPN50105-6"
                     /geo_loc_name="China: Xiamen"
                     /lat_lon="39.69 N 115.99 E"
                     /collection_date="2023-04-29"
                     /collected_by="Xiaobo Ma"
     gene            join(10035..10060,1..232)
                     /locus_tag="V6N03_RS29740"
     CDS             join(10035..10060,1..232)
                     /locus_tag="V6N03_RS29740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            317..466
                     /locus_tag="V6N03_RS29745"
     CDS             317..466
                     /locus_tag="V6N03_RS29745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            1950..3914
                     /locus_tag="V6N03_RS29750"
     CDS             1950..3914
                     /locus_tag="V6N03_RS29750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            3914..4645
                     /gene="mobC"
                     /locus_tag="V6N03_RS29755"
     CDS             3914..4645
                     /gene="mobC"
                     /locus_tag="V6N03_RS29755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            4652..5182
                     /locus_tag="V6N03_RS29760"
     CDS             4652..5182
                     /locus_tag="V6N03_RS29760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5209..5388)
                     /locus_tag="V6N03_RS29765"
     CDS             complement(5209..5388)
                     /locus_tag="V6N03_RS29765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5414..5842)
                     /locus_tag="V6N03_RS29770"
     CDS             complement(5414..5842)
                     /locus_tag="V6N03_RS29770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(6006..6320)
                     /locus_tag="V6N03_RS29775"
     CDS             complement(6006..6320)
                     /locus_tag="V6N03_RS29775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6308..6586)
                     /locus_tag="V6N03_RS29780"
     CDS             complement(6308..6586)
                     /locus_tag="V6N03_RS29780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(6761..7126)
                     /locus_tag="V6N03_RS29785"
     CDS             complement(6761..7126)
                     /locus_tag="V6N03_RS29785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(7123..7494)
                     /gene="tolA"
                     /locus_tag="V6N03_RS29790"
     CDS             complement(7123..7494)
                     /gene="tolA"
                     /locus_tag="V6N03_RS29790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(7768..8013)
                     /locus_tag="V6N03_RS29795"
     CDS             complement(7768..8013)
                     /locus_tag="V6N03_RS29795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8340..10025
                     /locus_tag="V6N03_RS29800"
     CDS             8340..10025
                     /locus_tag="V6N03_RS29800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             3340..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(570..1148)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3915..4642
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 gtttgataag aaaactgaag agtttaaagg ggaagagtat tctaaagact ttggtgacga
       61 tggttctgtc attgaaagct tgggaatgcc tttaaaggat aatattaata atggtgagtt
      121 tgatttgaag aaagaatggg ttcctttact acagccattt tttaaaaatc aaataaaacc
      181 tgatgtggat aattgttata tagcttttga ttatcgggac ggtaactggt aacaaaaaat
      241 tatcaaggat gatttgaaat actgacttcc ggtgttcagg gatgaatgcc ggagtcttta
      301 aatacaggga aagtatatga aaaaggtaaa gactattttt ctttttatat tgattgttgc
      361 tggctttctt ctggccgcat gtcaggcaaa ttatatccgg gatgttcagg gcggaacggt
      421 ggcaccatcg tcctcctctg aactgacggg gatcgcggtt cagtagaaaa gatcaaagga
      481 tcttcttgag atcctttttt tctgcgcgta atcttttgcc ctgtaaacga aaaaaccacc
      541 tggggaggtg gtttgatcga aggttaagtc agttggggaa ctgcttaacc gtggtaactg
      601 gctttcgcag agcacagcaa ccaaatctgt ccttccagtg tagccggact ttggcgcaca
      661 cttcaagagc aaccgcgtgt ttagctaaac aaatcctctg cgaactccca gttaccaatg
      721 gctgctgcca gtggcgtttt gcgtgctttc ccgggttgga ctcaagtgaa cagttaccgg
      781 ataaggcgca gcagtcgggc tgaacgggga gttcttgctt acagcccagc ttggagcgaa
      841 cgacctacac cgagccgaga taccagtgtg tgagctatga gaaagcgcca cacttcccgc
      901 aagggagaac ggcggacagg tatccggtaa acggcagggt cggaacagga gagcgcaaga
      961 gggagcgacc taccggaaac ggtggggatc tttaagtcct gtcgggtttc gcccgtactg
     1021 tcagattcat ggttgagcct cacggctccc acagatgcac cgaaaaagcg tctgtttatg
     1081 tgaactctgg caggagggcg gagcctatgg aaaaacgcca ccggcgcggc cctgccgttt
     1141 tgcctcacat gttagtcccc tgcttatcca cggaatctgt gggtaacttt gtatgtgtcc
     1201 gcagcgcccg ccgcagtctc acgcccggag cgtagcgacc gagtgagcga ggaggcgcaa
     1261 tttccccggc acctgcaaga cggtcaccgg acgcgctgat gtgggatatt gtcccctgat
     1321 taagtctttc tattccctga cgatcaatta tcaggatagg tgaaccatgt cggtttttcg
     1381 ctgaacattt tacgttaacc tatctctgtt gcttttttct ccttgcggag aaatgcaagt
     1441 catccccgct ggggcgtggc tgacttcagg ggaaaagacc ttgcttacgc tggctggacg
     1501 ccaccgacgc acggtctttt cccctctttc tggctctgtc cggctggacg ccggccacgc
     1561 cagaaattca cccggccccc gtcaggctca cgcctgacca cccacaaaaa tccacggtgc
     1621 tggacgcacc tgacgaagca gcaaagctgc tcctgatttt gcgggtggtc agccgctcgc
     1681 tatggcgaac tctgacgggg gccggaacac cggaaaagac cgcactgggg tgccgccttt
     1741 tcctctcaca cgctggggcg tgctcgttgt tcctccgggg ctgcgccttt tccgcatggg
     1801 gcggaaagag gctggcctcc gcggtgaaag ctgaacgcaa tcaccacgga cgtgaccgcc
     1861 gccgccatgc cttggggcat gtccgcggct tgtgcagcgg gggctgcgca tcacgccggt
     1921 aaacccgtcg tctgaaggag agagattgca tgtttaatac ggattcgctt gcctggcagt
     1981 ggcatatccc ggagctggcc cggcagattt acccgtgggt cagctggctg taccgggagt
     2041 attacagtct ggccggtttt tttctggtta tcggcctgtt tttcggtctg gctgaactct
     2101 gggccggccg aatgcgtttt gtggggctga gccataaggc gcggcgggag tatgccgatc
     2161 gctataagcg cgataataac cggactgtgc cattttcggt gctggcctcg ctggattcac
     2221 ccgggcggat ggtcagggca cggcgacatg tctggaacta cgcgtactgg ctgaccggcg
     2281 ctgccggtct ggcccttctg ggggggctgg ttgtcgccgt gtttatcgag agctggggag
     2341 aaaccgtcct gcgcccggac ctctatgcga acgcagatta ttacctgctg cagccggcta
     2401 acctgttcgg gatagcctgg tgtgccggag ggcttgtcgc cggcgtcctt ctggcgcggc
     2461 tgctggcgtt cctgctgctt gatggtcatt ttgcggccgc tgacgaggcg gtgaacgcga
     2521 aactgaatca ggagagccgg cgcagcagcc agcggaccgg agaaatgaca gacgtccgcc
     2581 acctgcattt cggggagccg gtaccggtta acgcgctggc cgatttttcc acggagcagg
     2641 cccggaaaca gcaggcggtg tttctgggta aggatgagca gggccagccg gtgctggtcc
     2701 ctcgtgatac ctggcgcaag accaatattc agatcctcgg cctgcccggg agcggcaaga
     2761 gcgcgatggc caccaacgcg ctcattcgct gcgtacgcga tttcggggac gctgtggtgt
     2821 acttcgaccc caaaggggac gcgtgggcgc cgcacgtctt ccgggcgcac tgcccggact
     2881 tcaccctgct ggatctgcgg ccggggaaac cggcgcagct gaatctgttc cgcgatctcg
     2941 accagtacgc cctgaagaat ctgctggttg ccgggtttaa cctgagtgag accggcgacg
     3001 tggcggacca ctaccgcatc agcgagcaga aagcggcgaa gctgattgcg gagcagttcc
     3061 cgcagggggc aaacattcag caggtgctgg cggcggcgta cgcgttgccg gaggccctga
     3121 aaaaggacgt gaaggggctg atcaccaagc tggagaacgt ggccgacctg agcgtcctgc
     3181 agacggacag cggtatcgat gtggcgggga taatcaacgg cggcggctgc ctgtacgtca
     3241 tcggctcgat ggacgatgag gcggtgatcc gggtgcagaa gatgctgttt gcccgctgcg
     3301 cgcaaatcat cattgcgcgg gatgagttcc ggaggtggcc gcacgcgagc atcatgctcg
     3361 atgagattaa atatctgctg tcgaagtacg tgctgaacgc gctggggacg ctgcgcagcc
     3421 gggactgcaa cctgctgctc gcgcaccagt cgctgggcga cttcggtcag tgcgggcagg
     3481 atctgcccgc tgattttgtg aaaaccacgg tactggataa cacgccgatc cgctggttct
     3541 atcgcgcggc cagccaggag tccgcgcagt gggcggcggg ccagaccggg gagatccggg
     3601 tggacgtgga gcgccgccgg gccagccggg aggcggggaa cgtggagcac atcagcggcg
     3661 acagcttcat ccagaaggag gcgcggccgc tgtttgacgt gaacaccctg caacacctgc
     3721 ctgacgggtt cgcggtgatg accgggctgg gcgtggcgcg gctggcgttc agcagcccgc
     3781 tgcgggttga gcgccgggaa atttcgctga aaaccttccc ggtgctggcg aaaacggacc
     3841 cgctggcgga gtacctgcct ccggccgacg gcggcggacc aaaagcggat gatgattttg
     3901 cggggcttta ctgatgacac tggagcagta caacgtcagc cacgcgaagc ggcaggcgcg
     3961 gaacgcggag aaaacacggc tgaccctgcg ctggctgcgg gaagagctgt gttccacggc
     4021 ggagctggtt gcgcgacggc tggggattgc ggcggtacag ccggtttacc gcttcctgga
     4081 cagtctggtg gcgaaagggc tgctggtccg ggcgaaatac ccggttgacg gccgtcaggt
     4141 cagcgtgtgg gggctgacgc cgcacggggt ggcgttctcg ttcgacgagg acgagccgct
     4201 gacggacgtt attccgtttc agccgtcaag ggtgtcggcg gcgcagctgc cgcaccggct
     4261 ggccgtgcag tccctgcgcc tctccatgga gggccggggg gccaccggct ggcggtacct
     4321 gcaccgtatg gccctgaaag ggatgaaagt gccggacgcg ctggcggagc tggacggcag
     4381 aacggtggca tttgaggtag agcgcaccgt caagagccgc aggcggtatc aggaagtggt
     4441 gtccgggtat ctgttcaacc gccgggcgaa cggcatcgat gaaatctggt acatctgccc
     4501 tgaccggccg acgcttgtcc gggtacagcg ggcgattctg gcggtggatg aaatcgtgaa
     4561 tccacagacc ggcgaggccc gcaaaacggt ggagctggac cgggagaggc tgttcgcgtg
     4621 ctttaaattt atgacaacgg agtgagggat tatggcgctg tacatggtat ttattgagga
     4681 cgtggaagac aaagacggcg agcgctgcat tatgccaacc agcgagcatc tggaagacat
     4741 ggtggtgctc gcctggcaga ccggggcgta tgttttctgt tttgtcgacc gggcccagca
     4801 tctggcgatg atgaaatcca tcgggatgta tgacgagcgg ctcaacagcg acgaaaaagc
     4861 cacgtacttt ttcaccggtg aaaatccgca atgggaacgg gacaaaccca caggaatgaa
     4921 gctgtgtgat tacctctatc gaaaggtgga gcggttcgcc atcgagaacg gtgtgcaggt
     4981 ggttgatgcc gggaagtgct caccggagca gattaacgcg atgattgagg tggtcagcga
     5041 gcggcacggg gaaagcgcgg tgaacgagaa gctgaaagcg gagctgatgc gggaatataa
     5101 cagccgctat tttaacgtgc cggtgcctga actgccggag gacaaacccg cccgccggtg
     5161 gtggccgttc ggcaaaaaat aacgccagca ccggcatggc cggtgctgtt attcttcccc
     5221 gagcttatcc cgcatcacct gccagagtgt ttccgcatcc tcgtgaagcc gttcgcacat
     5281 gtcggccggg gtatccatgt ccagctcgtt cagcttttcc agcaacagca gcgactgatc
     5341 cttaatgaat ttagccatat tgagggcagt tttttcctgc ttcgtcatgt tctccattcc
     5401 cttcggtaag ttctcagcta aaccagccgg accagaagcg ttttttcttc tgtgtcagtt
     5461 ctgttgtcaa tctgtccact tcggcctgga gtttccggcg ctcatcatcg tgctggcggc
     5521 gttcttcttc ccgggatgtt ttatcttcca gcatcagggt gacagcctgc cggagatcgg
     5581 ccagttcctt ttgcattgcc agaagctgtt cctgtgggaa atttttttgt gtattttcac
     5641 ggtgtgaaat gccgggaatt tgtttctcag ggatattcac atttccgtat acacggatca
     5701 gttctgacac atccacgaca gggttgtttt tcccgtcacg tgacacggta acctttccct
     5761 gttttacatg attgtatagc gttcttctgg taatgcccgc ggcttgtgct gcctgtgaaa
     5821 ggttgagcaa tgttttcgcc actgtatacc cctgatttta tcgggtgtga aaggtgtgta
     5881 atttctcacg gtaaatgtat cgctgaattt tacccgtttt tccgttcagg atgtggtttt
     5941 ctgacgcgct gcgcttgtca aaccggcgac ggccacgcaa tgaattgcgc ccccgtcatc
     6001 ggttattaga ggctgtctgc ccccggatac ccggattctt tctgatgcct gacggtgacg
     6061 atcacaatct ggtccatttc ccggtcgtgg cggtagagca tcacatagcc actgtcgcca
     6121 aacccgatca ccagttcctt atattccagc ggcagaaacg gcaccgggcg gccaatgtct
     6181 ggcaatgttt tcagttgctg gatagcccgg acgatcacct cacctccttt tctggctgcc
     6241 agccgatttt tggttttgag aaagtcctga aggcgttgta aatcgcgctg tgccagcgcg
     6301 gaaatcgtta cctgtggcat ggcggagcat cctgctcgtt ctcagttccc caggtactta
     6361 tccaggcttc cgcttcttcc gcagtaaggt gcagacccgt ttcctgatag tgctgccatg
     6421 cggcctgtcc gtcccgcagg tactggctgc gcttctcttc ccggtcgatg tattcggtga
     6481 ttgcctccag cattagcgcg tgcgctgagc ggtgccggtc atcggccagc gttttcagcc
     6541 ggtccttaag ttcttcatcg agcctgatgg atgttgctga tgacataaaa gcccctttgt
     6601 agtcatatgt attacacatg actactttaa tccgtggcgg cgctatttgc cagaaatacg
     6661 gcttttgctg cccatttttc agcttcgctt ccactaatca gggctgctac aggctcactc
     6721 atcatccagg actattgcag cctgcgggtt aaaggacctg ttactggcgg caaccatcat
     6781 cattgagctt ttgggtggtg actggtcgcg gatcctgtaa gccgtcattt ttcagactga
     6841 attcaaaatt tataacctgt cgcattgccg ggtgccctgc gggatatagc cagtgcctca
     6901 ttgccgtcag cgtttcccga aaaaaaaggt gacagccggt aagcgacacg ttgtccactc
     6961 gtccatcgtc cagaatatca aacgtggcgg tcacctgacc ttcgatgcgt tttgctgcgg
     7021 cctgtaccgg gtaggttggc tgcagtgtgg ttacgggttt tagtggtatc gcatgtaccg
     7081 gagtgaggaa catggccgcc agcactgtca gagtaaaagc tttcatggtc tgaaatccag
     7141 aggggcgttt ttgaatattt gccaggtatt ttcatccggt gccggcggaa ttttggcgtg
     7201 ggtaacggct gatattgctg ctttacagag tttcggatct cctccttcag caatggcgct
     7261 gttcagagtg ccatcacgtt gtatggatat ccgcacggtg cacatttttc ccgcataggc
     7321 ctctggttca aacatctgca tttcaacagc ggtcttgatg gccgcagcgt agctactgat
     7381 tttagatgac ttatccagag tataaatttt cggcggctgg gtggtggaat gcttacaggc
     7441 cgtcaggagt aaggggctgc acaatgccag caggataaag ggtattaaac gcatcgggtt
     7501 gatgacctgt cagtaaaaaa tgttacatgt atatttgtca ctctttacgg ccacgttcat
     7561 ttacataaac acggcttatc gtatcgtttt tacaccaaac ggcccgccat gcagttgcgt
     7621 gcataggcat gcagcgggtt acaatttacc gggcgcgttt ccggcggttt tccgggtggt
     7681 ttgttgcctg ttttatcccg tagccgccgg aaacgccctc agaccgtctg agcggtgcgg
     7741 gcaatgcgac gttatggtaa atacccctta ccggcggcgc tgtggctgcc aggtgaaggt
     7801 ggtttccgta gcctcgtatt tctcccgacg catttcctgc gcccgcctgg cttcacgttc
     7861 gtcctgaatg gcacgaagca ccctttcccg cacctgttgt tttaccgcct ttggcagcgt
     7921 tttgttccct ttcaggccca tggccagtgc ctgagcgaca aaggcgtccg ccaggcgctt
     7981 ttcttcagcg ggagtcagtt tcaggtgcgt catagtggcc tccgggcgtc tcaggtttcc
     8041 cgaacagttt aaccgcaggt cctctcctca tgccagttag ctttcagatg tgtctatacc
     8101 atctgaaatg taagtgtgat gaatgtctta aataataaaa tttgttgcca tgagaaattt
     8161 tcgggaaaag acgcgatctg cttacaggtg gctccgggcg cttttttgtg cctgaagatg
     8221 agaactaatc tcatatattg acacctgaaa acgggaggag taaggtaata attatactgt
     8281 gtatatgtac agtatgttgg ctttaattta ttgttttaaa tgtcaaagag gaaaacggta
     8341 tgagtggtgg agacggtcga ggtccgggta attcaggtct gggacataat ggtggtcagg
     8401 ccagtgggaa tgtgaacggt acgtctggta aaggtggccc ttcatcaggt ggtggtacgg
     8461 atccaaacag cgggccgggc tggggtacga cgcatacgcc gaatggagat attcataact
     8521 ataatccggg ggagtttggt aacggcggga gtaaacccgg tggtaatggt ggtaacagcg
     8581 gcaatcatag cggtagctct ggtggcggac agtcttcggc cactgcgatg gccttcggtc
     8641 tgcctgccct ggctactccg ggggctgaag gactggcttt atcagtttcc ggcgatgcgt
     8701 tgtcggccgc cgttgctgat gtgctggctg ccctgaaggg gccgtttaag tttggtctgt
     8761 gggggattgc gatctacggt gtgctgcctt ctgagattgc aaaagatgat ccgaaaatga
     8821 tgtcaaaaat tatgacgtca ttaccggccg atgcggtgac ggagactccg gcaagtactt
     8881 taccactgga ccaggcgacg gttcgtgtca gacaacgggt tgtggatgtg gtgaaggatg
     8941 agcggcagca tattgcggtt gtcgcaggtc ggccaatgag tgtccctgtg gtggatgcga
     9001 aaccgacaaa acgtccgggg gtattcagtg tgtcgattcc gggtctcccg tctctgcagg
     9061 tgagcgtacc gaaaggtgtt ccgacagcga aagccccgcc aaaaggcatt gttgctgaaa
     9121 aaggtgattc acgtccggct ggttttacag ccggtggtaa ctcccgtgag gccgttattc
     9181 gtttcccgaa agagaccgga cagaagccgg tttatgtgtc ggtgacagat gttcttaccc
     9241 cggcacaggt aaaacagcgt caggaggaag aaaagcgtcg ccagcaggca tgggacgccg
     9301 ctcatccgga agaggggctg aaaagagact atgataaagc gaaagctgag ctggatgccg
     9361 aagataaaaa tattgcgacc ttaaacagcc gcattgcatc gacagagaag gcgctccccg
     9421 gtgcaagggc tgctgttcag gaagccgata aaaaggtaaa agaggcagag gcaaataaag
     9481 atgattttgt gacttataac ccgcctcatg aatatggctc cgggtggcag gatcaggttc
     9541 gctatcttga taaggatatt cagaatcaga atgagaaatt aaaagcggct caggcatctt
     9601 taaacgcaat gaatgaatcc ttatccagag ataaggctgc acttaccggg gcgatggaga
     9661 gccggaaaca aaaggagaaa aaagcgaagg atgcagaaaa taagttaaat gaggaaaaga
     9721 acaagcctcg caaggggact aaagactacg gccatgatta tcatccagta cctaaaacag
     9781 aagacataaa gggacttgga gagttaaagc gaggtgatcc taaaacacct aaacaaggtg
     9841 gtggtggtaa gcgggcccga tggtatggcg ataaaaaacg taaaatttat gaatgggatt
     9901 cccagcacgg tgagcttgaa gggtatcgtg ccagtgatgg cgaacaccta ggcgcatttg
     9961 atcccaaaac gggtaagcag gttaaagggc cagatccaaa acggaacatt aaaaaatatc
    10021 tttgagaggt aaatatgggg cttaaattac gtttgacatg
//
